JP6864279B1 - Information collection method, information collection system and information collection device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an information collecting method, an information collecting system and an information collecting device capable of grasping how a person's living condition is adversely affecting the person as well as the health condition of the subject by a simpler method. SOLUTION: A disintegration step of disintegrating the erythrocyte membrane of red blood cells contained in blood collected from a subject, a post-disintegration observation step of observing the state of blood after the disintegration step, and a post-disintegration observation step of blood observed An information collection method including a collection step of collecting information for grasping at least one of a subject's health condition and living condition based on the condition. [Selection diagram] Fig. 1

Description

The present invention relates to an information collecting method, an information collecting system, and an information collecting device.

Conventionally, a health examination for examining the health condition of a subject has been performed from the viewpoint of preventing or detecting a disease. In the health examination, for example, there is an item in which blood is collected from a subject and the components of the blood are inspected. In addition, the blood may be observed with a microscope or the like, thereby observing the state of the blood.

Patent Document 1 describes a blood test method. In this blood test method, the subject's left hand to the first joint are disinfected, the subject himself pierces the disinfection site with a lancet needle to bleed, and immediately puts a ball-shaped trace amount of blood on the underside of the cover glass and slides the glass. It is sandwiched between the upper surface and the upper surface of the. Then, the sample slide is set in a microscope, the focus is adjusted to a high magnification using oil for oil immersion, and the point of the visual field is set at the place where the red blood cells are distributed to the extent that they maintain the same three-dimensional structure and do not overlap, and the pattern is observed. I do. Further, the points of the visual field are sequentially shifted in the horizontal and vertical directions to observe patterns such as blood cells each time, and then the whole observation of the foreign matter in plasma is performed at a low magnification, and the foreign matter in plasma is again made high magnification. The observation of is performed in a slightly different place by the same process. Then, enter these results in the itemized checklist.

Patent Document 2 describes a blood health support system. In this blood health support system, a plurality of frame images extracted from a moving image captured from a phase-contrast microscope are subjected to difference processing for background elimination, binarization processing for converting a grayscale image into a binary image, and noise removal. The contraction / expansion process of the image is performed as a preprocessing. After that, a label for identifying each cell in the frame image is attached to the same cell by comparing the frame images before and after, and the locus of each cell is obtained. In addition, for one frame image, features such as the area of cells in the still image, the perimeter, the vertical or horizontal length of the circumscribed rectangle, the number of holes, the hole area, and the complexity from a perfect circle are measured. .. The characteristics of the cells thus obtained are compared with the characteristics of the cells registered in advance, and the cell state or the health state based on the cell state is determined.

JP-A-2002-55101 Japanese Unexamined Patent Publication No. 2005-345310

From the viewpoint of prevention and detection of diseases, it is preferable to carry out health examinations more times to understand the state of health.
However, with the conventional method, it is difficult to perform frequent medical examinations from the viewpoint of time, cost, physical burden, and the like.
Further, for example, in a blood test, if the numerical value of the analysis result is within the range of the specified value, it is considered normal regardless of whether it is close to the upper limit or the lower limit, and even if a physical abnormality is complained, the test may not determine the abnormality.
The present invention aims to provide an information collecting method, an information collecting system, and an information collecting device capable of grasping how a person's living condition is adversely affecting the person as well as the health condition of the subject by a simpler method. It is a thing.

Thus, according to the present invention, observation is performed in a disintegration step of disintegrating the erythrocyte membrane of erythrocytes contained in blood collected from a subject, a post-disintegration observation step of observing the state of blood after the disintegration step, and a post-disintegration observation step. An information collection method including an information collection step for collecting information for grasping at least one of a subject's health condition and living condition based on the blood condition obtained is provided.
Here, the pre-disintegration observation step of observing the state of red blood cells contained in the blood before the disintegration step is further included, and the information gathering step further includes the blood state observed in the pre-disintegration observation step to obtain the health condition of the subject and the subject's health condition. It is possible to collect information that grasps at least one of the living conditions. In this case, more information can be collected about the condition of the blood.
In addition, in the pre-collapse observation step, the shape of red blood cells in blood can be observed. In this case, the state of blood can be grasped more easily.
Furthermore, in the pre-collapse observation step, Brownian motion of particles other than erythrocytes can be observed. In this case, the state of blood can be further grasped by particles other than red blood cells.
Furthermore, the post-collapse observation step can be performed a plurality of times at predetermined time intervals after the disintegration step. In this case, the state of blood can be grasped by the time course of blood after pressing.
Then, in the post-disintegration observation step, the degree of erythrocyte disintegration due to the disintegration step can be observed in a state where there is no change with time. In this case, the fragility of red blood cells makes it possible to grasp at least one of the health condition and living condition of the subject.
In addition, the post-collapse observation step can be made to observe the internal substance of the red blood cells that have collapsed in the disintegration step. In this case, the internal substance of red blood cells can be used to grasp at least one of the health condition and living condition of the subject.
Further, the post-collapse observation step can be made to further observe the morphological change of the internal substance and the shape change of the particles of the internal substance after a predetermined time after the disintegration step. In this case, the state of blood can be grasped by the time course of the internal substance of blood after pressing.
Furthermore, the post-collapse observation step can further observe the presence or absence of the appearance of agglomerated cytoplasm produced from the internal substance after a predetermined time after the disintegration step. In this case, the state of blood can be grasped by the time course of the internal substance of blood after pressing.

Further, according to the present invention, a disintegrating means for disrupting the erythrocyte membrane of erythrocytes contained in blood collected from a subject, an observing means for observing the state of blood after disintegration by the disintegrating means, and blood observed by the observing means. An information collecting system including an information collecting means for collecting information for grasping at least one of a subject's health state and living state based on the state of the subject is provided.
Here, the observing means further observes the state of red blood cells contained in the blood before the disintegration by the disintegrating means, and the information collecting means further includes the state of the blood observed before the disintegration, and the health state and living state of the subject. It is possible to collect information that grasps at least one of the above.

Further, according to the present invention, an acquisition unit that acquires an image of blood after disrupting the erythrocyte membrane of erythrocytes contained in blood collected from a subject at predetermined time intervals, and an acquisition unit acquired by the acquisition unit. An information collecting device including an information collecting unit for collecting information for grasping at least one of a subject's health condition and living condition based on an image is provided.

According to the present invention, it is possible to provide an information collecting method and an information collecting device capable of grasping at least one of a subject's health condition and living condition by a simpler method.

It is a flowchart explaining the information gathering method of this embodiment. (A) to (E) are diagrams showing the state of blood in the pre-collapse observation step. It is a figure which showed the state of blood immediately after disintegration. (A) to (F) are diagrams showing the state of blood 20 to 24 hours after disintegration. It is a figure which showed the state of blood 20 hours to 24 hours after the disintegration. It is a figure which showed the state of blood 40 hours to 48 hours after the disintegration. It is a figure which showed the information gathering system of this embodiment. It is a block diagram which showed the functional configuration example of an analyzer. It is a flowchart explaining the operation of the analyzer.

Hereinafter, embodiments for carrying out the present invention (hereinafter, embodiments of the present invention) will be described in detail. The present invention is not limited to the following embodiments, and can be variously modified and implemented within the scope of the gist thereof. In addition, the drawings used are for explaining the present embodiment and do not represent the actual size.
Hereinafter, the information collection method and the like to which the present embodiment is applied will be described based on the drawings.

<Overall explanation of information collection method>
First, the information collecting method of the present embodiment will be described.
FIG. 1 is a flowchart illustrating a method of collecting information according to the present embodiment.
First, the necessary equipment is prepared (step 101: preparation step). Here, first, a microscope is prepared.
The microscopes to be prepared are limited to those that do not require treatment such as staining that causes scientific changes in the collected blood and that the collected blood can be observed raw, and at least the magnification is 80 μm inside or outside the size of red blood cells. It is necessary to be able to observe both the particles of 100 nm and the particles inside and outside 100 nm contained in the internal substance.
In addition, prepare a slide glass and a cover glass. The cover glass may be, for example, a small one of 18 mm square or less.

Next, the blood of the subject whose information is to be collected is collected (step 102: collection step).
Blood sampling is also called blood sampling. The method of collecting blood is not particularly limited, and a method of using an existing blood collection puncture tool, a method of using a syringe, or the like can be adopted. The amount of blood collected in the present embodiment is an amount that can be observed later, and for example, one droplet of blood is sufficient. The volume of blood is, for example, about 0.1 mL. That is, in the information collecting method of the present embodiment, the amount of blood used may be a small amount, and the burden on the subject can be reduced.

Next, blood is attached to the cover glass and the slide glass is allowed to stand to prepare a preparation (step 103: preparation step). On the contrary, a preparation may be prepared by dropping blood on a slide glass and covering it with a cover glass.
Then, using a microscope, the blood before the erythrocyte membrane is disrupted is observed (step 104). Here, the red blood cells contained in the blood sample are observed.
Then, information for grasping at least one of the health condition and living condition of the subject is collected (step 105: information collection step before collapse). The specific contents of the information gathering process before the collapse will be described later.

Further, by pressing the blood collected from the subject, the erythrocyte membrane of the erythrocytes contained in the blood is disrupted (step 106: disruption step). In the disintegration step, the blood on the slide glass is crushed with a cover glass to disintegrate as many red blood cells as possible. It is also possible to apply a predetermined stress to the cover glass. For example, by placing a weight having a predetermined weight on the cover glass, a predetermined stress is applied to the cover glass.
Further, the disintegration step is not limited to the case where erythrocytes are disintegrated by pressing. For example, by moving and rubbing at least one of the slide glass and the cover glass, the erythrocyte membrane may be disrupted by the shearing force acting between the two. The important point is not to chemically react with the erythrocyte membrane. Also, it is not necessary to disrupt the erythrocyte membrane of all erythrocytes in the preparation.

Then, the blood immediately after disintegration is observed using a microscope (step 107: first observation step after disintegration).
Further, information for grasping at least one of the health condition and living condition of the subject immediately after the collapse is collected (step 108: first information collection step after the collapse).
Then, the blood is sealed with plastic wrap or the like so that the water content of the blood does not evaporate, and the mixture is left at room temperature for 20 to 24 hours (step 109: first standing step). In the first static step, it is not necessary to block oxygen. In addition, it is not necessary to sterilize or heat it to remove water, and the storage condition may be such that it is sealed with a wrap. The same applies to the second standing step and the third standing step described below.

Then, again, the blood is observed after 20 to 24 hours using a microscope (step 110: second observation step after disintegration).
Further, after 20 to 24 hours have passed, information for grasping at least one of the health condition and living condition of the subject is collected (step 111: second information collection step after collapse).
Next, the blood is sealed with plastic wrap or the like so that the water content of the blood does not evaporate, and the mixture is left at room temperature for 20 to 24 hours (step 112: second standing step). At this point, 40 to 48 hours have passed since the collapse.

Then, again, the blood is observed after 40 to 48 hours using a microscope (step 113: third observation step after disintegration).
Further, after 40 to 48 hours have passed, information for grasping at least one of the health condition and living condition of the subject is collected (step 114: third information collection step after collapse).
Next, the blood is sealed with plastic wrap or the like so that the water content of the blood does not evaporate, and the mixture is left at room temperature for 20 to 24 hours (step 115: third standing step). At this point, 60 to 72 hours have passed since the collapse.

Then, again, the blood is observed after 60 to 72 hours using a microscope (step 116: fourth observation step after disintegration).
Further, after 60 to 72 hours have passed, information for grasping at least one of the health condition and living condition of the subject is collected (step 117: fourth information collection step after collapse).

That is, in the present embodiment, after the erythrocyte membrane of the erythrocytes contained in the blood is disrupted by pressing or the like, a total of four observations of step 107, step 110, step 113, and step 116 are performed. These four observations can be regarded as a post-collapse observation step for observing the state of blood after the disintegration step.
Then, in the present embodiment, after the erythrocyte membrane of the erythrocytes contained in the blood is disrupted by pressing or the like, information is collected a total of four times in steps 108, 111, 114, and 117. In the first information gathering step after the collapse to the fourth information gathering step after the collapse, at least one of the health condition and the living condition of the subject is grasped based on the blood condition observed in the post-collapse observation step. It can be regarded as an information collection process for collecting information.
Further, before the erythrocyte membrane of the erythrocytes contained in the blood is disrupted by pressing or the like, one observation is performed in step 104. Step 104 is an observation step performed before the disintegration step of step 106, and can be regarded as a pre-disintegration observation step for observing the state of red blood cells contained in blood before the disintegration step.
Then, information for grasping at least one of the subject's health condition and living condition is collected, including the blood condition observed in the pre-collapse observation step. This information gathering process before the collapse can also be regarded as an information gathering process. The contents of the information collected in these information collection steps will be described later.

<Explanation of pre-collapse observation process and pre-collapse information collection process>
Next, the pre-collapse observation step and the pre-collapse information gathering step will be described in more detail.
Here, the description will be given with reference to FIGS. 2 (A) to 2 (E). 2 (A) to 2 (E) are views showing the state of blood in the pre-collapse observation step.
In the pre-collapse observation step, the blood condition is observed based on the following criteria (1A) to (1F). Then, in the information gathering process before the collapse, information for grasping at least one of the health condition and the living condition of the subject is collected based on these judgment criteria. Here, in (1A) to (1D), the shape of red blood cells S in blood is observed. Further, in (1E) to (1F), Brownian motion of particles other than erythrocyte S is observed.

(1A) As shown in FIG. 2A, there is no problem as long as the shape of the red blood cells S is close to a perfect circle.
(1B) As shown in FIG. 2B, even if the erythrocytes S are connected like an abacus ball and form a chain, there is no problem as long as the erythrocytes are close to a perfect circle.
(1C) As shown in Fig. 2 (C), if some red blood cells S have irregularities that are not perfect circles or red blood cells S with protrusions in some places, unbalanced diet, ingestion of processed foods, and irregular life And there may be a lot of stress.
(1D) As shown in FIG. 2D, when most of the red blood cells S have a squid-like shape, the blood collection condition may be poor, so blood is collected again to prepare a slide. If there are a large number of red blood cells S with mussel even after blood is collected again, it is possible that there is stress or some kind of disease.
(1E) As shown in FIG. 2 (E), in addition to the above-mentioned states (1A) to (1D), when a large amount of granular small molecular substance L other than erythrocyte S is observed to undergo Brownian motion, In many cases, supplements, medicines, or vitamin injections are taken.
(1F) It is normal if the activity of the movement of the molecule L other than the erythrocyte S is active with a vigorous movement. On the other hand, if the movement is sluggish, it is possible that the person is in poor physical condition. In addition, if it is more intense than necessary, artificial synthetic molecules may be involved.

<Explanation of post-collapse observation process and post-collapse information collection process>
Next, the post-collapse observation step and the post-collapse information gathering step will be described in more detail. As described above, the post-collapse observation step is performed a plurality of times at predetermined time intervals after the collapse step. Then, in the post-collapse observation step, the state of blood is observed based on the following criteria. Then, in the information collection process after the collapse, information for grasping at least one of the health condition and the living condition of the subject is collected based on these judgment criteria.

(Immediately after collapse)
Here, the description will be given with reference to FIG. FIG. 3 is a diagram showing the state of blood immediately after disintegration.

(2A) When most of the erythrocyte S does not easily disintegrate, the erythrocyte membrane is highly elastic and flexible. That is, the subject is in a state where he / she is healthy and can maintain a reasonable youth.
(2B) When most of the erythrocyte S is easily disintegrated, it is considered that the protein membrane, which is the erythrocyte membrane, is deteriorated, that is, there are many oxidative factors in the subject's daily living environment and food.
(2C) As shown in FIG. 4 to be described later, the molecular substance B, which is the content produced by the decay of the erythrocyte S, has a normal size of about 100 nm, but as shown in FIG. In rare cases, a molecular substance C several times larger than this may be seen. This molecular substance C has, for example, a size of 150 nm or more and 10 μm or less. In that case, there are many cases in which a large amount of artificially synthesized molecules (synthetic additives, etc.) are eaten, or there is some kind of poor quality lifestyle.
(2D) Similar to the judgment in (2C) above, it is normal if the movement activity of the molecules is active, but if the movement is slow, poor physical condition is considered. On the other hand, if you exercise more violently than necessary, you may be eating a lot of additives or taking medicines.

(After 20 to 24 hours)
Here, the description will be given with reference to FIGS. 4 (A) to 4 (F) and FIG. 4 (A) to 4 (F) and 5 are diagrams showing the state of blood 20 to 24 hours after the disintegration.

Here, first, the following observations (3A) to (3C) are performed.
(3A) As shown in FIGS. 4 (A) to 4 (C), if a granular molecule having a size of about 100 nm exhibits a chain such as a chain or an octopus foot like the molecule B, the molecule The anomaly is small and there is no problem with the quality of life. It is believed that this is produced by the influence of the contents of the collapsed erythrocytes S. It can be understood that this chain of molecular substances B is formed as a result of the shape change of the particles of the internal substance.
(3B) As shown in FIG. 4 (D), when there is a corona-like, sunflower-like, sun-like, aggregate of molecular B that is slightly smaller than the erythrocyte S, a large amount of an artificial synthetic additive or the like is consumed. There are many. However, if the above-mentioned octopus-foot-shaped chains are seen at the same time, there is almost no problem.
(3C) If, as shown in FIG. 4 (E), a molecule B such as a rod-shaped microorganism appears, or if the visual field is cloudy as shown in FIG. 4 (F), is the quality of life poor? It is assumed that there is a disease.

Further, the following observations (4A) to (4C) are performed.
(4A) As shown in FIG. 5, if cytoplasm T such as a mass of protein appears in the visual field, the health condition is good. It is thought that this is produced by changing the collapsed red blood cells S and internal substances and internal particles.
(4B) One of the causes when cytoplasm T such as a mass of protein is not formed in the visual field is due to old age. In the case of elderly people, reconfirm the situation after 40 to 48 hours. Adolescents and young people without formation often have poor eating habits, poor living conditions, stress, or poor physical condition, so reconfirm after a while.
(4C) If there is almost no cytoplasmic T such as a mass of protein in the visual field, the body has a poor diet, poor living conditions, stress, or some adverse effects. Or, you often have some kind of illness such as getting tired easily, so check again after a while.

(After 40 to 48 hours have passed)
Here, the description will be given with reference to FIG. FIG. 6 is a diagram showing the state of blood 40 to 48 hours after the disintegration.

(5A) As shown in FIG. 6, if almost all the granular contents having a size of 100 nm have stopped exercising and are replaced with a large but protein-lumpy cytoplasm T in the screen, there is a problem with the health condition. There is no. If there is no health problem, this is the end of the observation.
(5B) If the protein mass cytoplasm T is hardly formed or the molecules continue to move, the state after the next 60 to 72 hours is reconfirmed.

(After 60 to 72 hours)
(6A) If the protein mass cytoplasm T is not formed up to this point, it is apparently a metabolic disorder, so a detailed interview will be conducted and guidance will be given to improve life.
(6B) If the Brownian motion of a molecule with a size of 100 nm continues, it is possible that artificial synthetic molecules (pharmaceuticals, poor quality supplements, cosmetics, etc.) are ingested. Guidance on life improvement, such as letting them do it.

In the post-disintegration observation step, immediately after blood collection, the degree of disintegration of erythrocytes S due to the disintegration step is observed without any change with time. Further, in the post-collapse observation step, the internal substance of the erythrocyte S that has collapsed in the disintegration step is observed. At this time, the morphological change of the internal substance and the shape change of the particles of the internal substance are observed. In addition, the presence or absence of the appearance of massive cytoplasm T produced from the internal substance is further observed after a predetermined time after the disintegration step.

<Overall explanation of information collection system 1>
Next, the information collection system 1 of the present embodiment will be described.
FIG. 7 is a diagram showing the information collection system 1 of the present embodiment.
The illustrated information gathering system 1 includes a microscope 10, a slide 20, and an analyzer 30.
The microscope 10 is an optical microscope, for example, a phase-contrast microscope or a dark-field microscope. The microscope 10 is an example of an observation means for observing the state of blood.
The microscope 10 is, for example, a phase-contrast microscope or a dark-field microscope, but is not particularly limited. For example, it may be a digital microscope that is connected to a PC (Personal Computer), a smartphone, a tablet, or the like and displays an information collection target on a display.

The microscope 10 irradiates the preparation 20 placed on the stage 11 with the light emitted from the light source 12 through the condenser lens 13, and forms an image of the transmitted light transmitted through the preparation 20 with the objective lens 14 and the eyepiece lens 15. .. When the microscope 10 is a phase-contrast microscope, the objective lens 14 is an objective lens with a phase plate. When the microscope 10 is a dark field microscope, the condenser lens 13 is provided with a light-shielding plate. Then, by observing the formed optical image with the eyepiece lens 15, blood, which is a sample prepared as a slide, can be observed. Further, the microscope 10 can guide the transmitted light to the CCD (Charge Coupled Devices) image sensor 16 to form an image as an optical image. Then, the CCD image sensor 16 photoelectrically converts the optical image. That is, the CCD image sensor 16 photoelectrically converts the transmitted light transmitted through the slide on a pixel-by-pixel basis, and outputs R (red), G (green), and B (blue) analog image signals.

The preparation 20 is prepared by sandwiching blood as a sample between a slide glass and a cover glass. Further, in the present embodiment, as described above, the blood on the slide glass is crushed or rubbed with the cover glass to disintegrate the red blood cells. Therefore, in this case, the slide glass or the cover glass functions as a disintegrating means for disintegrating the erythrocyte membrane of the erythrocytes contained in the blood collected from the subject.

The analyzer 30 is an example of an information collecting device or an information collecting means, and collects information for grasping at least one of a subject's health condition and living condition based on the blood condition observed by the microscope 10.
In this case, the analyzer 30 acquires an analog image signal output from the microscope 10, and based on the image formed based on the analog image signal, information for grasping at least one of the health condition and the living condition of the subject. To collect.

The analyzer 30 is, for example, a PC. Further, it may be a mobile terminal such as a mobile computer, a mobile phone, a smartphone, or a tablet. The analyzer 30 performs wired communication or wireless communication and connects to the microscope 10. Further, the microscope 10 may be connected via a LAN (Local Area Network) or a WAN (Wide Area Network). Further, the analyzer 30 may be connected to the microscope 10 via a plurality of networks or communication lines by using a relay device such as a gateway device or a router.

The analyzer 30 includes a CPU (Central Processing Unit) which is a calculation means, a main memory which is a storage means, and storage such as an HDD (Hard Disk Drive) and an SSD (Solid State Drive) which are auxiliary storage means. The CPU executes various software such as OS (basic software) and application software (application software). The main memory is a storage area for storing various software and data used for executing the software, and the storage is a storage area for storing input data for various software and output data from various software.
Further, the analyzer 30 includes a communication interface for communicating with the outside (hereinafter referred to as “communication I / F”), a display device including a video memory and a display, and an input device such as a keyboard and a mouse. And.

FIG. 8 is a block diagram showing a functional configuration example of the analyzer 30.
Here, among various functions of the analyzer 30, those related to the present embodiment are selected and shown.

The analyzer 30 includes a transmission / reception unit 310 for transmitting / receiving information to / from an external device, a display unit 320 for displaying an image, an input unit 330 for inputting an instruction by a user, a storage unit 340 for storing information, and an analyzer. A control unit 350 for controlling and analyzing the entire 30 is provided.

The transmission / reception unit 310 acquires an analog image signal of blood as a sample from the microscope 10. The transmission / reception unit 310 corresponds to, for example, a communication I / F.
The transmission / reception unit 310 functions as an acquisition unit that acquires an image of blood after disrupting the erythrocyte membrane of red blood cells contained in blood collected from a subject at predetermined time intervals.

The display unit 320 displays an image of blood. The display unit 320 corresponds to a display device such as a display, for example.
The input unit 330 is a mechanism unit for inputting necessary items by the user when observing blood. The input unit 330 corresponds to, for example, an input device.
The display device and the input device may be a touch panel or the like having these functions.
The storage unit 340 stores image information acquired from the microscope 10 and the result of analysis by the control unit 350. The storage unit 340 corresponds to, for example, storage.

The control unit 350 is an example of the information collection unit, and collects information for grasping at least one of the health condition and the living condition of the subject based on the image acquired by the transmission / reception unit 310. In this case, the control unit 350 acquires the state of blood as a sample by performing image processing, and uses this as information for grasping at least one of the health state and living condition of the subject.

Specifically, the state of blood to be acquired is the shape of red blood cells according to the above-mentioned criteria (1A) to (1D) in the pre-collapse observation step. Further, in the above-mentioned judgment criteria (1E) to (1F), it is the motion of Brownian motion particles other than erythrocytes.
The control unit 350 can acquire the state of blood by image processing. Specifically, the control unit 350 extracts the outer edge portion of the particles existing in the image. Then, the control unit 350 can perform pattern matching between the shape of the outer edge portion and the shape prepared in advance, and determine the shape of the red blood cells. Further, the control unit 350 can distinguish between red blood cells and other particles according to the size of the outer edge portion, and further, the control unit 350 can distinguish the outer edge portion at predetermined time intervals. By detecting the position of, the movement of red blood cells and other particles can be grasped.

The state of blood to be acquired is the degree of erythrocyte disintegration due to the disintegration step described in the above-mentioned criteria (2A) to (2B) immediately after disintegration in the post-disintegration observation step. Then, it is an internal substance of red blood cells that has been destroyed by pressing or the like described in the above-mentioned judgment criteria (2C) to (2D). In the former case, the control unit 350 can determine the degree of erythrocyte decay by counting the number of remaining erythrocytes. In the latter case, the outer edge of the internal substance can be extracted, and the shape and motion can be grasped from this.

Then, after 20 hours to 24 hours, 40 hours to 48 hours, and 60 hours to 72 hours, the above-mentioned criteria (3A) to (3C), (4A) to (4C), (5A) It is a morphological change of an internal substance internal substance such as a mass of a protein and a shape change of a particle of the internal substance described in (5B), (6A) to (6B). It is also the movement of internal matter. These can extract the outer edge of the internal substance and grasp the shape and movement from it.

<Explanation of operation of analyzer 30>
Next, the operation of the analyzer 30 will be described.
FIG. 9 is a flowchart illustrating the operation of the analyzer 30.
First, the control unit 350 determines whether or not there is an instruction from the user (step 201). This instruction is an instruction to collect information for grasping at least one of the health condition and the living condition of the subject in the above-mentioned information collecting process. That is, the user gives this instruction at a predetermined timing in the pre-collapse observation step and the post-collapse observation step. This instruction is given, for example, by the user operating the input unit 330.
As a result, if no instruction is given (No in step 201), the process returns to step 201.
On the other hand, when instructed (Yes in step 201), the control unit 350 acquires an image of blood from the microscope 10 via the transmission / reception unit 310 (step 202).

Next, the control unit 350 displays this image on the display unit 320 (step 203). Further, the control unit 350 stores this image in the storage unit 340 (step 204).
Then, the control unit 350 performs image processing of the blood image and collects information for grasping at least one of the health condition and the living condition of the subject (step 205).
The control unit 350 displays this result on the display unit 320 (step 206). At this time, at least one of the health condition and the living condition of the subject is displayed based on the above-mentioned judgment criteria.
Further, the control unit 350 stores the result in the storage unit 340 (step 207).
It should be noted that the observation for each passage of time may be automatically operated by setting a timer instead of manually. Further, the microscope 10 may be integrated with the analyzer 30.

Health diagnosis is an important method from the viewpoint of disease prevention and detection.
Municipalities and companies carry out annual health examinations and human docks, aiming for early treatment.

In the past, the mortality rate of demencia senil was high, but after the war, cancer increased, and since the 1990s, diseases called lifestyle-related diseases, which are caused by the quality of daily life, have increased. There are chronic diseases such as hypertension and gout that are not easily linked to death, but diseases that develop into artificial dialysis such as diabetes and diseases that progress to recurrence or systemic metastasis even if they are cured once, such as cancer, are increasing. Therefore, the burden of medical expenses in the country has become a big problem.

However, prevention of illness and medical examinations and tests for detecting illness are time-consuming and costly, and depending on the content of the test, the burden on the body is heavy, and it is not easy to carry out the test frequently. Under these circumstances, if the physical condition can be checked more easily and the person undergoing the test can feel the badness of life by checking it by himself / herself, it can be expected that the improvement of life will be further promoted.
Moreover, if any factor that causes some kind of illness is found in the future, it is possible to immediately improve the life and greatly contribute to suppressing the outbreak of illness in society.

In addition, it is difficult for doctors to diagnose the pre-illness condition called non-illness, and even if you have a physical condition that is not a disease, there is no reliable diagnosis method, and some people do not understand the pain.
It is possible to judge the living conditions to be grasped here, for example, that the supplements that the person is taking are not suitable for the person and are rather harmful, and can be grasped by the conventional health examination. You will be able to easily determine the cause of health problems that you could not do.
If such physical condition can be easily confirmed, more people will be able to live without anxiety.

Then, according to the information collecting method, the information collecting system 1 and the analyzer 30 described in detail above, the red blood cells are disrupted by pressing the blood or the like, and the change over time in the blood state after the disruption is observed, and the health condition of the subject is observed. And get information to understand at least one of the living conditions. Specifically, the degree of erythrocytes that disintegrate due to pressing or the like is observed. In addition, the internal substances of the collapsed erythrocytes are observed, and the morphological change, shape change, and appearance of particles generated from the influence of the internal substances are observed. Thereby, at least one of the health condition and the living condition of the subject can be grasped by a simpler method.

At the same time, observe the state of erythrocytes before they collapse by pressing blood and the movement of Brownian-motion particles other than erythrocytes, and acquire information for grasping at least one of the health condition and living condition of the subject. .. This makes it possible to grasp the state of red blood cells of the subject and Brownian motion particles other than red blood cells, and to grasp at least one of the health state and living condition of the subject in more detail.

In addition, the equipment used in the information gathering method described in detail above is not expensive at all. For example, as the microscope 10, a general-purpose microscope such as a phase-contrast microscope or a dark-field microscope can be used, and as the analyzer 30, a normal PC can be used. Moreover, it is of course inexpensive to prepare the slide 20. That is, no clinical equipment or clinical test such as blood culture, urinalysis, stool test, etc. is required. Furthermore, the amount of blood used may be very small, and there is almost no burden on the subject.

Therefore, the above information gathering method and the like can be implemented in most private hospitals in municipalities, for example, and can be used as a technique in which doctors called family doctors diligently support the physical condition management of local people on a daily basis. As a result, it can contribute to the prevention and detection of diseases.
In addition, the above information collection method, etc., should be treated hygienically by people who are qualified to protect people's health, such as acupuncturists, chiropractors, public health nurses, and health managers, even if they are not doctors. It can be used without problems.

Furthermore, as a result of grasping at least one of the health condition and the living condition by the above information gathering method etc., most of the lifestyle improvement performed by the subject is due to the improvement of eating habits and the attitude of daily life, so that the subject can easily work on it. There is no need for expensive medicines. And if you can grasp your physical condition at an early stage and improve your health by improving your life, it will lead to a reduction in the burden of medical expenses in the country.
In addition, by applying the information gathering method described in detail above, it is possible to contribute to drug development as a simple, shorter-term, and safer judgment method for the effect of a new drug on the health condition.

1 ... Information collection system, 10 ... Microscope, 20 ... Preparation, 30 ... Analyzer, 310 ... Transmission / reception unit, 320 ... Display unit, 330 ... Input unit, 340 ... Storage unit, 350 ... Control unit

Claims (12)

The disintegration process that disrupts the red blood cell membrane of red blood cells contained in the blood collected from the subject,
A post-disintegration observation step for observing the state of blood after the disintegration step,
An information collection step of collecting information for grasping at least one of the health condition and the living condition of the subject based on the blood condition observed in the post-collapse observation step.
Information gathering methods including. The pre-disintegration observation step of observing the state of red blood cells contained in the blood before the disintegration step is further included.
The information gathering step according to claim 1, further comprising collecting information for grasping at least one of the health state and the living state of the subject, including the blood state observed in the pre-collapse observation step. Information gathering method. The information collecting method according to claim 2, wherein the pre-collapse observation step is for observing the shape of red blood cells in blood. The information gathering method according to claim 3, wherein the pre-collapse observation step is for observing Brownian motion of particles other than erythrocytes. The information collecting method according to claim 1, wherein the post-collapse observation step is performed a plurality of times at predetermined time intervals after the collapse step. The disintegration after observation step, the degree of disintegration of red blood cells by the disintegration process, the information collection method according to claim 1, characterized in that observed in the absence of aging. The information collecting method according to claim 5, wherein the post-disintegration observation step observes an internal substance of red blood cells that have collapsed in the disintegration step. The post-collapse observation step according to claim 7, wherein the post-collapse observation step further observes the morphological change of the internal substance and the shape change of the particles of the internal substance after a predetermined time after the disintegration step. Information gathering method. The information according to claim 7, wherein the post-collapse observation step further observes the appearance of a mass cytoplasm produced from the internal substance after a predetermined time after the disintegration step. Collection method. Disintegration means that disrupts the red blood cell membrane of red blood cells contained in blood collected from the subject,
An observation means for observing the state of blood after disintegration by the disintegration means,
An information collecting means for collecting information for grasping at least one of the health condition and the living condition of the subject based on the blood condition observed by the observation means.
Information gathering system equipped with. The observing means further observes the state of red blood cells contained in the blood before the disintegration by the disintegrating means.
The information collecting system according to claim 10, wherein the information collecting means collects information for grasping at least one of a subject's health condition and living condition, including the blood condition observed before the collapse. .. An acquisition unit that acquires images of blood after disrupting the erythrocyte membrane of red blood cells contained in blood collected from a subject at predetermined time intervals, and an acquisition unit.
Based on the image acquired by the acquisition unit, an information collection unit that collects information for grasping at least one of the health condition and the living condition of the subject, and an information collection unit.
An information gathering device equipped with.

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