JPH0283031A - Preparation of microcapsule - Google Patents

Abstract

PURPOSE:To provide a microcapsule high in quality and practicality by dispersing a water soluble or difficulty soluble amino acid and peptide into fats and oils and adding and dispersing the resulting slurry into a specified alkylene glycol. CONSTITUTION:To coat with fats and oils the substances to be encapsulated, i.e., amino acid, dipeptide and tripeptide, such substances are dispersed into the fats and oils and the resulting dispersed slurry is added and dispersed into the alkylene glycol having the molecular weight of 60-400 such as propylene glycol and ethylene glycol and, under rapid cooling, the fats and oils having a strong affinity for amino acid forms in the alkylene glycol the coating excellent in coatability over the surface of the substances to be encapsulated. Therefore, there are little or no dissolution of the coating material and exudation of the encapsulated substances, offering a microcapsule high in quality and practicality.

Description

[Detailed description of the invention] Qizumi Jōza ■ 几 Bunri The present invention has almost no bitterness derived from amino acids.

Amino acids that can be suitably used as foods and medicines.

The present invention relates to a method for producing microcapsules in which dipeptides, tripeptides, or salts thereof are used as a core material and coated with oil or fat.

Various methods have been known to obtain microcapsules by coating amino acids, etc. with fats and oils (Japanese Patent Publication No. 39-5911, No. 49-10).
No. 912, No. 49-45224).

However, the method described in Japanese Patent Publication No. 39-5911 is economically disadvantageous because it uses expensive silicone oil, fluorinated oil, etc. as the encapsulation medium. The problem is that it dissolves considerably.

Furthermore, Japanese Patent Publication No. 10912/1983 proposes a method for encapsulating water-soluble amino acids.

In this method, a poorly soluble amino acid or a mixture of a water-soluble amino acid and a poorly soluble amino acid is used as the core material.

There is a drawback that the coating with the membrane material is not sufficient and it is difficult to encapsulate it.

Furthermore, the method for manufacturing amino acid-containing oil and fat capsules described in Japanese Patent Publication No. 49-45224 uses water as the encapsulation medium and encapsulates at a temperature of 20 to 40°C, so the water temperature exceeds 40°C. The elution of the core substance amino acid into the medium (water) becomes large, and the melting point is 40%.
It is not possible to use only fats and oils with a temperature of ℃ or higher as a membrane material.

As described above, it has been difficult to obtain high-quality and practical amino acid-containing fat and oil microcapsules using conventional production methods.

The present invention was developed in view of the above circumstances, and allows amino acids and peptides to be microencapsulated, regardless of whether they are water-soluble or poorly soluble, with almost no dissolution of oil or fat in the membrane material or elution of core substances, and high quality. An object of the present invention is to provide a method for producing microcapsules that can produce highly practical amino acid and/or peptide-containing oil and fat microcapsules.

In order to achieve the above object, the present inventor has made extensive studies and found that one or more core substances selected from amino acids, dipeptides, tripeptides, and salts thereof are coated with oil or fat. In this case, an alkylene glycol having a molecular weight of 60 to 400, which hardly dissolves the core substance and fats and oils, is used as the encapsulation medium, and the core substance is preferably
After being dispersed in fats and oils heated to 0 to 100°C, this core substance dispersion slurry is preferably added and dispersed in an alkylene glycol having the above molecular weight heated to 40 to 100°C, and the slurry is then rapidly cooled. In alkylene glycol, fats and oils that have a high affinity with amino acids form a film with excellent coating properties on the surface of the core substance, and therefore, the film material (fats and oils)
We discovered that it is possible to obtain microcapsules in which the core substance is coated with oil and fat, which is of high quality and highly practical, with almost no dissolution of the core substance or elution of the core substance (amino acids, dipeptides, tripeptides, or their salts). He came up with an invention.

Therefore, in the present invention, after dispersing one or more core substances selected from amino acids, dipeptides, tripeptides, and salts thereof in fats and oils, the core substance dispersion slurry is mixed with an alkylene glycol having a molecular JL of 60 to 400. The present invention provides a method for producing microcapsules, which comprises adding and dispersing the above-mentioned core substance in a liquid to obtain microcapsules in which the core substance is coated with oil or fat.

The present invention will be explained in more detail below.

The oil and fat used as the membrane material in the method for producing microcapsules of the present invention is not particularly limited, but has a melting point of 100
℃ or lower, especially 40 to 80℃ or lower, is stable against heat, does not denature amino acids, and can be easily digested by human or other animal digestive enzymes, such as hydrogenated fish oil and hydrogenated whale oil. , animal oils such as hydrogenated beef tallow, hydrogenated castor oil, and hydrogenated soybean oil.

Vegetable oils such as hardened palm oil and palm stearin can be suitably used. Note that these oils and fats may be used alone or in combination, and it is also possible to use a mixture of hardened oil and unhardened oil.

In addition, as the core substance, one or more types selected from amino acids, dipeptides, tripeptides, and salts thereof are used. In this case, various amino acids and peptides are used depending on the purpose of use of the microcapsules. be able to. Specifically, the core substances include L-valine, L-leucine, L-isoleucine, L-threonine, L-phenylalanine, L-tryptophan, L-lysine, and DL-
Essential amino acids such as methionine and L-histidine, and L-
Glutamine, L-glutamic acid, L-asparagine, L
-Amino acids such as L-type amino acids such as aspartic acid and these D-type or dQ-type amino acids, glycylglycine (
Gly-Gly), leucylglycine (Leu G
ly), glycylhistidine (Gly-His),
Glycyrrhizine (Gly Lys) 1 Alanylglycine (Ala-aly), Glycylaspartic acid (
Gly Asp) I Serylglycine (Set-Gl
y), glycylleucylalanine (Gly-Leu
-Ala), glycylysylglycine (Gly-
Examples include di- or tripeptides such as Lys-Gly), and alkali metal salts or alkaline earth metal salts of these compounds. In addition, these compounds can be used individually or in mixture of 2 or more types.

Furthermore, the amino acids and peptides used as the core material preferably have a size of from a few minutes to several 100/a, especially 50 or less, from the viewpoint of ease of inclusion in microcapsules and ease of handling, when one particle is large. is preferably used after being finely pulverized.

Note that there is no problem in adding desired amounts of antioxidants such as tocopherol, pigments, fragrances, etc. as additives together with oils, fats, amino acids, and peptides into the membrane material and core material.

Therefore, the method for producing microcapsules of the present invention involves dispersing the above-mentioned core substance in oil and fat, and then adding and dispersing this dispersion slurry in alkylene glycol having a molecular weight of 60 to 400. As an encapsulation medium, it does not dissolve or denature oils, fats, or amino acids.
By using an alkylene glycol with a molecular weight of 60 to 400 that is stable against heat and has a viscosity of about 300 to 100 centipoise when heated, oils and fats that have a high affinity with amino acids in this alkylene glycol are used as the core substance. It forms a film with good coverage on the surface.

In this case, the alkylene glycol having a molecular weight of 60 to 400 is 1, for example, ethylene glycol.

1. Glycols such as 2-propanediol, 1,3-propanediol, 1,4-butanediol, diethylene glycol, triethylene glycol, tetraethylene glycol, polyethylene glycol #400. Dipropylene glycol #400, tripropylene glycol #400. Examples include polyalkylene glycols such as tetrapropylene glycol #400, particularly propylene glycol, ethylene glycol, di- and tri-glycols.

Tetraethylene glycol, di-, tri-, and tetrapropylene glycol can be suitably used.

Here, when dispersing the core substance in oil or fat, add 40% of the oil or fat.
The above-mentioned core substance is heated to ~100°C, particularly 50 to 90°C, and the content of the core substance in the heated fat is 1 to 80% (wt%).
, hereinafter the same), especially added to 30 to 70%,
It is preferable to disperse by stirring.

Next, the obtained core material dispersion slurry is added and dispersed in alkylene glycol having the above-mentioned specific molecular weight to perform encapsulation. The ratio by weight is 1/2 to 1/30. Especially 115~1/1
0 to 40 to 100℃, especially 50 to 9
After heating to 0"C, add and disperse the dispersion slurry,
The particle size of the microcapsules is from several parts to several parts, especially from 200 to 8
It is preferable to adjust to oOp.

In addition, when performing encapsulation by dispersing the core material dispersion slurry with alkylene glycol, first add a small amount of
Preferably, alkylene glycol is added to the core material dispersion slurry in an amount such that the ratio of the dispersion slurry to fullkylene gelcol is 1:0.25 to 1:5 by weight.
After performing stage encapsulation, a method may be suitably employed in which the first stage capsules are added to the remaining alkylene glycol to perform second stage encapsulation. In this case, the temperature of the first stage encapsulation is 40-100 °C, especially 50-90 °C, and the temperature of the second stage encapsulation is 40-100 °C, especially 50-90 °C.
The temperature is preferably 0°C.

Subsequently, when the liquid temperature of this alkylene glycol is rapidly lowered, preferably to 40° C. or lower, the oil or fat that is the membrane material solidifies, and encapsulation is completed.

The amino acid and/or peptide-containing oil microcapsules obtained in this way can be prepared by separating the microcapsules from alkylene glycol and redispersing them in a solvent such as ethanol, depending on the purpose of use. It can be used as a liquid, or as a powder by coating the surface of the microcapsule with a fine powder of corn starch, potato starch, etc., as various foods, medicines, etc.

According to the production method of the present invention, a high-quality and practical amino acid and/or peptide-containing oil microorganism is produced that hardly dissolves the membrane material (oil or fat) or elutes the core substance (amino acid and/or peptide). Capsules can be produced economically and easily. Furthermore, in the present invention, it is possible to encapsulate water-soluble and poorly soluble amino acids and peptides, and the bitter taste derived from amino acids can be hidden in the capsules, so the resulting microcapsules can be used as health foods, medical foods, etc. For example, it can be used in a variety of functional raw foods, including essential amino acids as a core substance, and highly nutritious liquid food for people who cannot consume their own meals.

EXAMPLE 11 - Skin Squeezing The present invention will be specifically explained below with reference to Examples and Comparative Examples, but the present invention is not limited to the Examples below.

[Example 1] 40 g of hardened beef tallow and 60 g of the amino acids shown in Table 1 were
Place in a 0aQ stirring tank and heat at 70°C.

After mixing, 50 g of propylene gelcol was added thereto to perform first-stage encapsulation.

Next, propylene gelcol 1Q was placed in the stirring tank of 3Q, heated to 90°C, and while stirring, the first stage capsules obtained above were added and dispersed to perform second stage encapsulation. The internal temperature was rapidly lowered to obtain a dispersion of microcapsules containing amino acids as the core material. This microcapsule dispersion was filtered to remove the solvent, and the microcapsules were washed with ethyl alcohol and dried, resulting in a particle size of 14.
Amino acid-containing microcapsules with relatively uniform amino acids in the range of 0 to 400p were obtained.

[Example 2] Microcapsules were obtained in the same manner as in Example 1, except that the mixture of hardened beef tallow and amino acids was directly dispersed in propylene gelcol without performing the first stage microcapsule formation.

[Example 3] Microcapsules were obtained in the same manner as in Example 1, except that the temperature of the encapsulation medium (propylene gelcol) in the second stage encapsulation was changed from 90°C to 70°C.

[Example 4] Cured beef tallow and beef tallow (50%
:50. Microcapsules were obtained in the same manner as in Example 1, except that a mixed fat and oil having a weight ratio (the same applies hereinafter) was used.

[Example 5] Hydrogenated beef tallow and soybean oil (6
Microcapsules were obtained in the same manner as in Example 1, except that a mixed oil and fat of 0:40) was used.

[Example 6] Microcapsules were obtained in the same manner as in Example 1, except that a mixed fat of palm stearin and soybean oil (60:40) was used instead of hardened beef tallow as the membrane material.

[Example 7] Except that ethylene glycol was used instead of propylene gelcol as the first and second stage encapsulation media.
Microcapsules were obtained in the same manner as in Example 1.

[Comparative Example 1] Example 1 except that glycerin was used instead of propylene gelcol as the first and second stage encapsulation media.
Microcapsules were obtained in the same manner.

[Comparative Example 2] Microcapsules were obtained in the same manner as in Example 1, except that a mixed solvent of glycerin:methanol (2:1) was used instead of propylene gelcol as the first and second stage encapsulation media. .

Table 1 lists the materials used and reaction temperature conditions for each side, and also shows the test results of the obtained microcapsules.

The microcapsule test was conducted as follows.

〔Test method〕

The test solution was stirred and uniformly dispersed and collected on a slide glass, and the following items were observed using a microscope.

(1) Encapsulation rate The percentage of core substances completely encapsulated in fats and oils.

(2) Amino acid survival rate Percentage of core material dispersed in the solvent without encapsulation.

The above am was performed three times on the same sample, and the average value was used as the result.

From the results in Table 1, it is clear that according to the production method of the present invention using propylene gelcol or ethylene glycol as the encapsulation medium, amino acids and/or peptides containing amino acids and/or peptides in good condition with no dissolution of membrane substances or elution of core substances. It was confirmed that oil and fat microcapsules were obtained.

[Example 8] Microcapsules were obtained in the same manner as in Example 1, except that tetrapropylene gelcol was used instead of propylene gelcol as the encapsulation medium in the first and second stages.

[Example 9] Microcapsules were obtained in the same manner as in Example 1 except that the amino acid as the core substance was replaced with glutamylglutamine.

The capsules of Examples 8 and 9 above are both of the capsules of Example 7 above.
had similar results.

Applicant: Laion Co., Ltd. Agent: Patent Attorney Takashi Kojima

Claims (1)

[Claims] 1. After dispersing one or more core substances selected from amino acids, dipeptides, tripeptides, and salts thereof in fats and oils, the core substance dispersion slurry has a molecular weight of 60 to 400.
A method for producing microcapsules, which comprises adding and dispersing the core substance in alkylene glycol to obtain microcapsules in which the core substance is coated with oil or fat.