WO1997030179B1 - Determining exposure to ionizing radiation agent with persistent biological markers - Google Patents
Determining exposure to ionizing radiation agent with persistent biological markersInfo
- Publication number
- WO1997030179B1 WO1997030179B1 PCT/US1997/001972 US9701972W WO9730179B1 WO 1997030179 B1 WO1997030179 B1 WO 1997030179B1 US 9701972 W US9701972 W US 9701972W WO 9730179 B1 WO9730179 B1 WO 9730179B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- gene
- cells
- ionizing radiation
- population
- exposure
- Prior art date
Links
- 239000000090 biomarker Substances 0.000 title claims abstract 4
- 230000002085 persistent Effects 0.000 title abstract 2
- 239000003795 chemical substances by application Substances 0.000 title 1
- 210000004027 cells Anatomy 0.000 claims 11
- 210000002966 Serum Anatomy 0.000 claims 5
- 230000014509 gene expression Effects 0.000 claims 5
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 238000010240 RT-PCR analysis Methods 0.000 claims 1
- 238000004166 bioassay Methods 0.000 claims 1
- 210000002798 bone marrow cell Anatomy 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Abstract
Persistent biological indicators of exposure to ionizing radiation, particularly nucleic acid indicators, can be employed in determining whether a subject has been exposed to ionizing radiation. Such biological indicators can be indentified via the technique of differential display.
Claims
AMENDED CLAIMS
| received by the International Bureau on 24 September 1997 (24 09 97), original claims 1 and 25 amended, remaining claims unchanged (2 pages)]
1. A method of identifying a biological indicator of exposure to ionizing radiation, comprising the steps of: exposing a population of cells to ionizing radiation; using differential display to compare gene expression in the population of cells three weeks after exposure to the ionizing radiation to gene expression in a control population of cells not exposed to the ionizing radiation; and selecting a gene or gene fragment that has an altered level of gene expression m the exposed population of cells as compared to the control population of cells, which level of expression persists for at least three weeks following exposure to the ionizing radiation.
2. A method according to claim 1, additionally comprising a step of sequencing the gene or gene fragment
3. A method according to claim 1, wherein the step of exposing a population of cells to ionizing radiation is a step of exposing a culture of cells m vi tro .
4. A method according to claim 1, wherein the step of exposing a population of cells to ionizing radiation is a step of exposing cells m vivo .
5. A method according to claim 1, wherein the gene or gene fragment corresponds to a gene that encodes a serum amyloid.
6. A method according to claim 5, wherein the serum amyloid is murine serum amyloid A3.
7. A method according to claim 5, wherein the serum amyloid is human serum amyloid Al or A2.
25. A kit for detecting past exposure to ionizing radiation, comprising: primers specific for a gene or gene fragment which, upon exposure to ioninzing radiation, exhibits an altered level of expression that persists for at least three weeks following exposure to ionizing radiation; reagents for RT-PCR analysis; and instructions for using the primers and the reagents in an assay to determine whether cells removed from a subject contain the gene or gene fragment .
26. A kit as claimed in claim 25, wherein the cells are bone marrow cells.
27. A kit as claimed in claim 26, wherein the primers are specific to an SAA gene or fragment thereof .
STATEMENT UNDER ARTICLE 19(1)
In response to the International Search Report, mailed on July 24, 1997, the applicants have amended the claims by replacing present pages 25 and 28 with substitute pages 25 and 28 submitted herewith.
Claim 1 and claim 25 have been replaced with new claims 1 and 25. Claims 2-24 and claims 26-27 are as filed. Accordingly, claims 1-27 are presented for international preliminary examination.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9529401A JP2000504583A (en) | 1996-02-15 | 1997-02-18 | Determining exposure to ionizing radiation agents using persistent biological markers |
| EP97905832A EP0880605A2 (en) | 1996-02-15 | 1997-02-18 | Determining exposure to ionizing radiation agent with persistent biological markers |
| AU22629/97A AU2262997A (en) | 1996-02-15 | 1997-02-18 | Determining exposure to ionizing radiation agent with persistent biologic al markers |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/602,145 | 1996-02-15 | ||
| US08/602,145 US6025336A (en) | 1996-02-15 | 1996-02-15 | Determining exposure to ionizing radiation agent with persistent biological markers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1997030179A1 WO1997030179A1 (en) | 1997-08-21 |
| WO1997030179B1 true WO1997030179B1 (en) | 1997-11-06 |
Family
ID=24410152
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1997/001972 WO1997030179A1 (en) | 1996-02-15 | 1997-02-18 | Determining exposure to ionizing radiation agent with persistent biological markers |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6025336A (en) |
| EP (1) | EP0880605A2 (en) |
| JP (1) | JP2000504583A (en) |
| KR (1) | KR19990082584A (en) |
| CN (1) | CN1216069A (en) |
| AU (1) | AU2262997A (en) |
| CA (1) | CA2246387A1 (en) |
| WO (1) | WO1997030179A1 (en) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6172458B1 (en) | 1997-04-30 | 2001-01-09 | Tdk Corporation | Organic electroluminescent device with electrode of aluminum-lithium alloy |
| US7008768B1 (en) | 1999-02-26 | 2006-03-07 | The United States Of America As Represented By The Department Of Health And Human Services | Method for detecting radiation exposure |
| US20050014165A1 (en) * | 2003-07-18 | 2005-01-20 | California Pacific Medical Center | Biomarker panel for colorectal cancer |
| CA2552351A1 (en) | 2004-01-30 | 2005-09-09 | Dana-Farber Cancer Institute, Inc. | Method for determination and quantification of radiation or genotoxin exposure |
| KR100735731B1 (en) | 2005-07-22 | 2007-07-06 | 이시혁 | Analysis system on molecular biomarkers of Pardosa astrigera for the effect of heavy metal exposure |
| US20080076122A1 (en) * | 2006-09-26 | 2008-03-27 | The Regents Of The University Of California | Characterizing exposure to ionizing radiation |
| US20080227130A1 (en) * | 2007-03-15 | 2008-09-18 | Tulsee Satish Doshi | Method for detecting exposure to ionizing radiation |
| EP2167683A4 (en) | 2007-05-31 | 2010-10-20 | California Pacific Med Center | Method to predict or diagnose a gastrointestinal disorder or disease |
| WO2009015299A1 (en) | 2007-07-26 | 2009-01-29 | California Pacific Medical Center | Method to predict or diagnose a gastrointestinal disorder or disease |
| US20140315742A1 (en) | 2011-09-29 | 2014-10-23 | Meso Scale Technologies, Llc | Biodosimetry panels and methods |
| CN105803049A (en) * | 2014-12-29 | 2016-07-27 | 中国辐射防护研究院 | Method for screening biomarkers of ionizing radiation as well as application of SZT2 protein determined by method |
| CN107090496A (en) * | 2017-03-28 | 2017-08-25 | 南方医科大学 | A kind of detection method of 8Gy ionising radiations |
| CN107015261A (en) * | 2017-04-13 | 2017-08-04 | 南方医科大学 | A kind of detection method of 6.5Gy ionising radiations |
| CN111621559A (en) * | 2020-06-11 | 2020-09-04 | 北京市化工职业病防治院(北京市职业病防治研究院) | Screening of Low dose ionizing radiation Effector genes in Association of transcriptome and proteome data |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5346814A (en) * | 1992-10-14 | 1994-09-13 | Board Of Trustees Of The Leland Stanford Jr. University | Method of detecting cell response to cell-damaging energy |
-
1996
- 1996-02-15 US US08/602,145 patent/US6025336A/en not_active Expired - Fee Related
-
1997
- 1997-02-18 WO PCT/US1997/001972 patent/WO1997030179A1/en not_active Application Discontinuation
- 1997-02-18 CA CA002246387A patent/CA2246387A1/en not_active Abandoned
- 1997-02-18 AU AU22629/97A patent/AU2262997A/en not_active Abandoned
- 1997-02-18 JP JP9529401A patent/JP2000504583A/en active Pending
- 1997-02-18 KR KR1019980706322A patent/KR19990082584A/en not_active Application Discontinuation
- 1997-02-18 CN CN97193746A patent/CN1216069A/en active Pending
- 1997-02-18 EP EP97905832A patent/EP0880605A2/en not_active Withdrawn
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