SU1534490A1 - Method of modeling acute hematogenic osteomyelitis - Google Patents

Abstract

The invention relates to experimental medicine and can be used to model acute hematogenous osteomyelitis. The goal is to increase the reproducibility of the method. The method is carried out by applying a pulse to ultrasound with a resonance frequency of 2.34-1.92 MHz, a focal length of 70 mm, an opening angle of 36-72 °, a pulse duration of 0.1 to 1000 ms, an output power of 250 W for 4–5 s. Then, a suspension of a daily culture of Staphylococcus aureus is administered intraarterially at the base of the limb at the rate of 20 thousand bacterial bodies per 1 kg of animal weight. Next, venous "cryozhgut" is imposed for 1 h with local cooling of the skin from 23 to 25 ° C with subsequent decompression within 30 min at a pressure of 0.5 atm.

Description

The invention relates to experimental medicine and can be used to model acute hematogenous osteomyelitis.

The purpose of the invention is to increase the reproducibility of the method.

The method is carried out by pulsing ultrasound with an animal with a resonance frequency of 2.34-1.92 MHz, a focal length of 70 mm, an opening angle of 36 --- 720, pulse duration from 0.1 to 1000 ms, intervals between pulses 0 , 1 s, rhythm of 50 pulses / s, power ng amplifier output 250 W for 4--5 s. Then intra-arterially at the base of the limb is introduced a suspension of daily culture of Staphylococcus aureus at the rate of 20 thousand bacterial bodies per 1 kg of animal weight. Then, venous “is implanted for 1 h with local cooling of the skin from 23 to 25 ° C with subsequent decompression for 30 min at a pressure of 0.5 atm.

Example. Rabbit weighing 2800 g is fixed to the machine, shave the hair with the rear curve of the limb. A focusing emitter with a resonant frequency of 2.34 MHz, an ultrasonic oscillator ESA-1 tuned to this frequency, and a power amplifier are used as the source of ultrasound. The emitter was stirred into a casing filled with distilled water (the temperature of the water is maintained at 38 ° C). The hind limb of the rabbit was positioned and fastened above and by the radiator so that the focus of the focus (1 70 mm) was the bone-marrow channel of the femur in the lower third, which was located on average 10-15 mm from the skin surface. For 4 s, the bone was voiced with a pulse duration of 0.1 ms, intervals between pulses of 0.1 s, and a rhythm of 50 pulses / s. The opening angle of the focusing device is 72 °, the output power of the amplifier is 250 W. The limb was removed from the casing, drained with napkins, lubricated with alcohol, fixed

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with

Whether a rabbit is to a machine. Under local infiltration anesthesia with a 0.5% novocaine solution with a linear incision of 15–20 mm, the femoral artery was exposed in the upper third of the thigh, punctured with a thin needle and 56 thousand microorganisms of daylight staphylococcus (strain 209) were introduced in 2 ml a sterile isotonic solution of sodium chloride was removed, a venous solution was applied to the base of the extremity, the wound stitches were applied, the cuff pressure was 0.5 atm, the temperature of the underlying skin was maintained within 25 ° C the compression was stopped and the same was used to compress the tissues with a cuff pressure of 0.5 atm during 30 minutes, then the crooked / Kivi animal was removed and dismounted from the i Mepei 3 rabbit in l, sparing the infarted limb during movement , on the 7th on the front inner rim pxnoc.ru, the right hip in the lower third appeared mrip hlosg gkansy, which to in m increased in size 1 k pe s 10 after infection with xylope using the isotope Si 85 The meaning of nnpe isotope accumulation in the field of diacia.ibHioio metaenif d misfortunes IIULHIR and its information

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five

The animal was removed from the experiment on the 10th day after infection.

The foci of cellular disintegration of tissues in the bone marrow spaces and the channel of the lower third of the femur, characteristic of the course of acute osteomyelitis, were histologically determined.

Claims (1)

Invention Formula A method of modeling acute hematogenous osteomyelitis by introducing a microbial culture into the bloodstream and exposing a bone to a physical factor, characterized in that, in order to increase the reproducibility of the method, the bone is pulsed with ultrasound with a resonant frequency of 2.34-1.92 MHz, the focal length 70 mm, an angle of 36–72 °, a pulse duration of 0.1 to 1000 ms, an output power of 250 W for 4–5 s, then a suspension of daily culture of Staphylococcus aureus is intraarterially administered at the base of the limb Single rate of 20 thousands bacteria per 1 kg body weight of the animal, more superimposed venous "kriozhgut for 1 hour with local skin ohpazhdeniem from 23 to 25 ° C with subsequent depressurization for 30 minutes at a pressure of 0.5 atm