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US1331207A - Anaerobic chamber - Google Patents

Anaerobic chamber Download PDF

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Publication number
US1331207A
US1331207A US260037A US26003718A US1331207A US 1331207 A US1331207 A US 1331207A US 260037 A US260037 A US 260037A US 26003718 A US26003718 A US 26003718A US 1331207 A US1331207 A US 1331207A
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US
United States
Prior art keywords
box
chamber
gas
air
lamp
Prior art date
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Expired - Lifetime
Application number
US260037A
Inventor
Mouneyrat Antoine
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Individual
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Individual
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Filing date
Publication date
Priority to FR488636A priority Critical patent/FR488636A/en
Priority to GB259418A priority patent/GB122515A/en
Application filed by Individual filed Critical Individual
Priority to US260037A priority patent/US1331207A/en
Application granted granted Critical
Publication of US1331207A publication Critical patent/US1331207A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B25HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
    • B25JMANIPULATORS; CHAMBERS PROVIDED WITH MANIPULATION DEVICES
    • B25J21/00Chambers provided with manipulation devices
    • B25J21/02Glove-boxes, i.e. chambers in which manipulations are performed by the human hands in gloves built into the chamber walls; Gloves therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/80Elimination or reduction of contamination by undersired ferments, e.g. aseptic cultivation
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/801Anerobic cultivation

Definitions

  • the present invention relates to anarob1c chambers.
  • the object of the invention is to provide a special chamber for allowing any substance to be safely manipulated in any gas and out of contact with the external atmosphere.
  • Figure 1 is a perspective view.
  • Fig. 2 is a detail view showing the method of mounting the manipulating gloves in the openings of the chamber.
  • Fig. 3 shows on a larger scale an arrangement for detecting the presence of air in the chamber.
  • the anaerobic chamber of the present invention consists of an entirely closed box a preferably of parallelepiped shape. On the front side of said box are provided three openings b c el and on the rear side two openings b and d' facing the openings b and d respectively in the front side.
  • the openings b and b and d and d are large enough to allow free passage to the hands and arms of the operator; the central opening c is large enough for the admission of the various obJects and apparatus to be used in the box.
  • the said object is introduced at the end of the rubber tube.
  • the end of said tube being then held firmly, the object is caused to move forward in the tube by working on it from the outside; when the object has reached the opening c the operator takes hold of it with his hands after having inserted the latter in the gloves e.
  • a quick and easy method consists in introducing the gas by means of a radiator g placed at a suitable position in the apparatus, for instance as shown in Fig. l on the right hand side of the apparatus.
  • This radiator consists of a tube/lt extending into the interior of the box and connected with a series of small vertical tubes perforated at intervals with holes through which the gas for l lbox through the holes jin the left hand side of the box Fig. 1.
  • the holes y' are closed by means of Stoppers or taps.
  • the gas inlet is closed by means of a tap or other means.
  • a gas analysis could be made by collecting gas from the interior of the box, for instance through one of the openings j; however it is much simpler and more expedient to operate in the following manner
  • a benzene or the like lamp lo is placed, for instance, at the right hand side near the front of the box. Said sov lamp can be lighted at will by means of an electric current, but will not burn unless the latmosphere of the box contains oxygen.
  • Anaccumulator Z and a sparking coil m are placed outside the box and a lamp lo provided with a copper igniter o is placed inside.
  • the positive terminal of the aclighting the lamp is as folcumulator is connected to the terminal p If oxygen is' present in the box the lamp is lighted by the sparks produced between the igniter o and the burner of the lamp; if there is no oxygen present the lamp does not light.
  • the switch g enables the lighting sparks to be passed or stopped at will. If the lamp lights it indicates that oxygen is present in the box, in which case the air must again be displaced with nitrogen.
  • the chamber of the present invention may be used for many purposes, but it will suffice to mention some of them 1. With such a chamber one can safely manipulate substances which evolve the most dangerous vapors.
  • Any substance can be manipulated in any gas without fear of such substance undergoing alteration through contact with the outer atmosphere.
  • An anaerobic chamber for safely manipulating lany substance in any gasand ⁇ out of contact with air comprising a completely closed box provided with a plurality of manipulating hand holes, long soft manipulating gloves extending through said hand holes into the interior of said box, each glove being firmly secured with its open end to one of the said hand holes so as to make an air tight joint, an opening provided with a long rubber adapted to Vprevent all communication between the interior of the box and the outer air and serving to introduce the objects to be manipulated into said box, and means for removing air from said box.
  • An anarobic box as set forth in claim 1 in which the air is displaced from the box by a current of gas discharged from a jet provided within said box.
  • means for detecting the presence of air in the box comprising a benzene or the like lamp at the top of which electric sparks are produced, which sparks light said lamp when any oxygen is present inside the box.

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  • Engineering & Computer Science (AREA)
  • Robotics (AREA)
  • Mechanical Engineering (AREA)
  • Sampling And Sample Adjustment (AREA)

Description

A. MOUN'EYRAT. ANA'ROBIG CHAMBER.
APPLICATION FILED OCT. 28, 9l8.
AUUPqNEY A. MOUNEYRAT. ANA'E'ROBIC CHAMBER.
APPLICATION FILED 0CT.28, |918. 1,331,207. Patented Feb. 17,1920.
2 SHEETS-SHEET 2.
i ANTOINE MOUNEYRAT, OF PARIS, FRANCE.
ANARoBIc CHAMBER.
Specification of Letters Patent.. Patented Feb. 17, 1920.
Application led October 28, 1918. Serial No. 260,037.
Z all lwhom t may concern.'
Be it known that I, ANTOINE MOUNEYRAT, a citizen of the Republic of France, and resident of Paris, France, (post-oflice address Rue de lAbb de lEpe,) have invented a new and usefulAnarobic Chamber, which improvements are fully set forth in the following specification.
The present invention relates to anarob1c chambers.
The object of the invention is to provide a special chamber for allowing any substance to be safely manipulated in any gas and out of contact with the external atmosphere.
In order to understand the invention more clearly reference is made to the accompanying drawings which show by way of example one way of carrying out the invention.
Figure 1 is a perspective view.
Fig. 2 is a detail view showing the method of mounting the manipulating gloves in the openings of the chamber.
Fig. 3 shows on a larger scale an arrangement for detecting the presence of air in the chamber.
The anaerobic chamber of the present invention consists of an entirely closed box a preferably of parallelepiped shape. On the front side of said box are provided three openings b c el and on the rear side two openings b and d' facing the openings b and d respectively in the front side. The openings b and b and d and d are large enough to allow free passage to the hands and arms of the operator; the central opening c is large enough for the admission of the various obJects and apparatus to be used in the box.
Long flexible rubber gloves e are inserted into the interiorof the box through each of said openings b b and d d as shown in Fig. 2. The wrists of said gloves are turned over and the overlapping parts are fastened at f to flanges provided around the openings b b d d. The overlapping parts of the glove e are firmly bound around the flanges. By means of these gloves the operator can work in the interior of the box withoutfear of the external air gaining access to the interior. i
The only part Where air could possibly enter the box is through the opening 0. In order to prevent such access of air a large exible rubber tube, of about 2 to 311: feet in length is secured over the opening c and hangs down outside the box. This rubber tube plays in the first place the same part as the rubber tube which hangs down from a balloon that is charged with hydrogen or coal gas; but it serves also as will be explained, as an air-lock which allows any required objects or chemical products to be inserted in and removed from the box without fear of mixing the outer atmosphere with the interior atmosphere of the box.
To insert any object into the box the said object is introduced at the end of the rubber tube. The end of said tube being then held firmly, the object is caused to move forward in the tube by working on it from the outside; when the object has reached the opening c the operator takes hold of it with his hands after having inserted the latter in the gloves e.
In order to produce the necessary atmosphere in the interior of the box the desired gas can be introduced by various means. A quick and easy method consists in introducing the gas by means of a radiator g placed at a suitable position in the apparatus, for instance as shown in Fig. l on the right hand side of the apparatus. This radiator consists of a tube/lt extending into the interior of the box and connected with a series of small vertical tubes perforated at intervals with holes through which the gas for l lbox through the holes jin the left hand side of the box Fig. 1. As soon as all the air has been driven out, the holes y' are closed by means of Stoppers or taps. The gas inlet is closed by means of a tap or other means.
If the box is charged with an inert gas such as nitrogen, it is necessary to be able to ascertain-at any moment whether the gas has become mixed with oxygen from' the outer atmosphere.
In order to ascertain this a gas analysis could be made by collecting gas from the interior of the box, for instance through one of the openings j; however it is much simpler and more expedient to operate in the following manner As shown in Fig. 3 a benzene or the like lamp lo is placed, for instance, at the right hand side near the front of the box. Said sov lamp can be lighted at will by means of an electric current, but will not burn unless the latmosphere of the box contains oxygen.
The method of lows Anaccumulator Z and a sparking coil m are placed outside the box and a lamp lo provided with a copper igniter o is placed inside. The positive terminal of the aclighting the lamp is as folcumulator is connected to the terminal p If oxygen is' present in the box the lamp is lighted by the sparks produced between the igniter o and the burner of the lamp; if there is no oxygen present the lamp does not light. The switch g enables the lighting sparks to be passed or stopped at will. If the lamp lights it indicates that oxygen is present in the box, in which case the air must again be displaced with nitrogen.
The chamber of the present invention may be used for many purposes, but it will suffice to mention some of them 1. With such a chamber one can safely manipulate substances which evolve the most dangerous vapors.
2. Any substance can be manipulated in any gas without fear of such substance undergoing alteration through contact with the outer atmosphere.
3; Inasmuch as the interior of the chamber can be heated electrically, chemical re` actions can be carried out in any gas `at any desired temperatures.
`4. By` the aid of this 'chamber the behavior of vegetable cultures and microbes in a gas of any composition can be studied.
Having now particularly described and ascertained the nature of my said invention and in fwhat manner the same is to be performed, I declare that what I claim is 1. An anaerobic chamber for safely manipulating lany substance in any gasand` out of contact with air, comprising a completely closed box provided with a plurality of manipulating hand holes, long soft manipulating gloves extending through said hand holes into the interior of said box, each glove being firmly secured with its open end to one of the said hand holes so as to make an air tight joint, an opening provided with a long rubber adapted to Vprevent all communication between the interior of the box and the outer air and serving to introduce the objects to be manipulated into said box, and means for removing air from said box.
2. An anarobic box as set forth in claim 1 in which the air is displaced from the box by a current of gas discharged from a jet provided within said box.
3. In an apparatus as set forth in claim 2, means for detecting the presence of air in the box comprising a benzene or the like lamp at the top of which electric sparks are produced, which sparks light said lamp when any oxygen is present inside the box.
In testimony whereof I have signed thisv specification in the presence of two subscribing witnesses.
DR'. A. MOUNEYRAT.
Witnesses:
BRUGio'rfrI, JOHN F. SIMONS.
US260037A 1918-02-13 1918-10-28 Anaerobic chamber Expired - Lifetime US1331207A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR488636A FR488636A (en) 1918-10-28 1918-01-28 Anaerobic case
GB259418A GB122515A (en) 1918-02-13 1918-02-13 Improvements in or relating to Chambers for Effecting Manipulations Out of Contact with the Air or in Any Desired Atmosphere.
US260037A US1331207A (en) 1918-10-28 1918-10-28 Anaerobic chamber

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US260037A US1331207A (en) 1918-10-28 1918-10-28 Anaerobic chamber

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US1331207A true US1331207A (en) 1920-02-17

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US260037A Expired - Lifetime US1331207A (en) 1918-02-13 1918-10-28 Anaerobic chamber

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2425725A (en) * 1941-08-26 1947-08-19 Bell Telephone Labor Inc Method of casting and rapid analysis of alloys
US2741410A (en) * 1951-12-06 1956-04-10 Gen Electric Method and apparatus for handling contaminated gloves
US2786740A (en) * 1954-07-09 1957-03-26 Air Reduction Special atmosphere device
US4086060A (en) * 1976-10-22 1978-04-25 Jocelyn Dickson Disposable manipulative laboratory device for transferring biological fluids
US20160251206A1 (en) * 2012-05-03 2016-09-01 Vanrx Pharmaceuticals, Ins. Cover removal system for use in controlled environment enclosures
US10343293B1 (en) * 2017-12-20 2019-07-09 Troy Taylor Portable enclosure for hazardous processing
US20210371148A1 (en) * 2012-05-03 2021-12-02 Vanrx Pharmasystems Inc. Cover removal system for use in controlled environment enclosures
US11981473B2 (en) 2020-09-27 2024-05-14 V Anrx Pharmasystems Inc. Cover removal system for use in controlled environment enclosures
US12065347B2 (en) 2012-05-03 2024-08-20 Vanrx Pharmasystems Inc. Cover removal system for use in controlled environment enclosures

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2425725A (en) * 1941-08-26 1947-08-19 Bell Telephone Labor Inc Method of casting and rapid analysis of alloys
US2741410A (en) * 1951-12-06 1956-04-10 Gen Electric Method and apparatus for handling contaminated gloves
US2786740A (en) * 1954-07-09 1957-03-26 Air Reduction Special atmosphere device
US4086060A (en) * 1976-10-22 1978-04-25 Jocelyn Dickson Disposable manipulative laboratory device for transferring biological fluids
US4129419A (en) * 1976-10-22 1978-12-12 Jocelyn Dickson Disposable laboratory device for transfer of fluids to a centrifugal analyzer head
US20160251206A1 (en) * 2012-05-03 2016-09-01 Vanrx Pharmaceuticals, Ins. Cover removal system for use in controlled environment enclosures
US10081527B2 (en) * 2012-05-03 2018-09-25 Vanrx Pharmasystems Inc. Cover removal system for use in controlled environment enclosures
US20210371148A1 (en) * 2012-05-03 2021-12-02 Vanrx Pharmasystems Inc. Cover removal system for use in controlled environment enclosures
US12065347B2 (en) 2012-05-03 2024-08-20 Vanrx Pharmasystems Inc. Cover removal system for use in controlled environment enclosures
US10343293B1 (en) * 2017-12-20 2019-07-09 Troy Taylor Portable enclosure for hazardous processing
US11981473B2 (en) 2020-09-27 2024-05-14 V Anrx Pharmasystems Inc. Cover removal system for use in controlled environment enclosures

Also Published As

Publication number Publication date
FR488636A (en) 1918-10-23

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