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. 1987 Feb;169(2):742–750. doi: 10.1128/jb.169.2.742-750.1987

DNA sequence and in vitro expression of the B875 light-harvesting polypeptides of Rhodobacter sphaeroides.

P J Kiley, T J Donohue, W A Havelka, S Kaplan
PMCID: PMC211842  PMID: 3027044

Abstract

The genes for the Rhodobacter sphaeroides light-harvesting B875-beta, and B875-alpha polypeptides (pufB and pufA) are closely linked to the genes for the reaction center L and reaction center M polypeptides (pufL and pufM) on what has been termed the puf operon (gene order, pufB, A, L, M). The DNA sequence of the pufB and pufA structural genes from wild-type R. sphaeroides 2.4.1 was determined and aligned with the available amino acid sequence of the wild-type B875-beta and B875-alpha polypeptides. The relative levels of the B875-beta and B875-alpha and the reaction center L and reaction center M polypeptides synthesized in a homologous cell-free transcription-translation system were compared with those found in vivo. Analysis of the gene products produced in vitro with plasmids containing deletions upstream of the pufB structural gene identified a region of DNA required for expression of the B875-beta and B875-alpha polypeptides. These results support the hypothesis that the mapped 5' termini of the large and small puf operon transcripts represent transcription initiation sites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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