Systemic Delivery of Anti-miRNA for Suppression of Triple Negative Breast Cancer Utilizing RNA Nanotechnology

Construction and Characterization of Triple-Functional pRNA-3WJ Nanoparticles

The pRNA-3WJ nanoparticles utilize a modular design composed of three short fragments (Figure 1A).³⁸ Upon mixing the individual strands in equal molar ratio in PBS or TMS buffer, the complex assembles with high efficiency, as shown in our previous publications.^38,39,48,59 Each branch of the pRNA-3WJ can harbor a functional module without interfering with the folding of the core scaffold and the function of each module, as demonstrated by atomic force microscopy (AFM) images showing homogeneous triangular branched architectures (Figure 1B,C). Herein, we used the pRNA-3WJ core as a scaffold and constructed trifunctional RNA nanoparticles 3WJ-EGFRapt/anti-miR-21, harboring EGFR targeting RNA aptamer, therapeutic anti-miR-21 and Alexa-647 as imaging module (Figure 2A). When the four strands were mixed in stoichiometric ratio, the RNA nanoparticle assembled with very high efficiency as indicated by gel shift assays showing stepwise assembly of the complex (Figure 2B).

Dynamic light scattering (DLS) assays showed that the average hydrodynamic diameter of 3WJ-EGFRapt/anti-miR-21 nanoparticles was 14.8 ± 2.6 nm (Figure 2C) compared to 4.2 ± 1.1 nm for pRNA-3WJ core scaffold (Figure 1D). We note that the 3WJ-EGFRapt/anti-miR-21 nanoparticle is not globular in shape, and deviations from DLS measurements are expected, since the reported DLS size corresponds to the average of the three dimensions due to rapid tumbling of RNA nanoparticles in solution.

The particle surface charge, zeta potential, was determined to evaluate the aggregation propensity of RNA nanoparticles in solution. RNA nanoparticles are indeed highly negatively charged and do not aggregate in solution, and this is reflected in the zeta potential measurements showing a single peak at −16.1 ± 7.7 mV for pRNA-3WJ core scaffold (Figure 1E) and −17.0 ± 5.6 mV for 3WJ-EGFRapt/anti-miR-21 nanoparticles (Figure 2D). This aggregation-free physical property and anionic nature is particularly attractive for in vivo delivery applications, since it minimizes nonspecific cell entry, and entrapment by lung macrophages and liver Kupffer cells.¹¹

To make the 3WJ-EGFRapt/anti-miR-21 nanoparticle chemically stable in vivo, we used 2′-F modified U and C nucleotides.^60,61 The 2′-F modified RNA nanoparticles were incubated with 50% fetal bovine serum (FBS) at 37 °C. At defined time points, samples were collected and assayed using native PAGE gel (Figure 2E). The fraction of intact RNA nanoparticles was quantified using ImageJ software and plotted to determine a half-life of about 18.5 h, which is significantly higher than the half-life of unmodified RNA counterparts.^38,60 The presence of 2′-F nucleotides not only makes the RNA nanoparticles resistant to RNase degradation, but also enhances the melting temperature of pRNA-3WJ,⁴⁰ without compromising the authentic folding and functionalities of the core and incorporated modules.^37,38

The 2′-F modified 3WJ-EGFRapt/anti-miR-21 nanoparticles were subjected to temperature gradient gel electrophoresis (TGGE) assay, typically used to determine one of the thermodynamic parameters, the apparent melting temperature (T_m), of RNA nanoparticles composed of multiple strands.^41,48,62,63 One of the strands was labeled with Alexa-647, which was used to determine the fraction of intact particles remaining with increasing temperature gradient (from 25 °C → 80 °C) applied perpendicular to the electric current (Figure 2F, boxed). The fraction of RNA assembled was quantified using ImageJ software and the melting curve was fitted with nonlinear sigmoidal fitting to determine an apparent T_m of 54 ± 3 °C (Figure 2F). The results indicate that the constructed RNA nanoparticle with all the functional modules are thermostable and will remain structurally intact at ultralow concentrations in the body.

Binding and Internalization of pRNA-3WJ Nanoparticles into TNBC Cells

Alexa647 labeled 3WJ-EGFRapt/anti-miR-21 nanoparticles were incubated with MDA-MB-231 cells. The cells were then fixed with paraformaldehyde, and the nuclei and cytoplasm were stained using DAPI and Alexa488-phalloidin, respectively. Confocal microscopy images showed very efficient binding and internalization of pRNA-3WJ nanoparticles into cancer cells through EGFR mediated endocytosis, as demonstrated by excellent overlap of fluorescent RNA nanoparticles (red color in Figure 3A) and cytoplasm (green color in Figure 3A). Very low signal was observed for control groups (3WJ scaffold only and 3WJ-anti-miR-21 without EGFR RNA aptamer). The results were further validated using Fluorescence-Activated Cell Sorting (FACS) assay (Figure 3B). The 3WJ-EGFRapt/anti-miR-21 nanoparticles (and controls) were incubated with MDA-MB-231 cells, washed and then analyzed by FACS. Strong binding (76.7%) was observed for EGFR RNA aptamer bearing RNA nanoparticles compared to pRNA-3WJ scaffold control (16.3% binding) (Figure 3B). The results indicate that these RNA nanoparticles have high specificity and affinity for TNBC cell binding.

Delivery of Anti-miR-21 to TNBC Cells by pRNA-3WJ Nanoparticles

We next tested the specific knockdown of oncogenic miR-21 in MDA-MB-231 cells, known to express high levels of miR-21.⁵⁷ As anti-miR-21 agent, we used 8-mer (5′-GATAAGCT-3′) locked nucleic acid (LNA, conformationally restricted nucleotide analogues) that is complementary to the miR-21 seed region.⁶⁴ LNAs have been reported to bind to their complementary miRNAs with very high affinity and specificity, and are also resistant to exo/endonucleases.^61,65 Upon binding to the miRNA seed region, LNAs will trigger miRNA inhibition in a dose dependent manner.⁶⁴

For assaying miR-21 inhibition, we developed a very sensitive luciferase-based miR-21 reporter system. MDA-MB-231 cells were transfected with a reporter plasmid which contains a miR-21 targeting sequences at the 3′-untranslated regions (UTR) of Renilla Luciferase gene and a coexpressed Firefly Luciferase gene as the internal control. In native cells, the Renilla expression would be repressed, as the miR-21 bind to its 3′-UTR region and prevent the translation of Renilla Luciferase. As anti-miRNA LNA are delivered into the cancer cells, the LNA sequence will competitively bind to miR-21 that used to bind to 3′-UTR region of Renilla Luciferase gene and block its translation, thus resulting in an increased expression of Renilla Luciferase. The results demonstrated that 3WJ-EGFRapt/anti-miR-21 effectively delivered anti-miR-21 LNA sequence into MDA-MB-231 cells after incubation, as indicated by increased Renilla Luciferase expression in a dose dependent manner compared to the control 3WJ-anti-miR-21 nanoparticle without EGFR aptamer (Figure 3C). The successful delivery via incubation with cancer cells is a significant advancement in RNAi-based cancer therapeutics, since RNA therapeutics are typically delivered by transfection or microporation methods.

The functionality of miR-21 was validated on its downstream target tumor suppressors, PTEN and PDCD4 genes.⁵⁵⁻⁵⁷ After incubation with MDA-MB-231 cells, 3WJ-EGFRapt/anti-miR-21 nanoparticles up-regulated the expression of both PTEN and PDCD4, assayed by qRT-PCR at the mRNA levels compared to control 3WJ-anti-miR-21 without EGFR aptamer (Figure 3D).

Effects of pRNA-3WJ Nanoparticles on Growth and Apoptosis of TNBC Cells

Caspase-3 (cysteinyl aspartate-specific protease-3) is an early cellular apoptotic marker, and its activation can be used to assess whether cells are undergoing apoptosis. We found that 3WJ-EGFRapt/anti-miR-21 treated MDA-MB-231 cell lysates showed the highest fluorescence emission of caspase-3 fluorogenic substrate (Ac-DEVD-AMC) comparable to the positive control camptothecin (CPT), and in contrast to the control RNAs (anti-miR21, 3WJ-anti-miR21, and 3WJ-EGFRapt/Scramble). The results indicate that 3WJ-EGFRapt/anti-miR-21 nanoparticles can activate caspase-3 pathway and trigger cancer cell apoptosis (Figure 3E).

Specific Targeting of TNBC Tumors in Animal Models Assessed by Fluorescence Imaging of RNA Nanoparticles

To evaluate tumor targeting, 3WJ-EGFRapt/anti-miR-21 nanoparticles were systemically administered via the tail vein into orthotopic TNBC tumor bearing mice. Ex vivo images of normal tissues, organs, and tumors taken after 8 h showed that RNA nanoparticles specifically targeted and accumulated in the tumors, and little or no accumulation was observed in healthy organs and tissues (Figure 4C). In terms of tumor accumulation kinetics, RNA nanoparticles reached their highest accumulation 8 h postinjection and remained in the tumor thereafter for an extended period of time to trigger miRNA knockdown. Such distinct tumor retention behavior is due to the nanoscale size and shape of RNA nanoparticles that are favorable for enhanced permeability and retention (EPR) effects. Histological profiles of breast tumor sections revealed that “active” targeting 3WJ-EGFRapt/anti-miR21 nanoparticles (mediated by EGFR targeting RNA aptamers) strongly bound and internalized into cancer cells, as shown by strong association of RNA nanoparticles (red) with counterstained TNBC cells (Figure 4D).

RNA Nanoparticles for In Vivo Targeted Delivery of Anti-miRNA to TNBC Cells

We generated orthotopic TNBC tumors in nude mice using MDA-MB-231 cells expressing luciferase. Upon systemic injection of luciferin, we could visualize the cancer cells using bioluminescence imaging to measure the tumor size and quantitatively assess whether the systemically delivered 3WJ-EGFRapt/anti-miR-21 nanoparticles can down-regulate miR-21 and in the process inhibit tumor growth. TNBC tumor-bearing mice were injected with 3WJ-EGFRapt/anti-miR-21 nanoparticles for 5 times every other day, and the luminescence signal was measured to assess luciferase activity. The end point luminescence signal after 5 doses from the mice treated with 3WJ-EGFRapt/anti-miR-21 RNA nanoparticle were significantly lower than the control treated mice (Figure 4A). This was also evident in the tumor growth curve showing sustained inhibition of tumor growth by 3WJ-EGFRapt/anti-miR-21 nanoparticles compared to the vehicle control (Figure 4B).

To validate anti-miR-21 knockdown at the molecular level, tumor tissues were extracted and lysed. The miR-21 as well as its downstream target mRNAs of PTEN and PDCD4 were quantified by qRT-PCR assay at the mRNA level, and the expression of PTEN and PDCD4 was also examined by Western blot assay at the protein level. The data showed that 3WJ-EGFRapt/anti-miR-21 nanoparticles treated tumors have reduced miR-21 levels compared to control group (Figure 4E, left panel). The knockdown of miR-21 correlated with increased expression of both PTEN and PDCD4 at both mRNA (Figure 4E, right panel) and protein levels (Figure 4F) for the treatment group compared to the control group. Furthermore, 3WJ-EGFRapt/anti-miR-21 RNA nanoparticle treatment reduced cell proliferation in the tumor tissue, as revealed by decreased Ki67 staining (Figure 4G, left panel), and induced cancer cell apoptosis, as indicated by increased active caspase-3 levels (Figure 4G, right panel), compared to the control group.

Design and Construction of 2′-F Modified pRNA-3WJ Nanoparticles

Multifunctional pRNA-3WJ nanoparticles were constructed using a bottom-up self-assembly approach.³⁸ The 3WJ-EGFRapt/anti-miR-21 consisted of four fragments (Figure 2A) harboring EGFR targeting RNA aptamer (EGFRapt) as a targeting ligand; AlexaFluor 647 (Invitrogen), as a fluorescent imaging module; and anti-miRNA-21 LNA (anti-miR-21) (Exiqon), as a therapeutic module. The controls include RNA nanoparticles without targeting ligand (denoted as 3WJ-anti-miR-21), without therapeutic module (denoted as 3WJ-EGFRapt), or without therapeutic and targeting modules (denoted as 3WJ).

The core sequences of pRNA-3WJ are as follows³⁸ (Figure 1A):

a_3WJ: 5′-UUG CCA UGU GUA UGU GGG-3′;

b_3WJ: 5′-CCC ACA UAC UUU GUU GAU CC-3′;

c_3WJ: 5′-GGA UCA AUC AUG GCA A-3′.

The therapeutic 3WJ-EGFRapt/anti-miR-21 is composed of four strands (Figure 2A). Lowercase letters indicate 2′-F modified nucleotides:

Strand 1: 5′-GGA ucA Auc AuG GcA A (C6-NH) (Alexa 647)-3′;

Strand 2: 5′-uuG ccA uGu GuA uGu GGG Auc ccG cGG ccA uGG cGG ccG GGA G-3′;

Strand 3: 5′-ccc AcA uAc uuu Guu GAu ccG ccu uAG uAA cGu Gcu uuG AuG ucG Auu cGA cAG GAG Gc-3′ (underlined sequence is EGFR aptamer⁵¹);

Strand 4: 5′-+G+A+T+A+A+G+C+T CTC CCG GCC GCC ATG GCC GCG GGA T-3′ (underlined sequence is 8-mer anti-miR21 LNA).

The RNA fragments were synthesized chemically (Trilink and Exiqon) and strands 1–3 are 2′-F modified at cytosine (C) and uracil (U) nucleotides to make the RNA nanoparticles resistant to RNase degradation. The pRNA-3WJ nanoparticles were assembled by mixing the four strands at equal molar concentrations in annealing buffer (10 mM Tris, pH 7.5–8.0, 50 mM NaCl, 1 mM EDTA), and heated to 95 °C for 5 min and slowly cooled to 4 °C over 45 min. Step-wise assembly of the RNA nanoparticles was verified on a native 10% PAGE running in 1× TBE (89 mM Tris-borate, 2 mM EDTA) buffer and imaged by Typhoon FLA 7000 (GE Healthcare) under Cy5 channel.

Characterization of the Assembled pRNA-3WJ Nanoparticle

The assembly of the functionalized 3WJ nanoparticles was characterized by native poly acrylamide gel electrophoresis (PAGE) assays followed by imaging by Typhoon FLA 7000 (GE Healthcare). The structures of the assembled 3WJ complexes were assessed by atomic force microscopy (AFM), and dynamic light scattering (DLS) as described previously.^38,76−78 RNA images (Figure 1C) were generated using specially modified mica surfaces (APS mica)⁷⁷ and imaged with a Veeco MultiMode AFM NanoScope IV system, operating in tapping mode. DLS measurement revealed that the size of the 3WJ core (Figure 1A) was 4.2 ± 1.1 nm (Figure 1D), and the size of the 3WJ-EGFRapt/ant-miR-21 nanoparticle (Figure 2A) was 14.8 ± 2.6 nm (Figure 2C). Because of the resolution limit of AFM image affected by the tip size of 4–10 nm, the size of 3WJ core and 3WJ-EGFRapt/ant-miR-21 nanoparticle were too small to reveal the detail structure and shape. To evaluate the global structures of the RNA nanoparticles derived from the 3WJ core, of which the crystal structure has been solved,⁴⁹ 58–60 bp of dsRNA was extended to the three arms of the 3WJ core (Figure 1B). It is expected that 58–60 bp is within the persistence length (stiffness) of 229 bp,⁷⁹ thus the AFM image of the resulting arm-extended RNA nanoparticle (Figure 1C) is expected to provide some information about the global structure of RNA nanoparticles derived from the thermodynamically stable 3WJ. Apparent hydrodynamic sizes and zeta potential of preassembled 3WJ (1.5 μM in DEPC H₂O) and 3WJ-EGFRapt/anti-miR-21 (1.35 μM in TBE buffer) were measured by Zetasizer nano-ZS (Malvern Instrument, LTD) at 25 °C, respectively. The data were obtained from three independent measurements.

Temperature Gradient Gel Electrophoresis (TGGE) Assay

The thermodynamic stability of the 2′-F modified 3WJ-EGFRapt/anti-miR-21 nanoparticles was studied using the TGGE system (Biometra GmbH, Germany). One of the fragments (c_3WJ) was 3′-end labeled with Alexa647 prior to the assembly of the complex. The 3WJ-EGFRapt/anti-miR-21 nanoparticles were subjected to a 10% native PAGE (2.5 uL of 1 μM RNA nanoparticles per well) and allowed to run in TBM buffer (89 mM Tris, 200 mM boric acid, and 2.5 mM MgCl₂) for 5 min at ambient temperature at constant 100 V. After running the RNA into the gel matrix, the gel was transferred into TGGE apparatus and a linear temperature gradient was set up from 25 to 80 °C perpendicular to the electrical current. The gel was run at 80 V for 90 min, and then imaged by Typhoon FLA 7000 (GE Healthcare). The intact particle fraction within the total RNA was analyzed by ImageJ, and the melting curve of the construct was fitted using nonlinear sigmoidal fitting. The apparent T_m of the pRNA-3WJ nanoparticle was determined as the temperature at which 50% of the RNA nanoparticle remained assembled.

Serum Stability Assay

The chemical stability of 3WJ-EGFRapt/anti-miR-21 nanoparticles was studied by incubating the RNA nanoparticle with 50% fetal bovine serum (FBS) at 37 °C at final concentration of 2 μM. 10 μL of samples were collected at each time point (0, 0.5, 1, 2, 4, 8, 12, 24, and 36 h) and were subjected to a 10% native PAGE assay with TBM running buffer. The gel was run at 120 V for 120 min, and then imaged by Typhoon FLA 7000 (GE Healthcare). The fraction of intact nanoparticle within the total RNA was analyzed and quantified by ImageJ.

Cell Culture

Human TNBC cell line MDA-MB-231 (American Type Culture Collection, ATCC) and MDA-MB-231-Luc (expressing luciferase reporter gene) were grown and cultured in DME/F-12 (1:1) medium (Thermo Scientific) containing 10% FBS inside a 37 °C incubator with 5% CO₂ and a humidified atmosphere.

In Vitro Binding Assay Using Fluorescence-Activated Cell Sorting (FACS)

MDA-MB-231 cells were trypsinized and rinsed with blank DME/F-12 (1:1) medium. 100 nM Alexa647 labeled 3WJ-EGFRapt/anti-miR-21 and the control RNA nanoparticles without EGFR aptamer were each incubated with 2 × 10⁵ MDA-MB-231 cells at 37 °C for 2 h. After washing with PBS (137 mM NaCl, 2.7 mM KCl, 100 mM Na₂HPO₄, 2 mM KH₂PO₄, pH 7.4), the cells were resuspended in PBS buffer. FACS was performed using Flow Cytometer (Becton, Dickinson) (available at the University of Kentucky Markey Cancer Center Flow Cytometry and Cell Sorting core facility) to observe the cell binding efficiency of the RNA nanoparticles. The data were analyzed by FlowJo 7.6.1 software.

In Vitro Binding and Internalization Assay Using Confocal Microscopy Imaging

MDA-MB-231 cells were grown on Lab-TekII 8-well chamber slide (Nunc) in DME/F-12 (1:1) medium overnight. 100 nM concentration of Alexa647 labeled 3WJ-EGFRapt/anti-miR-21 and the control 3WJ, 3WJ-EGFRapt, and 3WJ-anti-miR-21 were each incubated with the cells at 37 °C for 2 h. After washing with PBS, the cells were fixed by 4% paraformaldehyde (PFA) and washed 3 times by PBS. The cytoskeleton of the fixed cells was stained by Alexa488 Phalloidin (Life Technologies) for 30 min at room temperature and then rinsed with PBS for 3 × 10 min. The cells were mounted with Prolong Gold antifade reagent. DAPI (Life Technologies) was used for staining the nucleus. The cells were then assayed for nanoparticles binding and cellular entry by FluoView FV1000-Filter Confocal Microscope System (Olympus) (available at the University of Kentucky Markey Cancer Center imaging core facility).

Dual Luciferase Assay to Analyze Delivery of Anti-miR-21 by pRNA-3WJ Nanoparticles

MDA-MB-231 cells were grown on 24-well plates in DME/F-12 (1:1) medium until they reached 80% confluence. Cells were transfected with 150 ng psi-Check 2 plasmid (Promega) which contains an oncogenic miR-21 binding sequences at the 3′-UTR region of Renilla Luciferase gene using Lipofectamine 2000 (Life Technologies). Four hrs after transfection, the medium was replaced with complete DME/F-12 (1:1) medium and the cells were incubated for another 2 h. Various concentration of 3WJ-EGFRapt/anti-miR-21 and the control RNA 3WJ-anti-miR-21 (0, 10, 50, 250 nM) were then incubated with cells in opti-MEM at 37 °C for 2 h, respectively. After incubation with the RNA, complete DME/F-12 (1:1) medium was added into cells and Duel-luciferase assay (Promega) was used to evaluate the anti-miR-21 effects 24 h post-transfection following manufacturer’s instruction. Briefly, cells were washed once with PBS and lysed with passive lysis buffer. The cell culture plates were shaken for 15 min at room temperature. 20 μL of the lysate were added to 50 μL of luciferase assay reagent (LAR II) in 96-well plates and control firefly luciferase activity was measured. Upon addition of 50 μL of Stop & Glo Reagent, measurements of Renilla luciferase activity were then obtained. The Renilla luciferase activity was then normalized with respect to the firefly luciferase activity for determining the relative ratio of Renilla to firefly luciferase activity. At least three independent experiments were performed.

Assay the Effects of RNA Nanoparticles on TNBC Cells in Cell Cultures Using qRT-PCR

MDA-MB-231 cells were incubated with 100 nM of the 3WJ-EGFRapt/anti-miR-21 and control RNA nanoparticles, respectively. After 72 h treatment, cells were collected and target gene up-regulation effects were assessed by qRT-PCR at the mRNA level. Cells were processed for total RNA using Trizol RNA extraction reagent following manufacturer’s instruction (Life Technologies).

To assay the miR-21 down-regulation, the TaqMan microRNA Assays were performed according to manufacturer’s instruction (Life Technologies). Briefly, 10 ng total RNA was used to perform the reverse transcription using TaqMan MicroRNA Reverse Transcription Kit (Life Technologies). MiR-21 specific RT primers were used. Real-time PCR was performed using Taqman Assay. All reactions were carried out in a final volume of 20 μL using Taqman Universal PCR Master Mix and assayed in triplicates. Primers/probe set for human miR-21, and U6 (endogenous control) were purchased from Life Technologies. PCR was performed on StepOne/StepOnePlus systems (Applied Biosystems). The data was analyzed by the comparative CT Method (ΔΔCT Method).

To assay the downstream target genes (PTEN and PDCD4) of miR-21, TaqMan Gene Expression Assays was performed according to manufacturer’s instruction (Life Technologies). Briefly, the first cDNA strand was synthesized from total RNA (1 μg) using SuperScript III First-Strand Synthesis System (Life Technologies) from MDA-MB-231 cells with the various RNA nanoparticles treatment. Real-time PCR was performed using Taqman Assay. All reactions were carried out in a final volume of 20 μL using 2 × Taqman Fast Universal PCR Master Mix and assayed in triplicate. Primers/probe set for human PTEN, PDCD4, and 18S (endogenous control) were purchased from Life Technologies. PCR was performed on StepOne/StepOnePlus systems (Applied Biosystem). The data was analyzed by (ΔΔCT Method).

Apoptosis Studies in Cell Culture

In order to assay the cellular effects after RNA nanoparticle treatment, MDA-MB-231 cells were grown on 24-well plates in DME/F-12 (1:1) medium until they reached 80% confluence. Cells were then treated with 100 nM 3WJ-EGFRapt/anti-miR-21. The controls include anti-miR-21, 3WJ-anti-miR-21, and 3WJ-EGFRapt/Scramble. Twenty-four hours after incubation with the RNAs, the cellular Caspase-3 activity was measured and compared by Caspase-3 Assay Kit (BD Pharmingen) according to manufacturer’s instruction. Briefly, cell lysates (1–10 × 10⁶ cells/ml) after induction of apoptosis were prepared using cold cell lysis buffer provided by the kit, and incubated for 30 min on ice. For each sample, 50 μL of cell lysate was added with 5 μL reconstituted Ac-DEVD-AMC in HEPES buffer and incubated at 37 °C for 1 h. The amount of AMC liberated from Ac-DEVD-AMC was measured using an excitation wavelength of 380 nm and an emission wavelength range of 420–460 nm on Fluorolog fluorospectrometer (Horiba Jobin Yvon). Camptothecin (CPT) was used as a positive control, which was added into cell culture medium 4 h prior to the analysis of the caspase-3 activity.

Animal Models

All protocols involving animals are performed under the supervision of the University of Kentucky Institutional Animal Care and Use Committee (IACUC). To generate TNBC orthotropic model, female athymic nu/nu mice, 4–8 weeks old, were purchased from Taconic laboratories. Orthotopic tumor xenografts was established by directly injecting 2 × 10⁶ MDA-MB-231-Luc cells resuspended in PBS into the mammary fat pad of nude mice. When the tumor showed sign of growth, the mice were used for assaying therapeutic effects. When the tumor nodules had reached a volume of 100 mm³ approximately 15 days postinjection, the mice were used for tumor targeting studies.

Fluorescence Imaging to Detect the Binding of RNA Nanoparticles to Orthotopic TNBC Xenografts In Vivo

To investigate the delivery of pRNA-3WJ nanoparticles in vivo, a fluorescence imaging study was performed after tail vein injection of 100 μL 20 uM Alexa647 labeled 3WJ-EGFRapt/anti-miR-21 into orthotopic TNBC tumor mice. PBS injected mice were used as fluorescence negative control, The mice were sacrificed at 8 h post injection by inhalation of CO₂ followed by cervical dislocation, and major internal organs including heart, lungs, liver, spleen, kidneys together with tumor from the sacrificed mice were collected and subjected to fluorescence imaging for assessment of biodistribution profile using IVIS Spectrum station (Caliper Life Sciences) with excitation at 640 nm and emission at 680 nm. The tumors were further fixed in 4% PFA with 10% sucrose in PBS overnight at 4 °C and embedded in Tissue-Tek O.C.T. compound (Sakura Finetek USA, Inc.) for frozen sectioning (10 μm thick). The sectioned samples were mounted by ProLong Gold Antifade Reagent with DAPI (Life Technologies) overnight. The fluorescent images were obtained using FluoView FV1000-Filter Confocal Microscope System (Olympus) (available at the University of Kentucky Markey Cancer Center Imaging core facility).

Assay for the Therapeutics Effect of RNA Nanoparticles on Regression of TNBC Cells in Animal Models

When the tumor size reached about 5 mm in diameter, TNBC tumor bearing mice were randomly divided into two groups (N = 12 each group). One group was injected with RNA nanoparticle 3WJ-EGFRapt/anti-miR-21 through tail vein over the course of 5 injections (Total RNA nanoparticle dose: 5 mg/kg; LNA dose: 0.26 mg/kg) every other day. PBS treated mice were served as treatment control. The tumor volume was measured and monitored every 2 days. The tumor volume was calculated as (length × width²)/2. At the beginning and end of the injections, mice were subjected to bioluminescence whole body imaging to detect the endogenous luciferase expression level. Mice were anesthetized and intraperitoneally injected with 150 mg/kg d-luciferin (Biosynth International, Inc.). Bioluminescence from the anesthetized mice was detected by IVIS Spectrum station (Caliper Life Sciences). The mice were then sacrificed, tumors extracted and weighed followed by biochemical and histological analysis.

To quantify the miR-21 and subsequent target gene expression, the tumor tissues were flash frozen in liquid nitrogen and grounded using a mortar. The grounded tumor tissues were transferred to clean centrifuge tubes. Trizol RNA extraction reagent (Life Technologies) was added to extract the total RNA. Then, the miR-21 and subsequent target gene (PTEN and PDCD4) expression were quantified by qRT-PCR using Taqman Assays (Life Technologies) as described above.

Tumor tissues were also lysed in lysis buffer (2% SDS containing phosphatase and protease inhibitor cocktails) (Calbiochem) to quantify target gene expression at the protein level using Western blot assay. Protein concentration was measured by BCA Protein Assay kit (Pierce). Equal amount of total protein was subjected to SDS-PAGE gel electrophoresis and transferred from gel to membrane. Membranes were blocked by 5% fat-free milk at room temperature for 1 h and incubated overnight in primary antibody (PTEN, Cell Signaling, 1:1000; PDCD4, Cell Signaling, 1:1000; Lamin A/C, Santa Cruz, 1:1000). Protein bands were detected with an ECL system (Pierce) after incubating in the HRP-conjugated secondary antibody for 1 h at room temperature and exposed to film for autoradiography.

The therapeutic effects of RNA nanoparticles were evaluated by histological profiling of Ki67 and active caspase-3 activity in tumor tissues. Control and RNA nanoparticles treated group tumor sections were deparaffined by incubating with xylene (10 min once, for three times), and hydrated from 100% ethanol, 95% ethanol, 85% ethanol and 70% ethanol to PBS solution. Slides were then incubated with 3% H₂O₂ for 20 min to block endogenous peroxidase. At the antigen retrieval step, the slides were steamed in 10 mM citrate sodium buffer (pH 6.0) for 30 min. All slides were blocked by 5% goat serum and Avidin/Biotin Blocking Kit (Vector laboratories). Then slides were incubated with primary antibodies (Ki67, Spring Bioscience, 1:500; Active caspase-3, Millipore, 1:100) at 4 °C overnight, and then the sections were incubated with goat antirabbit IgG conjugated with HRP at room temperature for 60 min. The conjugated antibody was detected by diaminobenzidine (DAB). All slides were counterstained with Hematoxylin and images were taken by Nikon microscope.

Statistical Analysis

Each experiment was repeated at least 3 times with duplication for each samples tested. The results were presented as mean ± standard deviation, unless otherwise indicated. Statistical differences were evaluated using Student’s t test, and p < 0.05 was considered significant.