KR20210091430A - Cosmetic composition containing natural complex extracts as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for antioxidant, skin whitening, anti-wrinkle or skin inflammatory disease alleviation purposes comprising a natural complex extract as an active ingredient and, more particularly, to a cosmetic composition for antioxidant, skin whitening, anti-wrinkle or skin inflammatory disease alleviation purposes, comprising extracts of Sambucus sieboldiana leaves and Hydrangea macrophylla leaves as active ingredients. The cosmetic composition according to the present invention can exhibit an antioxidant effect that inhibits the activity of DPPH, ABTS, and superoxide anion radicals and a whitening effect through a mechanism of inhibiting the expression of tyrosinase or the like, and can exhibit an anti-inflammatory effect through a mechanism of inhibiting the expression of NO, iNOS, and COX-2.

Description

Cosmetic composition containing natural complex extracts as active ingredient}

The present invention relates to a cosmetic composition for antioxidant, skin whitening, wrinkle improvement or skin inflammatory disease improvement comprising a natural complex extract as an active ingredient. It relates to a cosmetic composition for antioxidation, skin whitening, wrinkle improvement, or skin inflammatory disease improvement.

The proportion of the elderly continues to increase due to the development of medical technology and the extension of life expectancy due to the recent economic development, and chronic inflammatory reactions such as atopy and asthma are increasing due to the chronic inflammatory response due to the immune system abnormality due to the increase in environmental pollution and stress. It is an increasing trend.

In general, the inflammatory response is a defense mechanism of living tissues against external stimuli such as bacterial infection or internal stimuli such as in vivo metabolites. It is caused by the production of cytokines and nitric oxide (NO). In addition, lipopolysaccharide (LPS), also known as endotoxin, is present in the outer membrane of Gram-negative bacteria and induces activation of NF-κB (nuclearfactor-κB), an intracellular transcription factor, in macrophages or mononuclear cells, thereby becoming an inflammatory cytokine , iNOS (inducible nitric oxide synthase), COX2 (cyclooxygenase-2) inducing gene expression and generating mediators of inflammation.

Therefore, in order to control the inflammatory response, iNOS, COX-2, and cytokines and nitric oxide are key factors to control, and substances that control the activity of these factors are attracting attention as preventive and therapeutic agents for inflammatory diseases.

On the other hand, when exposed to ultraviolet rays (UV), reactive oxygen species (ROS) are generated in the skin, and the excess reactive oxygen species causes oxidation, resulting in cell membranes, DNA, and lipids, which are the main components of the skin. It reacts with proteins, polysaccharides and nucleic acids to damage cells and cause skin aging. As a component that inhibits these free radicals, butylated hydroxy anisol (BHA), butylated hydroxy toluene (BHT), and the like are known.

In addition, UV is known to promote melanin production. Melanin is produced through enzymatic and non-enzymatic oxidation reactions of tyrosine in cells called melanocytes present in the basal layer of the epidermis, keratinocytes constituting the epidermis. is transferred to Important enzymes involved in the biosynthesis of melanin include tyrosinase, tyrosinase-related protein 1, and dopachrome tautomerase. Besides tyrosinase, these two enzymes are attracting attention, but the enzyme that plays a crucial role in melanin synthesis is tyrosinase. The first step in the biosynthesis of melanin is induced by tyrosinase. Tyrosine is converted to dopa by tyrosinase, and this dopa is converted to dopa-quinone.

The next steps of these two reactions are known to be possible by non-enzymatic reactions, and it is known that melanin production is possible even with tyrosinase alone.

On the other hand, the substances used for the purpose of anti-inflammatory flufenamic acid (flufenamic acid), ibuprofen (ibuprofen), benzydamine (benzydamine), indomethacin (indomethacin), which are non-steroidal type; Steroids include prednisolone, dexamethasone, hydrocortisone, and betamethasone, but these substances are highly toxic and cause serious side effects such as liver damage, cancer, and stroke. Restrictions follow. In addition, there are cases in which a problem of inducing severe immunosuppression occurs because it cannot selectively act on substances that cause inflammation. Accordingly, the development of anti-inflammatory drugs using various natural products that are safe to the living body and have the advantage of being easy to consume for a long period of time compared to conventional drugs is being developed.

Korea Patent No. 1492074 discloses a composition for skin whitening containing hydrangea extract as an active ingredient, but the development of a component with antioxidant, skin whitening, wrinkle improvement, and anti-inflammatory activity superior to existing compositions is continuously required. It is required to develop a combination or extraction method of various ingredients that can be applied at the same time to exert synergy.

Korean Patent Registration No. 10-1492074

The inventors of the present invention confirmed that a mixture of oleifera leaf extract and hydrangea leaf extract showed excellent effects while conducting research to discover natural extracts for antioxidant, skin whitening, wrinkle improvement, and skin inflammatory disease improvement. was completed.

Accordingly, an object of the present invention is to provide a cosmetic composition for antioxidation, skin whitening, wrinkle improvement, or skin inflammatory disease improvement, comprising an extract of horse chestnut leaf extract and hydrangea leaf extract as active ingredients.

In order to achieve the above object, the present invention provides a cosmetic composition for anti-oxidation, skin whitening, wrinkle improvement or skin inflammatory disease improvement, comprising an extract of horse chestnut leaf extract and hydrangea leaf extract as active ingredients.

In addition, the extract is water, C ₁ ~ C ₄ alcohol, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane or a mixture thereof is used as a solvent may have been extracted.

In addition, the horse pee tree leaf extract and hydrangea leaf extract may be included in a weight ratio of 1: 0.1 to 10.

In addition, the cosmetic composition of the present invention is DPPH (1-1-diphenyl-2-picryl-hydrazyl), ABTS (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid) radical (radical) or superoxide Can inhibit the activity of superoxide anion radical, can inhibit the expression of tyrosinase, elastase, collagenase, NO (nitric oxide), iNOS ( It can inhibit the expression of inducible nitric oxide synthase) or cyclooxygenase-2 (COX-2).

The cosmetic composition according to the present invention can exhibit a whitening effect through an antioxidant effect that inhibits the activity of DPPH, ABTS, and superoxide anion radicals and a mechanism of inhibiting the expression of tyrosinase, etc. An anti-inflammatory effect can be exhibited through a mechanism of suppressing expression.

Therefore, the composition according to the present invention can be usefully used as a material for functional cosmetics for anti-oxidation, whitening, wrinkle improvement, or skin inflammatory disease improvement that does not cause irritation to the skin.

1 is a photograph and graph confirming the effect of the composition of Example 1 on the activity of iNOS and COX-2 through Western blot ((A) iNOS, (B) COX-2).
2 is a photograph and graph confirming the effect of the composition of Example 1 on the activity of iNOS and COX-2 through RT-PCR ((A) iNOS, (B) COX-2).

Hereinafter, the present invention will be described in detail. The terms or words used in the present specification and claims should not be construed as being limited to ordinary or dictionary meanings, and the inventor may properly define the concept of the term in order to best describe his invention. It should be interpreted as meaning and concept consistent with the technical idea of the present invention based on the principle that there is.

The present invention provides a cosmetic composition for antioxidation, skin whitening, wrinkle improvement, or skin inflammatory disease improvement, comprising an extract of horse chestnut tree leaf and hydrangea leaf extract as active ingredients.

Sambucus sieboldiana var. pendula ) is also known as Suap Alley, Ulleung Elderly, or Malophosa, and grows wild in mountain valleys below 900 m above sea level in Ulleungdo, Korea. It is a deciduous shrub, 2 ~ 4 m in height, with cork developed on the stem and hairless on the young branch. The leaves are opposite, and they are radix unilateral, composed of 2-3 pairs of leaflets. The leaf is 10-15cm long and 5-6cm wide, has no hair on the top, and the sawtooth on the edge is bent inward. Flowers bloom from May to June, flowers are yellowish white, and fruits ripen in red in July and contain 3 to 4 seeds.

Hydrangea macrophylla is a deciduous shrub of the dicotyledonous plants Rosales, Hydrangeaceae, and Hydrangea. . In folklore, hydrangea flowers were dried and used as an antipyretic. In addition, hydrangeas are known to have antimalarial, anti-inflammatory, antioxidant, blood pressure lowering, diabetes and diabetic complications prevention effects (Kim, DH, et al., 2017), and the roots of hydrangeas have various autoimmune diseases. It contains halofuginone, which is known as an ingredient that suppresses

The extract in the present invention refers to an active ingredient separated from a natural product, that is, a substance exhibiting a desired activity, and is usually prepared by an extraction process using water, an organic solvent, or a mixed solvent thereof, and water, an organic solvent, or a mixture thereof. It includes an extract of a mixed solvent, a dry powder thereof, or any form formulated using the same. In addition, the extract includes fractionation of the extract that has undergone the extraction process.

The extract is water, C ₁ ~ C ₄ alcohol, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane or a mixture thereof can be used as a solvent and , preferably water, ethanol, 1,3-butylene glycol may be used, and more preferably 50 to 90% by weight of ethanol may be used.

Extraction of the extract may use methods such as immersion extraction, supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, reflux cooling extraction, and ultrasonic extraction.

In particular, the extract can be obtained by immersing the extract after drying 5 to 20 times the amount of the sample using a solvent after drying and crushing the leaves of the hydrangea.

The horse urethra leaf extract and hydrangea leaf extract may be mixed in a weight ratio of 1: 0.1 to 10, preferably in a weight ratio of 1: 0.2 to 5, most preferably 1: 0.5 to 2 It can be mixed by weight ratio.

If the content of the extract is out of the range described above, the synergistic effect of each component may be lowered, so that antioxidant, skin whitening, wrinkle improvement or skin inflammatory disease improvement activity may be reduced.

The cosmetic composition is a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, hair wax, hair aerosol , hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair dye, hair wave agent, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, It may be in the form of hair mousse or hairspray, but is not limited thereto.

The cosmetic composition may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extract.

The cosmetic composition may contain other ingredients that are normally formulated in cosmetics, if necessary. Ingredients that can be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, colorants, fragrances , a blood circulation promoter, a cooling agent, an anti-inflammatory agent, purified water, and the like.

The cosmetic composition may take the form of a solution, emulsion, viscous mixture, and the like, and may include conventional adjuvants and carriers such as stabilizers, solubilizers, vitamins, pigments and fragrances commonly used in cosmetic compositions.

The cosmetic composition may be prepared in any formulation conventionally prepared in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, cosmetic liquid, and the like.

The cosmetic composition of the present invention is DPPH (1-1-diphenyl-2-picryl-hydrazyl), ABTS (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid) radical (radical) or superoxide anion radical (superoxide anion radical) activity can be inhibited, can inhibit the expression of tyrosinase, elastase, collagenase, NO (nitric oxide), iNOS (inducible nitric) oxide synthase) or COX-2 (cyclooxygenase-2)

Hereinafter, examples and experimental examples will be described in detail to describe the present invention in detail. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

<Example 1> Preparation of mixed extract

First, after drying and crushing the leaves of the horse chestnut tree, it was immersed in an ethanol solvent (70% by weight) 10 times the weight of the crushed leaves at room temperature. After immersion for 24 hours, the supernatant was separated using a stirrer (250 rpm) at room temperature, filtered with a filter paper to remove solids, and the dried extract obtained by filtration was concentrated to a high concentration through a rotary vacuum concentrator and concentrated at -70°C. The water was completely evaporated through a freeze dryer by freezing to obtain a horse urinal leaf extract.

After drying and crushing hydrangea leaves, a hydrangea leaf extract was obtained through the same process as above.

Next, the horse urinosa leaf extract and hydrangea leaf extract were mixed in a weight ratio of 1:1 and used as an experimental sample.

<Comparative Example 1>

Only the prepared horse pee tree leaf extract was used as an experimental sample.

<Comparative Example 2>

Only the hydrangea leaf extract prepared above was used as an experimental sample.

<Experimental Example 1> Measurement of antioxidant efficacy

To confirm the antioxidant effect, electron donating ability, ABTS radical scavenging activity, and SOD-like activity were measured.

<Experimental Example 1-1> Measurement of electron donating ability

For the electron donating abilities (EDA) measurement experiment, 60 μl of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 120 μl of an extract with a concentration of 1,000 μg/ml were added, mixed, and left for 15 minutes, and then a microplate reader was used. Absorbance was measured at 517 nm using The electron donating ability was expressed as the absorbance decrease rate of the sample solution and the non-additive group.

The electron donating ability measurement results are shown in Table 1 below.

extract Electron donating ability (%) Example 1 93.5 Comparative Example 1 86.2 Comparative Example 2 89.1

<Experimental Example 1-2> Measurement of ABTS radical scavenging activity

Antioxidant activity measurement using ABTS radicals was carried out by mixing 7 mM 2,2-azino-bis(3-ethyl-benthiazoline-6-sulfonic acid) and 2.45 mM potassium persulfate and storing them at room temperature for 24 hours to form ^{ABTS + .} It was diluted with ethanol, and absorbance was measured at 700 nm by adding 100 μl of a sample having a concentration of 1,000 μg/ml to ^{ABTS + 100 μl in a 1:1 ratio.}

The measurement results of the ABTS radical scavenging ability are shown in Table 2 below.

extract ABTS radical scavenging ability (%) Example 1 94.9 Comparative Example 1 90.0 Comparative Example 2 89.1

<Experimental Example 1-3> Superoxide dismutase (SOD)-like activity measurement

For SOD-like activity, 130 μl of Tris-HCl buffer (50mM Tris-HCl buffer, pH 8.5) and 20 μl of pyrogallol (7.2mM) were mixed in 20 μl of a 1,000 μg/ml sample solution and mixed at 10°C at 37°C. After reacting for a minute, the amount of oxidized pyrrogallol was measured at absorbance at 420 nm using a microplate reader. The SOD-like activity was expressed as the absorbance reduction rate of the samples with and without the addition of the sample solution.

The measurement results of the SOD-like activity are shown in Table 3 below.

extract SOD-like activity (%) Example 1 55.0 Comparative Example 1 45.2 Comparative Example 2 45.5

Through the above experiment, it was confirmed that the composition of Example 1 exhibited superior electron donating ability, ABTS radical scavenging activity, and SOD-like activity, compared to single extracts of the extracts of horse urchin leaf extract and hydrangea leaf extract, thereby exhibiting high antioxidant activity.

<Experimental Example 2> Measurement of skin whitening activity

To confirm the skin whitening activity, tyrosinase inhibitory activity was measured. The reaction zone was 200 U/ml mushroom tyrosinase (Sigma, USA) 40 μl was added and reacted at 37° C. for 10 minutes, and DOPA chrome generated in the reaction solution was measured at 492 nm. The tyrosinase inhibitory activity was expressed as the absorbance reduction rate of the group with and without the sample solution.

The measurement results of the tyrosinase inhibitory activity are shown in Table 4 below.

extract Tyrosinase inhibitory activity (%) Example 1 22.6 Comparative Example 1 8.5 Comparative Example 2 12.7

Through the above experiment, it was confirmed that the composition of Example 1 exhibited superior skin whitening activity compared to the single extracts of the leaf extract and hydrangea leaf extract.

<Experimental Example 3> Measurement of skin wrinkle improvement activity

In order to confirm the skin wrinkle improvement effect, elastase inhibitory activity and collagenase inhibitory activity were measured.

<Experimental Example 3-1> Elastase inhibitory activity

For the measurement of elastase inhibitory activity, 40 μl of the test solution at a concentration of 1,000 μg/ml was taken in a 96-well plate, and 40 μl of porcine pancreas elastase (2.5 U/ml) dissolved in 50 mM tris-HCl buffer (pH8.6) was added. apply For the substrate, 80 μl of N-succinyl-(L-ala)3-p-nitroanilide (0.5 mg/ml) dissolved in 50 mM tris-HCl buffer (pH 8.6) was added and reacted for 30 minutes, and the amount of p-nitroanilide was measured at 445 nm. measured. Elastase inhibitory activity was expressed as a decrease in absorbance in the group with and without the sample solution.

The measurement results of the elastase inhibitory activity are shown in Table 5 below.

extract Elastase inhibitory activity (%) Example 1 13.6 Comparative Example 1 6.6 Comparative Example 2 6.8

<Experimental Example 3-2> Collagenase inhibitory activity

_{Collagenase inhibitory activity measurement was carried out by adding 4mM CaCl 2} to 0.1M tris-HCl buffer (pH7.5), 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (0.3mg/ml) ( Sigma, USA) is dissolved in 125 μl of the substrate solution and 50 μl of the 1,000 μg/ml sample solution is mixed with 75 μl of collagenase (0.2 mg/ml) (Sigma, USA) and left at room temperature for 20 minutes. After that, 250 μl of 6% citric acid was added to stop the reaction, and 1.5 ml of ethyl acetate was added to measure the absorbance at 320 nm. Collagenase inhibitory activity was expressed as a decrease in absorbance in the group with and without the sample solution.

The results of measuring the elastase inhibitory activity are shown in Table 6 below.

extract Collagenase inhibitory activity (%) Example 1 31.4 Comparative Example 1 27.7 Comparative Example 2 20.2

Through the above experiment, it was confirmed that the composition of Example 1 exhibited superior erastase and collagenase inhibitory activity compared to single extracts of horse urinal tree leaf extract and hydrangea leaf extract, thereby exhibiting an excellent skin wrinkle improvement effect.

<Experimental Example 4> Measurement of anti-inflammatory efficacy

In order to confirm the anti-inflammatory effect, the inhibitory effect of NO (nitric oxide), iNOS (inducible nitric oxide synthase) or COX-2 (cyclooxygenase-2) expression was measured.

<Experimental Example 4-1> Measurement of NO production inhibitory activity

The amount of NO produced from RAW 264.7 cells was measured in the form of _{NO 2 present in the cell culture medium using griess reagent.} RAW 264.7 cells were aliquoted to 1×10 ⁵ cells/well in a 96 well plate. 37 ℃, 5% CO ₂ After incubation for 24 hours in an incubator, wash twice with 1X PBS. After treatment with LPS 10μg/ml except for normal, after 2 hours, the sample solution at the concentration of 1,000 μg/ml was treated and cultured for 24 hours to obtain a supernatant. Then, the same amount of griess reagent was added and reacted for 10 minutes in a 96 well plate. After that, the absorbance at 540 nm was measured. The amount of NO produced is shown in Table 7 below,

extract NO production Example 1 59.5 Comparative Example 1 87.4 Comparative Example 2 76.9

<Experimental Example 4-2> Activity of iNOS and COX-2 through Western blot

In order to examine the activity of inflammatory factors iNOS and COX-2, the cell line (RAW 264.7) was cultured for 24 hours after cell seeding in a 100 mm tissue culture dish. After removing the medium, the extract is incubated for 24 to 48 hours in a medium treated with each concentration, and then washed twice with PBS. Radio-immunoprecipitation assay (RIPA) buffer 10ml was dissolved with 100 μl of complete mini 1 tab added, centrifuged at 4℃, 13,200rpm for 20 minutes, and the supernatant obtained by centrifugation was quantified using a BCA protein assay kit. . After separating 20 μl of protein by electrophoresis on 10% SDS-PAGE, the separated protein was transferred to a polyvinylidene fluoride (PVDF) membrane to prepare a blocking buffer (5% skim milk in TBST), and blocking was performed at room temperature for 1 hour. . Dilute the primary antibody and wash 3 times with tris-buffered saline and tween 20 (TBST) every 10 minutes again after over night at 4°C. The secondary antibody was diluted 1:1,000, reacted at room temperature for 2 hours, washed 3 times, and bands were identified and quantified using ^{a Davinch-Chemi TM Imager CAS-400SM instrument.} The experimental results are shown in FIG. 1 .

As shown in FIG. 1 , the protein expression levels of iNOS and COX-2 increased by LPS decreased in a concentration-dependent manner, and inhibitory activities of 24.6% and 56.2% were exhibited at a concentration of 100 μg/ml, respectively. When compared with the control group, vitamin C, iNOS showed a significant result compared to the control group, and COX-2 showed more suppressed protein expression than vitamin C.

<Experimental Example 4-3> Activity of iNOS and COX-2 through RT-PCR

Cell line RAW 264.7 was seeded in a 100 mm culture dish at 1×10 ⁶ cells/well and cultured for 24 hours, then treated with LPS at a concentration of 1 μg/ml for 2 hours, and the extracts were treated by concentration and cultured for 24 hours. After removing the medium supernatant, 1 ml of trizol lysis buffer was dispensed into each well to lyse the cells, and 200 μl of chloroform was dispensed and shaken up and down for 20 seconds. Then, centrifuged at 13,200 rpm for 20 minutes, and the supernatant was transferred to a tube containing 500 μl of isopropanol and mixed. After centrifugation again at 13,200 rpm for 20 minutes, after removing the supernatant, 1 ml of 75% EtOH-diethylpyrocarbonate (DEPC) water was dispensed into each tube and centrifuged at 13,200 rpm for 5 minutes, after which the supernatant was removed and cooled at room temperature. dried. After dissolving 50 μl of distilled water treated with DEPC, 5 μl of RNA and 195 μl of sterile water were added to a 96-well plate, and absorbance was measured at 260 nm and 280 nm, respectively, to measure the total RNA amount. Oligo (dT) 15 primer (500μg/ml) 1μl, extracted RNA (2μg) and nuclease free water _{10μl, react at 75℃ for 5 minutes, 5X reaction buffer, MgCl 2} , PCR nucleotide mix, rnasin inhibitor, Reverse transcriptase and nuclease free water were added and reacted at 25°C for 5 minutes, 42°C for 60 minutes, and at 70°C for 15 minutes to synthesize cDNA.

Polymerase chain reaction (PCR) was performed to examine the mRNA expression of iNOS and COX-2. The primer sequences used in the experiment are shown in Table 8 below. 5X green Go Taq flexi buffer, MgCl ₂ , PCR nucleotide mix (10mM), primer, Go Taq DNA polymerase, nuclease free water, and synthesized cDNA were added to the PCR tube, mixed well, and then PCR was performed. GAPDH, iNOS at 96°C for 2 minutes, 96°C for 10 seconds, 64°C for 30 seconds, 72°C for 1 minute, 72°C for 10 minutes (40 cycles), COX-2 at 96°C for 2 minutes, 94°C 10 sec at 51 °C for 30 sec, 72 °C for 1 min, 72 °C for 10 min (40 cycles), after synthesis by PCR, 1.5% agarose gel with 0.002% ethidium bromide was electrophoresed at 100 V for 40 min. Then, the band was identified using LAS 4,000 and analyzed and quantified.

Gene Primer Sequence (5’ →3’) COX-2 sense GGA GAG ACT ATC AAG ATA GT anti-sense ATG GTC AGT AGA CTT TTA CA iNOS sense AAT GGC AAC ATC AGG TCG GCC ATC ACT anti-sense GCT GTG TGT CAC AGA AGT CTC GAA CTC

The analysis results are shown in FIG. 2 .

As shown in FIG. 2, it was confirmed that the mRNA expression levels of iNOS and COX-2 increased by LPS were decreased, and a significant result was confirmed at a concentration of 100 μg/ml when compared with vitamin C, a control. did.

Through the above experiment, the composition of Example 1 showed superior NO (nitric oxide), iNOS (inducible nitric oxide synthase) or COX-2 (cyclooxygenase-2) expression compared to single extracts of horse urinal tree leaf extract and hydrangea leaf extract. It was confirmed that it exhibited an inhibitory effect and exhibited high anti-inflammatory activity.

Claims (6)

A cosmetic composition for antioxidation, skin whitening, wrinkle improvement, or skin inflammatory disease improvement, characterized in that it comprises a horse urinal leaf extract and hydrangea leaf extract as active ingredients.
According to claim 1,
The extract is _{extracted using water, C 1} to C ₄ alcohol, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane or a mixture thereof as a solvent. A cosmetic composition, characterized in that
According to claim 1,
The extract of the horse pee tree leaf and the hydrangea leaf extract is 1: 0.1 to 10 by weight of the cosmetic composition, characterized in that it is included.
According to claim 1,
The composition is DPPH (1-1-diphenyl-2-picryl-hydrazyl), ABTS (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid) radical (radical) or superoxide anion radical (superoxide anion) radical) a cosmetic composition, characterized in that it inhibits activity.
According to claim 1,
The composition is a cosmetic composition, characterized in that it inhibits the expression of tyrosinase (Tyrosinase), elastase (Elastase), collagenase (Collagenase).
According to claim 1,
The composition is a cosmetic composition, characterized in that it inhibits the expression of NO (nitric oxide), iNOS (inducible nitric oxide synthase) or COX-2 (cyclooxygenase-2).

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