CN114958607A - Compound microbial agent with high preparation efficiency and preparation method thereof - Google Patents
Compound microbial agent with high preparation efficiency and preparation method thereof Download PDFInfo
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- CN114958607A CN114958607A CN202210708682.6A CN202210708682A CN114958607A CN 114958607 A CN114958607 A CN 114958607A CN 202210708682 A CN202210708682 A CN 202210708682A CN 114958607 A CN114958607 A CN 114958607A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 77
- 238000002360 preparation method Methods 0.000 title claims abstract description 53
- 150000001875 compounds Chemical class 0.000 title claims abstract description 30
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 76
- 238000004140 cleaning Methods 0.000 claims abstract description 46
- 238000003756 stirring Methods 0.000 claims abstract description 38
- 239000000645 desinfectant Substances 0.000 claims abstract description 33
- 238000001035 drying Methods 0.000 claims abstract description 33
- 230000001954 sterilising effect Effects 0.000 claims abstract description 23
- 230000000249 desinfective effect Effects 0.000 claims abstract description 20
- 239000007921 spray Substances 0.000 claims abstract description 20
- 238000005507 spraying Methods 0.000 claims abstract description 20
- 230000002070 germicidal effect Effects 0.000 claims abstract description 13
- 241000894006 Bacteria Species 0.000 claims abstract description 11
- 239000000428 dust Substances 0.000 claims abstract description 10
- 239000012535 impurity Substances 0.000 claims abstract description 10
- 230000001678 irradiating effect Effects 0.000 claims abstract description 10
- 239000012530 fluid Substances 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims description 45
- 239000001963 growth medium Substances 0.000 claims description 36
- 238000000605 extraction Methods 0.000 claims description 21
- 238000002156 mixing Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 239000002994 raw material Substances 0.000 claims description 12
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 claims description 10
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 9
- 150000001720 carbohydrates Chemical class 0.000 claims description 9
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 9
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 9
- 239000011782 vitamin Substances 0.000 claims description 9
- 229940088594 vitamin Drugs 0.000 claims description 9
- 229930003231 vitamin Natural products 0.000 claims description 9
- 235000013343 vitamin Nutrition 0.000 claims description 9
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 239000003463 adsorbent Substances 0.000 claims description 3
- 239000003610 charcoal Substances 0.000 claims description 3
- 241000411851 herbal medicine Species 0.000 claims description 3
- 238000000034 method Methods 0.000 claims 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 description 7
- 241000700605 Viruses Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 150000003568 thioethers Chemical class 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 241000902900 cellular organisms Species 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/26—Accessories or devices or components used for biocidal treatment
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/087—Cleaning containers, e.g. tanks by methods involving the use of tools, e.g. brushes, scrapers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/093—Cleaning containers, e.g. tanks by the force of jets or sprays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/10—Apparatus features
- A61L2202/15—Biocide distribution means, e.g. nozzles, pumps, manifolds, fans, baffles, sprayers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Health & Medical Sciences (AREA)
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- Genetics & Genomics (AREA)
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Abstract
The invention discloses a compound microbial agent with high preparation efficiency and a preparation method thereof, and the compound microbial agent comprises the following steps: step a: selecting a culture dish, and selecting a culture dish with a proper size; step b: cleaning the culture dish, spraying cleaning fluid into the culture dish through a spray head, cleaning the culture dish by using a cleaning brush, and removing dust and impurities in the culture dish; step c: sterilizing and disinfecting the culture dish, spraying disinfectant into the culture dish through a spray head, stirring the disinfectant by using a stirring sheet to ensure that the disinfectant is fully contacted with the culture dish, fully removing bacteria in the culture dish, irradiating the culture dish by using an ultraviolet germicidal lamp, and sterilizing and disinfecting the culture dish; step d: and drying the culture dish. The invention realizes the purpose of efficiently preparing the compound microbial agent for removing the sulfide, and simultaneously solves the problem of low efficiency of the traditional preparation method.
Description
Technical Field
The invention relates to the technical field of compound microbial agents, in particular to a compound microbial agent with high preparation efficiency and a preparation method thereof.
Background
Microorganisms are a general term for all microorganisms which are difficult to see by the naked eye and can be observed by means of an optical microscope or an electron microscope, the microorganisms comprise bacteria, viruses, fungi, a few algae and the like, the viruses are non-cellular organisms consisting of a few components such as nucleic acid, protein and the like, but the survival of the viruses is dependent on living cells, according to different environments, the microbial agent is divided into space microorganisms, marine microorganisms and the like, and is divided into prokaryotic microorganisms and eukaryotic microorganisms according to cell structure classification, the compound microbial agent can be applied to neutralization reaction of sulfides, has obvious effect of removing sulfides and has the advantages of environmental protection and energy saving, however, in the existing preparation of the compound microbial agent, the preparation efficiency is reduced due to the influence of external environmental factors, so that the using effect of the prepared microbial inoculum is poor.
Disclosure of Invention
The invention aims to provide a compound microbial agent with high preparation efficiency and a preparation method thereof, which have the advantage of high preparation efficiency and solve the problem of low preparation efficiency of the traditional microbial agent.
In order to achieve the purpose, the invention provides the following technical scheme: a compound microbial agent with high preparation efficiency and a preparation method thereof comprise the following steps:
step a: selecting a culture dish, and selecting a culture dish with a proper size;
step b: cleaning the culture dish, spraying cleaning fluid into the culture dish through a spray head, cleaning the culture dish by using a cleaning brush, and removing dust and impurities in the culture dish;
step c: sterilizing and disinfecting the culture dish, spraying disinfectant into the culture dish through a spray head, stirring the disinfectant by using a stirring sheet to ensure that the disinfectant is fully contacted with the culture dish, fully removing bacteria in the culture dish, irradiating the culture dish by using an ultraviolet germicidal lamp, and sterilizing and disinfecting the culture dish;
step d: drying the culture dish, and drying the culture dish by using drying equipment;
step e: preparing a culture medium by using the following raw materials in parts by weight: mixing a carbohydrate: 2 parts of nitrogen-containing substance: 0.3 part of inorganic salt: 0.3 part, vitamin: 0.4 part of water: mixing 7 parts of the mixture, introducing the mixture into a culture dish, and stirring the mixture for 2-3 minutes by using a stirring rod to fully mix the mixture together to prepare a culture medium;
step f: adjusting the temperature and humidity of the incubator, placing the culture dish in the incubator, adjusting the temperature and humidity of the incubator by adjusting the turntable, and placing the culture medium for 3-7 days to finish the preparation of the microbial agent;
step g: and (3) extracting the microbial agent, wherein an extraction plate made of turf is in full contact with the microbial agent, so that a culture medium containing the microbial agent is fully adhered to the extraction plate, and the microbial agent is extracted.
Preferably, the cleaning liquid in the step b is a pure water cleaning liquid.
Preferably, the disinfectant in the step c is prepared from the following raw materials in parts by weight: sterilizing water: 0.2-0.5 parts of water: 9.5 to 9.8 portions.
Preferably, the irradiation time of the culture dish by the ultraviolet germicidal lamp in the step c is as follows: 10-20 minutes.
Preferably, the culture dish is dried by the drying equipment in the step d for 5-10 minutes.
Preferably, the extraction plate made of Chinese herbal medicine charcoal in the step g is used as an adsorbent.
Compared with the prior art, the invention has the following beneficial effects:
the invention realizes the purpose of efficiently preparing the compound microbial agent for removing the sulfide, and simultaneously solves the problem of low efficiency of the traditional preparation method.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
A compound microbial agent with high preparation efficiency and a preparation method thereof comprise the following steps:
step a: selecting a culture dish, and selecting a culture dish with a proper size;
step b: cleaning the culture dish, spraying cleaning liquid into the culture dish through a spray head, cleaning the culture dish by using a cleaning brush, and removing dust and impurities in the culture dish;
step c: sterilizing and disinfecting the culture dish, spraying disinfectant into the culture dish through a spray head, stirring the disinfectant by using a stirring sheet to ensure that the disinfectant is fully contacted with the culture dish, fully removing bacteria in the culture dish, irradiating the culture dish by using an ultraviolet germicidal lamp, and sterilizing and disinfecting the culture dish;
step d: drying the culture dish, and drying the culture dish by using drying equipment;
step e: preparing a culture medium by using the following raw materials in parts by weight: mixing a carbohydrate: 2 parts of nitrogen-containing substance: 0.3 part of inorganic salt: 0.3 part, vitamin: 0.4 part of water: mixing 7 parts of the mixture, introducing the mixture into a culture dish, and stirring the mixture for 2-3 minutes by using a stirring rod to fully mix the mixture together to prepare a culture medium;
step f: adjusting the temperature and humidity of the incubator, placing the culture dish in the incubator, adjusting the temperature and humidity of the incubator by adjusting the turntable, and placing the culture medium for 3-7 days to complete the preparation of the microbial agent;
step g: and (3) extracting the microbial agent, wherein an extraction plate made of turf is in full contact with the microbial agent, so that a culture medium containing the microbial agent is fully adhered to the extraction plate, and the microbial agent is extracted.
Example 1
A compound microbial agent with high preparation efficiency and a preparation method thereof comprise the following steps:
step a: selecting a culture dish, and selecting a culture dish with a proper size;
step b: cleaning the culture dish, spraying cleaning fluid into the culture dish through a spray head, cleaning the culture dish by using a cleaning brush, and removing dust and impurities in the culture dish;
step c: sterilizing and disinfecting the culture dish, spraying disinfectant into the culture dish through a spray head, stirring the disinfectant by using a stirring sheet to ensure that the disinfectant is fully contacted with the culture dish, fully removing bacteria in the culture dish, irradiating the culture dish by using an ultraviolet germicidal lamp, and sterilizing and disinfecting the culture dish;
step d: drying the culture dish, and drying the culture dish by using drying equipment;
step e: preparing a culture medium by using the following raw materials in parts by weight: mixing a carbohydrate: 2 parts of nitrogen-containing substance: 0.3 part of inorganic salt: 0.3 part, vitamin: 0.4 part of water: mixing 7 parts of the mixture, introducing the mixture into a culture dish, and stirring the mixture for 2-3 minutes by using a stirring rod to fully mix the mixture together to prepare a culture medium;
step f: adjusting the temperature and humidity of the incubator, placing the culture dish in the incubator, adjusting the temperature and humidity of the incubator by adjusting the turntable, and placing the culture medium for 3-7 days to finish the preparation of the microbial agent;
step g: and (3) extracting the microbial agent, wherein an extraction plate made of turf is in full contact with the microbial agent, so that a culture medium containing the microbial agent is fully adhered to the extraction plate, and the microbial agent is extracted.
Example 2
In example 1, the following additional steps were added:
and the cleaning liquid in the step b is pure water cleaning liquid.
A compound microbial agent with high preparation efficiency and a preparation method thereof comprise the following steps:
step a: selecting a culture dish, and selecting a culture dish with a proper size;
step b: cleaning the culture dish, spraying cleaning fluid into the culture dish through a spray head, cleaning the culture dish by using a cleaning brush, and removing dust and impurities in the culture dish;
step c: sterilizing and disinfecting the culture dish, spraying disinfectant into the culture dish through a spray head, stirring the disinfectant by using a stirring sheet to ensure that the disinfectant is fully contacted with the culture dish, fully removing bacteria in the culture dish, irradiating the culture dish by using an ultraviolet germicidal lamp, and sterilizing and disinfecting the culture dish;
step d: drying the culture dish, and drying the culture dish by using drying equipment;
step e: preparing a culture medium by using the following raw materials in parts by weight: mixing a carbohydrate: 2 parts of nitrogen-containing substance: 0.3 part of inorganic salt: 0.3 part, vitamin: 0.4 part of water: mixing 7 parts of the mixture, introducing the mixture into a culture dish, and stirring the mixture for 2-3 minutes by using a stirring rod to fully mix the mixture together to prepare a culture medium;
step f: adjusting the temperature and humidity of the incubator, placing the culture dish in the incubator, adjusting the temperature and humidity of the incubator by adjusting the turntable, and placing the culture medium for 3-7 days to finish the preparation of the microbial agent;
step g: and (3) extracting the microbial agent, wherein an extraction plate made of turf is in full contact with the microbial agent, so that a culture medium containing the microbial agent is fully adhered to the extraction plate, and the microbial agent is extracted.
Example 3
In example 2, the following additional steps were added:
the disinfectant in the step c is prepared from the following raw materials in parts by weight: sterilizing water: 0.2-0.5 parts of water: 9.5 to 9.8 portions.
A compound microbial agent with high preparation efficiency and a preparation method thereof comprise the following steps:
step a: selecting a culture dish, and selecting a culture dish with a proper size;
step b: cleaning the culture dish, spraying cleaning liquid into the culture dish through a spray head, cleaning the culture dish by using a cleaning brush, and removing dust and impurities in the culture dish;
step c: sterilizing and disinfecting the culture dish, spraying disinfectant into the culture dish through a spray head, stirring the disinfectant by using a stirring sheet to ensure that the disinfectant is fully contacted with the culture dish, fully removing bacteria in the culture dish, irradiating the culture dish by using an ultraviolet germicidal lamp, and sterilizing and disinfecting the culture dish;
step d: drying the culture dish, and drying the culture dish by using drying equipment;
step e: preparing a culture medium by using the following raw materials in parts by weight: mixing a carbohydrate: 2 parts of nitrogen-containing substance: 0.3 part of inorganic salt: 0.3 part, vitamin: 0.4 part of water: mixing 7 parts of the mixture, introducing the mixture into a culture dish, and stirring the mixture for 2-3 minutes by using a stirring rod to fully mix the mixture together to prepare a culture medium;
step f: adjusting the temperature and humidity of the incubator, placing the culture dish in the incubator, adjusting the temperature and humidity of the incubator by adjusting the turntable, and placing the culture medium for 3-7 days to complete the preparation of the microbial agent;
step g: and (3) extracting the microbial agent, wherein an extraction plate made of turf is in full contact with the microbial agent, so that a culture medium containing the microbial agent is fully adhered to the extraction plate, and the microbial agent is extracted.
Example 4
In example 3, the following steps were added:
in the step c, the irradiation time of the culture dish by the ultraviolet germicidal lamp is as follows: 10-20 minutes.
A compound microbial agent with high preparation efficiency and a preparation method thereof comprise the following steps:
step a: selecting a culture dish, and selecting a culture dish with a proper size;
step b: cleaning the culture dish, spraying cleaning fluid into the culture dish through a spray head, cleaning the culture dish by using a cleaning brush, and removing dust and impurities in the culture dish;
step c: sterilizing and disinfecting the culture dish, spraying disinfectant into the culture dish through a spray head, stirring the disinfectant by using a stirring sheet to ensure that the disinfectant is fully contacted with the culture dish, fully removing bacteria in the culture dish, irradiating the culture dish by using an ultraviolet germicidal lamp, and sterilizing and disinfecting the culture dish;
step d: drying the culture dish, and drying the culture dish by using drying equipment;
step e: preparing a culture medium by using the following raw materials in parts by weight: mixing a carbohydrate: 2 parts of nitrogen-containing substance: 0.3 part of inorganic salt: 0.3 part, vitamin: 0.4 part of water: mixing 7 parts of the mixture, introducing the mixture into a culture dish, and stirring the mixture for 2-3 minutes by using a stirring rod to fully mix the mixture together to prepare a culture medium;
step f: adjusting the temperature and humidity of the incubator, placing the culture dish in the incubator, adjusting the temperature and humidity of the incubator by adjusting the turntable, and placing the culture medium for 3-7 days to complete the preparation of the microbial agent;
step g: and (3) extracting the microbial agent, wherein an extraction plate made of turf is in full contact with the microbial agent, so that a culture medium containing the microbial agent is fully adhered to the extraction plate, and the microbial agent is extracted.
Example 5
In example 4, the following steps were added:
and d, drying the culture dish for 5-10 minutes by using drying equipment in the step d.
A compound microbial agent with high preparation efficiency and a preparation method thereof comprise the following steps:
step a: selecting a culture dish, and selecting a culture dish with a proper size;
step b: cleaning the culture dish, spraying cleaning fluid into the culture dish through a spray head, cleaning the culture dish by using a cleaning brush, and removing dust and impurities in the culture dish;
step c: sterilizing and disinfecting the culture dish, spraying disinfectant into the culture dish through a spray head, stirring the disinfectant by using a stirring sheet to ensure that the disinfectant is fully contacted with the culture dish, fully removing bacteria in the culture dish, irradiating the culture dish by using an ultraviolet germicidal lamp, and sterilizing and disinfecting the culture dish;
step d: drying the culture dish, and drying the culture dish by using drying equipment;
step e: preparing a culture medium by using the following raw materials in parts by weight: mixing a carbohydrate: 2 parts of nitrogen-containing substance: 0.3 part of inorganic salt: 0.3 part, vitamin: 0.4 part of water: mixing 7 parts of the mixture, introducing the mixture into a culture dish, and stirring the mixture for 2-3 minutes by using a stirring rod to fully mix the mixture together to prepare a culture medium;
step f: adjusting the temperature and humidity of the incubator, placing the culture dish in the incubator, adjusting the temperature and humidity of the incubator by adjusting the turntable, and placing the culture medium for 3-7 days to finish the preparation of the microbial agent;
step g: and (3) extracting the microbial agent, wherein an extraction plate made of turf is in full contact with the microbial agent, so that a culture medium containing the microbial agent is fully adhered to the extraction plate, and the microbial agent is extracted.
Example 6
In example 5, the following steps were added:
and g, using an extraction plate made of Chinese herbal medicine charcoal as an adsorbent.
A compound microbial agent with high preparation efficiency and a preparation method thereof comprise the following steps:
a, step a: selecting a culture dish, and selecting a culture dish with a proper size;
step b: cleaning the culture dish, spraying cleaning fluid into the culture dish through a spray head, cleaning the culture dish by using a cleaning brush, and removing dust and impurities in the culture dish;
step c: sterilizing and disinfecting the culture dish, spraying disinfectant into the culture dish through a spray head, stirring the disinfectant by using a stirring sheet to ensure that the disinfectant is fully contacted with the culture dish, fully removing bacteria in the culture dish, irradiating the culture dish by using an ultraviolet germicidal lamp, and sterilizing and disinfecting the culture dish;
step d: drying the culture dish, and drying the culture dish by using drying equipment;
step e: preparing a culture medium by using the following raw materials in parts by weight: mixing a carbohydrate: 2 parts of nitrogen-containing substance: 0.3 part of inorganic salt: 0.3 part, vitamin: 0.4 part of water: mixing 7 parts of the mixture, introducing the mixture into a culture dish, and stirring the mixture for 2-3 minutes by using a stirring rod to fully mix the mixture together to prepare a culture medium;
step f: adjusting the temperature and humidity of the incubator, placing the culture dish in the incubator, adjusting the temperature and humidity of the incubator by adjusting the turntable, and placing the culture medium for 3-7 days to finish the preparation of the microbial agent;
step g: and (3) extracting the microbial agent, wherein an extraction plate made of turf is in full contact with the microbial agent, so that a culture medium containing the microbial agent is fully adhered to the extraction plate, and the microbial agent is extracted.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A compound microbial agent with high preparation efficiency and a preparation method thereof are characterized in that: the method comprises the following steps:
step a: selecting a culture dish, and selecting a culture dish with a proper size;
step b: cleaning the culture dish, spraying cleaning fluid into the culture dish through a spray head, cleaning the culture dish by using a cleaning brush, and removing dust and impurities in the culture dish;
step c: sterilizing and disinfecting the culture dish, spraying disinfectant into the culture dish through a spray head, stirring the disinfectant by using a stirring sheet to ensure that the disinfectant is fully contacted with the culture dish, fully removing bacteria in the culture dish, irradiating the culture dish by using an ultraviolet germicidal lamp, and sterilizing and disinfecting the culture dish;
step d: drying the culture dish, and drying the culture dish by drying equipment;
step e: preparing a culture medium by using the following raw materials in parts by weight: mixing a carbohydrate: 2 parts of nitrogen-containing substance: 0.3 part of inorganic salt: 0.3 part, vitamin: 0.4 part of water: mixing 7 parts of the mixture, introducing the mixture into a culture dish, and stirring the mixture for 2-3 minutes by using a stirring rod to fully mix the mixture together to prepare a culture medium;
step f: adjusting the temperature and humidity of the incubator, placing the culture dish in the incubator, adjusting the temperature and humidity of the incubator by adjusting the turntable, and placing the culture medium for 3-7 days to finish the preparation of the microbial agent;
step g: and (3) extracting the microbial agent, wherein an extraction plate made of turf is in full contact with the microbial agent, so that a culture medium containing the microbial agent is fully adhered to the extraction plate, and the microbial agent is extracted.
2. The compound microbial agent with high preparation efficiency and the preparation method thereof according to claim 1, wherein the compound microbial agent is characterized in that: and the cleaning liquid in the step b is pure water cleaning liquid.
3. The compound microbial agent with high preparation efficiency and the preparation method thereof according to claim 1, wherein the compound microbial agent is characterized in that: the disinfectant in the step c is prepared from the following raw materials in parts by weight: sterilizing water: 0.2-0.5 parts of water: 9.5-9.8 parts.
4. The compound microbial agent with high preparation efficiency and the preparation method thereof according to claim 1, wherein the compound microbial agent is characterized in that: the irradiation time of the ultraviolet germicidal lamp to the culture dish in the step c is as follows: 10-20 minutes.
5. The compound microbial agent with high preparation efficiency and the preparation method thereof according to claim 1, wherein the compound microbial agent is characterized in that: and d, drying the culture dish for 5-10 minutes by the drying equipment in the step d.
6. The compound microbial agent with high preparation efficiency and the preparation method thereof according to claim 1, wherein the compound microbial agent is characterized in that: and g, using an extraction plate prepared from the Chinese herbal medicine charcoal as an adsorbent.
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| CN109136142A (en) * | 2018-09-10 | 2019-01-04 | 天津科技大学 | A kind of white yellow streptomycete and the method and application that Biocontrol microorganism microbial inoculum is prepared using the microorganism |
| CN110972956A (en) * | 2020-01-02 | 2020-04-10 | 昭通芸生农业科技开发有限公司 | Pleione seed tissue culture method |
| AU2020100857A4 (en) * | 2020-01-02 | 2020-07-02 | Recom (Qingdao) Biotechnology Co. Ltd | Method for in vitro screening in-vivo antibacterial activity phages |
| WO2021232431A1 (en) * | 2020-05-18 | 2021-11-25 | 安徽启威生物科技有限公司 | Degradable bactericide and preparation method therefor |
| WO2022121970A1 (en) * | 2020-12-10 | 2022-06-16 | 浙江景兴纸业股份有限公司 | Method for preparing bacteriostatic household paper |
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| CN109136142A (en) * | 2018-09-10 | 2019-01-04 | 天津科技大学 | A kind of white yellow streptomycete and the method and application that Biocontrol microorganism microbial inoculum is prepared using the microorganism |
| CN110972956A (en) * | 2020-01-02 | 2020-04-10 | 昭通芸生农业科技开发有限公司 | Pleione seed tissue culture method |
| AU2020100857A4 (en) * | 2020-01-02 | 2020-07-02 | Recom (Qingdao) Biotechnology Co. Ltd | Method for in vitro screening in-vivo antibacterial activity phages |
| WO2021232431A1 (en) * | 2020-05-18 | 2021-11-25 | 安徽启威生物科技有限公司 | Degradable bactericide and preparation method therefor |
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