KR20200035746A - Composition for controlling stem blight comprising extracts of Zanthoxylum ailanthoides and Aesculus turbinata - Google Patents

Abstract

The present invention relates to a composition for controlling stem blight, which comprises an extract of Zanthoxylum ailanthoides and Aesculus turbinata as active ingredients, and to a method for controlling stem blight using the same. The extract of Zanthoxylum ailanthoides and Aesculus turbinata as active ingredients is derived from natural products, thereby being harmless to the human body and not causing environmental pollution, and shows control activity against plant diseases of the stem blight in a very small amount, thereby being expected to be useful for the development of eco-friendly natural product sterilizers and the production of high value-added organic products.

Description

Composition for controlling stem blight comprising extracts of Zanthoxylum ailanthoides and Aesculus turbinata}

The present invention is a result of a research conducted by the Ministry of Trade, Industry and Energy and the Korea Institute for Industrial Technology Promotion as an economic cooperation zone industry cultivation project. It's about how to control blight.

Stem blight (canker) is also called bundling and occurs on peach trees, apple trees, and pear trees. The cause of stem blight is parasitism of bacteria such as Diaporthe, Leucostoma, and Endothia belonging to Ascomycetes (dudu type, 1993). It swells, and if it is severe, it may smell alcohol. Small spots of brown or black color are formed in old lesions. If this lesion persists, the branches or stems above the diseased area dry out and die.

The spores of the bacteria that cause stem blight are sprayed when they hit water droplets or rain, and become a new source of infection. Pathogens overwinter in the lesion area and show symptoms in spring or autumn the following year.

Stem blight disease is difficult to control because pathogens remain infected on trees for a long time once parasitic, and useful drugs to control are not yet developed. As a preventive measure, it is recommended that trees are not damaged, but when this disease occurs due to pruning or damage to the East Sea, the affected area is cut with a knife, sterilized with alcohol, and then a thick antiseptic is applied. The dead trees caused by stem blight should be removed and burned or buried, and large trees that are difficult to move should be cut and covered with a non-woven fabric to prevent the spread of pathogens.

Chemical synthetic pesticides have the effect of controlling pests and fungi in the short term, but from a long-term point of view, they cause the resistance of pests and fungi, causing the problem of using more and more toxic pesticides or controlling them several times. In addition, chemical synthetic pesticides are destroying natural food chains, thereby extinguishing microorganisms beneficial to natural enemies and plants. Therefore, it is necessary to develop a safe drug with a side effect of controlling pests and bacteria and having fewer side effects. Accordingly, in recent years, interest in the development of antibacterial agents using natural products has increased.

In this regard, Republic of Korea Patent Registration No. 10-1631825 discloses a composition for controlling stem blight disease, which contains an extract or fraction of an American native ball as an active ingredient, and Republic of Korea Patent Registration No. 10-1800394 contains a seed extract of Lychee Disclosed is a composition for controlling stem blight.

Accordingly, the inventors of the present invention, while developing a composition for the control using natural products, when using a mixture of the extract of the chestnut tree in the conventional extract of mulberry tree, found that the control effect is very excellent even in a very small amount compared to the case of using the conventional mulberry extract alone Then, the present invention has been completed.

Accordingly, one technical problem to be solved in the present invention is to provide an environment-friendly composition for controlling stem blight disease, which exhibits excellent control activity against stem blight in a small amount without being harmless to the human body and causing environmental pollution, derived from natural products. It is to do.

In addition, another technical problem to be solved in the present invention is to provide a method for controlling stem blight using the composition for control.

In one aspect, the present invention provides a composition for controlling stem blight disease, which includes as an active ingredient a oleander and a horse chestnut extract.

One of the active ingredients of the present invention, Zanthoxylum ailanthoides , is a deciduous arboreous tree distributed in Korea, Japan, and China, and grows up to 15m in height, and has small hairs without thorns. The leaves are alternate with feathers, and the small leaves are 19 to 23 thick, broad lanceolate, sharp at the tip, and fine serrated. The flowers are yellowish-white in color and bloom at the end of branches in May in an inflorescence shape and are umbrella-shaped. Fruits are soybeans, ball-shaped, ripe in November, and spicy. The one without thorns is called Zanthoxylum ailanthoides var. Inermis, and the one with red thorns on the thorns is called Zanthoxylum fauriei . Herbs are used as a remedy for colds and school diseases.

In the present invention, the use portion of the mulberry tree can be used as a seed, an outer shell, a leaf, a flower, a root, or a stem, but preferably, the mulberry tree is used.

According to one embodiment of the present invention, as an active ingredient of the composition, a tanabata extract is used in combination with a extract of the syrup tree seed, wherein the weight ratio of the syrup seed extract and the chestnut tree extract is 0.5 to 1.5: 0.5 to 1.5, Preferably 0.8 to 1.2: 0.8 to 1.2, more preferably 1: 1.

The aquilus ( Aesculus turbinata ) is a deciduous tall tree that is planted and raised in sub-central Japan, native to Japan, and the stem reaches 30m in height. The leaves are alternate, and are small leaves with 5 to 7 palm-shaped leaves. The small leaves are long obovate, the largest of which is 15 to 40cm long, 4 to 15cm wide, and have double serrated edges. The back of the leaf has reddish brown soft hairs. The flower runs from May to June in conical shape at the end of the branch, and is reddish white. The inflorescence is 15-25 cm long. Calyx is irregularly divided into 5 branches, and 4 petals. There are 7 surgeries. The fruit is a fruit, and is divided into three. It is sometimes called 'Maronier' in Korea, and saponin obtained from the fruit of the horse chestnut is used as an anti-inflammatory drug. have.

In the present invention, the site of use of the horse chestnut can be used as a seed, an outer shell, a leaf, a flower, a root, or a stem, but preferably, the seed or an outer shell of the hardwood, or a combination thereof.

The term "extract" of the present invention refers to a formulation in which a crude drug is squeezed into an appropriate leach solution and concentrated by evaporating the leach solution, but is not limited thereto, oil, extract, dilution or concentrate of extract, extract obtained by extraction treatment It may be a dried product obtained by drying, a crude product or a purified product thereof.

The extract of the present invention is a seed oil obtained by squeezing a seed, or it can be prepared by using a general extraction method, separation and purification method known in the art for seed, skin, leaf, flower, root or stem. The extraction method may include hot-water extraction, high-temperature extraction, low-temperature extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction, but is not limited thereto.

In addition, the extract may be obtained through a conventional purification process in addition to the method of extracting by the extraction solvent. For example, through various purification methods additionally performed, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity) An extract can also be obtained through the obtained fraction.

According to one embodiment of the present invention, the seed of the mulberry seed can be obtained as a seed oil by compressing the mulberry seed, or the seed is dried and powdered to extract using water, an organic solvent or a mixture thereof. It can be produced by filtering under reduced pressure. At this time, examples of the organic solvent include methanol, ethanol, propanol, butanol, glycerin, ethyl acetate, and nucleic acids, preferably methanol or ethanol, and more preferably ethanol. On the other hand, when water is used as a solvent, it is preferable to prepare the extract after chlorophyll is removed by immersing the mulberry crushed in an acidic solution.

According to a preferred embodiment of the present invention, the seed tree seed extract is seed oil obtained by compressing seed tree seed; Or it can be prepared by washing with a distilled water, and then crushing the seedlings with water or an organic solvent for 65 to 80 hours at 10 to 30 ° C or for 3 to 7 hours at 70 to 110 ° C.

According to one embodiment of the present invention, a horse chestnut extract may be prepared by drying a chestnut seed or an outer shell and then powdering it to extract water, an organic solvent, or a mixture thereof, and then filtering under reduced pressure. At this time, examples of the organic solvent include methanol, ethanol, propanol, butanol, glycerin, ethyl acetate, and nucleic acids, preferably methanol or ethanol, and more preferably ethanol. On the other hand, when water is used as a solvent, it is preferable to prepare an extract after chlorophyll is removed by immersing the powdered chestnut in an acidic solution.

According to a preferred embodiment of the present invention, the horse chestnut extract is washed with distilled water, and then crushed, and then crushed the chestnut seed, the skin, or extracted with water or an organic solvent for 10 to 30 ° C for 65 to 80 hours, or 70 to 70 It can be prepared by extraction at 110 ° C. for 3 to 7 hours.

In the present invention, the "active ingredient" refers to a component that can exhibit the desired activity alone or itself can exhibit activity with an inactive carrier.

In the present invention, the "control" is meant to include the prevention and avoidance of diseases and pests, as well as removal and death. Therefore, even if the plant is already infected with the pathogen, the composition for controlling stem blight disease of the present invention can be applied to remove the pathogen from the host plant, thereby preventing the pathogenesis or exacerbation of the disease.

In the composition of the present invention, the composite extract obtained by mixing the extract of the mulberry tree and the horse chestnut extract is 0.00001 to 15% by weight, more preferably 0.0001 to 10% by weight, and most preferably 0.001 to 5% by weight, based on the total weight of the composition. It can contain as. At this time, when the content of the complex extract is less than 0.00001% by weight, the control effect of the present invention cannot be obtained, and when it exceeds 15% by weight, the effect may not be proportional to the increase in content and thus may be inefficient, and stability of the formulation is secured. There is a problem that is not.

In particular, according to one specific embodiment, the mixed extract of the chestnut tree extract and the horse chestnut extract of the present invention has little or no reduction in the control effect compared to the conventionally available composition for controlling stem blight, even when diluted 500 times or 1000 times. It has excellent stem blight control effect.

The composition for control according to the present invention may be used alone or in the form of a diluent with an inert liquid or a solid carrier, and a surfactant or other auxiliary agent may be used as a drug to which the extract of the mulberry tree and the horse chestnut extract are used alone.

Examples of specific formulations include formulations such as granules, powders, hydrating agents, and emulsions.

Examples of preferred carriers are talc, bentonite, clay, kaolin, diatomaceous earth, white carbon, vermiculite, slaked lime, silica sand, ammonium sulfate, urea, porous solid carriers, water, isopropyl alcohol, xylene, cyclohexanone, methylnaphthalene, alkyl glycol, And liquid carriers such as natural soap. As a surfactant and a dispersing agent, for example, dinaphthylmethane sulfonate, alcohol sulfate ester salt, alkylaryl sulfonate, lignin sulfonate, polyoxyethylene glycol ether, polyoxyethylene alkylaryl ether, polyoxyethylene sorbitan monoal And chelates. Examples of the adjuvant include carboxymethylcellulose, polyethylene glycol, propylene glycol, gum arabic, xanthan gum, and the like, alginic acid, skim milk, and pH buffering agents. In this case, the amount of the mixed extract of the mulberry extract and the horse chestnut extract and / or the amount of the active ingredient thereof, and also the application timing and the applied amount can be appropriately determined based on the effective concentration.

In addition, as one specific example, when the control composition of the present invention is used as an environmentally friendly mist, biodiesel produced based on renewable resources such as vegetable oil or animal fat may be used as a diffusion agent. At this time, the amount of biodiesel used is 50 to 100 times the weight, preferably 60 to 90 times the weight, more preferably 70 to 80 times the weight, even more preferably based on the total weight of the mixed extract of the mulberry extract and the horse chestnut extract. Is used at a weight of 72 to 78 times, even more preferably at a weight of 75 times. If it is used in excess of 100 parts by weight, there is a risk of fire or explosion due to the occurrence of sparks.

In addition, the control composition of the present invention may include other components such as pesticides, other fungicides, herbicides, plant growth regulators, fertilizers, minerals, etc. as necessary as an active ingredient. In addition, the composition for the control of the present invention may contain a plant extract having a control effect as a plant extract of other types other than the extract of the mulberry tree and the horse chestnut extract.

The control composition of the present invention may be used directly as it is or diluted with water or the like. The method for using the control composition of the present invention is not particularly limited, and examples thereof include a method of directly spreading to plants or host plants, a method of irrigating soil, and a method of adding water or fertilizer to plants or soil. . Since the amount of other agents used varies depending on the target disease, target crop, application method, tendency of occurrence, degree of damage, environmental conditions, formulation used, and the like, it is preferable to adjust appropriately.

As a result of diluting the control composition according to the present invention 500 times or 1000 times and treating the bark in the form of a mist, it was confirmed that the present invention showed a control effect equal to or higher in a small amount compared to a conventionally available stem blight control agent. The composition of will be very useful as an environmentally friendly stem blight control agent.

As described above, the composition for controlling stem blight of the present invention, which uses the extract of the mulberry tree and the chestnut tree as an active ingredient, is derived from natural products and is harmless to the human body and does not cause environmental pollution. It is expected to be useful for the development of environmentally friendly natural product sterilizers and production of high value-added organic products because it exhibits control activity.

1 is a view illustrating the control effect of stem blight of a mixed extract of a mulberry extract and a horse chestnut extract according to an embodiment of the present invention.

Hereinafter, examples and the like will be described in detail to help understanding of the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be interpreted as being limited to the following examples. The embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

Example 1: Preparation of mulberry extract

The seeds of the mulberry tree were collected 200 g of samples from Wando Arboretum in Gundo-myeon, Wando-gun, Jeollanam-do, and washed with distilled water three or more times, and then shaded in the dark for 5 days.

Murder tree seed oil was obtained by squeezing the shaded seeds, and the water extracts of the shade tree seeds were crushed using a blender, and then 2 L of water was used for 3 days at 20 ° C and 3 days at 100 ° C, respectively. It was obtained by extracting for a period of time, and the organic solvent extract of seed tree seeds was crushed using a blender, and then ethanol and methanol were extracted for 2 days at 20 ° C for 3 days and at 80 ° C for 6 hours using 2L of ethanol and methanol, respectively. Was obtained.

At this time, during the extraction of hot water, chlorophyll was removed through immersion and decolorization process in 0.1N hydrochloric acid, which is a method for decolorization of chitin, to remove chlorophyll from plants.

The obtained extract was filtered using Whatman filter papers, then concentrated under reduced pressure and freeze-dried with a rotary vacuum evaporator at 50 to 60 ° C to secure a yield.

The extraction yield (%) was calculated according to Equation 1 below.

[Equation 1]

Extraction yield (%) = solid extract (g) / raw material (dry weight) X 100

Each yield of the obtained extracts is as shown in Table 1 below, and the yield of the oil extracted by squeezing the seed of mulberry tree was about 20%, and as a result of extraction using the residue after extraction, the methanol extract was 11.20% at high temperature. The highest yield was shown, followed by 10.87% ethanol extract. It was found that the yield of the mulberry seeds was higher when extracted with an organic solvent than that with water because it contained a large amount of oil.

Example 2: Preparation of horse chestnut extract

The chestnut tree was obtained by obtaining seeds and skins, and washing each sample 200g with distilled water three or more times, and then using it in the dark for 5 days.

The water extract of the chestnut seed and the skin was obtained by crushing the shaded chestnut seed and the skin using a blender, and then extracting them for 4 hours at 20 ° C for 3 days and 100 ° C using 2L of water, respectively, The organic solvent extract was obtained by crushing the shaded chestnut seeds and the outer shell using a blender, and then extracting them using ethanol and methanol 2L for 3 days at 20 ° C and 6 hours at 80 ° C, respectively.

At this time, during the extraction of hot water, chlorophyll was removed through immersion and decolorization in 0.1N hydrochloric acid, which is a method for decolorization of chitin, to remove chlorophyll from plants.

The obtained extract was filtered using Whatman filter papers, then concentrated under reduced pressure and freeze-dried with a rotary vacuum evaporator at 50 to 60 ° C to secure a yield.

Each yield of the obtained extract is as shown in Table 1 below, and the extraction yield of the chestnut seed was highest at 17.05% when extracted with water at high temperature. Then, it was high in order of low temperature water extract and high temperature methanol extract. The extraction yield of the chestnut tree skin was also the highest at 12.11%, and the cold methanol extract and the hot methanol extract were the next highest.

In the case of the chestnut tree, it was judged that the saponin, which is the main component, acted as a surfactant in the aqueous solution state, and the fat-soluble substance was extracted together with the water-soluble substance in the outer skin, as the main component saponin acted as a surfactant in the water extract.

sample Extraction condition Yield (%, w / w) ^a menstruum Temperature (℃) time Seed of a horse chestnut Distilled water 20 3 days 7.23 100 4 hr 17.05 ethanol 20 3 days 1.50 80 6 hr 4.06 Methanol 20 3 days 4.59 80 6 hr 7.07 Horse chestnut Distilled water 20 3 days 5.07 100 4 hr 12.11 ethanol 20 3 days 2.31 80 6 hr 4.50 Methanol 20 3 days 6.63 80 6 hr 6.26 Seed Distilled water 20 3 days 3.76 100 4 hr 9.53 ethanol 20 3 days 7.14 80 6 hr 10.87 Methanol 20 3 days 6.25 80 6 hr 11.20 Seed oil extracted by pressing 18.5

Example 3: Preparation of a control composition according to the invention

Concentrate of ethanol extract of the berry extract of Example 1 prepared in Example 1 and concentrate of hot water extract of the seed and outer skin of the horse chestnut prepared in Example 2 (seed extract: the skin extract is in a weight ratio of 1: 1) 0.01L was mixed with 0.02L of surfactant (Tween-80) in a weight ratio of 1: 1, and then dissolved in 0.96L of water to prepare a 500-fold dilution solution. 10L of water and 20L of biodiesel were mixed with the solution to prepare a spray control composition and used for the antibacterial effect test.

Example 4: Efficacy test of the control composition according to the invention

The control effect on the control composition prepared in Example 3 was tested. The specific test method is as follows.

First, the test medium was PDA (Potato Dextrose Agar, Difco, USA), and the used cherry tree stem blight strain ( Botryosphaeria dothidea ) was pre-sold by the Rural Development Administration Agricultural Genetic Resource Information Center (KACC) (KACC No. 45481) 25 ℃ Incubated for 5 days or more to activate. As a test method, a paper disc method was applied.

The composition prepared in Example 3 was added to each sterilized paper disc (ㆈ 8 mm, manufactured by Advantec) by 50 μl, dried, and then placed on the edge of autoclaved PDA plate medium. The pathogen was inoculated in the center of the PDA medium after taking a disc from the distal end of the mycelium with a colboboro (ㆈ 8 mm). The inoculated strains were cultured in an incubator at 25 ° C for 5 to 6 days, and then the clear zone of the hyphae around the paper disc was examined to control (antifungal) activity. As a control, a disk (disc) dried by absorbing distilled water in the same volume was used together, and a commercially available biodoctor (product of Hyunnong Co., Ltd.) was used in the same manner as above. The results are shown in FIG. 1.

As can be seen in FIG. 1, the mixed extract of the mulberry extract and the horse chestnut extract according to the present invention exhibited a control effect against stem blight, and exhibited excellent control effect even after diluting 1000 times compared to a commercially available product. .

Claims (4)

A composition for controlling stem blight, comprising an extract of Zanthoxylum ailanthoide s and Aesculus turbinata as an active ingredient. [3] The composition for controlling stem blight according to claim 1, wherein the extract of the tree is a seed extract of the tree. [6] The composition for controlling stem blight according to claim 1, wherein the extract of the horse chestnut is a seed, an outer shell, or an extract thereof. Method for controlling stem blight using the composition for controlling stem blight according to any one of claims 1 to 3.

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