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DK2944695T3 - Hidtil ukendte plante-ekspressionskonstrukter - Google Patents

Hidtil ukendte plante-ekspressionskonstrukter Download PDF

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Publication number
DK2944695T3
DK2944695T3 DK15169140.9T DK15169140T DK2944695T3 DK 2944695 T3 DK2944695 T3 DK 2944695T3 DK 15169140 T DK15169140 T DK 15169140T DK 2944695 T3 DK2944695 T3 DK 2944695T3
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plant
seq
promoter
gene
sequence
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DK15169140.9T
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Karen L Fincher
Stanislaw Flasinski
Jack Q Wilkinson
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Monsanto Technology Llc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
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    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • Biomedical Technology (AREA)
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  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
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  • Pest Control & Pesticides (AREA)
  • Medicinal Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Reproductive Health (AREA)
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  • Agronomy & Crop Science (AREA)
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (15)

1. Fremgangsmåde til fremstilling af en plante, der eksprimerer en strukturel gensekvens, hvilken fremgangsmåde omfatter: (1) tilvejebringelse af et DNA-konstrukt omfattende en promoter, der er funktionel i en plantecelle, hvilken promoter omfatter SEQ ID NO:28; en strukturel gensekvens; og en 3'-ikke-translateret region, der har funktionen at bevirke tilførsel af polyadenylerede nukleotider til 3'-enden af RNA-sekvensen; hvor den strukturelle gensekvens er funktionsmæssigt koblet til promoteren og den 3'-ikke-translaterede region, og promoteren er heterolog i forhold til den strukturelle gensekvens; (2) indføring af DNA-konstruktet i en plantecelle; og (3) regenerering af plantecellen til fremstilling af en plante, således at den strukturelle gensekvens kan eksprimeres i planten.
2. Fremgangsmåde ifølge krav 1, hvor den strukturelle gensekvens er et her-bicidtolerancegen.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor herbicidtolerancegenet er et glyphosattolerancegen.
4. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, hvor glypho-sattolerancegenet er et 5-enolpyruvylshikimat-3-phosphat-(EPSP)-syntasegen eller et glyphosatoxidoreduktasegen.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 4, hvor glypho-sattolerancegenet er et aroA:CP4-gen.
6. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 5, hvor DNA-konstruktet giver plantecellen tolerance over for en vandig glyphosatformule-ring omfattende mindst 50 gram syreækvivalent pr. liter glyphosat.
7. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 6, hvor DNA-konstruktet giver plantecellen tolerance over for en vandig glyphosatformule- ring omfattende mindst 300 gram syreækvivalent pr. liter glyphosat.
8. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 7, hvor planten er alfalfa, broccoli, kål, raps, blomkål, majs, bomuld, tranebær, agurk, salat, ært, poppel, fyr, kartoffel, løg, ris, hindbær, sojabønne, sukkerrør, sukkerroe, solsikke, tomat eller hvede.
9. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 8, hvor planten er bomuld, tomat, rasp, sojabønne eller solsikke.
10. Transgen afgrødeplante omfattende et DNA-konstrukt omfattende den kimæriske promoter-DNA-sekvens med SEQ ID NO:28 og en 3'-ikke-translateret region, der har funktionen i planter at bevirke tilførsel af polyade-nylerede nukleotider til 3'-enden af RNA-sekvensen; hvor en strukturel gensekvens er funktionsmæssigt koblet til den kimæriske promoter og den 3'-ikke-translaterede region, og den kimæriske promoter-DNA-sekvens er heterolog i forhold til den strukturelle gensekvens.
11. Transgen afgrødeplante ifølge krav 10, hvor afgrødeplanten er en enkim-bladet eller en tokimbladet afgrødeart.
12. Transgen afgrødeplante ifølge krav 10 eller 11, hvor planten er alfalfa, broccoli, kål, raps, blomkål, majs, bomuld, tranebær, agurk, salat, ært, poppel, fyr, kartoffel, løg, ris, hindbær, sojabønne, sukkerrør, sukkerroe, solsikke, tomat eller hvede.
13. Transgen afgrødeplante ifølge et hvilket som helst af kravene 10 til 12, hvor det strukturelle gen er et glyphosattolerancegen.
14. Transgen afgrødeplante ifølge et hvilket som helst af kravene 10 til 13, hvor glyphosattolerancegenet er et EPSP-syntasegen eller et glyphosatoxi-doreduktasegen.
15. Transgen afgrødeplante ifølge et hvilket som helst af kravene 10 til 14, hvor glyphosattolerancegenet er et aroA:CP4-gen.
DK15169140.9T 1999-12-16 2000-12-12 Hidtil ukendte plante-ekspressionskonstrukter DK2944695T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17117399P 1999-12-16 1999-12-16
EP05111039.3A EP1632576B1 (en) 1999-12-16 2000-12-12 Novel plant expression constructs

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DK2944695T3 true DK2944695T3 (da) 2017-05-15

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DK15169140.9T DK2944695T3 (da) 1999-12-16 2000-12-12 Hidtil ukendte plante-ekspressionskonstrukter
DK00984233.7T DK1240340T3 (da) 1999-12-16 2000-12-12 Dna-konstrukter til ekspression af heterologe polypeptider i planter

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US (12) US6462258B1 (da)
EP (5) EP1240340B1 (da)
JP (1) JP5111706B2 (da)
CN (5) CN101311267B (da)
AR (6) AR026994A1 (da)
AT (1) ATE555206T1 (da)
AU (1) AU782697B2 (da)
BR (2) BR0016460B1 (da)
CA (5) CA2839066A1 (da)
DK (2) DK2944695T3 (da)
ES (3) ES2638112T3 (da)
ID (1) ID28665A (da)
MX (1) MXPA02006021A (da)
PT (2) PT1240340E (da)
SI (1) SI1240340T1 (da)
TR (2) TR200702139T2 (da)
WO (1) WO2001044457A2 (da)
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