CN110215884A - A kind of gel for electrophoresis preparation facilities - Google Patents
A kind of gel for electrophoresis preparation facilities Download PDFInfo
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- CN110215884A CN110215884A CN201910592660.6A CN201910592660A CN110215884A CN 110215884 A CN110215884 A CN 110215884A CN 201910592660 A CN201910592660 A CN 201910592660A CN 110215884 A CN110215884 A CN 110215884A
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- offset plate
- gel
- preparation facilities
- cavity
- comb teeth
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/0052—Preparation of gels
- B01J13/0065—Preparation of gels containing an organic phase
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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Abstract
The invention discloses a kind of gel for electrophoresis preparation facilities, including comb teeth, upper offset plate and lower offset plate, upper offset plate mutually to fasten with lower offset plate, and forms the open-topped cavity for accommodating colloid；Comb teeth includes rack gear and comb body, and comb teeth is covered in the top opening of cavity, and rack gear is towards being arranged in cavity；The liquid leakage tank of a rectangular shape is provided on comb body along its length, is provided with channel between rack gear, channel is communicated with liquid leakage tank；The present invention on comb body by being arranged liquid leakage tank, so that extra gel is introduced liquid leakage tank, colloid is avoided to expand and cause deformation after comb teeth is in insertion gel.
Description
Technical field
The invention belongs to gel electrophoresis equipment technology fields, and in particular to a kind of in polyacrylamide gel electrophoresis
Gel for electrophoresis preparation facilities.
Background technique
Gel electrophoresis (English: Gel electrophoresis) or colloid electrophoresis are a major class technologies, by research-on-research
Person is used to separate the molecule of different physical properties (such as size, shape, isoelectric point).Wherein polyacrylamide gel electrophoresis abbreviation
It is one using polyacrylamide gel as supporting dielectric for PAGE(Polyacrylamide gel electrophoresis)
The common electrophoretic techniques of kind, technique of polyacrylamide gel electrophoresis are most common technologies in Separation of Proteins and identification.Carry out
Charged particle can carry out counter electrode migration under the influence of externally-applied magnetic field when electrophoretic separation, and the movement of particle is by gel media around
Interference and slow down, form molecular sieve；Under the joint effect of electric field force and the adverse effect of molecular sieve, in test sample
Protein expression goes out different migration rates, can estimate its molecule matter in the relative migration of standard PAGE gel by protein
Amount, and the single band of gel can be used as the standard for judging lipidated protein.
Currently, most of electrophoresis carries out in the Vertial electrophorestic tank for the plastic plate being made of two pieces of glass plates, market at present
On plastic plate be substantially and fixed without fixed device or two sides, plastic plate when carrying out encapsulating front plate, do not allow by two boards
It is easily perfectly aligned, easily cause leakage.Usually before encapsulating, the topped up with water into the cavity of the formation of two blocks of offset plates is needed, to verify it
Leakproofness, it is cumbersome, and the remaining water stain effect that will affect electrophoresis, even if buckle knot is arranged in prefabricated offset plate two sides at present
Structure equally will cause bottom leakage to complete the fixation of two blocks of offset plates, but in encapsulating clamping plate.
In addition, during encapsulating, it may occur that the gluey volume of the expansion of gel colloidal, gel-forming can be more original molten
Liquid product is more, and whole gel process is first solidified by the glue of lower end high concentration, and therefore, the colloid that lower end first solidifies can be right
The not solidified low concentration glue in upper end generates an extrusion process, if comb teeth and the compatible degree of upper end opening are poor, after expansion
Gel colloidal easily squeezes comb teeth, causes the tilt variation in comb teeth home position, and the perforation formed on gel is destroyed；Comb teeth with it is upper
The compatible degree of end opening is preferable, then the gel of low concentration above can be caused to be extruded (because the gel compressibility of low concentration is than high
Concentration is good), after comb teeth of being chosen using gel, the reserved perforation interval of gel be will be shifted up, to cause subtracting for perforation volume
It is few.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of gel for electrophoresis in polyacrylamide gel electrophoresis
Liquid leakage tank is arranged in preparation facilities on comb body, so that extra gel is introduced liquid leakage tank, is kept away after comb teeth is in insertion gel
Exempt from colloid expansion and causes deformation.
Technical solution provided by the invention is as follows:
A kind of gel for electrophoresis preparation facilities, is made of offset plate and comb teeth, and offset plate includes upper offset plate and lower offset plate, and upper offset plate is under
Offset plate mutually fastens, and forms the open-topped cavity for accommodating colloid；Comb teeth includes rack gear and comb body, and comb teeth is covered in chamber
In the top opening of body, rack gear is towards being arranged in cavity；The leakage of a rectangular shape is provided on comb body along its length
Liquid bath is provided with channel between rack gear, and channel is communicated with liquid leakage tank.
Preferably, bottom and two sides after upper offset plate is fastened with lower offset plate are fixedly connected, the lower end of upper offset plate or lower offset plate
Two or more open slots are provided with, open slot is communicated with inside cavity, for connection electrode slot and as electrophoresis conduction
The under shed of mouth, open slot are equipped with removable sealing strip.
It is highly preferred that being fixed between upper offset plate and lower offset plate by ultrasonic bonding.
It is highly preferred that upper offset plate or lower offset plate two sides are equipped with several limited posts, for defining the thickness of ultrasonic bonding.
Preferably, two "u"-shapeds are respectively provided on the two sides of upper offset plate, the open end of two "u"-shapeds is opposite
It is arranged, accordingly sets and buckled into there are two rectangular protrusion A, raised A in the gap of two "u"-shapeds formation on lower offset plate.
Preferably, one side opposite with liquid leakage tank on comb body is provided along its length the protrusion of a rectangular shape
B, the thickness of raised B offset plate consistency of thickness adjacent thereto.
Preferably, several grooves are provided along its length in the inside of the offset plate of the same side with liquid leakage tank, groove exists
Vertical height on offset plate is greater than the vertical height for pouring into gel in cavity, lower than in insertion cavity comb teeth top end it is vertical
Highly.
It is highly preferred that the shape of groove can be circle, diamond shape, square or rectangular.
Compared with prior art, the present invention has following technical advantage:
(1) three face of gel for electrophoresis preparation facilities in the present invention is fixed, only top opening, upper and lower offset plate combine it is stronger,
The leakproofness of whole device is increased, meanwhile, the deformation that upper and lower offset plate is generated by extruding is reduced to the full extent, is guaranteed good
Good electrophoresis environment；
(2) offset plate up and down in the present invention uses ultrasonic bonding, and the object of two need welding is transmitted to using high-frequency vibration wave
Surface makes two body surface phase mutual friction in the case where pressurization and forms the fusion between molecular layer.With traditional welding
Technique is compared, and welding process is stablized, and weld strength is high, obtained offset plate good airproof performance, no leakage, cleanliness without any pollution；
(3) present invention is provided with multiple open slots in the lower end of upper offset plate or lower offset plate, is used for electricity outlet, open slot and upper offset plate
Cavity perforation between lower offset plate, increases the rigidity of whole device, if being provided only with an open slot, the clip of electrophoresis tank is solid
When the upper offset plate or lower offset plate that are scheduled on around open slot, easily lead to the rupture of gel for electrophoresis preparation facilities, and be arranged two or
There is interval between open slot in more than two open slots, force dispersion, upper offset plate or lower offset plate do not allow easy fracture；Similarly, because
For the dispersion of stress, the setting of open slot can also be effectively prevented from gel obtained to be caused due to stress squeezes during transportation
Problem on deformation generation；
(4) present invention is provided with a liquid leakage tank on comb body, by the channel reserved between the rack gear that communicates with this, introduces because coagulating
Glue volume expansion and the partial gel liquid being extruded, the deformation for avoiding the colloid in gel preparation facilities from causing by expansion；It can protect
The card later period effectively cuts off gel after transfering to comb teeth, and the reserved perforation interval of gel not will be shifted up, and will not cause
The reduction of perforation volume；
(5) present invention is provided with the raised B of a rectangular shape on comb body, when comb teeth is inserted into the intracorporal certain position of chamber
Afterwards, raised B is flushed with adjacent offset plate edge, at this point, comb teeth will not continue to extend into cavity, has the work for determining comb teeth position
With；
(6) present invention and liquid leakage tank on the inside of it, are provided along its length several grooves on the offset plate of the same side, should
Groove can equally accommodate the partial gel liquid being extruded by gel volume expansion, also can avoid the gel in gel preparation facilities
The deformation that body causes by expansion.
Detailed description of the invention
Fig. 1 is the positive structure diagram of upper offset plate in the present invention
Fig. 2 is the backsight structural representation of upper offset plate in the present invention
Fig. 3 is the schematic perspective view of lower offset plate in the present invention
Fig. 4 is the positive structure diagram of comb teeth in the present invention
Fig. 5 is the backsight structural representation of comb teeth in the present invention
Fig. 6 is the positive structure diagram of lower offset plate in another embodiment in the present invention
Wherein: lower 4. open slot of offset plate, 5. limited post, 6. "u"-shaped protrusion, the 7. protrusion A 8. of offset plate 3. on 1. comb teeth 2.
11. channel of rack gear 9. comb body, 10. liquid leakage tank, 12. protrusion 13. groove of B.
Specific embodiment
First with specific embodiments and the drawings, the present invention is described further.
As shown in Fig. 1 ~ 5, the invention discloses a kind of gel for electrophoresis preparation facilities, including comb teeth 1, upper offset plate 2 and under
Offset plate 3, lower offset plate 3 are rectangular shape, and upper offset plate 2 is in " recessed " shape, the entire length and width of upper offset plate 2 and lower stirring 3
Unanimously, it after upper offset plate 2 is mutually fastened with lower stirring 3, is fixed by ultrasonic bonding, and form open-topped accommodate
The cavity (not marked in figure) of colloid；In the lower end of upper offset plate 3, along its length, if there are four open slot 4, open slot 4 with
Inside cavity communicates, and the under shed for connection electrode slot and as electrophoresis conduction mouth, open slot 4 connects setting in a row, it
Between interval it is equal, open slot 4 is equipped with removable sealing strip (not marking in figure)；In lower offset plate 3 along its width direction
On two sides, two cylindrical limited posts 5 are respectively equipped with, the height of limited post 5 represents the thickness of ultrasonic bonding.
After guaranteeing that upper offset plate 2 and lower offset plate 3 fasten, left and right does not occur between the two or sways forwards and backwards, the present invention exists
Upper offset plate 2 is respectively provided with two "u"-shaped protrusions 6 along the two sides of its width direction, and two "u"-shaped protrusions 6 are opened
Mouthful end is oppositely arranged, correspondingly, on the two sides on lower offset plate 3 along its width direction, be respectively provided with one it is rectangular convex
A7 is played, raised A7 is buckled into the gap that two "u"-shaped protrusions 6 are formed.
Comb teeth 1 in the present invention includes rack gear 8 and comb body 9, and comb teeth 1 is covered in the top opening of cavity, 8 direction of rack gear
Setting in cavity；The length of comb teeth 1 is less than the length for the groove-like structure that upper offset plate 2 is formed by " recessed " shape, in order to accommodate because
Gel volume expands and the extra coagulant liquid that is extruded, the present invention be provided with along its length on comb body 9 one it is rectangular
The liquid leakage tank 10 of shape is provided with channel 11 between rack gear 8, and channel 11 is communicated with liquid leakage tank 10, is extruded rear extra solidifying
Glue can enter in liquid leakage tank 10 along channel 11, the deformation without causing formed colloid.
In order to guarantee that position of the comb teeth 1 in insertion colloid is fixed, the present invention is opposite with liquid leakage tank 10 one on comb body 9
Face is provided along its length the raised B12 an of rectangular shape, the thickness of raised B12 and offset plate consistency of thickness thereon, when
Comb teeth 1 is in insertion cavity after certain position, and raised B12 is flushed with gluing edges of boards edge, at this point, comb teeth 1 will not continue to be deep into
In cavity, the later period may make in operation, the position that comb teeth 1 is inserted into gel every time is all identical.
As shown in fig. 6, the present invention can also exist with liquid leakage tank 10 as another preferred embodiment in the present invention
The inside of the lower offset plate 3 of the same side is provided along its length several grooves 13, vertical height of the groove 13 on lower offset plate 3
Degree is greater than the vertical height for pouring into gel in cavity, lower than the vertical height of 1 top end of comb teeth in insertion cavity, the groove 13
The partial gel liquid that is extruded due to gel volume expansion can equally be accommodated, also can avoid gelinite in gel preparation facilities because
Expand the deformation caused.
When actually using the gel preparation facilities in the present invention, without cleaning, the acrylamide heavy-fluid that will be prepared in advance
And light liquid, it pours into the cavity in gel preparation facilities, after defined gelinite height to be fed, comb teeth 1 is inserted into gelinite
In, 10 structure of liquid leakage tank on comb teeth 1 and 13 structure of groove on offset plate can effectively prevent the deformation of gelinite, pull up before electrophoresis
Sealing strip on comb teeth 1 and open slot 4, offset plate is put into electrophoretic apparatus, can be loaded, electrophoresis.
It should be noted that the quantity of the rack gear 8 in the structural schematic diagram of comb teeth 1 of the present invention is eight, but do not represent reality
Border produces the quantity of hour rack, and the quantity of conventional rack gear 8 is 8 ~ 20.
Claims (8)
1. a kind of gel for electrophoresis preparation facilities, is made of offset plate and comb teeth, offset plate includes upper offset plate and lower offset plate, upper offset plate with
Lower offset plate mutually fastens, and forms the open-topped cavity for accommodating colloid；Comb teeth includes rack gear and comb body, and comb teeth is covered in
In the top opening of cavity, rack gear is towards being arranged in cavity；It is characterized by: being provided with along its length on comb body one long
The liquid leakage tank of cube shape is provided with channel between rack gear, and channel is communicated with liquid leakage tank.
2. a kind of gel for electrophoresis preparation facilities according to claim 1, it is characterised in that: upper offset plate and lower offset plate fasten
Bottom and two sides afterwards is fixedly connected, and the lower end of upper offset plate or lower offset plate is provided with two or more open slots, open slot
It is communicated with inside cavity, the under shed for connection electrode slot and as electrophoresis conduction mouth, open slot is close equipped with what be can be removed
Strip of paper used for sealing.
3. a kind of gel for electrophoresis preparation facilities according to claim 2, it is characterised in that: between upper offset plate and lower offset plate
It is fixed by ultrasonic bonding.
4. a kind of gel for electrophoresis preparation facilities according to claim 3, it is characterised in that: upper offset plate or lower offset plate two sides
Equipped with several limited posts, for defining the thickness of ultrasonic bonding.
5. a kind of gel for electrophoresis preparation facilities according to claim 1, it is characterised in that: each on the two sides of upper offset plate
If the open end of two "u"-shapeds is oppositely arranged there are two "u"-shaped, accordingly set that there are two rectangles on lower offset plate
Raised A, raised A buckle into two "u"-shapeds formation gap in.
6. a kind of gel for electrophoresis preparation facilities according to claim 1, it is characterised in that: opposite with liquid leakage tank on comb body
One side, be provided along its length the raised B an of rectangular shape, the thickness of raised B offset plate thickness one adjacent thereto
It causes.
7. a kind of gel for electrophoresis preparation facilities according to claim 1, it is characterised in that: with liquid leakage tank in the same side
The inside of offset plate is provided along its length several grooves, and vertical height of the groove on offset plate, which is greater than to pour into cavity, coagulates
The vertical height of glue, lower than the vertical height of comb teeth top end in insertion cavity.
8. a kind of gel for electrophoresis preparation facilities according to claim 7, it is characterised in that: the shape of groove can be circle
Shape, diamond shape, square or rectangular.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910592660.6A CN110215884A (en) | 2019-07-03 | 2019-07-03 | A kind of gel for electrophoresis preparation facilities |
| CN202010178135.2A CN111558347A (en) | 2019-07-03 | 2020-03-14 | Gel preparation facilities for electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910592660.6A CN110215884A (en) | 2019-07-03 | 2019-07-03 | A kind of gel for electrophoresis preparation facilities |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110215884A true CN110215884A (en) | 2019-09-10 |
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910592660.6A Pending CN110215884A (en) | 2019-07-03 | 2019-07-03 | A kind of gel for electrophoresis preparation facilities |
| CN202010178135.2A Pending CN111558347A (en) | 2019-07-03 | 2020-03-14 | Gel preparation facilities for electrophoresis |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010178135.2A Pending CN111558347A (en) | 2019-07-03 | 2020-03-14 | Gel preparation facilities for electrophoresis |
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| CN (2) | CN110215884A (en) |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5284565A (en) * | 1992-12-23 | 1994-02-08 | Bio-Rad Laboratories, Inc. | Sample well insert with wedge-shaped profile for ultra-thin slab gels in electrophoresis |
| JP3541971B2 (en) * | 1994-12-13 | 2004-07-14 | 憲史 弘田 | Analysis method using electrophoresis device |
| US6379519B1 (en) * | 1999-09-01 | 2002-04-30 | Mirador Dna Design Inc. | Disposable thermoformed electrophoresis cassette |
| WO2013180639A1 (en) * | 2012-05-31 | 2013-12-05 | Ge Healthcare Bio-Sciences Ab | An electrophoresis tray and a method of running an electrophoresis experiment |
| US9061236B2 (en) * | 2012-11-20 | 2015-06-23 | Nanjingjinsirui Science & Technology Biology Corporation | Gel electrophoresis device for loading large sample volumes |
| ES2829617T3 (en) * | 2013-08-09 | 2021-06-01 | Helena Laboratories Corp | Applicator comb for gel electrophoresis |
| CN203849203U (en) * | 2014-05-27 | 2014-09-24 | 李水清 | Sampler for agarose gel electrophoresis |
| CN107128864B (en) * | 2017-03-21 | 2019-02-05 | 上海万生昊天生物技术有限公司 | The Automatic Control glue pouring system and method continuously made for gradient gel film |
| CN210332608U (en) * | 2019-07-03 | 2020-04-17 | 常州天地人和生物科技有限公司 | Gel preparation facilities for electrophoresis |
-
2019
- 2019-07-03 CN CN201910592660.6A patent/CN110215884A/en active Pending
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2020
- 2020-03-14 CN CN202010178135.2A patent/CN111558347A/en active Pending
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| CN111558347A (en) | 2020-08-21 |
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Application publication date: 20190910 |
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