KR101874192B1 - OsTat1 gene enhancing plant disease and uses thereof - Google Patents

Abstract

The present invention relates to a gene for OsTat1 ( Oryza sativa tat pathway signal sequence family protein gene) gene and its use for increasing resistance to blight of blight in plant diseases, particularly rice. The rice plant-derived OsTat1 Genetically transformed plants are more resistant to plant diseases than wild-type plants and are therefore effective in increasing crop yields.

Description

OsTat1 gene and its use for increasing plant resistance < RTI ID = 0.0 >

The present invention relates to a plant disease, especially OsTat1 which increases resistance to blight of blight of rice ( Oryza sativa Tat pathway signal sequence family protein gene) gene and its use.

The twin-arginine translocation (TAT) pathway allows proteins in the folded state to move through the energy transfer membrane. In plants, the TAT translocation enzyme is located on the thylakoid membrane of the chloroplast and serves to transport protein into the thylakoid (Sargent et al. 2006). In the chloroplast, the TAT system utilizes close to 50% of the intrillococcal proteins (Schubert et al . 2002). However, there is no study of the correlation between the number of TAT substrates and the number of TAT components (Dilks et al., 2003). The TAT pathway was found in the plant chloroplasts, but most of the studies have been focused on the TAT of bacteria, and limited research has been done on the plants, and no studies have been conducted on rice.

Rice is one of the most economically important food crops. Blight of rice blight is a bacterial disease caused by rice blast fungus (Xanthomonas oryzae pv. Oryzae), which is a pathogenic bacterium of rice blight, and occurs mainly on the leaves. Damage pattern is caused by loss of photosynthetic rate And quality degradation. Blight of rice blight occurs in most rice cultivation areas including Asia, Africa, Latin America and Australia as well as Korea, and is known to be a serious disease particularly in Southeast Asia. Currently, a pathogenicity test for rice varieties is conducted for the identification of races that refer to different types or variants in the same plant pathogenic bacterium. In Korea, it is reported that there are 5 races (K1, K2, K3, K4, K5) as a result of testing the race by substituting the discriminated cultivars of Japan. Currently, the disease occurrence and the disease occurrence through the identification of the presence of pathogenic bacteria in the rice blast in the rice growing area are analyzed through the density analysis of bacteria in the nonsense or plant, but this method requires much time and effort. Therefore, it is very important to develop a method for detecting pathogenic fungus on rice blast in rice or a rice plant to which the resistance of the pathogen has been imparted.

Accordingly, the inventors of the present invention have found that Xanthomonas oryzae pv oryzae) and coupled OsTat1 (Oryza sativa (Os) twin -arginine translocation) was cloned path signal protein which, by inserting the gene into the wild-type dongjinbyeo having a sensitivity of cDNA of the protein in rice huinip blight was produced overexpression body , And rice paddy having resistance to blight of blight of white blossom was produced, thereby completing the present invention.

Application No. 10-2012-0099311

It is an object of the present invention to provide an OsTat1 ( Oryza sativa twin-arginine translocation 1) gene derived from rice having resistance to blight of blight, a recombinant comprising said gene, and a host cell transformed with said vector.

Another object of the present invention is to provide a method for producing a compound of formula And to provide a transgenic plant that is resistant to plant-borne transgenic plants.

It is still another object of the present invention to provide a method for producing a transgenic plant.

According to one embodiment of the present invention, OsTat1 ( Oryza sativa twin-arginine translocation 1) gene derived from rice having resistance to blight of blight of blight is provided.

According to another embodiment of the present invention, the present invention is characterized in that the gene comprises SEQ ID NO: 1. As used herein, the term "gene" may be used in an equivalent meaning to "polynucleotide ". The term "polynucleotide" is used herein in its equivalent sense to the terms "polynucleotide molecule", "polynucleotide sequence", "nucleic acid", "nucleic acid molecule", "nucleic acid sequence" acid or RNA (ribonucleic acid).

According to another aspect of the present invention, there is provided a recombinant expression vector comprising OsTat1 ( Oryza sativa twin-arginine translocation 1) gene. Said vector comprising 1) a polynucleotide encoding said gene, 1) a polynucleotide of 1) operably linked to induce expression of said polynucleotide, 2) an expression control sequence; And 3) a transcription termination sequence.

As used herein, the term "expression control sequence" is used interchangeably with the terms "expression regulator "," promoter ", and the sequence includes a regulatory nucleic acid ≪ / RTI >

As used herein, the term "operatively linked" means functionally associated with the expression control sequence of a DNA sequence, whereby the expression of the DNA sequence is regulated by the expression control sequence.

The promoter in the recombinant expression vector of the present invention is preferably a constitutive promoter that is always active in at least one cell, tissue or organ under most environmental conditions and during most of the growth and development of the plant, And the like. Examples of the constant promoter include actin, HMGP, CaMV 35S, CaMV 19S, GOS2, ubiquitin, rice cyclopylline, corn H3 histone, alfalfa H3 histone, actin 2, 34S FMV, Rubisco small subunit, OCS, But are not limited to, the promoters of SAD1, SAD2, or nos. In addition, the recombinant vector of the present invention can use a ubiquitous promoter, an inducible promoter, an organ or tissue specific promoter, and the contents of such a promoter are known to those skilled in the art.

The term "transcription termination " as used herein refers to a regulatory sequence that is a DNA sequence at the 3 'end of the primary transcript and at the end of the transcription unit that is a signal for polyadenylation and transcription termination.

The recombinant vector of the present invention may contain a selectable marker for identification and selection of transformed cells, for example, a gene showing resistance to an antibiotic or herbicide. The selectable markers include antibiotics such as nptII which phosphorylates neomycin and kanamycin, hpt which phosphorylates hygromycin, bleomycin, streptomycin, tetracycline, chloramphenicol, ampicillin, gentamycin, geneticin (G418) , Spectinomycin, blasticidin and resistance genes for herbicides, for example bar which provides resistance to Basta ™; AroA or gox, which provides resistance to glyphosate, or a gene that provides resistance to imidazolinone, phosphinotricin, or sulfonylurea, or a metabolic trait that causes the plant to nose only with a carbon source Such as the manA gene or the xylose isomerase for use of xylose, or a reflective quantitative marker such as resistance to 2-deoxyglucose. In addition, X-Gal decomposition by a beta-glucuronidase, GUS, or a substrate to be developed, for example, beta-galactosidase, as a visible marker gene; As the luminescence, luciferin / luciferase system or fluorescence green fluorescence protein, GFP and derivatives thereof can be mentioned.

The recombinant expression vector of the present invention may contain a replication origin for replication and maintenance in a host cell.

According to another aspect of the present invention, there is provided a transformed host cell comprising the recombinant expression vector of the present invention. According to a preferred embodiment of the present invention, the transformed host cell is a bacterial cell or a plant cell, and the bacterial cell is preferably an Agrobacterium cell, and the plant cell may be a monocot plant cell or a dicot plant cell.

According to another aspect of the present invention, there is provided a transgenic plant having resistance to a plant disease, which is transformed with OsTat1 ( Oryza sativa twin-arginine translocation 1) gene and overexpressing the gene .

In the present invention, the terms "transfection" and "introduction" are used interchangeably and include the transfer of an exogenous polynucleotide to a host cell regardless of the method used. The plant tissue that can be used for transformation using the recombinant vector of the present invention is a tissue capable of cloning propagation by organogenesis or embryogenesis and allowing the entire plant to be regenerated from the tissue. The particular tissue used for the transformation will be available in a particular species to be transformed and will be selected according to the most suitable clonal propagation system. For example, a typical transgenic target tissue can be a leaf disc, a flowerpot, a pear, a cotyledon, a hypocotyl, a large gamet, a callus tissue, a fissured tissue (e.g., apical mitotic tissue, For example, cotyledonary and hypocotyledonous tissue).

In the present invention, Agrobacterium tumefaciens-mediated transformation can be used.

According to a preferred embodiment of the present invention, the transformed plant of the present invention is a monocotyledonous plant or a dicotyledonous plant. Preferably, the transgenic plant may be any plant selected from the group consisting of rice, wheat, barley, corn, tobacco, potato, tomato and sweet potato.

As used herein, the term "plant disease" includes all diseases that can occur in a plant, and more specifically, a disease caused by a disease of rice blast, rice blast, rice sheath blight, tomato gray mold, tomato blight, And pepper anthracnose. Examples of causative agents include Magnaporthe oryzae, Thanatephorus cucumeris, Botrytis cinerea, Phytophthora infestans, ), Puccinia recondita, Blumeria graminis f.sp. hordei and Colletotrichum coccodes, and preferably one species selected from the group consisting of May be Xanthomonas oryzae pv. Oryzae, which is a wilt of rice blast.

 According to another aspect of the invention, the present invention provides a method for producing a transgenic plant having resistance to a plant disease comprising the steps of:

(a) (i) a polynucleotide encoding OsTat1 ( Oryza sativa twin-arginine translocation 1) gene of SEQ ID NO: 1; (Ii) an expression control sequence operatively linked to the polynucleotide of (i) to induce expression of the polynucleotide; And (iii) producing a recombinant expression vector comprising a transcription termination sequence;

(b) using the recombinant expression vector of step (a) OsTat1Into a plant cell in a form capable of expressing the polynucleotide; And

(c) culturing the plant cell into which the polynucleotide encoding OsTat1 of step (b) has been introduced in a form capable of expression.

According to another aspect of the present invention, the present invention provides a method for imparting resistance to a plant disease in a plant comprising the steps of:

(a) (i) a polynucleotide encoding the gene of SEQ ID NO: 1; (Ii) an expression control sequence operatively linked to the polynucleotide of (i) to induce expression of the polynucleotide; And (iii) producing a recombinant expression vector comprising a transcription termination sequence; And

(b) introducing the OsTat1 coding polynucleotide into a plant cell in an expressible form using the recombinant expression vector of step (a).

The advantages and features of the present invention are summarized as follows:

(i) The present invention relates to a rice-derived OsTat1 gene capable of imparting resistance to plant diseases in plants and a use thereof.

( Ii ) Transgenic plants in which the rice-derived OsTat1 of the present invention was introduced into plants exhibited increased resistance to plant diseases, particularly, dry leafy blight .

Iii ) Therefore, the OsTat1 of the present invention can be used in plants, especially crops, for the purpose of enhancing plant resistance and increasing yield.

The rice plant-derived OsTat1 Genetically transformed plants are more resistant to plant diseases than wild-type plants and are therefore effective in increasing crop yields.

FIG. 1 shows the result of analysis of the amino acid sequence of the TAT pathway signal protein in different plant species. FIG.
FIG. 2 shows the phylogenetic relationship of the TAT pathway signal protein group in plants. FIG.
FIG. 3 is a diagram showing the vector schematic diagram and the transformed colonies of the present invention (A: a schematic diagram of vector preparation of LOC_Os01g45640.1 ( OsTat1 ) inserted in pCAMBIA1300; B: transformation of pCAMBIA1300 :: OsTat1 into Agrobacterium strain EHA105 Afterwards, confirmed by PCR)
FIG. 4 is a graph showing the results of TaqMan qRT-PCR of the OsTat1 overexpressant of the present invention (NC: negative control; PC: positive control).
FIG. 5 is a graph showing changes in OsTat1 mRNA expression in rice (A), growth stage (B), Xoo (C) after inoculation.
6 is a first copy (copy) OsTat1-appeared after disease progression, huinip blight strains over-expressing plant inoculation 16 days of disease progression aspect of huinip blight in overexpression plants (A), huinip blight inoculation 16 days later, the first copy OsTat1 Fig.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

[ Example One] OsTat1 Isolation of gene and gene sequence analysis

Total RNA was isolated from young leaves of Japonica rice cultivar Jinbabi rice using RNAiso Plus (Takara Bio Inc. Tokyo, Japan), and 1 μg of total RNA was extracted with 20 μl of cDNA synthesis kit (Invitrogen, Carlsbad, CA, USA) Were synthesized.

The target gene OsTat1 , and the following primers were used (Fw: ATGGTAATCACGGCTCTGCAGCT, Rv: GTCGACTCAAATGGTAGCCTCGACCAATTTA).

The PCR conditions were denaturation at 95 ° C. for 5 minutes, denaturation at 95 ° C. for 30 seconds, annealing at 55 ° C. for 30 seconds, extension at 72 ° C. for 2 minutes, and finally extension at 72 ° C. for 10 minutes Respectively. After PCR, the PCR products were confirmed by electrophoresis on 1% (w / v) agarose gel and the bands were separated using gel purification kit (Bioneer, CA, USA). The isolated PCR products were cloned into pGEMT-easy vector (Promega, Madison, USA) and then subjected to sequence analysis by Macrogen (Macrogen, Seoul, Korea). Sequence homology was verified using hierarchical clustering and multiple sequence alignment.

2. Analysis using web-based tools

In addition, the target gene sequence was verified using the BLAST program of NCBI. Assay of the assay of OsTat1 and other TAT genes of the present invention was performed by the ClustalX program (Thompson et al., 1997). The number of trees was created by neighbor-joining (NJ) method and 1000 bootstrap MEGA program.

The presence of N-terminal signal peptides was detected using iPSORT software (Bannai et al. 2002) and TMHMM Server v. 2.0.

3. Results

The sequence and systematic analysis results of OsTatl are shown in Fig. 1 and Fig.

As shown in FIGS. 1 and 2, LOC_Os01g36294 has an ORF (open reading frame) of 393 bp, which encodes 131 amino acids and predicts the molecular weight of 14.3899 kDa and theoretically has an isoelectric point of 11.208. Clustering sort result of the protein sequence, 4538.ORGLA01G0196500.1 (Oryza glaberrima, 99.23% ) and 4533.OB01G33640.1 (Oryza Brachyantha , 74.61%) and OsTat1 . A close homology relationship is Brachypodium 15368.BRADI2G45030.1 (59.37%) and 15368.BRADI2G45040.1 (50.47) of distachyon were found in distant species.

In addition, amino acid sequence inferred from phylogenetic analysis indicates that it appears to be a lineage between monocotyledonous and dicotyledonous plants. Specifically, the two subgroups were formed as monocotyledons, and one group was OSJ _02797 (japonica rice, OsTat1 ), OSI_03044 (Indica rice), OB01G33640 ( O. brachyantha ), ORGLA01G196500.1 ( O. glaberrima ) As shown in Fig. The other groups were composed of SI003328M (S. italica), SB03G029370.1 (S. bicolor), and GRMZM2G067583 (Z. mays) containing a coiled-coil domain similar protein in the dicotyledonous plant population.

Also, as a result of TTHMM analysis, it was predicted that the entire nucleotide sequence had no outer membrane spiral, and the above prediction was expected as a result of the number of four AAs in the perforation helix. According to iPSORT prediction, OsTat1 was confirmed to be a chloroplast transport peptide .

[ Example 2] OsTat1 of  Including vectors and transformants production

1. Preparation of vector and transformants

OsTat1 For gene vector preparation and transformant preparation, isolated The BamHI and SalI sites of pCAMBIA1300 (see FIG. 3A), which is a binary vector containing OsTat1 cDNA and including a systemic expression promoter, and kanamycin as a bacterial selection marker and hygromycin as a plant selection marker, Respectively. The above vector was added to EHA105 (Hood et al. 1993) of Agrobacterium and cultured in AB solid medium containing 50 mg / L of kanamycin for 3 days at 28 ° C with cancer treatment. Using the AAM medium, OD ₆₀₀ A 0.01-fold suspension of Agrobacterium was prepared, and the callus cultured for 10 days with Dong-Jin rice was removed. The callus was then inverted gently for 3 minutes in a suspension, and then the callus was dried. The transformed calli were inoculated on 2N6-AS medium containing 0.4% agar for plant culture and co-cultured for 2 days at 25 ° C in dark. Thereafter, the calli co-cultured in a medium containing 500 mg / L of carbenicillin was washed and then dried to prepare 2N6 medium containing 50 mg / L hygromycin and 400 mg / L carbenicillin And cultured at 32 ° C for 2 weeks in a continuous light condition.

Subsequently, calli were induced in regeneration medium in MSR medium, and then the plant grown as a plant was transplanted into sterilized culture soil and transferred to a greenhouse after 2 weeks of purification, to produce transgenic rice. The results of PCR for the vector construct and the transformed colonies are shown in FIG.

2. Check for Transformation

NanoDrop-1000 (NanoDrop Technologies) was used to measure purity and concentration after extracting the nuclear DNA of the transgenic rice, and PCR was performed to confirm the transformation. PCR was performed using an OsTat specific primer and Takara ExTaq (Takara, Shiga, Japan). The PCR conditions were as follows. Denaturation at 95 ° C for 5 minutes, followed by 35 cycles of denaturation at 95 ° C for 30 seconds, annealing at 55 ° C for 30 seconds, and extension at 72 ° C for 30 seconds. Finally, extension at 72 ° C for 5 minutes was performed. After PCR, the PCR product was confirmed by electrophoresis on 1% (w / v) agarose gel.

3. Rice Transformants Number of copies  Confirm

In order to determine the copy number of a rice plant transformant, and performing ^{TaqMan ® (Applied Biosystems, CA USA} ) analysis. The analysis included 10 ng of nuclear DNA in the T ₀ plant, two primers to sense the target sequence and an MGB probe labeled with FAM ^™ , two primers to detect the standard sequence and a TAMRA ^™ probe labeled with VIC ^® , AmpliTaq Gold ^® DNA polymerisation A TaqMan genotyping assay master mix containing the enzyme was used. The total qPCR reaction was repeated twice and analyzed using an ABI 7900HT (Applied Biosystems, CA USA) instrument. Reaction conditions were: enzyme activity at 95 ℃ for 10 min, denaturation at 95 ℃ for 15 sec and annealing at 60 ℃ for 60 sec. The data files were then copied using the CopyCaller ^® program. The number of copies of the target sequence was determined by the relative amount of each experiment (RQ), which was compared by the C _T (ΔΔC _T ) method (after calculating the C _T difference (ΔC _T ) between the target sequence and the standard sequence And comparing the ΔC _T values to determine copying of the remaining samples based on 2 copies of the target sequence). The results are shown in Fig.

As shown in Fig. 4, a transformation method using embryogenic callus and Agrobacterium generated in rice was used, and then the generation was advanced. The copy number of the transformant cultivated in the greenhouse was confirmed by TaqMan qRT-PCR. Based on the results, 5 independent single copy individuals were isolated and the phenotype was analyzed using the separated individuals.

4. OsTat1  Identification of gene expression and gene expression

In order to confirm the expression level of OsTat1 , total RNAs were extracted according to the difference of each organ, growth stage, and inoculation time (using Dongjinbang used as a resistant strain, Jinbai rice and transforming material) Respectively.

More specifically, the rice organs collected roots, stems, leaf sheaths, leaves and leaflets. Samples were collected at germinated seeds, 3 - leaf stage, top - seeding stage and heading stage and RNA was extracted using RNeasy Mini Kit (Qiagen). Total RNA was purified using DNase 1 kit (Invitrogen). SuperScript ^TM III Reverse Transcriptase (Invitrogen) containing oligo (dT) ₂₀ primer was used for cDNA synthesis. OsTat1- specific internal primers were prepared and used for RT-PCR, and the control was loaded using ubiquitin primer. The internal control was also standardized to confirm the quantitative results of the RT-PCR reaction. The results are shown in Fig.

As shown in Fig. 5, The OsTat1 gene was confirmed to exhibit organ specific expression. Especially, high expression level was observed in the leaves, and it was confirmed that the expression level was low in the leaf, leaf sheath, stem, and root (FIG. 5A). The expression level of OsTat1 was particularly high in the early stage of growth (germination, third leaf stage and top seedling stage), and it was confirmed that expression level was small in early stage (FIG. 5B).

In addition, OsTat1 showed higher expression levels in resistant cultivars at 4 hours and 8 hours after inoculation with P. aeruginosa , although the expression pattern did not agree with time - sensitive differences between susceptible and resistant cultivars. The peak at 4 hours after inoculation increased more than twice as compared with the blight susceptible varieties, and was continuously decreased from 8 hours to 48 hours (Fig. 5C).

[ Example  3] Confirmation of the resistance of rice blight to transgenic plants of the present invention

T _o a transgenic plant produced by the invention is the forward purified in a greenhouse, and 28 days after the lapse of the plant was transplanted into the test package. Plants were grown for 8 weeks prior to inoculation. Suspensions for inoculation were grown in PSA (peptone sucrose agar) medium for 48 hours with Xanthomonas oryzae pv. oryzae (Korean isolate K2) was prepared by calibrating to 1 x 10 ⁸ cfu / ml. Inoculation of the plant bacterium was performed by leaf cutting method. Five leaves per plant were inoculated and the length from the section cut at 8, 12 and 16 days after inoculation was measured. The mean values were interpreted using a standard evaluation system (SES IRRI, 1996). In the control plants, "Dongjinnae" (susceptible), resistant varieties "Jinbukbyeo" and transgenic plants were used. The results are shown in Table 1 and Fig.

As shown in Figure 6, the transformed plant is OsTat1 Xoo (Xanthomonas oryzae pv. Oryzae Strain K2) (Fig. 6A). Resistance was found to be significantly better in the transgenic lines and in the resistant varieties. The five T ₀ transgenic plants were found to have significantly reduced lengths of symptoms when compared to wild-type strain (19 cm) and control plants (13.5 cm). The four plants (OsTat1-7, OsTat1-13, -14 and -17) showed high resistance and the length of the symptom ranged from 1.9 cm to 4.4 cm. One plant (OsTat1-3) had moderate resistance (9.7 cm ) (Table 1, 6B and 6C).

Lines Lesion (cm) 16DAI SES (Rating) OsTAT1-3 9.7 ± 0.76 MR OsTAT1-7 4.2 ± 1.15 R OsTAT1-13 4.4 ± 0.94 R OsTAT1-14 1.9 ± 1.36 R OsTAT1-17 3.4 ± 2.90 R Dong Jin (S) 19.0 + - 1.66 S Jinbag (R) 1.0 + 0.04 R Mock 13.5 ± 0.32 MS

<110> Chungbuk university industry cooperation <120> OsTat1 gene enhancing plant disease and uses thereof <130> P-354 <160> 2 <170> KoPatentin 3.0 <210> 1 <211> 393 <212> DNA <213> Artificial Sequence <220> <223> OsTat1 cDNA sequence <400> 1 atggtaatca cggctctgca gcttctccct ccaatccttg gccttccggc gacggcgaga 60 aatggctacg ccgtcagaag gccacggatg atgactgtga cttgctgcag gcacaaccag 120 gcaaccactg tacatgaatc aaggctaaca agcagtttat cccgtagaga tgcactgtca 180 tacatgtcgt ctgcgttcat agcaacgctt ctggtggctg gtcctgcaga agcaagaacc 240 tccaggcaag agaacaagag aaaagtaagg gagaagctgg agaagctccg ggagaaggca 300 ctaggcccag atgataaaaa tggagccatc cgtaagaagg aatctttggc gaacttgttg 360 attcctccta aattggtcga ggctaccatt tga 393 <210> 2 <211> 131 <212> PRT <213> Artificial Sequence <220> <223> OsTat1 protein sequence <400> 2 Met Val Ile Thr Ala Leu Gln Leu Leu Pro Ile Leu Gly Leu Pro   1 5 10 15 Ala Thr Ala Arg Asn Gly Tyr Ala Val Arg Arg Pro Arg Met Met Thr              20 25 30 Val Thr Cys Cys Arg His Asn Gln Ala Thr Thr Val His Glu Ser Arg          35 40 45 Leu Thr Ser Ser Leu Ser Ser Arg Asp Ala Leu Ser Tyr Met Ser Ser      50 55 60 Ala Phe Ile Ala Thr Leu Leu Val Ala Gly Pro Ala Glu Ala Arg Thr  65 70 75 80 Ser Arg Gln Glu Asn Lys Arg Lys Val Arg Glu Lys Leu Glu Lys Leu                  85 90 95 Arg Glu Lys Ala Leu Gly Pro Asp Asp Lys Asn Gly Ala Ile Arg Lys             100 105 110 Lys Glu Ser Leu Ala Asn Leu Leu Ile Pro Pro Lys Leu Val Glu Ala         115 120 125 Thr Ile ***     130

Claims (10)

OsTat1 ( Oryza sativa twin-arginine translocation 1) gene consisting of the nucleotide sequence of SEQ ID NO: 1 derived from rice having resistance to blight of blight. delete A recombinant vector comprising the gene of claim 1. A transformed host cell comprising the recombinant expression vector of claim 3. 5. The host cell according to claim 4, wherein the transformed host cell is a bacterial cell or a plant cell. A transgenic plant having resistance to blight of blight with OsTat1 ( Oryza sativa twin-arginine translocation 1) gene comprising the nucleotide sequence of SEQ ID NO: 1 of claim 1. 7. The transgenic plant according to claim 6, wherein the transformed plant is a monocotyledonous plant or a dicotyledonous plant. [8] The transgenic plant according to claim 7, wherein the transgenic plant is any one selected from the group consisting of rice, wheat, barley, corn, tobacco, potato, tomato and sweet potato. delete A method for producing a transgenic plant having resistance to blight of blight comprising the steps of:
(a) an OsTat1 (Oryza sativa twin-arginine translocation 1) gene comprising the nucleotide sequence of SEQ ID NO: 1 derived from rice having the blight resistance to the blight of Paragraph 1; (Ii) an expression control sequence operatively linked to the gene of (i) to induce expression of the gene; And (iii) producing a recombinant expression vector comprising a transcription termination sequence;
(b) introducing into a plant cell a polynucleotide encoding OsTat1 (Oryza sativa twin-arginine translocation 1) gene comprising the nucleotide sequence of SEQ ID NO: 1 using the recombinant expression vector of step (a) ; And
(c) culturing a plant cell into which a polynucleotide encoding OsTat1 (Oryza sativa twin-arginine translocation 1) gene consisting of the nucleotide sequence of SEQ ID NO: 1 in step (b) is introduced.

Images