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a) Wildtype barnase electrostatic surface at isopotential of +1 kT/e. b) Barnase nullified at residue 59, electrostatic surface at isopotential of +1 kT/e. c) Overlap of wildtype (transparent yellow) and nullified barnase (green) surfaces. a,b,c) The red square encompasses the main difference in isopotential surface due to nullification. d) Wildtype barnase (blue) in complex with barstar (transparent yellow).