Figure 7.
Inhibition of NK function by transient transfection with dominant-negative MAPK/ERK2 in YT effector cells. Equal aliquots of YT cells were left untransfected or transiently transfected with KD MAPK/ERK2 in which K52R substitution was constructed, or with mutant ERK2 where Thr and Tyr residues in the TEY motif were replaced with glutamic acid (TEYE) or with alanine and phenylalanine (TAYF). The wild-type ERK2 plasmid (WT) was also used to transfect YT cells. After 24 h at 37°C, untransfected and transfected YT cells were assessed for viability and adjusted to the appropriate concentrations of viable cells before testing for lysis of 51Cr-labeled Raji tumor cells at the indicated E/T ratios. The SEM of each mean percent cytotoxicity was <5%, and was not shown.