CN221580650U - Microfluidic chip and microfluidic device - Google Patents

Abstract

The utility model provides a microfluidic chip and a microfluidic device, which relate to the technical field of microfluidics and comprise a sample feeding well, a mixer, a sample discharging well and a micro-channel, wherein one end of the mixer is connected with the sample discharging well, and the other end of the mixer is respectively connected with all the micro-channels; the microfluidic chip further comprises a plurality of cleaning passages, the cleaning passages are arranged in one-to-one correspondence with the micro flow channels, one end of each cleaning passage is connected with each micro flow channel, and the other end of each cleaning passage is selectively communicated with the outside; through setting up in the cleaning passageway of microchannel intercommunication for the user can wash the microfluidic chip through the inside injection washing liquid of cleaning passageway to microchannel and microfluidic chip, avoids the risk of sample pollution and cross contamination in the microfluidic chip used repeatedly, has solved the microchannel of microfluidic chip among the prior art and has all been disposable design use, has the technical problem that washs difficultly, unable used repeatedly, has reduced the experimental cost of nano-drug research and development.

Description

Microfluidic chip and microfluidic device

Technical Field

The utility model relates to the technical field of microfluidics, in particular to a microfluidic chip and a microfluidic device.

Background

Repeated experimental demonstration in the drug screening process at the initial stage of drug development is a necessary stage for drug development of nano-drug users. It is often necessary during experiments to use microfluidic mixing techniques, which utilize interactions between fluids to achieve mixing by introducing two or more different liquids simultaneously into a microchannel, while techniques that use microchannels (tens to hundreds of microns in size) to process or manipulate microscopic fluids are also known as microfluidic techniques.

At present, micro liter synthesis of nano-drugs is completed by using a micro-fluidic technology, and is generally realized by a micro-fluidic device. And a microfluidic chip is arranged in the microfluidic device, the to-be-fused solution with corresponding proportion is respectively injected into the microfluidic chip, and the solution is synthesized through a micro-channel in the microfluidic chip to obtain the synthesized drug. The micro-fluidic chip is usually classified by the mixing amount, such as micro-upgrade mixed micro-fluidic chip and milliliter mixed micro-fluidic chip, and the micro-flow channel of the micro-liter mixed micro-fluidic chip is far smaller than that of the milliliter mixed micro-fluidic chip.

However, the micro-channels of the conventional micro-upgrade mixed micro-fluidic chip are all designed and used at one time, and the technical problems that the micro-channels are difficult to clean and cannot be reused exist.

Disclosure of utility model

The utility model aims to provide a microfluidic chip and a microfluidic device, which are used for relieving the technical problems that micro-channels of micro-liter-level mixed microfluidic chips in the prior art are all designed to be disposable, are difficult to clean and cannot be reused.

The utility model provides a microfluidic chip, which comprises a plurality of sample feeding wells, mixers, sample discharging wells and micro flow channels which are connected with the sample feeding wells in a one-to-one correspondence manner, wherein one end of each mixer is connected with each sample discharging well, and the other end of each mixer is respectively connected with all the micro flow channels;

The microfluidic chip further comprises a plurality of cleaning passages which are arranged in one-to-one correspondence with the micro flow channels, one end of each cleaning passage is connected with each micro flow channel, and the other end of each cleaning passage is selectively communicated with the outside.

Further, the microfluidic chip further comprises a buffer well, the buffer well is arranged at one end of the micro-channel close to the mixer, and the cleaning passage is connected with the bottom of the buffer well.

Further, the microfluidic chip comprises a chip micro-channel plate and a chip bonding plate thermally pressed and bonded with the chip micro-channel plate;

the sample feeding well and the sample discharging well are arranged at the top of the chip micro-channel plate, the micro-channel and the mixer are arranged at the bottom of the chip micro-channel plate, and the cleaning passage and the buffer well are arranged on the chip bonding plate.

Further, the die bonding plate further comprises a cleaning film, and the cleaning film covers the bottom of the die bonding plate to seal the cleaning passage.

Further, the mixer comprises a mixing ring;

The mixing rings are sequentially connected in series to form the mixer in a surrounding manner.

Further, the mixing ring is elliptical.

Further, the microfluidic chip further comprises a chip buckle cover, the chip buckle cover is buckled on the chip micro-channel plate, an air inlet hole and a pressure relief hole are formed in the chip buckle cover, the air inlet hole is communicated with all the sample feeding wells, and the pressure relief hole is communicated with the sample discharging wells.

Further, the bottom of chip buckle closure is provided with the well seal district that goes up, the air inlet set up in the top of well seal district that goes up, go up the bottom of well seal district respectively with go up the well intercommunication, be provided with the sealing ring in the well seal district that goes up.

Further, the bottom of chip buckle closure is provided with out the appearance well sealing area, go out the bottom of appearance well sealing area with go out appearance well intercommunication, a plurality of the pressure release hole evenly set up in go out the top of appearance well sealing area.

In another aspect of the present utility model, a microfluidic device is provided, including the microfluidic chip described above.

The beneficial effects are that:

The utility model provides a microfluidic chip, which comprises a plurality of sample feeding wells, a mixer, sample discharging wells and micro flow channels which are connected with the sample feeding wells in a one-to-one correspondence manner, wherein one end of the mixer is connected with the sample discharging wells, and the other end of the mixer is respectively connected with all the micro flow channels; the microfluidic chip further comprises a plurality of cleaning passages, the cleaning passages are arranged in one-to-one correspondence with the micro flow channels, one end of each cleaning passage is connected with each micro flow channel, and the other end of each cleaning passage is selectively communicated with the outside; through setting up in the cleaning passageway of microchannel intercommunication for the user can wash the microfluidic chip through cleaning passageway to the inside injection washing liquid of microchannel and microfluidic chip, avoids the risk of sample pollution and cross contamination in the microfluidic chip used repeatedly, so that microfluidic chip can be used repeatedly, has solved the microchannel of the mixed microfluidic chip of microliter level among the prior art and has all been disposable design use, has the technical problem that washs difficultly, unable used repeatedly, has reduced the experimental cost of nano-drug research and development.

The utility model also provides a microfluidic device comprising the microfluidic chip, and the microfluidic device has all advantages compared with the prior art, and is not repeated herein.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

Fig. 1 is a schematic diagram of the overall structure of a microfluidic chip according to an embodiment of the present utility model;

fig. 2 is a top view of an overall structure of a microfluidic chip according to an embodiment of the present utility model;

FIG. 3 is a schematic bonding diagram of a chip micro flow channel plate and a chip bonding plate in a micro flow control chip according to an embodiment of the present utility model;

Fig. 4 is a schematic diagram illustrating connection of a buffer well and a cleaning channel in a microfluidic chip according to an embodiment of the present utility model;

Fig. 5 is a schematic structural diagram of a buffer well and a mixer of a microfluidic chip according to an embodiment of the present utility model;

fig. 6 is a schematic structural diagram of a chip cover of a microfluidic chip according to an embodiment of the present utility model.

Icon:

100. A chip micro-channel plate; 110. loading a sample well; 120. sampling well; 130. a microchannel; 140. a mixer; 141. a mixing ring;

200. A die bonding plate; 210. cleaning the passage; 220. a buffer well;

300. The chip is covered; 310. an air inlet hole; 320. a pressure relief hole; 330. loading a sample well sealing area; 340. and (5) discharging a sealing area of the well.

Detailed Description

The utility model is described in further detail below with reference to the drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the utility model and are not limiting thereof. It should be further noted that, for convenience of description, only some, but not all of the structures related to the present utility model are shown in the drawings.

In the description of the present utility model, unless explicitly stated and limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

In the present utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.

The utility model will now be described in further detail with reference to specific examples thereof in connection with the accompanying drawings.

As shown in fig. 1 and fig. 2, the embodiment of the present utility model provides a microfluidic chip, which includes a plurality of sample wells 110, a mixer 140, sample wells 120, and micro-channels 130 connected to the sample wells 110 in a one-to-one correspondence manner, wherein one end of the mixer 140 is connected to the sample wells 120, and the other end of the mixer 140 is respectively connected to all the micro-channels 130; the microfluidic chip further comprises a cleaning passage 210, the cleaning passages 210 are arranged in one-to-one correspondence with the micro flow channels 130, one end of each cleaning passage 210 is connected with the micro flow channel 130, and the other end of each cleaning passage 210 is selectively communicated with the outside.

That is, according to the microfluidic chip provided by the embodiment of the utility model, through the cleaning channel 210 which is arranged in the communication of the micro-flow channel 130, a user can inject cleaning liquid into the micro-flow channel 130 and the interior of the microfluidic chip through the cleaning channel 210 to clean the microfluidic chip, so that the microfluidic chip can be reused, the technical problems that the micro-flow channel 130 of the microfluidic chip is designed and used once in the prior art, the cleaning is difficult and the micro-flow channel cannot be reused are solved, and the experimental cost for developing nano medicines is reduced.

Specifically, in this embodiment the upper sample well 110 is provided with two, both of which are at 3: 1. Two sample wells 110 are provided on top of the microfluidic chip at the same level. The sample well 110 is arranged in a funnel shape, and the top of the sample well is arranged at a wide mouth. The two micro-channels 130 are also provided, one ends of the two micro-channels 130 are respectively connected with the bottoms of the two sample wells 110, and the other ends of the two micro-channels 130 are connected with the mixer 140. The aqueous phase solution and the organic phase solution to be fused can be placed in the two sample wells 110 through the pipettes, respectively, and the two solutions in the sample wells 110 are pressed into the micro flow channels 130 by external pressure, and mixed in the mixer 140. The other end of the mixer 140 is connected to the well 120, and the mixed solution is further pressed into the well 120 to complete the synthesis of one microliter of the solution. The cleaning passage 210 is communicated with the bottom of the micro flow channel 130, and external cleaning liquid can be injected into the micro flow channel 130 through the cleaning passage 210, so that the whole micro flow channel 130 is cleaned, and the risks of sample pollution and cross contamination in the repeated use process of the micro flow control chip are avoided.

As shown in fig. 1 and fig. 4, the microfluidic chip provided in the embodiment of the present utility model further includes a buffer well 220, the buffer well 220 is disposed at one end of the micro flow channel 130 near the mixer 140, and the cleaning channel 210 is connected to the bottom of the buffer well 220.

Specifically, the buffer well 220 is formed in an approximately cylindrical shape as a whole, and is provided with an arc surface at the bottom thereof, the top thereof communicates with the micro flow channel 130, and the bottom thereof has an opening communicating with the cleaning passage 210. The buffer well 220 is disposed at the end of the micro flow channel 130 and is disposed close to the mixer 140. When the two solutions in the sample well 110 are pressurized into the micro flow channel 130, they need to enter the buffer well 220 before entering the mixer 140. By providing the mixer 140, the particle size and PDI (Polymer dispersity index polymer dispersibility index) value of the final lipid nanoparticle can be prevented from being affected by capillary action, advanced entering the mixer 140, or mutual channeling of the two-phase solution. In this embodiment, the cleaning passage 210 is configured as a stepped cleaning hole, and the stepped aperture of the cleaning hole is matched with the external dimension of the injection head of the pipette, so that the injection head of the pipette can extend into the cleaning passage 210 to inject the cleaning liquid into the cleaning passage 210.

As shown in fig. 2 and 3, further, the microfluidic chip includes a chip micro flow channel plate 100 and a chip bonding plate 200 thermally compression bonded to the chip micro flow channel plate 100; the loading well 110 and the unloading well 120 are disposed at the top of the chip micro flow channel plate 100, the micro flow channel 130 and the mixer 140 are disposed at the bottom of the chip micro flow channel plate 100, and the cleaning passage 210 and the buffer well 220 are disposed at the chip bonding plate 200.

Specifically, the chip micro flow channel plate 100 is made of thermoplastic or thermo-elastomer, and the material may be Polycarbonate (PC), polystyrene (PP), cyclic olefin homopolymer (COP), cyclic Olefin Copolymer (COC), polymethyl methacrylate (PMMA), or Polydimethylsiloxane (PDMS). The sample well 110 is disposed at the top of the chip micro flow channel plate 100, the top opening of the sample well 110 is disposed, and the sample well 110 can be processed on the chip micro flow channel plate 100 by CNC precision machining and laser etching. The micro flow channel 130 and the mixer 140 are disposed at the bottom of the chip micro flow channel plate 100, and the micro flow channel 130 groove and the mixer 140 groove are etched at the bottom of the micro flow channel 130 by means of laser etching. The die bonding plate 200 is provided with the same material as the die micro flow channel plate 100. The buffer well 220 is disposed on top of the chip bonding plate 200 by CNC precision machining in combination with laser etching, and corresponds to a preset position on the chip micro flow channel plate 100. While purge via 210 is disposed in die bond plate 200 on top of buffer well 220 and extends through die bond plate 200. After the chip bonding plate 200 and the chip microfluidic plate are completely etched, the chip bonding plate and the chip microfluidic plate can be cleaned, dried and then placed into a pressurized heating die. The die bonding plate 200 is thermally bonded to the die microfluidic plate by adjusting appropriate thermal bonding parameters, such as pressurization pressure, temperature rise rate, constant temperature time, temperature reduction rate, and the like. The chip micro flow channel plate 100 and the chip bonding plate 200 are integrated into a chip body by thermocompression bonding. The bonding strength of the chip main body bonded by hot pressing is high, no liquid leakage can be repeatedly used under the pressure of 4-5bar, the pressure resistance is high, and the service life of the microfluidic chip is effectively prolonged. Meanwhile, the micro flow channel 130 obtained through thermal compression bonding has the good properties of small section deformation, good flow channel tightness and high flow channel pressure resistance, so that the micro flow control chip has good micro liter synthesis effect.

Preferably, the die bonding plate 200 further includes a cleaning film covering the bottom of the die bonding plate 200 to seal the cleaning path 210.

Specifically, a cleaning film is attached to the bottom of the die bonding plate 200, sealing the cleaning path 210. When the microfluidic chip needs to be cleaned, the cleaning film can be torn off, so that the injection head of the liquid dispenser extends into the cleaning passage 210, and the cleaning liquid is injected into the microfluidic chip to clean the microfluidic chip. After the cleaning is finished, only a new cleaning film is needed to be stuck. The cleaning channel 210 is sealed by the cleaning membrane, so that the operation is convenient and fast, the cost is low, and the cleaning channel 210 can be effectively prevented from being contacted with the outside, so that the interior of the micro-channel 130 is polluted.

As shown in fig. 5, the mixer 140 provided by the embodiment of the present utility model includes a mixing ring 141; a plurality of mixing rings 141 are connected in series in sequence to enclose the mixer 140.

Specifically, in the present embodiment, five mixing rings 141 are provided, the mixing rings 141 are annular channels, and the five mixing rings 141 are sequentially connected in series to form the mixer 140. The mixing rings 141 at one end thereof are respectively connected to the two buffer wells 220 through flow channels. The mixing ring 141 at the other end is connected to the sample well 120. By providing the mixing rings 141 in series, it is possible to ensure that the dynamic shear force when the sample flows into the mixer 140 is small, and a stable laminar flow effect is easily obtained, thereby achieving a better synthesis effect.

Preferably, the mixing ring 141 is oval.

Specifically, in the present embodiment, the major axis of the outer contour ellipse of the mixing ring 141 is 1mm and the minor axis thereof is 0.5mm, and the inner contour ellipse of the mixing ring 141 is 0.8mm and the minor axis thereof is 0.3mm, so that the entire mixing ring 141 is elliptical. By arranging the mixing ring 141 in an elliptical shape, the dynamic shear force when the sample flows into the mixer 140 is further reduced, so that a more stable laminar flow effect is obtained, and the synthesis effect of the two solutions is ensured.

As shown in fig. 1 and 6, further, the microfluidic chip further includes a chip buckle cover 300, the chip buckle cover 300 is buckled on the chip micro-fluidic channel plate 100, an air inlet 310 and a pressure release hole 320 are provided on the chip buckle cover 300, the air inlet 310 is communicated with all the sample wells 110, and the pressure release hole 320 is communicated with the sample well 120.

Specifically, the chip clasp cover 300 is provided with a food grade sealing material such as Ethylene Propylene Diene Monomer (EPDM), silicone rubber, or Polytetrafluoroethylene (PTFE). An air intake hole 310 is provided at the top of the chip clasp cover 300 and communicates with the sample well 110. A pressure relief hole 320 is provided at the top of the sample well 120 and communicates with the sample well 120. Therefore, the solution in the two sample wells 110 can be simultaneously pumped into the micro flow channel 130 by inflating the air inlet 310, and the air in the micro flow channel 130 can enter the sample well 120 through the mixer 140 and be discharged from the pressure release hole 320, so as to ensure the air pressure in the chip main body to be stable.

Preferably, the bottom of the chip buckle cover 300 is provided with a sample well sealing area 330, an air inlet is arranged at the top of the sample well sealing area 330, the bottom of the sample well sealing area 330 is respectively communicated with the sample well 110, and a sealing ring is arranged in the sample well sealing area 330.

Specifically, the sample well sealing region 330 is disposed at the bottom of the chip clasp cap 300, which is V-shaped overall. The air inlet is disposed in the middle of the well seal area 330, and the wells 110 are disposed at both sides of the well seal area 330, respectively. The sealing ring is fixed in the sample well sealing area 330 to match the compression deformation of the chip buckle cover 300 to realize the sealing of the air inlet outlet, and the integral sealing effect of the microfluidic chip is ensured.

Further, the bottom of the chip buckle cover 300 is provided with a sample well sealing area 340, the bottom of the sample well sealing area 340 is communicated with the sample well 120, and a plurality of pressure relief holes 320 are uniformly formed at the top of the sample well sealing area 340.

Specifically, the pattern of the well seal 340 is circular and is positioned coaxially with the well 120. The pressure release holes 320 are uniformly formed in the top of the sample well sealing area 340 along the circumferential direction of the sample well sealing area 340, so that the pressure release effect of the sample well sealing area 340 is improved, and when the air inlet 310 is inflated, the air in the micro-channel 130 can be rapidly discharged through the pressure release holes 320 in the sample well sealing area 340, so that the guarantee is provided for the microlitre synthesis of the solution.

The utility model also provides a microfluidic device comprising the microfluidic chip. Compared with the prior art, the microfluidic device has all advantages, and is not repeated here.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and are not limiting; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced with equivalents; such modifications and substitutions do not depart from the spirit of the technical solutions according to the embodiments of the present utility model.

Claims (10)

1. The microfluidic chip is characterized by comprising a plurality of sampling wells (110), a mixer (140), a sampling well (120) and micro-channels (130) which are connected with the sampling wells (110) in a one-to-one correspondence manner, wherein one end of the mixer (140) is connected with the sampling well (120), and the other end of the mixer (140) is respectively connected with all the micro-channels (130);

The microfluidic chip further comprises a cleaning passage (210), a plurality of cleaning passages (210) are arranged in one-to-one correspondence with the micro flow channels (130), one end of each cleaning passage (210) is connected with each micro flow channel (130), and the other end of each cleaning passage (210) is selectively communicated with the outside.

2. The microfluidic chip according to claim 1, further comprising a buffer well (220), wherein the buffer well (220) is disposed at an end of the micro flow channel (130) close to the mixer (140), and wherein the cleaning channel (210) is connected to a bottom of the buffer well (220).

3. The microfluidic chip according to claim 2, wherein the microfluidic chip comprises a chip micro flow channel plate (100) and a chip bonding plate (200) thermocompression bonded to the chip micro flow channel plate (100);

The sample loading well (110) and the sample unloading well (120) are arranged at the top of the chip micro-channel plate (100), the micro-channel (130) and the mixer (140) are arranged at the bottom of the chip micro-channel plate (100), and the cleaning passage (210) and the buffer well (220) are arranged at the chip bonding plate (200).

4. The microfluidic chip of claim 3, wherein the die bond plate (200) further comprises a cleaning film covering a bottom of the die bond plate (200) to seal the cleaning channel (210).

5. The microfluidic chip according to any one of claims 1-4, wherein the mixer (140) comprises a mixing ring (141);

The mixing rings (141) are sequentially connected in series to enclose the mixer (140).

6. The microfluidic chip according to claim 5, wherein the mixing ring (141) is elliptical.

7. A microfluidic chip according to claim 3, further comprising a chip cover (300), wherein the chip cover (300) is fastened to the chip micro flow channel plate (100), an air inlet hole (310) and a pressure release hole (320) are provided on the chip cover (300), the air inlet hole (310) is communicated with all the sample loading wells (110), and the pressure release hole (320) is communicated with the sample unloading well (120).

8. The microfluidic chip according to claim 7, wherein a sample well sealing area (330) is arranged at the bottom of the chip fastening cover (300), the air inlet hole (310) is arranged at the top of the sample well sealing area (330), the bottom of the sample well sealing area (330) is respectively communicated with the sample well (110), and a sealing ring is arranged in the sample well sealing area (330).

9. The microfluidic chip according to claim 7, wherein a sample well sealing area (340) is provided at the bottom of the chip cover (300), the bottom of the sample well sealing area (340) is communicated with the sample well (120), and a plurality of pressure relief holes (320) are uniformly provided at the top of the sample well sealing area (340).

10. A microfluidic device comprising a microfluidic chip according to any one of claims 1-9.