KR100491362B1 - 면역 활성 증진 복합 다당체의 제조 방법 - Google Patents
면역 활성 증진 복합 다당체의 제조 방법 Download PDFInfo
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- KR100491362B1 KR100491362B1 KR10-2003-0004171A KR20030004171A KR100491362B1 KR 100491362 B1 KR100491362 B1 KR 100491362B1 KR 20030004171 A KR20030004171 A KR 20030004171A KR 100491362 B1 KR100491362 B1 KR 100491362B1
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- culture
- hemicellulose
- mushroom
- mycelium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8998—Hordeum (barley)
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/11—Preparation or pretreatment of starting material involving culturing conditions, e.g. cultivation in the dark or under defined water stress
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- General Chemical & Material Sciences (AREA)
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- Alternative & Traditional Medicine (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (7)
- 식물 조직체를 열수나 수산화 나트륨으로 추출하여 수득한 추출물을 헤미셀룰라아제는 포함되지 않는 효소액에 처리하는 단계와; 상기 효소액으로 처리된 추출물에 전배양된 담자균류의 균사액을 접종하고, 담자균류의 균사체가 헤미셀룰라아제를 분비하는 시기에 배양을 중단하여, 헤미셀룰로오스를 에너지원으로 사용하지 못하게 배양하는 단계; 및 상기 배양 후, 배양물로부터 헤미셀룰로오스를 분리, 정제하는 단계를 포함하는 것을 특징으로 하는 면역 활성 증진 복합 다당체의 제조방법.
- 제 1항에 있어서, 효소는 아밀라아제, 프로테아제, 셀룰라아제 및 이들의 혼합물로 이루어진 효소군으로 부터 선택되는 것을 특징으로 하는 면역 활성 증진 복합 다당체의 제조방법.
- 제 1항에 있어서, 담자균류는 표고버섯, 영지버섯, 차가버섯, 아가리쿠스버섯, 상황버섯, 운지버섯, 복령 중의 한 종을 선택하여 배양하는 것을 특징으로 하는 면역 활성 증진 복합 다당체의 제조방법.
- 제 1항에 있어서, 식물 조직체는 쌀, 보리, 밀, 옥수수, 수수, 귀리, 팥, 조, 기장으로부터 선택되는 것을 특징으로 하는 면역 활성 증진 복합 다당체의 제조방법.
- 제 1항에 있어서, 배양기간은 균체의 생장이 정상기에 있고, 배지 내 글루코오스의 농도는 초기농도와 비교하여 3∼59%인 조건에서 배양을 정지시키는 것을 특징으로 하는 면역 활성 증진 복합 다당체의 제조방법.
- 제 1항에 있어서, 헤미셀룰로오스의 분리, 정제는 상기 배양물을 마쇄하여 열수 추출하고, 열수 추출액에서 원심분리하여 상등액을 수집하고, 에탄올을 처리하여 침전물을 얻고, 침전물은 증류수에 녹이고 에탄올로 처리한 후 원심분리하여 다시 침전물을 얻고, 이를 증류수에 녹이고 분무 건조기에서 건조시켜 행하는 것을 특징으로 하는 면역 활성 증진 복합 다당체의 제조방법.
- 제 1항 내지 제 6항중 어느 한 항에 의해서 제조된 아라비녹실란이 포함된 헤미셀룰로오스로 구성된 면역 활성 증진 복합 다당체.
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KR10-2003-0004171A KR100491362B1 (ko) | 2003-01-22 | 2003-01-22 | 면역 활성 증진 복합 다당체의 제조 방법 |
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KR10-2003-0004171A KR100491362B1 (ko) | 2003-01-22 | 2003-01-22 | 면역 활성 증진 복합 다당체의 제조 방법 |
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KR20040067175A KR20040067175A (ko) | 2004-07-30 |
KR100491362B1 true KR100491362B1 (ko) | 2005-05-24 |
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WO2010082798A2 (ko) | 2009-01-19 | 2010-07-22 | 주식회사 알앤엘바이오 | 차가버섯 추출물, 영지버섯 추출물 및 상황버섯 추출물을 함유하는 조혈모세포 증식 촉진용 조성물 |
KR20160065256A (ko) | 2014-11-28 | 2016-06-09 | 사조동아원 주식회사 | 수용성 아라비노자일란 및 식이섬유 함량이 높은 밀기울의 가공방법 |
KR20200050483A (ko) | 2018-11-01 | 2020-05-12 | 사조동아원 주식회사 | 항산화 물질이 함유된 밀 배아로 부터 항산화 물질을 활성화하기 위한 효소처리 방법 |
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WO2010082798A2 (ko) | 2009-01-19 | 2010-07-22 | 주식회사 알앤엘바이오 | 차가버섯 추출물, 영지버섯 추출물 및 상황버섯 추출물을 함유하는 조혈모세포 증식 촉진용 조성물 |
KR20160065256A (ko) | 2014-11-28 | 2016-06-09 | 사조동아원 주식회사 | 수용성 아라비노자일란 및 식이섬유 함량이 높은 밀기울의 가공방법 |
KR20200050483A (ko) | 2018-11-01 | 2020-05-12 | 사조동아원 주식회사 | 항산화 물질이 함유된 밀 배아로 부터 항산화 물질을 활성화하기 위한 효소처리 방법 |
KR20210051240A (ko) | 2019-10-30 | 2021-05-10 | 사조동아원 주식회사 | 밀 배아로 부터 수용성 항염활성 물질을 활성화방법 |
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