Between January 1996 and November 1998 38 patients were treated with induction chemotherapy. Pati... more Between January 1996 and November 1998 38 patients were treated with induction chemotherapy. Patients were distributed in two randomized groups, 19-19 patients in each, receiving (group N) either bleomycin, vincristine and methotrexate (BVM) or the previous medication plus cisplatin (BVCM) chemotherapy (group C). Side effects were low and reversible during the treatments. The clinical regression rate (RR) of the cases was 87% including complete regression 24%. There was no difference between the two groups. There was no difference in the pathological macroscopic regression, however group C was better in microscopic regression. During the 27.5 months of average follow-up time, group N had a significantly better result in the tumor-free survival, with a lower rate of metastatic recidives (N/C = 2/9). There was no significant difference in the overall survival rate, due to the radical neck dissections of recidive metastases. According to our experience we recommend the use of cisplatin...
The fibroepithelial polyp is a benign tumour that occurs as a rare intraluminal mass within the u... more The fibroepithelial polyp is a benign tumour that occurs as a rare intraluminal mass within the urinary tract. Most commonly it is located within the ureter or ureteropelvic junction. The authors present 3 cases of fibroepithelial polyp causing obstruction of the ureteropelvic junction. Dismembering pyelonplasty resulted in disappearance of the hydronephrosis, and the postoperative course was uneventful.
The authors performed a comparative lectin histochemical study on lymphoid organs of three specie... more The authors performed a comparative lectin histochemical study on lymphoid organs of three species with 12 different lectins. The T and B cell regions and the accessory cells of lymphoid tissues were studied. The species differences show that the BALB/c mouse can be a valuable tool in modelling human lymphoid tissues, whereas Wistar rats exhibit great differences in lectin binding patterns as compared with human tissues. The positivity of peanut agglutinin binding of cortical and the negativity of medullar thymocytes in mice can be regarded as an indicator of cell differentiation. The focal distribution of peanut agglutinin positivity in the centrocytic region of human and rat germinal centres marks a small B cell subpopulation at the early stage of differentiation, while Bandeira simplicifolia binding is characteristic of a broader B cell lineage, but only in rats and mice. Canavalia ensiformis lectin seems to be a reliable marker of accessory cells (dendritic and interdigitating r...
BACKGROUND Chronic rhinosinusitis (CRS) is a multifactorial upper airway disease with unclear eti... more BACKGROUND Chronic rhinosinusitis (CRS) is a multifactorial upper airway disease with unclear etiology. Neuronal Transient Receptor Potential Vanilloid 1 (TRPV1) and Ankyrin 1 (TRPA1) channels have been implicated in the pathogenesis of CRS. We aimed to detect the expression of extraneuronal TRPV1 and TRPA1 receptors in nasal polyp (NP) tissue samples. METHODOLOGY Samples were obtained from fourty-two CRS pateints with nasal polyp and sixteen healthy controls to measure receptor gene expression by quantitative PCR, protein localization by immunohistochemistry and cytokine profile by multiplex bead immunoassay. RESULTS Non-neuronal TRPV1, TRPA1 receptors were expressed in biopsy samples of NP. A population of mast cells and macrophages were immunopositive for TRPV1 and TRPA1. A fraction of plasma cells expressed TRPV1 but not TRPA1 and neither receptor was present on eosinophils. The local gene expression of extraneuronal TRPV1, TRPA1 receptors was also proven. TRPV1 mRNA levels were significantly increased in CRSwNP patients with asthma and allergic rhinitis compared to their NP counterparts. CONCLUSIONS Elevated TRPV1 levels in comorbid asthma and allergy may have a function in CRSwNP. Subpopulation-specific TRPV1 presence on plasma and mast cells can indicate delicate roles in regulating activation and release of inflammatory mediators.
The highly variable biological behaviour of neuroblastoma, a neoplasm which belongs to the family... more The highly variable biological behaviour of neuroblastoma, a neoplasm which belongs to the family of primitive neuroectodermal tumours, is determined by its molecular pathological characteristics. Among them amplification of the N-myc gene is the most important factor in both therapeutic and prognostic points of view. Value of the amplification can be determined by different methods. The latest of them is the competitive polymerase chain reaction (PCR), essence of which is the parallel reaction of the target N-myc gene (exon 3.) and the endogen competitor CF gene (exon 3.) in the same reaction solution. The authors applied the method on 11 neuroblastoma cases diagnosed between 1994 and january of 1999. In three cases the amplification was determined also by fluorescens in situ hybridization (FISH). Six of the 11 cases were detected to have more than 10-fold, two of the six about 100-fold N-myc amplification. Results of the PCR and FISH correlated well. The two methods applied by the...
An interesting multiple tumor case is described in which 4 different kinds of tumors were diagnos... more An interesting multiple tumor case is described in which 4 different kinds of tumors were diagnosed in the same patient at autopsy and histopathologic examination. The tumors were the following: 1) prolactinoma of the anterior pituitary lobe; 2) basal cell carcinoma of the nose; 3) adenocarcinoma of the colon sigmoideum; 4) multiple oncocytomas (oncocytomatosis) in the kidneys. Immunohistochemical investigation for p53 revealed a strong intranuclear positivity in the colonic carcinoma cells as a result of the overexpression of a possible mutant type of the protein. The other 3 tumors were negative with the p53-specific DO-7 antibody, therefore, no point mutation was thought to be present in the p53 gene of the tumor cells. The immunohistochemical and anamnestic data suggested that this is not a hereditary syndrome, and there is no common pathogenesis of these tumors. Its rarity is interesting in our case because of the coincidence of 4 different unrelated tumors and the absence of a...
The authors performed a comparative lectin histochemical study on lymphoid organs of three specie... more The authors performed a comparative lectin histochemical study on lymphoid organs of three species with 12 different lectins. The T and B cell regions and the accessory cells of lymphoid tissues were studied. The species differences show that the BALB/c mouse can be a valuable tool in modelling human lymphoid tissues, whereas Wistar rats exhibit great differences in lectin binding patterns as compared with human tissues. The positivity of peanut agglutinin binding of cortical and the negativity of medullar thymocytes in mice can be regarded as an indicator of cell differentiation. The focal distribution of peanut agglutinin positivity in the centrocytic region of human and rat germinal centres marks a small B cell subpopulation at the early stage of differentiation, while Bandeira simplicifolia binding is characteristic of a broader B cell lineage, but only in rats and mice. Canavalia ensiformis lectin seems to be a reliable marker of accessory cells (dendritic and interdigitating r...
Between January 1996 and November 1998 38 patients were treated with induction chemotherapy. Pati... more Between January 1996 and November 1998 38 patients were treated with induction chemotherapy. Patients were distributed in two randomized groups, 19-19 patients in each, receiving (group N) either bleomycin, vincristine and methotrexate (BVM) or the previous medication plus cisplatin (BVCM) chemotherapy (group C). Side effects were low and reversible during the treatments. The clinical regression rate (RR) of the cases was 87% including complete regression 24%. There was no difference between the two groups. There was no difference in the pathological macroscopic regression, however group C was better in microscopic regression. During the 27.5 months of average follow-up time, group N had a significantly better result in the tumor-free survival, with a lower rate of metastatic recidives (N/C = 2/9). There was no significant difference in the overall survival rate, due to the radical neck dissections of recidive metastases. According to our experience we recommend the use of cisplatin...
The highly variable biological behaviour of neuroblastoma, a neoplasm which belongs to the family... more The highly variable biological behaviour of neuroblastoma, a neoplasm which belongs to the family of primitive neuroectodermal tumours, is determined by its molecular pathological characteristics. Among them amplification of the N-myc gene is the most important factor in both therapeutic and prognostic points of view. Value of the amplification can be determined by different methods. The latest of them is the competitive polymerase chain reaction (PCR), essence of which is the parallel reaction of the target N-myc gene (exon 3.) and the endogen competitor CF gene (exon 3.) in the same reaction solution. The authors applied the method on 11 neuroblastoma cases diagnosed between 1994 and january of 1999. In three cases the amplification was determined also by fluorescens in situ hybridization (FISH). Six of the 11 cases were detected to have more than 10-fold, two of the six about 100-fold N-myc amplification. Results of the PCR and FISH correlated well. The two methods applied by the...
The fibroepithelial polyp is a benign tumour that occurs as a rare intraluminal mass within the u... more The fibroepithelial polyp is a benign tumour that occurs as a rare intraluminal mass within the urinary tract. Most commonly it is located within the ureter or ureteropelvic junction. The authors present 3 cases of fibroepithelial polyp causing obstruction of the ureteropelvic junction. Dismembering pyelonplasty resulted in disappearance of the hydronephrosis, and the postoperative course was uneventful.
Four alveolar soft-part sarcomas were investigated by means of standard immunohistochemistry and ... more Four alveolar soft-part sarcomas were investigated by means of standard immunohistochemistry and interphase cytogenetics to further characterize the immunophenotype and proliferative activity of this tumor. The main goal of this study was to explore the chromosomal changes of this rare soft-tissue sarcoma. One epithelial (KLI), three neurogenic [neuron specific enolase (NSE), PGP 9.5, and S100], and five myogenic (desmin, myoglobin, alpha-smooth mnuscle actin, alpha-sarcomeric actin, and MyoD1) markers were used for the immunophenotypical analysis. Proliferative activity was assessed using the Ki67 index. Twelve (peri)centromeric (1, 3, 4, 6, 7, 8, 10, 12, 15, 17, 18, and X) and one telomeric (17q25-qtel.) chromosomal probes were used for interphase cytogenetic analysis. Three of the cases showed cytoplasmic desmin and/or myoglobin, and one showed smooth muscle actin positivity. All of the four tumors had granular, cytoplasmic, possibly nonspecific MyoD1 and sarcomeric actin positivity. Two of the tumors were positive for vimentin, four gave focal and weak staining with neurogenic markers (four of four NSE, one of four S100, and four of four PGP 9.5), but none of them was positive with KLI. Alveolar soft-part sarcomas may show myogenic immunophenotype in a number of cases, which supports myogenic differentiation. Fluorescent in situ hybridization using alpha satellite chromosomal probes revealed significant alterations in all of the cases. Most frequent and repeated numerical changes, which seem to be characteristic of the neoplasm and may play an important part in its pathogenesis and/or progression, were trisomy 7, monosomy 8 and monosomy 18.
Between January 1996 and November 1998 38 patients were treated with induction chemotherapy. Pati... more Between January 1996 and November 1998 38 patients were treated with induction chemotherapy. Patients were distributed in two randomized groups, 19-19 patients in each, receiving (group N) either bleomycin, vincristine and methotrexate (BVM) or the previous medication plus cisplatin (BVCM) chemotherapy (group C). Side effects were low and reversible during the treatments. The clinical regression rate (RR) of the cases was 87% including complete regression 24%. There was no difference between the two groups. There was no difference in the pathological macroscopic regression, however group C was better in microscopic regression. During the 27.5 months of average follow-up time, group N had a significantly better result in the tumor-free survival, with a lower rate of metastatic recidives (N/C = 2/9). There was no significant difference in the overall survival rate, due to the radical neck dissections of recidive metastases. According to our experience we recommend the use of cisplatin...
The fibroepithelial polyp is a benign tumour that occurs as a rare intraluminal mass within the u... more The fibroepithelial polyp is a benign tumour that occurs as a rare intraluminal mass within the urinary tract. Most commonly it is located within the ureter or ureteropelvic junction. The authors present 3 cases of fibroepithelial polyp causing obstruction of the ureteropelvic junction. Dismembering pyelonplasty resulted in disappearance of the hydronephrosis, and the postoperative course was uneventful.
The authors performed a comparative lectin histochemical study on lymphoid organs of three specie... more The authors performed a comparative lectin histochemical study on lymphoid organs of three species with 12 different lectins. The T and B cell regions and the accessory cells of lymphoid tissues were studied. The species differences show that the BALB/c mouse can be a valuable tool in modelling human lymphoid tissues, whereas Wistar rats exhibit great differences in lectin binding patterns as compared with human tissues. The positivity of peanut agglutinin binding of cortical and the negativity of medullar thymocytes in mice can be regarded as an indicator of cell differentiation. The focal distribution of peanut agglutinin positivity in the centrocytic region of human and rat germinal centres marks a small B cell subpopulation at the early stage of differentiation, while Bandeira simplicifolia binding is characteristic of a broader B cell lineage, but only in rats and mice. Canavalia ensiformis lectin seems to be a reliable marker of accessory cells (dendritic and interdigitating r...
BACKGROUND Chronic rhinosinusitis (CRS) is a multifactorial upper airway disease with unclear eti... more BACKGROUND Chronic rhinosinusitis (CRS) is a multifactorial upper airway disease with unclear etiology. Neuronal Transient Receptor Potential Vanilloid 1 (TRPV1) and Ankyrin 1 (TRPA1) channels have been implicated in the pathogenesis of CRS. We aimed to detect the expression of extraneuronal TRPV1 and TRPA1 receptors in nasal polyp (NP) tissue samples. METHODOLOGY Samples were obtained from fourty-two CRS pateints with nasal polyp and sixteen healthy controls to measure receptor gene expression by quantitative PCR, protein localization by immunohistochemistry and cytokine profile by multiplex bead immunoassay. RESULTS Non-neuronal TRPV1, TRPA1 receptors were expressed in biopsy samples of NP. A population of mast cells and macrophages were immunopositive for TRPV1 and TRPA1. A fraction of plasma cells expressed TRPV1 but not TRPA1 and neither receptor was present on eosinophils. The local gene expression of extraneuronal TRPV1, TRPA1 receptors was also proven. TRPV1 mRNA levels were significantly increased in CRSwNP patients with asthma and allergic rhinitis compared to their NP counterparts. CONCLUSIONS Elevated TRPV1 levels in comorbid asthma and allergy may have a function in CRSwNP. Subpopulation-specific TRPV1 presence on plasma and mast cells can indicate delicate roles in regulating activation and release of inflammatory mediators.
The highly variable biological behaviour of neuroblastoma, a neoplasm which belongs to the family... more The highly variable biological behaviour of neuroblastoma, a neoplasm which belongs to the family of primitive neuroectodermal tumours, is determined by its molecular pathological characteristics. Among them amplification of the N-myc gene is the most important factor in both therapeutic and prognostic points of view. Value of the amplification can be determined by different methods. The latest of them is the competitive polymerase chain reaction (PCR), essence of which is the parallel reaction of the target N-myc gene (exon 3.) and the endogen competitor CF gene (exon 3.) in the same reaction solution. The authors applied the method on 11 neuroblastoma cases diagnosed between 1994 and january of 1999. In three cases the amplification was determined also by fluorescens in situ hybridization (FISH). Six of the 11 cases were detected to have more than 10-fold, two of the six about 100-fold N-myc amplification. Results of the PCR and FISH correlated well. The two methods applied by the...
An interesting multiple tumor case is described in which 4 different kinds of tumors were diagnos... more An interesting multiple tumor case is described in which 4 different kinds of tumors were diagnosed in the same patient at autopsy and histopathologic examination. The tumors were the following: 1) prolactinoma of the anterior pituitary lobe; 2) basal cell carcinoma of the nose; 3) adenocarcinoma of the colon sigmoideum; 4) multiple oncocytomas (oncocytomatosis) in the kidneys. Immunohistochemical investigation for p53 revealed a strong intranuclear positivity in the colonic carcinoma cells as a result of the overexpression of a possible mutant type of the protein. The other 3 tumors were negative with the p53-specific DO-7 antibody, therefore, no point mutation was thought to be present in the p53 gene of the tumor cells. The immunohistochemical and anamnestic data suggested that this is not a hereditary syndrome, and there is no common pathogenesis of these tumors. Its rarity is interesting in our case because of the coincidence of 4 different unrelated tumors and the absence of a...
The authors performed a comparative lectin histochemical study on lymphoid organs of three specie... more The authors performed a comparative lectin histochemical study on lymphoid organs of three species with 12 different lectins. The T and B cell regions and the accessory cells of lymphoid tissues were studied. The species differences show that the BALB/c mouse can be a valuable tool in modelling human lymphoid tissues, whereas Wistar rats exhibit great differences in lectin binding patterns as compared with human tissues. The positivity of peanut agglutinin binding of cortical and the negativity of medullar thymocytes in mice can be regarded as an indicator of cell differentiation. The focal distribution of peanut agglutinin positivity in the centrocytic region of human and rat germinal centres marks a small B cell subpopulation at the early stage of differentiation, while Bandeira simplicifolia binding is characteristic of a broader B cell lineage, but only in rats and mice. Canavalia ensiformis lectin seems to be a reliable marker of accessory cells (dendritic and interdigitating r...
Between January 1996 and November 1998 38 patients were treated with induction chemotherapy. Pati... more Between January 1996 and November 1998 38 patients were treated with induction chemotherapy. Patients were distributed in two randomized groups, 19-19 patients in each, receiving (group N) either bleomycin, vincristine and methotrexate (BVM) or the previous medication plus cisplatin (BVCM) chemotherapy (group C). Side effects were low and reversible during the treatments. The clinical regression rate (RR) of the cases was 87% including complete regression 24%. There was no difference between the two groups. There was no difference in the pathological macroscopic regression, however group C was better in microscopic regression. During the 27.5 months of average follow-up time, group N had a significantly better result in the tumor-free survival, with a lower rate of metastatic recidives (N/C = 2/9). There was no significant difference in the overall survival rate, due to the radical neck dissections of recidive metastases. According to our experience we recommend the use of cisplatin...
The highly variable biological behaviour of neuroblastoma, a neoplasm which belongs to the family... more The highly variable biological behaviour of neuroblastoma, a neoplasm which belongs to the family of primitive neuroectodermal tumours, is determined by its molecular pathological characteristics. Among them amplification of the N-myc gene is the most important factor in both therapeutic and prognostic points of view. Value of the amplification can be determined by different methods. The latest of them is the competitive polymerase chain reaction (PCR), essence of which is the parallel reaction of the target N-myc gene (exon 3.) and the endogen competitor CF gene (exon 3.) in the same reaction solution. The authors applied the method on 11 neuroblastoma cases diagnosed between 1994 and january of 1999. In three cases the amplification was determined also by fluorescens in situ hybridization (FISH). Six of the 11 cases were detected to have more than 10-fold, two of the six about 100-fold N-myc amplification. Results of the PCR and FISH correlated well. The two methods applied by the...
The fibroepithelial polyp is a benign tumour that occurs as a rare intraluminal mass within the u... more The fibroepithelial polyp is a benign tumour that occurs as a rare intraluminal mass within the urinary tract. Most commonly it is located within the ureter or ureteropelvic junction. The authors present 3 cases of fibroepithelial polyp causing obstruction of the ureteropelvic junction. Dismembering pyelonplasty resulted in disappearance of the hydronephrosis, and the postoperative course was uneventful.
Four alveolar soft-part sarcomas were investigated by means of standard immunohistochemistry and ... more Four alveolar soft-part sarcomas were investigated by means of standard immunohistochemistry and interphase cytogenetics to further characterize the immunophenotype and proliferative activity of this tumor. The main goal of this study was to explore the chromosomal changes of this rare soft-tissue sarcoma. One epithelial (KLI), three neurogenic [neuron specific enolase (NSE), PGP 9.5, and S100], and five myogenic (desmin, myoglobin, alpha-smooth mnuscle actin, alpha-sarcomeric actin, and MyoD1) markers were used for the immunophenotypical analysis. Proliferative activity was assessed using the Ki67 index. Twelve (peri)centromeric (1, 3, 4, 6, 7, 8, 10, 12, 15, 17, 18, and X) and one telomeric (17q25-qtel.) chromosomal probes were used for interphase cytogenetic analysis. Three of the cases showed cytoplasmic desmin and/or myoglobin, and one showed smooth muscle actin positivity. All of the four tumors had granular, cytoplasmic, possibly nonspecific MyoD1 and sarcomeric actin positivity. Two of the tumors were positive for vimentin, four gave focal and weak staining with neurogenic markers (four of four NSE, one of four S100, and four of four PGP 9.5), but none of them was positive with KLI. Alveolar soft-part sarcomas may show myogenic immunophenotype in a number of cases, which supports myogenic differentiation. Fluorescent in situ hybridization using alpha satellite chromosomal probes revealed significant alterations in all of the cases. Most frequent and repeated numerical changes, which seem to be characteristic of the neoplasm and may play an important part in its pathogenesis and/or progression, were trisomy 7, monosomy 8 and monosomy 18.
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Papers by T. Tornóczky