JP3261956B2 - Analysis equipment - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an analyzer using immunochromatography, and more particularly to an improvement in which the completion of the measurement can be confirmed more reliably.

[0002]

2. Description of the Related Art Hitherto, as an analyzer using immunochromatography, for example, an analyzer which allows anyone to easily check for the presence or absence of pregnancy by detecting a specific hormone secreted in urine, etc. There is.

[0003] In such an analyzer, it is used that urine attached to one end of the chromatographic material moves through the chromatographic material, passes through a measurement area for detecting a specific hormone, and is completed. Conventionally, there is a method using a colloidal gold and a latex-labeled mouse antibody as a label in order to inform the subject.

[0004] This is because the anti-mouse antibody is immobilized on the chromatographic material downstream of the measurement area, and the labeling is observed at the portion where the anti-mouse antibody is immobilized by the antigen-antibody reaction. This allows the user to confirm the end of the measurement.

[0005]

However, in the conventional analyzer in which the completion of the measurement is confirmed by utilizing the above-mentioned antigen-antibody reaction, since the antigen and the antibody are proteins, the influence of temperature, humidity, etc. And there is a problem that the color density when colored is unstable. Antibodies were also very costly.

[0006]

SUMMARY OF THE INVENTION In order to solve this problem, the present invention provides an analyzer using immunochromatography, in which a water-insoluble colored material is immobilized on a chromatographic material, and the immobilized portion is immobilized. The upper surface is covered with a cover member that transmits light by adhering the solution.

Thus, when the measurement is completed after the solution has passed through the measurement area, light can be transmitted through the cover member and the color of the water-insoluble colored material can be confirmed. It is possible to inform the user with a sign of a certain density without being influenced.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION According to the first aspect of the present invention, a solution applied to one end of a chromatography material passes through a measurement area for detecting an analyte contained in the solution. In order to detect the end of the measurement, a water-insoluble colored material is immobilized on the chromatography material at a position downstream of the measurement area, and the water-insoluble colored material is further immobilized. The analytical device, wherein the upper surface of the chromatographic material obtained is covered with a cover member that transmits light when the solution adheres, in a predetermined location of the chromatographic material. The water-insoluble colored material immobilized in the deformed shape cannot be confirmed until the solution arrives because it is covered by the cover member, but the impermeable member becomes transparent as the solution arrives. By changing the sex, so that color can be confirmed clearly.

According to a second aspect of the present invention, there is provided the analyzer according to the first aspect, wherein the water-insoluble colored material is fixed to the cover member. The color of the water-soluble colored material can be more clearly confirmed.

According to a third aspect of the present invention, the solution applied to one end of the chromatography material passes through a measurement area for detecting an analyte contained in the solution, and the measurement is performed. In order to be able to detect that it has ended,
At a position on the downstream side of the measurement area, a water-insoluble colored material is immobilized on the chromatography material, and the water-insoluble colored material is immobilized on the upper surface of the immobilized chromatography material, and is water-soluble and transmits light. In the analyzer, wherein the immobilized colored material is configured to be exposed by elution of the substance, and the immobilized colored material is immobilized in a predetermined shape at a predetermined location of the chromatography material. Until the solution arrives, the water-insoluble colored material cannot be confirmed because it is covered with the water-soluble impermeable material.However, the water-soluble impermeable material elutes with the arrival of the solution, and the color of the water-insoluble colored material changes. You will be able to see clearly.

(Embodiment 1) An embodiment of the invention described in claim 1 of the present invention will be described below with reference to FIG. In the analyzer shown in FIG. 1, reference numeral 1 denotes a chromatography material, which is made of glass fiber filter paper. 2 indicates an antigen measurement region using an antigen-antibody reaction on the chromatography material,
The antibody immobilized on the glass fiber filter paper and the gold colloid-labeled antibody are subjected to a sandwich reaction with an antibody having another epitope to measure the antigen.

Reference numeral 4 denotes a water-insoluble colored material immobilized on a chromatography material, which uses oil-based red ink. Reference numeral 3 denotes a cover member provided so as to cover the water-insoluble colored material 4, and is made of a material that transmits light by adhering a solution, for example, nitrocellulose.

When the solution is added to one end of the chromatography material 1, the solution moves through the chromatography material 1, and the analyte performs an antigen-antibody reaction in the measurement area 2. Thereafter, the solution further proceeds on the chromatography material 1 to the downstream side, and reaches the portion where the water-insoluble colored material 4 is immobilized. At the same time, the adhesion of the solution causes the cover member 3
Changes to transmit light, so that the water-insoluble colored material 4
Can be recognized from the outside, and the end of the measurement can be confirmed.

The member which becomes permeable by the adhesion of the solution is
As long as the material changes in permeability due to the adhesion of the solution, it can be made of any material such as a hygroscopic material, a porous material, a fiber material, and a moisture-impermeable material.

(Embodiment 2) An embodiment of the invention described in claim 2 of the present invention will be described below with reference to FIG. In the analyzer of FIG. 2, reference numeral 5 denotes a chromatography material, which is made of glass fiber filter paper. Reference numeral 6 denotes an antigen measurement region using an antigen-antibody reaction. An antibody immobilized on glass fiber filter paper and an antibody labeled with colloidal gold are subjected to a sandwich reaction with an antibody having another epitope to measure the antigen. . In addition, glass fiber filter paper was used for the cover member 7 that was provided on the chromatography material 5 downstream of the measurement area 6 and that became light-permeable due to the adhesion of the solution. A water-insoluble colored material 8 is fixed to the cover member 7. As the water-insoluble colored material 8, oil-based red ink was used.

Before the solution arrives, the water-insoluble colored member 8 cannot be seen from above the cover member 7. When the solution is added, the solution proceeds with the chromatography material, and the analyte performs an antigen-antibody reaction in the reaction region 6. Thereafter, the solution further proceeds through the chromatography material 5 and reaches the portion where the water-insoluble colored material 8 is immobilized. At the same time, since the cover member 7 transmits light due to the adhesion of the solution, the water-insoluble colored material 8 can be recognized from above, and the end of the measurement can be confirmed.

In the above description, a member made of glass fiber filter paper is described as a member that becomes permeable by the adhesion of a solution. Any material such as a porous material, a fiber material, and a moisture-impermeable material can be used.

[0018] (Embodiment 3) Hereinafter, embodiments of the invention described in claim 3 of the present invention will be described with reference to FIG. In the analyzer of FIG. 3 , reference numeral 12 denotes a chromatography material, which is made of glass fiber filter paper. Reference numeral 13 denotes an antigen measurement region using an antigen-antibody reaction, and the antigen is measured by a sandwich reaction between an antibody immobilized on glass fiber filter paper and an antibody having an epitope different from the gold colloid-labeled antibody. The water-insoluble colored material 15 fixed downstream of the measurement region 13 uses oil-based red ink.
Sodium chloride is provided as the water-soluble impermeable material 14 so as to cover the water-insoluble colored material 15.

When the solution is added, the chromatographic material 12 advances, and the analyte performs an antigen-antibody reaction in the reaction region 13. Thereafter, the solution further proceeds on the chromatography material, and reaches the portion where the water-insoluble colored material 15 is immobilized. At the same time, the water-soluble impermeable material 14 elutes and the water-insoluble colored material 15 is exposed, so that it can be recognized from the outside and the end of the measurement can be confirmed. Here, sodium chloride has been described as an example of the water-soluble impermeable material,
In addition, the present invention can be similarly implemented by using a water-soluble impermeable material such as inorganic salts and organic salts.

In the above description, an example was described in which the water-insoluble colored material was constituted by oil-based red ink.
The same applies to water-insoluble colored tapes, water-insoluble colored inks, and water-insoluble dyes.

Further, in the above description, an example was described in which the glass fiber filter paper was used as the chromatography material.
The present invention can be similarly applied to a porous or fibrous material such as cellulose, a cellulose derivative, and filter paper.

[0022]

As described above, according to the present invention, it is possible to obtain an advantageous effect that the end of measurement in immunochromatography can be easily confirmed with high accuracy, low cost, and simply by using a water-insoluble colored material.

[Brief description of the drawings]

FIG. 1 is an exploded perspective view showing an analyzer according to one embodiment of the present invention.

FIG. 2 is an exploded perspective view showing an analyzer according to one embodiment of the present invention.

FIG. 3 1 shows an analyzer according to an embodiment of the present invention.
Perspective view

[Explanation of symbols]

 1,5,12 Chromatography material 2,6,13 Measurement area 3,7 Cover member 4,8,15 Non-water-soluble colored material 15 Non-water-soluble colored material

──────────────────────────────────────────────────続 き Continued on the front page (56) References Registered utility model 3025595 (JP, U) (58) Fields investigated (Int. Cl. ⁷ , DB name) G01N 33/543 G01N 30/95

Claims (3)

(57) [Claims] (1) A solution applied to one end of a chromatography material passes through a measurement area for detecting an analyte contained in the solution, and is used to detect that the measurement has been completed. At a position downstream of the measurement area, a water-insoluble colored material is immobilized on the chromatography material, and further, the upper surface of the chromatography material on which the water-insoluble colored material is immobilized, when the solution adheres. An analyzer characterized by being covered with a cover member that transmits light. 2. The analyzer according to claim 1, wherein the water-insoluble colored material is fixed to the cover member. 3. A method in which a solution applied to one end of a chromatography material passes through a measurement area for detecting an analyte contained in the solution, and the completion of the measurement is made detectable. At a position downstream of the measurement region, a water-insoluble colored material is immobilized on the chromatography material, and the upper surface of the chromatography material on which the water-insoluble colored material is immobilized is water-soluble and emits light. An analyzer that is covered with a substance that does not pass therethrough, and is configured such that the immobilized colored material is exposed by elution of the substance.