CN112370404B - Moisturizing and whitening composition, and preparation method and application thereof - Google Patents
Moisturizing and whitening composition, and preparation method and application thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The invention discloses a moisturizing and whitening composition, a preparation method and application thereof, and belongs to the technical field of cosmetics. The moisturizing and whitening composition is prepared from the following raw materials in parts by weight: 15-25 parts of vitis amurensis extract, 6-10 parts of medlar extract, 5-8 parts of neroli essential oil, 6-10 parts of liquorice extract, 2-6 parts of ginseng extract, 8-12 parts of tremella extract and 8-15 parts of aloe extract. The invention also discloses a preparation method and application of the moisturizing and whitening composition. The moisturizing and whitening composition can effectively inhibit tyrosinase activity and remove hydroxyl radicals; secondly, the product has good effects of oxidation resistance, allergy resistance, inflammation diminishing and itching relieving, and can obviously reduce anaphylactic reaction; thirdly, the skin-tendering, whitening, wrinkle-removing and freckle-removing functions are achieved; fourthly, the medicine has the effects of dredging skin capillaries and promoting blood circulation.
Description
Technical Field
The invention relates to a moisturizing and whitening composition, a preparation method and application thereof, and belongs to the technical field of cosmetics.
Background
The skin plays a barrier role in the human body, can protect the human body from external factors, and can prevent excessive water from losing from the body through the skin. With age, the skin becomes increasingly dry, wrinkled and flaccid, taking on an aged appearance. The gradual reduction of the water content of the skin is one of the pathological mechanisms of the aging appearance, and when the water content of the horny layer is lower than 10%, the skin is dry and desquamated, and the imbrication of the parallel arrangement of the horny cell layers occurs. The skin is easily eroded by the physical factors of the external environment, and water and electrolyte in the skin are also easily evaporated or lost. Skin moisturizing is the most important basic means for ensuring the barrier function of the stratum corneum and skin care and beauty.
Sensitive skin generally exists in common skin troubles, has complex causes, and can be a comprehensive phenomenon caused by the single or combined action of endogenous factors, exogenous factors and the like of the body. Although the exact definition of sensitive skin is not yet agreed upon, the clinical symptoms and signs of the skin are gradually described, i.e. discomfort, which is almost always manifested in the face of most patients, including subjective sensory symptoms such as burning, stinging, itching, tingling, which may or may not be accompanied by erythema. Sensitive skin, although not a skin disease, causes skin discomfort that directly affects the quality of life of people. Many people choose soothing sprays to relieve the symptoms of skin irritation whenever season changes or other sensitizing factors cause skin irritation. Most of the relieving sprays sold in the market at present directly act on the surface layer of the skin by utilizing the moisture in the spray to temporarily supplement the moisture to the skin, so that the allergy symptom of the surface layer can be relieved in a short time, but the sensitive skin is not fundamentally changed, and when the sensitive skin is stimulated by the external environment again, the sensitive skin still can be allergic, the face is red, swollen, itchy and painful, red blood streak or red pimples exist, and the anti-allergy skin relieving effect is not achieved.
At present, many beauty and skin care products take chemically synthesized substances (such as chemical preservatives, antioxidants and the like) as main raw materials, and most sprays contain chemical components such as alcohol, perfume, mineral oil and the like, so that the sprays can irritate the skin after being used for a long time, cause skin pigmentation and cause allergic contact dermatitis. Some women with oriental yellow race can achieve the effects of quickly whitening, removing freckles and the like, and even some heavy metal salt components can be added, the chemical additives can generate certain toxic and side effects on human skin more or less, and the chemical additives can easily cause health hidden troubles to human bodies after long-term use.
Therefore, the development of a pure natural moisturizing spray with the characteristics of pure nature, no side effect, no additive and the like is a problem to be solved urgently by the technical personnel in the field.
Disclosure of Invention
One of the objects of the present invention is to provide a moisturizing and whitening composition. The moisturizing and whitening composition can effectively inhibit tyrosinase activity and remove hydroxyl free radicals; secondly, the toothpaste has good effects of oxidation resistance, allergy resistance, inflammation diminishing and itching relieving, and can obviously reduce anaphylactic reaction; thirdly, the skin-tendering, whitening, wrinkle-removing and freckle-removing functions are achieved; fourthly, the medicine has the effects of dredging skin capillaries and promoting blood circulation.
The scheme for solving the technical problems is as follows: the moisturizing and whitening composition is prepared from the following raw materials in parts by weight: 15-25 parts of vitis amurensis extract, 6-10 parts of medlar extract, 5-8 parts of neroli essential oil, 6-10 parts of liquorice extract, 2-6 parts of ginseng extract, 8-12 parts of tremella extract and 8-15 parts of aloe extract.
The principle of the moisturizing and whitening composition of the invention is as follows:
1. sea grape extract: the sea grape is an edible green algae with rich nutrition, 80% of the sea grape is composed of algal polysaccharide, collagen and dietary fiber, the amino acid composition is balanced and complete in variety, the fat content is lower and is mostly unsaturated fatty acid, the mineral substance and vitamin content are rich, and the sea grape has the effects of resisting oxidation, cancer, tumors, purifying blood, diminishing inflammation, sterilizing, resisting ultraviolet rays, resisting aging and the like, beautifying and building the body. The composition of the marine grape fatty acid contains 14 kinds of fatty acids, the unsaturated fatty acid accounts for 70.87 percent of the total amount of the fatty acids, the linoleic acid accounts for 13.14 percent of the total amount of the fatty acids, the fatty acids are essential fatty acids for human bodies, and the deficiency of the fatty acids can cause rough skin, the reduction of tissue regeneration capability, the increase of infection symptoms of skin diseases and the like. The active components in the Vitis amurensis extract have antioxidant, antiaging and antiallergic effects.
The Vitis heyneana extract can be purchased commercially, such as from West Anshi Hao Biotech limited.
2. Fructus Lycii extract is obtained from the medicinal part of fructus Lycii, such as fructus Lycii. The medlar is rich in moisturizing factors, the moisturizing factors are a novel moisturizing agent with quick absorption and high activity, and can rapidly improve the moisture retention of skin and hair in the application of personal care products, stimulate cell activity, and have the effects of maintaining skin moist and smooth and preventing skin from being dry and dark.
The above extract of Lycium barbarum can be purchased commercially, for example, from Saikonia Biotechnology Co.
3. Orange flower essential oil: is extracted from bitter orange white petal, and has effects of sterilizing, improving cell activity, deodorizing, softening skin, whitening skin, keeping moisture and removing speckle. Is suitable for dry, sensitive and mature skin, especially spiral static scar and striae gravidarum, and is helpful for preventing ultraviolet, and can be used for protecting skin when X-ray is irradiated. Simultaneously, the medicine also has the function of calming parasympathetic nerves, can treat insomnia, and improve neuralgia, headache and dizziness.
The above neroli essential oil is commercially available, for example, from Shanghai Yangxin Biotech, Inc.
4. And (3) liquorice extract: is extracted from Glycyrrhrizae radix, and contains triterpene saponin and flavone glycoside as main active components 10-30% and 25-80%. The flavone glycoside has effects of inhibiting activity of tyrosinase, scavenging oxygen free radicals, resisting inflammation, and resisting allergy, so as to remove yellow and whiten skin.
The above licorice extract can be purchased commercially, for example, from Sai' oreli bioengineering Co.
5. Ginseng root extract: is extracted from Ginseng radix, and has effects of nourishing and resisting aging, relieving skin, and whitening skin. For skin, ginseng contains ginsenoside, so that blood circulation can be promoted, skin aging and wrinkle generation can be prevented, nutrition supply of skin can be increased, effects of tonifying qi, beautifying and activating blood can be achieved, immunity can be improved, and skin can be prevented from being harmfully irritated by external environment. Can accelerate metabolism, deeply moisten skin, prevent dryness and aging, and effectively treat pruritus and desquamation to make facial skin fine and smooth. The skin is pure white and tender.
The ginseng root extract can be purchased commercially, for example, from Shanxi Huidonda Biotech Co., Ltd.
6. White fungus extract: is extracted from Tremella. The tremella contains tremella polysaccharide ingredients, the tremella polysaccharide can absorb a large amount of water, and has the same high moisture retention property no matter under the conditions of low humidity or high humidity, and the tremella is used in skin care products, has the functions of repairing epidermis and increasing the water content of the epidermis, improves the water content of stratum corneum, gives the skin a soft feeling, improves the integral value of the skin conductivity, improves the water retention capacity of the skin, and reduces the water release constant; the tremella polysaccharide has no sticky feeling, can not feel tight after being dried, can provide moist and smooth feeling for skin, can resist partial free radicals, and is particularly suitable for skin care products.
The above Tremella extract can be purchased commercially, such as from Sai' an Youchuo Biotech Co.
7. Aloe extract: is extracted from Aloe. The Aloe is juice concentrated dried product of leaves of Aloe barbadensis Miller, Aloe ferox Miller or other similar plants of Liliaceae. Modern researches prove that aloe has the effects of resisting cancer, inflammation and virus, resisting bacteria and killing pests, relieving fever and protecting liver, enhancing immunity and the like, is widely applied to various fields of medicine industry, daily chemical industry, beauty cosmetics, food health care and the like, and is a medicinal plant with economic value. Anthraquinone and aloe polysaccharide are main effective components in aloe, and have effects of diminishing inflammation, resisting virus, purgation, resisting cancer, resisting aging, caring skin, etc.
The above aloe extract can be purchased commercially, for example, from Shanxi Rick Biotech Co., Ltd.
In the prior art and in the research process of the inventor, various traditional Chinese medicine extracts are found to have the capability of eliminating superoxide anion free radicals, such as medlar, iris, willow flower, codonopsis pilosula and the like. Then when the traditional Chinese medicine extracts are independently applied, the effect of eliminating superoxide anion free radicals is not obvious, and the requirement of anti-aging is difficult to meet. Therefore, in the prior art, compounding of various traditional Chinese medicine extracts is attempted to improve the effect of removing superoxide anion free radicals, however, the traditional Chinese medicine extracts are complex in components, and after some traditional Chinese medicine extracts are compounded, the effect of removing superoxide anion free radicals is not enhanced but reduced; or the effect is not enhanced. After screening a large number of compound formulas, the invention surprisingly and unexpectedly discovers that even under the condition that the dosage is the same as that of a single component, after the sea grape extract, the medlar extract, the neroli essential oil, the liquorice extract, the ginseng extract, the tremella extract and the aloe extract are compounded, the effect of obviously enhancing the removal of superoxide anion free radicals can be obtained, and the seven components have synergistic effects. The traditional Chinese medicine extract is practically applied to various cosmetics such as essence, and the like, and can also play a remarkable anti-aging effect. In addition, many traditional Chinese medicine extracts in the prior art have irritation, but experiments prove that the composition of the sea grape extract, the Chinese wolfberry extract, the neroli essential oil, the liquorice extract, the ginseng extract, the tremella extract and the aloe extract has no irritation.
The storage method of the moisturizing and whitening composition of the invention comprises the following steps: storing in dark at the temperature of less than or equal to 25 ℃.
The moisturizing and whitening composition has the beneficial effects that:
1. the moisturizing and whitening composition can effectively inhibit tyrosinase activity and remove hydroxyl free radicals; secondly, the toothpaste has good effects of oxidation resistance, allergy resistance, inflammation diminishing and itching relieving, and can obviously reduce anaphylactic reaction; thirdly, the skin-tendering, whitening, wrinkle-removing and freckle-removing functions are achieved; fourthly, the medicine has the effects of dredging skin capillary vessels and promoting blood circulation.
2. The moisturizing and whitening composition can be used for preparing various types of cosmetics, can emit natural fragrance, has no toxic or side effect, and is safe and reliable.
On the basis of the technical scheme, the invention can be further improved as follows.
Further, the feed is prepared from the following raw materials in parts by weight: 15 parts of sea grape extract, 10 parts of medlar extract, 5 parts of neroli essential oil, 10 parts of liquorice extract, 2 parts of ginseng extract, 12 parts of tremella extract and 8 parts of aloe extract.
The adoption of the further beneficial effects is as follows: the above are the optimal parameters, and the efficacy of the moisturizing and whitening composition is optimal.
Further, the feed is prepared from the following raw materials in parts by weight: 20 parts of sea grape extract, 8 parts of medlar extract, 6.5 parts of neroli essential oil, 8 parts of liquorice extract, 4 parts of ginseng extract, 10 parts of tremella extract and 10.5 parts of aloe extract.
The adoption of the further beneficial effects is as follows: the above parameters are optimal parameters, and the obtained moisturizing and whitening composition has optimal efficacy.
Further, the feed is prepared from the following raw materials in parts by weight: 25 parts of sea grape extract, 6 parts of medlar extract, 8 parts of neroli essential oil, 6 parts of liquorice extract, 6 parts of ginseng extract, 8 parts of tremella extract and 15 parts of aloe extract.
The adoption of the further beneficial effects is as follows: the above parameters are optimal parameters, and the obtained moisturizing and whitening composition has optimal efficacy.
The second object of the present invention is to provide a method for preparing the moisturizing and whitening composition. The moisturizing and whitening composition disclosed by the invention is simple in preparation method, easy to operate, wide in market prospect and suitable for large-scale popularization and application.
The scheme for solving the technical problems is as follows: a preparation method of a moisturizing and whitening composition comprises the following steps:
taking the following raw materials in parts by weight: 15-25 parts of vitis amurensis extract, 6-10 parts of medlar extract, 5-8 parts of neroli essential oil, 6-10 parts of liquorice extract, 2-6 parts of ginseng extract, 8-12 parts of tremella extract and 8-15 parts of aloe extract, and stirring and mixing uniformly at room temperature to obtain the moisturizing and whitening composition.
On the basis of the technical scheme, the invention can be further improved as follows.
Further, the stirring speed is 600r/min-1000r/min, and the time is 60min-100 min.
The adoption of the further beneficial effects is as follows: with the above parameters, the mixing is more uniform.
The invention also provides application of the moisturizing and whitening composition. The moisturizing and whitening composition can be used for preparing various types of cosmetics, and consumers can make flexible selection according to needs, so that the moisturizing and whitening composition has a wide market prospect.
The scheme for solving the technical problems is as follows: the application of the moisturizing and whitening composition in preparing cosmetics.
The application of the moisturizing and whitening composition in the preparation of cosmetics has the beneficial effects that:
the moisturizing and whitening composition can be used for preparing various types of cosmetics, and consumers can make flexible selection according to needs, so that the moisturizing and whitening composition has a wide market prospect.
On the basis of the technical scheme, the invention can be further improved as follows.
Further, the cosmetic is any one of essence, skin cream, lotion, gel, toner, spray water, eye patch and facial mask.
Furthermore, the essence is prepared from the moisturizing and whitening composition and mineral water according to the mass ratio of 1 (0.5-5).
The adoption of the further beneficial effects is as follows: the essence can be obtained by adopting the proportion.
Furthermore, the preparation method of the essence comprises the following steps: respectively weighing the moisturizing and whitening composition and the mineral water according to the mass ratio of 1 (0.5-5), mixing, stirring for 60min-120min at the temperature of 30-90 ℃ and at the rotating speed of 600-1000 r/min, and naturally cooling to room temperature to obtain the essence.
The adoption of the further beneficial effects is as follows: by adopting the method, the essence can be prepared.
Furthermore, the skin cream is prepared from the moisturizing and whitening composition and glycerol according to the mass ratio of 1 (0.5-10).
The adoption of the further beneficial effects is as follows: by adopting the proportion, the skin cream can be obtained.
Furthermore, the toner is prepared from the moisturizing and whitening composition and mineral water according to the mass ratio of 1 (11-500).
The adoption of the further beneficial effects is as follows: the skin cream can be obtained by adopting the toner.
Furthermore, the facial mask is prepared by coating the moisturizing and whitening composition on facial mask paper or facial mask cloth.
The adoption of the further beneficial effects is as follows: by adopting the toner, a facial mask can be obtained.
Detailed Description
The principles and features of this invention are described below in conjunction with specific embodiments, which are set forth merely to illustrate the invention and are not intended to limit the scope of the invention.
Example 1
The moisturizing and whitening composition is prepared from the following raw materials in parts by weight: 15 parts of sea grape extract, 10 parts of medlar extract, 5 parts of neroli essential oil, 10 parts of liquorice extract, 2 parts of ginseng extract, 12 parts of tremella extract and 8 parts of aloe extract.
The preparation method of the moisturizing and whitening composition comprises the following steps:
taking the following raw materials in parts by weight: 15 parts of sea grape extract, 10 parts of medlar extract, 5 parts of neroli essential oil, 10 parts of liquorice extract, 2 parts of ginseng extract, 12 parts of tremella extract and 8 parts of aloe extract, stirring for 100min at the rotating speed of 600r/min at room temperature, and uniformly mixing to obtain the moisturizing and whitening composition.
The application of the moisturizing and whitening composition in preparing cosmetics.
The cosmetic is any one of essence, skin cream, lotion, gel, toner, spray water, eye patch and facial mask.
The essence consists of the moisturizing and whitening composition and mineral water according to the mass ratio of 1: 0.5.
The preparation method of the essence comprises the following steps: respectively weighing the moisturizing and whitening composition and the mineral water according to the mass ratio of 1:0.5, mixing, stirring for 120min at the temperature of 30 ℃ and the rotation speed of 600r/min, and naturally cooling to room temperature to obtain the essence.
The skin cream is prepared from the moisturizing and whitening composition and glycerin according to the mass ratio of 1: 0.5.
The toner is prepared from the moisturizing and whitening composition and mineral water according to the mass ratio of 1: 11.
The facial mask is prepared by coating the moisturizing and whitening composition on facial mask paper or facial mask cloth.
Example 2
The moisturizing and whitening composition is prepared from the following raw materials in parts by weight: 20 parts of sea grape extract, 8 parts of medlar extract, 6.5 parts of neroli essential oil, 8 parts of liquorice extract, 4 parts of ginseng extract, 10 parts of tremella extract and 10.5 parts of aloe extract.
The preparation method of the moisturizing and whitening composition comprises the following steps:
taking the following raw materials in parts by weight: 15 parts of sea grape extract, 10 parts of medlar extract, 5 parts of neroli essential oil, 10 parts of liquorice extract, 2 parts of ginseng extract, 12 parts of tremella extract and 8 parts of aloe extract, stirring for 80min at the rotating speed of 800r/min at room temperature, and uniformly mixing to obtain the moisturizing and whitening composition.
The application of the moisturizing and whitening composition in preparing cosmetics.
The cosmetic is any one of essence, skin cream, lotion, gel, toner, spray water, eye patch and facial mask.
The essence consists of the moisturizing and whitening composition and mineral water according to a mass ratio of 1: 2.
The preparation method of the essence comprises the following steps: respectively weighing the moisturizing and whitening composition and the mineral water according to the mass ratio of 1:2, mixing, stirring for 90min at the temperature of 60 ℃ and the rotation speed of 800r/min, and naturally cooling to room temperature to obtain the essence.
The skin cream is prepared from the moisturizing and whitening composition and glycerin according to the mass ratio of 1: 5.
The toner is prepared from the moisturizing and whitening composition and mineral water according to the mass ratio of 1: 250.
The facial mask is prepared by coating the moisturizing and whitening composition on facial mask paper or facial mask cloth.
Example 3
The moisturizing and whitening composition is prepared from the following raw materials in parts by weight: 25 parts of sea grape extract, 6 parts of medlar extract, 8 parts of neroli essential oil, 6 parts of liquorice extract, 6 parts of ginseng extract, 8 parts of tremella extract and 15 parts of aloe extract.
The preparation method of the moisturizing and whitening composition comprises the following steps:
taking the following raw materials in parts by weight: 15 parts of sea grape extract, 10 parts of medlar extract, 5 parts of neroli essential oil, 10 parts of liquorice extract, 2 parts of ginseng extract, 12 parts of tremella extract and 8 parts of aloe extract, stirring for 60min at the rotation speed of 1000r/min at room temperature, and uniformly mixing to obtain the moisturizing and whitening composition.
The application of the moisturizing and whitening composition in preparing cosmetics.
The cosmetic is any one of essence, skin cream, lotion, gel, toner, spray water, eye patch and facial mask.
The essence consists of the moisturizing and whitening composition and mineral water according to a mass ratio of 1: 5.
The preparation method of the essence comprises the following steps: respectively weighing the moisturizing and whitening composition and the mineral water according to the mass ratio of 1:5, mixing, stirring for 60min at the temperature of 90 ℃ and the rotation speed of 1000r/min, and naturally cooling to room temperature to obtain the essence.
The skin cream is prepared from the moisturizing and whitening composition and glycerin according to the mass ratio of 1: 10.
The toner is prepared from the moisturizing and whitening composition and mineral water according to the mass ratio of 1: 500.
The facial mask is prepared by coating the moisturizing and whitening composition on facial mask paper or facial mask cloth.
Comparative example 1
The difference from example 2 is that the same as in the case of the comparative example except that the sea grape extract was not added to the raw material. Specifically, the composition of the comparative example is prepared from the following raw materials in parts by weight: 8 parts of medlar extract, 6.5 parts of neroli essential oil, 8 parts of liquorice extract, 4 parts of ginseng extract, 30 parts of tremella extract and 10.5 parts of aloe extract.
The preparation of the above composition is the same as in example 2.
The essence of the comparative example consists of the composition and mineral water according to the mass ratio of 1: 2.
The essence of this comparative example was prepared as described in example 2.
Comparative example 2
The difference from example 2 is that the raw materials of the comparative example are not added with the tremella extract, and the rest is the same. Specifically, the composition of the comparative example is prepared from the following raw materials in parts by weight: 30 parts of sea grape extract, 8 parts of medlar extract, 6.5 parts of neroli essential oil, 8 parts of liquorice extract, 4 parts of ginseng extract and 10.5 parts of aloe extract.
The preparation of the above composition is the same as in example 2.
The essence of the comparative example consists of the composition and mineral water according to the mass ratio of 1: 2.
The preparation method of the essence of the comparative example is the same as that of example 2.
Comparative example 3
The difference from example 2 is that the raw materials of the comparative example are not added with the sea grape extract and the tremella extract, and the rest is the same. Specifically, the composition of the comparative example is prepared from the following raw materials in parts by weight: 14 parts of medlar extract, 12.5 parts of neroli essential oil, 14 parts of liquorice extract, 10 parts of ginseng extract and 16.5 parts of aloe extract.
The preparation of the above composition is the same as in example 2.
The essence of the comparative example consists of the composition and mineral water according to the mass ratio of 1: 2.
The preparation method of the essence of the comparative example is the same as that of example 2.
Experimental example 1: tyrosinase inhibitory Activity test
The experimental methods are described in the references [1] . 10ml of each of the essences prepared in examples 1 to 3 and comparative examples 1 to 3 was taken. The samples were dissolved in phosphate buffer (pH 6.8) and each sample was prepared to be tested at a concentration of 1 mg/ml.
Tyrosinase (100U) was added to 2ml of each test group (the blank group was replaced with phosphate buffer), heated in a 37 ℃ constant temperature water bath for 10min, and then 1ml of 0.003mol/L dopa reaction solution was added thereto, and after stirring sufficiently, the reaction mixture was reacted at 37 ℃ (constant temperature water bath) for 2min, immediately taken out, and the absorbance at 475nm was measured.
Inhibition { [ (a-B) - (C-D) ]/(a-B) } × 100%.
A is the absorbance measured at 475nm of the mixed solution of the non-loaded sample and the tyrosinase; b is the absorbance measured at 475nm of the mixed solution without the sample and the tyrosinase; c is the absorbance measured at 475nm of the mixed solution of the sample and the tyrosinase; d is the absorbance at 475nm of the mixture with sample and without tyrosinase. The results are shown in Table 1.
TABLE 1 inhibition of tyrosinase
| Group of | Inhibition ratio (%) |
| Example 1 | 76.8 |
| Example 2 | 79.6 |
| Example 3 | 77.2 |
| Comparative example 1 | 40 |
| Comparative example 2 | 48 |
| Comparative example 3 | 37 |
As can be seen from table 1, the inhibition ratios of the essences prepared in examples 1 to 3 to tyrosinase were 76.8% to 79.6%, and the inhibition ratios of the essences prepared in comparative examples 1 to 3 to tyrosinase were 40% to 48%, which indicates that the whitening effects of the essences prepared in examples 1 to 3 are significantly higher than those of the essences prepared in comparative examples 1 to 3. Therefore, the moisturizing and whitening composition can effectively inhibit the activity of tyrosinase and remove hydroxyl free radicals, and has good antioxidation.
Experimental example 2: performance testing of products
1 test subject and method
1.1 test subject
1.1.1 inclusion criteria for subjects: (1) normal male and female subjects 18-60 years of age, female first; (2) those without severe systemic disease, immunodeficiency or autoimmune disease; (3) those with inactive allergic disease; (4) the past has no history of allergy to skin care cosmetics; (5) in the last month, hormone drugs and immunosuppressants have not been used systemically; (6) no other clinical trial participants; (7) volunteers participate and can complete the specified contents according to the test requirements.
1.1.2 exclusion conditions: (1) pregnant or lactating women; (2) outdoor work is the main, and sun exposure cannot be avoided; (3) those with increased exposure to sunlight during the test period for various reasons (e.g., travel, vacation); (4) during the trial period, the hormone and immune preparation medicines are applied to the whole body; (5) those who apply anti-inflammatory drugs to their face during trial; (6) the subject is not used as intended or the data is not complete.
1.1.3 number of subjects: and the number of the subjects is 35 according to the guidelines for evaluating the moisturizing effect of cosmetics and the guidelines for evaluating the efficacy of whitening cosmetics.
1.2 methods
1.2.1 preparation of test samples: in order to avoid the contamination of the sample by microorganisms during the preparation process, scissors, packaging bags and the like which are in direct contact with the essence are sterilized before use, and the person preparing the sample wears gloves for operation.
1.2.2 facial test sites: forehead, chin, left cheek, right cheek as test area.
1.2.3 face test method: whitening products (cosmetics, external medicines or oral health products) cannot be used 2-3 days before the face test. Before the test, the test subjects need to uniformly clean the facial skin and wipe the skin clean with dry facial tissues. The subject was allowed to sit still in a standard room for at least 30min with facial skin exposed, unable to rub, and kept relaxed.
1.2.4 testing aging: periodic efficacy assessments, 3 times per week, were tested for 12 consecutive weeks.
1.2.5 evaluation: the trial effect of the essence of example 2 was evaluated by means of a questionnaire, wherein the moisture retention was evaluated by a skin moisture tester, and the appearance and whitening and brightening effects were evaluated by the subject's sense; 2ml of essence is uniformly coated.
Table 2 sensory evaluation of mask
1.3 results of the experiment
Sensory testing is carried out on 50 qualified subjects, 2 persons quit in the process of sensory testing for different reasons, 48 complete testing results are finally obtained, the testing results are divided into three grades of general, satisfied and very satisfied, the number of persons in each grade under each testing item is counted, and corresponding testing results are obtained, and the whole testing results are shown in the following table:
table 3 sensory evaluation results table of the essence obtained in example 2
Second, moisture retention test of facial mask measured by skin moisture tester
2.1 Experimental instruments
Digital skin tester.
2.2 principle
The moisture content of the skin is determined by two factors, internal and external, the stratum corneum of the skin has a greatly varying capacity to retain water, and the moisture content can vary from 10% to 60%, and is primarily the composition of the respiratory process of the skin's perspiration and the water mixture in the skin. External factors including ambient temperature, humidity, drugs and cosmetics, etc., can determine and change the moisture content of the skin. The moisture content of the skin affects the formation of a water and oil mixed film on the skin surface, and this protective film is very important to the aging of the skin. It is therefore useful to quantitatively test the moisture content of the skin and the amount of change after a certain period of care.
2.3 Experimental methods
Powerful products (cosmetics, external medicines or internal health care products) cannot be used 2 to 3 days before the face test. Before the test, the test subjects need to uniformly clean the facial skin and wipe the skin clean with dry facial tissues. The subject was allowed to sit still in a standard room for at least 30min with facial skin exposed, unable to rub, and kept relaxed. The moisture on the forehead and cheeks of the skin was measured using a skin moisture meter, and the skin moisture before the test was read and recorded.
Applying 2ml of essence, taking down after 15min, and beating until absorption. The essence solutions were obtained in example 2 and comparative examples 1 to 3, respectively. Without any facial product used for 12 hours, the test was performed after 10min, 30min, 60min and 12h, respectively, and the skin moisture after the test was recorded.
The water content increase value of the facial skin after the product was used was determined by an average increase value method (increase value: skin water after the test — skin water before the test).
2.4 results of the experiment
As shown in tables 4-7.
Table 4 essence obtained in example 2
Table 5 essence obtained in comparative example 1
Table 6 essence obtained in comparative example 2
Table 7 essence obtained in comparative example 3
As can be seen from the comparison of tables 4 and 5, in the essence of comparative example 1, the effects of oxidation resistance, aging resistance and allergy inhibition were weak because the extract of the vitis amurensis was not added.
As can be seen from comparison between tables 4 and 6, the essence of comparative example 2 has weak moisture retention and antioxidant effects because no tremella extract is added.
As can be seen from the comparison between tables 4 and 7, the essence of comparative example 3 has weak effects of oxidation resistance, aging resistance, allergy inhibition and moisture retention because the vitis amurensis extract and the tremella fuciformis extract are not added.
In conclusion, the moisturizing and whitening composition can effectively inhibit tyrosinase activity and remove hydroxyl free radicals; secondly, the toothpaste has good effects of oxidation resistance, allergy resistance, inflammation diminishing and itching relieving, and can obviously reduce anaphylactic reaction; thirdly, the skin-tendering, whitening, wrinkle-removing and freckle-removing functions are achieved; fourthly, the medicine has the effects of dredging skin capillaries and promoting blood circulation.
Reference documents:
1. wei Zheng, Sun Ling, Zhang Yan, etc. Studies on the inhibition of tyrosinase by soybean isoflavone [ J ]. Guangzhou food industry science and technology, 2004,20(3): 10-11.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (7)
1. The moisturizing and whitening composition is characterized by being prepared from the following raw materials in parts by weight: 20 parts of sea grape extract, 8 parts of medlar extract, 6.5 parts of neroli essential oil, 8 parts of liquorice extract, 4 parts of ginseng extract, 10 parts of tremella extract and 10.5 parts of aloe extract.
2. A preparation method of a moisturizing and whitening composition is characterized by comprising the following steps:
taking the following raw materials in parts by weight: 20 parts of sea grape extract, 8 parts of medlar extract, 6.5 parts of neroli essential oil, 8 parts of liquorice extract, 4 parts of ginseng extract, 10 parts of tremella extract and 10.5 parts of aloe extract, and stirring and mixing the components uniformly at room temperature to obtain the moisturizing and whitening composition.
3. The method for preparing the moisturizing and whitening composition according to claim 2, wherein the stirring speed is 600r/min to 1000r/min, and the time is 60min to 100 min.
4. Use of the moisturizing and whitening composition of claim 1 in the preparation of cosmetics.
5. The use according to claim 4, wherein the cosmetic is any one of essence, skin cream, lotion, gel, toner, spray water, eye mask and pack.
6. The use of claim 5, wherein the essence is prepared from the moisturizing and whitening composition of claim 1 and mineral water in a mass ratio of 1 (0.5-5); the skin cream is prepared from the moisturizing and whitening composition as claimed in claim 1 and glycerin according to a mass ratio of 1 (0.5-10); the toner is prepared from the moisturizing and whitening composition as claimed in claim 1 and mineral water according to a mass ratio of 1 (11-500); the mask is prepared by coating the moisturizing and whitening composition of claim 1 on mask paper or mask cloth.
7. The use according to claim 6, characterized in that the essence is prepared by a method comprising: respectively weighing the moisturizing and whitening composition and the mineral water according to the mass ratio of 1 (0.5-5), mixing, and rotating at the temperature of 30-90 ℃ at the rotating speed of
600r/min-1000r/min, stirring for 60min-120min, and naturally cooling to room temperature to obtain the essence.
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| CN103705424B (en) * | 2014-01-02 | 2015-06-10 | 李卿语 | Biological whitening and moisturizing liquid |
| CN104546591A (en) * | 2014-12-12 | 2015-04-29 | 青岛无为保温材料有限公司 | Plant essential oil facial mask and preparation method thereof |
| CN104666178B (en) * | 2015-02-05 | 2017-12-01 | 成都山信药业有限公司 | A kind of skin whitening, moisturizing composition and facial mask |
| CN106236691A (en) * | 2016-09-07 | 2016-12-21 | 高宝化妆品（中国）有限公司 | A kind of antioxidation moisturizing elite dew and preparation method thereof |
| CN106511135B (en) * | 2016-12-22 | 2019-10-11 | 江苏耐雀生物工程技术有限公司 | A kind of whitening compound essential oil and preparation method thereof |
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| CN106994135A (en) * | 2016-01-22 | 2017-08-01 | 德丰铭国际股份有限公司 | It is a kind of that there is the sea grape extract for suppressing allergy function, preparation method and application |
| CN106963717A (en) * | 2017-04-19 | 2017-07-21 | 杨森皓 | A kind of MASQUE APAISANT HYDRATANT |
| CN108158884A (en) * | 2018-02-01 | 2018-06-15 | 百朗德生物化学(海门)有限公司 | With the cosmetic material composition and its application in cosmetics for inhibiting allergy, function of delaying senility |
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