CN113318261A - Preparation method and application of chitosan derivative fiber membrane loaded with capsaicin - Google Patents
Preparation method and application of chitosan derivative fiber membrane loaded with capsaicin Download PDFInfo
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- CN113318261A CN113318261A CN202110588817.5A CN202110588817A CN113318261A CN 113318261 A CN113318261 A CN 113318261A CN 202110588817 A CN202110588817 A CN 202110588817A CN 113318261 A CN113318261 A CN 113318261A
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0015—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/08—Polysaccharides
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F9/00—Artificial filaments or the like of other substances; Manufacture thereof; Apparatus specially adapted for the manufacture of carbon filaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/204—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with nitrogen-containing functional groups, e.g. aminoxides, nitriles, guanidines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/402—Anaestetics, analgesics, e.g. lidocaine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/41—Anti-inflammatory agents, e.g. NSAIDs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
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Abstract
The invention discloses a preparation method and application of a chitosan derivative fiber membrane loaded with capsaicin, which comprises the following steps: (1) preparing catechol modified chitosan derivative by using 3, 4-dihydroxyphenyl propionic acid and 1-ethyl-3- (3-dimethyl aminopropyl) carbodiimide hydrochloride modified chitosan, and storing after freeze drying; (2) preparing capsaicin-loaded catechol chitosan by using the catechol-modified chitosan derivative prepared in the step (1), so that capsaicin is loaded in the chitosan derivative; (3) and (3) carrying out a spinning process on the chitosan derivative loaded with the capsaicin in the step (2) by utilizing an electrostatic spinning technology to obtain the chitosan derivative fiber membrane loaded with the capsaicin. The chitosan derivative fiber membrane loaded with capsaicin prepared by the invention has stronger tissue adhesive property and analgesic, anti-inflammatory and bacteriostatic effects, thereby improving the wound healing effect and being applicable to the fields of tissue repair technology and the like; simple operation, good stability and good application prospect.
Description
Technical Field
The invention relates to the technical field of preparation of tissue repair materials, in particular to a preparation method and application of a chitosan derivative fibrous membrane loaded with capsaicin.
Background
Chitosan is a natural polycation polysaccharide derivative, and has been applied in the medical field as a biological material due to good biocompatibility and biological functions such as antibacterial and antitumor activities. However, the chitosan is only dissolved in an acid solution, and the wider application of the chitosan is limited by the defects of insufficient tissue adhesion and the like. Therefore, many researchers have studied the preparation and application of chitosan derivatives.
Capsaicin has the effects of diminishing inflammation, easing pain, resisting bacteria and promoting fat metabolism, and is also widely applied to the fields of neuropathic pain, osteoarthritis, rheumatoid arthritis and the like. However, capsaicin is associated with side effects such as burning sensation when applied directly due to its irritancy.
In order to solve the problems, the invention utilizes chitosan and capsaicin to prepare the multifunctional fibrous membrane with the effects of bacteriostasis, inflammation diminishing, pain easing and the like, the chitosan is modified to have stronger tissue adhesive force, and the capsaicin is loaded to be slowly released so as to reduce the side effect of the chitosan, and the chitosan and capsaicin composite fibrous membrane is applied to medical fields of tissue adhesives (operation parts and the like), wound dressings (external wounds), adhesive bandage (arthritis and the like), and the like, so the chitosan and capsaicin composite fibrous membrane has good application value in clinical medicine.
Disclosure of Invention
The invention aims to provide a preparation method and application of a chitosan derivative fiber membrane loaded with capsaicin.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of a chitosan derivative fiber membrane carrying capsaicin comprises the following steps:
dissolving chitosan in distilled water, and adding a proper amount of hydrochloric acid and sodium hydroxide to adjust the pH of the solution to 6.3-6.5 to obtain a chitosan solution;
respectively dissolving 3, 4-dihydroxyphenyl propionic acid and 1-ethyl-3- (3-dimethyl aminopropyl) carbodiimide hydrochloride in alcohol, respectively adding the dissolved solution into the chitosan solution obtained in the step (1), reacting, dialyzing and separating to obtain a chitosan derivative, and freeze-drying and storing the chitosan derivative;
and (3) dissolving the chitosan derivative obtained in the step (2) in distilled water to obtain a modified chitosan derivative aqueous solution, adding capsaicin into the modified chitosan derivative aqueous solution, and reacting to obtain the capsaicin-loaded chitosan derivative. (ii) a
Preferably, step (4), the chitosan derivative loaded with capsaicin obtained in step (3) is subjected to a spinning process by using an electrostatic spinning technology; in the electrostatic spinning process, under the action of a high-voltage electric field between the injection head and the receiving screen, charges carried on the surfaces of the jet flows repel each other, so that the jet flows are dispersed, a plurality of fine fibers with similar diameters are formed and fall on the receiving screen, and the capsaicin-loaded chitosan derivative fiber membrane is obtained.
Preferably, in the step (1), the chitosan has a molecular weight of 250-300 kDa.
Preferably, in the step (2), 3, 4-dihydroxyphenyl propionic acid and 1-ethyl-3- (3-dimethyl aminopropyl) carbodiimide hydrochloride are respectively dissolved in alcohol at a molar ratio of 1:1, and after the dissolution, the solutions are respectively added into the chitosan solution obtained in the step (1) to react for 1.5 hours at 30 ℃.
Preferably, in the step (2), the modified chitosan derivative is obtained by dialysis separation, and is stored after being freeze-dried for 24-36 hours.
Preferably, in the step (3), the concentration of capsaicin is 0.001-0.08mg/ml, the reaction temperature for preparing the capsaicin-loaded chitosan derivative is 30 ℃, and the reaction time is 1-3 hours.
Preferably, in the step (2) and the step (3), the chitosan derivative is a catechol-modified chitosan derivative.
Preferably, the spinning solution with the concentration of 5-25mg/L is injected into an injector, electrostatic spinning is carried out under the temperature and humidity conditions that the ambient temperature is 20-25 ℃ and the humidity is 45-50%, the spinning voltage is set to be 10-25kV, the receiving distance is 12-20cm, and the spinning speed is 0.3-0.7ml/h, so that spinning is carried out.
The invention also provides application of the chitosan derivative fiber membrane carrying capsaicin in the aspect of tissue repair technology, and the slow release of the capsaicin on the chitosan derivative fiber membrane can play a role in relieving pain and diminishing inflammation, thereby achieving the effects of accelerating healing and relieving wound pain.
Compared with the prior art, the invention has the beneficial effects that:
1. the modified chitosan derivative has strong tissue adhesion and in-vivo biodegradability, and the preparation method is simple to operate, good in stability and good in application prospect.
2. The chitosan derivative fiber membrane loaded with capsaicin prepared by the spinning technology can play a role in relieving pain and diminishing inflammation through the slow release of the capsaicin on the chitosan derivative fiber membrane, thereby achieving the effects of accelerating healing and relieving wound pain.
3. The chitosan derivative fiber membrane loaded with capsaicin prepared by the invention is applied to the medical fields of wound dressings (external wounds), tissue adhesives (operation parts and the like), adhesive bandages (arthritis and the like) and the like by regulating and controlling the content of the capsaicin.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
a preparation method of a chitosan derivative fiber membrane carrying capsaicin comprises the following steps:
(1) a chitosan derivative: 1g of chitosan was dissolved in 100ml of distilled water, and an appropriate amount of hydrochloric acid and sodium hydroxide were added to adjust the pH of the solution to 6.3, to obtain a chitosan solution. Subsequently, 3, 4-dihydroxylpropyl acid and 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride-modified chitosan were dissolved in 50ml of ethanol at a molar ratio of 1:1, respectively, and then added to the chitosan solution to react at 30 ℃ for 1.5 hours, respectively. After dialysis, the prepared catechol-modified chitosan derivative is stored after being frozen and dried for 24 hours;
(2) chitosan derivative capsaicin-loaded spinning solution: dissolving the catechol modified chitosan prepared in the step (1) in distilled water, adding capsaicin to dissolve in a catechol chitosan solution to enable the concentration of the capsaicin to reach 0.05mg/ml and the concentration of a spinning solution to reach 5mg/L, and reacting at 30 ℃ for 1 hour to obtain a capsaicin-loaded chitosan derivative;
(3) fiber membrane: and (3) carrying out a spinning process on the capsaicin-loaded chitosan derivative obtained in the step (2) by utilizing an electrostatic spinning technology. And (3) injecting the spinning solution into an injector, and carrying out electrostatic spinning under the temperature and humidity conditions that the ambient temperature is 20 ℃ and the humidity is 45%, wherein the spinning voltage is 10kV, the receiving distance is 12cm, and the spinning speed is 0.3ml/h, so as to carry out spinning.
Example 2:
a preparation method of a chitosan derivative fiber membrane carrying capsaicin comprises the following steps:
(1) a chitosan derivative: 1g of chitosan was dissolved in 100ml of distilled water, and an appropriate amount of hydrochloric acid and sodium hydroxide were added to adjust the pH of the solution to 6.4, to obtain a chitosan solution. Subsequently, 3, 4-dihydroxylpropyl acid and 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride-modified chitosan were dissolved in 50ml of ethanol at a molar ratio of 1:1, respectively, and then added to the chitosan solution to react at 30 ℃ for 1.5 hours, respectively. After the dialysis process, the prepared catechol-modified chitosan derivative is stored after being frozen and dried for 30 hours;
(2) chitosan derivative capsaicin-loaded spinning solution: dissolving the catechol modified chitosan prepared in the step (1) in distilled water, adding capsaicin to dissolve in a catechol chitosan solution to enable the concentration of the capsaicin to reach 0.06mg/ml and the concentration of a spinning solution to reach 15mg/L, and reacting at 30 ℃ for 1 hour to obtain a capsaicin-loaded chitosan derivative;
(3) fiber membrane: and (3) carrying out a spinning process on the capsaicin-loaded chitosan derivative obtained in the step (2) by utilizing an electrostatic spinning technology. And (3) injecting the spinning solution into an injector, and carrying out electrostatic spinning under the temperature and humidity conditions that the ambient temperature is 23 ℃ and the humidity is 47%, wherein the spinning voltage is 20kV, the receiving distance is 18cm, and the spinning speed is 0.5ml/h, so as to carry out spinning.
Example 3:
a preparation method of a chitosan derivative fiber membrane carrying capsaicin comprises the following steps:
(1) a chitosan derivative: 1g of chitosan was dissolved in 100ml of distilled water, and an appropriate amount of hydrochloric acid and sodium hydroxide were added to adjust the pH of the solution to 6.5, to obtain a chitosan solution. Subsequently, 3, 4-dihydroxylpropyl acid and 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride-modified chitosan were dissolved in 50ml of ethanol at a molar ratio of 1:1, respectively, and then added to the chitosan solution to react at 30 ℃ for 1.5 hours, respectively. After dialysis, the prepared catechol-modified chitosan derivative is stored after being frozen and dried for 36 hours;
(2) chitosan derivative capsaicin-loaded spinning solution: dissolving the catechol modified chitosan prepared in the step (1) in distilled water, adding capsaicin to dissolve in a catechol chitosan solution to enable the concentration of the capsaicin to reach 0.08mg/ml and the concentration of a spinning solution to reach 25mg/L, and reacting for 1 hour at 30 ℃;
(3) fiber membrane: and (3) carrying out a spinning process on the capsaicin-loaded chitosan derivative obtained in the step (2) by utilizing an electrostatic spinning technology. And (3) injecting the spinning solution into an injector, and carrying out electrostatic spinning under the temperature and humidity conditions that the ambient temperature is 25 ℃ and the humidity is 50%, wherein the spinning voltage is 25kV, the receiving distance is 20cm, and the spinning speed is 0.7ml/h, so as to carry out spinning.
And (3) performance detection:
the detection of antibacterial performance, cytotoxicity and tissue repair performance of the chitosan derivative fiber membranes loaded with capsaicin obtained in the above example 1, example 2 and example 3 specifically comprises the following steps:
soaking the fibrous membrane in a cell culture medium, inoculating bacteria, culturing in a shaking table at 37 ℃, and taking out after 24 hours to measure the antibacterial performance; soaking the fibrous membrane in a cell culture medium, culturing for 24 hours in a constant-temperature cell culture box at 37 ℃, and taking out to measure cytotoxicity; a round wound with a diameter of 0.7mm was made on the back of the mouse, and the prepared chitosan derivative fiber membrane loaded with capsaicin was stuck to the wound to observe the degree of wound healing. The test results showed that the chitosan derivative fiber film loaded with capsaicin prepared by the example of the present invention has the effect of accelerating wound healing, compared to the chitosan derivative not loaded with capsaicin.
The application field is as follows: the chitosan derivative fiber membrane loaded with capsaicin prepared by the invention is applied to the medical fields of tissue adhesives (operation parts and the like), wound dressings (external wounds), adhesive bandages (arthritis and the like) and the like by regulating and controlling the content of the capsaicin.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A preparation method of a chitosan derivative fiber membrane carrying capsaicin is characterized by comprising the following steps: the method comprises the following steps:
dissolving chitosan in distilled water, and adding a proper amount of hydrochloric acid and sodium hydroxide to adjust the pH of the solution to 6.3-6.5 to obtain a chitosan solution;
respectively dissolving 3, 4-dihydroxyphenyl propionic acid and 1-ethyl-3- (3-dimethyl aminopropyl) carbodiimide hydrochloride in alcohol, respectively adding the dissolved solution into the chitosan solution obtained in the step (1), reacting, dialyzing and separating to obtain a chitosan derivative, and freeze-drying and storing the chitosan derivative;
step (3), dissolving the chitosan derivative obtained in the step (2) in distilled water to obtain a modified chitosan derivative aqueous solution, adding capsaicin into the modified chitosan derivative aqueous solution, and reacting to obtain a capsaicin-loaded chitosan derivative;
step (4), carrying out a spinning process on the capsaicin-loaded chitosan derivative obtained in the step (3) by using an electrostatic spinning technology; in the electrostatic spinning process, under the action of a high-voltage electric field between the injection head and the receiving screen, charges carried on the surfaces of the jet flows repel each other, so that the jet flows are dispersed, a plurality of fine fibers with similar diameters are formed and fall on the receiving screen, and the capsaicin-loaded chitosan derivative fiber membrane is obtained.
2. The method for preparing a capsaicin-loaded chitosan derivative fiber membrane according to claim 1, wherein: in the step (1), the molecular weight of the chitosan is 250-300 kDa.
3. The method for preparing a capsaicin-loaded chitosan derivative fiber membrane according to claim 1, wherein: in the step (2), 3, 4-dihydroxyphenyl propionic acid and 1-ethyl-3- (3-dimethyl aminopropyl) carbodiimide hydrochloride are respectively dissolved in alcohol according to the molar ratio of 1:1, and after the dissolution, the solutions are respectively added into the chitosan solution obtained in the step (1) to react for 1.5 hours at 30 ℃.
4. The method for preparing a capsaicin-loaded chitosan derivative fiber membrane according to claim 1, wherein: in the step (2), the modified chitosan derivative is obtained through dialysis separation, and is stored after being frozen and dried for 24-36 hours.
5. The method for preparing a capsaicin-loaded chitosan derivative fiber membrane according to claim 1, wherein: in the step (3), the concentration of capsaicin is 0.001-0.08mg/ml, the reaction temperature for preparing the chitosan derivative carrying capsaicin is 30 ℃, and the reaction time is 1-3 hours.
6. The method for preparing a capsaicin-loaded chitosan derivative fiber membrane according to claim 1, wherein: in the step (2) and the step (3), the chitosan derivative is catechol-modified chitosan derivative.
7. The method for preparing a capsaicin-loaded chitosan derivative fiber membrane according to claim 1, wherein: in the step (4), the spinning solution with the concentration of 5-25mg/L is injected into an injector, electrostatic spinning is carried out under the temperature and humidity conditions that the ambient temperature is 20-25 ℃ and the humidity is 45-50%, the spinning voltage is set to be 10-25kV, the receiving distance is 12-20cm, and the spinning speed is 0.3-0.7ml/h, so that spinning is carried out.
8. Use of a chitosan derivative fibre membrane loaded with capsaicin for use in tissue repair techniques according to any one of claims 1-7, wherein the slow release of capsaicin from the chitosan derivative fibre membrane is used to achieve pain and inflammation relief, thereby accelerating healing and alleviating pain in wounds.
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| CN115887778A (en) * | 2022-11-09 | 2023-04-04 | 南通大学 | Preparation method and application of antithrombotic thrombolytic small-caliber artificial blood vessel |
| CN116059437A (en) * | 2023-02-27 | 2023-05-05 | 南通大学 | Preparation method and application of anti-shrinkage blood contact tissue adhesive material |
| CN116059437B (en) * | 2023-02-27 | 2023-12-19 | 南通大学 | Preparation method and application of anti-shrinkage blood contact tissue adhesive material |
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