KR20240084704A - Feed additive composition for reducing methane emission - Google Patents

Abstract

According to an embodiment of the present invention, a feed additive composition for methane reduction is provided. The feed additive composition includes unsaturated fatty acids, and the unsaturated fatty acids include soybean oil, avocado oil, linoleic acid, linolenic acid, and oleic acid. It may include one or more types selected from.

Description

Feed additive composition for reducing methane {FEED ADDITIVE COMPOSITION FOR REDUCING METHANE EMISSION}

The present invention relates to a feed additive composition for methane reduction.

As international interest in the problem of global warming caused by greenhouse gases such as carbon dioxide (CO ₂ ), methane gas (CH ₄ ), and nitrous oxide (N ₂ O) increases worldwide, reduction of greenhouse gases is required to respond to climate change. There is a trend toward making it mandatory.

For example, among greenhouse gases, methane gas is generated during the digestive process through intestinal fermentation in ruminants. Feed consumed by ruminants is decomposed into carbon dioxide, hydrogen, ammonia, etc. by microorganisms, and carbon dioxide and hydrogen are produced into methane by methanogenic bacteria. This methane cannot be absorbed in the body of ruminants and is released from the rumen through burping. It is common for it to be released.

When methane is produced within the rumen of ruminants, environmental problems arise due to global warming and feed use efficiency decreases. To solve this problem, various methods are being studied to reduce methane gas emitted from ruminants.

For example, methods for reducing methane gas include adding antibiotics such as monensin to feed, removing protozoa in the rumen, adding halogenated compounds to feed, and probiotics. ), etc.

However, adding antibiotics to livestock feed can ultimately be harmful to the human body, and removing protozoa can reduce fiber. In addition, adding halogenated compounds to feed may not maintain the methane gas suppression effect and may cause safety issues such as persistence in livestock products, and feeding probiotics may affect the fermentation pattern of the probiotics or the strain added during the methane gas production experiment. There are problems that can appear differently.

Accordingly, technology that can efficiently reduce methane gas emitted from livestock without negatively affecting livestock productivity is required.

The present invention is intended to solve the above problems, and its purpose is to provide a feed additive composition for methane reduction.

The technical problems of the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly understood by those skilled in the art from the description below.

According to an embodiment of the present invention, a feed additive composition for methane reduction is provided. The feed additive composition includes unsaturated fatty acids, and the unsaturated fatty acids include soybean oil, avocado oil, linoleic acid, linolenic acid, and oleic acid. It may include one or more types selected from.

According to the present invention, environmental pollution can be prevented by efficiently reducing methane gas emitted from ruminant animals.

Additionally, according to the present invention, animal odor caused by ammonia, hydrogen sulfide, etc. can be minimized.

In addition, according to the present invention, the productivity of ruminants can be increased by reducing energy loss due to the emission of methane gas while improving the efficiency of feed use by ruminants.

Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by a person skilled in the art to which the present invention pertains. In general, the nomenclature used herein is well known and commonly used in the art. Additionally, when describing embodiments of the present invention, if detailed descriptions of related known configurations or functions are judged to impede understanding of the embodiments of the present invention, the detailed descriptions will be omitted. In addition, embodiments of the present invention will be described below, but the technical idea of the present invention is not limited or limited thereto and may be modified and implemented in various ways by those skilled in the art.

In this specification, when a part includes a certain element, this does not mean excluding other elements, but may further include other elements, unless specifically stated to the contrary. In this specification, the term and/or includes a combination of a plurality of related items or any one item among a plurality of related items.

According to an embodiment of the present invention, a feed additive composition is provided. Feed additives may be added to the feed when feeding livestock to promote metabolism in livestock, improve feed efficiency, and supplement deficiencies.

The feed additive composition of the present invention contains unsaturated fatty acids. Here, unsaturated fatty acid refers to a chain-shaped compound having an unsaturated carbon-carbon bond and a carboxy group, and may refer to a component consisting of an even number of carbon atoms of about 12 to 20.

Unsaturated fatty acids can reduce the production of methane by preferentially combining with hydrogen ions before methane is produced by combining carbon sources and hydrogen ions by methanogenic bacteria (i.e. methanogens) in animals. Specifically, this is because unsaturated carbon-carbon bonds exist in unsaturated fatty acids, and due to the unsaturated bonds, unsaturated fatty acids can preferentially combine with hydrogen ions.

In an embodiment, the unsaturated fatty acid may include one or more selected from the group consisting of soybean oil, avocado oil, linoleic acid, linolenic acid, and oleic acid. However, it is not limited to this.

In examples, unsaturated fatty acids may be included in an amount of 0.1 to 20% by weight based on the total weight of the composition, but are not limited thereto. When the unsaturated fatty acid is included in the content within the above range, the effect intended by the present invention is obtained, and it may be appropriate to use the content within the above range in terms of cost-effectiveness.

In an embodiment, the feed additive composition of the present invention may further include natural extracts and/or mineral concentrates. Since natural extracts and/or mineral concentrates contain substances rich in biological activity such as various vitamins, enzymes, minerals, and fiber, they can provide effects such as suppressing the activity of methane-producing bacteria and suppressing microorganisms that cause bad odors. It can lead to improved appetite and digestion in livestock.

In an embodiment, natural extract means extracting plants including seaweed, trees, mushrooms, grass, etc. using a solvent. The plant used to produce the extract may be a raw material that has not gone through a grinding or grinding process, or a cut product that has gone through a grinding or grinding process, and may include all parts of the plant.

In an example, the solvent used during plant extraction is water (or distilled water); lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol, and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol, propylene glycol, methylpropanediol, and 1,2-hexanediol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof can be used. However, it is not limited to this, and various solvents applicable in the relevant technical field can be used alone or in combination.

In an embodiment, the extraction method of the plant may be an extraction method commonly used in the art, such as hot water extraction, immersion extraction, reflux extraction, supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, or ultrasonic extraction. . At this time, natural extracts may include all extracts, fractions, dilutions, and concentrates obtained in each step such as extraction, fractionation, or purification.

In some embodiments, the natural extract may be in a liquid form, a solid form obtained by drying a liquid extract, or a powder form obtained by pulverizing a solid extract. At this time, various drying methods commonly used in the technical field, such as spray drying, freeze drying, vacuum drying, and hot air drying, may be applied.

In an example, the natural extract is from the group consisting of Ecklonia cava extract, kelp extract, seaweed extract, bear skin extract, rhubarb extract, garlic extract, juniper extract, rhododendron extract, stevia extract, apple extract, pear extract, green tea extract, and persimmon extract. It may include one or more selected types, but is not limited thereto.

In an embodiment, the mineral concentrate contains potassium (K), calcium (Ca), sodium (Na), magnesium (Mg), phosphorus (P), iron (Fe), silicon (Si), manganese (Mn), zinc ( It may include one or more selected from the group consisting of Zn), chromium (Cr), copper (Cu), selenium (Se), molybdenum (Mo), bromine (Br), iodine (I), and barium (Ba). However, it is not limited to this.

In an embodiment, the natural extract and mineral concentrate may each be included in an amount of 5 to 10% by weight based on the total weight of the composition, but are not limited thereto. When the natural extract and/or mineral concentrate is included in the content within the above range, the effect intended by the present invention is obtained, and it may be appropriate to use the above range in terms of cost-effectiveness.

In an embodiment, the feed additive composition of the present invention may be fed to livestock alone, or may be added to and mixed with a feed composition and fed to livestock. For example, the feed composition may mean any natural or artificial diet, meal, etc., or a component of the meal, for or suitable for eating, ingestion, and digestion by livestock.

In an embodiment, the livestock fed with the feed additive composition may be ruminants of the camelid, deer family, deer family, giraffe family, and bovine family among the mammalian order. Here, ruminants refer to animals that have a rumen and chew the cud to digest food, and may include, for example, cows, goats, sheep, giraffes, deer, buffaloes, or camels, but are not limited thereto.

In an embodiment, the feed composition may include essential nutrients that must be supplied from the feed to maintain the safety and growth of the animal in addition to the feed additive composition. For example, the feed composition may include one or more selected from the group consisting of energy, amino acids, minerals, vitamins, and water, but is not limited thereto.

In addition, for example, the feed composition can be manufactured by methods known in the art, and includes, but is not limited to, general rice straw, wild grass, pasture, ansylage, hay, wild grass, etc., and is used for raising animals. Any feed that can be used is fine.

In embodiments, the feed additive composition and/or feed composition may be manufactured in formulations such as powder and pellets, but is not limited thereto and may be manufactured in various formulations known in the art.

In an embodiment, the feed additive composition may be mixed in an amount of about 10 to 500 g based on 1 kg of feed composition, but is not limited thereto. In addition, after the feed additive composition is completely mixed, it may be supplied as a mash, or may be subjected to pelletization, expansion and/or extrusion processes through additional processing, but is not limited thereto.

As such, by containing unsaturated fatty acids, the feed additive composition according to the present invention can reduce the amount of methane emitted from animals that consume it, thereby improving the efficiency of feed use by ruminants and reducing the amount of methane gas released by methane gas. By reducing energy loss, the productivity of ruminant animals can be increased.

In addition, the feed additive composition according to the present invention can minimize animal odor caused by ammonia, hydrogen sulfide, etc. by further containing natural extracts and/or mineral concentrates in addition to unsaturated fatty acids.

[Experimental example]

Below, examples are shown to further explain the present invention in more detail, but the present invention is not limited thereto.

Preparation of examples and comparative examples

(1) Preparation of examples and comparative examples

Examples of compositions according to the present invention were prepared using the ingredients listed in Table 1 below. The unit of ingredient content in Table 1 is weight%.

ingredient Example Comparative example unsaturated fatty acids oleic acid 20 - saturated fatty acids palmitic acid - 20 Natural extracts 10 10 mineral concentrate 10 10 Purified water To 100 To 100 total 100 100

Specifically, unsaturated or saturated fatty acids were added to purified water and mixed homogeneously with stirring at a temperature of 65°C, and then natural extracts and mineral concentrates were sequentially added and stirred. Afterwards, it was mixed homogeneously and cooled to room temperature to prepare a feed additive composition.

Experiment example

(1) Experimental Example 1: Changes in total gas and methane production amount

An in vitro test was conducted to determine changes in total gas and methane production for the above Examples and Comparative Examples, and the results are shown in Table 2.

The specific experimental method is as follows. For each of the Examples and Comparative Examples, 0.2 g of substrate (TDN 70%, CP 9.6%) was placed in a serum bottle, the feed additive composition was added, and the mixture was dried until methanol completely volatilized. Add 20 ml of rumen fluid diluted with McDougall buffer mixture (pH 6.8) to the serum bottle containing the substrate and feed additive composition and incubate at 37°C to determine pH, dry matter digestibility, total gas production, methane production, and volatile fatty acid production after 24 hours. , Ammonia production was measured and its effect on fermentation characteristics in the rumen was analyzed.

division Example Comparative example pH 6.76 6.78 Dry matter digestibility (%) 41.53 36.87 Total gas production (mL) 86.66 115.47 Methane production (mL) 13.66 20.13 Carbon dioxide produced (mL) 69.62 74.42 Volatile fatty acid production (mmol) 56.07 63.53 Ammonia production (mg·dL ^-1 ) 10.13 11.67

As a result of the experiment, the Examples and Comparative Examples did not show significant differences in pH and dry matter digestibility, but it was confirmed that the total gas production amount, methane production amount, and carbon dioxide production amount in the Example were measured at significantly lower values than the Comparative Example.

From this, it was found that the feed additive composition of the present invention is effective in reducing methane production in the rumen.

(2) Experimental Example 2: Confirmation of animal odor reduction efficacy

In order to confirm the effectiveness of reducing odor in the Examples and Comparative Examples, the odor of excrement was evaluated 48 hours after feeding the feed composition containing each feed additive composition to cattle, and the results are shown in Table 3. It was. At this time, the case where no feed additive was added was set as the control group and compared.

division Whether the excrement smells Example Almost no odor Comparative example stink control group a strong odor

As a result of the experiment, it was found that when the feed additive composition of the example was fed, there was almost no smell of excrement compared to the comparative example and control group.

Meanwhile, in the case of the comparative example in which unsaturated fatty acids were replaced with saturated fatty acids in the feed additive composition of the examples and the control group in which no sample additive composition was mixed, it was confirmed that an unpleasant odor was generated from the excrement.

As the specific parts of the present invention have been described in detail above, those skilled in the art will understand that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (1)

A feed additive composition for methane reduction,
Contains unsaturated fatty acids,
The unsaturated fatty acid is a feed additive containing at least one selected from the group consisting of soybean oil, avocado oil, linoleic acid, linolenic acid, and oleic acid. Composition.