KR101806007B1 - Manufacturing method of cosmetic composition containing nano particle natural materials and cosmetic composition manufactured by the same - Google Patents

Abstract

The present invention relates to a method for preparing a cosmetic composition containing a nanoized natural component and a cosmetic composition prepared by the method. More particularly, the present invention relates to a cosmetic composition comprising a red ginseng extract and a sea cucumber nano extract, To prepare a culture solution, a centrifugation step of centrifuging the culture solution prepared through the culture solution production step, and a stabilization step of stabilizing the separated mixture through the centrifugation step.
The cosmetic composition containing the nano-natural ingredients produced through the above-mentioned process is nano-active, and has high skin absorption power, excellent moisturizing, whitening and antioxidative effect, and also has a low skin irritation due to natural ingredients.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for producing a cosmetic composition containing a nano-structured natural component, and a cosmetic composition prepared by the method. [0002]

The present invention relates to a method for producing a cosmetic composition containing a nanoized natural component and a cosmetic composition prepared by the method. More particularly, the present invention relates to a cosmetic composition comprising a nano- In addition, the present invention relates to a process for preparing a cosmetic composition containing a nano-natural ingredient, which is composed of a natural ingredient and exhibits a low skin irritation effect, and a cosmetic composition prepared by the process.

Cosmetics is a product that meets the fundamental desire of human beings who want to be more beautiful, and has been used historically since prehistoric times, and the frequency of use has been increasing over time.

In contemporary society, the development of industry has brought about environmental problems as a side effect as opposed to the enhancement of human life. Air pollution, water pollution, and the increase in ultraviolet rays caused by global warming have an adverse effect on the skin, which means that more time is spent in managing the skin.

In addition, modern society has become more interested in not only skin beauty but also wellness and LOHAS. Therefore, functional cosmetics that go beyond ordinary skin cosmetics have become a preference for highly functional beauty healthcare products made of raw materials of natural materials. More.

Skin is the outermost organ of the human body and acts as a primary defense to protect the body, protecting the body from environmental factors harmful to the human body such as environmental pollutants and pathogens. The stratum corneum, which is the most out of the skin, plays a role in maintaining skin balance by participating in water evaporation and absorption of the skin. It consists of keratinocite and intercelluler lipid, and it is made of lamellar form. The moisture content in the stratum corneum is closely related to the health of the skin. In normal healthy skin, it contains about 20% of the water, and this moisture content is a main factor of skin elasticity, flexibility and suppleness. More specifically, the functions of the stratum corneum include transdermal absorption, water content, and outermost horny layer excretion, and the role of intercellular lipids is known to play an important role.

Skin of the human body can easily cope with external stimuli such as ultraviolet rays at various growth stages. However, as the age increases, the defensive ability is lowered and oxidation of the lipid, protein oxidation, chain break of collagen and elastin as elastic fibers and abnormal cross Binding, breakage of the hyaluronic acid chain, promotion of melanin production reaction, and damage of the DNA. As a result, the skin is affected by defective skin, loss of skin elasticity, roughness of skin, wrinkles, thickening of skin, And dryness of the skin.

Therefore, in order to prevent skin aging, it is necessary to develop an antioxidant system and a skin soothing serum which can protect skin from ultraviolet rays, inhibit the production of active oxygen, and remove the generated active oxygen.

Saponin of Red Ginseng Extract is composed of more than 30 kinds of ginsenosides. Ginseng is processed by steam several times to produce red ginseng containing ginsenoside which is useful for human body. have. Studies on skin cosmetics of red ginseng have shown that ginsenoside-F1 not only reduces cell death due to ultraviolet irradiation in human keratinocytes, but also reports keratinocytes from apoptosis induced by ultraviolet rays [J . Invest. Dermatol., 121, 607-613, 2003] Ginsenoside-Rb2 has been reported to have proliferative effects on skin cells (Arch. Pharm. Res., 25, 71-76, 2002]. Compound K also promotes hyaluronan synthase 2 (HAS2) gene expression associated with hyaluronan biosynthesis, which is known to be one of skin elasticity components in human keratinocytes [Biochem. 316, 348-355, 2004]. Recently, it has been reported that oral administration of red ginseng and soybean extract to hairless mice has the effect of preventing wrinkle formation. [Korean J. Food Sci. Technol., 37, 986-996, 2005]. These ginsenosides are the major components of red ginseng extract, and are the main ingredients of red ginseng extract. They are the main components of red ginseng extract. They are anti-inflammatory, anti-allergic, anti-cancer effect, erectile effect, blood pressure lowering effect, anti-cholesterol effect, anti- Therapeutic effects have been reported. [Biol. Pharm. Bull, 32 (9), 1552-1558, 2009].

On the other hand, Sea Cucumber Extract is a generic term for sea cucumbers belonging to the echinoderms of echinoderms. It is called "sea gins" or similar to cucumber, meaning "ginseng" It is also called 'sea cucumber' in the West.

Sea cucumbers are rarely alkaline foods and contain a lot of minerals such as calcium, iron, phosphorus and iodine. The calcium and iron content is so high that it is known to aid in the hematopoiesis of the young and pregnant women in the growing season. It is found that the amount of phosphorus is in an ideal ratio to help absorption of calcium and it is found as a good source of calcium source. It also contains chondroitin sulphate, which prevents aging of skin and blood vessels. It is also known as a low-calorie food for preventing obesity and adult diseases.

Studies on sea cucumber have been actively carried out so far. However, studies on the fermented sea cucumber extract which can stabilize the natural materials while maximizing the active ingredients through fermentation process are insufficient.

Accordingly, the present invention relates to a cosmetic composition for enhancing the content of active ingredients including fermented sea cucumber extract and various functional herbal ingredients through a complex fermentation process. The present invention relates to a cosmetic composition comprising a fermented sea skin extract, Wrinkle improvement, anti-atopy and anti-inflammation.

Korean Patent Registration No. 10-1318115 (Oct. Korean Patent Publication No. 10-2016-0009928 (2016.01.27).

The object of the present invention is to provide a method for producing a cosmetic composition containing a nano-sized natural component, which has a nano-sized active ingredient and is high in skin absorbency, excellent in moisturizing, whitening and antioxidative activity, And to provide a cosmetic composition which is produced by a manufacturing method.

The object of the present invention is to provide a method for preparing a culture solution for producing a culture solution by inoculating Bacillus subtilis, lactic acid bacteria and yeast into a mixture containing a red ginseng nano extract and a sea cucumber nano extract, a centrifuging step of centrifuging the culture solution prepared through the above- And a stabilization step of stabilizing the separated mixture through the centrifugal separation step. The present invention also provides a method for producing a cosmetic composition containing a nano-natural ingredient.

According to a preferred feature of the present invention, between the centrifugal separation step and the stabilization step, 0.01 to 0.5 parts by weight of germanium aqueous solution and 0.5 to 10 parts by weight of sulfur aqueous solution are added to 100 parts by weight of the culture liquid separated through the centrifugal separation step, It is assumed that the step further proceeds.

According to a more preferred feature of the present invention, in the step of preparing the culture liquid, 0.05 to 1 part by weight of Bacillus subtilis, 0.05 to 1 part by weight of lactic acid bacterium and 0.05 to 1 part by weight of yeast are inoculated in 100 parts by weight of a mixture containing red ginseng nano extract and sea cucumber nano extract And culturing at a temperature of 20 to 30 DEG C for 20 to 30 hours.

According to a further preferred feature of the present invention, the mixture comprises 100 parts by weight of purified water, 1 to 5 parts by weight of red ginseng nano extract, and 1 to 5 parts by weight of sea cucumber nano extract.

According to still another more preferred characteristic of the present invention, the red ginseng nano extract is prepared by adding 100 parts by weight of red ginseng to 350 to 450 parts by weight of purified water, extracting an effective ingredient by irradiating ultrasonic waves at 75 to 85 ° C for 20 to 30 hours , Lyophilized and pulverized, and stirred at a speed of 3000 to 5000 rpm for 4 to 6 hours using a wet microfine grinder.

According to still another more preferred characteristic of the present invention, the sea cucumber nano extract is prepared by immersing 100 parts by weight of sea cucumber in 600 to 700 parts by weight of purified water, extracting the active ingredient by irradiating ultrasound at 45 to 55 ° C for 65 to 75 hours, Lyophilized and pulverized, and stirred for 4 to 6 hours at a speed of 3000 to 5000 rpm using a wet ultrafine grinder.

According to a further preferred feature of the present invention, the mixture is further comprised of white rice extract, licorice extract, horsetail extract and green tea extract.

According to a further preferred feature of the present invention, 5 to 15 parts by weight of the white rice extract, 30 to 150 parts by weight of the licorice extract, 30 to 150 parts by weight of the marigold extract and 30 to 200 parts by weight of the green tea extract are added to 100 parts by weight of the mixture .

The object of the present invention can also be achieved by providing a cosmetic composition containing a nanoized natural component, which is produced by the method for producing a cosmetic composition containing the nanoized natural component.

According to a preferred feature of the present invention, the cosmetic composition containing the nanoized natural component is applied to one formulation selected from the group consisting of lotion, cream, toner, skin, essence, cleansing and shampoo.

The cosmetic composition prepared by the method of manufacturing the cosmetic composition containing the nanoized natural ingredients according to the present invention and the cosmetic composition prepared by the method of the present invention are excellent in moisturizing, whitening and antioxidative effects, And exhibits an excellent effect of providing a cosmetic composition containing a nanoized natural ingredient exhibiting a low effect of skin irritation.

1 is a flowchart showing a method of manufacturing a cosmetic composition containing a nanoized natural component according to an embodiment of the present invention.
FIG. 2 is a flowchart showing a method of manufacturing a cosmetic composition containing a nanoized natural component according to another embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention and physical properties of the respective components will be described in detail with reference to the accompanying drawings. However, the present invention is not limited thereto, And this does not mean that the technical idea and scope of the present invention are limited.

The method for producing a cosmetic composition containing a nanoized natural ingredient according to the present invention comprises a step (S101) of producing a culture liquid by inoculating Bacillus subtilis, lactic acid bacteria and yeast into a mixture containing a red ginseng extract and a sea cucumber nano extract (S101) A centrifuging step S103 for centrifuging the culture liquid prepared through the manufacturing step S101 and a stabilization step S105 for stabilizing the separated mixture through the centrifuging step S103.

In step (S101), the culture solution is prepared by inoculating Bacillus subtilis, lactic acid bacteria and yeast into a mixture containing the red ginseng nano extract and the sea cucumber nano extract. In 100 parts by weight of the mixture containing the red ginseng nano extract and the sea cucumber nano extract 0.05 to 1 part by weight of Bacillus subtilis, 0.05 to 1 part by weight of lactic acid bacterium and 0.05 to 1 part by weight of yeast are inoculated and cultured at a temperature of 20 to 30 DEG C for 20 to 30 hours.

The mixture is prepared by mixing 100 parts by weight of purified water, 1 to 5 parts by weight of red ginseng nano extract, and 1 to 5 parts by weight of sea cucumber nano extract. The red ginseng nano extract is prepared by adding 100 parts by weight of red ginseng to 350 to 450 parts by weight of purified water, To 85 ° C for 20 to 30 hours to extract an active ingredient, followed by lyophilization, pulverization, and agitation for 4 to 6 hours at a speed of 3000 to 5000 rpm using a wet ultrafilter, The sea cucumber nano extract is prepared by immersing 100 parts by weight of sea cucumber in 600 to 700 parts by weight of purified water and extracting an active ingredient by irradiating ultrasound at 45 to 55 ° C for 65 to 75 hours to lyophilize the powder, At a speed of 3000 to 5000 rpm for 4 to 6 hours.

When the content of the red ginseng nano extract and the sea cucumber nano extract is less than 1 part by weight, the effective amount of the red ginseng nano extract and the sea cucumber nano extract is high, When the content of the red ginseng nano extract or the sea cucumber nano extract is more than 5 parts by weight, the effect is not greatly improved but the manufacturing cost is increased.

The Bacillus subtilis is cultivated in a concentration of 10 ¹⁰ cfu / ml in a LB broth medium to be used as a culture seed bacterium. The lactic acid bacterium is inoculated in an amount of 0.05 to 1 part by weight, and lactobacillus acidophillus strains were cultured in MRS medium at a concentration of 5 × 10 ⁹ cfu / ml and used as culture seeds.

Saccharomyces cerevisiae was used as a yeast strain. The yeast was cultured in a Potato Dextros Broth medium at a concentration of 5 × 10 ⁹ cfu / ml and used as a fermentation starter.

After mixing the Bacillus subtilis, lactic acid bacteria and yeast into the mixture through inoculation of the strain, the air injection is stopped and the stirring speed is adjusted to about 10 rpm to cultivate the anaerobic bacterium. This process is performed by mixing the red ginseng nano extract and sea cucumber nano extract The active ingredient contained therein is biologically pulverized to reduce the molecular weight of the active ingredient and the useful ingredient that is not pulverized by aerobic bacteria can be lowered in molecular weight through biological pulverization using lactic acid bacteria and yeast under anaerobic conditions, And the metabolite produced in the fermentation process has an effect of softening and softening the skin.

The mixture may further contain a white rice extract, a licorice extract, a marigold extract and a green tea extract. More particularly, 100 parts by weight of the mixture may contain 5 to 15 parts by weight of the white rice extract, 30 to 150 parts by weight of the licorice extract, 30 to 150 parts by weight of the extract and 30 to 200 parts by weight of the green tea extract.

The white rice extract contains 5 to 15 parts by weight and is produced through the method described in Korean Patent Registration No. 10-1047588 and the like. Since it exhibits a very strong melanin production inhibitory effect, it exhibits skin whitening effect. The content of the white rice extract When the content of the white rice extract is more than 15 parts by weight, the effect is not greatly improved but the production cost is increased.

In addition, the licorice extract is contained in an amount of 30 to 150 parts by weight and is prepared by the method described in Korean Patent No. 10-1017922. It is preferable to use an extract obtained by raising the content of glabridin up to 35% by weight Do.

The glabridin contained in the licorice extract has excellent whitening activity because penetration into the skin is easier than other whitening substances. When the content of the licorice extract is less than 30 parts by weight, the above effect is insignificant If the content of the licorice extract is more than 150 parts by weight, the effect is not greatly improved but the manufacturing cost is increased.

In addition, the above-mentioned mashin extract contains 30 to 150 parts by weight of the extract, and is produced by the method described in Korean Patent Registration No. 10-0969269. Particularly, it has excellent antimicrobial activity and is effective against pathogens such as Staphylococcus aureus, Typhoid fungus, Escherichia coli, It exhibits excellent antibacterial activity against bacteria, is effective for soothing sensitive skin, produces skin keratin, inhibits clogging of hair follicles, promotes hair hair growth effect, and suppresses hair loss. When the content of the extract is less than 30 parts by weight, the above-mentioned effect is insignificant. If the content of the extract is more than 150 parts by weight, the effect is not greatly improved but the production cost is increased.

In addition, the green tea extract contains 30 to 200 parts by weight of the extract, which is prepared by the method described in Korean Patent Registration No. 10-0366949, and exhibits an effect of suppressing skin aging through excellent antioxidative action.

If the content of the green tea extract is less than 30 parts by weight, the effect is insignificant. If the content of the green tea extract is more than 200 parts by weight, the effect is not greatly improved but the manufacturing cost is increased.

The centrifuging step S103 is a step of centrifuging the culture solution prepared in the culturing solution preparation step S101. The culture solution prepared in the culturing solution preparation step S101 is put into a centrifuge, and centrifuged at 3000 to 8000 rpm Centrifuging at a speed of 5 to 10 minutes to remove foreign substances, Bacillus subtilis, lactic acid bacteria, yeast, etc. contained in the culture solution.

The stabilization step (S105) is a step of stabilizing the separated mixture through the centrifugation step (S103). The separated mixture through the centrifugation step (S103) is maintained at a temperature of 25 to 35 ° C for 20 to 30 hours Is stabilized.

After the stabilization step (S105), the separated mixture is stabilized through the centrifugation step (S103) to produce a cosmetic composition exhibiting uniform physical properties. The cosmetic composition may be in the form of a lotion, a cream, Toner, skin, essence, cleansing and shampoo.

Between the centrifugal separation step (S103) and the stabilization step (S105), an additive mixing step (S104) may be further performed in which the germanium aqueous solution and the sulfur aqueous solution are mixed into the culture solution separated through the centrifugal separation step (S103) , 0.01 to 0.5 parts by weight of a germanium aqueous solution and 0.5 to 10 parts by weight of an aqueous sulfuric solution are mixed with 100 parts by weight of the culture solution separated through the centrifugal separation step (S103) and mixed at a rate of 45 to 55 rpm for 25 to 35 minutes desirable.

The germanium aqueous solution is prepared by mixing 1 to 20 parts by weight of organic germanium with 100 parts by weight of purified water, introducing the mixture into an ultrasonic wave extractor, and reacting the mixture for 4 to 6 hours. 20 parts by weight of the germanium compound is added to an ultrasonic extractor and reacted for 4 to 6 hours. The biscarboxylic ethyl germanium sesquioxide component contained in the germanium aqueous solution has antioxidative effects showing acne relieving and therapeutic effects, radical scavenging ability, (JP63183570, JP62111908, US4229468, US4889715, US4623667, KASai Miracle cure, Organic Germanium, Japan Publications Inc, ISBN (Japan), and the like. 0-87040-1, 1980).

In addition, since the sulfuric aqueous solution exhibits effects such as inhibition of microbial production and exfoliation, the cosmetic composition containing the nanoized natural component produced by containing the germanium aqueous solution and the sulfur aqueous solution as described above has excellent antioxidative effect and exfoliation .

Hereinafter, the method for preparing a cosmetic composition containing a nano-sized natural component according to the present invention and the physical properties of the cosmetic composition prepared through the method will be described with reference to examples.

≪ Example 1 >

10 parts by weight of white rice extract, 90 parts by weight of licorice extract, 90 parts by weight of mash extract and 120 parts by weight of green tea extract were mixed with 100 parts by weight of a mixture (100 parts by weight of purified water, 3 parts by weight of red ginseng nano extract and 3 parts by weight of sea cucumber nano extract) 0.5 part by weight of Bacillus subtilis, 0.5 part by weight of lactic acid bacterium, and 0.5 part by weight of yeast were inoculated and cultured at 25 DEG C for 24 hours to prepare a culture solution. The culture solution was centrifuged and stabilized at 30 DEG C for 24 hours A cosmetic composition containing the nanoized natural ingredients was prepared.

≪ Example 2 >

0.25 parts by weight of germanium aqueous solution and 5 parts by weight of sulfuric aqueous solution were added to 100 parts by weight of the centrifuged culture solution to prepare a cosmetic composition containing nanoized natural ingredients.

The stability of the cosmetic composition containing the nanoized natural component prepared in Example 2 was evaluated according to the following Table 1.

(However, the stability is shown by the accelerated test for formulation stability at 4 ° C, 30 ° C and 45 ° C for 4 weeks.)

<Table 1>

As shown in Table 1 above, it can be seen that the cosmetic composition containing the nanoized natural component produced through Example 2 of the present invention exhibits excellent stability against temperature change.

Production Example 1 Production of Lotion

The cosmetic composition containing 5% by weight and 10% by weight of the cosmetic composition containing the nanoized natural component prepared in Example 2 was prepared and the ingredients thereof are shown in Table 2 below. The skin elasticity, skin- The wrinkle-improving effect is shown in Table 3 below.

(Except that the mixture was dispersed with a paddle mixer at 11 and heated to 80 DEG C, and then 2, 3, 4, and 10 were added in order and dissolved by stirring to dissolve the mixture. The mixture was stirred at 40 ° C for 1, 6, and 7 hours. After sufficient agitation, the mixture was aged at 37 ° C. Skin flexibility, skin moisturizing effect, moisturizing effect, Was used for 50 days for women in their 20s and 30s for a week and then the average was calculated by the 5 point scoring method of 1; very poor, 2; poor, 3; acceptable, 4; good, .

<Table 2>

<Table 3>

As shown in Table 3 above, it can be seen that the cosmetic composition containing the cosmetic composition containing the nanoized natural ingredients prepared in Example 2 of the present invention is excellent in skin flexibility, skin-spreading ability, moisturizing effect and wrinkle-reducing effect.

Preparation Example 2 Preparation of Essence (O / W Type)

An essence containing 5% by weight of the cosmetic composition containing the nanoized natural ingredient prepared in Example 2 was prepared and its ingredients are shown in Table 4 below.

(Except that No. 2 and No. 3 were heated to 80 ° C and dispersed with a paddle mixer, and then 4, 5, and 6 were added in order to dissolve and dissolve in 6, 7, 8, 9, 10, 11, 12, and 13 were melted by heating at 70 ° C and then stirred and added (paddle 15 rpm, Homo 2,000 rpm, 10 min) After sufficiently stirring, the mixture is stirred at 40 ° C for 16 times, aged sufficiently.

Unmodified: Prepare by the same process as above, but add only 14, 15 times in the additive part after emulsification.)

<Table 4>

PREPARATION EXAMPLE 3 Cream Production (O / W Type)

The cream containing 5% by weight of the cosmetic composition containing the nanoized natural ingredients prepared in Example 2 was prepared and the ingredients thereof are shown in Table 5 below.

2, 3 and 4 were dissolved in a paddle mixer and dispersed, and then 5, 6, 7, and 8 were added in order and dissolved by stirring. , 11, 12, 13, 14, 15, 16, 17, 18, and 19 were melted by heating at 70 ° C. and then stirred and stirred (paddle 15 rpm, Homo 2,000 rpm, 10 min) The mixture is stirred at 40 ° C for 20 times, agitated sufficiently and stirred.

Unsupplemented: Prepared by the same process as the above-mentioned preparation example, but 20 fragrant parts are added after emulsification.)

<Table 5>

The skin moisturizing power of the lotion, essence and cream prepared in Preparation Examples 1 to 3 was measured and shown in Table 6 below.

(However, as for the skin moisturizing ability, 22 women in their 20s to 50s who feel skin dryness were selected as subjects, and the above lotion, essence and cream were applied to the face twice in order For one month. The skin conductivity was measured at a constant temperature and humidity condition (temperature: 15 to 22 ° C, relative humidity: 40 to 45%) before starting the application using a moisture retention meter, and the skin conductivity was measured after 2 weeks and 4 weeks The rate of increase of moisturizing was evaluated. As a control, cosmetics containing no nanoized natural ingredients were selected, and a mixture of moisturizing ingredients (glycerin 1%, hydrolyzed collagen 1%, solvent: purified water) was used as a positive control, Values are expressed as average values.

The cosmetic lotion used was one containing 10% by weight of cosmetic composition containing nanoized natural ingredients.

<Table 6>

As shown in Table 6 above, no skin condition or specific skin irritation was found during the 4-week moisturizing test, and the lotion, essence, and cream containing the cosmetic composition prepared in Example 2 of the present invention were used , It was found that the skin conductivity was excellent and the moisturizing degree was improved.

Preparation Example 4 Preparation of Cleansing Cream

A cleansing cream containing 5% by weight of the cosmetic composition containing the nanoized natural ingredient prepared in Example 2 was prepared and its ingredients are shown in Table 7 below.

(However, in the 17th heated to 80 ° C, 2,3,4 were stirred and dissolved by a paddle mixer, and then 5, 8, 9, 10, 11, and 12 were sequentially added in a separate container, After 6 to 7 times of stirring, the mixture is stirred. After saponification, the mixture is cooled, and 1, 14 and 15 are added at 50 ° C. After sufficiently stirring, 16 times at 40 ° C is added. }

<Table 7>

It was confirmed that the cleansing cream composed of the ingredients shown in Table 7 on the above was soft and had a large amount of foam, and the makeup was well cleared.

&Lt; Preparation Example 5 > Production of shampoo

A shampoo containing 10% by weight of a cosmetic composition containing the nanoized natural ingredient prepared in Example 2 was prepared and the ingredients thereof are shown in Table 8 below. The moisturizing effect, the softening effect, the hair cracking prevention effect And dandruff removal effects were measured and shown in Table 9 below.

(Except that 2 or 3 times of heating at 80 ° C were dissolved and dispersed by paddle mixer, dissolving and dispersing 4 and 5 times to dissolve the paddle mixer at 12 rpm to obtain 6, 7, 8 and 9 times And 10, 11, and 12 are added thereto, and the mixture is sufficiently stirred. Subsequently, the mixture is stirred at 40 ° C for 13, 15 times, and aged. The moisturizing effect of the prepared shampoo, The effect of preventing hair cracking and the effect of removing dandruff were selected as the first group of 10 male and 12 female 20- to 50-year-old male and female dendritic and excessive sebum producing surveys, and hair was split and stiff Twelve men and fourteen women were selected as the second model (duplicate selection), and the shampoo prepared in Manufacturing Example 5 was used for one month twice a day, ; poor; 3; acceptable; 4; good; 5; very good. W is shown as an average value.)

<Table 8>

<Table 9>

As shown in Table 9 above, the shampoo prepared in Production Example 5 showed no symptoms of the scalp and specific skin irritation during the clinical experiment, and it was found that the moisturizing effect of the scalp and hair, the softness and cracking prevention effect of hair, Was evaluated as having excellent antimicrobial activity.

Especially, the recovery effect of hair damaged by use of a hair dryer and a pump was evaluated to be excellent.

In the experiment of the moisturizing effect of the scalp and hair of the present invention and the effect of preventing the hair from softening and cracking, the content of the cosmetic composition containing the nanoized natural ingredient was 10% by weight in the range in which the formulation could be maintained, It was evaluated that skin irritation was low due to no side effects such as skin seizures.

Accordingly, the cosmetic composition prepared by the method of manufacturing the cosmetic composition containing the nanoized natural component according to the present invention is excellent in moisturizing, whitening and antioxidative effect, Since the stimulation exhibits a low effect, it can be applied to formulations such as lotion, cream, toner, skin, essence, cleansing and shampoo.

S101; Culture medium preparation step
S103; Centrifugation step
S104; Additive mixing step
S105; Stabilization phase

Claims (10)

A step of preparing a culture solution by inoculating Bacillus subtilis, lactic acid bacteria and yeast into a mixture containing red ginseng nano extract and sea cucumber nano extract;
A centrifugal separation step of centrifuging the culture solution prepared through the culture solution production step; And
And a stabilization step of stabilizing the separated mixture through the centrifugal separation step,
The red ginseng nano extract is prepared by adding 100 parts by weight of red ginseng to 350 to 450 parts by weight of purified water and extracting the active ingredient by irradiating ultrasonic waves at a temperature of 75 to 85 ° C for 20 to 30 hours and then lyophilizing to powder, Using a pulverizer at a speed of 3000 to 5000 rpm for 4 to 6 hours,
The sea cucumber nano extract is prepared by immersing 100 parts by weight of sea cucumber in 600 to 700 parts by weight of purified water and extracting an active ingredient by irradiating ultrasound at 45 to 55 ° C for 65 to 75 hours to lyophilize the powder, At a speed of 3000 to 5000 rpm for 4 to 6 hours,
And an additive mixing step of mixing 0.01 to 0.5 parts by weight of germanium aqueous solution and 0.5 to 10 parts by weight of sulfur aqueous solution to 100 parts by weight of the culture liquid separated through the centrifugal separation step is further performed between the centrifugal separation step and the stabilization step Wherein the cosmetic composition contains a nano-sized natural component.
delete The method according to claim 1,
0.05 to 1 part by weight of Bacillus subtilis, 0.05 to 1 part by weight of lactic acid bacterium and 0.05 to 1 part by weight of yeast are inoculated in 100 parts by weight of a mixture containing red ginseng nano extract and sea cucumber nano extract, To 30 hours. The method for producing a cosmetic composition according to claim 1,
The method according to claim 1,
Wherein the mixture comprises 100 parts by weight of purified water, 1 to 5 parts by weight of red ginseng nano extract, and 1 to 5 parts by weight of sea cucumber nano extract.
delete delete The method according to claim 1,
Wherein the mixture further contains a white rice extract, a licorice extract, a marigold extract, and a green tea extract.
The method of claim 7,
Wherein the mixture further contains 5 to 15 parts by weight of a white rice extract, 30 to 150 parts by weight of a licorice extract, 30 to 150 parts by weight of a marigold extract and 30 to 200 parts by weight of a green tea extract in 100 parts by weight of the mixture. &Lt; / RTI &gt;
A cosmetic composition containing a nano-structured natural ingredient, characterized in that it is prepared by the method for producing a cosmetic composition containing a nano-natural ingredient according to any one of claims 1 to 7.
The method of claim 9,
Wherein the cosmetic composition containing the nanoized natural ingredient is applied to one formulation selected from the group consisting of lotion, cream, toner, skin, essence, cleansing and shampoo.

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