Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
  • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
  • C07D487/04—Ortho-condensed systems
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
  • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/10—Antimycotics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/12—Antivirals
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
  • A61P35/02—Antineoplastic agents specific for leukemia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P37/00—Drugs for immunological or allergic disorders
  • A61P37/02—Immunomodulators
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
  • C07D401/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D473/00—Heterocyclic compounds containing purine ring systems
  • C07D473/26—Heterocyclic compounds containing purine ring systems with an oxygen, sulphur, or nitrogen atom directly attached in position 2 or 6, but not in both
  • C07D473/28—Oxygen atom
  • C07D473/30—Oxygen atom attached in position 6, e.g. hypoxanthine

Definitions

• the present invention provides a novel pyrazolo[3,4-d]pyrimidin-4-one, specifically a derivative of l-(pyridin-4-yl)-pyrazolo[3,4-d]pyrimidin-4-one.
• This compound is a kinase inhibitor that shows unexpected anti-pro liferative activity against cells, including against tumor cells, and anti-tumor activity in xenograft tumor models.
• the compound or a suitable salt or prodrug thereof is useful for the treatment of individuals suffering from a cancer or another proliferative disorder or disease.
• Kinases are important cellular enzymes that perform essential cellular functions such as regulating cell division and proliferation, and that appear to play a decisive role in many disease states such as in disease states that are characterized by uncontrolled proliferation and differentiation of cells. These disease states encompass a variety of cell types and maladies such as cancer, atherosclerosis, restenosis and other proliferative disorders.
• kinase catalytic subunit
• cyclin regulatory subunit
• CDKs cyclin-dependent kinases
• Each transition of the cell cycle is regulated by a particular cyclin-dependent kinase complex: Gl/S by CDK2/CycE, CDK4/CycD and CDK6/CycD; S/G2 by CDK2/CycA and CDKl/CycA; G2/M by CDKl/CycB (for review see Shapiro, J. Clin. Oncol. 24: 17OfF, 2006).
• the coordinated activity of these kinase complexes guides the individual cells through the replication process and ensures the vitality of each subsequent generation (Sherr, Cell 73:1059-1065, 1993; Draetta, Trends Biochem. Sci. 15:378-382, 1990).
• inhibitors include pl6INK4 (an inhibitor of CDK4/CycDl), p2 ICIPl (a general CDK inhibitor), and p27KIPl (a specific CDK2/CycE inhibitor).
• pl6INK4 an inhibitor of CDK4/CycDl
• p2 ICIPl a general CDK inhibitor
• p27KIPl a specific CDK2/CycE inhibitor.
• a crystal structure of p27 bound to CDK2/CycA revealed how these proteins effectively inhibit the kinase activity through multiple interactions with the cyclin-dependent kinase complex (Pavletich, Nature 382:325-331, 1996).
• These protein inhibitors help to regulate the cell cycle through specific interactions with their corresponding cyclin-dependent kinase complexes. Cells deficient in these inhibitors are prone to unregulated growth and tumor formation.
• CDK7 and CDK9 are known to phosphorylate the C-terminal domain of RNA Polymerase II and thereby drive the expression of anti-apoptotic proteins, D-type cyclins, and pro-angiogenesis factors (like hypoxia-induced VEGF). Therefore, also these so-called regulatory CDKs are attractive targets for therapeutic intervention (see Shapiro, J. Clin. Oncol. 24:1770ff, 2006).
• CDK inhibitors Due to the clear connection between pharmacological CDK inhibition and cell cycle regulation, the potential use of CDK inhibitors in oncology was identified early on, and many small-molecule inhibitors belonging to different structural classes were identified (e.g., staurosporins, flavones (e.g., flavopiridol (see Shapiro, J. Clin. Oncol. 24:170ff, 2006)), purines (e.g., purvalanol; roscovitine (see Bach et al, J. Biol. Chem.
• CDK2 activity should result in arrest of cells in Gl, however, it has been shown genetically in colon cancer cells that this block could be by-passed by CDK4 activity (Tetsu & McCormick, Cancer CeIO: 233-45, 2003).
• Inhibition of CDKl/CycB should block exit from mitosis through inhibiting phosphorylation of components of the anaphase-promoting complex (Golan et al., J Biol Chem. 277: 15552-7, 2002; Listovsky et al., Exp Cell Res.
• CDK inhibitory activities e.g., as determined in biochemical kinase inhibition assays in vitro
• ADMET profile including solubility and permeability
• cell biology studies including cellular inhibition of disease-related cell lines
• pharmacokinetic studies including bioavailability
• pharmacodynamic studies including, most importantly, activity in disease-related animal models
• PCT publication WO 00/021926 broadly discloses a class of pyrazolo[3,4- d]pyrimidin-4-ones, including generic structures representing certain 1 -phenyl- and 1- pyridyl-pyrazolo[3,4-d]pyrimidin-4-ones.
• 1-pyridyl-substituted compounds are disclosed or their synthesis described, and no data are presented to indicate whether such compounds would exhibit inhibitory properties in, for example, CDK enzymatic assays.
• PCT publication WO 03/033499 specifically discloses certain l-phenyl-3-isopropyl- 6-arylmethyl-pyrazolo[3,4-d]pyrimidin-4-ones that are shown to inhibit cyclin-dependent kinases and to have certain activity in tumor models (see also WO 2004/092139; WO
• low molecular weight kinase inhibitor compounds with balanced CDK inhibitory activities in biochemical kinase inhibition assays in vitro against CDKl, CDK2, CDK4, CDK6, CDK7 and CDK9, that show potent activity in a xenograft tumor model, and that are useful for treating cancer.
• Such compounds may additionally be useful for treating a wide variety of diseases, including cancer, tumors and other proliferative disorders or diseases including restenosis, angiogenesis, diabetic retinopathy, psoriasis, surgical adhesions, macular degeneration, and atherosclerosis.
• compositions, pharmaceuticals and/or medicaments with kinase inhibitor activity or anti-pro liferative activity against cells such as tumor cells.
• Such compositions, pharmaceuticals and/or medicaments may possess not only such activities, but may also exert tolerable, acceptable or diminished side effects in comparison to other kinase inhibitors or anti-proliferative agents.
• the spectrum of tumor types or other diseases responsive to treatment with such compositions, pharmaceuticals and/or medicaments may be broad.
• compositions, pharmaceuticals and/or medicaments may be suitable for use in the treatment of the mentioned indications as single agent, and/or in combination therapy, be it in connection with other therapeutic agents, with radiation, with operative/surgical procedures, thermal therapy or any other treatment known in the mentioned indications.
• a novel pyrazolo[3,4-d]pyrimidin-4-one specifically a derivative of l-(pyridin-4-yl)-pyrazolo[3,4-d]pyrimidin-4-one having potent and balanced CDK inhibitory activities, particularly against CDKl, CDK2, CDK4, CDK6, CDK7 and CDK9, anti- proliferative activity against tumor cells, good pharmacokinetic and pharmacodynamic properties, including high solubility, permeability and oral bioavailability, and that exhibits surprisingly potent anti-proliferative activity in xenograft tumor models in nude mice, including in models using A2780 tumor cells.
• This compound shows promise as an effective therapeutic for diseases associated with CDK de-regulation or cellular proliferation, such as cancer, or other proliferative disorders or diseases, based on testing against established cancer cell lines and in vivo xenograft cancer models.
• the compound of the present invention is suitable for the treatment of individuals suffering from one or more forms of cancer or for the treatment of individuals suffering from one or more of a variety of other proliferative disorders and diseases.
• the various aspects of the invention relate to the subject compound l-(3,5- dichloropyridin-4-yl)-6-(3-hydroxy-4-pyrrolidinomethyl-phenyl)methyl-3-isopropyl- pyrazolo[3,4-d]pyrimidin-4-one, having the structure represented by formula (I) presented below, or the structure of any tautomeric form thereof.
• the subject compound may optionally be formed or used as a salt form, including a pharmaceutically acceptable salt.
• the subject compound is useful as a CDK inhibitor, showing antiproliferative activity against cells, including against tumor cells, and anti-tumor activity in xenograft tumor models, and/or is useful for the treatment of individuals suffering from cancer or another proliferative disorder or disease.
• the invention relates to pharmaceutical compositions, including a pharmaceutically acceptable diluent, excipient or carrier and an amount, such as a therapeutically effective amount, of such subject compound, e.g., which ameliorates the effects of cancer, or other proliferative disorders or diseases.
• Another aspect of the invention relates to a packaged pharmaceutical product, including a pharmaceutical composition including the subject compound such as described above, and instructions which indicate that said pharmaceutical composition may be used for administration to a patient suffering from cancer, or another proliferative disorder or disease.
• the invention in another aspect, relates to methods that involve administering to or contacting an individual, a cell, a tissue, an organ or an organism with an amount of, including a therapeutically effective amount, of the subject compound or of a pharmaceutical composition disclosed herein.
• methods include, but are not limited to: killing, or inhibiting the proliferation or growth of, a cell (including of a tumor cell); treatment of an individual suffering from a disorder associated with the activity of one or more cyclin dependent kinases disclosed herein; treatment of an individual suffering from cancer; or the treatment of an individual suffering from another proliferative disorder or disease.
• the invention relates to uses of the subject compound for the preparation of a medicament for the treatment of an individual suffering from cancer, or another proliferative disorder or disease.
• Another aspect of the invention relates to a method of synthesizing l-(3,5- dichloropyridin-4-yl)-6-(3-hydroxy-4-pyrrolidinomethyl-phenyl)methyl-3-isopropyl- pyrazo Io [3 ,4-d]pyrimidin-4-one.
• the present invention provides a subject compound that is (i) the compound of formula (I),
• the invention further provides, any salt form of the subject compound, including any pharmaceutically acceptable salt, or any solvate of the subject compound, including any hydrate.
• the invention further provides prodrug forms of the subject compound.
• Figure 1 Effects of twice-daily oral administration of Compound I or Compound P (and the controls Vehicle and Cytoxan) on: (a) A2780 ovarian xenograft tumor growth; and (b) mice body weight. Mean values for each group of mice is plotted at each time-point.
• Figure 2 Fitted values following linear-regression on log-linear plotted data for various groups of mice inoculated with A2780 cells and administered Compound P, Compound I, Vehicle or Cytoxan.
• Figure 3 Effects of daily oral administration of Compound I or Compound P (and the controls Vehicle and R547) on: (a) A2780 ovarian xenograft tumor growth; and (b) mice body weight. Mean values for each group of mice is plotted at each time-point.
• Figure 4 Effects of daily oral administration of Compound I or Compound P (and the controls Vehicle and R547) on: (a) H460 lung xenograft tumor growth; and (b) mice body weight. Mean values for each group of mice is plotted at each time-point.
• Figure 5 Effects of daily oral administration of Compound I or Compound P (and the controls Vehicle and R547) on: (a) HCTl 16 colon xenograft tumor growth; and (b) mice body weight. Mean values for each group of mice is plotted at each time-point.
• Figure 6 Inhibition of CDK9-dependent RNAPII phosphorylation in HCTl 16 cells by compound I. Also shown are total RNAPII and tubulin protein as controls. Cells and lysates were processed as described in Example D.
• tautomer is art recognised, and describes an isomer of a given compound that results from the formal migration of a hydrogen atom or proton, accompanied by a switch of a single bond and adjacent double bond.
• tautomer includes any possible tautomeric form of the compound of the present invention of formula (I). It is further understood that different tautomeric forms of a compound may coexist in solution or in the solid state, e.g. in an equilibrium state, and that one tautomeric form may converted into a different tautomeric form over time.
• a reference to the compound of formula (I) is to be understood to refer to (i) the compound having the structure represented by formula
• isotopes refers to one of a set of compounds having identical molecular formulas but differing in the nature or sequence of bonding of their atoms or in the arrangement of their atoms in space.
• the present invention is intended to include all isotopes of atoms occurring on the present compound.
• Isotopes are atoms having the same atomic number but different mass numbers.
• isotopes of hydrogen include 1 H, 2 H (deuterium) and 3 H (tritium).
• isotopes of carbon include 12 C and 14 C.
• active ingredient refers to an agent that is included in a pharmaceutical composition, and that is an active agent per se, or that results in the formation of an active agent such as, by way of non-limiting examples, an active ingredient being a prodrug, or an active ingredient that leads to the formation of a metabolite that is the active agent.
• active agent refers to an agent that is responsible for, or is essentially contributing to, the desired activity, such as a therapeutic activity, of a method, such as a therapeutic method, using such agent, e.g., treatment of an individual upon administration of a pharmaceutical composition comprising an active ingredient.
• An active agent may exert its activity either alone or in combination with one or more additional active agents.
• the active agent may or may not be known, e.g., it may not be known whether an active ingredient acts directly or indirectly, such as an action mediated via a metabolite of the active ingredient. Without intending to be bound by a particular theory, it is believed that the compound of the present invention is an active agent as such.
• agent refers to any substance that exerts, or is supposed to exert, a certain activity in the setting described in the context where the term “agent” is used, either directly or indirectly.
• metabolic refers to any substance produced by metabolism or by a metabolic process. Metabolism, as used herein, refers to the various physical/chemical/biochemical/pharmacological reactions (each being a "metabolic process") involved in the transformation of a molecule or chemical compound occurring in the cell, tissue, system, or individual, including a human, that is contacted with such molecule or chemical compound.
• IC 50 refers to concentrations at which a measurable activity, phenotype or response, for example growth or proliferation of cells such as tumor cells, is inhibited by 50%. IC50 values can be estimated from an appropriate dose-response curve, for example by eye or by using appropriate curve fitting or statistical software. More accurately, IC50 values may be determined using non-linear regression analysis.
• an "individual” means a multi- cellular organism, for example an animal such as a mammal, including a primate. In addition to primates, such as humans, a variety of other mammals can be comprised within the term individual.
• mammals including, but not limited to, cows, sheep, goats, horses, dogs, cats, guinea pigs, rabbits, rats, mice or other bovine, ovine, equine, canine, feline, or rodent species can be used.
• a proliferative disorder or a “proliferative disease” includes a disorder or disease that is characterized by proliferation of cells in an individual beyond or above normal levels, that is, above that level of proliferation of cells seen in normal, non- diseased cells of the same type.
• a proliferative disorder or disease may be caused or affected by changes in cellular growth, differentiation, or proliferation processes, including aberrantly regulated cellular growth, proliferation, differentiation, or migration of cells.
• Proliferation disorders or diseases include tumorigenic disorders or diseases. Examples of cellular growth or proliferation disorders include, but are not limited to tumors, cancer, autoimmune diseases, viral diseases, fungal diseases, neurodegenerative disorders and cardiovascular diseases.
• Cells included in, comprising or derived from a tumor or a cancer will generally be understood to be proliferating cells, typically hyper-proliferating cells, and in other circumstances, a tumor cell may be dysplastic, or may have proliferated.
• cellular growth, differentiation or proliferation processes are processes by which cells increase in number, size or content, by which a cell develops a specialized set of characteristics which differ from that of other cells, or by which a cell moves closer to or further from a particular location or stimulus, including amino acid transport and degradation and other metabolic processes of a cell.
• a "tumorigenic disorder or disease” includes a disorder or disease characterized by aberrantly regulated cellular growth, proliferation, differentiation, adhesion, or migration, which may result in the production of or tendency to produce tumors.
• a “tumor” includes a benign or malignant mass of tissue, e.g. as formed by abnormal proliferation of cells.
• anti-cancer agent refers to an active agent having the ability to kill cancer cells in vivo or in vitro, to induce apoptosis in cancer cells, to inhibit
• antiproliferative agent refers to an active agent having the ability to inhibit proliferation of growing cells in vivo or in vitro, or to reduce the rate of cell division in proliferating or hyper-proliferating cells.
• agents are known to be anti-cancer agents and/or anti-proliferative agents, and such known agents include, but are not limited to, altretamine, busulfan, chlorambucil, cyclophosphamide, ifosfamide, mechlorethamine, melphalan, thiotepa, cladribine, fluorouracil, floxuridine, gemcitabine, thioguanine, pentostatin, methotrexate, 6-mercaptopurine, cytarabine, carmustine, lomustine, streptozotocin, carboplatin, cisplatin, oxaliplatin, picop latin, LA- 12, iproplatin, tetraplatin, lobaplatin, JM216, JM335, satraplatin, fludarabine, aminoglutethimide, flutamide, goserelin, leuprolide, megestrol acetate, cyproterone acetate,
• non-small molecule therapeutics such as antibodies, e.g., 1D09C3 and other anti- HLA-DR antibodies as described in WO 01/87337 and WO 01/97338, Rituxan as described in US patents 5,736,137, 5,776,456, 5,843,437, 4D5, Mab225, C225, Daclizumab (Zenapax), Antegren, CDP 870, CMB-401, MDX-33, MDX-220, MDX-477, CEA-CIDE, AHM, Vitaxin, 3622W94, Therex, 5Gl.1, IDEC-131, HU-901, Mylotarg, Zamyl (SMART M195), MDX-210, Humicade, LymphoCIDE, ABX-EGF, 17- IA, Trastuzumab (Herceptin ® , rhuMAb), Epratuzumab, Cetuximab (Erbitux ® ), Per
• phrases "pharmaceutically acceptable” is art recognized, and is understood to refer to a property of a compound, material, composition, dosage form, etc. which property renders such compound, material, composition, dosage form, etc., suitable, within the scope of sound medical judgment, for administration to, or use in the pharmaceutical treatment of, an individual by providing a medically acceptable benefit/risk ratio. Such sound medical judgment should exclude, for example, that by bringing such compound, material, composition, dosage form, etc. in contact with the tissues of human beings or animals there is excessive toxicity, irritation, allergic response, or other problem or complication not commensurate with the benefits provided. Thus, “pharmaceutically acceptable” is used to refer to compounds, materials, compositions, dosage forms, etc.
• salt is art recognized, and is understood to refer to those derivatives of a parent compound wherein such parent compound is modified by making acid or base salts thereof, and where such salt is pharmaceutically acceptable.
• a pharmaceutically acceptable salt will usually maintain, or even improve on, the balance between the desired biological activity of an active ingredient and its undesired or toxicological effects.
• Acid salts include salts from the reaction of mineral or organic acids with basic residues in the parent compound, such as amines.
• Non-limiting examples of suitable acid salts include those derived from inorganic acids, such as, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, sulfamic acid, phosphoric acid, nitric acid, bicarbonic acid, carbonic acid; and acid salts formed with organic acids, such as, for example, formic acid, acetic acid, propionic acid, oxalic acid, gly colic acid, stearic acid, lactic acid, tartaric acid, succinic acid, malic acid, maleic acid, hydroxymaleic acid, malonic acid, ascorbic acid, citric acid, glutamic acid, benzoic acid, tannic acid, palmoic acid, alginic acid, phenylacetic acid, polyglutamic acid, salicylic acid, sulfanilic acid, 2-acetoxybenzoic acid, fumaric acid, toluenesulfonic,acid, methanesulfonic acid, ethane disulf
• the salt form of the subject compound is a hydrochloride.
• the salt form of the subject compound is a maleate, including the particular embodiment of such salt comprising the subject compound and maleic acid in a 1 :1 ratio.
• Base salts include salts from the reaction of alkali metal or alkaline earth metal bases with acidic residues on the parent compound, such as carboxylic acids, phenolic residues, and the like.
• Suitable alkali metal or alkaline earth metal bases include lithium, potassium and sodium hydroxide, or calcium and magnesium hydroxide.
• Other suitable bases include those derived from metals such as zinc, bismuth, barium, or aluminum, and further include ammonia and organic amines, such as N,N-dibenzylethylene-diamine, D-glucosamine, or ethylenediamine.
• suitable salts include those derived from a combination of acids and bases, such as, for example, a zinc tannate salt.
• salts can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemical methods. Generally, such salts can be prepared by reacting the free acid or base forms of the compounds with a stoichiometric amount or a slight excess of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, EtOAc, ethanol, isopropanol, or acetonitrile are preferred. Lists of suitable salts are found in Remington's Pharmaceutical Sciences, 18th ed., Mack Publishing Company, Easton, PA, 1990, p. 1445, the disclosure of which is hereby incorporated by reference.
• Non-pharmaceutically acceptable acids and bases that find use, for example, in the synthesis and/or purification of a compound of interest are also contemplated herein.
• all “salts" of the compound of formula (I) are also encompassed within the scope of the instant invention.
• prodrug refers to an active ingredient that is converted into an active agent in one or more steps upon administration of a pharmaceutical composition including the prodrug.
• prodrugs are derivatives of per se drugs that after administration to an individual, or to cells obtained from an individual, undergo conversion or metabolism to the physiologically active species, i.e. the active agent.
• the conversion may be spontaneous, such as hydrolysis in the physiological environment, or may be enzyme-catalyzed.
• Prodrugs include compounds that can be oxidized, reduced, aminated, deaminated, hydroxylated, dehydroxylated, hydrolyzed, esterif ⁇ ed, alkylated, dealkylated, acylated, deacylated, phosphorylated, and/or dephosphorylated to finally result in the active agent.
• a prodrug may also be any covalently bonded carrier that releases an active agent in vivo when such carrier is administered as an active ingredient to an individual.
• Prodrugs can enhance numerous desirable qualities of pharmaceuticals (e.g., solubility, bioavailability, manufacturing, transport, pharmacodynamics, etc.).
• Prodrugs may be bioavailable by oral administration even when the parent drug is not.
• Prodrugs may be prepared by modifying a functional group present in the parent compound in such a way that the modifications are reversible, for example in the course of administering the prodrug or in vivo, to result in the parent compound.
• administered means providing a compound to an individual, including a human, by bringing such individual in contact with, or otherwise exposing such individual to, such compound in such a manner that such compound has the opportunity to exert its activity, such as a therapeutic activity for the benefit of an individual suffering from a disease or disorder, or in need of treatment.
• m vitro refers to a biological entity, a biological process, or a biological reaction outside the body in artificial conditions.
• a cell grown in vitro is to be understood as a cell grown in an environment outside the body, e.g., in a test tube, a culture tray, or a microtiter plate.
• therapeutically effective amount is art recognized, and is understood to mean the amount of the subject compound that will elicit the biological, physiological, pharmacological, therapeutic or medical response of a cell, tissue, system, or individual, including a human, that is being sought by the practitioner administering said amount.
• such desired results may include, e.g., lessening of the effects/symptoms of a disorder or disease, such as a proliferative disorder or disease, for example, a cancer or tumor, or killing or inhibiting growth of a proliferating cell, such as a tumor cell.
• a disorder or disease such as a proliferative disorder or disease, for example, a cancer or tumor
• killing or inhibiting growth of a proliferating cell such as a tumor cell.
• the therapeutically effective amount can be determined by standard procedures, including those described below in the section "Dosages" herein.
• treatment is art recognized, and shall include any one or more form of therapeutic intervention of a condition, such as any disorder or disease, of cells or of an individual, including therapeutic intervention at a symptom or associated-disorder of such condition, and further includes adjuvant therapy that is given after a substantial portion of the detectable or accessible manifestation of the condition, disorder or disease has undergone prior therapeutic intervention, e.g., by surgery.
• a beneficial treatment may prevent onset of a disease or a symptom of it, may alleviate a disease or one or more symptoms or manifestations of it, may delay or inhibit the development or spread of a disease or one or more symptoms or manifestations of it, may cause regression of a disease or one or more symptoms or manifestations of it, or may cure a disease or eliminate one or more symptoms or manifestations of a disease.
• Treatment is not synonymous with "cure”.
• further treated means that different active ingredients, therapeutic agents or compounds may be administered together, sequentially, alternatively, or intermittently. Such further administration may be temporally or spatially separated, for example, at different times, on different days, or via different modes or routes of administration.
• the compound of the invention is isolated.
• the compound of the invention, or a pharmaceutically acceptable salt thereof is purified, e.g., to have a purity selected from: at least 80%, at least 90%, at least 95%, at least 97%, at least 98% and in certain embodiments at least 99%.
• Purity can refer to either absolute or relative purity. Absolute purity refers to the amount of compound of interest in relation to the total amount of a composition including such compound.
• Relative purity refers to the amount of a compound of interest in a composition relative to the amount of one or more other substances included in such composition, e.g. one or more impurities such as by-products, degradation products (e.g., metabolites, products of oxidation or hydrolysis, etc.) and/or compounds that degrade to form the compound of the invention (e.g., precursors or prodrugs).
• impurities such as by-products, degradation products (e.g., metabolites, products of oxidation or hydrolysis, etc.) and/or compounds that degrade to form the compound of the invention (e.g., precursors or prodrugs).
• Such other substance(s) may, for example, be present in the product of a synthetic chemistry scheme for such compound of interest.
• absolute purity refers to the amount of the compound of interest relative to all others components of a composition including such compound, while relative purity is mainly used to describe purity with regard to closely related substances, and thus is unaffected by the addition of unrelated compounds, such as excipients, stabilizers, or other medicaments for conjoint administration.
• Purity can be assessed based upon weight, volume or molar ratios of one compound relative to others. Purity can be measured by a variety of analytical techniques, including elemental abundance, UV-visible spectrometry, HPLC, GC- MS, NMR, mass spectrometry, and thin layer chromatography. Preferred methods for determining the purity of compounds according to the invention are by HPLC, GC-MS, or NMR.
• the compound of the invention is synthetically produced.
• synthetically produced refers to the generation of a compound using synthesis techniques well known to the skilled artisan with the aim of obtaining such compound.
• the compound of the invention, a salt form thereof, including a pharmaceutically acceptable salt, or a solvate form thereof, including a hydrate is in amorphous form. In certain embodiments, the compound of the invention, a salt form thereof, including a pharmaceutically acceptable salt, or a solvate form thereof, including a hydrate, is in crystalline form.
• prodrugs of the subject compound or a salt form thereof include compounds, wherein the hydroxyl group at the phenyl substituents or any nitrogen atom in the pyrrolidino group, the pyridine substituent or the pyrazolopyrimidone ring system of the compound of the present invention is bonded to any group that, when such prodrug is administered to cells, tissue, or an individual, including a human, is cleaved to form the free hydroxyl group or nitrogen atom, respectively.
• prodrugs include, but are not limited to, acetate, formate, and benzoate derivatives of the phenolic hydroxy group in the compound of the present invention, or an N- acyloxyalkyl pyridinium derivative of the compound of the present invention (Davidsen et al, J Med Chem. 1994; 37:4423-9).
• the present invention further provides a pharmaceutical composition including an active ingredient selected from: the subject compound, a salt form, such as a pharmaceutically acceptable salt, of the subject compound and a prodrug thereof; together with a pharmaceutically acceptable diluent, excipient or carrier, including pharmaceutical compositions including a therapeutically effective amount of such active ingredient.
• a pharmaceutical composition including an active ingredient selected from: the subject compound, a salt form, such as a pharmaceutically acceptable salt, of the subject compound and a prodrug thereof; together with a pharmaceutically acceptable diluent, excipient or carrier, including pharmaceutical compositions including a therapeutically effective amount of such active ingredient.
• compositions of this invention can be formulated and administered to treat cells, or an individual, such as a human, including an individual in need of treatment, such as an individual suffering from a disorder like a cancer.
• Such formulation and administration can be by any appropriate means that produces contact of the active agent with the active agent's site of action, such as a cell in the body of such individual, e.g., a cell included in a tumor or cancer present within such individual.
• They can be administered by any conventional means available for use in conjunction with pharmaceuticals, either as individual therapeutically active ingredients or in a combination of therapeutically active ingredients. They can be administered alone, but are generally administered with a pharmaceutically acceptable diluent, excipient or carrier selected on the basis of the chosen route of administration and standard pharmaceutical practice.
• compositions for use in accordance with the present invention may be formulated in conventional manner using one or more pharmaceutically acceptable diluents, excipients or carriers.
• the pharmaceutical compositions of the invention can be formulated for a variety of routes of administration, including systemic and topical or localized administration. Techniques and formulations generally may be found in Remington's Pharmaceutical Sciences, Meade Publishing Co., Easton, PA.
• the pharmaceutical compositions of the present invention may be specially formulated for administration in solid or liquid form, including those adapted for the following: (1) oral administration, for example, drenches (aqueous or non-aqueous solutions or suspensions), tablets, capsules, boluses, powders, granules, pastes for application to the tongue; (2) parenteral administration, for example, by subcutaneous, intramuscular or intravenous injection as, for example, a sterile solution or suspension; (3) topical application, for example, as a cream, ointment or spray applied to the skin; or (4) intravaginally or intrarectally, for example, as a pessary, cream or foam.
• the pharmaceutical preparations may be non-pyrogenic, i.e., do not substantially elevate the body temperature of a patient.
• compositions of the present invention are formulated for oral administration.
• compositions of the present invention are formulated for intravenous administration.
• wetting agents such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, release agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the compositions.
• antioxidants examples include: (1) water soluble antioxidants, such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulf ⁇ te, sodium sulfite and the like; (2) oil-soluble antioxidants, such as ascorbyl palmitate, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), lecithin, propyl gallate, alpha-tocopherol, and the like; and (3) metal chelating agents, such as citric acid, ethylenediamine tetraacetic acid (EDTA), sorbitol, tartaric acid, phosphoric acid, and the like.
• water soluble antioxidants such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulf ⁇ te, sodium sulfite and the like
• oil-soluble antioxidants such as ascorbyl palmitate, butylated hydroxyanisole (BHA), butylated hydroxytoluene (
• Formulations of the present invention include those suitable for oral, nasal, topical (including buccal and sublingual), rectal, vaginal and/or parenteral administration.
• the formulations may conveniently be presented in unit dosage form and may be prepared by any methods well known in the art of pharmaceutics.
• the amount of active ingredient which can be combined with a carrier material to produce a single dosage form will vary depending upon the individual being treated, as well as the particular mode of administration.
• the amount of active ingredient which can be combined with a carrier material to produce a single dosage form will generally be that amount of active ingredient which produces a therapeutic effect.
• this amount will range from about 1 percent to about ninety-nine percent by weight of active ingredient, in certain embodiments from about 5 percent to about 70 percent by weight, and in particular such embodiments from about 10 percent to about 30 percent by weight.
• Methods of preparing these formulations or compositions include the step of bringing into association the compound of the present invention, or a prodrug of such compound, with the carrier and, optionally, one or more accessory ingredients.
• the formulations are prepared by uniformly and intimately bringing into association the compound of the present invention, or a prodrug of such compound, with liquid carriers, or finely divided solid carriers, or both, and then, if necessary, shaping the product.
• injection is preferred, including intramuscular, intravenous, intraperitoneal, and subcutaneous (i.m., i.v., i.p., and s.c, respectively).
• systemic administration means the administration of a compound, drug or other material other than directly into the central nervous system, such that it enters the patient's circulatory system and, thus, is subject to metabolism and other like processes.
• compositions of the invention can be formulated in liquid solutions, preferably in physiologically compatible buffers such as Hank's solution or
• compositions may be formulated in solid form and redissolved or suspended immediately prior to use. Lyophilized forms are also included.
• compositions of the invention may be formulated to be suitable for oral administration and may take the form of capsules, cachets, sachets, pills, tablets, lozenges (using a flavored base, such as sucrose and acacia or tragacanth), powders, granules, or as a solution or a suspension in an aqueous or non-aqueous liquid, or as an oil-in-water or water-in-oil liquid emulsion, or as an elixir or syrup, or as pastilles (using an inert base, such as gelatin and glycerin, or sucrose and acacia) and/or as mouth washes and the like, each containing a predetermined amount of a compound of the present invention, or a prodrug of such compound, as an active ingredient.
• a flavored base such as sucrose and acacia or tragacanth
• the compound of the present invention, or a prodrug of such compound may also be administered as a bolus, electuary or paste.
• the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug of such compound, as active ingredient is mixed with one or more pharmaceutically acceptable carriers, such as sodium citrate or dicalcium phosphate, and/or any of the following, or combinations thereof: (1) fillers or extenders, such as starches, lactose, sucrose, glucose, mannitol, and/or silicic acid; (2) binders, such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinyl pyrrolidone, sucrose and/or acacia; (3) humectants, such as glycerol; (4) disintegrating agents, such as agar-a
• compositions may also comprise buffering agents.
• Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugars, high molecular weight polyethylene glycols, and the like.
• Gelatin capsules can contain the compound of the present invention, or a prodrug of such compound, as active ingredient and powdered carriers, such as lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like. Similar carriers can be used to make compressed tablets. Both tablets and capsules can be manufactured as sustained- release products to provide for continuous release of medication over a period of hours. Compressed tablets can be sugar-coated or film-coated to mask any unpleasant taste and protect the tablet from the atmosphere, or enteric coated for selective disintegration in the gastrointestinal tract.
• powdered carriers such as lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like. Similar carriers can be used to make compressed tablets. Both tablets and capsules can be manufactured as sustained- release products to provide for continuous release of medication over a period of hours. Compressed tablets can be sugar-coated or film-coated to mask any unpleasant taste and protect the tablet from the atmosphere, or enteric coated for selective disintegration in the
• Solid compositions of a similar type are also employed as fillers in soft and hard-filled gelatin capsules; preferred materials in this connection also include lactose or milk sugar as well as high molecular weight polyethylene glycols.
• a particular example of such formulation is a solution or suspension in an oil, for example olive oil, Miglyol, or Capmul, contained within a soft gelatin capsule.
• Antioxidants may be added to prevent long- term degradation as appropriate.
• a tablet may be made by compression or molding, optionally with one or more ancillary ingredients.
• Compressed tablets may be prepared using a binder (for example, gelatin or hydroxypropylmethyl cellulose), lubricant, inert diluent, preservative, disintegrant (for example, sodium starch glycolate or cross-linked sodium carboxymethyl cellulose), surface-active or dispersing agent.
• a binder for example, gelatin or hydroxypropylmethyl cellulose
• lubricant for example, gelatin or hydroxypropylmethyl cellulose
• inert diluent preservative
• disintegrant for example, sodium starch glycolate or cross-linked sodium carboxymethyl cellulose
• surface-active or dispersing agent for example, hydroxypropylmethyl cellulose
• Molded tablets may be made by molding in a suitable machine a mixture of the powdered composition moistened with an inert liquid diluent.
• the tablets and other solid dosage forms of the pharmaceutical compositions of the present invention may optionally be scored or prepared with coatings and shells, such as enteric coatings and other coatings well known in the pharmaceutical-formulating art. They may also be formulations so as to provide slow or controlled release of the active ingredient therein using, for example, hydroxypropylmethyl cellulose in varying proportions to provide the desired release profile, other polymer matrices, liposomes and/or microspheres.
• compositions may be sterilized by, for example, filtration through a bacteria-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved in sterile water, or some other sterile injectable medium immediately before use.
• These compositions may also optionally contain opacifying agents and may be of a composition that they release the active ingredient(s) only, or in certain embodiments, in a certain portion of the gastrointestinal tract, optionally in a delayed manner.
• embedding compositions which can be used include polymeric substances and waxes.
• the active ingredient can also be in micro-encapsulated form, if appropriate, with one or more of the above-described excipients.
• Liquid dosage forms for oral administration of the pharmaceutical compositions of the invention include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups, and elixirs.
• the liquid dosage forms may contain inert diluents commonly used in the art, such as, for example, water or other solvents, solubilizing agents and emulsif ⁇ ers, such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor and sesame oils), glycerol, tetrahydrofuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
• inert diluents commonly used in the art, such as, for example, water or other solvents, solub
• the pharmaceutical compositions for oral administration can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, coloring, perfuming, and preservative agents.
• Suspensions in addition to the pharmaceutical composition of the present invention, may contain suspending agents as, for example, ethoxylated isostearyl alcohols, polyoxy ethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, and tragacanth, and mixtures thereof.
• the pharmaceutical compositions may take the form of tablets or lozenges formulated in a conventional manner.
• the pharmaceutical compositions of the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebuliser, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas.
• a suitable propellant e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas.
• a suitable propellant e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas.
• a suitable propellant e.g., dichlorodifluoromethane, trichlor
• the pharmaceutical compositions may be formulated for parenteral administration by injection, e.g., by bolus injection or continuous infusion.
• Formulations for injection may be presented in unit dosage form, e.g., in ampoules or in multi-dose containers, with an added preservative.
• the pharmaceutical compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents.
• the active ingredient may be in powder form for constitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use.
• parenteral administration and “administered parenterally” as used herein means modes of administration other than enteral and topical administration, usually by injection, and includes, without limitation, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal and intrasternal injection and infusion.
• compositions of this invention suitable for parenteral administration comprise the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug of such compound, in combination with one or more pharmaceutically acceptable sterile isotonic aqueous or nonaqueous solutions, dispersions, suspensions or emulsions, or sterile powders which may be reconstituted into sterile injectable solutions or dispersions just prior to use, which may contain antioxidants, buffers, bacteriostats, solutes which render the formulation isotonic with the blood of the intended recipient or suspending or thickening agents.
• aqueous and nonaqueous carriers examples include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, and the like), and suitable mixtures thereof, vegetable oils, such as olive oil, and injectable organic esters, such as ethyl oleate.
• polyols such as glycerol, propylene glycol, polyethylene glycol, and the like
• vegetable oils such as olive oil
• injectable organic esters such as ethyl oleate.
• Proper fluidity can be maintained, for example, by the use of coating materials, such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants.
• These pharmaceutical compositions may also contain adjuvants such as preservatives, wetting agents, emulsifying agents and dispersing agents.
• microorganisms Prevention of the action of microorganisms may be ensured by the inclusion of various antibacterial and antifungal agents, for example, paraben, chlorobutanol, phenol sorbic acid, and the like. It may also be desirable to include isotonic agents, such as sugars, sodium chloride, and the like into the pharmaceutical compositions. In addition, prolonged absorption of the injectable pharmaceutical form may be brought about by the inclusion of agents that delay absorption such as aluminum monostearate and/or gelatin.
• antibacterial and antifungal agents for example, paraben, chlorobutanol, phenol sorbic acid, and the like.
• isotonic agents such as sugars, sodium chloride, and the like
• prolonged absorption of the injectable pharmaceutical form may be brought about by the inclusion of agents that delay absorption such as aluminum monostearate and/or gelatin.
• the pharmaceutical compositions may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection.
• the pharmaceutical compositions may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.
• Systemic administration can also be by transmucosal or transdermal means. For transmucosal or transdermal administration, penetrants appropriate to the barrier to be permeated are used in the formulation.
• Such penetrants are generally known in the art, and include, for example, for transmucosal administration bile salts and fusidic acid derivatives.
• detergents may be used to facilitate permeation.
• Transmucosal administration may be through nasal sprays or using suppositories.
• the pharmaceutical compositions of the invention are formulated into ointments, salves, gels, or creams as generally known in the art.
• the rate of absorption of the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug of such compound then depends upon its rate of dissolution which, in turn, may depend upon crystal size and crystalline form.
• delayed absorption of a parenterally administered inhibitor form is accomplished by dissolving or suspending the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug of such compound, in an oil vehicle.
• compositions of the invention may be formulated for rectal or vaginal administration as a suppository, which may be prepared by mixing the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug of such compound, with one or more suitable nonirritating excipients or carriers comprising, for example, cocoa butter, polyethylene glycol, a suppository wax or a salicylate, and which is solid at room temperature, but liquid at body temperature and, therefore, will melt in the rectum or vaginal cavity and release the active ingredient.
• suitable nonirritating excipients or carriers comprising, for example, cocoa butter, polyethylene glycol, a suppository wax or a salicylate, and which is solid at room temperature, but liquid at body temperature and, therefore, will melt in the rectum or vaginal cavity and release the active ingredient.
• Formulations of the pharmaceutical compositions of the present invention which are suitable for vaginal administration, also include pessaries, tampons, creams, gels, pastes, foams or spray formulations containing such carriers as are known in the art to be appropriate.
• Dosage forms for the topical or transdermal administration of the compound of this invention, or a prodrug of such compound include powders, sprays, ointments, pastes, creams, lotions, gels, solutions, patches and inhalants.
• Such compound may be mixed under sterile conditions with a pharmaceutically acceptable carrier, and with any preservatives, buffers, or other adjuvant which may be required.
• the ointments, pastes, creams and gels may contain, in addition to the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug of such compound, excipients, such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acid, talc and zinc oxide, or mixtures thereof.
• Powders can contain, in addition to the compound of this invention, or a prodrug of such compound, excipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and polyamide powder, or mixtures of these substances.
• Transdermal patches have the added advantage of providing controlled delivery of a compound of the present invention to the body.
• dosage forms can be made by dissolving or dispersing an inhibitor of the present invention in the proper medium.
• Absorption enhancers can also be used to increase the flux of the drug across the skin. The rate of such flux can be controlled by either providing a rate-controlling membrane or dispersing the compound of the present invention in a polymer matrix or gel.
• Ophthalmic formulations, eye ointments, powders, solutions and the like, are also contemplated for use in this invention.
• compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the active ingredient.
• the pack may for example comprise metal or plastic foil, such as a blister pack.
• the pack or dispenser device may be accompanied by instructions for administration. In other embodiments, the pack or dispenser may be further packaged in an outer carton.
• a pharmaceutical composition of the present invention can also be formulated as a sustained and/or timed release formulation.
• sustained and/or timed release formulations may be made by sustained release means or delivery devices that are well known to those of ordinary skill in the art, such as those described in U.S. Patent Nos.: 3,845,770; 3,916,899; 3,536,809; 3,598,123; 4,008,719; 4,710,384; 5,674,533; 5,059,595; 5,591,767; 5,120,548; 5,073,543; 5,639,476; 5,354,556; and 5,733,566, the disclosures of which are each incorporated herein by reference.
• compositions of the present invention can be used to provide slow or sustained release of one or more of the active ingredients using, for example, hydroxypropylmethyl cellulose, other polymer matrices, gels, permeable membranes, osmotic systems, multilayer coatings, microparticles, liposomes, microspheres, or the like, or a combination thereof to provide the desired release profile in varying proportions.
• Suitable sustained release formulations known to those of ordinary skill in the art, including those described herein, may be readily selected for use with the pharmaceutical compositions of the invention.
• Injectable depot forms can be made by forming microencapsulated matrices of the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug of such compound, in biodegradable polymers such as polylactide-polyglycolide. Depending on the ratio of drug to polymer, and the nature of the particular polymer employed, the rate of drug release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations can also be prepared by entrapping the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug of such compound, in liposomes or microemulsions that are compatible with body tissue. Dosages
• the dosage to be administered that will be a therapeutically effective amount of the compound sufficient, or reasonably expected by a health-care professional such as a physician, veterinarian, pharmacist or nurse, to result in amelioration of symptoms of, for example, the cancer or tumor will, of course, vary depending upon known factors such as the pharmacodynamic characteristics of the particular active ingredient and its mode and route of administration; age, sex, health and weight of the recipient; nature and extent of symptoms; kind of concurrent treatment, frequency of treatment and the effect desired.
• a dosage comprising a lower dose of the inventive compound, or of a salt or prodrug thereof, than would be therapeutically effective if administered alone may also be used, such as when the subject compound is used in combination with another therapeutically active agent, such as an anti-cancer agent, provided that such combination is therapeutically effective.
• Toxicity and therapeutic efficacy of pharmaceutical compositions of the present invention can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD50 (the dose lethal to 50% of the tested population) and the ED50 (the dose therapeutically effective in 50% of the population).
• the dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD50/ED50.
• Pharmaceutical compositions that exhibit favourable therapeutic indices are useful for many circumstances. In certain circumstances, even pharmaceutical compositions that appear to exhibit debilitating or toxic side effects may be used, including circumstances where care is taken to design a delivery system that targets the active agent of such pharmaceutical compositions to the site of affected tissue in order to minimize potential damage to unaffected cells and, thereby, reduce or localize side effects.
• the dosage lies within a range of circulating concentrations that include the ED50 with little or no toxicity.
• the dosage may vary within this range depending upon the dosage form employed and the route of administration utilized.
• the therapeutically effective dose can be estimated initially from cell culture assays.
• a dose may be formulated in animal models to achieve a circulating plasma concentration range that includes the IC50 (i.e., the concentration of the test active ingredient which achieves a half-maximal inhibition of symptoms or inhibition of biochemical activity) as determined in cell culture.
• IC50 i.e., the concentration of the test active ingredient which achieves a half-maximal inhibition of symptoms or inhibition of biochemical activity
• levels in plasma may be measured, for example, by high performance liquid chromatography.
• an active ingredient depends upon a number of factors known to those skilled in the art, e.g., a physician.
• the dose(s) of the active ingredient will vary, for example, depending upon the identity, size, and condition of the individual or sample being treated, further depending upon the route by which the composition is to be administered, if applicable, and the effect which the practitioner desires the therapeutic to have upon the therapeutic target of targets, such as cells, nucleic acid or polypeptides, through which the disease causes, symptoms or effects are mediated.
• Exemplary doses include milligram or microgram amounts of the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug of such compound, per kilogram of subject or sample weight, e.g., 1 microgram per kilogram to 500 milligrams per kilogram, 100 micrograms per kilogram to 50 milligrams per kilogram, or 1 milligram per kilogram to 5 milligrams per kilogram.
• doses can also be calculated on a body surface basis.
• a person of 70 kg has an approximate body surface area of 1.8 square meter, and doses can be expressed as milligram or microgram amounts of the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug of such compound, per body surface area of subject or sample, e.g., 50 microgram per square meter to 15 grams per square meter, 5 milligrams per square meter to 1.5 grams per square meter, or 50 milligram per square meter to 150 milligrams per square meter.
• the present invention further provides the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug of such compound, as for therapy.
• said therapy is the treatment of an individual suffering from cancer, or another proliferative disorder or disease.
• said treatment is the treatment of an individual suffering from a disorder or disease, such as a cancer, associated with the activity of one or more cyclin dependent kinases, such as by inhibition of the activity of CDKl, CDK2, CDK4, CDK7, and/or CDK9.
• the present invention additionally provides a method for treating an individual, such as a mammal, including a human, having or suffering from cancer or another proliferative disorder or disease, comprising administering to said individual an amount, such as a therapeutically effective amount, of the subject compound, a pharmaceutically acceptable salt thereof, or a prodrug thereof, or administering a pharmaceutical composition of the invention as described above.
• said individual is a human.
• said treatment is the treatment of a cancer that can be treated by the inhibition of the activity of one or more cyclin dependent kinases, such as the inhibition of the activity CDKl , CDK2, CDK4, CDK7, and/or CDK9.
• the invention provides methods of treating an individual suffering from a disease, such as a mammal, including a domestic mammal, rodent, and human, comprising the step of exposing or contacting said individual to an amount, including a therapeutically effective amount, of the subject compound, a pharmaceutically acceptable salt thereof, or a prodrug thereof.
• a disease such as a mammal, including a domestic mammal, rodent, and human
• the disease is a cancer, or another proliferative disorder or disease.
• said individual is a human.
• cells associated with said cancer, or said another proliferative disorder or disease including tumor cells included in a tumor or a cancer (e.g.
• tumor cells that are malignant cell in an individual are exposed to the subject compound, a pharmaceutically acceptable salt thereof, or a prodrug thereof.
• said compound, a pharmaceutically acceptable salt thereof, or a prodrug thereof is administered to said individual in which said cells are present.
• said treatment is the treatment of a cancer that can be treated by the inhibition of the activity of one or more cyclin dependent kinases, such as the inhibition of the activity of CDKl, CDK2, CDK4, CDK7, and/or CDK9.
• the invention provides a method of killing or inhibiting proliferation or growth of cells, comprising contacting the cells with the compound of the invention, a pharmaceutically acceptable salt thereof, or a prodrug thereof.
• the cells are contacted with such agent in vitro, while in an alternative embodiment the cells are present in an individual.
• the cells are cancer cells, for example cells from a tumor cell line or cells included in a tumor or cancer, including cancer cells from a tumor that can be treated by the inhibition of the activity of one or more cyclin dependent kinases, such as the inhibition of the activity of CDKl, CDK2, CDK4, CDK7, and/or CDK9.
• Yet another aspect of the invention relates to the use of the compound as described above, a pharmaceutically acceptable salt thereof, or a prodrug thereof, for the preparation of a medicament for the treatment or prevention of a cancer, or of another proliferative disorder or disease including a cancer that can be treated by the inhibition of the activity of one or more cyclin dependent kinases, such as the inhibition of the activity of CDKl, CDK2, CDK4,
• the invention relates to a pharmaceutical composition
• a pharmaceutical composition comprising an active ingredient selected from: the compound as described above, a pharmaceutically acceptable salt thereof, and a prodrug thereof, together with a pharmaceutically acceptable diluent, excipient or carrier, where such pharmaceutical composition is for administration to an individual suffering from a disorder or disease such as a cancer, or of another proliferative disorder or disease, including a cancer that can be treated by the inhibition of the activity of one or more cyclin dependent kinases, such as the inhibition of the activity of CDKl, CDK2, CDK4, CDK7, and/or CDK9.
• the invention relates to methods that involve contacting a cell, including a tumor cell, with an amount, such as a therapeutically effective amount, of the subject compound.
• these methods include the inhibition of the activity of one or more cyclin dependent kinases disclosed herein, such as a kinase selected from: CDKl, CDK2, CDK4, CDK7 and/or CDK9.
• the kinase inhibited is CDK9.
• the cell is contacted with the subject compound in vitro, while in an alternative embodiment the cell is included in a tumor or a cancer present within an individual, such as a mammal including a human.
• Such individual many suffer from a proliferative disorder or disease, including a cancer or a disorder that can be treated by the inhibition of the activity of one or more cyclin dependent kinases, such as the inhibition of the activity of CDKl, CDK2, CDK4, CDK7, and/or CDK9.
• a proliferative disorder or disease including a cancer or a disorder that can be treated by the inhibition of the activity of one or more cyclin dependent kinases, such as the inhibition of the activity of CDKl, CDK2, CDK4, CDK7, and/or CDK9.
• such disorder or disease is associated with the activity of CDK9.
• such disorder or disease is associated with the activity of CDKl, CDK2 and/or CDK4.
• said treatment leads to the inhibition of phosphorylation of a CDK substrate selected from: RNAPII, Rb and Eg-5; in cells.
• said inhibition of RNAPII phosphorylation is more pronounced than said inhibition of Rb and/or Eg-5 phosphorylation by a factor selected from: about 2-fold, about 5-fold, about 10-fold about 50- fold, and greater than 100-fold.
• tumors may be solid tumors, which are cancers of body tissues other than blood, bone marrow, or the lymphatic system.
• tumors may be hematological tumors, such as leukemia and lymphomas.
• Leukemia is a collective term for malignant diseases characterized by a proliferation of malignantly changed white blood cells. Diseases arising from lymphatic tissue are called lymphomas.
• Solid tumors may be selected from: liver cancer, stomach cancer, colon cancer, breast cancer, pancreas cancer, prostate cancer, skin cancer, renal cancer, bone cancer, thyroid cancer, skin cancer, including squamous cell carcinoma, esophagial cancer, kidney cancer, bladder cancer, gall cancer, cervical cancer, ovarian cancer, lung cancer (including bronchial, small and non-small-cell lung cancer), gastric cancer, and head and neck cancer.
• Hematological tumors may be leukemia, such as Acute Myelogenous Leukemia (AML), Acute Lymphoblastic Leukemia (ALL), Acute Lymphocytic Leukemia, Acute Acute myelogenous Leukemia (AML), Acute Lymphoblastic Leukemia (ALL), Acute Lymphocytic Leukemia, Acute Acute myelogenous Leukemia (AML), Acute Lymphoblastic Leukemia (ALL), Acute Lymphocytic Leukemia, Acute Acute myelogenous Leukemia, Acute Lymphoblastic Leukemia, Acute Lymphoblastic Leukemia, Acute Acute ALL of lymphoblastic Leukemia, Acute Acute ALL of lymphoblastic Leukemia, Acute Acute ALL of lymphoblastic Leukemia, Acute Acute ALL of lymphoblastic Leukemia, Acute Acute ALL of lymphoblastic Leukemia, Acute Acute ALL of lymphoblastic Leukemia, Acute Acute
• Leukemia Acute Promyelocytic Leukemia, Chronic Granulocytic Leukemia (CGL), Chronic
• CLL Chronic Lymphocytic Leukemia
• CML Chronic Myelogenous Leukemia
• Eosinophilic Leukemia Erythro leukemia, Extranodal Lymphoma, Follicular Lymphoma, Hairy Cell Leukemia, Monocytic Leukemia, Prolymphocytic Leukemia.
• Hematological tumors may also be lymphoma, such as B Cell Lymphomas, Burkitt Lymphoma, Cutaneous T Cell Lymphoma, High-Grade Lymphoma, Hodgkin's Lymphoma, Non-Hodgkin's Lymphoma, Low-grade Lymphoma, Lymphoblastic Lymphoma, Mantle Cell Lymphoma, Marginal Zone Lymphoma, Mucosa-Associated Lymphoid Tissue (MALT) Lymphomas, T Cell Lymphomas, peripheral T cell lymphoma, multiple myeloma, Essential Thrombocythemia, Hairy Cell Lymphoma, Extramedullary Myeloma, Granulocytic Sarcomae.
• lymphoma such as B Cell Lymphomas, Burkitt Lymphoma, Cutaneous T Cell Lymphoma, High-Grade Lymphoma, Hodgkin's Lymphoma, Non-Hodgkin's
• Hematological tumors may also be tumors of myeloid lineage, including acute and chronic myelogenous leukemias, myelodysplastic syndrome, and promyelocytic leukaemia.
• Tumors may also be of mesenchymal origin, such as fibrosarcoma and rhabdomyosarcoma.
• tumors may be tumors of the central and peripheral nervous system, such as astrocytoma, neuroblastoma, glioma, and schwannomas; and tumors may be other tumors, such as melanoma, seminoma, teratocarcinoma, osteosarcoma, xenoderoma pigmentosum, keratoctanthoma, thyroid follicular cancer, and Kaposi's sarcoma.
• Tumors that are resistant or refractory to treatment with other anti-cancer or antiproliferative agents may also benefit from treatment with the methods and pharmaceutical compositions of the present invention.
• the compound disclosed herein may also be useful in inhibiting tumor angiogenesis and metastasis.
• the compound of this invention, a pharmaceutically acceptable salt thereof, or a prodrug thereof may also be useful in combination (administered together or sequentially) with known anti-cancer treatments such as radiation therapy or with other anti-cancer, antiproliferative, cytostatic or cytotoxic agents.
• known anti-cancer treatments such as radiation therapy or with other anti-cancer, antiproliferative, cytostatic or cytotoxic agents.
• Other anti-cancer and anti-pro liferative agents which may be used in combination with the compound of the present invention include those decribed herein.
• the compound of the present invention, a pharmaceutically acceptable salt thereof, or a prodrug thereof may be further administered with any other anti-cancer and anti-proliferative agent disclosed herein.
• such combination products employ the compound of this invention, a pharmaceutically acceptable salt thereof, or a prodrug thereof, within the dosage range described herein and the other pharmaceutically active agent or treatment within its approved dosage range.
• Synergistic effects of drug combinations and effects on activity depending on order of administration are known in this field (see, e.g., J. Cell Sci., 108: 2897, 1995; Cancer Res. 57: 3375, 1997), and optimizing dosage and order of delivery will be within the skill of practitioner in the art utilizing the compound of the present invention.
• the compound described herein, a pharmaceutically acceptable salt thereof, or a prodrug thereof may also be administered sequentially with other anti-cancer or antiproliferative agents when a combination formulation is inappropriate.
• the invention is not limited in the sequence of administration; the compound described herein, a pharmaceutically acceptable salt thereof, or a prodrug thereof, may be administered either prior to, simultaneously with, or after administration of another anti-cancer or anti-proliferative agent. It is known, for example, that the cytotoxic activity of the cyclin-dependent kinase inhibitor flavopiridol is affected by the sequence of administration with anticancer agents (Cancer Research, 57, 3375, 1997).
• the invention provides a pharmaceutical package, wherein said package includes the compound of the present invention, a pharmaceutically acceptable salt thereof, or a prodrug thereof, or any pharmaceutical composition including such compound, pharmaceutically acceptable salt, or prodrug.
• the package comprises instructions which indicate that said pharmaceutical composition may be used for administration to an individual in need thereof, including a human, such as an individual suffering from a cancer or another proliferative disorder or disease.
• the pharmaceutical package includes the compound of the present invention, a pharmaceutically acceptable salt thereof, or a prodrug thereof, formulated together with another pharmaceutical ingredient such as an anti-cancer or anti-proliferative agent.
• the compound of the present invention, a pharmaceutically acceptable salt thereof, or a prodrug thereof, and the other pharmaceutical ingredient may be formulated together or separately and in individual dosage amounts.
• Other pharmaceutical ingredients that may be formulated together or separately with the compound of the present invention, a pharmaceutically acceptable salt thereof, or a prodrug thereof include but are not limited to other anti-cancer and anti-proliferative agents such as described above.
• the pharmaceutical package comprises instructions to administer such active ingredient to an individual in need thereof.
• the invention provides a pharmaceutical package for administration of a pharmaceutical composition to an individual suffering from a cancer, or from another proliferative disorder or disease, wherein said package includes at least the compound of the present invention, a pharmaceutically acceptable salt thereof, or a prodrug thereof.
• the pharmaceutical package comprises instructions to administer such pharmaceutical composition to an individual suffering from a cancer, or another proliferative disorder or disease.
• pharmaceutical package and “pharmaceutical pack” refer to any packaging system for storing and dispensing individual doses of medication for the treatment of an individual.
• the pharmaceutical package contains sufficient daily dosage units appropriate to the treatment period or in amounts which facilitate the individual's compliance with the regimen.
• the pharmaceutical pack comprises one or more vessels that include the active ingredient, e.g., the compound of the present invention.
• Such vessel can be a container such as a bottle, vial, syringe, or capsule, or may be a unit dosage form such as a pill.
• the active ingredient may be provided in the vessel in a pharmaceutically acceptable form or may be provided, for example, as a lyophilized powder.
• the pharmaceutical pack may further include a solvent to prepare the pharmaceutical composition including such active ingredient for administration.
• the pharmaceutical composition including such active ingredient may be already provided in a delivery device, such as a syringe, or a suitable delivery device may be included in the pack.
• the pharmaceutical package may comprise pills, liquids, gels, tablets, dragees or the pharmaceutical preparation in any other suitable form.
• the package may contain any number of daily pharmaceutical dosage units.
• the package may be of any shape, and the unit dosage forms may be arranged in any pattern, such as circular, triangular, trapezoid, hexagonal or other patterns.
• One or more of the doses or subunits may be indicated, for example to aid the doctor, pharmacist or patient, by identifying such dose or subunits, such as by employing color-coding, labels, printing, embossing, scorings or patterns.
• the pharmaceutical package may also comprise instructions for the individual to be administered, the doctor, the pharmacist or any other person related to the act of administering the pharmaceutical composition to such individual.
• Some embodiments comprise the administration of more than one active ingredient, including the compound as disclosed herein. Such administration may occur concurrently or sequentially.
• the active ingredients may be formulated together such that one administration delivers all components. Alternatively the active ingredients may be formulated separately.
• the pharmaceutical package may comprise the compound of the present invention and the other pharmaceutical ingredient in a single formulation, i.e., they are formulated together, or the compound of the present invention and the other pharmaceutical ingredient in individual formulations, i.e., they are formulated separately.
• Each formulation may comprise the compound of the present invention, a pharmaceutically acceptable salt thereof, or a prodrug thereof, and the other pharmaceutical ingredient(s) in individual dosage amounts (in approximately equal or unequal amounts).
• Administration of the compound of the present invention, a pharmaceutically acceptable salt thereof, or a prodrug thereof, and the other pharmaceutical ingredient(s) is typically such that a concentration results that is a therapeutically effective amount when in such combination.
• instructions means a product label, package insert and/or documents or other information describing relevant materials or methodologies pertaining to assembly, preparation or use of a kit or packaged pharmaceutical. These materials may include any combination of the following: background information, steps or procedures to follow for storage, preparation or administration, listing of components, indications for use, proposed dosages, warnings regarding possible side effects, instructions for administering the active ingredient(s), instructions in the case of overdose or ineffectiveness, technical support, and any other related documents. Instructions can be supplied in printed form, such as a package label or a package insert.
• a packaged pharmaceutical or a pharmaceutical composition can be inserted in a delivery carton or finished package, e.g., as a package insert, and the text of such may require approval by a competent regulatory authority such as the Food and Drug Administration (FDA) of the United States.
• FDA Food and Drug Administration
• instruction may also be stored in electronic form, e.g., on a computer- readable storage medium such as a computer-readable memory device, a centralized database, magnetic media such as hard disks, floppy disks, and magnetic tape; optical media such as compact discs, CD-ROMs and holographic devices; magneto -optical media such as floptical disks; and hardware devices that are specially configured to store and execute program code, such as application-specific integrated circuits (ASICs), programmable logic devices (PLDs) and ROM (read only memory) and RAM (random access memory) devices.
• Instructions may comprise a web address of an internet website from which more detailed instructions may be downloaded, or a recorded presentation. Instructions can contain one or multiple documents or future updates.
• the invention further relates to a method of synthesizing a compound of claim 1 or optionally a salt thereof, comprising the step of reacting a compound having a structure represented by formula (II) with the compound having the structure represented by formula
• X is -OEt.
• the invention further relates to a method of synthesizing a pharmaceutically acceptable salt of the present invention, comprising the step of reacting the compound of the present invention with an acid.
• the acid is hydrochloric acid.
• the acid is maleic acid.
• Example A The subject compound was invented as described in Example A, and can be synthesized as shown in Examples B.I to B.8.
• the surprising anti-pro liferative activities of the compound according to the present invention in xenograft tumor models are demonstrated in Examples Cl to C.5.
• Example D shows the potent inhibition of in-cell RNAPII phosphorylation by the subject compound.
• WO 03/033499, WO 2004/092139; and WO 2005/063765 disclose a number of specific l-phenyl-3-isopropyl-6-arylmethyl-pyrazolo[3,4-d]pyrimidin-4-ones, including, among other compounds, l-(2,6-dichlorophenyl)-6-(3-hydroxy-4-pyrrolidinomethyl- phenyl)methyl-3-isopropyl-pyrazolo[3,4-d]pyrimidin-4-one (Compound P, see above).
• Compound I showed a significantly more potent activity in different xenograft tumor models, including an A2780 xenograft model using two different dosing regimens compared to Compound P (see Example C);
• ADMET parameter of solubility about 390 ⁇ M at pH 7.4 for Compound I compared to about 210 ⁇ M for Compound P
• ADMET parameter of solubility about 390 ⁇ M at pH 7.4 for Compound I compared to about 210 ⁇ M for Compound P
• IC 50 values for Compound I ranging between about 800 nM to about 10 nM (compared to IC50 values for Compound P ranging between about 1.6 ⁇ M to about 16 nM) ;
• the assays used for determining the properties of the kinase inhibitors were performed in accordance with standard procedures well known to those skilled in the art. For example, assays for determining inhibitory activity on CDKs or in cellular assays are described in WO 00/218962, WO 2005/026129, and WO 2005/063765. Solubility assays at pH 7.4 were essentially performed as described by Avdeef, A. in Pharmacokinetic Optimization in Drug Research; Testa, B.; van de Waterbeemd, H.; Folkers, G.; Guy, R. Eds.; Wiley- VHC: Zurich, 2001; pp 305-326. PAMPA permeability assays at pH 7.4 were essentially performed as described in Kansy et al, J. Med. Chem. 41 : 1007-10, 1998.
• Example B Synthesis of 6-(3-Hydroxy-4-(pyrrolidinomethyl)phenylmethyl)-3- isopropyl-l-(3,5-dichloropyridin-4-yl)-pyrazolo[3,4-d]pyrimidin-4-one (Examples B.1- B.7).
• Examples B.1-B.8 show in detail the steps for the synthesis of l-(3,5-dichloropyridin-
• the reaction mixture was cooled to room temperature, and tert-butyl alcohol was removed under reduced pressure.
• Example C Activity of compounds in xenograft tumor models.
• the subject compound shows surprisingly significant anti-tumor activity in a number of in-vivo models of human cancers, and is surprisingly superior compared to a prior art compound (Compound P). This surprising activity was shown following oral administration of the compounds in a series of controlled murine xenograft experiments conducted as described below.
• Xenograft tumor-models 100 athymic nu/nu female mice (6-8 weeks old) from CRL were allowed to acclimate for 4 days.
• Cells for each respective tumor type human A2780 ovarian tumor cells (ATCC), HCTl 16 colon carcinoma cells (ATCC) and H460 NSCLC
• mice were cultured in RPMI 1640 medium (Gibco) supplied with 15mg/L of insulin, 10% FCS and 1% Pen/Strep. The 3 rd passage of cells with approximate 90% confluence was used for these experiments. Briefly, on Day 0, mice were inoculated with 0.1 ml (total 5xlO 6 cells) of the respective cell-type as a cell suspension (5OxIO 6 cells/ml) by subcutaneous injection into the fat pad area near the mammary gland under light anesthesia.
• RPMI 1640 medium Gibco
• mice were inoculated with 0.1 ml (total 5xlO 6 cells) of the respective cell-type as a cell suspension (5OxIO 6 cells/ml) by subcutaneous injection into the fat pad area near the mammary gland under light anesthesia.
• a 2OG gavage needle was used for the oral treatment. Formulation of each compound was as described in the results. The volume of administration was 0.2 ml per 20 g body weight except for the Cytoxan (cyclophosphamide) group (positive control) which was dosed i.p. as 0.1 ml per 20 g body weight.
• CR of tumors.
• PR is defined when the tumor size is 50% or less than the starting (day 1) size but greater than 0.0 mg for three consecutive measurements during the course of the study.
• CR occurs when there is no measurable tumor mass for three consecutive measurements.
• Cures are defined when CR are maintained until the completion of the experiment.
• LCK Log Cell Kill
• T the estimated time required for the treatment group of mice to reach 1000 mg tumor size
• C the estimated time for the control group tumors to reach 1000 mg in size
• Td the estimated Tumor Doubling time of the control group tumors during exponential growth
• 3.32 the number of doublings required for a population to increase 1-logio unit.
• Toxic deaths are defined as deaths caused by compound treatment and not by advanced disease state. A death is considered toxic if the animal dies within 2 weeks after the final compound treatment and the tumor size has not reached 1000 mg. Non-tumor related deaths after this point are recorded, but not considered toxic deaths.
• T k - T 1 is compared with the distribution of values ⁇ (b) 1 k ⁇ ⁇ l b) that are obtained if the animals are randomly permuted between the two groups many times.
• T k is the estimated time for the group k of mice to reach 1000 mg in tumor size. If T k - T 1 is larger than the 90 th percentile
• Example Cl Ovarian cancer model (A2780 cell-line) - twice-daily oral administration
• Compound P (as hydrochloric acid salt) was formulated in 30% PEG300/70% water
• Compound I (as hydrochloric acid salt) was formulated in 20% PEG300/80% D5W (5% dextrose water) and administered by oral gavage twice every day for 10 days (bidxlO), while the Cytoxan control which was administered i.p. in saline every two days over a period of 10 days (q2dx5):
• Compound I not only were partial and complete responses of tumor growth seen, but a number of individual mice were totally cured. That is, the subject compound (unlike the prior-art compound or even the positive control), was able to reduce the size of the tumor below a detectable level for the entire duration of the experiment.
• Ovarian cancer model (A2780 cell-line) - daily oral administration
• a comparative trial was designed having the protocol summarized in the following table, with the free-base equivalent dose indicated in square brackets.
• Compound I and Compound P were formulated as above, and were administered p.o. every day for 10 days (qdxlO).
• Mice in Group B were administered with a compound known as R547 (DePinto et al, MoI. Cancer Ther. 5: 2644-2658, 2006) formulated in 20%DMA/30% PEG 300/50% water.
• Compound I shows a general increase in anti-tumor activity, as estimated by LCK, for each dose as compared to the corresponding dose of Compound P. Pair-wise analysis of certain groups of mice by permutation test (see below) showed that Compound I showed a significantly increased activity over Compound P at the highest dosage, while the generally observed superiority of Compound I over Compound P could not be significantly concluded using this stringent test, which was not able to reject the null- hypothesis at this level of significance for the 2 lower dosages.
• Colon cancer model (HCTl 16 cell-line) - daily oral administration
• a comparative trial was designed having the protocol summarized in the following table, with the free-base equivalent dose indicated in square brackets.
• Compound P and Compound I were formulated as above, and were administrated p.o. every day for 10 days (qdxlO).
• Mice in Group B were administered R547 (formulated as a suspension in 6% Cremophor EL®/40% PEG300/54% Saline) p.o. every day for 10 days.
• Compound I shows a general increase in anti-tumor activity, as estimated by LCK, for each dose (other than the lowest dose) as compared to the corresponding dose of Compound P.
• Pair- wise analysis of certain groups of mice by permutation test showed that Compound I showed a significantly increased activity over Compound P at the highest dosage, while the general superiority of Compound I over Compound P could not be significantly concluded using this stringent test, which was not able to reject the null- hypothesis at this level of significance for the two lower dosages.
• Example C.5 Activity of the subject compound in in-vivo models of other human cancers.
• the subject compound shows surprising and unexpected activity in other models of human cancers. This surprising activity is shown following i.v. or p.o. administration of the compounds in controlled murine xenograft experiments that can be conducted as described above.
• Tumor types for testing include: PC3 (prostate) and A2780 (lung). Experiments are conducted as described above, except that the appropriate tumor cell-line is used, and study compounds can, in certain experiments, be administered intravenously (i.v.) and as formulated appropriately.
• Example D Potent inhibition of in-cell RNAPII phosphorylation by the subject compound
• the subject compound I demonstrated an previously unknown mechanism of cellular activity as follows: compound I showed a pronounced and consistently potent ability to inhibit the cellular phosphorylation of RNAPII (driven by CDK9 activity) across a panel of mammalian tumor cell-models, while in contrast the cellular phosphorylation of Eg-5 and Rb (driven by CDKl and CDK4/6 activity respectively) was both less strongly - and also far more variably - inhibited. Indeed, the cellular inhibition of CDK9-dependent RNAPII phosphorylation was better correlated to inhibition of antiproliferative activity by compound I, than to cellular inhibition of CDKl, or CDK4/6-dependend substrate phosphorylation.
• CDK-dependent phosphorylation of the following substrates was investigated in cancer cells following treatment with compound I and R547 (DePinto et al., MoI Cancer Ther. 5: 2644-2658; 2006): (i) CDKl -dependent Eg-5 phosphorylation on threonine 927 (Blangy et al., 1995, Cell, 83(7): 1159-1169); (ii) CDK4/6-specif ⁇ c phosphorylation of Rb on serine 780 (Zarkowska & Mittnacht, J Biol Chem. 272(19): 12738-12746 1997; Connell- Crowley et al., MoI Biol Cell.
• RNAPII RNA polymerase II
• MDA-MB-435 melanoma
• MDA-MB-453 breast, carcinoma
• RNAPII RNA polymerase II
• Asynchronous cells were treated with an appropriate concentration of test compound or
• RNAPII (N-20; Santa Cruz) as an expression control. Visualization was conducted using secondary Alexa680- or IR800-coupled antibodies (Molecular Probes) against mouse or rabbit Fc tails on the Odyssey-system (Licor). Specific bands were quantified and IC50 values of inhibition of RNAPII phosphorylation were estimated.
• Thr927phos CDKl -dependent Eg-5 phospohorylation on threonine 927 (Thr927phos).
• Thr927phos was investigated by arresting cells over night in G2/M phase with 25 ng/ml nocodazole (optimized final concentration) followed by treatment with DMSO as control or an appropriate concentration of test compound for 1 hour. Detached as well as adherent cells were collected and lysed with CLB-I lysis buffer, the lysate cleared by centrifugation and subjected to SDS-PAGE. The proteins were blotted on PVDF membranes and subsequently analyzed by antibodies against total Eg-5 (Becton Dickinson) and Eg-5 Thr927phos (Bio legend). Visualization and estimation of IC50 was conducted as above.
• CDK4/6-sepeci ⁇ c phosphorylation of Rb on serine 780 (Ser780phos).
• Asynchronous growing cells were treated with an appropriate concentration of test compound or DMSO for
• Proteins were extracted using the AllPrep RNA/Protein kit according to the manufacturer's recommendations (Qiagen, Germany). Rb was immunoprecipitated over night using AbO5 antibody (Oncogene Inc.). Proteins were separated by SDS-PAGE, blotted to
• PVDF membranes and analyzed with antibodies against total (sc-102; Santa Cruz) or Ser780- phosphorylated Rb proteins (Cell Signalling). Visualization and quantification was performed as above.
• IC 50 values for inhibition of substrate-phosphorylation for CDKl, CDK4/6 and CDK9 by compound I or R547 The following tables show IC 50 values for inhibition of substrate-phosphorylation for CDKl, CDK4/6 and CDK9 by compound I or R547.
• Compound I was a consistently potent inhibitor of CDK9-dependent phosphorylation of RNAPII, compared to the far less potent inhibition of such phosphorylation by R547. This distinction can be compared to the enzymatic IC50S of CDK9 activity as estimated in biochemical assays as described earlier, are between about 1 to 10 nM for both compound I and R547.
• compound I shows a higher degree of variability in its potency of CDKl- dependent phosphorylation of Eg-5, compared to the relatively consistent IC50s in the same assays with R547. This consistency shows that the assays are reliable per se.
• R547 is a more potent CDKl inhibitor than compound I (IC 50 S for CDKl are between about 1 and 10 nM and between about 10 and 50 nM, respectively).
• R547 is a moderately more potent CDK4 and CDK6 inhibitor (IC 50 S for CDK4 and CDK6 in both cases about 10 nM) than compound I (IC 50 S for CDK4 and CDK6 about 50 nM, and between about 10 and 50 nM, respectively).
• experiments may also include the elucidation and/or determination of the mechanism of action of the subject compound, the target or target profile of the subject compound, and other characteristics of the subject compound, such as the binding affinity of the compound to the target(s) or the binding site of the compound on the target(s) and pharmacokinetic properties.
• Such experiments may also include molecular modeling of the drug-target interaction and the identification of metabolites formed after administration, or identification of the active agent.
• the active ingredient and/or pharmaceutical composition including such active ingredient that shows the most appropriate results, including results for inhibition of cell proliferation, spectrum across various tumor cell lines, inhibition of tumor growth or tumor reduction data and/or animal-survival data, and/or other features, including ADMET, pharmacokinetic and pharmacodynamic properties, may be chosen to enter further experiments. Such experiments may include, for example, therapeutic profiling and toxicology in animals, phase I clinical trials in humans and other clinical trails.

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