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RU2016147208A - Method for isolating biologically active proanthocyanidins - Google Patents

Method for isolating biologically active proanthocyanidins Download PDF

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Publication number
RU2016147208A
RU2016147208A RU2016147208A RU2016147208A RU2016147208A RU 2016147208 A RU2016147208 A RU 2016147208A RU 2016147208 A RU2016147208 A RU 2016147208A RU 2016147208 A RU2016147208 A RU 2016147208A RU 2016147208 A RU2016147208 A RU 2016147208A
Authority
RU
Russia
Prior art keywords
biologically active
proanthocyanidins
evaporated
isolating biologically
extracted
Prior art date
Application number
RU2016147208A
Other languages
Russian (ru)
Other versions
RU2664709C2 (en
RU2016147208A3 (en
Inventor
Михаил Юрьевич Ахлебинин
Владимир Анатольевич Огурцов
Александр Андреевич Абрамов
Original Assignee
Михаил Юрьевич Ахлебинин
Владимир Анатольевич Огурцов
Александр Андреевич Абрамов
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Михаил Юрьевич Ахлебинин, Владимир Анатольевич Огурцов, Александр Андреевич Абрамов filed Critical Михаил Юрьевич Ахлебинин
Priority to RU2016147208A priority Critical patent/RU2664709C2/en
Publication of RU2016147208A3 publication Critical patent/RU2016147208A3/ru
Publication of RU2016147208A publication Critical patent/RU2016147208A/en
Application granted granted Critical
Publication of RU2664709C2 publication Critical patent/RU2664709C2/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/45—Ericaceae or Vacciniaceae (Heath or Blueberry family), e.g. blueberry, cranberry or bilberry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Pyrane Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Claims (1)

Способ выделения биологически активных проантоцианидинов, характеризующийся тем, что измельченные листья голубики (Vaccinium uliginosum) экстрагируют метанолом в течение 3-х часов при температуре 30-35°С, упаривают спиртовой раствор до 1/10 первоначального объема, разбавляют 4-кратным количеством воды и экстрагируют последовательно хлороформом, эфиром и этилацетатом, после чего полученный водный раствор упаривают и хроматографируют на колонке с силикагелем с размером частиц 0,040-0,063 мм, используя в качестве элюента смесь хлористого метилена с метанолом, взятых в соотношении 1:1, и выделяют биологически активную фракцию с Rf 0,29.A method for isolating biologically active proanthocyanidins, characterized in that the crushed blueberry leaves (Vaccinium uliginosum) are extracted with methanol for 3 hours at a temperature of 30-35 ° C, the alcohol solution is evaporated to 1/10 of the original volume, diluted with 4 times the amount of water and extracted successively with chloroform, ether and ethyl acetate, after which the resulting aqueous solution was evaporated and chromatographed on a silica gel column with a particle size of 0.040-0.063 mm, using a mixture of methylene chloride and methane as eluent ohms in the ratio 1: 1 and isolated biologically active fraction with R f 0,29.
RU2016147208A 2016-12-01 2016-12-01 Method for isolation of biologically active proanthocyanidins RU2664709C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2016147208A RU2664709C2 (en) 2016-12-01 2016-12-01 Method for isolation of biologically active proanthocyanidins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2016147208A RU2664709C2 (en) 2016-12-01 2016-12-01 Method for isolation of biologically active proanthocyanidins

Publications (3)

Publication Number Publication Date
RU2016147208A3 RU2016147208A3 (en) 2018-06-06
RU2016147208A true RU2016147208A (en) 2018-06-06
RU2664709C2 RU2664709C2 (en) 2018-08-21

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU2016147208A RU2664709C2 (en) 2016-12-01 2016-12-01 Method for isolation of biologically active proanthocyanidins

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Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1014969E (en) * 1997-09-09 2009-02-17 Univ Rutgers Plant proanthocyanidin extract effective at inhibiting adherence of bacteria with p-type fimbriae to surfaces
RU2220172C1 (en) * 2002-07-17 2003-12-27 Государственное образовательное учреждение Воронежская государственная технологическая академия Method for production of anthocyan dye from flower raw material
ITMI20051485A1 (en) * 2005-07-29 2007-01-30 Indena Spa GRAPE SEED EXTRACT AVAILABLE FOR FRACTION ON RESIN

Also Published As

Publication number Publication date
RU2664709C2 (en) 2018-08-21
RU2016147208A3 (en) 2018-06-06

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20191202