Figure 3. AZD8055 selectively triggers apoptosis in XL413-induced senescent cells.
a, Hep3B and Huh7 cells were treated with 10 μM XL413 or vehicle for 10 days prior to sequential exposure to increasing concentrations of AZD8055 for 5-7 days in colony formation assays. b, Apoptotic cells were determined by caspase-3/7 apoptosis assay 96 h after AZD8055 treatment. c, Control cells and XL413-induced senescent cells were treated with AZD8055 for 48 h prior to western blot analyses with the indicated antibodies. d, Control cells and XL413-induced senescent cells were treated with AZD8055, and cell lysates were collected at the indicated time points prior to western blot analyses with the indicated antibodies. e, f, Long-term colony formation and caspase-3/7 apoptosis assays showing the synergistic effect of mTOR and SHP2 inhibitors on Hep3B proliferation. g, Hep3B cells were treated with AZD8055, SHP2 inhibitor or the combination of both drugs at the indicated time points prior to western blot analysis with the indicated antibodies. For gel source images, see Supplementary Figure 1. Data in a-f are representative of three independent biological experiments. Data in g are representative of two independent biological experiments.