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WO2000037671A3 - Improved in vivo production of cephalosporins - Google Patents

Improved in vivo production of cephalosporins Download PDF

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Publication number
WO2000037671A3
WO2000037671A3 PCT/EP1999/010292 EP9910292W WO0037671A3 WO 2000037671 A3 WO2000037671 A3 WO 2000037671A3 EP 9910292 W EP9910292 W EP 9910292W WO 0037671 A3 WO0037671 A3 WO 0037671A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide sequence
aca
acylated
side chain
produced
Prior art date
Application number
PCT/EP1999/010292
Other languages
French (fr)
Other versions
WO2000037671A2 (en
Inventor
Roelof Ary Lans Bovenberg
Richard Kerkman
Eric Koenhen
Original Assignee
Dsm Nv
Roelof Ary Lans Bovenberg
Richard Kerkman
Eric Koenhen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Nv, Roelof Ary Lans Bovenberg, Richard Kerkman, Eric Koenhen filed Critical Dsm Nv
Priority to AU30426/00A priority Critical patent/AU3042600A/en
Priority to JP2000589724A priority patent/JP2002533092A/en
Priority to EP99964657A priority patent/EP1141372A2/en
Priority to KR1020017007915A priority patent/KR20010089672A/en
Publication of WO2000037671A2 publication Critical patent/WO2000037671A2/en
Publication of WO2000037671A3 publication Critical patent/WO2000037671A3/en
Priority to HK02103286.5A priority patent/HK1041610A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention discloses a process for the production of 7-amino cephalosporanic acid (7-ACA) or a derivative thereof comprising the steps of fermenting a P. chrysogenum strain being transformed with an expression construct comprising a nucleotide sequence encoding expandase, hydroxylase and acetyltransferase activity in the presence of a suitable acyl side chain precursor, or a salt or ester thereof, such that an N-acylated 7-ACA compound is produced, N-deacylating the thus produced N-acylated 7-ACA compound and, optionally, acylating the free amino group and/or substituting the 3' acetate group with a side chain suitable to form a cephalosporin antibiotic, characterised in that the nucleotide sequence encoding the acetyltransferase is derived from Acremonium chrysogenum and starts at the second ATG of the open reading frame as present in said nucleotide sequence.
PCT/EP1999/010292 1998-12-22 1999-12-21 Improved in vivo production of cephalosporins WO2000037671A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU30426/00A AU3042600A (en) 1998-12-22 1999-12-21 Improved (in vivo) production of cephalosporins
JP2000589724A JP2002533092A (en) 1998-12-22 1999-12-21 Improved in vivo production of cephalosporins
EP99964657A EP1141372A2 (en) 1998-12-22 1999-12-21 Improved in vivo production of cephalosporins
KR1020017007915A KR20010089672A (en) 1998-12-22 1999-12-21 Improved in vivo production of cephalosporins
HK02103286.5A HK1041610A1 (en) 1998-12-22 2002-05-02 Improved in vivo production of cephalosporins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98204469 1998-12-22
EP98204469.5 1998-12-22

Publications (2)

Publication Number Publication Date
WO2000037671A2 WO2000037671A2 (en) 2000-06-29
WO2000037671A3 true WO2000037671A3 (en) 2000-09-14

Family

ID=8234568

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/010292 WO2000037671A2 (en) 1998-12-22 1999-12-21 Improved in vivo production of cephalosporins

Country Status (7)

Country Link
EP (1) EP1141372A2 (en)
JP (1) JP2002533092A (en)
KR (1) KR20010089672A (en)
CN (1) CN1331751A (en)
AU (1) AU3042600A (en)
HK (1) HK1041610A1 (en)
WO (1) WO2000037671A2 (en)

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Patent Citations (5)

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Title
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Also Published As

Publication number Publication date
CN1331751A (en) 2002-01-16
HK1041610A1 (en) 2002-07-12
WO2000037671A2 (en) 2000-06-29
EP1141372A2 (en) 2001-10-10
KR20010089672A (en) 2001-10-08
AU3042600A (en) 2000-07-12
JP2002533092A (en) 2002-10-08

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