CN110237877B - Microfluidic device and droplet control method - Google Patents
Microfluidic device and droplet control method Download PDFInfo
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- CN110237877B CN110237877B CN201910569444.XA CN201910569444A CN110237877B CN 110237877 B CN110237877 B CN 110237877B CN 201910569444 A CN201910569444 A CN 201910569444A CN 110237877 B CN110237877 B CN 110237877B
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- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
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Abstract
The application provides a microfluidic device and a droplet control method, and relates to the field of microfluidics. The microfluidic device comprises: the device comprises a thermosensitive induction layer, a heating layer, a driving assembly and a control module; a flow channel for containing liquid drops is arranged between the heating layer and the thermosensitive induction layer; the heat-sensitive induction layer is used for inducing the heat transferred by the heating layer and converting the heat into a heat electric signal to be output; and the control module is used for receiving the thermal electric signal, determining the current position of the liquid drop and controlling the driving assembly to drive the liquid drop to move to the preset position. The control method comprises the following steps: the heating layer heats uniformly; converting the heat induced by the thermosensitive induction layer into a thermal electric signal; determining the current position of the liquid drop according to the difference of the thermal electric signals at the liquid drop position and the non-liquid drop position; and controlling a driving assembly to drive the liquid drop to move to a preset position according to the current position. The microfluidic device is simple in structure, low in manufacturing cost and simple in process method, and can realize efficient and accurate liquid drop control.
Description
Technical Field
The invention relates to the field of microfluidics, in particular to a microfluidic device and a liquid drop control method.
Background
Micro-fluidic (Micro-fluidic) technology is a technology that is mainly characterized by manipulation of fluids in the Micro-scale space. The technology is crossed with chemical, biological, engineering, physics and other subjects, and shows wide application prospect.
The early concept of microfluidics can be traced back to gas chromatographs fabricated on silicon wafers by photolithography in the century, and then developed into microfluidic capillary electrophoresis instruments, microreactors and the like. One of the important features of microfluidics is the unique fluid properties in microscale environments, such as laminar flow and droplets. With these unique fluidic phenomena, microfluidics can achieve a range of microfabrication and micromanipulation that are difficult to accomplish with conventional methods.
Microfluidics is currently considered to have great development potential and broad application prospects in biomedical research.
However, the existing microfluidic devices have the following disadvantages: the structure is more complicated, and the cost of manufacture is higher to the precision of liquid drop control still needs to be promoted.
Disclosure of Invention
The invention aims to provide a microfluidic device and a control method, the microfluidic device has a simple structure and low manufacturing cost, and can realize efficient and accurate droplet control.
To achieve the above object, in a first aspect, the present application provides a microfluidic device comprising: the device comprises a thermosensitive induction layer, a heating layer for uniformly heating, a driving component for driving liquid drops to move and a control module;
a flow channel for containing liquid drops is arranged between the heating layer and the thermosensitive induction layer;
the heat-sensitive induction layer is used for inducing heat transferred by the heating layer and converting the heat into a heat electric signal to be output;
the control module is electrically connected with the thermosensitive sensing layer and used for receiving the thermal electric signals, determining the current position of the liquid drop according to the difference of the thermal electric signals at the liquid drop position and the non-liquid drop position, and controlling the driving assembly to drive the liquid drop to move to the preset position according to the current position.
In an alternative embodiment, the drive assembly comprises a first electrode layer and a second electrode layer arranged in parallel;
the second electrode layer is closer to the heat-sensitive sensing layer than the first electrode layer;
an electric field for driving the liquid drop to move can be formed between the first electrode layer and the second electrode layer.
In an alternative embodiment, the first electrode layer comprises a plurality of uniformly arranged first electrodes and the second electrode layer comprises a plurality of uniformly arranged second electrodes;
the projections of the plurality of first electrodes and the plurality of second electrodes in the same plane are vertical;
the intersections of the plurality of first electrodes and the plurality of second electrodes form a plurality of uniformly distributed control bits on the second electrode layer;
the control module is used for adjusting the voltage applied to the first electrode and the second electrode, so that a voltage difference is formed between adjacent control bits, and the liquid drop is driven to move from the control bit at the current position to the next adjacent control bit until the liquid drop moves to a preset position.
In an alternative embodiment, the first electrode and the second electrode are both rectangular and long, and the widths of the first electrode and the second electrode are the same;
the control position is square, and the diameter of the liquid drop is not less than the side length of the control position.
In an alternative embodiment, the thermally sensitive sensing layer comprises a plurality of sensors;
each sensor is used for storing position information corresponding to the sensor, converting the heat into a voltage or current signal and sending the voltage or current signal and the position information to the control module;
the control module is electrically connected with each sensor, and is specifically used for comparing voltage or current signals of each sensor, determining the sensor corresponding to the position of the liquid drop, and determining the position information of the liquid drop according to the position information of the sensor.
In an alternative embodiment, the positions of a plurality of the sensors correspond to the positions of a plurality of the control bits one to one.
In an optional embodiment, the heat generating layer is disposed between the first electrode layer and the second electrode layer, and the flow channel is located between the second electrode layer and the heat sensitive layer.
In an optional embodiment, the top surface of the second electrode layer is provided with a first insulating layer, and the bottom surface of the heat-sensitive sensing layer is provided with a second insulating layer;
the runner is located between the first insulating layer and the second insulating layer.
In an optional embodiment, the opposite surfaces of the first insulating layer and the second insulating layer are provided with hydrophobic layers; or the like, or, alternatively,
the material of the first insulating layer and the material of the second insulating layer are both hydrophobic materials.
In a second aspect, the present application further provides a droplet control method, comprising the steps of:
when the liquid drops are positioned between the heating layer and the thermosensitive induction layer, the heating layer is controlled to uniformly heat;
receiving a heat electric signal output by the thermosensitive sensing layer; the heat electric signal is obtained by converting the heat sensitive sensing layer according to the sensed heat;
determining the current position of the liquid drop according to the difference of the thermal electric signals at the liquid drop and the non-liquid drop;
and controlling a driving assembly to drive the liquid drop to move to a preset position according to the current position.
In an alternative embodiment, said determining the current position of the droplet from the difference in the electrical heat signal at the droplet and at the non-droplet comprises:
comparing the received voltage or current signals to determine at least one voltage or current signal that is different from the other voltage or current signals;
and according to the determined at least one voltage or current signal, determining position information corresponding to the voltage or current signal to obtain the current position of the liquid drop.
Compared with the prior art, the scheme of the invention has the following advantages:
the utility model provides a layer that generates heat evenly generates heat, upwards shine from the bottom heat source of liquid drop, because liquid medium heat conduction rate has the difference with air medium for the thermosensitive induction layer of liquid drop department and non-liquid drop department is heated differently, and the heat that senses is different, thereby there is the difference in the heat signal of heat that the heat of liquid drop department and non-liquid drop department turned into, and control module confirms the current position of liquid drop according to the heat signal of electricity, again according to current position control drive assembly drive liquid drop remove to preset position.
The micro-fluidic device adopts the heating layer, the thermosensitive induction layer, the driving assembly and the control module, can realize the positioning and the movement of liquid drops, and has the advantages of simple integral structure and lower manufacturing cost.
The application provides a new control idea, and the method has the advantages that the process method is simple and efficient and accurate liquid drop control can be realized through the heat induction mode of the thermosensitive induction layer.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The foregoing and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
fig. 1 is a schematic structural diagram of a microfluidic device according to an embodiment of the present invention.
FIG. 2 is a flow chart of a droplet control method according to an embodiment of the invention.
Reference numerals: 1-first electrode layer, 2-heat generating layer, 3-second electrode layer, 4-first insulating layer, 5-second insulating layer, 6-thermosensitive sensing layer, 7-liquid drop.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative only and should not be construed as limiting the invention.
Referring to fig. 1, an embodiment of the present application provides a microfluidic device including: the device comprises a thermosensitive induction layer 6, a heating layer 2 for uniformly heating, a driving component for driving liquid drops 7 to move and a control module;
a flow channel for containing liquid drops 7 is arranged between the heating layer 2 and the heat-sensitive induction layer 6;
the heat-sensitive induction layer 6 is used for inducing the heat transferred by the heating layer 2 and converting the heat into a heat electric signal to be output;
and the control module is electrically connected with the thermosensitive sensing layer 6 and used for receiving the thermal electric signals, determining the current position of the liquid drop 7 according to the difference of the thermal electric signals at the liquid drop 7 and the non-liquid drop 7, and controlling the driving assembly to drive the liquid drop 7 to move to the preset position according to the current position. Wherein, the control module also can be connected with layer 2 electricity that generates heat, and the layer 2 that controls generates heat evenly generates heat.
The working principle of the micro-fluidic device based on the embodiment of the application is as follows: the heating layer 2 heats uniformly to ensure that the channels of the whole micro-fluidic device and the heat-sensitive induction layer 6 are heated uniformly from the bottom. The heating layer 2 heats and irradiates upwards from a bottom heat source of the liquid drops 7, due to the fact that the heat conduction rate of the liquid medium is different from that of the air medium, the heat sensitive sensing layers at the positions of the liquid drops 7 and the positions of the non-liquid drops 7 are heated differently, the sensed heat is different, and therefore the heat at the positions of the liquid drops 7 and the positions of the non-liquid drops 7 are converted into heat electric signals which are different, the control module determines the current position of the liquid drops 7 according to the heat electric signals, and then the driving assembly is controlled to drive the liquid drops 7 to move to the preset position according to the current position.
The micro-fluidic device adopts the heating layer 2, the thermosensitive induction layer 6, the driving component and the control module, can realize the positioning and movement of the liquid drop 7, and has simple integral structure and lower manufacturing cost; the position of the liquid drop is determined in a heat sensing mode of the heat sensing layer 6, the process method is simple, and efficient and accurate control of the liquid drop 7 can be achieved.
Alternatively, as a driving structure of the driving assembly, the driving assembly includes a first electrode layer 1 and a second electrode layer 3 disposed in parallel; the second electrode layer 3 is closer to the heat-sensitive sensing layer 6 than the first electrode layer 1; an electric field for driving the movement of the liquid droplets 7 may be formed between the first electrode layer 1 and the second electrode layer 3.
Alternatively, the first electrode layer 1 comprises a plurality of uniformly arranged first electrodes, and the second electrode layer 3 comprises a plurality of uniformly arranged second electrodes;
the projections of the plurality of first electrodes and the plurality of second electrodes in the same plane are vertical;
the intersections of the plurality of first electrodes and the plurality of second electrodes form a plurality of uniformly distributed control bits on the second electrode layer 3;
and the control module is used for adjusting the voltage applied to the first electrode and the second electrode, so that a voltage difference is formed between adjacent control bits, and the liquid drop 7 is driven to move from the control bit at the current position to the next adjacent control bit until the preset position is moved.
Referring to fig. 1, in the present embodiment, the second electrodes are arranged transversely, the first electrodes are arranged longitudinally, and the control module can control the voltages applied to the respective first electrodes and second electrodes, control the voltages of the adjacent first electrodes to be different, and can drive the liquid drop to move transversely; the liquid drop can be driven to move longitudinally by the difference of the voltages of the adjacent second electrodes.
Optionally, the first electrode and the second electrode are both square and long, and the widths of the first electrode and the second electrode are the same;
the control positions are square, the diameter of the liquid drop 7 is not less than the side length of the control positions, so that the liquid drop 7 can cover one control position, and the liquid drop 7 can move between the adjacent control positions conveniently. I.e. the diameter of the droplet 7 is not smaller than the width of the first and second electrodes.
Optionally, the thermosensitive sensing layer 6 includes a plurality of sensors;
each sensor is used for storing position information corresponding to the sensor, converting heat into a voltage or current signal and sending the voltage or current signal and the position information to the control module;
and the control module is electrically connected with each sensor, is specifically used for comparing voltage or current signals of each sensor, determining the sensor corresponding to the position of the liquid drop 7, and determining the position information of the liquid drop 7 according to the position information of the sensor.
In practical application, the sensor may be a temperature sensor, and includes two series resistors, one of which is a thermistor, the resistance of the thermistor changes with heat, so that a voltage signal of the series resistor changes, and the voltage signal is amplified, filtered and AD-converted and then transmitted to the control module.
Optionally, the positions of the plurality of sensors correspond to the positions of the plurality of control bits one to one. In practical application, the control module stores position information of each control bit, the position information of each control bit corresponds to position information of one sensor, the position information of the sensor at the position of the liquid drop 7 is determined, and the position information of the control bit where the liquid drop 7 is located can be correspondingly determined according to the position information.
The control module is also used for planning the movement track of the liquid drop 7 according to the current position and the preset position of the liquid drop 7 and controlling the liquid drop 7 to move from one control position to another adjacent control position. Specifically, if the voltage of the adjacent first electrodes is adjusted to be different when the liquid drop 7 is laterally moved from one control bit to another adjacent control bit, the same voltage or no voltage can be applied to the second electrodes when the liquid drop 7 is laterally moved to the next control bit; if the liquid drop 7 moves longitudinally from one control bit to another adjacent control bit, the voltage of the adjacent second electrode can drive the liquid drop to move longitudinally.
Alternatively, the heat generating layer 2 is disposed between the first electrode layer 1 and the second electrode layer 3, the flow channel is located between the second electrode layer 3 and the heat sensitive layer 6, and the liquid droplet 7 moves in the flow channel.
Optionally, the top surface of the second electrode layer 3 is provided with a first insulating layer 4, and the bottom surface of the thermosensitive sensing layer 6 is provided with a second insulating layer 5; the flow channel is located between the first insulating layer 4 and the second insulating layer 5. The first insulating layer 4 and the second insulating layer 5 have an insulating function.
Optionally, in order to facilitate the movement of the droplets 7, the opposite faces of the first insulating layer 4 and the second insulating layer 5 are provided with hydrophobic layers; or the like, or, alternatively,
the material of the first insulating layer 4 and the material of the second insulating layer 5 are both hydrophobic materials. I.e. the first insulating layer 4 and the second insulating layer 5 fulfil the role of both insulating and hydrophobic.
In practice, the materials of the first insulating layer 4, the second insulating layer 5, and the second electrode layer 3 are required to have high thermal conductivity, so that heat loss during heat transfer is minimized, and heat source transfer can be performed uniformly, so that the heat can be maintained uniformly even after passing through the first insulating layer 4.
Referring to fig. 2, based on the above microfluidic device, an embodiment of the present application further provides a droplet control method, including the following steps:
and S1, controlling the heat generating layer 2 to generate heat uniformly when the liquid drops 7 are positioned between the heat generating layer 2 and the heat sensitive induction layer 6.
S2, receiving the heat electric signal output by the heat sensitive layer 6; wherein, the heat electric signal is obtained by converting the heat sensitive sensing layer 6 according to the sensed heat.
Alternatively, the process of outputting the thermal electric signal by the thermosensitive sensing layer 6 includes:
each sensor converts the sensed heat into a voltage or current signal and outputs the voltage or current signal and position information corresponding to the sensor. Wherein, the heat sensitive layer 6 comprises a plurality of sensors which are uniformly distributed; each sensor stores position information corresponding to the sensor.
S3, determining the current position of the droplet 7 according to the difference between the thermal electrical signals at the droplet 7 and the non-droplet 7.
Alternatively, in step S3, determining the current position of the droplet 7 according to the difference between the electrical heat signals at the droplet 7 and the non-droplet 7 includes:
comparing the received voltage or current signals to determine at least one voltage or current signal that is different from the other voltage or current signals;
and determining position information corresponding to the voltage or current signal according to the determined at least one voltage or current signal to obtain the current position of the liquid drop 7.
And S4, controlling the driving component to drive the liquid drop 7 to move to the preset position according to the current position of the liquid drop 7.
Alternatively, controlling the drive assembly to drive the droplet 7 to move to the predetermined position in dependence on the current position of the droplet 7 comprises:
different voltages are applied between the first electrode layer 1 and the second electrode layer 3 of the driving assembly, an electric field for driving the liquid droplet 7 to move is formed, and the liquid droplet 7 is driven to move to a preset position.
Alternatively, applying different voltages between the first electrode layer 1 and the second electrode layer 3 of the driving assembly to form an electric field for driving the droplet 7 to move, the driving the droplet 7 to move to a predetermined position comprising:
the voltages applied to the first and second electrodes are adjusted such that a voltage difference is formed between adjacent control bits, and the droplet 7 is driven to move from the control bit at the current position to the next adjacent control bit until a predetermined position is reached.
The first electrode layer 1 comprises a plurality of uniformly arranged first electrodes, and the second electrode layer 3 comprises a plurality of uniformly arranged second electrodes;
the projections of the plurality of first electrodes and the plurality of second electrodes in the same plane are vertical;
the intersections of the first and second electrodes form a plurality of evenly distributed control bits on the second electrode layer 3.
Alternatively, if the positions of the plurality of sensors correspond to the positions of the plurality of control bits one to one, controlling the driving assembly to drive the droplet to move to the predetermined position according to the current position comprises:
correspondingly determining the position of the control position where the liquid drop 7 is located based on the current position;
determining the position of the droplet 7 to be moved to the next adjacent control bit according to the position of the control bit where the droplet 7 is located;
according to the position of the control bit where the liquid drop 7 is located and the position of the control bit to be moved to the next adjacent control bit, finding a first electrode and a second electrode correspondingly;
the voltages applied to the first and second electrodes are adjusted such that a voltage difference is formed between adjacent control bits, and the droplet 7 is driven to move from the control bit at the current position to the next adjacent control bit until a predetermined position is reached.
Optionally, the specific adjustment process includes: if the liquid drop 7 moves from one control bit to another adjacent control bit, the voltage of the adjacent first electrodes can be adjusted to be different, and the liquid drop 7 is driven to move to the next control bit; if the liquid drop 7 moves longitudinally from one control bit to another adjacent control bit, the voltage of the adjacent second electrode can drive the liquid drop to move longitudinally.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The use of "first," "second," and similar terms in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
It will be understood that when an element such as a layer, film, region, or substrate is referred to as being "on" or "under" another element, it can be "directly on" or "under" the other element or intervening elements may be present.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.
The foregoing is only a partial embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (8)
1. A microfluidic device, comprising: the device comprises a thermosensitive induction layer, a heating layer for uniformly heating, a driving component for driving liquid drops to move and a control module;
a flow channel for containing liquid drops is arranged between the heating layer and the thermosensitive induction layer;
the heat-sensitive induction layer is used for inducing heat transferred by the heating layer and converting the heat into a heat electric signal to be output;
the control module is electrically connected with the thermosensitive sensing layer and used for receiving the thermal electric signal, determining the current position of the liquid drop according to the difference of the thermal electric signal at the liquid drop position and the thermal electric signal at the non-liquid drop position, and controlling the driving assembly to drive the liquid drop to move to the preset position according to the current position;
the heat-sensitive sensing layer comprises a plurality of sensors;
each sensor is used for storing position information corresponding to the sensor, converting the heat into a voltage or current signal and sending the voltage or current signal and the position information to the control module;
the control module is electrically connected with each sensor and used for comparing voltage or current signals of each sensor, determining the sensor corresponding to the position of the liquid drop, and determining the position information of the liquid drop according to the position information of the sensor;
the driving assembly comprises a first electrode layer and a second electrode layer which are arranged in parallel;
the first electrode layer comprises a plurality of uniformly arranged first electrodes, and the second electrode layer comprises a plurality of uniformly arranged second electrodes;
the projections of the plurality of first electrodes and the plurality of second electrodes in the same plane are vertical;
the intersections of the plurality of first electrodes and the plurality of second electrodes form a plurality of uniformly distributed control bits on the second electrode layer;
the control module is used for adjusting the voltage applied to the first electrode and the second electrode to form a voltage difference between adjacent control bits and drive the liquid drop to move from the control bit at the current position to the next adjacent control bit until the liquid drop moves to a preset position;
the positions of the sensors correspond to the positions of the control bits one to one.
2. The microfluidic device according to claim 1,
the second electrode layer is closer to the heat-sensitive sensing layer than the first electrode layer;
an electric field for driving the liquid drop to move is formed between the first electrode layer and the second electrode layer.
3. The microfluidic device according to claim 1, wherein the first electrode and the second electrode are each rectangular and elongated, and the width of the first electrode and the width of the second electrode are the same;
the control position is square, and the diameter of the liquid drop is not less than the side length of the control position.
4. The microfluidic device according to claim 2, wherein the heat generating layer is disposed between the first electrode layer and the second electrode layer, and the flow channel is disposed between the second electrode layer and the temperature sensitive layer.
5. The microfluidic device according to claim 4, wherein the top surface of the second electrode layer is provided with a first insulating layer, and the bottom surface of the thermosensitive sensing layer is provided with a second insulating layer;
the runner is located between the first insulating layer and the second insulating layer.
6. The microfluidic device according to claim 5, wherein the opposing surfaces of the first insulating layer and the second insulating layer are each provided with a hydrophobic layer; or the like, or, alternatively,
the material of the first insulating layer and the material of the second insulating layer are both hydrophobic materials.
7. A droplet control method applied to the microfluidic device according to any one of claims 1 to 6, comprising the steps of:
when the liquid drops are positioned between the heating layer and the thermosensitive induction layer, the heating layer is controlled to uniformly heat;
receiving a thermal electric signal output by the thermosensitive sensing layer, comprising: receiving voltage or current signals of each sensor of the thermosensitive sensing layer and position information of the sensor; the voltage or current signal is obtained by converting the sensor according to the sensed heat;
determining a current location of the droplet based on a difference in the electrical thermal signal at the droplet and at a non-droplet, comprising: comparing voltage or current signals of each sensor, determining a sensor corresponding to the position of the liquid drop, and determining the position information of the liquid drop according to the position information of the sensor to serve as the current position;
controlling a drive assembly to drive the droplet to move to a predetermined position according to the current position, comprising: and adjusting the voltage applied to the first electrode and the second electrode to form a voltage difference between adjacent control bits, and driving the liquid drop to move from the control bit at the current position to the next adjacent control bit until the liquid drop moves to the preset position.
8. The method of claim 7, wherein determining the current position of the droplet based on the difference in the electrical heat signal at the droplet and at the non-droplet comprises:
comparing the received voltage or current signals to determine at least one voltage or current signal that is different from the other voltage or current signals;
and according to the determined at least one voltage or current signal, determining position information corresponding to the voltage or current signal to obtain the current position of the liquid drop.
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| CN201910569444.XA CN110237877B (en) | 2019-06-27 | 2019-06-27 | Microfluidic device and droplet control method |
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| CN201910569444.XA CN110237877B (en) | 2019-06-27 | 2019-06-27 | Microfluidic device and droplet control method |
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| CN110237877A CN110237877A (en) | 2019-09-17 |
| CN110237877B true CN110237877B (en) | 2022-03-04 |
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