FIG. 1.
Association of the BCCV N protein with actin filaments. Vero cells were infected with BCCV (at an MOI of 0.5) and examined by IFA with anti-BCCV rabbit serum either at 3 days (A and B) or at 6 days (C) postinfection. (A) Filamentous pattern of viral antigen in infected cells without treatment. (B) Absence of the filamentous pattern in infected Vero cells which were treated with cytochalasin D. (C) Vero cells infected with BCCV (at an MOI of 0.5) at 6 days postinfection. (D) Uninfected Vero cells. Colocalization of BCCV N protein with actin microfilaments was examined in Vero cells that were seeded on coverslips and infected with BCCV (at an MOI of 1). At 3 days postinfection, the cells were analyzed by double immunofluorescence with anti-N MAb and phalloidin. (E) Anti-N staining, some of which (arrows) coincides with anti-actin staining observed with phalloidin. (F) Anti-actin staining with phalloidin.