CN110237039A - A kind of paclitaxel freeze drying powder preparation technique and product - Google Patents
A kind of paclitaxel freeze drying powder preparation technique and product Download PDFInfo
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- CN110237039A CN110237039A CN201910649208.9A CN201910649208A CN110237039A CN 110237039 A CN110237039 A CN 110237039A CN 201910649208 A CN201910649208 A CN 201910649208A CN 110237039 A CN110237039 A CN 110237039A
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Abstract
The invention belongs to pharmaceutical preparations technology field, specially a kind of paclitaxel freeze drying powder preparation technique and product.Paclitaxel freeze drying powder preparation technique route of the invention is simple, and production cost is low, and transport, storage are easier, avoids addition excipient bring adverse reaction.The vitro stability of prepared taxol micelle freeze-drying powder is higher, and it is more than 12 hours that the paclitaxel solution after redissolution keeps the stable time at room temperature, meets the requirement of drug therapy.Blood stability is higher, partial size is smaller, is easier to play ERP effect.It can be redissolved with Normal Saline or glucose injection, enormously simplify administration process.
Description
Technical field
The invention belongs to pharmaceutical preparations technology fields, and in particular to a kind of paclitaxel freeze drying powder preparation technique and product.
Background technique
Taxane molecule formula are as follows: C47H51NO14, molecular weight 853.9,213~216 DEG C of fusing point, with highly lipophilic property, no
It is dissolved in water, is the natural anti-cancer drugs of isolated a kind of efficient, low toxicity from Chinese yew genus plants, wide spectrum.It is red bean
The natural secondary metabolin of one of genus Taxus complexity, molecular structure see below formula.
Taxol is the drug of a kind of micro-pipe specificity having compared with high anti-cancer activity, and sales volume ranks anti-cancer agent throughout the year
The umber one, and with 20% speed increase.
1977, Horwit Z discovery taxol anticancer mechanism was that tubulin can be promoted with tubulin binding
Polymerization is assembled into micro-pipe dimer, to inhibit the normal physiological depolymerization of micro-pipe in cell, cell mitogen is made to stop at G2
Phase and M phase prevent the quick breeding of cancer cell.Further study show that taxol can activating macrophage, lead to tumour
The reduction and its release of mecrosis factor receptors, killing or inhibition tumour cell；Taxol can induce cell apoptosis, and cause cell
Programmed death；Taxol can also inhibit the migration of tumour cell.Clinical research shows that taxol has wide spectrum and efficiently resists
Cancer activity, prominent for treatment oophoroma, breast cancer, uterine cancer, gastric cancer etc. curative effect, recent research finds it to rheumatoid
Property arthritis, senile dementia etc. also have certain curative effect.
Since taxol is insoluble in water, taxol is usually dissolved in Emulsifier EL-60 in current clinical application
To increase its water solubility in (cremophor EL) and dehydrated alcohol mixed solvent.Ethyl alcohol has certain cytotoxicity, and gathers
Histamine is discharged when ethylene oxide castor oil degradation in vivo, leads to different degrees of allergic reaction, can also cause interior, nerve cell
Grain release and disentwining angle velocity and aggravate the peripheral nerve toxicity of taxol.
Its curative effect is improved to reduce toxicity, clinically develops the novel form of taxol successively in recent years.Wherein without poly-
Ethylene oxide castor oil injection taxol albumin nano suspension (ABI-007) at home and abroad lists.With without antiallergy
Pretreatment, the features such as curative effect is preferable, toxicity is lower；Now just the Paclitaxel liposome of clinical research China research and development is carried out at home
Start in clinical application and the pro-drug (DHA-PTX) and polymeric dosage form genexol-PM and xyotax of taxol also just
In the research of preclinical and clinical I-III phase, it is shown that good prospect.
But recent study is found, the biggish polymer micelle of the partial size either formed by heavy polymer, also
It is the lesser polymer micelle of partial size formed by low-molecular weight polymer, has the shortcomings that blood stability is poor, micella exists
Almost immediately disintegrate and discharge the drug of carrying after into blood, the distribution of the drug that final two kinds of micellas carry in vivo is almost
It is not different, does not show ERP effect.Savic R's et al. the study found that taxol micella enters after blood usually 5
90% drug release is just had more than in minute and toward tissue diffusion (see Langmuir 2006,22:3570-8.
《Assessment of the integrity of poly(caprolactone)-b-poly(ethylene oxide)
micelles under biological conditions:a fl uorogenic-based approach.》)。
This result and imagination are reached tumor locus and are passed through EPR effect release drug again by micella particle carrying drug
Perfect condition greatly differs from each other, i.e., non-mature release (Premature release).The reason of leading to non-mature release on the one hand by
Micella is caused to disintegrate lower than its critical micelle concentration (CMC concentration) in concentration of the micella after hemodilution, another aspect blood
A large amount of existing protein can have an effect with micella and cause it to reunite and discharge drug in liquid.Therefore, it is injected intravenously in design
Its blood stability must be considered when cancer target nano-carrier first, is only just expected under the premise of improving blood stability
The targeting of drug is improved using EPR effect.
In addition, existing taxol micellar preparation has shortcoming.One is process conditions are more complex, large-scale industry
It produces difficult；The second is ethyl alcohol need to be used, with cytotoxicity.For example, " the Japanese yew of Lanzhou University Liu Weisheng et al. invention
Alcohol-polymer medicament carrying micelle preparation process " (number of patent application 201110255867.8) needs the technique item using constant temperature oscillation
Part；Peking University Lv Wanliang et al. invention " a kind of paclitaxel nano micelle and its application " (application number:
201110044677.1), need the pH value for keeping reaction environment using buffer to stablize during the preparation process.It is in glue simultaneously
Contain a certain amount of ethyl alcohol in beam, there is certain cytotoxicity.Li Lingbing of Shandong University et al. has invented a kind of " taxol
Mixed micelle preparation and preparation method thereof " (number of patent application 201110139712.8), formula in also to use ethyl alcohol.
Freeze-dried powder preparation can effective preservation drug.Freeze-dried powder is made contained by it by that will need dry product at low temperature
Moisture freezes, and is then placed on drying in the environment of vacuum, moisture is allowed directly to be distilled by solid state for vapor and from product
It excludes and makes product activity is dry to obtain.Freeze-dried powder can be effectively prevented the change of product physics and chemistry and biological nature, effective protection
The stability of thermal sensitivity medicament effective component；Form is loose after the drying, color does not change substantially for it, adds water or hydrophilic
The physicochemical property and bioactivity of original aqueous solution can be quickly dissolved and restored after property organic solvent；Due to dry in vacuum item
It is carried out under part, there is good protective effect for some oxidizable substances；Product water content after freeze-drying is very low,
Improve the stability of product, contaminated chance reduces, this not only facilitates transport and also extends the product pot-life.
Since taxol is not soluble in water, there is a problem of paclitaxel injection, partial size is bigger than normal with water redissolution and after redissolving,
Therefore less to the research achievement of paclitaxel injection maturation at present.Wherein Liu Tong et al. is in " solid nano-medicine and its preparation
Method " " a kind of preparation method of paclitaxel injection ", the paclitaxel freeze drying pointed out are disclosed in (CN100518831)
Powder adds water (or solvent for injection) that particle is precipitated because solubility in water reduces when dissolving.Scape is far refined et al. in " taxol
One kind is disclosed in lyophilized powder injection of polymer bond drug and preparation method thereof " (number of patent application 200610016614)
The preparation method of paclitaxel injection, the more complex purple for needing to add a large amount of stabilizer and cosolvent, and obtaining of technique
Partial size is larger after China fir alcohol freeze-dried powder redissolves, and is 100-150nm.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, using mPEG-PLA- phenylalanine as package Japanese yew
The auxiliary material of alcohol drug, the mPEG-PLA- phenylalanine molecular formula are as follows:
Wherein a=20-200, b=5-300.
Paclitaxel freeze drying powder preparation technique of the present invention comprises the following steps
1. weighing raw material (taxol, mPEG-PLA- phenylalanine) according to different feed ratios；
2. above-mentioned raw materials are put into container, at a temperature of 15-45 DEG C, the organic solvents such as ethyl alcohol or acetonitrile are added to complete
Dissolution, course of dissolution can be using the means such as stirring or oscillation.
3. by above-mentioned solution in 30-50 DEG C of rotary evaporation 2h, until organic solvent distilled-to-dryness.Vacuum at 10-40 DEG C subsequent
Dry > 12h removes remaining organic solvent, obtains the mixed with polymers film containing taxol；
4. the ultrapure water of identical preheating temperature is added or life is added by above-mentioned hybrid films in 40-60 DEG C of water-bath to transparence
Salt water, phosphate buffer are managed, shake well aquation obtains transparent polypeptide drug-loaded micelle solution.The polypeptide drug-loaded micelle solution is used
0.45 μm of filtering with microporous membrane；
5. above-mentioned filtered polypeptide drug-loaded micelle solution is lyophilized, paclitaxel injection is obtained.
Preferably, freeze-drying of the present invention, includes the following steps.
A. be put into polypeptide drug-loaded micelle solution after being cooled to -3~0 DEG C in advance in vacuum freezing drying oven, fast cooling to -40 DEG C with
Under, maintain 2h or more.
B. after ensuring polypeptide drug-loaded micelle solution temperature lower than -40 DEG C, vacuum pump is opened.Final vacuum is set as
0.015Bar.Vacuumize 1h or more, it is ensured that vacuum degree is lower than 0.02Bar in vacuum freezing drying oven.
C. in the case where keeping vacuum state, start the temperature in Raise vacuum freeze drying box.First internal temperature is set as
- 20 DEG C, maintain 10h or more.Internal temperature is then set as 0 DEG C, maintains 10h or more.Then shelf temperature is set as 5
DEG C, maintain 3h or more.Internal temperature is subsequently set as 20 DEG C, maintains 4h or more, it is ensured that products temperature is tied after reaching 20 DEG C
Beam freeze-drying.
Preferably, heretofore described mPEG-PLA- phenylalanine, the molecular weight of methoxypolyethylene glycol block are 1000
~3000；The molecular weight of polylactide block is 500~5000.
In the present invention, mPEG-PLA- phenylalanine is without apparent carcinogenicity, no genotoxicity, no teratogenesis, mutagenicity,
Degradable in vivo is lactic acid, PEG, phenylalanine, can be expelled directly out external.Acute toxicity test, mouse are carried out to mouse
LD50 > 2.00g/kg；In long term toxication, 1.00g/kg dosage, 1 times/day, successive administration 2 days, are discontinued 5 days, continuously weekly
After being administered 13 weeks, is observed after restoring 4 weeks, have no obvious toxic-side effects.Cytotoxicity test shows mPEG-PLA- phenylalanine
Cytotoxicity it is lower than the block copolymer Mpeg-PLA of current generally recognized as safe.
Another object of the present invention is to provide according to the obtained product of paclitaxel freeze drying powder preparation technique, the production
Object is the lyophilized preparation containing Japanese yew alcohol component, i.e. paclitaxel injection；In the paclitaxel injection contained taxol and
The weight ratio of mPEG-PLA- phenylalanine is (2.87~27.66): 100；After the paclitaxel injection redissolves, purple is measured
The average grain diameter of China fir alcohol micella is 20.8~35.2nm, and the stability under room temperature in blood plasma is greater than 12h.
Compared with prior art, the invention has the following advantages:
1, by the selection of the auxiliary material to package taxol, so that preparation process is simplified.
Even if 2, not adding excipient in freeze-drying process, the preferable performance of freeze-dried powder can be also kept, addition is both avoided and assigns
Shape agent reduces production cost to medication bring adverse reaction.
3, the molecular weight of mPEG-PLA- phenylalanine changes in preparation process, and prepared paclitaxel injection is multiple
Change of size after molten is smaller.
4, it is more than 12 hours that prepared taxol micelle freeze-drying powder keeps the stable time at room temperature after redissolving, and is met
The requirement of drug therapy.
5, prepared taxol micelle freeze-drying powder Normal Saline or glucose injection can redissolve, significantly
Administration process is simplified, blood stability is higher, partial size is smaller, is easier to play ERP effect.
Detailed description of the invention
Nothing.
Specific embodiment
The present invention is described in detail below with reference to embodiment, the present embodiment is under the premise of the technical scheme of the present invention
Implemented, the detailed implementation method and specific operation process are given, but protection scope of the present invention be not limited to it is following
Embodiment.The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment (1-7)
The preparation method of composition includes the following steps: in the present invention
1. weighing taxol and mPEG-PLA- phenylalanine as raw material according to different feed ratios (feed ratio is shown in Table 1).
Wherein, taxol (CAS 33069-62-4) is produced by Xi'an Rayleigh Biotechnology Co., Ltd, and purity is greater than 95%, mPEG-
PLA- phenylalanine is voluntarily prepared as inventor according to technique described in patent No. PCT-CN-2013000453；
2. above-mentioned raw materials are put into container, at a temperature of 15-45 DEG C, the organic solvents such as ethyl alcohol or acetonitrile are added to complete
Dissolution.Course of dissolution can be using the means such as stirring or oscillation.
3. by above-mentioned solution in 30-50 DEG C of rotary evaporation 2h, until organic solvent distilled-to-dryness.Vacuum at 10-40 DEG C subsequent
Dry > 12h removes remaining organic solvent, obtains the mixed with polymers film containing taxol.
4. the ultrapure water of identical preheating temperature is added or life is added by above-mentioned hybrid films in 40-60 DEG C of water-bath to transparence
Salt water, phosphate buffer are managed, shake well aquation obtains transparent polypeptide drug-loaded micelle solution.The polypeptide drug-loaded micelle solution is used
0.45 μm of filtering with microporous membrane.
5. above-mentioned filtered polypeptide drug-loaded micelle solution is lyophilized in the steps below:
A. be put into polypeptide drug-loaded micelle solution after being cooled to -3~0 DEG C in advance in vacuum freezing drying oven, fast cooling to -40 DEG C with
Under, maintain 2h or more.
B. after ensuring polypeptide drug-loaded micelle solution temperature lower than -40 DEG C, vacuum pump is opened.Final vacuum is set as
0.015Bar.Vacuumize 1h or more, it is ensured that vacuum degree is lower than 0.02Bar in vacuum freezing drying oven.
C. in the case where keeping vacuum state, start the temperature in Raise vacuum freeze drying box.First internal temperature is set as
- 20 DEG C, maintain 10h or more.Internal temperature is then set as 0 DEG C, maintains 10h or more.Then shelf temperature is set as 5
DEG C, maintain 3h or more.Internal temperature is subsequently set as 20 DEG C, maintains 4h or more, it is ensured that products temperature is tied after reaching 20 DEG C
Beam freeze-drying.
In lyophilized technique, the solution for being difficult to solid state can add excipient to ensure to finally obtain solid-state powder
End.And various excipient can also be different degrees of the performance indicators such as appearance, form, dissolubility, the stability of improvement lyophilized preparation.
Inventor attempts the excipient such as lactose, glycine, glucose in above-mentioned technique, but experimental result does not find addition excipient
Have significantly for reducing the stability etc. that solution is redissolved in technology difficulty, the form for improving final preparation and promotion beneficial to effect
Fruit.Addition lactose and dextrose excipient have some improvement effect, but this beneficial effect for the appearance of freeze-dried powder preparation
For medicinal application whether it is valuable then be worth discussion.
Embodiment 1-7 selects different feed ratios respectively, and selects the mPEG-PLA- of different block molecule amounts respectively
Paclitaxel injection is made in phenylalanine through the above steps.Using the content of HPLC method measurement taxol after redissolution, and calculate
It obtains its drugloading rate, the results are shown in Table 1.
Table 1: the load medicine data of different embodiments
Wherein, auxiliary material=mPEG-PLA- phenylalanine
Freeze-dried powder is used water for injection, physiological saline, glucose water equal solvent redissolve respectively, it is pure and strong to obtain Japanese yew after dilution
Degree is about the solution of 3mg/ml.It is measured with dynamic light scattering, redissolves the particle diameter distribution of solution between 10-100nm, average grain diameter
Between 20.8~35.2nm nm (being shown in Table 2).Under normal indoor illumination condition, respectively will redissolve solution be placed in 15 DEG C, 25 DEG C,
30 DEG C of isoperibols visually observe the stability of solution every 2h, until discovery solution muddy or discovery occurs and has precipitating to analyse
Out, then illustrate that solution finishes stable state.It the results are shown in Table 2.
Table 2: different embodiment freeze-dried powders redissolve test result
Freeze-dried powder made from embodiment 1-7 is subjected to stability test.The method of stability test is respectively to set sample
Under the conditions of 25 DEG C, relative humidity 60%RH and 6 ± 2 DEG C, under the conditions of relative humidity 60%RH, its property of period sampling measuring
Shape, average grain diameter, pH value, loss on drying, redissolves the items indexs such as stability of solution at content of taxol.By 180 days stabilizations
Property test, institute's mark has no significant change, and shows that character of the invention is continual and steady.
The blood stability of the comparison present invention and mpeg-pla taxol micella, freeze-dried powder made from embodiment 1-7 is used
Ultrapure water redissolution is diluted to after content of taxol is 3mg/ml, is added the blood plasma of 50wt%, and with same paclitaxel concentration
Mpeg-pla taxol micella is as control, the time needed for measuring particle diameter distribution coefficient (PDI) > 0.3 using dynamic light scattering,
As a result such as table 3.The result shows that the taxol micella blood in the present invention, which stablizes the time, is significantly higher than mpeg-pla taxol micella.
Table 3: blood stability test
| Embodiment | The time required to PDI > 0.3 (h) |
| Mpeg-pla control | 13 |
| Embodiment 1 | 34 |
| Embodiment 2 | 38 |
| Embodiment 3 | 49 |
| Embodiment 4 | 42 |
| Embodiment 4 | 36 |
| Embodiment 5 | 28 |
| Embodiment 6 | 26 |
| Embodiment 7 | 22 |
Claims (6)
1. a kind of paclitaxel freeze drying powder preparation technique, which is characterized in that comprise the following steps:
The first step weighs taxol and mPEG-PLA- phenylalanine as raw material according to different feed ratios；
Second step puts into above-mentioned raw materials in container, and at a temperature of 15-45 DEG C, organic solvent is added to being completely dissolved.It dissolved
Journey can be using the means such as stirring or oscillation；
Third step, by above-mentioned solution in 30-50 DEG C of rotary evaporation 2h, until organic solvent distilled-to-dryness.Vacuum at 10-40 DEG C subsequent
Dry > 12h removes remaining organic solvent, obtains the mixed with polymers film containing taxol；
4th step is added the ultrapure water of identical preheating temperature or life is added by above-mentioned hybrid films in 40-60 DEG C of water-bath to transparence
Salt water, phosphate buffer are managed, shake well aquation obtains transparent polypeptide drug-loaded micelle solution.The polypeptide drug-loaded micelle solution is used
0.45 μm of filtering with microporous membrane；
Above-mentioned filtered polypeptide drug-loaded micelle solution is lyophilized, obtains paclitaxel injection by the 5th step；
Wherein, mPEG-PLA- phenylalanine molecular formula is as follows:
Wherein a=20-200, b=5-300.
2. a kind of paclitaxel freeze drying powder preparation technique as described in claim 1, it is characterised in that: the organic solvent is ethyl alcohol
Or acetonitrile.
3. a kind of paclitaxel freeze drying powder preparation technique as described in claim 1, it is characterised in that: the mPEG-PLA- phenylpropyl alcohol
In propylhomoserin, the molecular weight of polylactide block is 500~5000, and the molecular weight of methoxypolyethylene glycol block is 1000~3000.
4. a kind of paclitaxel freeze drying powder preparation technique as described in claim 1, which is characterized in that the freeze-drying includes following step
It is rapid:
The first step is put into polypeptide drug-loaded micelle solution after being cooled to -3~0 DEG C in advance in vacuum freezing drying oven, fast cooling is to -40 DEG C
Hereinafter, maintaining 2h or more；
Second step, it is ensured that after polypeptide drug-loaded micelle solution temperature is lower than -40 DEG C, open vacuum pump.Final vacuum is set as
0.015Bar.Vacuumize 1h or more, it is ensured that vacuum degree is lower than 0.02Bar in vacuum freezing drying oven；
Third step starts the temperature in Raise vacuum freeze drying box in the case where keeping vacuum state.First internal temperature is set
It is -20 DEG C, maintains 10h or more.Internal temperature is then set as 0 DEG C, maintains 10h or more.Then shelf temperature is set as 5
DEG C, maintain 3h or more.Internal temperature is subsequently set as 20 DEG C, maintains 4h or more, it is ensured that products temperature is tied after reaching 20 DEG C
Beam freeze-drying.
5. a kind of obtained paclitaxel injection of paclitaxel freeze drying powder preparation technique according to claim 1, feature
Be: in the paclitaxel injection weight ratio of contained taxol and mPEG-PLA- phenylalanine be (2.87~
27.66): 100.
6. a kind of obtained paclitaxel injection of paclitaxel freeze drying powder preparation technique according to claim 1, feature
Be: after the paclitaxel injection redissolves, the average grain diameter of micella is 20.8~35.2nm in solution.
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| GB2542092B (en) * | 2014-07-15 | 2019-05-29 | Teng Xin | Polyethylene glycol methyl ether-polylactide-lysine micellar compositions comprising docetaxel |
| CN109288813B (en) * | 2017-07-24 | 2021-03-12 | 复旦大学 | Selenium-containing taxol dimer prodrug polymer nanoparticles and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101618013A (en) * | 2009-07-28 | 2010-01-06 | 四川大学 | Focused ultrasound-polymeric micelle controllable drug release device and release method thereof |
| CN103342788A (en) * | 2013-06-09 | 2013-10-09 | 中国科学院上海药物研究所 | Triblock polycation, and preparation method and application thereof |
| WO2014039012A1 (en) * | 2012-09-06 | 2014-03-13 | Nanyang Technological University | Hyaluronic acid-based drug delivery systems |
| CN103768013A (en) * | 2014-01-17 | 2014-05-07 | 丽珠医药集团股份有限公司 | Paclitaxel polymer micelle by using refined amphiphilic block copolymer as carrier |
| CN103772686A (en) * | 2012-10-26 | 2014-05-07 | 苏州雷纳药物研发有限公司 | Amphiphilic block copolymer and preparation method thereof, micelle drug delivery system formed by copolymer and anti-tumor drug |
-
2014
- 2014-05-10 CN CN201910649208.9A patent/CN110237039A/en active Pending
- 2014-05-10 CN CN201410196752.XA patent/CN104511022A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101618013A (en) * | 2009-07-28 | 2010-01-06 | 四川大学 | Focused ultrasound-polymeric micelle controllable drug release device and release method thereof |
| WO2014039012A1 (en) * | 2012-09-06 | 2014-03-13 | Nanyang Technological University | Hyaluronic acid-based drug delivery systems |
| CN103772686A (en) * | 2012-10-26 | 2014-05-07 | 苏州雷纳药物研发有限公司 | Amphiphilic block copolymer and preparation method thereof, micelle drug delivery system formed by copolymer and anti-tumor drug |
| CN103342788A (en) * | 2013-06-09 | 2013-10-09 | 中国科学院上海药物研究所 | Triblock polycation, and preparation method and application thereof |
| CN103768013A (en) * | 2014-01-17 | 2014-05-07 | 丽珠医药集团股份有限公司 | Paclitaxel polymer micelle by using refined amphiphilic block copolymer as carrier |
Non-Patent Citations (1)
| Title |
|---|
| 单虎等: "《现代兽医兽药大全》", 30 April 2011, 中国农业大学出版社 * |
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