WO2000068434A3 - Multiple tag analysis - Google Patents
Multiple tag analysis Download PDFInfo
- Publication number
- WO2000068434A3 WO2000068434A3 PCT/US2000/012391 US0012391W WO0068434A3 WO 2000068434 A3 WO2000068434 A3 WO 2000068434A3 US 0012391 W US0012391 W US 0012391W WO 0068434 A3 WO0068434 A3 WO 0068434A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- target
- molecules
- reporter molecules
- decoding
- detection
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Disclosed is a method of detecting multiple analytes in a sample in a single assay. The method is based on encoding target molecules with signals followed by decoding of the encoded signal. This encoding/decoding uncouples the detection of a target molecule from the chemical and physical properties of the target molecule. In basic form, the disclosed method involves association of one or more reporter molecules with one or more target samples, association of one or more decoding tags with the reporter molecules, and detection of the decoding tags. The reporter molecules associate with target molecules in the target sample(s). Generally, the reporter molecules correspond to one or more target molecules, and the decoding tags correspond to one or more reporter molecules. Thus, detection of particular decoding tags indicates the presence of the corresponding reporter molecules. In turn, the presence of particular reporter molecules indicates the presence of the corresponding target molecules. The sensitivity of the disclosed method can also be enhanced by including a signal amplification step prior to detection. Medical applications of this method include the analysis of the phenotypic status or replicative status of cells (growth or quiescence) and the assessment of normal and neoplastic cells in histologic or cytologic specimens in normal and disease states. For example, a pathologist may use the method to link a phenotypic state with the protein profile of lesion believed to contain malignant or pre-malignant cells.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP00930427A EP1196632A2 (en) | 1999-05-07 | 2000-05-05 | Multiple tag analysis |
| AU48248/00A AU782408B2 (en) | 1999-05-07 | 2000-05-05 | Multiple tag analysis |
| JP2000617205A JP2002543813A (en) | 1999-05-07 | 2000-05-05 | Multiple label analysis |
| CA002371843A CA2371843A1 (en) | 1999-05-07 | 2000-05-05 | Multiple tag analysis |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13296999P | 1999-05-07 | 1999-05-07 | |
| US60/132,969 | 1999-05-07 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2000068434A2 WO2000068434A2 (en) | 2000-11-16 |
| WO2000068434A3 true WO2000068434A3 (en) | 2002-01-31 |
Family
ID=22456431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2000/012391 WO2000068434A2 (en) | 1999-05-07 | 2000-05-05 | Multiple tag analysis |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1196632A2 (en) |
| JP (1) | JP2002543813A (en) |
| AU (1) | AU782408B2 (en) |
| CA (1) | CA2371843A1 (en) |
| WO (1) | WO2000068434A2 (en) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020106648A1 (en) * | 2000-05-05 | 2002-08-08 | Lizardi Paul M. | Highly multiplexed reporter carrier systems |
| US20020142345A1 (en) * | 2000-12-22 | 2002-10-03 | Nelsen Anita J. | Methods for encoding and decoding complex mixtures in arrayed assays |
| US7083951B2 (en) | 2001-02-14 | 2006-08-01 | The Regents Of The University Of California | Flow cytometric detection method for DNA samples |
| US20030124595A1 (en) * | 2001-11-06 | 2003-07-03 | Lizardi Paul M. | Sensitive coded detection systems |
| US7192703B2 (en) * | 2003-02-14 | 2007-03-20 | Intel Corporation, Inc. | Biomolecule analysis by rolling circle amplification and SERS detection |
| CA2611266C (en) | 2005-06-07 | 2015-11-24 | Centre National De La Recherche Scientifique (Cnrs) | Use of conjugates with linkers cleavable by photodissociation or fragmentation for mass spectrometry analysis of tissue sections |
| US20100105145A1 (en) * | 2005-07-01 | 2010-04-29 | Dako Denmark A/S | Method of simultaneously visualizing multiple biological targets |
| US8546309B2 (en) * | 2005-07-01 | 2013-10-01 | Dako Denmark A/S | Monomeric and polymeric linkers useful for conjugating biological molecules and other substances |
| WO2010114772A2 (en) * | 2009-04-03 | 2010-10-07 | Battelle Memorial Institute | Biological and chemical collection and detection |
| GB0908633D0 (en) * | 2009-05-20 | 2009-06-24 | D3 Technologies Ltd | Detection of target analytie by signal amplication |
| CN102667473B (en) | 2009-10-12 | 2016-06-08 | 文塔纳医疗系统公司 | Multi-modal contrast and bright field background rendering for enhanced pathology determination and multi-analyte detection of tissue |
| US10385392B2 (en) * | 2011-12-30 | 2019-08-20 | Abbott Molecular Inc. | Nucleic acid hybridization probes |
| CN105705938B (en) * | 2013-08-19 | 2020-11-06 | 休斯敦大学系统 | Phosphorescent reporters |
| EP3043891B1 (en) | 2013-09-13 | 2019-01-16 | The Board of Trustees of The Leland Stanford Junior University | Multiplexed imaging of tissues using mass tags and secondary ion mass spectrometry |
| CN106233421A (en) | 2014-04-02 | 2016-12-14 | 斯坦福大学托管董事会 | For being carried out equipment and the method for submicron element image analysis by mass spectrograph |
| US10036066B2 (en) | 2015-03-17 | 2018-07-31 | Dako Denmark A/S | In situ hybridization detection method |
| US9766224B2 (en) | 2015-03-25 | 2017-09-19 | The Board Of Trustees Of The Leland Stanford Junior University | Single cell analysis using secondary ion mass spectrometry |
| US9797879B2 (en) | 2015-04-23 | 2017-10-24 | The Board Of Trustees Of The Leland Stanford Junior University | Method for multiplexed sample analysis by photoionizing secondary sputtered neutrals |
| US20180164308A1 (en) | 2016-12-09 | 2018-06-14 | Ultivue, Inc. | Methods for multiplex imaging using labeled nucleic acid imaging agents |
| WO2018183876A1 (en) | 2017-03-31 | 2018-10-04 | Ultivue, Inc. | Dna-antigen exchange and amplification |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992002528A1 (en) * | 1990-07-27 | 1992-02-20 | Chiron Corporation | Incorporation of selectably clevable and/or abasic sites into oligonucleotide chains and reagents therefor |
| WO1992002526A1 (en) * | 1990-07-27 | 1992-02-20 | Chiron Corporation | Large comb-type branched polynucleotides |
| WO1995020320A1 (en) * | 1994-01-31 | 1995-08-03 | Trustees Of Boston University | Self-assembling multimeric nucleic acid constructs |
| US5614362A (en) * | 1987-10-15 | 1997-03-25 | Chiron Corporation | Nucleic acid hybridization assay for hepatitis B virus DNA |
| WO1998004746A1 (en) * | 1996-07-31 | 1998-02-05 | Mount Sinai School Of Medicine Of The City University Of New York | Nucleic acid amplification method: ramification-extension amplification method (ram) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6203989B1 (en) * | 1998-09-30 | 2001-03-20 | Affymetrix, Inc. | Methods and compositions for amplifying detectable signals in specific binding assays |
-
2000
- 2000-05-05 CA CA002371843A patent/CA2371843A1/en not_active Abandoned
- 2000-05-05 EP EP00930427A patent/EP1196632A2/en not_active Withdrawn
- 2000-05-05 AU AU48248/00A patent/AU782408B2/en not_active Ceased
- 2000-05-05 WO PCT/US2000/012391 patent/WO2000068434A2/en not_active Application Discontinuation
- 2000-05-05 JP JP2000617205A patent/JP2002543813A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5614362A (en) * | 1987-10-15 | 1997-03-25 | Chiron Corporation | Nucleic acid hybridization assay for hepatitis B virus DNA |
| WO1992002528A1 (en) * | 1990-07-27 | 1992-02-20 | Chiron Corporation | Incorporation of selectably clevable and/or abasic sites into oligonucleotide chains and reagents therefor |
| WO1992002526A1 (en) * | 1990-07-27 | 1992-02-20 | Chiron Corporation | Large comb-type branched polynucleotides |
| WO1995020320A1 (en) * | 1994-01-31 | 1995-08-03 | Trustees Of Boston University | Self-assembling multimeric nucleic acid constructs |
| WO1998004746A1 (en) * | 1996-07-31 | 1998-02-05 | Mount Sinai School Of Medicine Of The City University Of New York | Nucleic acid amplification method: ramification-extension amplification method (ram) |
Also Published As
| Publication number | Publication date |
|---|---|
| AU4824800A (en) | 2000-11-21 |
| AU782408B2 (en) | 2005-07-28 |
| WO2000068434A2 (en) | 2000-11-16 |
| JP2002543813A (en) | 2002-12-24 |
| CA2371843A1 (en) | 2000-11-16 |
| EP1196632A2 (en) | 2002-04-17 |
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