KR101570346B1 - The cosmetics and functional food composition containing N-feruloyltyramine improving anti-oxidant, anti-inflammatory and anti-wrinkling containing Lycium chinense MILL STEM extracts - Google Patents

Abstract

The present invention relates to a method for the isolation and purification of active ingredients from the extracts of P. japonicum tree having anti-inflammatory or anti-wrinkle activity, fractions thereof and the extracts of P. japonicus, and to provide cosmetics having antioxidant, anti- Or a health functional food composition.

Description

 FIELD OF THE INVENTION The present invention relates to cosmetics and functional food compositions containing antioxidant, anti-inflammatory and anti-wrinkle containing N-feruloyltyramine,

The present invention relates to a composition containing N-feruloyltyramine, and more particularly to a composition containing N-feruloyltyramine as an active ingredient, Antioxidant, anti-inflammatory and wrinkle-improving cosmetic compositions and health functional food compositions.

The cosmetics industry has been growing steadily, especially functional cosmetics. Among the above-mentioned functional cosmetics, the functional cosmetics market for skin aging improvement and wrinkle improvement is the field with the highest growth rate. Accordingly, materials for functional cosmetics for skin aging, antioxidation and wrinkle improvement are actively being developed.

Consumers' interest in safety has increased with the improvement of the economic level of the people and the change in the consumption style that pursues quality rather than quantity. Therefore, the demand for natural cosmetics using raw materials and minimizing the use of synthetic materials is rapidly increasing The development of functional cosmetic materials for skin aging, antioxidation, and wrinkle improvement is centered on natural materials rather than chemical ingredients. The image of functional cosmetics containing such natural materials as active ingredients is very eco-friendly to consumers .

However, it is difficult to ensure homogeneity of components and quality according to the cultivation and storage of the natural material, and as a result, the content of the active ingredient may not be uniform, and the active ingredient or pharmacologically active ingredient is unclear.

Therefore, what is most important in the development of a natural material for functional cosmetics is to identify a single compound whose effect is tested among various ingredients contained in a natural or natural extract, and to test the efficacy of the effective ingredient to determine a cosmetic composition Is urgently needed.

Gugija is a medicinal herb that is naturally grown or cultivated in Korea, China, Taiwan, Japan and Europe. Gugija uses fruits, leaves and roots in its parts. The gugija berries, leaves, roots, and the like have the same functions as betaine, rutin, kukoamine A and betasitosterol. A large amount of functional ingredient is contained. In addition, vitamin A, B1, B2, C calcium, phosphorus, iron, zinc, nicotinic acid (nicotinic acid) is rich in nutrients.

Korean Patent Laid-Open No. 10-2004-0095948 discloses a weight loss effect of hot water extracts from a branch leaves, and Korean Patent Laid-Open No. 10-2004-0095947 discloses a method of reducing weight loss Discloses a composition relating to an increase in the amount of the composition. However, the above references do not suggest or disclose a functional cosmetic composition for antioxidant, anti-inflammation or wrinkle reduction containing the extract of Guanxi seed tree or a specific single compound separated therefrom.

The cosmetics industry has been growing steadily, especially functional cosmetics. Among the above-mentioned functional cosmetics, the functional cosmetics market for skin aging improvement and wrinkle improvement is the field with the highest growth rate. Accordingly, materials for functional cosmetics for skin aging, antioxidation and wrinkle improvement are actively being developed.

Consumers' interest in safety has increased with the improvement of the economic level of the people and the change in the consumption style that pursues quality rather than quantity. Therefore, the demand for natural cosmetics using raw materials and minimizing the use of synthetic materials is rapidly increasing The development of functional cosmetic materials for skin aging, antioxidation, and wrinkle improvement is centered on natural materials rather than chemical ingredients. The image of functional cosmetics containing such natural materials as active ingredients is very eco-friendly to consumers .

However, it is difficult to ensure homogeneity of components and quality according to the cultivation and storage of the natural material, and as a result, the content of the active ingredient may not be uniform, and the active ingredient or pharmacologically active ingredient is unclear.

Therefore, what is most important in the development of a natural material for functional cosmetics is to identify a single compound whose effect is tested among various ingredients contained in a natural or natural extract, and to test the efficacy of the effective ingredient to determine a cosmetic composition Is urgently needed.

Gugija is a medicinal herb that is naturally grown or cultivated in Korea, China, Taiwan, Japan and Europe. Gugija uses fruits, leaves and roots in its parts. The gugija berries, leaves, roots, and the like have the same functions as betaine, rutin, kukoamine A and betasitosterol. A large amount of functional ingredient is contained. In addition, vitamin A, B1, B2, C calcium, phosphorus, iron, zinc, nicotinic acid (nicotinic acid) is rich in nutrients.

Korean Patent Laid-Open No. 10-2004-0095948 discloses a weight loss effect of hot water extracts from a branch leaves, and Korean Patent Laid-Open No. 10-2004-0095947 discloses a method of reducing weight loss Discloses a composition relating to an increase in the amount of the composition. However, the above references do not suggest or disclose a functional cosmetic composition for antioxidant, anti-inflammation or wrinkle reduction containing the extract of Guanxi seed tree or a specific single compound separated therefrom.

The above object of the present invention can be achieved by a method for producing a plant extract, comprising the steps of: obtaining an ethanol extract of Guanxi japonica tree and conducting hexane, chloroform, ethyl acetate, and butanol organic solvent fractions therefrom; Analyzing the components of the ethanol extract of the goat tree, hexane, chloroform, ethyl acetate and butanol fractions obtained in the above step, separating and purifying the active substance from the resultant; The antioxidative, anti-aging, anti-inflammatory and anti-wrinkle activities of the extracts of ethanol extract, chloroform fractions and active substances obtained from each of the above steps were measured.

The present invention provides an extract of Ganoderma lucidum having anti-inflammatory or anti-wrinkle activity and fractions thereof and a cosmetic or health functional food composition having antioxidant, anti-aging, anti-inflammatory or anti-wrinkle activity containing the extract as an active ingredient.

In addition, the active ingredients having anti-inflammatory or anti-wrinkle activity are isolated and purified from the extracts of P. japonicus, Antioxidant, anti-aging, anti-inflammatory or anti-wrinkle activity.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a diagram illustrating the entire process of identifying and isolating and purifying an organic compound 1 derived therefrom and a solvent fraction thereof of the present invention.
FIG. 2 is a graph showing the HPLC analysis of the ethanol extract of Gujuga gigas on the present invention.
FIG. 3 is a graph of HPLC analysis of the chloroform fractions of the present invention.
FIG. 4 is a graph of HPLC analysis of ethyl acetate fractions of the present invention.
Fig. 5 is a graph showing the results of HPLC analysis of the organic compound 1 derived from the Asiatic root of the present invention. Fig.
6 is a graph showing the ¹ H-NMR spectrum of the organic compound 1 derived from the Aspergillus oryzae according to the present invention.
7 is a graph showing the ¹³ C-NMR spectrum of the organic compound 1 derived from the Aspergillus oryzae according to the present invention.
FIG. 8 is a diagram showing the entire process of identification and identification of the organic compound 2 derived from the Aspergillus oryzae of the present invention.
9 is a graph showing the results of HPLC analysis of the organic compound 2 derived from the Aspergillus oryzae of the present invention.
10 is a graph showing the ¹ H-NMR spectrum of the organic compound 2 derived from the Aspergillus oryzae of the present invention.
11 is a graph showing the ¹³ C-NMR spectrum of the organic compound 2 derived from the Aspergillus oryzae according to the present invention.
FIG. 12 is a graph showing the results of measurement of DCFH-DA of the extract of Aspergillus oryzae according to the present invention.
FIG. 13 is a graph showing the anti-inflammatory effect of the extract of Aspergillus oryzae in the present invention.
FIG. 14 is a graph showing the anti-inflammatory effect of the chloroform fractions of the present invention.
15 is a graph showing the anti-inflammatory effect of the organic compound 1 derived from the Aspergillus oryzae of the present invention.
FIG. 16 is a graph showing the results of measuring the effect of increasing the collagen synthesis of the extract of Aspergillus oryzae according to the present invention.
FIG. 17 is a graph showing the results of measurement of the effect of increasing the collagen synthesis of the fractions of the present invention.
Fig. 18 is a graph showing the result of measuring the effect of increasing the collagen synthesis of the organic compound 1 derived from the Asiatic root of the present invention.

According to the present invention, the extract of Guanxi japonica tree can be extracted with water, ethanol, methanol, butylene glycol, propanediol or a mixed solvent thereof, preferably extracted with 60% ethanol. In addition, the above-mentioned extract may be a dried product obtained by drying an extract obtained by extraction, a diluted solution or concentrate of the extract, a fraction of the extracted solution, an extract, or a preparation or purified product of the extract.

In addition, in the present invention, the time and temperature for extracting the goji chestnut are not particularly limited, but are preferably extracted three times at room temperature for 4 hours each time.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a flexible lotion, a convergent lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of spraying, chlorofluorohydrocarbons, propane / Or a propellant such as dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component.

When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as polyoxyethylene sorbitol ester, microcrystalline cellulose or the like may be used as a carrier component.

When the formulation of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate and the like may be used as a carrier component.

The cosmetic composition of the present invention can be applied to face or the like once or several times per day.

The present invention provides a health functional food composition comprising as an active ingredient an extract of Aspergillus oryzae prepared according to the present invention, a fraction thereof or an organic compound derived from Aspergillus oryzae. The extract, the fraction thereof or the organic compound derived from Trichoderma sp. Are preferably contained in an amount of 0.1 to 99% by weight based on the total weight of the health functional food composition.

The food composition of the present invention can be used as a health functional food. The term "functional food" as used herein means a food prepared and processed using a raw material or ingredient having a useful function in the human body pursuant to Law No. 6727 on Health Functional Foods, and the term "functional" And function of the nutrient for the purpose of obtaining a beneficial effect in health use such as controlling the nutrient or physiological action.

The food composition of the present invention may contain conventional food additives, and the suitability of the above-mentioned "food additives" is not limited by the general rules and general test methods approved by the Food and Drug Administration It shall be determined according to standards and standards for the item.

Examples of the substances found in the above-mentioned "food additives" include natural compounds such as ketones, chemical compounds such as glycine, potassium citrate, nicotinic acid and cinnamic acid, coloring pigments, licorice extracts, crystalline cellulose, high- L-glutamic acid sodium preparations, noodle-added alkalis, preservative preparations, tar pigment preparations and the like.

The hard capsule of the capsule type health functional food can be prepared by filling a normal hard capsule with a mixture of herbal medicine extract with an additive such as an excipient or a granular product thereof or a granulated product with a hard gelatinized product. Of the present invention may be filled in a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a coloring agent, a preservative and the like, if necessary.

In case of a ring type health functional food, a mixture of excipient, binder, disintegrant, etc. may be formulated into a herbal medicine extract by an appropriate method. If necessary, the preparation may be mixed with white sugar or other suitable skin care agent or a starch, talc or a suitable substance It is possible to make a ring.

In the granular health functional food, a mixture of an excipient, a binder and a disintegrant may be prepared into a granular form by an appropriate method, and if necessary, a flavoring agent, a mating agent and the like may be contained in the herbal medicine extract. The definitions of the above-mentioned excipients, binders, disintegrants, lubricants, mating agents, flavoring agents and the like of the present invention are described in documents known in the art and include the same or similar functions (see, for example, Korean Association of Pharmacy, 5th ed., Pp. 33-48, 1989).

Hereinafter, the present invention will be described in more detail with reference to the following examples and experimental examples. However, the following examples and experimental examples are provided only for illustrating the present invention, and thus the scope of the present invention is not limited thereto.

<Example 1> The present invention Preparation and Isolation and Identification of anti-inflammatory or Hangzhou name of the active material therefrom Gugija biloba

In order to isolate and identify single compounds with anti - inflammatory or anti - wrinkle effect from the extracts of Gugija, the dried Gugai trunks were collected and used in the experiment.

Manufacturing example  1: Preparation of the extract of Gujuzuga japonica tree according to the present invention

1 kg of the dried Gugija trunk was pulverized and pulverized. 1 kg of the Gugija trunk powder was extracted with 10 times of 60% ethanol at room temperature for 3 days, and then filtered. The filtrate was concentrated under reduced pressure to prepare the Gugai trunk extract of the present invention And then used as a test material for the anti-inflammatory and anti-wrinkle activity test and the identification of active ingredients in the extract of Aspergillus oryzae.

Manufacturing example  2: Invention of the present invention Fraction

As shown in FIG. 1, the extract of the present invention prepared according to Preparation Example 1 was subjected to sequential solvent fractionation in the order of hexane, chloroform, ethyl acetate, and butanol To obtain respective fractions. Among these fractions, chloroform fractions were used as anti-inflammatory and anti-wrinkle activity test agents of the present invention and as a disclosing material for isolating active ingredients.

Experimental Example  1: The present invention relates to the extract of Gujuzan Fraction HPLC  analysis

The components of the ethanol extract, chloroform fraction and ethyl acetate fraction of the present invention obtained according to Preparation Examples 1 to 2 were analyzed by HPLC (Waters 2695).

The analytical columns were ODS columns (Kromasil 4.5 X 150 mm, 5 μm) and purified water and methanol were analyzed gradually for 50 min analysis time from 100: 0 to 0: 100 (v / v) mixing ratio. The extracts and fractions were injected at a flow rate of 1 mL / min, a column temperature of 40, and a sample injection volume of 10 ug / mL, respectively, and the detection wavelength was 318 nm. Analytical HPLC column chromatograms are shown in Figures 2 to 5.

Experimental Results As shown in FIG. 2, the main component of the Gugija edulis extract of the present invention, which was detected at 25 minutes, was changed to the chloroform fraction fractioned according to Preparation Example 2 as shown in FIG. 3, and the other components were dissolved in ethyl acetate It was confirmed that it was transferred into fractions.

Experimental Example  2: Isolation and Identification of Organic Compound 1 of the Present Invention

According to the analysis result of Experimental Example 1, 190 mg of the chloroform fraction fractioned according to Preparation Example 2 was isolated from the crude extract of Gujuza japonica extract as shown in FIG. 1, and the fixed phase was purified by silica gel column chromatography (v / v) 9: 1, 7: 1, and 6: 1, respectively, and the mobile phases were mixed with each other at a mixing ratio of As a solvent, and then fractionated by elution with polarity.

Each of the fractions obtained through the above silica gel column chromatography was applied to a TLC plate and developed, and fraction 7 having a high purity was obtained. This fraction was called organic compound 1. The organic compound 1 of the fraction obtained above was identified by HPLC, ¹ H-NMR and ¹³ C-NMR analysis.

400 was dissolved in the present invention the organic compound 1 was obtained in the NMR solvent CD ₃ OD for the HPLC analysis conditions were the same as in the analysis of the experimental example 1 except the sample conditions were ¹ H-NMR and ¹³ C-NMR analysis The ¹ H-NMR and ¹³ C-NMR spectra were obtained using a mHz NMR instrument and the spectra thereof were summarized as shown in Table 1 below.

As shown in FIG. 5, the organic compound 1 of the present invention was highly purified and the NMR spectra of FIGS. 6 to 7 and the results of ¹ H NMR and ¹³ C NMR, as shown in Table 1, The structure was determined as shown in the following formula (1). The organic compound 1 was found to be a phenylpropanoid amide N-feruloyltyramine represented by the following chemical formula 1.

&Lt; Formula 1 >

Experimental Example  3: Isolation and Identification of Organic Compound 2 of the Present Invention

According to the results of the above Experimental Example 1, the ethyl acetate fraction obtained according to Preparation Example 2 was separated by silica gel column chromatography (Silica gel, Merck, Silica gel 60) to isolate the main component from the ethyl acetate fraction of the present invention. , 70 to 230 mesh ASTM, Lot No. 1.07734.1000), and the mobile phase was eluted with a polar solvent by using chloroform: methanol as a solvent mixed at a mixing ratio of 85:15 (v / v) The pure organic compound 2 was purified.

The organic compound 2 of the present invention obtained above was identified by HPLC, ¹ H-NMR and ¹³ C-NMR analysis.

400 to dissolve the present invention the organic compound 2 obtained in the NMR solvent CD ₃ OD for the HPLC analysis conditions were the same as in the analysis of the experimental example 1 except the sample conditions were ¹ H-NMR and ¹³ C-NMR analysis The ¹ H-NMR and ¹³ C-NMR spectra were obtained using a mHz NMR instrument and the spectra were summarized as shown in Table 2 below.

As a result, as shown in FIG. 9, the organic compound 2 of the present invention was highly purified and the spectrum similar to that of the organic compound 1 was observed in the NMR spectrum of FIG. 10 to FIG. 11 and in the organic compound 2 No methoxy proton of δ 3.86 in organic compound 1 was observed.

Accordingly, the organic compound 2 of the present invention was found to be trans-N-caffeoyltyramine, which is a form in which the methoxy group is substituted with a hydroxy (-OH) group in the same skeleton as the organic compound 1, and its chemical structure is shown in the following Chemical Formula 2.

(2)

< Example  2> Functional Evaluation of Organic Compound 1 and Organic Compound 2 of the Invention

EXPERIMENTAL EXAMPLE 4: Preparation of DCFH -DA (2`, 7`- dichloro Antioxidant activity assay using fluorescin-diacetate

Energy production in cells involves the production of reactive oxygen species (ROS), a byproduct of mitochondrial respiration, which causes damage to proteins, fats and nucleic acids.

In order to protect the cell itself from this damage, the defense system works in the cell. However, if the balance of the free radical generation and the antioxidant system is broken, oxidative stress acts on the cell, which is a major cause of various diseases and cancers of the body including aging. do.

Therefore, the antioxidant ability of the extract is evaluated by measuring the amount of active oxygen formed in the cells for developing an antioxidant using a skin-friendly and stable plant as a raw material. DCFH-DA is a nonpolar molecule that can easily enter the cell and is separated by the intracellular esterase into DCFH, which is a nonpolar polar molecule. The DCFH-DA is a typical evaluation method using a fluorimetric assay using 2 ', 7`-dichlorofluorescin-diacetate. After oxidation by reactive oxygen species in the cells, they are converted to fluorescent DCF (2`, 7`-dichlorofluorescin), which is measured by a fluorometer.

The dermal fibroblasts (HDFn) were suspended in DMEM (containing 10% serum, 1% antibiotic), cultured at 37 ° C in a 5% CO ₂ incubator, and cells growing in the larval stage were mainly used for the experiment. Wells were inoculated in 96 wells at a rate of about 1 x 10 ⁵ cells per well. Then, the ethanol extract of Gujuza japonica obtained according to Preparation Example 1 was treated for each concentration and then cultured in a 5% CO ₂ incubator at 37 ° C for 20 minutes . Then, an oxidative stress-inducing substance, H ₂ O ₂ And 20 M of DCFH-DA (stock 20 mM) were added, and then the result was measured by spectrofluorophotometer under the conditions of excitation 485 nm and emission 535 nm, followed by measurement at 37 ° C. for 90 minutes. As a control group, only the formation of reactive oxygen species was measured without adding a sample, and compared with the group treated with the extract of the present invention for extracting active oxygen species, the inhibition rate of reactive oxygen species was calculated by the following equation 1, and all the experiments were repeated three times.

Equation 1

As shown in FIG. 12, when the cells were treated with ethanol extracts of 10, 50 and 100 ug / mL, the concentration-dependent activity was exhibited in the cells treated with 100 ug / mL of ethanol extract And the effect of reducing the concentration of active oxygen by 36.23% was confirmed.

Experimental Example 5: Anti- inflammatory activity test of Gugija tree ethanol extract, chloroform fraction and organic compound 1 of the present invention

Various inflammatory mediators are involved in the pathogenesis of inflammation, and the causes of the disease vary. Treatment of macrophages with LPS (lipopolysachride), known as endotoxin, increases NO biosynthesis by inducible NOS (iNOS) expression and induces an inflammatory response. NO is a pathological secondary signaling substance secreted by many cells, regulates the activity of COX and acts synergistically to the inflammatory response. By measuring NO produced by LPS induced by NOS in Raw 264.7 cells, the inhibitory effect of the extract on inflammation can be determined.

Mouse macrophage cell RAW 264.7 cells were treated with LPS, an inflammation inducing substance, to evaluate whether they inhibited inflammation. The cells were cultured in 96-well plates at a density of 2 × 10 ⁵ cells per well, cultured for 24 hours at 37 ° C. in a 5% CO ₂ incubator, replaced with fresh medium, treated with 1 μg LPS / The ethanol extract of Guanyi tree, the chloroform fractions obtained in accordance with Examples 1 and 2, and the organic compound 1 of the present invention were each administered at a concentration ranging from 1 ug / mL to 100 ug / mL for 24 hours. After incubation, the supernatant was taken and centrifuged at 13,000 rpm for 3 minutes to collect supernatant, reacted with Griess reagent reagent 1: 1, and absorbance was measured at 570 nm.

Experimental Results As shown in FIG. 13, when the RAW 264.7 cells were treated with 10, 50 and 100 μg / mL of ethanol extracts of the present invention, the anti-inflammatory activity of 5, 20 and 40% No cytotoxicity was noted at all of the concentrations.

As shown in FIG. 14, the fractions of the present invention showed excellent anti-inflammatory activity of 20, 82.5 and 98% at 10, 50 and 100 ug / mL, respectively. Therefore, the anti - inflammatory activity of the fractions fractionated with chloroform from the ethanol extract of P. japonicus was remarkably increased.

As shown in FIG. 15, the anti-inflammatory activity of the organic compound 1 of the present invention isolated from the chloroform fraction of the present invention was found to be 20, 70 and 85% at the concentrations of 10, 50 and 100 ug / mL, No cytotoxicity was noted at all of the concentrations.

Experimental Example 6: Enhancement of Collagen Synthesis Effect of Ethanol Extract, Chloroform Fraction and Organic Compound 1 of the present invention

Dermal fibroblasts (HDFn) were inoculated into 48 well plates at a concentration of 5 × 10 ⁴ cells / well in 0.3 mL of culture medium and cultured at 37 ° C. and 5% CO ₂ . After 24 hours, the extracts were cultured for 24 hours in a fresh medium containing the concentrations of the extracts. After culturing for 24 hours, the culture broth was collected and the amount of C-terminus of the collagen precursor was measured using an enzymes-linked immunoassay kit (Takara). In addition, a standard curve between collagen concentration of 0 ng and 640 ng was obtained using collagen solution 640 ng / mL, and the amount of collagen in the medium was calculated.

Experimental Results As shown in FIG. 16, it was confirmed that collagen production was increased by 5, 22 and 40% by 10, 50 and 100 ug / mL, respectively, No cytotoxicity was seen at all concentrations.

As shown in FIG. 17, it was also confirmed that collagen production was increased by 2, 15 and 23% at 10, 50 and 100 ug / mL, respectively, and no cytotoxicity was observed at all the concentrations . However, the effect of increasing the collagen biosynthesis of the fractions fractionated with chloroform from the ethanol extract of P. japonica did not increase.

As shown in FIG. 18, the collagen biosynthesis enhancing effect of the organic compound 1 of the present invention isolated from the chloroform fraction of the present invention was enhanced by 20, 30 and 40% at 10, 50 and 100 ug / mL, , And no cytotoxicity was observed at all the concentrations.

INDUSTRIAL APPLICABILITY As described above, the present invention relates to a method for the isolation and purification of active ingredients from the extracts of the Aspergillus oryzae having anti-inflammatory or anti-wrinkle activity, fractions thereof and extracts of Aspergillus oryzae, and their antioxidant, anti-aging, anti- , It is a very useful invention in the cosmetics or food materials industry.

Claims (4)

A functional cosmetic composition for anti-inflammation and skin wrinkle-improving, which comprises, as an active ingredient, a chloroform fraction obtained by sequential solvent fractionation of an ethanol extract of Tricholoma japonica tree stem powder with hexane, chloroform, ethyl acetate and butanol.
Wherein the chloroform fraction obtained by successively solvent fractioning the ethanol extract of the tree trunks of Gujuza japonica with hexane, chloroform, ethyl acetate and butanol is used as an active ingredient.
The anti-inflammatory functional food composition according to claim 2, wherein the composition has one of the formulations selected from the group consisting of capsules, pills, and granules.
3. The anti-inflammatory functional food composition according to claim 2, wherein the composition further comprises a pharmaceutically acceptable carrier, excipient or diluent.

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