CN110484464A - It is a kind of for preventing and treating the microorganism formulation and organic fertilizer of the withered line disease of rice - Google Patents
It is a kind of for preventing and treating the microorganism formulation and organic fertilizer of the withered line disease of rice Download PDFInfo
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- CN110484464A CN110484464A CN201910700895.2A CN201910700895A CN110484464A CN 110484464 A CN110484464 A CN 110484464A CN 201910700895 A CN201910700895 A CN 201910700895A CN 110484464 A CN110484464 A CN 110484464A
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- 244000005700 microbiome Species 0.000 title claims abstract description 83
- 239000000203 mixture Substances 0.000 title claims abstract description 81
- 238000009472 formulation Methods 0.000 title claims abstract description 78
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 54
- 235000009566 rice Nutrition 0.000 title claims abstract description 54
- 201000010099 disease Diseases 0.000 title claims abstract description 49
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 49
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 15
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000894006 Bacteria Species 0.000 claims abstract description 84
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 76
- 241000209094 Oryza Species 0.000 claims abstract description 53
- 241001460073 Trichoderma asperellum Species 0.000 claims abstract description 39
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 38
- 239000004310 lactic acid Substances 0.000 claims abstract description 38
- 241000751139 Beauveria bassiana Species 0.000 claims abstract description 34
- 241000607715 Serratia marcescens Species 0.000 claims abstract description 30
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 27
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 27
- 239000003337 fertilizer Substances 0.000 claims abstract description 19
- 239000001963 growth medium Substances 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 238000002360 preparation method Methods 0.000 claims description 23
- 239000000470 constituent Substances 0.000 claims description 21
- 238000000855 fermentation Methods 0.000 claims description 21
- 239000000843 powder Substances 0.000 claims description 18
- 241000609240 Ambelania acida Species 0.000 claims description 16
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 16
- 239000001888 Peptone Substances 0.000 claims description 16
- 108010080698 Peptones Proteins 0.000 claims description 16
- 239000010905 bagasse Substances 0.000 claims description 16
- 230000004151 fermentation Effects 0.000 claims description 16
- 239000011159 matrix material Substances 0.000 claims description 16
- 235000019319 peptone Nutrition 0.000 claims description 16
- 235000015278 beef Nutrition 0.000 claims description 15
- 239000008367 deionised water Substances 0.000 claims description 15
- 229910021641 deionized water Inorganic materials 0.000 claims description 15
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 15
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 15
- 230000010355 oscillation Effects 0.000 claims description 15
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 15
- 238000011534 incubation Methods 0.000 claims description 14
- 241000233866 Fungi Species 0.000 claims description 10
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 239000011591 potassium Substances 0.000 claims description 10
- 229910052700 potassium Inorganic materials 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 claims description 8
- 241000255789 Bombyx mori Species 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 240000003183 Manihot esculenta Species 0.000 claims description 8
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 8
- 235000004443 Ricinus communis Nutrition 0.000 claims description 8
- 244000061456 Solanum tuberosum Species 0.000 claims description 8
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- YXVFQADLFFNVDS-UHFFFAOYSA-N diammonium citrate Chemical compound [NH4+].[NH4+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O YXVFQADLFFNVDS-UHFFFAOYSA-N 0.000 claims description 8
- 210000003608 fece Anatomy 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 239000010871 livestock manure Substances 0.000 claims description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 8
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 8
- 229940099596 manganese sulfate Drugs 0.000 claims description 8
- 239000011702 manganese sulphate Substances 0.000 claims description 8
- 235000007079 manganese sulphate Nutrition 0.000 claims description 8
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- 239000002699 waste material Substances 0.000 claims description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 7
- 239000001110 calcium chloride Substances 0.000 claims description 7
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 7
- GNBVPFITFYNRCN-UHFFFAOYSA-M sodium thioglycolate Chemical compound [Na+].[O-]C(=O)CS GNBVPFITFYNRCN-UHFFFAOYSA-M 0.000 claims description 7
- 229940046307 sodium thioglycolate Drugs 0.000 claims description 7
- 229920000136 polysorbate Polymers 0.000 claims description 6
- 230000002265 prevention Effects 0.000 claims description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000011574 phosphorus Substances 0.000 claims description 3
- 229910052698 phosphorus Inorganic materials 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 230000008859 change Effects 0.000 claims description 2
- 239000000460 chlorine Substances 0.000 claims description 2
- 229910052801 chlorine Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 230000000052 comparative effect Effects 0.000 abstract description 23
- 230000000694 effects Effects 0.000 abstract description 6
- 238000012360 testing method Methods 0.000 abstract description 6
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 241000228245 Aspergillus niger Species 0.000 description 10
- 241000607720 Serratia Species 0.000 description 8
- 239000002367 phosphate rock Substances 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 241001530056 Athelia rolfsii Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 241000813090 Rhizoctonia solani Species 0.000 description 2
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 2
- 241000261145 bacterium 3.5 Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000189 neurotoxic Toxicity 0.000 description 1
- 230000002887 neurotoxic effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- VILMUCRZVVVJCA-UHFFFAOYSA-M sodium glycolate Chemical compound [Na+].OCC([O-])=O VILMUCRZVVVJCA-UHFFFAOYSA-M 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 125000000446 sulfanediyl group Chemical group *S* 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of for preventing and treating the microorganism formulation and organic fertilizer of the withered line disease of rice, it is related to technical field of fertilizers, the microorganism formulation contains bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum, lactic acid bacteria, black-koji mould, microorganism formulation of the present invention line disease withered for rice has good control efficiency, using the experimental plot either cave disease incidence or strain disease incidence of microorganism formulation of the present invention below 10%, and it can be seen that by comparative test, bacillus thuringiensis in microorganism formulation of the present invention, serratia marcescens, beauveria bassiana, Trichoderma asperellum, lactic acid bacteria, black-koji mould has good synergistic action effect each other.
Description
Technical field
The present invention relates to technical field of fertilizers, and in particular to a kind of microorganism formulation for preventing and treating the withered line disease of rice and has
Machine fertilizer.
Background technique
Rice is the most important cereal crops of China, but the load class Rhizoctonia solani Kuhn (Rhizoctonia of soil-borne infection
Solani K ü hn) rice sheath blight disease that causes is one of harm rice fungus diseases evil the most serious in global range, especially exists
The high yield and stable yields of rice are seriously hindered in intensive cropping system.In recent years as the reinforcing of Rice Production system, plantation are close
The raising of degree and the utilization of high-yield rice kind, banded sclerotial blight are on the rise in the harm of China.When natural conditions are conducive to cause of disease
Bacterium grow when, caused by yield damage may be up to 50%.In addition, the rice sheath blight disease epidemic-stricken area in China is up to 1500 to 2000
Ten thousand hm2, about 6,000,000 t of the production loss having resulted in.The disease because host is extensive, sclerotium long-term surviving in two season crop soil
In, and the characteristic strong with hereditary variability, it makes it difficult to control.
Now, the major measure for preventing and treating rice sheath blight disease has agricultural, chemistry and biological control.Wherein, salvage sclerotium, rationally
Cultivation management and breeding resistant variety are the important means of cultural control, but its effect is limited, and resistant gene is single, transgenosis
Safety and it is difficult to improve the outstanding problems urgent need to resolve such as the lasting validity of resistant variety.And chemical prevention has economy due to it
It efficiently, is always the indispensable part of Synthetical prevention rice sheath blight disease the advantages that wide sterilization spectrum, prevention and treatment simplicity.But excessively
Cause numerous following problems using chemical pesticide, such as: environmental pollution, human health is worsening, banded sclerotial blight is resistance to
Pharmacological property enhancing, or even other beneficial organisms are also compromised when murdering sheath blight fungus, rice field ecology balance is destroyed, is led indirectly
Cause the rice underproduction.Biological control is acknowledged as preventing and treating during modern agricultural production because of good environmental compatible and sustainability
The important channel of rice sheath blight disease, but currently used for prevention and treatment rice sheath blight disease microorganism formulation effect be not it is very good, also need
Want further Improvement.
Summary of the invention
It is an object of the present invention to the microorganism formulation that the present invention provides a kind of for preventing and treating the withered line disease of rice and organic
Fertilizer.
In order to achieve the above object, the present invention is achieved by the following technical programs:
It is a kind of for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the microorganism formulation contains Su Yunjin bud
Spore bacillus (Bacillius thuringiensis, Bt), serratia marcescens (Serratia marcesces), ball spore
Muscardine (Beauveria bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria
(lactic acid bacteria, LAB), black-koji mould (Aspergillus niger).
Further, the microorganism formulation is liquid microbe preparation or powder microorganism formulation.
Further, bacillus thuringiensis 3-6 × 10 in the liquid microbe preparation12Cfu/mL, Serratia
Bacterium 1.2-1.5 × 1012Cfu/mL, beauveria bassiana 2.2-3 × 1012Cfu/mL, Trichoderma asperellum 3-6 × 1011Cfu/mL, cream
Sour bacterium 5-8 × 1010Cfu/mL, black-koji mould 0.1-0.4 × 108cfu/mL。
Further, bacillus thuringiensis 2.3-5 × 10 in the powder microorganism formulation12Cfu/mL, cement sand thunder
Salmonella 1.03-1.47 × 1012Cfu/mL, beauveria bassiana 3.5-4 × 1012Cfu/mL, Trichoderma asperellum 1-2 × 1011cfu/mL
, lactic acid bacteria 0.22-0.48 × 1011Cfu/mL, black-koji mould 2-2.6 × 108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
(1) bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into first by a certain amount of
It is mixed in culture medium, ferment 2.5-5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 25-28 DEG C of temperature,
Revolving speed 100-200rpm, shimmy amplitude 10-15mm, air intake 300-500L/h；
(2) lactic acid bacteria, black-koji mould are mixed by a certain amount of be inoculated into the second culture medium, is detested in constant temperature incubation oscillation shaking table
Aerobe fermentation 2.5-5d obtains bacterium solution B, fermentation parameter are as follows: and 25-28 DEG C of temperature, revolving speed 50-80rpm, shimmy amplitude 5-12mm；
(3) it is diluted with water or is concentrated after mixing bacterium solution A with bacterium solution B, dry the microorganism formulation can be obtained.
Further, the first culture medium includes the constituent of following weight fraction:
4-8 parts of beef extract, 20-30 parts of potato, 10-15 parts of peptone, 2-3 parts of sucrose, 0.2-0.4 parts of L-cysteine, chlorine
Change sodium 3-5 parts, 0.1-0.4 parts of potassium dihydrogen phosphate, 1000-1200 parts of deionized water.
Further, the second culture medium includes the constituent of following weight fraction:
5-10 parts of beef extract, 24-28 parts of peptone, 1-3 parts of bean cake powder, 0.1-0.5 parts of potassium dihydrogen phosphate, bagasse 0.5-0.7
Part, 1-1.4 parts of glucose, 1.1-1.4 parts of tween, 0.03-0.07 parts of dibasic ammonium citrate, 0.3-0.5 parts of magnesium sulfate, manganese sulfate
0.6-1.5 parts, 2.5-4 parts of sodium thioglycolate, 1000-1200 parts of deionized water.
Application of the mentioned microorganism preparation in the withered line disease of prevention and treatment rice.
The present invention also provides a kind of organic fertilizer including mentioned microorganism preparation, the ingredient of organic fertilizer includes matrix fertilizer
Material, microorganism formulation and EM bacteria agent, three's weight ratio are 800-1000:1:0.1-0.3；
Matrix fertilizer includes the constituent of following weight fraction:
10-16 parts of barnyard manure, 8-12 parts of castor cake, 1-4 parts of silkworm excrement, 5-7 parts of vinasse, 3-5 parts of bagasse, 1-2 parts of potassium breeze, phosphorus ore
0.5-1 parts of powder, 1-1.5 parts of calcium chloride, 6-12 parts of edible fungi residues, 5-10 parts of manioc waste.
The invention has the following advantages:
Bacillus thuringiensis can produce two major classes toxin, i.e. endotoxin (parasporal crystal) and outer poison in microorganism formulation of the present invention
Element, after pests, under the effect of enteron aisle alkaline digest liquid, thallus discharges toxin, and pest is poisoned and stops feeding, last pest
Because hungry and blood and neurotoxic are dead, and the system of defense of rice can be effectively activated, raising resists the withered line disease of rice
Property；
Serratia marcescens disease can compete identical nutriment in rice leaf and root system with Rhizoctonia solani Kuhn, make its due to
Lack nutriment and dead；
Beauveria bassiana is broad spectrum insecticide fungi, its own can exactly efficiently control population number, while not injure other
Natural enemy insect and beneficial organism；
Trichoderma asperellum can not only secrete organic acid, and that the minerals that plant cannot directly absorb are degraded into phosphorous, potassium etc. is organic
Fertilizer, and the growth that the auxins such as gibberellin, heteroauxin directly facilitate plant can be secreted, it furthermore can also improve plant
Microbiologic population in object root soil promotes the growth of plant indirectly；
Lactic acid bacteria, black-koji mould can be played as collaboration strain further to be promoted to improve rice tillering number, spike number, dry granular quality
With Soil Microorganism to the Utilization ability of nutriment；
Microorganism formulation of the present invention line disease withered for rice has good control efficiency, uses the test of microorganism formulation of the present invention
Field either cave disease incidence or strain disease incidence are 10% hereinafter, and can be seen that microorganism of the present invention by comparative test
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum, lactic acid bacteria, black-koji mould are mutual in preparation
Between have good synergistic action effect.
Detailed description of the invention
Fig. 1 is the flow chart of microorganism formulation preparation method of the present invention.
Specific embodiment
Embodiment 1:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contains bacillus thuringiensis (Bacillius
Thuringiensis, Bt), serratia marcescens (Serratia marcesces), beauveria bassiana (Beauveria
Bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria (lactic acid bacteria,
LAB), black-koji mould (Aspergillus niger).
The microorganism formulation is liquid microbe preparation；
Wherein, bacillus thuringiensis 3.6 × 1012Cfu/mL, serratia marcescens 1.4 × 1012Cfu/mL, beauveria bassiana
2.5×1012Cfu/mL, Trichoderma asperellum 5 × 1011Cfu/mL, lactic acid bacteria 6.6 × 1010Cfu/mL, black-koji mould 0.13 ×
108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into the first culture by a certain amount of
It is mixed in base, ferment 2.5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 26 DEG C of temperature, revolving speed
150rpm, shimmy amplitude 12mm, air intake 350L/h；First culture medium includes the constituent of following weight fraction: ox
5 parts of meat extract, 22 parts of potato, 14 parts of peptone, 2.2 parts of sucrose, 0.23 part of L-cysteine, 4 parts of sodium chloride, potassium dihydrogen phosphate
0.15 part, 1200 parts of deionized water are mixed lactic acid bacteria, black-koji mould by a certain amount of be inoculated into the second culture medium, Yu Hengwen
Anaerobic fermentation 2.5d in culture oscillation shaking table, obtains bacterium solution B, fermentation parameter are as follows: and 26 DEG C of temperature, revolving speed 80rpm, shimmy amplitude
10mm；Second culture medium includes the constituent of following weight fraction: 8 parts of beef extract, 25 parts of peptone, 1.5 parts of bean cake powder, phosphorus
0.3 part of acid dihydride potassium, 0.55 part of bagasse, 1.2 parts of glucose, 1.2 parts of tween, 0.06 part of dibasic ammonium citrate, magnesium sulfate 0.3
Part, 1 part of manganese sulfate, 3 parts of sodium thioglycolate, 1200 parts of deionized water, are diluted with water or dense after bacterium solution A is mixed with bacterium solution B
The microorganism formulation can be obtained in contracting, drying.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 1000:1:0.2；
Matrix fertilizer includes the constituent of following weight fraction:
14 parts of barnyard manure, 8 parts of castor cake, 3 parts of silkworm excrement, 6 parts of vinasse, 4 parts of bagasse, 1 part of potassium breeze, 0.6 part of ground phosphate rock, calcium chloride
1.5 parts, 10 parts of edible fungi residues, 8 parts of manioc waste.
Embodiment 2:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contains bacillus thuringiensis (Bacillius
Thuringiensis, Bt), serratia marcescens (Serratia marcesces), beauveria bassiana (Beauveria
Bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria (lactic acid bacteria,
LAB), black-koji mould (Aspergillus niger)；
The microorganism formulation is liquid microbe preparation；
Wherein, bacillus thuringiensis 3 × 1012Cfu/mL, serratia marcescens 1.5 × 1012Cfu/mL, beauveria bassiana 2.3
×1012Cfu/mL, Trichoderma asperellum 4 × 1011Cfu/mL, lactic acid bacteria 5.5 × 1010Cfu/mL, black-koji mould 0.33 ×
108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into the first culture by a certain amount of
It is mixed in base, ferment 5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 28 DEG C of temperature, revolving speed 120rpm,
Shimmy amplitude 15mm, air intake 500L/h；First culture medium includes the constituent of following weight fraction: 8 parts of beef extract,
25 parts of potato, 15 parts of peptone, 3 parts of sucrose, 0.3 part of L-cysteine, 5 parts of sodium chloride, 0.1 part of potassium dihydrogen phosphate, go from
1000 parts of sub- water is mixed lactic acid bacteria, black-koji mould by a certain amount of be inoculated into the second culture medium, vibrates shaking table in constant temperature incubation
Middle anaerobic fermentation 5d, obtains bacterium solution B, fermentation parameter are as follows: and 28 DEG C of temperature, revolving speed 60rpm, shimmy amplitude 10mm；Second culture medium
Constituent including following weight fraction: 10 parts of beef extract, 25 parts of peptone, 3 parts of bean cake powder, 0.5 part of potassium dihydrogen phosphate, sweet
It is 0.6 part of bagasse, 1.4 parts of glucose, 1.2 parts of tween, 0.03 part of dibasic ammonium citrate, 0.5 part of magnesium sulfate, 0.6 part of manganese sulfate, thio
4 parts of sodium glycollate, 1000 parts of deionized water are diluted with water or are concentrated after mixing bacterium solution A with bacterium solution B, dry institute can be obtained
State microorganism formulation.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 1000:1:0.1；
Matrix fertilizer includes the constituent of following weight fraction:
10 parts of barnyard manure, 12 parts of castor cake, 3 parts of silkworm excrement, 6 parts of vinasse, 4 parts of bagasse, 1 part of potassium breeze, 1 part of ground phosphate rock, calcium chloride
1.3 parts, 9 parts of edible fungi residues, 8 parts of manioc waste.
Embodiment 3:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contains bacillus thuringiensis (Bacillius
Thuringiensis, Bt), serratia marcescens (Serratia marcesces), beauveria bassiana (Beauveria
Bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria (lactic acid bacteria,
LAB), black-koji mould (Aspergillus niger).
The microorganism formulation is powder microorganism formulation.
Wherein, bacillus thuringiensis 5 × 1012Cfu/mL, serratia marcescens 1.36 × 1012Cfu/mL, the white deadlock of ball spore
Bacterium 3.5 × 1012Cfu/mL, Trichoderma asperellum 1.1 × 1011Cfu/mL, lactic acid bacteria 0.4 × 1011Cfu/mL, black-koji mould 2.5
×108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into the first culture by a certain amount of
It is mixed in base, ferment 5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 25 DEG C of temperature, revolving speed 200rpm,
Shimmy amplitude 12mm, air intake 400L/h；
First culture medium includes the constituent of following weight fraction: 5 parts of beef extract, 20 parts of potato, 15 parts of peptone, sucrose
2 parts, 0.4 part of L-cysteine, 4 parts of sodium chloride, 0.1 part of potassium dihydrogen phosphate, 1200 parts of deionized water, by lactic acid bacteria, aspergillus niger
Bacterium is mixed by a certain amount of be inoculated into the second culture medium, and the anaerobic fermentation 5d in constant temperature incubation oscillation shaking table obtains bacterium solution B, sends out
Ferment parameter are as follows: 28 DEG C of temperature, revolving speed 60rpm, shimmy amplitude 10mm；Second culture medium include following weight fraction composition at
Point: 8 parts of beef extract, 3 parts of bean cake powder, 0.5 part of potassium dihydrogen phosphate, 0.5 part of bagasse, 1.4 parts of glucose, is spat 25 parts of peptone
Warm 1.1 parts, 0.05 part of dibasic ammonium citrate, 0.5 part of magnesium sulfate, 1 part of manganese sulfate, 3 parts of sodium thioglycolate, deionized water 1000
Part, it is diluted with water or is concentrated after bacterium solution A is mixed with bacterium solution B, dry the microorganism formulation can be obtained.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 800:1:0.2；
Matrix fertilizer includes the constituent of following weight fraction:
12 parts of barnyard manure, 12 parts of castor cake, 2 parts of silkworm excrement, 6 parts of vinasse, 3 parts of bagasse, 1 part of potassium breeze, 0.6 part of ground phosphate rock, chlorination
1.2 parts of calcium, 10 parts of edible fungi residues, 8 parts of manioc waste.
Embodiment 4:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contains bacillus thuringiensis (Bacillius
Thuringiensis, Bt), serratia marcescens (Serratia marcesces), beauveria bassiana (Beauveria
Bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria (lactic acid bacteria,
LAB), black-koji mould (Aspergillus niger).
The microorganism formulation is powder microorganism formulation.
Wherein bacillus thuringiensis 2.3 × 1012Cfu/mL, serratia marcescens 1.03 × 1012Cfu/mL, ball spore are white
Stiff bacterium 3.5 × 1012Cfu/mL, Trichoderma asperellum 1 × 1011Cfu/mL, lactic acid bacteria 0.22 × 1011Cfu/mL, black-koji mould 2 ×
108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into the first culture by a certain amount of
It is mixed in base, ferment 2.5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 25 DEG C of temperature, revolving speed
100rpm, shimmy amplitude 10mm, air intake 300L/h；
First culture medium includes the constituent of following weight fraction: 4 parts of beef extract, 20 parts of potato, 10 parts of peptone, sucrose
2 parts, 0.2 part of L-cysteine, 3 parts of sodium chloride, 0.1 part of potassium dihydrogen phosphate, 1000 parts of deionized water, by lactic acid bacteria, aspergillus niger
Bacterium is mixed by a certain amount of be inoculated into the second culture medium, and the anaerobic fermentation 2.5d in constant temperature incubation oscillation shaking table obtains bacterium solution B,
Fermentation parameter are as follows: 25 DEG C of temperature, revolving speed 50rpm, shimmy amplitude 5mm；Second culture medium include following weight fraction composition at
Point: 5 parts of beef extract, 24 parts of peptone, 1 part of bean cake powder, 0.1 part of potassium dihydrogen phosphate, 0.5 part of bagasse, 1 part of glucose, tween
1.1 parts, 0.03 part of dibasic ammonium citrate, 0.3 part of magnesium sulfate, 0.6 part of manganese sulfate, 2.5 parts of sodium thioglycolate, deionized water 1000
Part, it is diluted with water or is concentrated after bacterium solution A is mixed with bacterium solution B, dry the microorganism formulation can be obtained.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 800:1:0.1；
Matrix fertilizer includes the constituent of following weight fraction:
10 parts of barnyard manure, 8 parts of castor cake, 1 part of silkworm excrement, 5 parts of vinasse, 3 parts of bagasse, 1 part of potassium breeze, 0.5 part of ground phosphate rock, calcium chloride
1 part, 6 parts of edible fungi residues, 5 parts of manioc waste.
Embodiment 5:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contains bacillus thuringiensis (Bacillius
Thuringiensis, Bt), serratia marcescens (Serratia marcesces), beauveria bassiana (Beauveria
Bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria (lactic acid bacteria,
LAB), black-koji mould (Aspergillus niger).
The microorganism formulation is liquid microbe preparation.
Wherein bacillus thuringiensis 6 × 1012Cfu/mL, serratia marcescens 1.5 × 1012Cfu/mL, beauveria bassiana
3×1012Cfu/mL, Trichoderma asperellum 6 × 1011Cfu/mL, lactic acid bacteria 8 × 1010Cfu/mL, black-koji mould 0.4 × 108cfu/
mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into the first culture by a certain amount of
It is mixed in base, ferment 5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 28 DEG C of temperature, revolving speed 200rpm,
Shimmy amplitude 15mm, air intake 500L/h；
First culture medium includes the constituent of following weight fraction: 8 parts of beef extract, 30 parts of potato, 15 parts of peptone, sucrose
3 parts, 0.4 part of L-cysteine, 5 parts of sodium chloride, 0.4 part of potassium dihydrogen phosphate, 1200 parts of deionized water, by lactic acid bacteria, aspergillus niger
Bacterium is mixed by a certain amount of be inoculated into the second culture medium, and the anaerobic fermentation 5d in constant temperature incubation oscillation shaking table obtains bacterium solution B, sends out
Ferment parameter are as follows: 28 DEG C of temperature, revolving speed 80rpm, shimmy amplitude 12mm；Second culture medium include following weight fraction composition at
Point: 10 parts of beef extract, 3 parts of bean cake powder, 0.5 part of potassium dihydrogen phosphate, 0.7 part of bagasse, 1.4 parts of glucose, is spat 28 parts of peptone
Warm 1.4 parts, 0.07 part of dibasic ammonium citrate, 0.5 part of magnesium sulfate, 1.5 parts of manganese sulfate, 4 parts of sodium thioglycolate, deionized water 1200
Part, it is diluted with water or is concentrated after bacterium solution A is mixed with bacterium solution B, dry the microorganism formulation can be obtained.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 1000:1:0.3；
Matrix fertilizer includes the constituent of following weight fraction:
16 parts of barnyard manure, 12 parts of castor cake, 4 parts of silkworm excrement, 7 parts of vinasse, 5 parts of bagasse, 2 parts of potassium breeze, 1 part of ground phosphate rock, calcium chloride
1.5 parts, 12 parts of edible fungi residues, 10 parts of manioc waste.
Comparative example 1:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contain serratia marcescens (Serratia marcesces),
Beauveria bassiana (Beauveria bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria
(lactic acid bacteria, LAB), black-koji mould (Aspergillus niger).
The microorganism formulation is liquid microbe preparation.
Wherein, serratia marcescens 1.4 × 1012Cfu/mL, beauveria bassiana 2.5 × 1012Cfu/mL, Trichoderma asperellum 5
×1011Cfu/mL, lactic acid bacteria 6.6 × 1010Cfu/mL, black-koji mould 0.13 × 108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Serratia marcescens, beauveria bassiana, Trichoderma asperellum are mixed by a certain amount of be inoculated into the first culture medium, Yu Hengwen
Ferment 2.5d in culture oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: and 26 DEG C of temperature, revolving speed 150rpm, shimmy amplitude 12mm,
Air intake 350L/h；First culture medium includes the constituent of following weight fraction: 5 parts of beef extract, 22 parts of potato, egg
14 parts of white peptone, 2.2 parts of sucrose, 0.23 part of L-cysteine, 4 parts of sodium chloride, 0.15 part of potassium dihydrogen phosphate, deionized water 1200
Part, lactic acid bacteria, black-koji mould are mixed by a certain amount of be inoculated into the second culture medium, the anaerobism hair in constant temperature incubation oscillation shaking table
Ferment 2.5d obtains bacterium solution B, fermentation parameter are as follows: and 26 DEG C of temperature, revolving speed 80rpm, shimmy amplitude 10mm；Second culture medium include with
The constituent of lower weight fraction:
8 parts of beef extract, 25 parts of peptone, 1.5 parts of bean cake powder, 0.3 part of potassium dihydrogen phosphate, 0.55 part of bagasse, glucose 1.2
Part, 1.2 parts of tween, 0.06 part of dibasic ammonium citrate, 0.3 part of magnesium sulfate, 1 part of manganese sulfate, 3 parts of sodium thioglycolate, deionized water
It 1200 parts, is diluted with water or is concentrated after bacterium solution A is mixed with bacterium solution B, dry the microorganism formulation can be obtained.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 1000:1:0.2；
Matrix fertilizer includes the constituent of following weight fraction:
14 parts of barnyard manure, 8 parts of castor cake, 3 parts of silkworm excrement, 6 parts of vinasse, 4 parts of bagasse, 1 part of potassium breeze, 0.6 part of ground phosphate rock, calcium chloride
1.5 parts, 10 parts of edible fungi residues, 8 parts of manioc waste.
Comparative example 1 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain bacillus thuringiensis.
Comparative example 2:
Comparative example 2 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain serratia marcescens.
Comparative example 3:
Comparative example 3 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain beauveria bassiana.
Comparative example 4:
Comparative example 4 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain Trichoderma asperellum.
Comparative example 5:
Comparative example 5 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain lactic acid bacteria.
Comparative example 6:
Comparative example 6 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain black-koji mould.
Planting experiment:
The microorganism formulation for being utilized respectively 1-5 of the embodiment of the present invention and comparative example 1-6 handles rice seedling；
Wherein embodiment 1,2,5, comparative example 1-6 are liquid microbe preparation, are diluted to after 1000 times to rice seedling
Carry out root dipping processing；
Embodiment 3,4 is powder microorganism formulation, again by powder microorganism formulation after rice seedling root steam is moistened by spraying
Sprinkling is adhered to its root；
The rice seedling of blank example is not processed.
It is " southern round-grained rice 9108 " for examination rice varieties.Test is in the town the He Feng village Sun Jiaxiang, Nanjing Lishui area.Investigation records
The cave of different disposal and strain disease incidence, disease index, banded sclerotial blight investigation method are carried out according to GB/T17980.20-2000.
Test result is as shown in table 1 below:
Table 1:
By upper table 1 it is found that microorganism formulation of the present invention line disease withered for rice has good control efficiency, the micro- life of the present invention is used
The experimental plot of object preparation either cave disease incidence or strain disease incidence by comparative test 10% hereinafter, and can be seen that
Bacillus thuringiensis in microorganism formulation of the present invention, serratia marcescens, beauveria bassiana, Trichoderma asperellum, lactic acid bacteria,
Black-koji mould has good synergistic action effect each other.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features；And these modification or
Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (10)
1. a kind of for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the microorganism formulation contains Su Yunjin
Bacillus, serratia marcescens, beauveria bassiana, Trichoderma asperellum, lactic acid bacteria, black-koji mould.
2. as described in claim 1 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the microorganism system
Agent is liquid microbe preparation or powder microorganism formulation.
3. as described in claim 1 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the micro- life of liquid
Bacillus thuringiensis 3-6 × 10 in object preparation12Cfu/mL, serratia marcescens 1.2-1.5 × 1012Cfu/mL, the white deadlock of ball spore
Bacterium 2.2-3 × 1012Cfu/mL, Trichoderma asperellum 3-6 × 1011Cfu/mL, lactic acid bacteria 5-8 × 1010Cfu/mL, black-koji mould
0.1-0.4×108cfu/mL。
4. as described in claim 1 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the micro- life of powder
Bacillus thuringiensis 2.3-5 × 10 in object preparation12Cfu/mL, serratia marcescens 1.03-1.47 × 1012Cfu/mL, ball spore
Muscardine 3.5-4 × 1012Cfu/mL, Trichoderma asperellum 1-2 × 1011Cfu/mL, lactic acid bacteria 0.22-0.48 × 1011cfu/mL、
Black-koji mould 2-2.6 × 108cfu/mL。
5. as described in claim 1 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that preparation method is such as
Under:
(1) bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into first by a certain amount of
It is mixed in culture medium, ferment 2.5-5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 25-28 DEG C of temperature,
Revolving speed 100-200rpm, shimmy amplitude 10-15mm, air intake 300-500L/h；
(2) lactic acid bacteria, black-koji mould are mixed by a certain amount of be inoculated into the second culture medium, is detested in constant temperature incubation oscillation shaking table
Aerobe fermentation 2.5-5d obtains bacterium solution B, fermentation parameter are as follows: and 25-28 DEG C of temperature, revolving speed 50-80rpm, shimmy amplitude 5-12mm；
(3) it is diluted with water or is concentrated after mixing bacterium solution A with bacterium solution B, dry the microorganism formulation can be obtained.
6. as claimed in claim 5 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the first culture medium packet
Include the constituent of following weight fraction:
4-8 parts of beef extract, 20-30 parts of potato, 10-15 parts of peptone, 2-3 parts of sucrose, 0.2-0.4 parts of L-cysteine, chlorine
Change sodium 3-5 parts, 0.1-0.4 parts of potassium dihydrogen phosphate, 1000-1200 parts of deionized water.
7. as claimed in claim 5 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the second culture medium packet
Include the constituent of following weight fraction:
5-10 parts of beef extract, 24-28 parts of peptone, 1-3 parts of bean cake powder, 0.1-0.5 parts of potassium dihydrogen phosphate, bagasse 0.5-0.7
Part, 1-1.4 parts of glucose, 1.1-1.4 parts of tween, 0.03-0.07 parts of dibasic ammonium citrate, 0.3-0.5 parts of magnesium sulfate, manganese sulfate
0.6-1.5 parts, 2.5-4 parts of sodium thioglycolate, 1000-1200 parts of deionized water.
8. such as application of the microorganism formulation of any of claims 1-7 in the withered line disease of prevention and treatment rice.
9. a kind of organic fertilizer, which is characterized in that including microorganism formulation such as of any of claims 1-7.
10. organic fertilizer as claimed in claim 9, which is characterized in that including matrix fertilizer, microorganism formulation and EM bacteria agent, three
Person's weight ratio is 800-1000:1:0.1-0.3；
Described matrix fertilizer includes the constituent of following weight fraction:
10-16 parts of barnyard manure, 8-12 parts of castor cake, 1-4 parts of silkworm excrement, 5-7 parts of vinasse, 3-5 parts of bagasse, 1-2 parts of potassium breeze, phosphorus ore
0.5-1 parts of powder, 1-1.5 parts of calcium chloride, 6-12 parts of edible fungi residues, 5-10 parts of manioc waste.
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| CN115490557A (en) * | 2022-10-27 | 2022-12-20 | 中昇南阳生物科技有限公司 | Compound microbial fertilizer for preventing and treating rice sheath blight disease and preparation method thereof |
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Denomination of invention: A microbial preparation and organic fertilizer for the prevention and control of rice stripe disease Granted publication date: 20210611 Pledgee: Bank of China Limited Nanjing Chengdong Branch Pledgor: Nanjing ningliang Environmental Protection Group Co.,Ltd. Registration number: Y2024980032554 |
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