CN117402725A - Automatic control system of high-pressure cell culture cabin - Google Patents
Automatic control system of high-pressure cell culture cabin Download PDFInfo
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- CN117402725A CN117402725A CN202311343978.3A CN202311343978A CN117402725A CN 117402725 A CN117402725 A CN 117402725A CN 202311343978 A CN202311343978 A CN 202311343978A CN 117402725 A CN117402725 A CN 117402725A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 28
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 130
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 65
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 65
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 64
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 64
- 239000001301 oxygen Substances 0.000 claims abstract description 64
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 165
- 239000003507 refrigerant Substances 0.000 claims description 89
- 230000001502 supplementing effect Effects 0.000 claims description 46
- 239000007789 gas Substances 0.000 claims description 44
- 238000005070 sampling Methods 0.000 claims description 26
- 238000005057 refrigeration Methods 0.000 claims description 19
- 239000010865 sewage Substances 0.000 claims description 15
- 239000003638 chemical reducing agent Substances 0.000 claims description 14
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 6
- 239000013589 supplement Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 4
- 238000007599 discharging Methods 0.000 claims description 2
- 238000004378 air conditioning Methods 0.000 claims 1
- 238000009529 body temperature measurement Methods 0.000 claims 1
- 239000008399 tap water Substances 0.000 claims 1
- 235000020679 tap water Nutrition 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 10
- 238000010438 heat treatment Methods 0.000 abstract description 7
- 108010066057 cabin-1 Proteins 0.000 description 20
- 230000003020 moisturizing effect Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M37/04—Seals
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
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Abstract
The invention discloses an automatic control system of a high-pressure cell culture cabin, which is provided with a cabin internal pressure control structure and a control process thereof, so that the internal pressure of the cabin is always kept at a pressure value corresponding to the depth of a target simulated underwater; the system is provided with a control structure and a control process of oxygen concentration and carbon dioxide concentration in the cabin, oxygen is supplemented when the oxygen concentration value in the cabin is low, and carbon dioxide is supplemented when the carbon dioxide concentration value in the cabin is low, so that the oxygen concentration value and the carbon dioxide concentration value in the cabin are respectively kept at a set oxygen concentration value and a set carbon dioxide concentration value all the time; the system is provided with a cabin internal temperature control structure and a control process thereof, heating is carried out when the temperature in the cabin is low, and refrigerating is carried out when the temperature in the cabin is high, so that the temperature in the cabin is always kept in a set temperature range.
Description
Technical Field
The invention relates to the technical field of high-pressure cell culture cabins, in particular to an automatic control system of a high-pressure cell culture cabin.
Background
The high-pressure cell culture cabin is jointly designed before the unit and university, and the patent with the application number of 2018115822134 is filed, and the patent can realize the partial pressure control of the carbon dioxide in the cabin under different pressures by controlling the concentration of the carbon dioxide in a high-pressure air source, and does not consider whether the condition of the oxygen concentration in the cabin is suitable for cell culture or not; the patent heats the temperature in the cabin, and does not consider the situation that the temperature in the cabin is higher and needs to be reduced.
Disclosure of Invention
The invention provides an automatic control system of a high-pressure cell culture cabin, aiming at the problems and the defects existing in the prior art.
The invention solves the technical problems by the following technical proposal:
the invention provides an automatic control system of a high-pressure cell culture cabin, which comprises a cabin body and a cabin door, wherein a cell culture dish is placed in the cabin body, and the automatic control system is characterized by comprising the following components: the cabin body is fixedly communicated with one end of a pressurizing pipeline, the other end of the pressurizing pipeline is externally connected with a mixed gas source, a pressurizing electric valve is arranged on the pressurizing pipeline and positioned outside the cabin body, the cabin body is fixedly communicated with one end of a pressure reducing pipeline, the other end of the pressure reducing pipeline is arranged outside the cabin body, the pressure reducing pipeline is provided with a pressure reducing electric valve positioned outside the cabin body, the cabin body is fixedly communicated with one end of an oxygen supplementing pipeline, the other end of the oxygen supplementing pipeline is externally connected with a pure oxygen source, an oxygen supplementing electric valve is arranged on the oxygen supplementing pipeline and located outside the cabin body, one end of the carbon dioxide supplementing pipeline is fixedly communicated with the cabin body, the other end of the carbon dioxide supplementing pipeline is externally connected with a pure carbon dioxide source, and the carbon dioxide supplementing electric valve is arranged on the carbon dioxide supplementing pipeline and located outside the cabin body.
The cabin body is internally provided with a water inlet, a water outlet and a water outlet, wherein the water inlet of the water inlet is fixedly provided with a hot water coil, a refrigerant coil and a fan, the water inlet of the hot water coil penetrates through the cabin body and is communicated with the water outlet of a circulating water pump through a second circulating hot water inlet pipe, the water inlet of the circulating water pump is communicated with the water outlet of a water tank through a first circulating hot water inlet pipe, an electric heater is arranged in the water tank, the second circulating hot water inlet pipe is provided with a hot water inlet electric valve, the water return port of the hot water coil penetrates through the cabin body and is communicated with the water return port of the water tank through a circulating water return pipe, the circulating water return pipe is provided with a water return electric valve, the system refrigerant port of the refrigerant coil penetrates through the cabin body and is communicated with the outlet of a circulating refrigerant pump through a second circulating refrigerant inlet pipe, the inlet of the circulating refrigerant pump is communicated with the outlet of a refrigerating equipment through a first circulating refrigerant inlet pipe, the second circulating refrigerant inlet electric valve is arranged on the circulating refrigerant inlet pipe, the refrigerant return port of the refrigerant coil penetrates through the cabin body and is communicated with the return port of the equipment through a circulating refrigerant return pipe, and the circulating electric valve is provided with the refrigerating body 54, and the temperature sensor is in the existing market.
The pressure measuring device is characterized in that one end of a pressure measuring pipeline is fixedly communicated with the cabin body, the other end of the pressure measuring pipeline is sealed and penetrates through the cabin body, a pressure measuring electric valve and a pressure sensor are arranged on the pressure measuring pipeline and outside the cabin body, one end of a sampling pipeline is fixedly communicated with the cabin body, the other end of the sampling pipeline penetrates through the cabin body and then is connected and communicated with the gas analyzer through a pressure reducer, and a sampling electric valve is arranged on the sampling pipeline and outside the cabin body.
The controller is used for controlling the pressure sensor to open and the pressure measuring electric valve to open, receiving the pressure value in the cabin detected by the pressure sensor, judging the relation between the pressure value in the cabin and the pressure value corresponding to the target simulated underwater depth, controlling the pressure measuring electric valve to open when the pressure value in the cabin is lower than the pressure value corresponding to the target simulated underwater depth, pressurizing the cabin by the mixed gas in the mixed gas source through the pressurizing pipeline until the pressure value in the cabin reaches the pressure value corresponding to the target simulated underwater depth, closing the pressure measuring electric valve, controlling the pressure reducing electric valve to open when the pressure value in the cabin is higher than the pressure value corresponding to the target simulated underwater depth, discharging the mixed gas in the cabin through the pressure reducing pipeline until the pressure value in the cabin reaches the pressure value corresponding to the target simulated underwater depth, and closing the pressure reducing electric valve.
The controller is used for controlling the gas analyzer to be opened, the sampling electric valve to be opened, the gas in the cabin body flows through the sampling pipeline and flows into the gas analyzer after being depressurized by the pressure reducer, the gas analyzer is used for analyzing the oxygen concentration value and the carbon dioxide concentration value in the gas in the cabin body, the controller is used for judging whether the oxygen concentration value is lower than the set oxygen concentration value, when the oxygen concentration value is lower than the set oxygen concentration value, the controller is used for controlling the oxygen supplementing electric valve to be opened, the oxygen in the pure oxygen source supplements oxygen in the cabin body until the oxygen concentration value in the cabin body reaches the set oxygen concentration value through the oxygen supplementing pipeline, the controller is used for judging whether the carbon dioxide concentration value is lower than the set carbon dioxide concentration value, when the carbon dioxide concentration value is lower than the set carbon dioxide concentration value, the carbon dioxide in the pure carbon dioxide source supplements the cabin body through the carbon dioxide supplementing pipeline until the carbon dioxide concentration value in the cabin body reaches the set carbon dioxide concentration value.
The temperature sensor is used for detecting the temperature in the cabin body and transmitting the temperature value to the controller.
The controller is used for judging whether the temperature value is lower than the lower limit value of the set temperature range, if yes, the electric heater is controlled to be started, the electric heater is controlled to heat water in the water tank, the circulating water pump is controlled to be started after the set time is heated, the hot water inlet electric valve and the backwater electric valve are opened, the circulating water pump pumps hot water in the water tank, the water in the water tank flows into the hot water coil pipe to exchange heat with air in the cabin body after sequentially flowing through the first circulating hot water inlet pipe, the circulating water pump and the second circulating hot water inlet pipe, the water after heat exchange flows into the water tank through the circulating backwater pipe, the temperature in the cabin body rises until the temperature value in the cabin body reaches the target temperature value in the set temperature range, the electric heater and the circulating water pump are stopped to work, and the hot water inlet electric valve and the backwater electric valve are closed.
The controller is used for judging whether the temperature value is higher than the upper limit value of the set temperature range, if yes, the refrigeration equipment is controlled to be started, the circulating refrigerant pump is controlled to be started, the system refrigerant electric valve and the back refrigerant electric valve are controlled to be started after the set refrigeration time, the circulating refrigerant pump pumps the refrigerant in the refrigeration equipment, the refrigerant in the refrigeration equipment sequentially flows through the first circulating refrigerant inlet pipe, the circulating refrigerant pump and the second circulating refrigerant inlet pipe and then flows into the refrigerant coil to exchange heat with air in the cabin body, the refrigerant after heat exchange flows into the refrigeration equipment through the back refrigerant pipe, the temperature in the cabin body is reduced until the temperature value in the cabin body reaches the target temperature value in the set temperature range, and the refrigeration equipment and the circulating refrigerant pump are suspended to work, and the refrigerant inlet electric valve and the back refrigerant electric valve are closed.
The invention has the positive progress effects that:
compared with the prior patent, the invention is provided with the cabin internal pressure control structure and the control process thereof, so that the pressure in the cabin is always kept at the pressure value corresponding to the target simulated underwater depth.
Compared with the prior patent application, the invention considers not only the control of the carbon dioxide concentration, but also the control of the oxygen concentration, and is provided with a structure and a control process for controlling the oxygen concentration and the carbon dioxide concentration in the cabin, wherein oxygen is supplemented when the oxygen concentration value in the cabin is low, and carbon dioxide is supplemented when the carbon dioxide concentration value in the cabin is low, so that the oxygen concentration value and the carbon dioxide concentration value in the cabin are respectively kept at the set oxygen concentration value and the set carbon dioxide concentration value all the time.
Compared with the prior patent, the invention considers the condition of low temperature in the cabin and the condition of high temperature in the cabin, is provided with the cabin temperature control structure and the control process thereof, and heats up when the temperature in the cabin is low, and cools down when the temperature in the cabin is high, so that the temperature in the cabin is always kept in a set temperature range, and the heating mode (water heating) of the invention is different from the heating mode (gas heating) of the prior patent.
Drawings
FIG. 1 is a schematic diagram showing the construction of an automatic control system for a high-pressure cell culture chamber according to a preferred embodiment of the present invention.
Fig. 2 is a control schematic diagram of heating temperature control according to a preferred embodiment of the present invention.
Fig. 3 is a control schematic diagram of the refrigeration temperature control according to the preferred embodiment of the present invention.
FIG. 4 is a control schematic diagram of pressure control, oxygen concentration and carbon dioxide concentration control according to a preferred embodiment of the present invention.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1-4, the embodiment provides an automatic control system for a high-pressure cell culture cabin, wherein the high-pressure cell culture cabin comprises a cabin body 1 and a cabin door 2, and a cell culture dish is placed in the cabin body 1. The high-pressure cell culture cabin is an experimental instrument for bacteria and cell culture, and the principle is that an artificial environment for the growth and propagation of microorganisms, cells and bacteria is formed in the culture cabin, such as controlling certain temperature, pressure and the like.
The system comprises: the cabin body 1 is fixedly communicated with one end of a pressurizing pipeline 3, the other end of the pressurizing pipeline 3 is externally connected with a mixed gas source 4, the parts of the pressurizing pipeline 3, which are respectively positioned inside and outside the cabin body 1, are connected and communicated by adopting cabin through pipe fittings, and a pressurizing electric valve 5 is arranged on the pressurizing pipeline 3 and positioned outside the cabin body 1; the cabin body 1 is fixedly communicated with one end of a pressure reducing pipeline 6, the other end of the pressure reducing pipeline 6 is arranged outside the cabin body 1, the parts of the pressure reducing pipeline 6, which are respectively positioned inside and outside the cabin body 1, are connected and communicated by adopting cabin through pipe fittings, and a pressure reducing electric valve 7 is arranged on the pressure reducing pipeline 6 and positioned outside the cabin body 1; the cabin body 1 is fixedly communicated with one end of an oxygen supplementing pipeline 8, the other end of the oxygen supplementing pipeline 8 is externally connected with a pure oxygen source 9, the parts of the oxygen supplementing pipeline 8, which are respectively positioned inside and outside the cabin body 1, are connected and communicated by adopting cabin through pipe fittings, and an oxygen supplementing electric valve 10 is arranged on the oxygen supplementing pipeline 8 and positioned outside the cabin body 1; the cabin body 1 is fixedly communicated with one end of a carbon dioxide supplementing pipeline 11, the other end of the carbon dioxide supplementing pipeline 11 is externally connected with a pure carbon dioxide gas source 12, the parts of the carbon dioxide supplementing pipeline 11, which are respectively positioned inside and outside the cabin body 1, are connected and communicated by adopting cabin communicating pipes, and a carbon dioxide supplementing electric valve 13 is arranged on the carbon dioxide supplementing pipeline 11 and positioned outside the cabin body 1.
The end of the cabin body 1 far away from the cabin door 2 is fixedly provided with a hot water coil 14, a refrigerant coil 15 and a fan 16, the fan 16 is positioned above the hot water coil 14 and the refrigerant coil 15, and the hot water coil 14 and the refrigerant coil 15 are arranged in a staggered manner, such as the end of the hot water coil 14 which is closer to the cabin body 1 and far away from the cabin door 2 relative to the refrigerant coil 15.
The water inlet of the hot water coil pipe 14 penetrates through the cabin body 1 and is connected and communicated with the water outlet of the circulating water pump 18 through the second circulating water inlet pipe 17, the water inlet of the circulating water pump 18 is connected and communicated with the water outlet of the water tank 20 through the first circulating water inlet pipe 19, the electric heater 21 is arranged in the water tank 20, the second circulating water inlet pipe 17 is provided with the hot water inlet electric valve 22, the water return port of the hot water coil pipe 14 penetrates through the cabin body 1 and is connected and communicated with the water return port of the water tank 20 through the circulating water return pipe 23, and the circulating water return pipe 23 is provided with the water return electric valve 24.
And, be equipped with moisturizing case 25 directly over the water tank 20, connect through moisturizing pipeline 26 between moisturizing case 25 and the water tank 20 and communicate with each other, be equipped with moisturizing motorised valve 27 on the moisturizing pipeline 26, be equipped with level sensor 28 in the water tank 20, the upper portion of moisturizing case 25 is equipped with the water supply socket 29 of external running water.
The cabin body 1 is internally provided with a temperature sensor 30, and the temperature sensor 30 is electrically connected with a controller 31.
The binary refrigerant port of the refrigerant coil 15 penetrates through the cabin 1 and is communicated with the outlet of the circulating refrigerant pump 33 through the second circulating refrigerant inlet pipe 32, the inlet of the circulating refrigerant pump 33 is communicated with the outlet of the refrigerating equipment 35 (existing equipment) through the first circulating refrigerant inlet pipe 34, the second circulating refrigerant inlet pipe 32 is provided with a refrigerant inlet electric valve 36, the refrigerant return port of the refrigerant coil 15 penetrates through the cabin 1 and is communicated with the return port of the refrigerating equipment 35 through the circulating refrigerant return pipe 37, and the circulating refrigerant return pipe 37 is provided with a refrigerant return electric valve 38.
The cabin body 1 is fixedly communicated with one end of a pressure measuring pipeline 39, the other end of the pressure measuring pipeline 39 is sealed and penetrates through the cabin body 1, the parts of the pressure measuring pipeline 39, which are respectively positioned inside and outside the cabin body 1, are connected and communicated through cabin through pipe fittings, a pressure measuring electric valve 40, a pressure sensor 41 and a pressure gauge 42 are arranged on the pressure measuring pipeline 39, and the pressure measuring electric valve 40, the pressure sensor 41 and the pressure gauge 42 are arranged outside the cabin body 1. The pressure gauge 42 is a Y-150B type precision pressure gauge, the measuring range is 0-4.0MPa, the precision level is 0.4, the pressure gauge 42 is used for displaying the real-time pressure value in the cabin body 1, and the pressure gauge 42 is placed at a position convenient to observe.
The cabin body 1 is fixedly communicated with one end of a sampling pipeline 43, the other end of the sampling pipeline 43 penetrates through the cabin body 1 and is communicated with a gas analyzer 46 through a pressure reducer 44 (YQY-9) and a flowmeter 45, the parts of the sampling pipeline 43, which are respectively positioned inside and outside the cabin body 1, are communicated through cabin pipe fittings, a sampling electric valve 47 is arranged on the sampling pipeline 43, and the pressure reducer 44, the flowmeter 45, the gas analyzer 46 and the sampling electric valve 47 are arranged outside the cabin body 1; the pressure reducer 44 is also in line connection with an air source 48, which is provided with a calibrated electrically operated valve 49. Wherein the range of the flowmeter 45 is 40-400ml/min, and is used for detecting the gas flow of the sampling pipeline after the pressure is reduced by the pressure reducer 44. The gas analyzer 46 may be an MSA type digital display gas analyzer, and is configured to detect the oxygen concentration and the carbon dioxide concentration of the environmental gas in the cabin, and when the oxygen concentration or the carbon dioxide concentration of the measured medium reaches a set value, an audible and visual alarm signal is displayed.
The following specifically describes the automatic control process of the automatic control system of the high-pressure cell culture cabin:
the pressure control process in the cabin body comprises the following steps: the controller 31 is configured to control the pressure sensor 41 to open and the pressure measuring electric valve 40 to open, receive the pressure value in the cabin 1 detected by the pressure sensor 41, determine a relationship between the pressure value in the cabin 1 and the pressure value corresponding to the target simulated underwater depth, control the pressure measuring electric valve 5 to open when the pressure value in the cabin 1 is lower than the pressure value corresponding to the target simulated underwater depth, pressurize the cabin 1 by the mixed gas in the mixed gas source 4 through the pressurizing pipeline 3 until the pressure value in the cabin 1 reaches the pressure value corresponding to the target simulated underwater depth, close the pressure measuring electric valve 5, control the pressure reducing electric valve 7 to open when the pressure value in the cabin 1 is higher than the pressure value corresponding to the target simulated underwater depth, and discharge the mixed gas in the cabin 1 through the pressure reducing pipeline 6 until the pressure value in the cabin 1 reaches the pressure value corresponding to the target simulated underwater depth, and close the pressure reducing electric valve 7. Therefore, the pressure in the cabin body is controlled, and the pressure in the cabin body 1 is always kept at a pressure value corresponding to the depth of the target simulated underwater.
And controlling the oxygen concentration and the carbon dioxide concentration in the cabin: the gas analyzer 46 is calibrated before use, the controller 31 is used for controlling the opening of the calibration motor valve 49, and standard air in the air source 48 enters the gas analyzer 46 after passing through the calibration motor valve 49, the pressure reducer 44 and the flow meter 45, and the gas analyzer 46 is calibrated by using the standard air. When the intelligent control system is used, the controller 31 is used for controlling the gas analyzer 46 to be opened, the sampling electric valve 47 to be opened, the gas in the cabin body 1 flows into the gas analyzer 46 after flowing through the sampling pipeline 43 and being depressurized through the depressurizer 44 and the flowmeter 45, the gas analyzer 46 is used for analyzing the oxygen concentration value and the carbon dioxide concentration value in the gas in the cabin body 1, the controller 31 is used for judging whether the oxygen concentration value is lower than the set oxygen concentration value, when yes, the controller 31 is used for controlling the oxygen supplementing electric valve 10 to be opened, the oxygen in the pure oxygen source 9 supplements oxygen in the cabin body 1 through the oxygen supplementing pipeline 8 until the oxygen concentration value in the cabin body 1 reaches the set oxygen concentration value, and when yes, the controller 31 is used for judging whether the carbon dioxide concentration value is lower than the set carbon dioxide concentration value, and when yes, the carbon dioxide in the pure carbon dioxide source 12 supplements carbon dioxide in the cabin body 1 through the carbon dioxide supplementing pipeline 11 until the carbon dioxide concentration value in the cabin body 1 reaches the set carbon dioxide concentration value. Thereby realizing the control of the oxygen concentration and the carbon dioxide concentration in the cabin body 1, and enabling the oxygen concentration value and the carbon dioxide concentration value in the cabin body 1 to be respectively kept at the set oxygen concentration value and the set carbon dioxide concentration value all the time.
The temperature control process in the cabin body comprises the following steps: the temperature sensor 30 is used to detect the temperature inside the cabin 1 and to transmit the temperature value to the controller 31. The controller 31 is configured to determine whether the temperature value is lower than the lower limit value of the set temperature range, if yes, control the electric heater 21 to be turned on, heat the water in the water tank 20 by the electric heater 21, after heating for a set time, control the circulation water pump 18 to be turned on, the hot water inlet electric valve 22 and the return water electric valve 24 to be turned on, the circulation water pump 18 pumps the hot water in the water tank 20, so that the water in the water tank 20 flows through the first circulation hot water inlet pipe 19, the circulation water pump 18 and the second circulation hot water inlet pipe 17 in sequence, and then flows into the hot water coil 14 to exchange heat with the air in the cabin 1, the water after heat exchange flows into the water tank 20 through the circulation return water pipe 23, and the temperature in the cabin 1 rises until the temperature value in the cabin 1 reaches the target temperature value in the set temperature range, and the operation of the electric heater 21 and the circulation water pump 18 is suspended, and the hot water inlet electric valve 22 and the return water electric valve 24 are closed. The liquid level sensor 28 is used for detecting the water level in the water tank 20 and transmitting the water level value to the controller 31, and the controller 31 is used for judging whether the water level value is lower than the set water level value, and if yes, the water replenishing valve 27 is opened so that the water in the water replenishing tank 26 flows into the water tank 20 through the water replenishing pipeline 26.
The controller 31 is configured to determine whether the temperature value is higher than the upper limit value of the set temperature range, if yes, control the refrigeration device 35 to be turned on, control the circulation refrigerant pump 33 to be turned on, the circulation refrigerant motor-operated valve 36 and the return refrigerant motor-operated valve 38 to be turned on after the set time of refrigeration, pump the refrigerant in the refrigeration device 35 by the circulation refrigerant pump 33, so that the refrigerant in the refrigeration device 35 flows through the first circulation refrigerant inlet pipe 34, the circulation refrigerant pump 33 and the second circulation refrigerant inlet pipe 32 in sequence, flows into the refrigerant coil 15 to exchange heat with the air in the cabin 1, the refrigerant after heat exchange flows into the refrigeration device 35 through the circulation refrigerant return pipe 37, and the temperature in the cabin 1 is reduced until the temperature value in the cabin 1 reaches the target temperature value in the set temperature range, and the refrigeration device 35 and the circulation refrigerant pump 33 are suspended to operate, and the refrigerant motor-operated valve 36 and the return refrigerant motor-operated valve 38 are turned off. Thereby realizing the temperature control in the cabin 1, so that the temperature in the cabin 1 is always kept in the set temperature range.
In the above control process, the controller 31 controls the blower 16 to be turned on so that the temperature distribution in the cabin 1 is uniform.
In addition, the bottom of the cabin body 1 is fixedly communicated with one end of a sewage pipeline, the other end of the sewage pipeline is arranged outside the cabin body, the parts of the sewage pipeline, which are respectively positioned inside and outside the cabin body 1, are connected and communicated by adopting a cabin through pipe fitting, the sewage pipeline is provided with a sewage electric valve, and the controller is used for controlling the sewage electric valve to be opened, so that sewage in the cabin body is discharged through the sewage pipeline.
The temperature measuring pipeline between the temperature sensor 30 and the controller 31 can be provided with a temperature display instrument, the temperature display instrument is arranged outside the cabin body 1, and the temperature display instrument is used for displaying real-time temperature values in the cabin body 1. The temperature display instrument can adopt a WMNK-404 digital display temperature control instrument which is specially used for displaying the temperature in a pressure environment, wherein the temperature range is 0-100 ℃, the precision is +/-0.2 ℃, and the temperature display instrument is placed at a position convenient to observe.
While specific embodiments of the invention have been described above, it will be appreciated by those skilled in the art that these are by way of example only, and the scope of the invention is defined by the appended claims. Various changes and modifications to these embodiments may be made by those skilled in the art without departing from the principles and spirit of the invention, but such changes and modifications fall within the scope of the invention.
Claims (9)
1. An automatic control system for a high-pressure cell culture chamber, the high-pressure cell culture chamber comprising a chamber body and a chamber door, wherein a cell culture dish is placed in the chamber body, the system comprising: the pressure-reducing type air-conditioning system is characterized in that one end of a pressurizing pipeline is fixedly communicated with the cabin body, the other end of the pressurizing pipeline is externally connected with a mixed air source, a pressurizing electric valve is arranged on the pressurizing pipeline and positioned outside the cabin body, one end of a pressure-reducing pipeline is fixedly communicated with the cabin body, the other end of the pressure-reducing pipeline is arranged outside the cabin body, a pressure-reducing electric valve is arranged on the pressure-reducing pipeline and positioned outside the cabin body, one end of an oxygen supplementing pipeline is fixedly communicated with the cabin body, the other end of the oxygen supplementing pipeline is externally connected with a pure oxygen source, an oxygen supplementing electric valve is arranged on the oxygen supplementing pipeline and positioned outside the cabin body, one end of a carbon dioxide supplementing pipeline is fixedly communicated with the cabin body, the other end of the carbon dioxide supplementing pipeline is externally connected with a pure carbon dioxide source, and a carbon dioxide supplementing electric valve is arranged on the carbon dioxide supplementing pipeline and positioned outside the cabin body;
a hot water coil, a refrigerant coil and a fan are fixed at the end, far away from the cabin door, of the cabin body, a hot water inlet of the hot water coil penetrates through the cabin body and is communicated with a water outlet of a circulating water pump through a second circulating hot water inlet pipe, a water inlet of the circulating water pump is communicated with a water outlet of a water tank through a first circulating hot water inlet pipe, an electric heater is arranged in the water tank, a hot water inlet electric valve is arranged on the second circulating hot water inlet pipe, a water return opening of the hot water coil penetrates through the cabin body and is communicated with a water return opening of the water tank through a circulating water return pipe, a water return electric valve is arranged on the circulating water return pipe, a binary refrigerant opening of the refrigerant coil penetrates through the cabin body and is communicated with an outlet of a circulating refrigerant pump through a second circulating refrigerant inlet pipe, an inlet of the circulating refrigerant pump is communicated with an outlet of a refrigerating device through a first circulating refrigerant inlet pipe, a refrigerant electric valve is arranged on the second circulating refrigerant inlet pipe, a refrigerant return opening of the refrigerant coil penetrates through the cabin body and is communicated with a return opening of the device through a circulating refrigerant return pipe, a temperature sensor is arranged on the refrigerant electric return valve, and the temperature sensor is connected with the cabin body;
the pressure measuring device comprises a cabin body, a pressure measuring pipeline, a pressure measuring electric valve, a pressure sensor, a sampling pipeline, a pressure reducer, a gas analyzer, a sampling pipeline and a sampling pipeline, wherein the cabin body is fixedly communicated with one end of the pressure measuring pipeline, the other end of the pressure measuring pipeline is sealed and penetrates through the cabin body, the pressure measuring pipeline is provided with the pressure measuring electric valve and the pressure sensor outside the cabin body, the cabin body is fixedly communicated with one end of the sampling pipeline, the other end of the sampling pipeline penetrates through the cabin body and is connected and communicated with the gas analyzer through the pressure reducer, and the sampling pipeline is provided with the sampling electric valve outside the cabin body;
the controller is used for controlling the pressure sensor to open and the pressure measuring electric valve to open, receiving the pressure value in the cabin detected by the pressure sensor, judging the relation between the pressure value in the cabin and the pressure value corresponding to the target simulated underwater depth, controlling the pressure measuring electric valve to open when the pressure value in the cabin is lower than the pressure value corresponding to the target simulated underwater depth, pressurizing the cabin by the mixed gas in the mixed gas source through the pressurizing pipeline until the pressure value in the cabin reaches the pressure value corresponding to the target simulated underwater depth, closing the pressure measuring electric valve, controlling the pressure reducing electric valve to open when the pressure value in the cabin is higher than the pressure value corresponding to the target simulated underwater depth, discharging the mixed gas in the cabin through the pressure reducing pipeline until the pressure value in the cabin reaches the pressure value corresponding to the target simulated underwater depth, and closing the pressure reducing electric valve;
the controller is used for controlling the gas analyzer to be opened, the sampling electric valve to be opened, the gas in the cabin body flows through the sampling pipeline and flows into the gas analyzer after being depressurized by the pressure reducer, the gas analyzer is used for analyzing the oxygen concentration value and the carbon dioxide concentration value in the gas in the cabin body, the controller is used for judging whether the oxygen concentration value is lower than the set oxygen concentration value, when the oxygen concentration value is lower than the set oxygen concentration value, the controller is used for controlling the oxygen supplementing electric valve to be opened, the oxygen in the pure oxygen source supplements oxygen in the cabin body through the oxygen supplementing pipeline until the oxygen concentration value in the cabin body reaches the set oxygen concentration value, the controller is used for judging whether the carbon dioxide concentration value is lower than the set carbon dioxide concentration value, when the carbon dioxide concentration value is lower than the set carbon dioxide concentration value, the carbon dioxide in the pure carbon dioxide source supplements the cabin body through the carbon dioxide supplementing pipeline until the carbon dioxide concentration value in the cabin body reaches the set carbon dioxide concentration value;
the temperature sensor is used for detecting the temperature in the cabin body and transmitting the temperature value to the controller;
the controller is used for judging whether the temperature value is lower than the lower limit value of the set temperature range, if yes, the electric heater is controlled to be started, the electric heater is controlled to heat water in the water tank, the circulating water pump is controlled to be started after the set time is heated, the hot water inlet electric valve and the return water electric valve are opened, the circulating water pump pumps hot water in the water tank, so that the water in the water tank flows into the hot water coil pipe to exchange heat with air in the cabin body after sequentially flowing through the first circulating hot water inlet pipe, the circulating water pump and the second circulating hot water inlet pipe, the water after heat exchange flows into the water tank through the circulating return pipe, the temperature in the cabin body is increased until the temperature value in the cabin body reaches the target temperature value in the set temperature range, the electric heater and the circulating water pump are stopped, and the hot water inlet electric valve and the return water electric valve are closed;
the controller is used for judging whether the temperature value is higher than the upper limit value of the set temperature range, if yes, the refrigeration equipment is controlled to be started, the circulating refrigerant pump is controlled to be started, the system refrigerant electric valve and the back refrigerant electric valve are controlled to be started after the set refrigeration time, the circulating refrigerant pump pumps the refrigerant in the refrigeration equipment, the refrigerant in the refrigeration equipment sequentially flows through the first circulating refrigerant inlet pipe, the circulating refrigerant pump and the second circulating refrigerant inlet pipe and then flows into the refrigerant coil to exchange heat with air in the cabin body, the refrigerant after heat exchange flows into the refrigeration equipment through the back refrigerant pipe, the temperature in the cabin body is reduced until the temperature value in the cabin body reaches the target temperature value in the set temperature range, and the refrigeration equipment and the circulating refrigerant pump are suspended to work, and the refrigerant inlet electric valve and the back refrigerant electric valve are closed.
2. The automated high pressure cell culture chamber control system of claim 1, wherein a flow meter is disposed on the line between the pressure reducer and the gas analyzer, the flow meter being configured to detect the flow of gas after the sampling line is depressurized by the pressure reducer.
3. The automatic control system of the high-pressure cell culture cabin according to claim 1, wherein the pressure reducer is connected with an air source through a pipeline, a calibration electric valve is arranged on the pipeline, the controller is used for controlling the calibration electric valve to be opened, standard air in the air source enters the gas analyzer after passing through the calibration electric valve and the pressure reducer, and the gas analyzer is calibrated by the standard air.
4. The automatic control system of the high-pressure cell culture cabin according to claim 1, further comprising a water supplementing tank, wherein the water supplementing tank is positioned right above the water tank, the water supplementing tank is communicated with the water tank through a water supplementing pipeline, a water supplementing electric valve is arranged on the water supplementing pipeline, a liquid level sensor is arranged in the water tank, and a water supply socket externally connected with tap water is arranged at the upper part of the water supplementing tank;
the liquid level sensor is used for detecting the water level in the water tank and transmitting the water level value to the controller, the controller is used for judging whether the water level value is lower than a set liquid level value, and if yes, the water supplementing electric valve is opened, so that water in the water supplementing tank flows into the water tank through the water supplementing pipeline.
5. The automated high pressure cell culture chamber control system of claim 1, wherein the fan is positioned above the hot water coil and the refrigerant coil, the hot water coil and the refrigerant coil being offset.
6. The automatic control system of the high-pressure cell culture cabin according to claim 1, wherein the bottom of the cabin body is fixedly communicated with one end of a sewage pipeline, the other end of the sewage pipeline is arranged outside the cabin body, a sewage electric valve is arranged on the sewage pipeline, and the controller is used for controlling the opening of the sewage electric valve, and sewage in the cabin body is discharged through the sewage pipeline.
7. The automatic control system of the high-pressure cell culture chamber according to claim 6, wherein the parts of the pressurizing pipeline, which are respectively positioned inside and outside the chamber body, are connected and communicated by adopting a chamber through pipe, the parts of the depressurizing pipeline, which are respectively positioned inside and outside the chamber body, are connected and communicated by adopting a chamber through pipe, the parts of the oxygen supplementing pipeline, which are respectively positioned inside and outside the chamber body, are connected and communicated by adopting a chamber through pipe, the parts of the pressure measuring pipeline, which are respectively positioned inside and outside the chamber body, are connected and communicated by adopting a chamber through pipe, the parts of the sampling pipeline, which are respectively positioned inside and outside the chamber body, are connected and communicated by adopting a chamber through pipe, and the parts of the blowdown pipeline, which are respectively positioned inside and outside the chamber body, are connected and communicated by adopting a chamber through pipe.
8. The automated high pressure cell culture chamber control system of claim 1, further comprising a temperature display disposed on the temperature measurement line between the temperature sensor and the controller, the temperature display being disposed outside the chamber and configured to display real-time temperature values within the chamber.
9. The automated high pressure cell culture chamber control system of claim 1, further comprising a pressure gauge disposed on the pressure line, the pressure gauge disposed outside the chamber, the pressure gauge configured to display real-time pressure values within the chamber.
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| CN202311343978.3A CN117402725A (en) | 2023-10-17 | 2023-10-17 | Automatic control system of high-pressure cell culture cabin |
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| CN202311343978.3A CN117402725A (en) | 2023-10-17 | 2023-10-17 | Automatic control system of high-pressure cell culture cabin |
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Cited By (1)
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| CN117467541A (en) * | 2023-11-02 | 2024-01-30 | 中国人民解放军海军特色医学中心 | Integrated high-pressure cell culture cabin control system |
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