JP4894039B2 - Biosensor cartridge and biosensor device - Google Patents

Description

  The present invention relates to a biosensor cartridge, for example, a biosensor cartridge and a biosensor device that measure and analyze a chemical substance using a reagent contained in a hollow reaction part of a chip.

Conventionally, for example, a biosensor chip that detects the concentration of glucose in blood is known (see, for example, Patent Document 1).
FIG. 6 is an exploded perspective view showing the glucose sensor described in Patent Document 1. As shown in FIG. As shown in FIG. 6, a glucose sensor 100 that is a biosensor has a counter electrode 101 and a working electrode 102. The counter electrode 101 has a hollow needle shape half cut in the length direction, and a tip portion 103 thereof is obliquely cut into an injection needle shape so as to be easily punctured. The cut surfaces that have been cut are generally coated with insulating layers 104 and 104 'that also serve as adhesive layers, such as epoxy resin adhesives, silicone adhesives, or glass. The insulating layers 104 and 104' The working electrode 102 is attached via The working electrode 102 is a flat plate member to which glucose oxidase (GOD) is immobilized, and is adhered to the counter electrode 101 with the so-called immobilized GOD 105 surface side to which the GOD is immobilized facing inward.
Therefore, blood is collected by puncturing the subject 103 with the tip 103 of the needle-shaped counter electrode 101, and the reaction between the collected blood and the immobilized GOD 105 is detected by the working electrode 102 to determine glucose.

In addition, a biosensor in which a biosensor chip and a lancet are integrated is disclosed (see, for example, Patent Document 2).
7A is a perspective view of the sensor described in Patent Document 2, and FIG. 7B is an exploded perspective view of the sensor. As shown in FIG. 7, the lancet-integrated sensor 110 includes a chip body 111, a lancet 113, and a protective cover 115. The chip body 111 has a cover 111a and a substrate 111b that can be opened and closed, and an internal space 112 is formed on the inner surface of the cover 111a. The internal space 112 has a shape that can accommodate the lancet 113 in a movable manner.

  The needle 114 provided at the tip of the lancet 113 can be projected and retracted from an opening 112 a formed at the front end of the internal space 112 of the chip body 111 as the lancet 113 moves. The shape of the internal space 111a is curved so that the width thereof is slightly narrower than that of the lancet 113 at the end where the protrusion 113a is located, and the lancet 113 is locked to the chip body 111 by mutual pressing force and frictional force. It is like that. The protective cover 115 has a tube portion 115 a into which the needle 114 is inserted, and the tube portion 115 a can be accommodated inside the chip body 111 as the needle 114 moves. Therefore, in a state before use, the protective cover 115 is put on the needle 114 to protect the needle 114 and prevent the user from being accidentally injured. Note that the substrate 111b is provided with a pair of electrode terminals 116 so that it can be electrically connected to a biosensor device (not shown).

In use, the protective cover 115 is removed and the lancet 113 is pushed to cause the needle 114 to protrude from the tip body 111. After puncturing the subject in this state, the needle 114 is housed inside the chip body 111, the opening 112a provided at the front end of the chip body 111 is brought close to the puncture port, and the outflowed blood is collected.
JP-A-2-120655 International Publication No. 02/056769 Pamphlet

However, in the glucose sensor 100 described in Patent Document 1, since the needle-like counter electrode 101 and the working electrode 102 are bonded to each other, the diameter of the puncture needle is approximately the same as the width of the glucose sensor 100 and increases. For this reason, there is a problem in that the amount of blood collected increases, pain during puncture increases, and the burden on the user increases.
In addition, the lancet-integrated sensor 110 described in Patent Document 2 has a structure that absorbs blood flowing out from the puncture port from the opening 112a, but the structure is complicated.

  The present invention has been made in view of the above-described problems, and an object of the present invention is to reduce the burden on the user by reducing the amount of sample collected for measurement and to bring the sample collection port closer to the puncture port. An object of the present invention is to provide a biosensor cartridge and a biosensor device that can not only take and measure a sample of a puncture opening easily without requiring an operation, but also enable more accurate measurement.

  In order to achieve the above-described object, a biosensor cartridge that is a first feature according to the present invention includes a biosensor chip having a sample collection port for collecting a sample at a tip, and is fixed to the biosensor chip. A biosensor cartridge having a puncture device, wherein the sample collection port is opened in a direction intersecting with an axis of the puncture device.

  In the biosensor cartridge configured as described above, the sample collection port can be made closer to the puncture port formed by the tip of the puncture device than before, so even a small amount of sample can be easily used. The sample can be collected by the sampling port and a reliable inspection can be performed.

According to a second aspect of the present invention, there is provided the biosensor cartridge according to the first aspect of the present invention, wherein the sample collection port is provided at a corner of the tip of the biosensor chip.
In the biosensor cartridge configured as described above, when the puncture device support and the biosensor chip are integrated, the sample collection port and the puncture device can be brought close to each other.
Further, it is preferable that an inclined angle portion is formed at the corner of the tip of the biosensor chip, and a sampling port is provided at the inclined angle portion.
By adopting this configuration, the sample collection port of the biosensor chip and the puncture device are brought closer to each other, and even a small amount of sample can be easily guided to the sample collection port.

The biosensor cartridge according to the third aspect of the present invention is the biosensor cartridge according to the first or second aspect of the present invention, wherein the puncture instrument support sandwiches the biosensor chip from the front and back. It is integrated with the biosensor chip.
In the biosensor cartridge configured as described above, the puncture device support and the biosensor chip can be easily combined and integrated, and the sample collection port and the tip of the biosensor chip can be easily arranged.

The biosensor cartridge according to the fourth feature of the present invention is the biosensor chip according to any one of the first to third features of the present invention, wherein the biosensor chip and the tip of the puncture device support are attached to the biosensor cartridge. An elastic body that includes a puncture opening formed in the subject by the tip of the puncture device and forms a space necessary for sampling is provided.
In the biosensor cartridge configured in this way, at the time of puncturing, the puncture port and the sample collection port provided at the tip of the biosensor chip are connected by a space formed by an elastic body, so even a small amount of sample can be used. A sample can be easily taken.
Further, according to this biosensor cartridge, it is possible to prevent the puncture device from protruding from the distal end surface of the elastic body before use, thereby protecting the puncture device and protecting the user. it can. Furthermore, it is possible to prevent the puncture device from protruding from the distal end surface of the elastic body when it is discarded after use, and thus it can be safely and properly disposed of.

  The biosensor cartridge according to the fifth feature of the present invention is the biosensor cartridge according to the fourth feature of the present invention, wherein at least the tip surface of the elastic body has adhesiveness.

In the biosensor cartridge configured as described above, since at least the distal end surface of the elastic body has adhesiveness, the surface in contact with the subject has adhesiveness, and the elastic body may be in close contact with the subject. It is possible to prevent the puncture position from shifting and to ensure the collection of the sample.
In addition, the elastic body is not limited to the case where only the tip surface has adhesiveness, but the elastic body itself has adhesiveness, the case where the adhesive is kneaded into the elastic body, the method of coating with the adhesive, etc. Is mentioned.

  A biosensor device according to a sixth feature of the present invention is obtained by connecting to the biosensor cartridge having any one of the first to fifth configurations of the present invention and a detection electrode of the biosensor cartridge. And a measuring instrument for obtaining information on the obtained sample.

  In the biosensor device configured as described above, since the sample is collected by the biosensor cartridge described above, the puncture and sample collection can be performed by a series of operations, and the sample collection port of the biosensor chip can be opened as in the past. It is not necessary to align with the puncture opening, and the sample can be collected easily and reliably. In addition, by transmitting the sample information to the measuring device via the detection electrode, the measurement can be easily performed in a short time, so that the burden on the subject can be reduced.

  According to the present invention, the sample collection port provided at one end of the biosensor chip and the axis of the puncture device are configured to intersect at an appropriate angle, so that the sample collection port is brought very close to the puncture port. Therefore, it is possible to provide a biosensor cartridge and a biosensor device that can easily collect a small amount of sample through the sample collection port and can perform a reliable test.

DESCRIPTION OF EMBODIMENTS Hereinafter, embodiments according to the present invention will be described in detail with reference to the drawings.
FIG. 1A is a longitudinal sectional view of a main part of an embodiment of the biosensor cartridge of the present invention as viewed from the side, and FIG. 1B is an elasticity in the embodiment of the biosensor cartridge of the present invention. It is the figure which removed the body and was seen from the front-end | tip direction (B direction arrow line view in (A) of FIG. 1). FIG. 2 is an exploded perspective view of the biosensor chip and the elastic body. FIG. 3 is an enlarged cross-sectional view of a main part in the embodiment of the present invention. FIG. 4 is a block diagram showing an embodiment of the biosensor device of the present invention. FIGS. 5A to 5C are a series of explanatory views showing a sample collecting operation using the biosensor device according to the present invention.

As shown in FIG. 1, a biosensor cartridge 10 according to an embodiment of the present invention includes a biosensor chip 11 having a sample collection port 13 for collecting a sample at a tip portion and a puncture instrument support 28. It is an integrated structure. The puncture device support 28 has the puncture device 12 fixed to one end thereof. The puncture device support 28 is integrated with the biosensor chip 11 so that the tip 12a of the puncture device 12 protrudes from the tip 11a side of the biosensor chip 11 and sandwiches the biosensor chip 11. Yes. An elastic body 20 is provided so as to surround the puncture device 12. Further, an inclined angle portion 11b having an angle of about 45 degrees is formed at the corner of the tip 11a of the biosensor chip 11, and the sample collection port 13 is opened at the inclined angle portion 11b. The opening of the sampling port 13 faces the puncture instrument 12 and faces it.
Further, as shown in FIGS. 2 and 3, the center line F of the hollow reaction part 15 constituting the opening of the sample collection port 13 and the axis C of the puncture device 12 intersect with each other at an angle of about 45 degrees, for example. Has been placed.

  In this way, the sample collection port 13 is provided at the inclined corner portion 11b formed at the corner of the tip 11a of the biosensor chip 11, so that the puncture instrument support 28 and the biosensor chip 11 are integrated. Sometimes it becomes easy to bring the sample collection port 13 and the puncture device 12 into a very close state.

  In the present embodiment, the puncture device support 28 is integrated so as to sandwich the biosensor chip 11 from the front and back. In the biosensor cartridge 10 configured as described above, the puncture instrument support 28 can be held so as to sandwich the relatively thin biosensor chip 11, so that the biosensor chip 11 can be easily and firmly held. It is easy to combine and integrate the members.

  The elastic body 20 is formed with a sealed semi-open space 23 that encloses a puncture opening formed in the subject M by the puncture instrument 12. Further, as shown in FIGS. 2 and 3, the elastic body 20 has a surface 25 in contact with the distal end 11 a of the biosensor chip 11 and the distal end 28 a of the puncture device support 28 joined by an adhesive or the like.

  In the present invention, the puncture device 12 is a generic term for a needle, a lancet needle, a cannula, and the like, and is preferably made of a biodegradable material.

  Note that the biosensor chip 11 includes two substrates 16a and 16b facing each other and a spacer layer 17 sandwiched between the two substrates 16a and 16b. Detection electrodes 18a and 18b are provided on the surface of at least one substrate 16a of the two substrates 16a and 16b on the spacer layer 17 side, at one end (the lower end in FIG. 1A). Are bent in a substantially oblique L shape in a direction opposite to each other to maintain a predetermined interval.

As shown in FIG. 3, the biosensor chip 11 includes two substrates 16 a and 16 b and a spacer layer 17 from the inclined angle portion 11 b of the tip 11 a to a portion where the two detection electrodes 18 a and 18 b face each other. Thus, the hollow reaction part 15 is formed.
One end (one end on the left side in FIG. 3) of the hollow reaction portion 15 is opened to the inclined angle portion 11b to constitute the sample collection port 13. That is, blood D (see FIG. 5C) collected by puncturing the subject M with the tip 12 a of the puncture device 12 is introduced into the hollow reaction part 15 from the sample collection port 13.

  The hollow reaction part 15 is formed of substrates 16a and 16b and detection electrodes 18a and 18b on both upper and lower surfaces, and the spacer layer 17 cut into a predetermined shape is used as a side wall at approximately 45 degrees (the center line F is inclined at approximately 45 degrees). ) And a rectangular space inclined. In the hollow reaction part 15, the detection electrodes 18a and 18b are appropriately exposed. For example, an enzyme and a mediator are fixed immediately above or in the vicinity of the detection electrodes 18a and 18b in the hollow reaction part 15, and glucose in the blood D is detected. A reagent 14 is provided which generates a current by reacting with. Therefore, the hollow reaction part 15 is a part where, for example, blood D, which is a sample collected from the sample collection port 13, biochemically reacts with the reagent 14.

  As the material of the substrates 16a and 16b and the spacer layer 17, an insulating material film is selected. As the insulating material, ceramics, glass, paper, biodegradable material (for example, polylactic acid microorganism-producing polyester), poly Examples thereof include thermoplastic resins such as vinyl chloride, polypropylene, polystyrene, polycarbonate, acrylic resin, polybutylene terephthalate and polyethylene terephthalate (PET), thermosetting resins such as epoxy resins, and plastic materials such as UV curable resins. A plastic material such as polyethylene terephthalate is preferable because of its mechanical strength, flexibility, and ease of chip fabrication and processing. Representative PET resins include Melinex and Tetron (trade names, manufactured by Teijin DuPont Films, Inc.), Lumirror (trade names, manufactured by Toray Industries, Inc.), and the like.

Examples of the reagent 14 include enzymes such as glucose oxidase (GOD), glucose dehydrogenase (GDH), cholesterol oxidase, uricase, and electron acceptors.
For example, in the case of a glucose biosensor chip that measures the amount of glucose in blood, this portion includes a glucose oxidase layer, a glucose oxidase-electron acceptor (mediator) mixture layer, a glucose oxidase-albumin mixture layer, or a glucose oxidase-electron. A receptor-albumin mixture layer or the like is formed. These layers may be formed using enzymes other than glucose oxidase, such as glucose dehydrogenase. Moreover, you may include a buffering agent, hydrophilic polymer, etc. as an additive in a chemical | medical agent.

As shown in FIGS. 2 and 3, the elastic body 20 attached to the tip 11a of the biosensor chip 11 and the tip 28a of the puncture device support 28 forms a sealed semi-open space 23 in the center, for example. The cylindrical thing which has the following through-holes 22 can be illustrated. The through hole 22 is configured to allow the puncture instrument 12 to be inserted and for the sample (blood D) to reach the sample collection port 13.
Therefore, in the sealed semi-open space 23, the through hole 2 is formed to be slightly larger (inner diameter W1) than the outer diameter of the puncture device 12, and the space on the specimen M side (lower side in the figure) is further from the through hole 22. Is configured to have a larger inner diameter (W2).

  When the opening of the sealed semi-open space 23 is configured to be large, the positional relationship required for collection of the sample D can be reliably maintained between the puncture port and the open space of the elastic body 20 at the time of puncturing. Moreover, the unexpected contact by the position shift etc. with the sample D and the elastic body 20 is avoided, and the trouble that the sample D oozes out to a contact site | part can be avoided.

  Further, the sealed half-open space 23 has a so-called through-hole 22 in which the so-called through-hole 22 has a raised portion 26 in which the wall surface around the through-hole 22 is raised. According to this configuration, the blood D that has flowed out into the sealed half-open space 23 first comes into contact with the raised portion 26 around the through hole 22 and is guided to the through hole 22. Therefore, since blood D first contacts a portion near the through hole 22 in the sealed semi-open space 23, introduction of the blood D into the sampling port 13 can be stabilized, and unexpected bleeding of the blood D to the outside is possible. It can prevent sticking out.

  Further, the thickness (t) of the elastic body 20 is configured to be able to reliably cover the tip 12 a of the puncture device 12.

The material of the elastic body 20 is not particularly limited as long as it has elasticity, but rubber such as silicone, urethane, acrylic rubber, rubber or sponge made of a single polymer or copolymerized polymer such as ethylene, styrene, Polyolefins such as polyethylene and polypropylene, polyesters such as polyethylene terephthalate and polybutylene terephthalate, fluororesins such as polytetrafluoroethylene and PFA which is a copolymer of perfluoroalkoxyethylene and polyfluoroethylene, and the like can be used.
The elastic body 20 may be solid or hollow.

  Further, at least the distal end surface 21 which is a surface in contact with the subject M of the elastic body 20 is made of a material such as adhesive silicone rubber or acrylic rubber, or the elastic body has the adhesive 24 or the adhesive 24. It is desirable to be coated with. The adhesive 24 is not particularly limited as long as the elasticity is not impaired. As a result, the adhesion between the elastic body 20 and the subject M can be improved, the displacement from the puncture position can be prevented, and the sealed semi-open space 23 can be reliably formed. Further, it is desirable that the inner peripheral surface of the through hole 22 is made of a hydrophilic material, or at least the inner peripheral surface is subjected to a hydrophilic treatment. Thereby, blood D to be collected can be easily passed, and even a small amount of blood D can be reliably collected.

It is desirable that the biosensor chip 11 and the puncture device support 28 and the elastic body 20 are securely fixed with an adhesive. At this time, when a gap is formed between the elastic body 20 and the biosensor chip 11 and the puncture device support 28, adhesion can be ensured by applying an adhesive so as to crush the gap. Moreover, it can prevent that the blood D extract | collected from the junction part by this adhesive agent leaks.
In the case where an adhesive is applied to the contact surface between the biosensor chip 11 and the puncture device support 28 and the elastic body 20, the adhesive is applied to a portion serving as a blood D flow path, the inside of the biosensor chip 11, and the like. Care must be taken not to protrude.

Next, the biosensor device according to the present invention will be described.
FIG. 4 shows a configuration of a biosensor device 30 using the biosensor cartridge 10 described above.
As shown in FIG. 4, the biosensor device 30 includes a biosensor cartridge 10 and a measuring instrument 31 that obtains information on blood D collected by connecting to the detection electrodes 18 a and 18 b of the biosensor cartridge 10. And a protective cap 36 for the biosensor cartridge.
Note that the configuration of the biosensor cartridge 10 is as described above, and the same reference numerals are given to the portions common to the biosensor cartridge 10 described above, and the description thereof is omitted here.

  The measuring device 31 includes a power supply 32, a control device 33, a terminal insertion unit 34, and a display unit 35, which are connected to each other. The rear end portion 11c of the biosensor chip 11 of the biosensor cartridge 10 is inserted and fixed to the terminal insertion portion 34, and the detection electrodes 18a and 18b exposed at the rear end portion 11c of the biosensor chip 11 are provided. It is designed to be electrically connected. The biosensor device 30 is small in size, for example, a handy type that a subject can hold with one hand.

Next, with reference to (A) to (C) of FIG. 5, a usage method will be described by taking as an example a case where a blood glucose level is measured using the biosensor device 30.
First, as shown in FIG. 4, the rear end portion 11 c of the biosensor chip 11 of the biosensor cartridge 10 is inserted into the terminal insertion portion 34 of the measuring instrument 31 to be fixed and electrically connected. The power source 32 of the biosensor device 30 is turned on and it is confirmed whether it is normally activated.

  Then, as shown in FIG. 5A, the elastic body that has the biosensor device 30 and presses the protective cap 36 against the subject to make the puncture site congested and is attached to the tip 11a of the biosensor cartridge 10. 20 is brought into contact with the blood collection site of the subject M. Here, since the pressure sensitive adhesive 24 is coated on the distal end surface 21 of the elastic body 20, misalignment is prevented in subsequent operations.

  Next, as shown in FIG. 5B, the biosensor cartridge 10 is pressed against the subject M. As a result, the elastic body 20 is crushed and the distal end 12a of the puncture device 12 protrudes from the distal end of the elastic body 20 to puncture the subject M.

Thereafter, the force for pressing the biosensor cartridge 10 is weakened. By weakening this pressing, the elastic body 20 returns to its original state (the state shown in FIG. 5A) by its restoring force, as shown in FIG. As a result, the tip 12a of the puncture device 12 is removed from the subject M. At this time, since the inside of the sealed semi-open space 23 including the puncture port is temporarily under negative pressure, the blood D easily flows out from the puncture port.
Further, since the inner peripheral surface of the through hole 22 is subjected to hydrophilic treatment, the blood D is guided to the sample collection port 13 along the inner peripheral surface of the through hole 22 and the puncture device 12 by the surface tension and capillary action. Collected. The collected blood D is introduced into the hollow reaction unit 15. At this time, since the sample collection port 13 is located in the sealed semi-open space 23 together with the puncture port formed by the puncture device 12, the blood D is collected easily and reliably without moving the biosensor cartridge 10. can do.
Thus, since a series of operations from the puncture operation to the collection of blood D can be performed with one operation, for example, it is easy to use even with the subject M with reduced visual acuity, and measurement with a small amount of blood is possible. The burden on the subject at the time can be reduced. Furthermore, since the sealed semi-open space 23 is blocked to some extent from the outside air, the blood D can be delayed in coagulation and can be collected easily.

When a predetermined amount of blood is collected as described above, the biosensor device 30 is separated from the subject M, and the measurement result is displayed on the display unit 35. The blood D introduced into the hollow reaction unit 15 reacts with the reagent 14, and the current value or charge value (charge amount) data measured by the detection electrodes 18 a and 18 b is sent to the control device 33. A calibration curve data table is stored in the control device 33, and the blood sugar level is calculated based on the measured current value (charge value). When the calculation is completed, the measurement result is displayed on the display unit 35. For example, the blood glucose level can be expressed as a numerical value. Finally, the biosensor cartridge 10 is removed from the measuring instrument 31. At this time, since the elastic body 20 has returned to its original height, the puncture instrument 12 does not protrude from the tip 11a of the biosensor chip 11. ing.
Thus, when it is in the state which does not protrude from the front-end | tip 11a of the biosensor chip | tip 11, a user can process the used biosensor cartridge 10 appropriately, without being damaged with the instrument 12 for puncture.

  For puncturing, in addition to puncturing the biosensor cartridge 10 against the subject M, for example, a drive mechanism may be used. Examples of the drive mechanism for puncturing the subject with the puncture device include a spring and a motor. By using these drive mechanisms, the time required for puncturing can be shortened, and pain during puncturing can be reduced.

  In consideration of the blood collection burden of the subject M, the volume of the hollow reaction part 15 is preferably 1 μL (microliter) or less, and particularly preferably 300 nL (nanoliter) or less. With such a minute hollow reaction part 15, even if the diameter of the puncture device 12 is small, a sufficient blood volume of the subject can be collected. Preferably, the diameter is 1000 μm or less.

  As described above, according to the biosensor cartridge 10 and the biosensor device 30 described above, when the biosensor chip 11 and the puncture device support 28 are pressed against the subject M, the elastic body 20 is compressed and the puncture device 12 is compressed. The distal end 12a of the projection protrudes and the subject M can be punctured. When the pressing force is weakened, the puncture device 12 is extracted from the subject M by the restoring force of the elastic body 20, and the blood D flows out from the puncture port. Since the blood D that has flowed out is guided to the sample collection port 13 formed in the inclined angle portion 11b of the biosensor chip 11 through the through hole 22 and the puncture device 12, a small amount of blood D is obtained from the micro puncture port. The sample can be collected effectively, and the pain of the subject M can be reduced. Moreover, since even a small amount of blood D can be easily collected and analyzed by the sample collection port 13, the burden on the subject M can be reduced.

  Further, by preventing the puncture device 12 from protruding from the distal end surface 21 of the elastic body 20 before use, the puncture device 12 and the user can be protected. Further, even when discarded after use, the puncture device 12 can be safely and properly disposed of by preventing it from protruding from the distal end surface 21 of the elastic body 20.

  Further, since puncture and sample collection can be performed in a series of one operation, it is not necessary to align the sample collection port 13 of the biosensor chip with the puncture port as in the prior art, and sample collection can be performed easily and reliably. It can be carried out. In addition, since the blood D information is transmitted to the measuring device 31 via the detection electrodes 18a and 18b, the measurement can be easily performed in a short time, and thus the burden on the subject M can be reduced.

The biosensor cartridge of the present invention is not limited to the embodiment described above, and appropriate modifications and improvements can be made.
For example, in the above-described embodiment, the inclination angle portion 11b of the biosensor chip 11 is inclined by approximately 45 degrees. However, the inclination angle is not limited to this and can be set to an appropriate angle.
Further, even if the angle is not provided, the sample collection port may be provided only at the corner of the tip.
Moreover, although the hollow reaction part 15 was also made into the rectangle inclined about 45 degree | times, the inclination angle and shape can be changed suitably. Moreover, in the said embodiment, although the case where the hollow reaction part 15 of the biosensor chip | tip 11 was provided in the spacer layer 17 pinched | interposed into both board | substrates 16a and 16b was illustrated, the biosensor cartridge of this invention is limited to this. Not what you want.

In the above-described embodiment, the case where the blood D is collected by the surface tension or capillary action has been described. However, for the application of the present invention, a device such as a pump that sucks up the blood D flowing out to the puncture port is used. it can. Further, it goes without saying that the detection electrodes 18a and 18b are not L-shaped but can be formed in a straight line shape or a shape as necessary.
Moreover, although the elastic body was provided in the said embodiment, the structure which does not provide this elastic body may be sufficient as this invention.

  As described above, in the biosensor cartridge according to the present invention, the sample collection port provided at one end of the biosensor chip is opened in the direction intersecting the axis of the tip of the puncture device, so that the sample collection port However, since the sample collection port can be made closer to the puncture port formed by the tip of the puncture device than before, even a small amount of sample can be collected easily by the sample collection port. Can be implemented. In addition, according to the present invention, a biosensor device capable of performing a reliable test can be provided.

(A) is explanatory drawing of the general | schematic longitudinal cross-section in embodiment of the biosensor cartridge which concerns on this invention. (B) is explanatory drawing of the general | schematic horizontal cross section in embodiment of the biosensor cartridge which concerns on this invention. It is the disassembled perspective view which decomposed | disassembled the elastic body and biosensor chip | tip in embodiment of the biosensor cartridge which concerns on this invention. It is sectional drawing which shows the principal part in embodiment of the biosensor cartridge which concerns on this invention. 1 is a schematic plan view showing an embodiment of a biosensor device according to the present invention. (A)-(C) are explanatory drawings which show the operation | movement which measures a blood glucose level using the biosensor apparatus concerning this invention. It is a disassembled perspective view which shows the conventional biosensor chip. (A) is a perspective view which shows the conventional biosensor chip. (B) is an exploded perspective view showing a conventional biosensor chip.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 Biosensor cartridge 11 Biosensor chip 11a Tip of biosensor chip 11b Inclined angle part 12 Puncture instrument 12a Tip of puncture instrument 13 Sample sampling port 15 Hollow reaction part 18a, 18b Electrode for detection 20 Elastic body 21 End surface (covered surface) The surface in contact with the specimen)
22 Through-hole 23 Sealed semi-open space (space)
24 Adhesive 25 Surface in contact with biosensor chip 26 Raised portion 28 Puncture instrument support 28a Tip of puncture instrument support 29 Open through hole (air passage)
30 Biosensor device 31 Measuring instrument D Blood (sample)
M subject

Claims (5)

A biosensor cartridge having a biosensor chip having a sample collection port for collecting a sample at the tip, and a puncture device fixed to the biosensor chip,
The sampling port opens in a direction intersecting the axis of the puncture device ;
An elastic body that includes a puncture port formed in a subject by the puncture device at the tip of the puncture device support that holds the biosensor chip and the puncture device to form a space necessary for sample collection biosensor cartridge, characterized in that is provided.   The biosensor cartridge according to claim 1, wherein the sampling port is provided at a corner of the tip of the biosensor chip. Before Ki穿 barbs instrument support biosensor cartridge according to claim 1 or 2 so as to sandwich the biosensor chip from its front and back, characterized in that it is integrated with said biosensor chip. The biosensor cartridge according to claim 1, wherein at least a tip surface of the elastic body has adhesiveness. A biosensor comprising: the biosensor cartridge according to any one of claims 1 to 4; and a measuring instrument that obtains information on a sample collected by connecting to a detection electrode of the biosensor cartridge. Sensor device.

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