Nothing Special   »   [go: up one dir, main page]

ES2152209T3 - Proteasa de subtilisina mutada. - Google Patents

Proteasa de subtilisina mutada.

Info

Publication number
ES2152209T3
ES2152209T3 ES90910604T ES90910604T ES2152209T3 ES 2152209 T3 ES2152209 T3 ES 2152209T3 ES 90910604 T ES90910604 T ES 90910604T ES 90910604 T ES90910604 T ES 90910604T ES 2152209 T3 ES2152209 T3 ES 2152209T3
Authority
ES
Spain
Prior art keywords
enzymes
muted
subtilisin protease
genes
mutating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90910604T
Other languages
English (en)
Inventor
Sven Branner
Sven Hastrup
Ole Hvilsted Olsen
Leif Norskov-Lauritsen
Merete Simonsen
Dorrit Aaslyng
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8119873&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2152209(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2152209T3 publication Critical patent/ES2152209T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE DESCRIBEN ENCIMAS PRODUCIDAS AL MUTAR LOS GENES DE UN NUMERO DE PROTEASAS DE SUBTILISINA Y EXPRESAR LOS GENES MUTADOS EN UN RECEPTOR APROPIADO. LAS ENCIMAS MUTADAS TIENEN UNA CARGA ELECTROSTATICA DE RED CAMBIADA COMPARANDOLA CON LA PROTEASA PADRE DEL MISMO PH. LAS ENCIMAS EXHIBEN UNA REALIZACION DE LAVADO MEJORADA EN COMPARACION CON SUS ENCIMAS PADRES DE TIPO VIRGEN. LAS ENCIMAS SON APROPIADAS PARA UTILIZARLAS EN COMPOSICIONES DETERGENTES.
ES90910604T 1989-06-26 1990-06-26 Proteasa de subtilisina mutada. Expired - Lifetime ES2152209T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK316989A DK316989D0 (da) 1989-06-26 1989-06-26 Enzymer

Publications (1)

Publication Number Publication Date
ES2152209T3 true ES2152209T3 (es) 2001-02-01

Family

ID=8119873

Family Applications (2)

Application Number Title Priority Date Filing Date
ES90910604T Expired - Lifetime ES2152209T3 (es) 1989-06-26 1990-06-26 Proteasa de subtilisina mutada.
ES99102452T Expired - Lifetime ES2242314T3 (es) 1989-06-26 1990-06-26 Subtisilina proteasa mutada.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES99102452T Expired - Lifetime ES2242314T3 (es) 1989-06-26 1990-06-26 Subtisilina proteasa mutada.

Country Status (12)

Country Link
EP (5) EP1555314B1 (es)
JP (3) JP3343115B2 (es)
KR (1) KR100258459B1 (es)
AT (5) ATE362525T1 (es)
AU (1) AU5956690A (es)
BR (1) BR9007477A (es)
CA (1) CA2062732C (es)
DE (6) DE69034241T2 (es)
DK (3) DK316989D0 (es)
ES (2) ES2152209T3 (es)
FI (3) FI102907B1 (es)
WO (1) WO1991000345A1 (es)

Families Citing this family (169)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
DE4023458A1 (de) * 1989-08-31 1991-03-07 Kali Chemie Ag Neue hochalkalische proteasen
US5352603A (en) * 1989-08-31 1994-10-04 Kali-Chemie Ag Highly alkaline proteases
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
AU9052891A (en) * 1990-12-05 1992-07-08 Novo Nordisk A/S Proteins with changed epitopes and methods for the production thereof
ES2110488T3 (es) * 1990-12-21 1998-02-16 Novo Nordisk As Mutantes enzimaticos con un bajo grado de variacion de la carga molecular en un intervalo de ph.
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DE4106525A1 (de) * 1991-03-01 1992-09-10 Solvay Enzymes Gmbh & Co Kg Verfahren zur verbesserung der stabilitaet von hochalkalischen proteasen
JP3471797B2 (ja) * 1991-05-01 2003-12-02 ノボザイムス アクティーゼルスカブ 安定化酵素及び洗剤
ES2090481T3 (es) * 1991-05-01 1996-10-16 Unilever Nv Composiciones detergentes que contienen enzimas estabilizadas.
US5275945A (en) * 1991-10-08 1994-01-04 Vista Chemical Company Alkaline proteases stable in heavy-duty detergent liquids
HUT66846A (en) * 1991-10-16 1995-01-30 Unilever Nv Aqueous enzymatic detergent compositions
AU682314B2 (en) * 1992-07-17 1997-10-02 Genencor International, Inc. High alkaline serine proteases
KR100303619B1 (ko) * 1992-10-06 2001-11-22 피아 스타르 셀룰라제변이체
DE4304161A1 (de) * 1993-02-12 1994-08-25 Solvay Enzymes Gmbh & Co Kg Verbesserte hochalkalische Proteasen
DK39093D0 (da) * 1993-04-01 1993-04-01 Novo Nordisk As Enzym
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
CN1134726A (zh) * 1993-10-04 1996-10-30 诺沃挪第克公司 一种包含修饰酶的酶制剂
CA2173106C (en) * 1993-10-14 2000-02-15 Michael Eugene Burns Bleaching compositions comprising protease enzymes
MA23346A1 (fr) 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
CA2173105C (en) * 1993-10-14 2003-05-27 Andre Baeck Protease-containing cleaning compositions
ES2364776T3 (es) * 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
ATE390487T1 (de) 1994-02-24 2008-04-15 Henkel Kgaa Verbesserte enzyme und diese enthaltene detergentien
US6110884A (en) * 1994-03-29 2000-08-29 Novo Nordisk A/S Protease variants
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
IL117352A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Thermitase variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
DE69637907D1 (de) * 1995-05-05 2009-06-04 Novozymes As Protease-varianten und verbindungen
US5837517A (en) 1995-05-05 1998-11-17 Novo Nordisk A/S Protease variants and compositions
US6682924B1 (en) 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
DE19530816A1 (de) 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
KR100591553B1 (ko) 1996-11-04 2006-06-19 노보자임스 에이/에스 섭틸라제 변종과 조성물
CA2270593C (en) * 1996-11-04 2005-06-07 Novo Nordisk A/S Subtilase variants and compositions
EP1009815B1 (en) * 1997-08-29 2008-01-30 Novozymes A/S Protease variants and compositions
CN1272137A (zh) 1997-08-29 2000-11-01 诺沃挪第克公司 蛋白酶变体及组合物
ES2367505T3 (es) 1997-10-23 2011-11-04 Danisco Us Inc. Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes.
AR016969A1 (es) * 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
ATE298791T1 (de) 1997-11-21 2005-07-15 Novozymes As Protease-varianten und zusammensetzungen
CA2313950A1 (en) † 1997-12-24 1999-07-08 James T. Kellis, Jr. An improved method of assaying for a preferred enzyme and /or preferred detergent composition
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
WO2000024924A2 (en) * 1998-10-23 2000-05-04 The Procter & Gamble Company Methods for screening protease variants for use in detergent compositions
DE69936933T2 (de) * 1998-12-18 2008-05-15 Novozymes A/S SUBTILASE ENZYME DER I-S1 und I-S2 UNTERGRUPPEN MIT EINEM ZUSÄTZLICHEN AMINOSÄUREREST IN EINER AKTIVEN SCHLEIFENREGION
DE69943346D1 (de) 1998-12-18 2011-05-19 Novozymes As Subtilase Enzyme der I-S1- und I-S2-Untergruppen mit einem zusätzlichen Aminosäurerest in einer aktiven Schleifenregion
KR100660799B1 (ko) * 1998-12-18 2006-12-26 노보자임스 에이/에스 활성부위 루프 영역에 추가적 아미노산 잔기를 가지는서브그룹 ｉ-ｓ１과 ｉ-ｓ２의 서브틸라제 효소
EP1183342B2 (en) † 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
AU4392800A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 126 and 127
ATE408677T1 (de) † 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 97 und 98
EP1183343B2 (en) 1999-05-20 2013-11-27 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 125 and 126
WO2000071689A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 127 and 128
EP1183337B1 (en) 1999-05-20 2008-09-17 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 132 and 133
EP1183339B2 (en) 1999-05-20 2013-03-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 129 and 130
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
CN100591763C (zh) 2000-08-21 2010-02-24 诺维信公司 枯草杆菌酶
WO2002031133A1 (en) 2000-10-13 2002-04-18 Novozymes A/S Subtilase variants
FI111728B (fi) * 2000-10-13 2003-09-15 Valtion Teknillinen Menetelmä proteiinin rakenteen muuttamiseksi
US6673580B2 (en) 2000-10-27 2004-01-06 Genentech, Inc. Identification and modification of immunodominant epitopes in polypeptides
US6803222B2 (en) * 2000-11-22 2004-10-12 Kao Corporation Alkaline proteases
GB0102929D0 (en) * 2001-02-06 2001-03-21 Inpharmatica Ltd Novel proteins
US20050101012A1 (en) 2001-03-12 2005-05-12 Gerold Schuler CD4+CD25+ regulatory T cells from human blood
DK200101090A (da) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
EP1321513A1 (en) * 2001-12-21 2003-06-25 Direvo Biotech AG Subtilisin variants with improved characteristics
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
WO2003062381A2 (en) * 2002-01-16 2003-07-31 Genencor International, Inc. Multiply-substituted protease variants
CN100386434C (zh) 2002-03-27 2008-05-07 诺和酶股份有限公司 具有丝状包衣的颗粒
US8222033B2 (en) 2002-08-12 2012-07-17 Argos Therapeutics, Inc. CD4+CD25− T cells and Tr1-like regulatory T cells
TWI319007B (en) 2002-11-06 2010-01-01 Novozymes As Subtilase variants
US7888093B2 (en) 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
ATE461276T1 (de) 2003-01-27 2010-04-15 Novozymes As Enzymstabilisierung
ATE368105T1 (de) 2003-02-07 2007-08-15 Novozymes As Proteasen
EP1606381B1 (en) 2003-03-18 2009-05-27 Novozymes A/S Coated enzyme granules
EP1623029B1 (en) 2003-05-07 2008-09-03 Novozymes A/S Variant subtilisin enzymes (subtilases)
EP2284259A3 (en) 2003-10-10 2011-06-15 Novozymes A/S Protease variants
EP1678296B1 (en) 2003-10-23 2011-07-13 Novozymes A/S Protease with improved stability in detergents
PT1691814E (pt) 2003-12-01 2012-10-16 Cambridge Entpr Ltd Agentes anti-inflamatórios
CN1942584B (zh) 2004-02-13 2011-07-27 诺维信公司 蛋白酶变体
AU2005254611B2 (en) * 2004-06-21 2010-09-23 Novozymes A/S Proteases
CN103182387B (zh) 2004-09-10 2016-01-06 诺维信北美公司 防止、去除、减少或破坏生物膜的方法
DE10180655T8 (de) 2004-09-27 2013-04-25 Novozymes A/S Phytasegranulate in der Tierernährung
EP2385112B1 (en) 2005-07-08 2016-11-30 Novozymes A/S Subtilase variants
WO2007036235A1 (en) 2005-09-30 2007-04-05 Novozymes A/S Immobilization of enzymes
EP1994130A1 (en) 2006-03-02 2008-11-26 Novozymes A/S High capacity encapsulation process
EP2004789B1 (en) 2006-03-31 2012-08-29 Novozymes A/S A stabilized liquid enzyme composition
BRPI0709978A2 (pt) 2006-04-14 2011-08-02 Genencor Int tratamento em etapa única de produtos têxteis
US20090215663A1 (en) 2006-04-20 2009-08-27 Novozymes A/S Savinase variants having an improved wash performance on egg stains
ES2570334T3 (es) * 2006-06-02 2016-05-17 Harvard College Remodelado superficial de proteínas
JP5396270B2 (ja) * 2006-06-02 2014-01-22 プレジデント アンド フェロウズ オブ ハーバード カレッジ タンパク質表面のリモデリング
KR20150115023A (ko) * 2006-07-18 2015-10-13 다니스코 유에스 인크. 광범위한 온도에 걸쳐 활성인 프로테아제 변이형
CN101500430B (zh) 2006-08-07 2014-02-19 诺维信公司 用于动物饲料的酶团粒
US20080031998A1 (en) 2006-08-07 2008-02-07 Novozymes A/S Enzyme Granules for Animal Feed
CN101522878B (zh) * 2006-10-06 2012-11-14 诺维信公司 洗涤剂组合物及其中酶组合的使用
JP2008212084A (ja) 2007-03-06 2008-09-18 Kao Corp アルカリプロテアーゼ
DK2147098T3 (da) 2007-04-30 2013-10-14 Danisco Us Inc Anvendelse af proteinhydrolysater til stabilisering af metalloprotease-detergent-formuleringer
RU2520808C2 (ru) * 2007-06-06 2014-06-27 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН Способ идентификации улучшенных вариантов белка
CA2690032C (en) * 2007-06-06 2018-05-15 Danisco Us Inc. Methods for improving protein properties
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
EP2262885B1 (en) 2008-03-28 2013-05-15 Novozymes A/S Triggered release system
EP2297314B1 (en) * 2008-06-06 2015-04-15 Danisco US Inc. Compositions and methods comprising variant microbial proteases
BRPI0915728A2 (pt) 2008-07-07 2015-10-27 Basf Se composição enzimática, processo para preparar a composição, uso da composição enzimática, e, composição detergente.
WO2010028941A1 (en) 2008-09-12 2010-03-18 Unilever Plc Dispenser and pretreater for viscous liquids
JP5727378B2 (ja) * 2008-11-11 2015-06-03 ダニスコ・ユーエス・インク セリンプロテアーゼ変異体を含む組成物及び方法
MX2011004802A (es) 2008-11-11 2011-06-16 Danisco Inc Bacilos de subtilisina que comprenden una o mas mutaciones combinables.
EP2202290A1 (en) 2008-12-23 2010-06-30 Unilever PLC A flowable laundry composition and packaging therefor
JP2012525146A (ja) 2009-04-28 2012-10-22 プレジデント アンド フェロウズ オブ ハーバード カレッジ 細胞透過のための過剰に荷電されたタンパク質
FI121712B (fi) 2009-04-30 2011-03-15 Ab Enzymes Oy Uusi sieniperäinen proteaasi ja sen käyttö
FI121711B (fi) 2009-04-30 2011-03-15 Ab Enzymes Oy Sieniperäinen seriiniproteaasi ja sen käyttö
FI121851B (fi) 2009-07-08 2011-05-13 Ab Enzymes Oy Sieniperäinen proteaasi ja sen käyttö
US20130260438A1 (en) * 2010-05-06 2013-10-03 Danisco Us Inc. Compositions and methods comprising serine protease variants (as amended)
DE102010028951A1 (de) 2010-05-12 2011-11-17 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Lipase
BR112012033774B1 (pt) 2010-07-01 2021-08-17 Novozymes A/S Método para branqueamento de polpa, composição de branqueamento, e, uso da composição
US20130224757A1 (en) 2010-08-19 2013-08-29 Novozymes A/S Induced sporulation screening method
FI123942B (fi) 2010-10-29 2013-12-31 Ab Enzymes Oy Sieniperäisen seriiniproteaasin variantteja
DE102011005354A1 (de) 2011-03-10 2012-09-13 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasevarianten
FI123425B (fi) 2011-03-31 2013-04-30 Ab Enzymes Oy Proteaasientsyymi ja sen käytöt
GB201106409D0 (en) 2011-04-15 2011-06-01 Revolymer Ltd Novel composite
GB201106391D0 (en) 2011-04-15 2011-06-01 Reckitt & Colman Overseas Novel composite
GB201106408D0 (en) 2011-04-15 2011-06-01 Revolymer Ltd Novel composite
BR112013027333A2 (pt) 2011-04-28 2016-11-29 Novozymes As célula hospedeira microbiana transgênica, construto de ácido nucleico, vetor de expressão, polipeptídeo isolado tendo atividade de endoglucanase, polinucleotídeo isolado, métodos para produzir um polipeptídeo com atividade de endoglucanase, para produzir um mutante de uma célula precursora, para inibir a expressão de um polipeptídeo com atividade de endoglucanase em uma célula, para produzir uma proteína, para degradar ou converter um material celulósico, para produzir um produto de fermentação, e para fermentar um material celulósico.
RU2663114C2 (ru) * 2011-05-05 2018-08-01 Дзе Проктер Энд Гэмбл Компани Способы и композиции, содержащие варианты сериновой протеазы
US9856466B2 (en) 2011-05-05 2018-01-02 Danisco Us Inc. Compositions and methods comprising serine protease variants
WO2013024143A1 (en) 2011-08-18 2013-02-21 Unilever Plc Enzyme system
BR112014005290B1 (pt) 2011-09-09 2021-11-09 Novozymes A/S Método para melhoramento da resistência do papel
AU2013258083A1 (en) 2012-05-10 2014-11-20 Unilever Plc Care enzyme system
WO2014067933A1 (en) 2012-10-31 2014-05-08 C-Lecta Gmbh Bioactive carrier preparation for enhanced safety in care products and food
EP2740840A1 (en) 2012-12-07 2014-06-11 Novozymes A/S Improving drainage of paper pulp
EP3140399B1 (en) 2014-07-04 2018-03-28 Novozymes A/S Subtilase variants and polynucleotides encoding same
US10626388B2 (en) 2014-07-04 2020-04-21 Novozymes A/S Subtilase variants and polynucleotides encoding same
WO2016007309A1 (en) 2014-07-07 2016-01-14 Novozymes A/S Use of prehydrolysate liquor in engineered wood
EP3227444B1 (en) 2014-12-04 2020-02-12 Novozymes A/S Subtilase variants and polynucleotides encoding same
EP3608403A3 (en) * 2014-12-15 2020-03-25 Henkel AG & Co. KGaA Detergent composition comprising subtilase variants
WO2016097350A1 (en) 2014-12-19 2016-06-23 Novozymes A/S Protease variants and polynucleotides encoding same
US10590367B2 (en) 2014-12-19 2020-03-17 Novozymes A/S Protease variants and polynucleotides encoding same
WO2016097357A1 (en) 2014-12-19 2016-06-23 Novozymes A/S Protease variants and polynucleotides encoding same
EP3234093B1 (en) 2014-12-19 2020-05-27 Novozymes A/S Protease variants and polynucleotides encoding same
CN107567489A (zh) 2015-04-10 2018-01-09 诺维信公司 衣物洗涤方法，dna酶和洗涤剂组合物的用途
US20180105772A1 (en) 2015-04-10 2018-04-19 Novozymes A/S Detergent composition
WO2017050441A1 (en) 2015-09-24 2017-03-30 Unilever N.V. Substrate washing device
CN108603187A (zh) 2015-11-24 2018-09-28 诺维信公司 具有蛋白酶活性的多肽以及编码它们的多核苷酸
WO2017207762A1 (en) 2016-06-03 2017-12-07 Novozymes A/S Subtilase variants and polynucleotides encoding same
WO2018224379A1 (en) 2017-06-09 2018-12-13 Unilever Plc Laundry liquid dispensing system
WO2020099490A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising enzymes
US20220015394A1 (en) 2018-12-05 2022-01-20 Novozymes A/S Use of An Enzyme Granule
WO2021018751A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Enzymatic treatment of paper pulp
CN112852788B (zh) * 2019-11-26 2022-10-11 江南大学 一种碱性底物选择性提高的枯草蛋白酶e突变体及其应用
US20230024021A1 (en) 2019-12-05 2023-01-26 Conopco, Inc., D/B/A Unilever Biodegradable package containing water-soluble capsules
EP4158097A1 (en) 2020-05-29 2023-04-05 Novozymes A/S Method for controlling slime in a pulp or paper making process
KR20230057391A (ko) 2020-08-24 2023-04-28 노보자임스 에이/에스 프럭타나제를 포함하는 구강 관리 조성물
CN116096731A (zh) * 2020-09-22 2023-05-09 巴斯夫欧洲公司 蛋白酶和蛋白酶抑制剂与第二酶的改进的组合
GB2607442A (en) 2021-05-14 2022-12-07 Unilever Global Ip Ltd Package containing water-soluble capsules
EP4337553A1 (en) 2021-05-14 2024-03-20 Unilever IP Holdings B.V. Package containing water-soluble capsules
WO2022238449A1 (en) 2021-05-14 2022-11-17 Unilever Ip Holdings B.V. Package containing water-soluble capsules
FR3122869A1 (fr) 2021-05-14 2022-11-18 Unilever Ip Holdings B.V. Conditionnement contenant des capsules solubles dans l’eau
EP4304945A1 (en) 2021-05-14 2024-01-17 Unilever IP Holdings B.V. Package containing water-soluble capsules
EP4337552A1 (en) 2021-05-14 2024-03-20 Unilever IP Holdings B.V. Package containing water-soluble capsules
FR3123330A1 (fr) 2021-05-25 2022-12-02 Unilever Ip Holdings B.V. Conditionnement contenant des capsules solubles dans l'eau
US20240279875A1 (en) 2021-06-16 2024-08-22 Novozymes A/S Method for controlling slime in a pulp or paper making process
WO2023078594A1 (en) 2021-11-05 2023-05-11 Unilever Ip Holdings B.V. Package containing water-soluble capsules
WO2023104646A1 (en) 2021-12-07 2023-06-15 Unilever Ip Holdings B.V. Package containing water-soluble capsules
DE202022102650U1 (de) 2022-05-13 2022-06-13 Unilever Global Ip Limited Wasserlösliche Kapseln enthaltende Packung
DE202022102651U1 (de) 2022-05-13 2022-05-30 Unilever Global Ip Limited Wasserlösliche Kapseln enthaltende Packung
WO2024121070A1 (en) * 2022-12-05 2024-06-13 Novozymes A/S Protease variants and polynucleotides encoding same

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1234445A (es) 1967-10-03 1971-06-03
US3929887A (en) 1970-05-18 1975-12-30 Endo Lab Alkylenepoly(aralkylamines) and the salts thereof
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
WO1987005050A1 (en) * 1986-02-12 1987-08-27 Genex Corporation Mutagenesis and screening method and product
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer

Also Published As

Publication number Publication date
JP3532902B2 (ja) 2004-05-31
EP1553174A2 (en) 2005-07-13
FI20031209A (fi) 2003-08-28
FI916050A0 (fi) 1991-12-20
EP0945502B1 (en) 2005-04-20
EP1553174A3 (en) 2006-02-22
DE69033644D1 (de) 2000-11-09
DE69034243D1 (de) 2007-06-28
EP1553173B1 (en) 2007-05-16
DE69034243T2 (de) 2008-01-24
CA2062732A1 (en) 1990-12-27
CA2062732C (en) 2000-05-23
JP2002272488A (ja) 2002-09-24
FI102907B (fi) 1999-03-15
DK0479870T3 (da) 2001-04-17
EP1553174B1 (en) 2007-05-09
DE69034187D1 (de) 2005-05-25
EP0479870B1 (en) 2000-10-04
ATE196774T1 (de) 2000-10-15
EP1555314B1 (en) 2009-09-30
ES2242314T3 (es) 2005-11-01
FI112500B (fi) 2003-12-15
KR100258459B1 (en) 2000-06-01
DE945502T1 (de) 2002-10-17
BR9007477A (pt) 1992-06-02
KR920702719A (ko) 1992-10-06
DE69033644T2 (de) 2001-05-10
DE69034187T2 (de) 2006-03-09
DK0945502T3 (da) 2005-08-22
EP0479870A1 (en) 1992-04-15
DE69034241T2 (de) 2008-01-10
AU5956690A (en) 1991-01-17
EP1553173A3 (en) 2006-01-25
DE69034241D1 (de) 2007-06-21
ATE293687T1 (de) 2005-05-15
EP0945502A1 (en) 1999-09-29
FI990023A (fi) 1999-01-08
EP1555314A3 (en) 2006-01-25
FI102907B1 (fi) 1999-03-15
DE69034266D1 (de) 2009-11-12
JP3343115B2 (ja) 2002-11-11
ATE361974T1 (de) 2007-06-15
WO1991000345A1 (en) 1991-01-10
JP2002315591A (ja) 2002-10-29
EP1555314A2 (en) 2005-07-20
ATE444357T1 (de) 2009-10-15
FI990023A0 (fi) 1999-01-08
JPH04505856A (ja) 1992-10-15
ATE362525T1 (de) 2007-06-15
DK316989D0 (da) 1989-06-26
EP1553173A2 (en) 2005-07-13

Similar Documents

Publication Publication Date Title
ES2152209T3 (es) Proteasa de subtilisina mutada.
BR9712473A (pt) Variantes de subtilase e composições
DE69839076D1 (de) Proteasevarianten und zusammensetzungen
DE69739020D1 (de) Subtilase varianten und verbindungen
DK305984D0 (da) Prokaryotiske carbonylhydrolaser, deres fremstilling og manipulering ved rekombinant teknik og deres anvendelse i vaskemidler
EP1160327A3 (en) Novel proteolytic enzymes and their use in detergents
DK1009795T3 (da) Detergentkompositioner indeholdende en mannanase og en protease
DE68924444D1 (de) Enzyme enthaltendes Geschirrwaschmittel.
ES2061717T3 (es) Composiciones de limpieza conteniendo proteasas producidas por vibrio.
EP0199404A3 (en) Liquid detergents containing anionic surfactant, builder and proteolytic enzyme
DK0629692T3 (da) Flydende enzymtilberedninger
BR9811412A (pt) Variante de enzima subtilase tendo melhorado desempenho de lavagem em detergentes, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, processos para produzir uma variante e para identificar uma variante de protease, composição, e, uso de uma variante de subtilase
BR9811396A (pt) Variante de enzima subtilase tendo melhorado desempenho de lavagem em detergentes, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, processos para produzir uma variante e para identificar uma variante de protease, composição, e, uso de uma variante de subtilase

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 479870

Country of ref document: ES