CN210613738U

CN210613738U - Detection chip and detection system

Info

Publication number: CN210613738U
Application number: CN201920980362.XU
Authority: CN
Inventors: 申晓贺; 袁春根; 胡立教; 崔皓辰
Original assignee: BOE Technology Group Co Ltd; Beijing BOE Health Technology Co Ld
Current assignee: BOE Technology Group Co Ltd; Beijing BOE Health Technology Co Ld
Priority date: 2019-06-26
Filing date: 2019-06-26
Publication date: 2020-05-26
Anticipated expiration: 2029-06-26

Abstract

A detection chip and a detection system, the detection chip comprises a sample injection structure, a sample detection structure and a sample filtration structure. The sample injection structure is used for injecting a detected sample, the sample detection structure is used for enabling the detected sample to be detected, and the sample filtering structure is arranged between the sample injection structure and the sample detection structure and is respectively communicated with the sample injection structure and the sample detection structure so as to filter the injected detected sample in a lateral chromatography mode and transmit the filtered detected sample to the sample detection structure. The detection chip can enable a detected sample to be purer through the sample filtering structure, so that the detection result is more accurate, and the detection chip is favorably thinned.

Description

Detection chip and detection system

Technical Field

The embodiment of the disclosure relates to a detection chip and a detection system.

Background

The micro-fluidic chip technology integrates basic operation units related to sample preparation, reaction, separation, detection and the like in the fields of biology, chemistry, medicine and the like into a chip with a micro-channel with a micron scale, and automatically completes the whole process of reaction and analysis. The chip used in this process is called a microfluidic chip, and may also be called a Lab-on-a-chip (Lab-on-a-chip). The microfluidic chip technology has the advantages of less sample consumption, high analysis speed, convenience for manufacturing a portable instrument, suitability for real-time and on-site analysis and the like, and is widely applied to various fields of biology, chemistry, medicine and the like.

SUMMERY OF THE UTILITY MODEL

At least one embodiment of the present disclosure provides a detection chip including a sample injection structure, a sample detection structure, and a sample filtration structure. The sample injection structure is used for injecting a detected sample, the sample detection structure is used for enabling the detected sample to be detected, and the sample filtering structure is arranged between the sample injection structure and the sample detection structure and is respectively communicated with the sample injection structure and the sample detection structure so as to filter the injected detected sample in a lateral chromatography mode and transmit the filtered detected sample to the sample detection structure.

For example, at least one embodiment of the present disclosure provides a detection chip, wherein the sample filtering structure includes a filtering layer configured to receive the detected sample from the sample injection structure at a first side, filter the detected sample in a plane along which the filtering layer is located, and output the filtered detected sample at a second side opposite to the first side.

For example, at least one embodiment of the present disclosure provides a detection chip, wherein the sample injection structure and the sample filtration structure are connected by a first channel, and the sample filtration structure and the sample detection structure are connected by a second channel.

For example, at least one embodiment of the disclosure provides a detection chip, wherein, on the first side of the filter layer, the first channel at least partially overlaps and interfaces with the filter layer in a direction perpendicular to a plane of the filter layer, so as to inject the detected sample from the sample injection structure into the filter layer for filtering.

For example, at least one embodiment of the present disclosure provides a detection chip, wherein, on the second side of the filter layer, the second channel at least partially overlaps and interfaces with the filter layer in a direction perpendicular to a plane of the filter layer, so as to receive the filtered sample to be detected.

For example, in the detection chip provided in at least one embodiment of the present disclosure, in a direction perpendicular to a plane of the filter layer, the first channel and the second channel are respectively connected to different layer surfaces or the same layer surface of the filter layer.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample filtering structure further includes a filter chamber for accommodating the filter layer, the filter chamber includes a first opening and a second opening, the first opening is used for inputting the detected sample, the second opening is used for outputting the filtered detected sample, wherein the second opening is connected to the first end of the second channel, and the sample detection structure is connected to the second end of the second channel.

For example, the detection chip provided by at least one embodiment of the present disclosure further includes a first substrate and an adhesive layer, wherein the filter cavity is disposed on the first substrate, and the adhesive layer is attached to the surface of the first substrate and fixes the filter layer in the filter cavity.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the first channel and the second channel are disposed in the first substrate.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample injection structure and the sample detection structure are disposed on the first substrate.

For example, at least one embodiment of the present disclosure provides a detection chip further including a second substrate, wherein the second substrate is stacked on the first substrate and bonded by the adhesive layer, the adhesive layer defines the first opening and the second opening of the filter cavity, and the first channel and the second channel are formed in the second substrate and respectively communicate with the first opening and the second opening.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample injection structure and the sample detection structure are disposed on the second substrate.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample detection structure includes a plurality of detection units and a plurality of first detection flow channels, and at least one of the detection units is connected to the sample filtration structure through a corresponding first detection flow channel.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the width of the first detection flow channel is 0.1mm to 1mm, and the depth is 0.1mm to 1 mm.

For example, at least one embodiment of the present disclosure provides a detection chip, wherein at least a portion of the first detection flow channel extends in a curved manner.

For example, in the detection chip provided by at least one embodiment of the present disclosure, at least a portion of the curved extension of the first detection flow channel is in a zigzag shape or an S shape.

For example, in the detection chip provided by at least one embodiment of the present disclosure, at least a portion of the curved extension of the first detection flow channel includes 2 to 20 bends, and a length of the flow channel between two adjacent bends is 2mm to 20 mm.

For example, in the detection chip provided in at least one embodiment of the present disclosure, when at least a portion of the curved extension of the first detection flow channel is in a zigzag shape, a bending angle of the bend is 5 ° to 120 °.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the detection unit includes a detection cavity for accommodating the detected sample, and the detection cavity has an air vent and an air-permeable liquid-blocking film covering the air vent.

For example, at least one embodiment of the present disclosure provides a detection chip, wherein the sample injection structure includes a sampling structure mounting portion for receiving a sampling structure.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the sample injection structure further includes a reagent reservoir, the sampling structure can be communicated with the reagent reservoir after the sampling structure is mounted on the sampling structure mounting portion, and the reagent reservoir is further configured to be communicated with the sample filtering structure.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample injection structure further includes a first sealing layer located on a first side of the reagent pool, the first sealing layer is configured to seal the reagent pool on the first side, and after the sampling structure is mounted on the sampling structure mounting portion, the sampling structure may puncture the first sealing layer to communicate with the reagent pool.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample injection structure further includes a second sealing layer located on a second side of the reagent pool, the second sealing layer is used for sealing the reagent pool on the second side, the first side of the reagent pool and the second side of the reagent pool are opposite to each other, and when the second sealing layer is punctured, the reagent pool is communicated with the sample filtering structure.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample injection structure further includes an elastic film located on the second side of the reagent cell, and an action channel interposed between the elastic film and the reagent cell, the action channel allowing an external force to act on the second sealing layer to puncture the second sealing layer when the external force acts on the elastic film to deform the elastic film.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the elastic film is made of a composite polymer material.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the detection chip further includes a first substrate and an adhesive layer, wherein the sampling structure mounting portion and the reagent cell are disposed on the first substrate, and the adhesive layer is attached to a surface of the first substrate and fixes the elastic membrane on the first substrate.

For example, at least one embodiment of the present disclosure provides a detection chip, wherein the adhesive layer at least partially exposes the second sealing layer, so as to allow the deformed elastic film to act on the second sealing layer to puncture the second sealing layer.

For example, at least one embodiment of the present disclosure provides a detection chip further comprising a second substrate, wherein the second substrate is stacked on the first substrate and bonded by the adhesive layer, and the second substrate comprises a substrate opening to provide the action channel.

For example, at least one embodiment of the present disclosure provides that the detection chip further comprises a sample mixing chamber disposed between the sample injection structure and the sample filtration structure.

At least one embodiment of the present disclosure provides a detection system, the detection system is any one of the above detection chip, the sampling structure and the package structure, wherein, the detection chip includes a sampling structure installation part, the sampling structure install in the sampling structure installation part, the package structure is used for sealing the sampling structure.

For example, in the detection system provided in at least one embodiment of the present disclosure, the encapsulation structure is a silicone cap.

For example, in the detection system provided by at least one embodiment of the present disclosure, the package structure includes a sealing portion and a fixing portion, the sealing portion is used for sealing the sampling structure, and the fixing portion is used for fixing the sampling structure on the detection chip.

For example, at least one embodiment of the present disclosure provides a detection system further including a movable first lift pin, where the first lift pin is disposed on a side of the packaging structure away from the sampling structure.

For example, in the detection system provided by at least one embodiment of the present disclosure, the detection chip further includes a reagent reservoir and a second sealing layer, and the detection system further includes a movable second push rod for puncturing the second sealing layer.

Drawings

To more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly introduced below, and it is apparent that the drawings in the following description relate only to some embodiments of the present disclosure and are not limiting to the present disclosure.

Fig. 1 is a perspective view of a detection system provided in accordance with at least one embodiment of the present disclosure;

fig. 2 is a perspective view of an upper substrate of a detection chip according to at least one embodiment of the present disclosure;

fig. 3 is a perspective view of a lower substrate of a detection chip according to at least one embodiment of the present disclosure;

fig. 4 is an exploded top view of a detection system provided in accordance with at least one embodiment of the present disclosure;

fig. 5 is a bottom exploded view of a detection system provided in accordance with at least one embodiment of the present disclosure;

fig. 6 is a schematic plan view of a sample detection structure in a detection chip according to at least one embodiment of the present disclosure;

fig. 7 is a perspective view of a sampling structure in a detection system according to at least one embodiment of the present disclosure;

fig. 8A is a perspective view of a package structure in an inspection system according to at least one embodiment of the present disclosure;

fig. 8B is a bottom view of a package structure in an inspection system according to at least one embodiment of the present disclosure;

FIG. 9 is a schematic diagram of a sample mixing operation performed by a detection system provided in at least one embodiment of the present disclosure;

FIG. 10 is another schematic diagram of a sample mixing operation performed by a detection system according to at least one embodiment of the present disclosure;

fig. 11 is a schematic view of a flow path of a sample to be tested in a testing system according to at least one embodiment of the present disclosure;

fig. 12 is a perspective view of another detection system provided in accordance with at least one embodiment of the present disclosure;

fig. 13 is an exploded top view of a detection system provided in accordance with at least one embodiment of the present disclosure;

fig. 14 is a schematic view of a flow path of a sample to be tested in a testing system according to at least one embodiment of the present disclosure;

fig. 15 is an exploded top view of yet another detection system in accordance with at least one embodiment of the present disclosure.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present disclosure more apparent, the technical solutions of the embodiments of the present disclosure will be described clearly and completely with reference to the drawings of the embodiments of the present disclosure. It is to be understood that the described embodiments are only a few embodiments of the present disclosure, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the disclosure without any inventive step, are within the scope of protection of the disclosure.

Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this disclosure belongs. The use of "first," "second," and similar terms in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.

In the design process of microfluidic chips, it is generally desirable to integrate as many functions of analytical tests on the chip as possible to reduce the dependence of the chip on external operations, thereby achieving automation and integration. For example, the sampling component, the mixing component, the filtering component, and the analysis and detection component of the microfluidic chip can be integrated together to automate the detection process. In the detection process of the microfluidic chip, a sample is firstly obtained by using the sampling part, then the sample and a detection reagent (or a reagent such as a diluent which makes the sample more suitable for detection) are fully mixed in the mixing part, and then filtration is carried out for the next detection operation. The sample filtering operation can improve the purity of the sample, and plays a vital role in the detection process and the detection result of the microfluidic chip.

The detection chip of at least one embodiment of the present disclosure integrates a sample injection function, a sample detection function, and a sample filtration function, thereby enriching the functions of the detection chip, improving the integration level of the detection chip, and contributing to the miniaturization of the detection chip. In addition, the sample filtering structure of the detection chip filters the injected detected sample in a lateral chromatography mode, thereby further contributing to realizing the thinning of the overall appearance of the detection chip. The miniaturized and thinned detection chip contributes to realizing a portable detection and analysis device.

At least one embodiment of the present disclosure provides a detection system, which includes the above detection chip, the sampling structure and the package structure. The detection chip comprises a sampling structure installation part, the sampling structure is installed on the sampling structure installation part, and the packaging structure is used for sealing the sampling structure.

The detection chip and the detection system of the present disclosure are described below with reference to several specific embodiments.

At least one embodiment of the present disclosure provides a detection chip and a detection system, where the detection system includes the detection chip and an assembly used in cooperation with the detection chip. For example, fig. 1 shows a perspective view of a three-dimensional structure of the detection system provided in the embodiment, fig. 2 shows a perspective view of a three-dimensional structure of an upper substrate of the detection chip provided in the embodiment, fig. 3 shows a perspective structure view of a lower substrate of the detection chip provided in the embodiment, fig. 4 shows an exploded top view of the detection system, and fig. 5 shows an exploded bottom view of the detection system provided in the embodiment.

As shown in fig. 1-5, the detection chip 100 includes a sample injection structure 101, a sample detection structure 102, and a sample filtration structure 103. The sample injection structure 101 is used for injecting a sample to be tested. The sample detection structure 102 is used to allow a sample to be detected. For example, the sample detection structure 102 has a detection reagent therein, and the sample to be detected is mixed with the detection reagent and then subjected to a detection operation. For example, in some examples, the detection reagent is a lyophilized reagent, and the sample to be detected can be reconstituted with the lyophilized reagent and subjected to a desired reaction for use in subsequent detection operations, which may be optical detection, etc., as needed, and the embodiments of the present disclosure are not limited thereto. For example, the sample filtering structure 103 is between the sample injection structure 101 and the sample detection structure 102 and is in communication with the sample injection structure 101 and the sample detection structure 102, respectively, to filter the injected sample to be detected in a lateral flow manner and to transmit the filtered sample to be detected to the sample detection structure 102. The filtering mode of the lateral chromatography can fully and uniformly filter the detected sample, so that the filtered detected sample is purer, the detection effect is improved, and meanwhile, the space used by the filtering mode of the lateral chromatography is reduced and is in a thin layer shape, thereby being beneficial to thinning of the detection chip 100.

For example, in some embodiments, the sample filtering structure 103 includes a filter layer 1031, as shown in fig. 3, the filter layer 1031 configured to receive the sample under test from the sample injection structure 101 at a first side 1031A, filter the sample under test in a plane along which the filter layer 1031 lies, and output the filtered sample under test at a second side 1031B opposite the first side 1031A. Thus, the sample to be tested enters the filter layer 1031 from the first side of the filter layer 1031, passes through the filter layer 1031 in the transverse direction, and then is output from the second side of the filter layer 1031, and since the filter path of the sample to be tested is the transverse dimension (length or width) of the filter layer 1031, which is larger or larger than the thickness of the filter layer 1031, the sample to be tested can be sufficiently filtered. For example, when the amount of the sample to be detected is large, the sample to be detected can be sufficiently and uniformly filtered because the filtration paths of the sample to be detected are substantially the same.

For example, in some embodiments, as shown in fig. 1, the sample injection structure 101 and the sample filtration structure 103 are connected by a first channel 104, and the sample filtration structure 103 and the sample detection structure 102 are connected by a second channel 105; alternatively, in other embodiments, the sample injection structure 101 and the sample filtration structure 103 may be directly connected, and the sample filtration structure 103 and the sample detection structure 102 may be directly connected. For example, in some embodiments, the end of the first channel 104 connected to the sample filtering structure 103 includes a first channel cavity 1041, so that the sample to be tested can be uniformly input into the sample filtering structure 103 and the sample to be tested is prevented from being concentrated at the connection of the first channel 104 and the filtering structure 103. For example, the first passage chamber 1041 has a circular shape, an oval shape, a droplet shape (the case shown in the figure), or the like.

For example, in some embodiments, at a first side 1031A of the filter layer, the first channel 104 at least partially overlaps the filter layer 1031 in a direction perpendicular to the plane of the filter layer 1031 to inject the sample to be detected from the sample injection structure 101 into the filter layer 1031 for filtering, whereby the first channel 104 has a larger contact area with the filter layer 1031 to more easily inject the sample to be detected into the filter layer 1031.

For example, as shown in FIGS. 1-3, after the upper substrate and the lower substrate of the detection chip are combined, at least a portion of the first channel 104, such as the first channel chamber 1041, overlaps and interfaces with the filter layer 1031 in a direction perpendicular to the plane of the filter layer 1031. For example, the first passage chamber 1041 extends above the filter layer 1031 to overlap the filter layer 1031. Therefore, the first passage 104 can inject the sample to be detected into the filter layer 1031 along the direction perpendicular to the plane of the filter layer 1031, so that the sample to be detected can be uniformly dispersed before entering the filter layer 1031, thereby avoiding poor filtering effect caused by sample concentration. In addition, the overlapping area of the first passage chamber 1041 and the filter layer 1031 is large, thereby facilitating the dispersion of the detected sample and avoiding the detected sample from concentrating at the connecting position of the filter layer 1031 and the first passage 104. Therefore, the filter structure can fully and uniformly filter the detected sample, and the filtered detected sample is purer.

For example, in some embodiments, on the second side 1031B of the filter layer, the second channel 105 at least partially overlaps the filter layer 1031 in a direction perpendicular to the plane of the filter layer 1031 to receive the filtered sample under test. For example, in some embodiments, at least a portion of the second channel 105 extends above or below the filter layer 1031 to overlap interface with the filter layer 1031. Thus, the second channel 105 can output the sample to be detected from the filter layer 1031 in a direction perpendicular to the plane of the filter layer 1031.

For example, in some embodiments, the first channels 104 and the second channels 105 are connected to different layer surfaces (i.e., lateral surfaces defined by the length and width directions of the filter layer) or the same layer surface, respectively, of the filter layer 1031 in a direction perpendicular to the plane of the filter layer 1031. It should be noted that the layer surfaces referred to in the embodiments of the disclosure refer to surfaces of the filter layer 1031 that are parallel to the plane in which the filter layer 1031 lies, i.e., the upper and lower surfaces of the filter layer 1031 as shown in the figures. For example, the first and

second passages

104, 105 may be attached to the upper and lower surfaces of the filter layer 1031, respectively, or may be attached to both the upper and lower surfaces of the filter layer 1031. For example, in some embodiments, the first channel 104 can also be attached to a surface of a layer of the filter layer 1031, and the second channel 105 can be attached to a side surface of the filter layer 1031, the side surface being a surface between an upper surface and a lower surface of the filter layer 1031. The embodiment of the present disclosure does not limit the specific connection manner of the first channel 104 and the second channel 105, as long as the first channel 104 can inject the sample to be tested to the filter layer 1031 in the direction perpendicular to the plane of the filter layer 1031.

For example, in some embodiments, as shown in fig. 3, sample filtration structure 103 further comprises a filter chamber 1032 that houses a filtration layer 1031. As shown in fig. 1, filter cavity 1032 includes a first opening 1032A and a second opening 1032B, the first opening 1032A being used for inputting a sample to be tested, and the second opening 1032B being used for outputting a filtered sample to be tested. The second opening 1032B is connected to a first end (e.g., the right end as shown) of the second channel 105, and the sample detection structure 102 is connected to a second end (e.g., the left end as shown) of the second channel 105.

For example, in some embodiments, the detection chip further comprises a first substrate and an adhesive layer, the filter chamber 1032 is disposed on the first substrate, the adhesive layer is attached to the surface of the first substrate and fixes the filtration layer 1031 in the filter chamber 1032, and the first substrate can also be used to form/support other functional structures. For example, the detection chip further includes a second substrate, which is stacked on the first substrate and bonded by an adhesive layer, and which may also form/support other functional structures, or the first substrate and the second substrate together form/support other functional structures.

For example, in the embodiment shown in fig. 1 to 5, the detection chip includes an upper substrate 100A and a lower substrate 100B, the lower substrate 100B being implemented as an example of the above-described first substrate, the upper substrate 100A being implemented as an example of the above-described second substrate, and the upper substrate 100A and the lower substrate 100B being bonded by an adhesive layer 100C. For example, the material of the upper substrate 100A may be Polystyrene (PS) or polymethyl methacrylate (PMMA), and the material of the lower substrate 100B may be Polystyrene (PS) or polymethyl methacrylate (PMMA). The adhesive layer 100C may include a material having adhesive properties such as an acrylic adhesive, and may be implemented as a double-sided tape, for example. For example, the upper substrate 100A, the adhesive layer 100C and the lower substrate 100B have substantially the same outer shape, and thus the adhesive layer 100C may better achieve the bonding between the upper substrate 100A and the lower substrate 100B.

For example, the adhesive layer 100C defines a first opening 1032A and a second opening 1032B of the filter chamber 1032, e.g., the adhesive layer 100C has a first opening and a second opening therein, the adhesive layer 100C is attached to the surface of the lower substrate 100B to fix the filter layer 1031 in the filter chamber 1032, and the first opening and the second opening of the adhesive layer 100C are formed as the first opening 1032A and the second opening 1032B of the filter chamber 1032. Thus, the adhesive layer 100C not only serves as an adhesive, but also serves as a seal for the filter cavity 1032, and provides a flow channel for the sample to be tested through the first opening and the second opening, so as to facilitate the lateral chromatography filtering function of the sample filtering structure 103.

For example, the first and

second channels

104 and 105 may be both formed in the upper substrate 100A and communicate with the first and

second openings

1032A and 1032B, respectively. For example, a sample injection structure 101 and a sample detection structure 102 are also disposed on the upper substrate 100A. Thus, in the detection chip shown in fig. 1-5, for example, referring to fig. 11, the sample injection structure 101 located on the upper substrate 100A can input the sample to be detected into the sample filtering structure 103 located on the lower substrate 100B through the first opening 1032A in a direction perpendicular to the plane of the filter membrane 1031, and output the filtered sample to be detected into the sample detection structure 102 located on the upper substrate 100A through the second opening 1032B in a direction perpendicular to the plane of the filter membrane 1031. Thereby realizing the functions of lateral chromatography and vertical liquid feeding of the sample filtering structure 103.

For example, the sample detection structure 102 includes a plurality of detection units 1021 and a plurality of first detection flow channels 1022, and at least one detection unit 1021 is connected to the sample filtration structure 103 through the corresponding first detection flow channel 1022. For example, the detecting unit 1021 is connected to the second end of the second channel 105 (e.g. the left end of the second channel 105 shown in the figure) through the corresponding first detecting flow channel 1022, so that the detected sample filtered by the sample filtering structure 103 can flow into the detecting unit 1021 from the second channel 105 and the first detecting flow channel 1022.

For example, each of the detecting units 1021 is communicated with the second end of the second channel 105 through the corresponding first detecting flow channel 1022. For example, the second end of the second channel 105 has an auxiliary connection structure 1051, and the extension direction of the auxiliary connection structure 1051 is perpendicular to the extension direction of the second channel 105. For example, the first detection flow channels 1022 are all communicated with the second end of the second channel 105 through the auxiliary connection structure 1051, thereby facilitating the arrangement of the first detection flow channels 1022 and making the length, shape, etc. of each first detection flow channel 1022 substantially the same.

For example, in some embodiments, as shown in fig. 6, the width W of the first detection flow channel 1022 can be 0.1mm to 1mm, such as 0.3mm, 0.5mm, or 0.7mm, etc., and the depth (i.e., the dimension in the direction perpendicular to the plane of the upper substrate 100A) of the first detection flow channel 1022 can be 0.1mm to 1mm, such as 0.3mm, 0.5mm, or 0.7mm, etc. Thus, the sample to be tested can have a suitable flow rate and flow rate in the first detection flow channel 1022.

For example, at least a portion of the first detection flow path 1022 may extend in a curved manner. For example, at least a portion of the curved extension of the first detection flow path 1022 has a zigzag shape or an S-shape. For example, at least a portion of the curved extension of the first detection flow channel 1022 may include 2 to 20 bends 1022A, such as 5, 8, 12, 16, etc., and the length L of the flow channel between two adjacent bends 1022A may be 2mm to 20mm, such as 3mm, 5mm, 10mm, or 15mm, etc.

For example, as shown in fig. 6, when at least a portion of the curved extension of the first detection flow path 1022 is a zigzag shape, the bending angle α of the bending 1022A may be 5 ° to 120 °, for example, 30 °, 60 °, or 90 °.

With regard to the design of the first detection flow channel 1022, since the first detection flow channel 1022 has a bent and extended portion, on one hand, the bent and extended portion can extend the flow path of the sample to be detected, and on the other hand, the bend 1022A of the bent and extended portion can increase the flow resistance of the sample to be detected, so that the sample to be detected is not easy to flow back, thereby preventing crosstalk of the sample to be detected among the plurality of detection units 1021, and further preventing detection errors caused by crosstalk of the sample to be detected, and thus the design of the first detection flow channel 1022 can improve the detection accuracy and the detection quality.

For example, in some embodiments, the detection unit 1021 includes a detection chamber (e.g., a cylindrical structure as shown in the figure) for accommodating a sample to be detected, the detection chamber having a vent 1021A and a gas-permeable liquid-blocking film 1021B covering the vent 1021A. When the sample to be detected flows into the detection cavity, the pressure in the detection cavity is increased, the air vent 1021A can exhaust redundant air in the detection cavity to balance air pressure, and the air-permeable liquid-blocking film 1021B has the functions of air permeability and liquid impermeability, so that the sample to be detected can be prevented from flowing out of the detection cavity. When the sensing unit 1021 is formed in the upper substrate 100A, the exhaust hole 1021A may be formed on a side surface of the upper substrate 100A (e.g., a left end surface of the upper substrate 100A as shown in fig. 2 or 4), and the gas-permeable liquid-blocking film 1021B may be attached on the side surface of the upper substrate 100A, thereby covering the exhaust hole 1021A.

For example, in some embodiments, the air permeable liquid blocking films 1021B of the plurality of detection units 1021 covering the air vent 1021A are of an integral structure. At this time, the air-permeable liquid-blocking film 1021B of the integrated structure may cover the whole surface of the side of the plurality of detection units 1021 with the air vent 1021A (as shown in fig. 4 and 5), so that the structure and the manufacturing difficulty of the detection chip may be simplified. For example, the air permeable, liquid resistant membrane 1021B may be an ePTFE (expanded polytetrafluoroethylene) air permeable, liquid resistant membrane, as embodiments of the present disclosure are not limited in this respect.

For example, in some embodiments, the sample injection structure 101 includes a sampling structure mounting portion 1011, the sampling structure mounting portion 1011 for receiving a sampling structure, e.g., mounting a sampling structure, having a sample to be tested therein. For example, the sample injection structure 101 further includes a reagent reservoir 1012, and the reagent reservoir 1012 is used to store a reagent such as a detection reagent or a dilution liquid that makes the sample more suitable for detection (the storage of the dilution liquid in the reagent reservoir 1012 will be described as an example later), and for example, as shown in fig. 4, the reagent reservoir 1012 is formed in the upper substrate 100A. When the sampling structure is mounted to the sampling structure mounting portion 1011, the sampling structure may be in communication with the reagent reservoir 1012, e.g., the sampling structure is further depressed to communicate with the reagent reservoir 1012, and the reagent reservoir 1012 may also communicate with the sample filtration structure 103.

For example, in some embodiments, the ratio of the sample to be tested to the diluent is determined, e.g., the volume of diluent in the reagent reservoir 1012 can be selected and adjusted to facilitate obtaining a desired mixing ratio. For example, the total amount of sample captured by the sampling structure may be known (e.g., the total amount of sample captured by the sampling structure is fixed or can be read), and thus the volume of diluent in the reagent reservoir 1012 may be selected to control the mixing ratio of the sample to be tested and the diluent, to achieve quantification of the sample, to obtain a sample with a certain concentration, etc.

For example, the sample injection structure 101 may further include a first sealing layer 1013 on a first side (shown as the upper side in the figure) of the reagent cell 1012, the first sealing layer 1013 sealing the reagent cell 1012 on the first side. When the sampling structure is mounted to the sampling structure mounting portion 1011, the sampling structure may be pressed to puncture the first sealing layer 1013 to communicate with the reagent reservoir 1012.

For example, the sample injection structure 101 may further comprise a second sealing layer 1014 located at a second side (shown as the lower side in the figure) of the reagent cell 1012, the second sealing layer 1014 being adapted to seal the reagent cell 1012 at the second side, the first side of the reagent cell 1012 and the second side of the reagent cell 1012 facing each other. When the second sealing layer 1014 is ruptured, the reagent reservoir 1012 may be in communication with the sample filtration structure 103.

For example, the first sealing layer 1013 and/or the second sealing layer 1014 may be aluminum foil, and in this case, the first sealing layer 1013 and/or the second sealing layer 1014 may be formed on both sides of the reagent reservoir 1012 by heat sealing, respectively, thereby forming a sealed reagent storage space. For example, in other examples, the first sealing layer 1013 and/or the second sealing layer 1014 may be made of other materials having a sealing function, such as polymethyl methacrylate (PMMA) and polypropylene (PP), and in this case, the first sealing layer 1013 and/or the second sealing layer 1014 may be formed on both sides of the reagent cell 1012 by ultrasonic welding, thereby forming a sealed reagent storage space. Embodiments of the present disclosure do not limit the specific form of the first seal layer 1013 and/or the second seal layer 1014.

For example, in some embodiments, the sample injection structure 101 may further comprise an elastic membrane 1015 and an action channel 1016 located at a second side of the reagent cell 1012, with a second sealing layer 1014 sandwiched between the elastic membrane 1015 and the reagent cell 1012. The adhesive layer 100C is interposed between the elastic film 1015 and the second sealant 1014, thereby adhesively fixing the elastic film 1015 and the second sealant 1014 relative to each other. Meanwhile, referring to fig. 4 and 5, in correspondence with the action passage 1016, the adhesive layer 100C further has a third opening 1001, and the third opening 1001 is also located in a region where the elastic membrane 1015 and the second seal layer 1014 directly face and overlap each other, for example, the third opening 1001 is entirely located in a region where the elastic membrane 1015 and the second seal layer 1014 directly face and overlap each other, and the region where the elastic membrane 1015 and the second seal layer 1014 directly face and overlap each other is entirely fixed by the adhesive layer 100C. The action channels 1016 allow the deformed elastic membrane 1015 to partially pass through the third opening 1001 of the adhesive layer 100C when an external force acts on the elastic membrane 1015 to deform the elastic membrane 1015, thereby enabling the external force to also act on the second sealant layer 1014 to puncture the second sealant layer 1014 without the elastic membrane 1015 itself being punctured, and the elastic membrane 1015 will substantially recover its original shape when the external force is removed.

For example, the material of the elastic film 1015 is a composite polymer material, such as a composite material of Polystyrene (PS) and polyethylene terephthalate (PET). Therefore, the elastic membrane 1015 can have better elasticity and strength at the same time. The elastic film 1015 is bonded between the upper substrate and the lower substrate, for example, by an adhesive layer 100C. For example, in other examples, the elastic film 1015 may also be bonded between the upper substrate and the lower substrate by ultrasonic welding, photo adhesive bonding, chemical solvent bonding, laser welding, or the like, which is not limited in this disclosure.

For example, as described above, the adhesive layer 100C at least partially exposes the second seal layer 1014 through the third opening 1001 to allow the deformed elastic membrane 1015 to act on the second seal layer 1014 to puncture the second seal layer 1014. For example, the lower substrate 100B comprises a substrate opening to provide the active channel 1016, e.g. the elastic membrane 1015 completely covers the active channel 1016, and a projection of the third opening 1001 of the adhesive layer 100C on the lower substrate 100B, e.g. at least partially overlaps the active channel 1016, e.g. completely covers the active channel 1016.

For example, as shown in fig. 1 to fig. 5, the detection system provided in this embodiment further includes a sampling structure 110 and a package structure 111 in addition to the detection chip. The sampling structure 110 is mounted to the sampling structure mounting portion 1011 of the sample injection structure 101, and the packaging structure 111 is used to seal the sampling structure 110. For example, as shown in fig. 4 and 5, the sampling structure 110 can be mounted to the sampling structure mounting portion 1011 via a gasket 140, such as a silicone material, which can provide a sealing and cushioning effect.

For example, in some embodiments, as shown in FIG. 7, the sampling structure 110 can be a sampling needle having sample aspirating and mixing functions. For example, as shown in fig. 8A and 8B, the encapsulation structure 111 may be a silicone cap. For example, the sampling structure 110 and the encapsulation structure 111 may cooperate to perform a sample mixing function.

For example, fig. 9 shows a schematic cross-sectional view of the sample injection structure 101 when the sampling structure 110 is mounted to the sampling structure mounting portion 1011 and the packaging structure 111 seals the sampling structure 110. As shown in fig. 9, the sampling structure 110 includes a first aspiration channel 110A, a second aspiration channel 110B, and a chamber 110C. A plurality of partition pillars 110D are provided in the second suction passage 110B. The partition column 110D has a first gap from the channel wall of the second suction channel 110B, and the adjacent partition column 110D has a second gap. The first and

second aspiration channels

110A, 110B of the sampling structure are configured to draw a sample to be tested (e.g., blood, body fluid, etc.) by capillary action, and the second aspiration channel 110B has a separation column 110D therein to split the sample to be tested flowing through the second aspiration channel 110B. In the chamber 110C, the sampling structure can perform a sample mixing function after the sample to be detected is mixed with the diluent in the reagent pool.

For example, as shown in fig. 8A and 8B, the package structure 111 includes a main body portion 111A and at least one exhaust hole 111B, such as a plurality of exhaust holes 111B, disposed at a peripheral position of the main body portion 111A. For example, the exhaust holes 111B are evenly distributed around the body portion 111A. The exhaust hole 110B is configured to be opened or closed when the main body 111A receives different forces. For example, in some embodiments, as shown in fig. 8B, the exhaust hole 110B may be a triangular prism exhaust hole, and when the main body portion 111A receives a force greater than or equal to a threshold value, the exhaust hole 110B is closed, and when the main body portion 2011 receives a force less than the threshold value, the exhaust hole 110B is in an open state. The internal pressure of the sampling structure 110 can thus be adjusted by applying different forces to the body portion 111A of the encapsulation structure 111.

For example, in some embodiments, the detection system further comprises a movable first push rod 120, the first push rod 120 being disposed on a side of the encapsulation structure 111 away from the sampling structure 110. Thereby, the first lift pin 120 can move up and down on the body portion 111A of the package structure 111 to apply a biasing force to the body portion 111A. For example, the force exerted by the first push rod 120 on the body portion 111A can be adjusted, thereby controlling the pressure inside the sampling structure 110. For example, the movement of the first lift pin 120 may be driven by a driving means (e.g., a stepping motor).

For example, as shown in fig. 8A, the body portion 111A may include a groove for guiding the position of the urging force of the first jack 120. For example, the recess groove is the same shape as the first ram 120, e.g., circular in cross-section. For example, the groove has a diameter slightly larger than that of the first lift pin 120 and has a step, thereby facilitating guiding of the force application position of the first lift pin 120.

For example, in some embodiments, the detection system can further include a movable second push-bar 130, the second push-bar 130 for rupturing the second sealing layer 1014. For example, the second lift pin 130 applies a force to the elastic membrane 1015 through the action channel 1016 so that the deformed elastic membrane 1015 can act on the second seal layer 1014 to puncture the second seal layer 1014. For example, the movement of the second lift pin 130 may be driven by another driving means (e.g., a stepping motor).

The operation of the above-described detection system will now be described with reference to fig. 9-11.

First, the sample to be tested is sucked by the sampling structure 110, and at this time, the sample to be tested can be sucked from the first suction channel 110A and the second suction channel 110B of the sampling structure 110 by capillary action. The sample to be tested may be, for example, blood, body fluid, etc., and embodiments of the present disclosure are not limited thereto.

Then, the sampling structure 110 is mounted on the detection chip 100, and the packaging structure 111 is used to fix and seal the sampling structure 110. For example, after the sampling structure 110 is mounted on the detection chip 100, the bottom of the sampling structure 110 extends into the reagent reservoir 1012 of the detection chip 100. For example, the reagent cell 1012 has a first sealing layer 1013 for sealing on the upper surface, and when the sampling structure 110 is mounted on the detection chip 100, the sampling structure 110 can puncture the first sealing layer 1014 to communicate with the reagent cell 1012, so that the sample to be detected can be mixed with the diluent in the reagent cell 1012. After the sampling structure 110 and the sealing structure 111 are mounted, two communicated sealed cavities are formed in the detection chip 100, one is a first sealed cavity formed by the sampling structure 110, and the other is a second sealed cavity formed by the reagent cell 1012.

As shown in fig. 9, when the first push rod 120 is used to apply a force to the main body 111A of the sealing structure 111 at a first speed, and the force is weak and is less than the threshold pressure, the vent hole 111B of the sealing structure 111 is in an open state, and the sampling structure 110 can discharge air or spilled liquid through the vent hole 111B. When the applied force is increased gradually to be equal to or greater than the threshold pressure, the vent hole 111B is in a closed state, and at this time, the pressure inside the sampling structure 110 is increased, so that the sample to be detected is pushed out of the sampling structure 110 and into the reagent reservoir 1012, as indicated by the arrow in fig. 9. Thus, the sample to be tested can be mixed with the diluent in the reagent reservoir 1012.

As shown in fig. 10, when the first plunger 120 is withdrawn at the second speed, the main body 111A rebounds, and the mixture of the diluent and the sample to be detected in the reagent tank 1012 is sucked back into the sampling structure 110. Since the second suction channel 110B of the sampling structure 110 has the separation pillars 110D, and a plurality of narrow gaps are formed between adjacent separation pillars 110D and between the separation pillars 110D and the channel wall, the flow rate of the mixture of the diluent and the sample to be detected is increased after passing through the first suction channel 110A and the second suction channel 110B, and the mixture can be flushed into the chamber 110C of the sampling structure 110 at a higher speed and forms a swirling mixture with the sample to be detected in the sampling structure 110, as shown by the arrows in fig. 10, thereby improving the mixing efficiency and making the mixture of the diluent and the sample to be detected more uniform. At this point, one mixing operation is completed.

For example, the first speed at which the first push rod 120 applies force to the main body portion 111A of the sealing structure 111 is greater than the second speed at which the first push rod 120 retracts, that is, the operation of rapidly pressing down and slowly lifting up the first push rod 120 is adopted, which helps to improve the mixing effect of the sample to be detected and the diluent in the sampling structure 110.

For example, the mixing operation may be performed multiple times to further improve the mixing effect of the sample to be tested and the diluent.

For example, when the sample to be tested and the diluent are sufficiently mixed, the second plunger 130 may be driven to move upward, so that the second plunger 130 applies a force to the second sealing layer 1014 below the reagent cell 1012 through the action channel 1016 and the elastic membrane 1015 to puncture the second sealing layer 1014. Since the elastic film 1015 has elasticity, it can be restored to its original state after the external force is removed. After the second sealing layer 1014 is ruptured, the reagent reservoir 1012 is in communication with the sample filtration structure 103.

For example, fig. 11 shows the flow path of the sample being tested in more detail. As shown in fig. 11, the first push rod 120 can be continuously pushed downwards slowly to make the sample to be tested enter the sample filtering structure 103 (the sample to be tested is inputted into the filtering membrane 1031, for example, in a direction perpendicular to the plane of the filtering membrane 1031), and the filtered sample to be tested can be transferred to the sample detecting structure 102 (the sample to be tested is outputted into the sample detecting structure 102, for example, in a direction perpendicular to the plane of the filtering membrane 1031) during the process that the first push rod 120 is continuously pushed downwards, for example, to the detection chamber 1021 of the sample detecting structure 102. For example, the detection chamber 1021 has lyophilized reagents suitable for different detection items, so that after the sample to be detected can react with the lyophilized reagents, the sample detection structure 102 starts to perform detection and outputs a detection result. For example, during the testing process of the sample testing structure 102, the first plunger 120 is always kept in the pressing state to avoid the backflow of the tested sample.

Therefore, the detection system can realize an automatic detection process, and can obtain a more accurate detection result.

For example, in some embodiments, the detection chip 100 may further include a sample mixing chamber (not shown) disposed between the sample injection structure 101 and the sample filtration structure 103. At this time, the sample to be tested and the diluent may also be mixed in the sample mixing chamber. For example, the mixing operation may be performed by both the sampling structure 110 and the sample mixing chamber, or in some embodiments, the sampling structure 110 may have only a sampling function, so that the mixing operation may be performed only in the sample mixing chamber, which is not limited by the embodiments of the present disclosure.

At least one embodiment of the present disclosure provides another detection chip and detection system, where the detection system includes the detection chip. For example, fig. 12 shows a perspective view of a detection system provided by the embodiment, and fig. 13 shows an exploded top view of the detection system.

As shown in fig. 12 and 13, the detection chip 200 includes a sample injection structure 201, a sample detection structure 202, and a sample filtration structure 203. The sample injection structure 201 is used for injecting a sample to be detected, the sample detection structure 202 is used for enabling the sample to be detected, and the sample filtering structure 203 is arranged between the sample injection structure 201 and the sample detection structure 20 and is respectively communicated with the sample injection structure 201 and the sample detection structure 202 so as to filter the injected sample to be detected in a lateral chromatography mode and transmit the filtered sample to be detected to the sample detection structure 202.

For example, in some embodiments, the sample filtering structure 203 includes a filter layer 2031, the filter layer 2031 configured to receive the sample under test from the sample injection structure 201 on a first side 2031A, filter the sample under test in a plane along which the filter layer 2031 lies, and output the filtered sample under test on a second side 2031B opposite the first side 2031A, whereby the filter layer 2031 may perform a lateral flow filtration function.

For example, the sample injection structure 201 and the sample filtration structure 203 are connected by a first channel 204, and the sample filtration structure 203 and the sample detection structure 202 are connected by a second channel 205.

For example, on a first side 2031A of the filter layer 2031, the first channel 204 at least partially overlaps the filter layer 2031 in a direction perpendicular to the plane of the filter layer 2031 to inject the sample to be tested from the sample injection structure into the filter layer 2031 for filtration. For example, first channel 204 overlaps filter layer 2031 on both the side and top of filter layer 2031 so that the sample under test can enter filter layer 2031 both laterally and in a direction perpendicular to the plane of filter layer 2031. Therefore, the sample to be detected can be uniformly dispersed before entering the filtering layer 2031, so as to avoid poor filtering effect caused by sample concentration.

For example, the end of the first channel connected to the sample filtration structure 203 may also have a first channel cavity 2041, the first channel cavity 2041 for example overlapping the filter layer 2031 on the top and side of the filter layer 2031. The first passage cavity 2041 is, for example, circular, oval, or droplet-shaped (the case shown in fig. 12), or the like. Since the overlapping area of the first channel cavity 2041 and the filter layer 2031 is large, the dispersion of the detected sample is facilitated, and the detected sample is prevented from being concentrated at the connecting position of the filter layer 2031 and the first channel 204. Therefore, the filter structure can fully and uniformly filter the detected sample, and the detected sample is purer.

For example, the sample filtration structure 203 further includes a filter cavity 2032 that houses a filter layer 2031, the filter cavity 2032 including a first opening 2032A and a second opening 2032B. The first aperture 2032A is used for inputting the sample to be tested, and the second aperture 2032B is used for outputting the filtered sample to be tested. For example, the second opening 2032B is connected to a first end of the second channel 205 and the sample detection structure 202 is connected to a second end of the second channel 205.

For example, the detecting chip further includes a first substrate and an adhesive layer, the filter cavity 2032 is disposed on the first substrate, and the adhesive layer is attached to the surface of the first substrate and fixes the filter layer 2031 in the filter cavity 2032. For example, the detection chip further includes a second substrate, which is stacked on the first substrate and bonded thereto by an adhesive layer.

For example, as shown in fig. 13, the detection chip includes an upper substrate 200A and a lower substrate 200B, the upper substrate 200A being implemented as an example of the above-described first substrate, the lower substrate 200B being implemented as an example of the above-described second substrate, and the upper substrate 200A and the lower substrate 200B being bonded by an adhesive layer 200C. For example, the upper substrate 200A, the adhesive layer 200C, and the lower substrate 200B have substantially the same outer shape, and thus the adhesive layer 200C may better achieve the bonding between the upper substrate 200A and the lower substrate 200B. The materials of the upper substrate 200A, the lower substrate 200B, and the adhesive layer 200C can be referred to the above embodiments, and are not described herein again.

For example, the first and

second passages

204 and 205 are provided in the upper substrate 200A. For example, a sample injection structure 201 and a sample detection structure 202 are also provided in the upper substrate 200A. At this time, the sample injection structure 101 can input the detected sample into the sample filtering structure 203 from the side direction and the direction perpendicular to the plane of the filtering membrane 2031 through the first opening 2032A, and output the filtered detected sample into the sample detecting structure 102 from the side direction through the second opening 1032B. Thereby realizing the functions of lateral chromatography and vertical liquid feeding of the sample filtering structure 103.

For example, the sample detection structure 202 includes a plurality of detection units 2021 and a plurality of first detection flow channels 2022, and at least one detection unit 2021 is connected to the sample filter structure 203 through the corresponding first detection flow channel 2022. The structure, the size parameter, the connection mode, and the like of the first detection flow channel 2022 can be referred to the above embodiments, and are not described herein again.

For example, the detection unit 2021 includes a detection chamber for accommodating a sample to be detected, the detection chamber having an exhaust hole 2021A and a gas-permeable liquid-blocking film 2021B covering the exhaust hole 2021A. The gas-permeable liquid-blocking film 2021B has a gas-permeable but liquid-impermeable function, and thus can prevent the sample to be tested from flowing out of the detection chamber.

For example, in some embodiments, the air-permeable liquid-blocking films 2021B of the plurality of detection units 2021 covering the exhaust holes 2021A are of an integral structure, and at this time, the air-permeable liquid-blocking films 2021B of the integral structure can cover the whole surface of the side (as shown in fig. 13) of the plurality of detection units 2021 having the exhaust holes 2021A, so that the structure and the manufacturing difficulty of the detection chip can be simplified.

For example, in some embodiments, the sample injection structure 201 includes a sampling structure mounting portion 2011 for receiving, e.g., mounting, a sampling structure. For example, the sample injection structure 201 further includes a reagent reservoir 2012, and when the sampling structure is mounted to the sampling structure mounting portion 2011, the sampling structure can be in communication with the reagent reservoir 2012, and the reagent reservoir 2012 can also be in communication with the sample filtration structure 203.

For example, in some embodiments, the sample injection structure 201 can further include a first sealing layer 2013 on a first side (shown as the top side in the figure) of the reagent well 2012, the first sealing layer 2013 for sealing the reagent well 2012 on the first side. After the sampling structure is mounted to the sampling structure mount 2011, the sampling structure can puncture the first sealing layer 2013 to communicate with the reagent reservoir 2012.

For example, the sample injection structure 201 may further include a second sealing layer 2014 located at a second side (shown as a lower side in the figure) of the reagent well 2012, the second sealing layer 2014 being used for sealing the reagent well 2012 at the second side, and the first side of the reagent well 2012 and the second side of the reagent well 2012 are directly opposite to each other. When the second sealing layer 2014 is ruptured, the reagent reservoir 2012 communicates with the sample filtration structure 203.

For example, in some embodiments, the sample injection structure 201 may further include an elastomeric film 2015 and an active channel 2016 located on a second side of the reagent reservoir 2012, with a second sealing layer 2014 sandwiched between the elastomeric film 2015 and the reagent reservoir 2012. The adhesive layer 100C is interposed between the elastic film 2015 and the second seal layer 2014, thereby adhesively fixing the elastic film 2015 and the second seal layer 2014 relative to each other. Meanwhile, referring to fig. 13, in correspondence with the active passage 2016, the adhesive layer 200C also has an opening 2001, the opening 2001 also being located in a region where the elastic film 2015 and the second sealing layer 2014 are in direct opposition and overlap with each other, for example, the opening 2001 is entirely located in a region where the elastic film 2015 and the second sealing layer 2014 are in direct opposition and overlap with each other, and the region where the elastic film 2015 and the second sealing layer 2014 are in direct opposition and overlap with each other is entirely fixed by the adhesive layer 200C. The active channel 2016 allows the deformed elastic film 2015 to partially pass through the opening 2001 of the adhesive layer 200C when an external force acts on the elastic film 2015 to deform the elastic film 2015, thereby enabling the external force to also act on the second seal layer 2014 to puncture the second seal layer 2014 without the elastic film 2015 itself being punctured, and the elastic film 2015 will substantially recover to its original shape when the external force is removed.

For example, the material of the elastic film 2015 may be a composite polymer material, such as a composite material of Polystyrene (PS) and polyethylene terephthalate (PET). Therefore, the elastic film 2015 can have better elasticity and strength at the same time.

For example, a sampling structure mounting portion 2011 and a reagent reservoir 2012 are provided on the upper substrate 200A, and an adhesive layer 200C is attached on a surface (shown as a lower surface in the drawing) of the upper substrate 200A and fixes an elastic film 2015 on the surface of the upper substrate 200A. For example, the lower substrate 200B includes a substrate opening to provide an active channel 2016, e.g., an elastomeric film 2015 completely covering the active channel 2016.

For example, adhesive layer 200C at least partially exposes second seal layer 2014 through opening 2001 to allow a deformable elastomeric film 2015 to be able to act on second seal layer 2014 to puncture second seal layer 2014. The projection of the third opening 1001 of the adhesive layer 200C onto the lower substrate 100B, for example, at least partially overlaps the active channel 1016, e.g., is located entirely within the active channel 1016.

For example, in some embodiments, as shown in fig. 12, the detection chip 200 may further comprise a sample mixing chamber 206 disposed between the sample injection structure 201 and the sample filtration structure 203. The sample mixing chamber 206 may be used to mix the sample being tested with the diluent in the reagent reservoir.

For example, the detection system provided in this embodiment further includes a sampling structure 210 and a package structure 211 in addition to the detection chip 200. The sampling structure 210 is mounted to the sampling structure mounting 2011 and the packaging structure 211 is configured to seal the sampling structure 210. For example, the encapsulation structure 211 is a silicone cap, so that the encapsulation structure 211 has better elasticity and sealing performance, and the sample can be mixed by matching the sampling structure 210 and the encapsulation structure 211.

For example, in some embodiments, as shown in fig. 12, the package structure 211 includes a sealing portion 211A and a fixing portion 211B, the sealing portion 211A is used for sealing the sampling structure 210, and the fixing portion 211B is used for fixing the sampling structure 210 on the detection chip 200. For example, the sealing portion 211A and the fixing portion 211B are of an integrally formed silicone structure. The fixing structure 211B is a ring-shaped socket structure.

For example, the sealing portion 211A may also have a main body portion and an exhaust hole, which may be referred to the package structure 111 provided in the above embodiments, and details are not described herein.

For example, in some embodiments, the detection system further comprises a movable first push rod 220, the first push rod 220 being disposed on a side of the encapsulation structure 211 remote from the sampling structure 210. The first lift pins 220 may apply a force to the sealing portion 211A of the encapsulation structure 211.

For example, the detection system can further include a second removable mandrel 230, the second mandrel 230 being used to puncture the second sealing layer 2014. For example, the second lift pins 230 may apply a force to the second sealing layer 2014 through the action passage 2016 and the elastic mold 2015 in the lower substrate 100B.

The operation of the above-described detection system will now be described with reference to fig. 14.

First, the sample to be tested is aspirated using the sampling structure 210. For example, the sampling structure 210 may be any structure having a sampling function. The sample to be tested may be, for example, blood, body fluid, etc., and embodiments of the present disclosure are not limited thereto.

Then, the sampling structure 210 is mounted on the detection chip 200, and the packaging structure 211 is adopted to fix and seal the sampling structure 210. For example, after the sampling structure 210 is mounted on the detection chip 200, the bottom of the sampling structure 210 extends into the reagent well 2012 of the detection chip 200. For example, the reagent well 2012 has a first sealing layer 2013 for sealing on the upper surface thereof, and when the sampling structure 210 is mounted on the detection chip 200, the sampling structure 210 can puncture the first sealing layer 2014, so as to communicate with the reagent well 2012, so that the sample to be tested can be mixed with the diluent in the reagent well 2012.

The second plunger 230 is driven to move upward so that the second plunger 230 applies a force to the second sealing layer 2014 under the reagent reservoir 2012 through the action channel 2016 and the elastic film 2015 to puncture the second sealing layer 2014. Since the elastic film 2015 has elasticity, it can be restored to its original state after the external force is removed. After the second sealing layer 2014 is ruptured, the reagent cell 1012 is in communication with the sample mixing chamber 206.

When a force is applied to the sealing portion 211A of the sealing structure 211 at a first speed by using the first push rod 220, the pressure inside the sampling structure 210 increases, so that the sample to be tested is pushed out from the sampling structure 210 and enters the reagent pool 2012, and further, the sample to be tested and the diluent in the reagent pool 2012 can be mixed and can enter the sample mixing chamber 206.

Thereafter, the first plunger 220 is retracted at the second speed, and at this time, the sealing portion 211A is rebounded, and the mixture of the diluent and the sample to be detected is sucked back into the reagent well 2012. Thus, the mixture of the diluent and the sample to be detected can reciprocate in the reagent tank 2012 and the mixing chamber 206, thereby completing the mixing operation.

For example, the first speed of the first push rod 220 applying force to the sealing portion 211A is less than the second speed of the first push rod 220 withdrawing, that is, the operation of slowly pressing down and rapidly lifting up the first push rod 220 is adopted, and the operation helps to improve the mixing effect of the sample to be detected and the diluent.

For example, the mixing operation may be performed multiple times, so that the mixed solution performs multiple reciprocating motions in the reagent tank 2012 and the mixing chamber 206, so as to further improve the mixing effect of the sample to be detected and the diluent.

For example, fig. 14 shows the flow path of the sample to be tested in the test chip 200 of the above embodiment. As shown in fig. 13, the sample to be tested can be continuously introduced into the sample filtering structure 203 by the slow and continuous pressing operation of the first push rod 220 (the sample to be tested is introduced into the filtering membrane 2031 from the lateral direction and the direction perpendicular to the plane of the filtering membrane 2031, for example), and the filtered sample to be tested can be conveyed to the sample testing structure 202 (the sample to be tested is discharged to the sample testing structure 202 from the lateral direction, for example) and finally conveyed into the testing cavity 2021 of the sample testing structure 202 while the first push rod 220 is continuously pressed. For example, the different detection chambers 2021 have lyophilized reagents suitable for different detection items, so that after the sample to be detected can react with the lyophilized reagents, the sample detection structure 202 starts to perform detection and outputs the detection result. For example, during the testing process of the sample testing structure 202, the first plunger 220 is always kept in the pressing state to avoid the backflow of the tested sample.

For example, in some embodiments, the detection system may also be used with sampling structures having both acquisition and mixing functions, such as the sampling structures shown in fig. 7, 9-10, and in some examples, the sealing structures shown in fig. 8A and 8B. At this time, the detection system may also perform the detection operation by an operation procedure similar to that provided in the above-described embodiment. The operation process of the detection system is not particularly limited by the embodiments of the present disclosure.

At least one embodiment of the present disclosure provides a detection chip and a detection system, wherein the detection system includes the detection chip. For example, fig. 15 shows a top-view exploded view of the detection system provided by this embodiment. The detection chip of the detection system of this embodiment is a detection chip and detection system modification shown in fig. 12 and 13, and differs from the detection chip and detection system shown in fig. 12 and 13 in that the detection chip includes only the upper substrate 300A without including the lower substrate, while the elastic film 3015 located on the second side (lower side in the figure) of the reagent reservoir covers the lower surface of the upper substrate 300A in the figure, rather than only the reagent reservoir and its peripheral region, whereby the elastic film 3015 also functions as the lower substrate in the detection chip shown in fig. 12 and 13. The same parts of the embodiment shown in fig. 15 as those of the embodiments shown in fig. 12 and 13 will not be described again here.

For example, the material of the elastic film 3015 may be a composite polymer material, such as a composite material of Polystyrene (PS) and polyethylene terephthalate (PET). Therefore, the elastic film 2015 can have better elasticity and strength at the same time.

An adhesive layer 300C is attached on the lower side surface of the upper substrate 300A in the drawing and fixes the elastic film 3015 on the lower side surface of the upper substrate 300A, the adhesive layer 300C further having an opening 3001. The lift pins may directly act on the elastic die 3015 to apply a force to the sealing layer on the reagent reservoir side through the openings 3001 of the adhesive layer 300C.

The following points need to be explained:

(1) the drawings of the embodiments of the disclosure only relate to the structures related to the embodiments of the disclosure, and other structures can refer to the common design.

(2) For purposes of clarity, the thickness of layers or regions in the figures used to describe embodiments of the present disclosure are exaggerated or reduced, i.e., the figures are not drawn on a true scale.

(3) Without conflict, embodiments of the present disclosure and features of the embodiments may be combined with each other to arrive at new embodiments.

The above description is only for the specific embodiments of the present disclosure, but the scope of the present disclosure is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present disclosure, and all the changes or substitutions should be covered within the scope of the present disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.

Claims

1. A detection chip, comprising:

the sample injection structure is used for injecting a sample to be detected;

a sample detection structure for allowing the sample to be detected,

and the sample filtering structure is arranged between the sample injection structure and the sample detection structure and is respectively communicated with the sample injection structure and the sample detection structure so as to filter the injected detected sample in a lateral chromatography manner and transmit the filtered detected sample to the sample detection structure.

2. The detection chip of claim 1, wherein the sample filtration structure comprises a filtration layer,

the filter layer is configured to receive the test sample from the sample injection structure at a first side, filter the test sample in a plane along which the filter layer lies, and output the filtered test sample at a second side opposite the first side.

3. The detection chip of claim 2, wherein the sample injection structure and the sample filtration structure are connected by a first channel,

the sample filtration structure and the sample detection structure are connected by a second channel.

4. The detection chip according to claim 3, wherein the first channel at least partially overlaps and interfaces with the filter layer in a direction perpendicular to a plane of the filter layer at the first side of the filter layer, so as to inject the detected sample from the sample injection structure into the filter layer for filtering.

5. The detection chip according to claim 4, wherein the second channel at the second side of the filter layer at least partially overlaps and interfaces with the filter layer in a direction perpendicular to a plane of the filter layer to receive the filtered sample to be detected.

6. The detection chip according to claim 5, wherein the first channel and the second channel are connected to different layer surfaces or the same layer surface of the filter layer in a direction perpendicular to the plane of the filter layer.

7. The detection chip according to claim 3, wherein the sample filtration structure further comprises a filter chamber for accommodating the filter layer, the filter chamber comprising a first opening for inputting the sample to be detected and a second opening for outputting the filtered sample to be detected,

wherein the second opening is connected to a first end of the second channel and the sample detection structure is connected to a second end of the second channel.

8. The detection chip of claim 7, further comprising a first substrate and an adhesive layer, wherein the filter cavity is disposed on the first substrate, and the adhesive layer is attached to a surface of the first substrate and fixes the filter layer in the filter cavity.

9. The detection chip of claim 8, wherein the first channel and the second channel are disposed in the first substrate.

10. The detection chip of claim 9, wherein the sample injection structure and the sample detection structure are disposed on the first substrate.

11. The detection chip according to claim 8, further comprising a second substrate, wherein the second substrate is laminated with the first substrate and bonded by the adhesive layer,

the adhesive layer defines the first opening and the second opening of the filter cavity,

the first channel and the second channel are formed in the second substrate and communicate with the first opening and the second opening, respectively.

12. The detection chip of claim 11, wherein the sample injection structure and the sample detection structure are disposed on the second substrate.

13. The detecting chip of any one of claims 1 to 12, wherein the sample detecting structure comprises a plurality of detecting units and a plurality of first detecting flow channels,

at least one detection unit is communicated with the sample filtering structure through a corresponding first detection flow channel.

14. The detecting chip of claim 13, wherein the width of the first detecting flow channel is 0.1mm-1mm, and the depth is 0.1mm-1 mm.

15. The detection chip of claim 13, wherein at least a portion of the first detection flow channel extends in a curved manner.

16. The detection chip of claim 15, wherein at least a portion of the curved extension of the first detection flow channel is in a zigzag shape or an S-shape.

17. The detection chip of claim 16, wherein at least a portion of the curved extension of the first detection flow channel comprises 2-20 bends, and the length of the flow channel between two adjacent bends is 2mm-20 mm.

18. The detecting chip according to claim 17, wherein when at least a portion of the curved extension of the first detecting flow channel is in a zigzag shape, a bending angle of the bend is 5 ° to 120 °.

19. The detection chip according to claim 13, wherein the detection unit comprises a detection chamber for accommodating the sample to be detected,

the detection cavity is provided with an exhaust hole and a permeable liquid-blocking film covering the exhaust hole.

20. The detection chip of any one of claims 1-7, wherein the sample injection structure comprises a sampling structure mount for receiving a sampling structure.

21. The detection chip of claim 20, wherein the sample injection structure further comprises a reagent reservoir,

when the sampling structure is arranged on the sampling structure mounting part, the sampling structure can be communicated with the reagent pool, and

the reagent reservoir is also configured to be communicable with the sample filtration structure.

22. The detection chip of claim 21, wherein the sample injection structure further comprises a first sealing layer located on a first side of the reagent reservoir,

the first sealing layer is for sealing the reagent reservoir at the first side,

when the sampling structure install in behind the sampling structure installation department, the sampling structure can puncture first sealing layer in order with reagent pond intercommunication.

23. The detection chip of claim 22, wherein the sample injection structure further comprises a second sealing layer located on a second side of the reagent reservoir,

the second sealing layer is used for sealing the reagent pool on the second side, and the first side of the reagent pool and the second side of the reagent pool are opposite to each other,

the reagent reservoir is in communication with the sample filtration structure after the second sealing layer is ruptured.

24. The detection chip according to claim 23, wherein the sample injection structure further comprises an elastic membrane and an action channel on a second side of the reagent reservoir, the second sealing layer being interposed between the elastic membrane and the reagent reservoir,

the action passage allows an external force to act also on the second sealing layer to puncture the second sealing layer when the external force acts on the elastic film to deform the elastic film.

25. The detecting chip according to claim 24, wherein the material of the elastic membrane is a composite polymer material.

26. The detecting chip according to claim 24, further comprising a first substrate and an adhesive layer, wherein the sampling structure mounting portion and the reagent cell are disposed on the first substrate, and the adhesive layer is attached to a surface of the first substrate and fixes the elastic membrane on the first substrate.

27. The detection chip of claim 26, wherein the adhesive layer at least partially exposes the second sealing layer to allow the deformed elastic membrane to act on the second sealing layer to puncture the second sealing layer.

28. The detection chip according to claim 26, further comprising a second substrate, wherein the second substrate is laminated with the first substrate and bonded by the adhesive layer,

the second substrate includes a substrate opening to provide the active channel.

29. The detection chip of any one of claims 1-12, further comprising a sample mixing chamber disposed between the sample injection structure and the sample filtration structure.

30. An inspection system comprising the inspection chip, the sampling structure and the packaging structure of any one of claims 1 to 19 and 29,

wherein, the detection chip includes the sampling structure installation department, the sampling structure install in the sampling structure installation department, packaging structure is used for sealing the sampling structure.

31. The detection system of claim 30, wherein the encapsulation structure is a silicone cap.

32. The inspection system of claim 31, wherein the encapsulation structure includes a sealing portion and a securing portion,

the sealing part is used for sealing the sampling structure, and the fixing part is used for fixing the sampling structure on the detection chip.

33. The detection system of claim 32, further comprising a movable first push rod disposed on a side of the encapsulation structure distal from the sampling structure.

34. The detection system of claim 33, wherein the detection chip further comprises a reagent reservoir and a second sealing layer,

the detection system further comprises a movable second push rod for puncturing the second sealing layer.

35. An inspection system comprising the inspection chip, the sampling structure and the packaging structure of any one of claims 20 to 28,

wherein, sampling structure install in detect the sampling structure installation department of chip, packaging structure is used for sealing sampling structure.