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WO2007133674A3 - Lentiviral vector compositions, methods and applications - Google Patents

Lentiviral vector compositions, methods and applications Download PDF

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Publication number
WO2007133674A3
WO2007133674A3 PCT/US2007/011385 US2007011385W WO2007133674A3 WO 2007133674 A3 WO2007133674 A3 WO 2007133674A3 US 2007011385 W US2007011385 W US 2007011385W WO 2007133674 A3 WO2007133674 A3 WO 2007133674A3
Authority
WO
WIPO (PCT)
Prior art keywords
vectors
lentiviral
expression
applications
lentiviral vector
Prior art date
Application number
PCT/US2007/011385
Other languages
French (fr)
Other versions
WO2007133674A2 (en
Inventor
Boro Dropulic
Yung Nien Chang
Original Assignee
Lentigen Corp
Boro Dropulic
Yung Nien Chang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lentigen Corp, Boro Dropulic, Yung Nien Chang filed Critical Lentigen Corp
Publication of WO2007133674A2 publication Critical patent/WO2007133674A2/en
Publication of WO2007133674A3 publication Critical patent/WO2007133674A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention provides vectors which allow efficient expression of genes or RNAs of interest (GROI) which possess internal splicing sites, including the construction of lentiviral vectors where expression of such GROIs do not result a truncated genomic lentiviral vector RNA. Moreover, the lentiviral vector constructs are designed such that expression from the vector can be modulated via inducible promoters. These lentiviral vectors, and in particular HIV vectors, are useful in functional genomics, drug discovery, target validation, protein production, vaccine production, clinical gene therapy, and other applications.
PCT/US2007/011385 2006-05-12 2007-05-11 Lentiviral vector compositions, methods and applications WO2007133674A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US79990306P 2006-05-12 2006-05-12
US60/799,903 2006-05-12
US60/816,426 2006-06-21
US81642606P 2006-06-26 2006-06-26
US83176406P 2006-07-18 2006-07-18
US60/831,764 2006-07-18

Publications (2)

Publication Number Publication Date
WO2007133674A2 WO2007133674A2 (en) 2007-11-22
WO2007133674A3 true WO2007133674A3 (en) 2008-02-28

Family

ID=38694493

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/011385 WO2007133674A2 (en) 2006-05-12 2007-05-11 Lentiviral vector compositions, methods and applications

Country Status (1)

Country Link
WO (1) WO2007133674A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010045659A1 (en) 2008-10-17 2010-04-22 American Gene Technologies International Inc. Safe lentiviral vectors for targeted delivery of multiple therapeutic molecules
EP3307894A4 (en) * 2015-06-10 2019-01-16 American Gene Technologies International, Inc. Non-integrating viral delivery system and methods of use thereof
US11980663B2 (en) 2015-07-08 2024-05-14 American Gene Technologies International Inc. HIV pre-immunization and immunotherapy
DK3402483T3 (en) 2016-01-15 2024-01-02 American Gene Tech Int Inc Methods and compositions for activating gamma-delta T cells
US10137144B2 (en) 2016-01-15 2018-11-27 American Gene Technologies International Inc. Methods and compositions for the activation of gamma-delta T-cells
US10888613B2 (en) 2016-02-08 2021-01-12 American Gene Technologies International Inc. Method of producing cells resistant to HIV infection
ES2911448T3 (en) 2016-03-09 2022-05-19 American Gene Tech Int Inc Combined Vectors and Methods for Cancer Treatment
JP7173548B2 (en) 2016-06-08 2022-11-16 アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド Non-Integrating Viral Delivery Systems and Related Methods
AU2017292582C1 (en) 2016-07-08 2021-11-11 American Gene Technologies International Inc. HIV pre-immunization and immunotherapy
EP3487507A4 (en) 2016-07-21 2020-04-08 American Gene Technologies International, Inc. Viral vectors for treating parkinson's disease
EP3607072A4 (en) 2017-04-03 2021-01-06 American Gene Technologies International Inc. Compositions and methods for treating phenylketonuria
GB201706121D0 (en) * 2017-04-18 2017-05-31 Glaxosmithkline Ip Dev Ltd Stable cell lines for retroviral production
CN114181970B (en) * 2020-09-15 2023-07-25 上海药明巨诺生物医药研发有限公司 Lentiviral vector purification method
CN112501210B (en) * 2020-12-07 2022-08-05 和元生物技术（上海）股份有限公司 Exogenous gene controllable expression slow virus packaging method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JOHANSEN ET AL.: "Evaluation of Tet-on system to avoid transgene down-regulation in ex vivo gene transfer to the CNS", GENE THER., vol. 9, no. 19, October 2002 (2002-10-01), pages 1291 - 1301, XP002458353, DOI: doi:10.1038/sj.gt.3301778 *

Also Published As

Publication number Publication date
WO2007133674A2 (en) 2007-11-22

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