CN103798521A - Organic microelement-containing biological feed composition, application thereof, and special compound premix and special feed of piglets - Google Patents
Organic microelement-containing biological feed composition, application thereof, and special compound premix and special feed of piglets Download PDFInfo
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Abstract
The invention relates to the field of feed, and in particular relates to an organic microelement-containing biological feed composition, application thereof and a special compound premix and a special feed of piglets. The biological feed composition comprises organic microelements and yeast metabolites, wherein the organic microelements comprise copper glycine, ferrous fumarate, zinc lactate, manganese methionine, selenium yeast and chromium nicotinate. The yeast metabolites are capable of promoting the synergistic effect of all the microelements and improving the utilization rate of the microelements; the feed prepared from the biological feed composition is good in palatability, high in absorption rate, free from potential risk of heavy metals, safe and environmentally friendly; after the feed prepared from the biological feed composition is used, the average daily gain is remarkably improved, the feed to gain ratio, the diarrhea rate and the content of microelements in excrement are remarkably reduced, the production performance of the piglets is improved, the intestinal health is improved, the feed conversion ratio is increased, and the skin color and the fur condition of the piglets are obviously improved.
Description
Technical field
The present invention relates to field of fodder, particularly containing the biological feedstuff composition of organic trace element and application thereof, piglet compound premix special, piglet special feed.
Background technology
Over nearly 20 years, China's feed industry fast development, is that production scale or product quality have all obtained significant progress.Trace mineral supplement as feed core part is also more and more paid attention to.In feed, need the trace element adding to comprise iron (Fe), copper (Cu), manganese (Mn), zinc (Zn), iodine (I) etc.Practice shows, the effect that in feed, the reasonable use of trace element can be played raising breeding performonce fo animals, improve animal skin and improve immunity.
At present, in feed, conventional trace element is inorganic microelement.But, the experimental results and application practice prove in recent years, and the long-term use of inorganic microelement in animal produces presented following drawback: absorptivity is low, palatability is poor, potential heavy metal risk, affect animal health, unstable chemcial property, a large amount of excretion and cause in environmental protection pressure, easy and feed the shortcomings such as vitamin isoreactivity composition generation chemical reaction.The inorganic microelement that particularly feed industry uses is now in the majority with sulfate, and the situation that ubiquity overdose uses, and both non-nutritive substances of sulfate ion, also non-metabolite, a large amount of existence of sulfate ion may increase the sour adhesion of feed, also may cause acid-base balance and the osmotic pressure imbalance of enteron aisle, and then cause piglet to produce diarrhoea.Because piglet is in early growth period, the immunologic function of body, enteron aisle digestive function etc. are all unsound, very low to the digestive utilization ratio of inorganic microelement, piglet ochrodermia, comparatively general by situations such as the thick unrest of hair.
A large amount of practice confirmations, organic trace element has plurality of advantages, can improve to a certain extent the health status of piglet.To be metallic element be combined by covalent bond or ionic bond the complex compound or the chelate that form with ligands such as protein, little peptide, amino acid, organic acid, polysaccharide derivates to organic trace element.Compared with inorganic microelement, there is following advantage in organic trace element:
(1) the biological utilisation height of tiring.Studies have shown that, organic trace element has higher bioavailability than inorganic salts, and growth, reproduction, health and feed conversion rate etc. to animal have obvious facilitation.
(2) steady chemical structure.Organic trace element is subject to the protection of ligand; be not vulnerable to the impact of the physical chemical factor that is unfavorable for metal absorption in intestines and stomach; can prevent that metal ion from forming insoluble compound with hydrochloric acid in gastric juice effect in alimentary canal; avoid with feed in the formation such as phytic acid, oxalic acid be difficult to the chelate absorbing; be subject to the impact of other inorganic ions and antagonist less; can stop the suction-operated of insoluble colloid, there is good chemical stability and biochemical stability.
(3) immunologic function strengthens.Organic trace element is conducive to close to the natural form of enzyme absorb, and after being absorbed, the trace element of complexing can be transported directly in specific target tissue and enzyme system, therefrom plays a role and meets body requirement.Organic trace element has enhancing premunition, improves immune response, promotes effect of zooblast and humoral immunity, bring into play disease-resistantly, anti-stress effect, improves animal skin situation, reduce body early embryo death, some enteritis, dermatitis, dysentery and basin blood are had to therapeutic action; Too high and change under the stressed conditions such as daily ration in inoculation, castration, transportation, temperature, there is good effect.
(4) good palatability and side effect are little.Organic trace element has double nutrition and is worth, as organic acid complex compound, can provide animal body needed organic acid, trace element can be provided again, good palatability, toxic and side effect is little, and security is good, and absorptivity is high, easily transhipment, can strengthen the activity of animal body endoenzyme, improve the utilization rate of protein, fat and vitamin, thereby promote the performance of growth of animal performance.Little to alimentary canal ambient influnence, be conducive to digestive environments normal.Acid adhesion is low, is conducive to stomach acidity environment normally and the performance of acidulant effect.Be difficult for dissociating, digestive enzyme is lived and do not affected.Alimentary canal epithelium and fine hair are not had to detrimental effect, guarantee the normal of digestion physical environment, improve eupepsy and absorbability.
(5) with feed in other composition influence each other little.Organic trace element ion is closed in the chela ring of chelate, has greatly reduced the oxidation of the vitamin to adding in feed; Chelate has protected trace element not seized and discharge by phytic acid; avoid the antagonism of the interior a large amount of divalent calcium ions of alimentary canal and metal trace element; make metal trace element arrive smoothly absorption site; relatively improved trace element retaining and utilizing in body, and digest and assimilate and mobilization utilize speed all greatly improve.
(6) absorptivity is beneficial to well environmental protection.In organic trace element, metal ion, under the protection of ligand, forms stable chemical constitution, has both avoided the mutual antagonism between mineral matter, has eliminated again the easily drawback to vitamin oxidation of inorganic salts.Ligand has protected trace element not seized and discharge by phytic acid; avoid the antagonism of the interior a large amount of divalent calcium ions of alimentary canal and metal trace element; make metal trace element arrive smoothly absorption site; relatively improved trace element retaining and utilizing in body, and digest and assimilate and mobilization utilize speed all greatly improve.Experiment shows, the absorptivity of organic trace element be mineral sulfates 2-4 doubly.
A large amount of result of study and application practices show, organic trace element has outstanding effect: improve breeding performonce fo animals, improve feed efficiency; Promote animal intestinal health, improve immunity and premunition, alleviation stress; Participate in hormone regulating and controlling, improve reproductive performance; Safeguard epithelial tissue integrality, be beneficial to limb hoof health, improve animal colour of skin hair condition; Promote animal product quality, promote human health.
But the absorptivity of organic trace element does not reach perfect condition yet, in production practices, there is the phenomenon that feed conversion rate is low, grice diarrhoea rate is higher, coat condition is poor, greatly limit its application in feed industry.Therefore how improving the utilization rate of organic trace element, thereby improve food conversion ratio, pigling immunity, improve the coat condition of piglet, is the focus of studying in feedstuff industry.
Summary of the invention
In view of this, the invention provides containing the biological feedstuff composition of organic trace element and application thereof, piglet compound premix special, piglet special feed.In biological feedstuff composition, the interpolation of yeast metabolism product can promote the synergistic performance of each trace element, improves micro-utilization rate; The feed good palatability, the absorptivity that adopt biological feedstuff composition provided by the invention to make are high, without potential heavy metal risk, safety and environmental protection, can significantly improve average daily gain, the content that significantly reduces trace element in material anharmonic ratio, diarrhea rate and excrement, has improved the production performance of piglet, can improve intestinal health, improve food conversion ratio, can obviously improve the colour of skin and hair condition.
In order to realize foregoing invention object, the invention provides following technical scheme:
The invention provides a kind of biological feedstuff composition, include organic microelement and yeast metabolism product;
Organic trace element comprises cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium and nicotinate chromium;
The preparation method of yeast metabolism product is: get yeast culture and pulverize, take water as solvent extraction, obtain extract; Extract, through concentrated, centrifugal, is collected supernatant; Supernatant is purified, concentrated, dry, to obtain final product.
In the present invention, organic trace element, by preferred feedstock and science compatibility, can improve micro-utilization rate.Yeast metabolism product and organic trace element performance synergy, promote the efficient utilization of trace element.Feed good palatability, absorptivity that biological feedstuff composition provided by the invention makes are high, without potential heavy metal risk, safety and environmental protection, can significantly improve average daily gain, the content that significantly reduces trace element in material anharmonic ratio, diarrhea rate and excrement, has improved the production performance of piglet, can improve intestinal health, improve food conversion ratio, can obviously improve the colour of skin and hair condition.
Organic trace element refers to the compound that micro-inorganic salts and organic matter and catabolite thereof form.In the present invention, organic trace element comprises cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium and nicotinate chromium.
Cupric glycinate is synthetic as raw material take glycine and copper sulphate, and the copper of 1 mole is combined with the glycine of 1-2 mole, forms cupric glycinate, and glycine and copper optimum condition are part mol ratio 2:1, and chelation percent is more than 90%.Cupric glycinate absorbs by amino acid pinocytosis formula, alleviates the competition antagonism while absorption between trace element, not only greatly improves the absorption rate of copper ion, the absorption that also improves other trace element.Cupric glycinate good palatability, little to alimentary canal stimulation, stable chemical nature, biologically active is high, and additive capacity is little, is conducive to environmental protection.Compared with the organic copper of other form, cupric glycinate is easy to detect, and cost performance is the highest.
Ferrous fumarate is mainly prepared by fumaric acid and ferrous sulfate, and ferrous fumarate has the growth of the pig of promotion, regulates immunologic function, effectively prevents anaemia and improve the nutrition physiology effects such as colour of skin hair condition.The ferrous fumarate of pharmaceutical grade is the one for the treatment of anaemia medicine.Compared with inorganic iron, there is good palatability, stable chemical nature, the feature that biologically active is high.Compared with the Organic Iron of other form, do not contain inorganic sulfur acid ion, ferrous iron effective content, up to 31%, has high cost performance.Ferrous fumarate has national standard, and detection method is simple.
Zinc lactate up to 21.5%, is widely used in food industry containing zinc.In recent years, as progressively large-area applications in feed industry of new feed additive.Have safety, efficient advantage compared with inorganic zinc, good palatability, has no stimulation to alimentary canal; Double nutrition effect, can provide animal body needed lactate, and zinc can be provided again.Zinc lactate not only has and the similar function of amino-acid zinc and feature, and zinc content is higher, and production cost is lower, not sulphate-containing ion.Feed addictive zinc lactate has national standard, and detection method is simple.The factors such as complex art, economy, ecology and environmental protection, zinc lactate is one of optimal organic zinc source on the market at present.
Manganese methionine is to be primary raw material by methionine and inorganic manganese salt, makes by complex reaction.The Major Nutrient physiological action of manganese is as the enzyme activation factor or part in carbohydrate, lipid, protein and cholesterol metabolic.In addition, manganese is to maintain the requisite material of brain eubolism function.Manganese methionine is for body provides methionine when manganese source is provided, and methionine, as swine rations second or the 3rd limiting amino acid, has important biochemical functions.
Yeast selenium provides trace elements of selenium, and its most important nutrition physiology effect is to participate in glutathione peroxidase composition, and hydrogen in body or lipoperoxide are had to stronger reduction, and Cell protection membrane structure is complete and function is normal.Inorganic selenium main source sodium selenite, toxicity is large, mixes inhomogeneously in feed, easily causes animal poisoning.Yeast selenium is as a kind of Organic Selenium source of native form, safe and reliable, easily absorbs.Compared with the Organic Selenium of other form, the selenium in yeast selenium exists with the form of selenomethionine, with selenium in animal body utilize form consistent, bioavilability is high, and yeast selenium is easy to detect, and adds cost low.
Nicotinate chromium provides trace element chromium, and chromium is glucose tolerance factor, has the biologically active of similar insulin, to regulating carbohydrate, fat and protein metabolism to play an important role; Contribute to animal body intracellular metabolite, opposing stress affect simultaneously.In the chemical constitution of nicotinate chromium and body, the activity form glucose tolerance factor of chromium is the most approaching.Great many of experiments and application practice show, nicotinate chromium effect is better than or is equal to the Organic Chromium of other form, the bioavailability that nicotinate chromium has had more than the chromium in any other source, the absorption of mouse to chromium in nicotinate chromium and retaining apparently higher than chromium picolinate.Meanwhile, the nicotinate chromium price of same amount is only 1/3 of chromium picolinate, 1/4 of yeast chromium, and cost performance is very outstanding, and is easy to laboratory detection.
Yeast culture is according to microbial metabolism theory and applying biological fermentation engineering, the micro-ecological goods that formed after degree of depth fermentation on specific culture medium by saccharomycete under the production technology environment meticulously arranging.After producing by fermentation, the end product that yeast culture forms is a mixture that has comprised a small amount of yeast cells, variation culture medium and extracellular metabolic product.Yeast metabolism product mainly contains little peptide, nucleotides, organic acid, oligosaccharides, amino acid, nucleotides, ester class, alcohols, enzyme, vitamin etc.Studies confirm that, it is high that yeast metabolism product has biologically active, regulating animal intestinal function, promoting to have significant effect aspect micro-digestibility and utilization and guarantee animal health.Meanwhile, the steady quality of yeast metabolism product, standard is perfect.
As preferably, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is (5～12.5): (15～40): (22.5～55): (4～10): (0.075～0.13): (0.04～0.09): (40～60).
In embodiment more provided by the invention, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is 7.5:30:40:7.5:0.075:0.05:40.
In other embodiment provided by the invention, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is 12.5:40:37.5:10:0.125:0.09:60.
In other embodiment provided by the invention, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is 10:30:32.5:9:0.1:0.06:50.
In embodiment more provided by the invention, in the preparation method of yeast metabolism product, the fineness that yeast culture is pulverized is less than 10 μ m, after pulverizing, obtains Ultramicro-powder.
In embodiment more provided by the invention, in L/kg, obtain extract and centrifugal between concentration step after ratio between the volume of concentrate and the quality of yeast culture that obtain be 5:1.
In embodiment more provided by the invention, in the preparation method of yeast metabolism product, centrifugal rotating speed is 10000 revs/min.
In embodiment more provided by the invention, purifying is specially: with ethanol elution, collect ethanol eluate.
In embodiment more provided by the invention, in the preparation method of yeast metabolism product, wash-out pillar used is large pore resin absorption column.
The present invention also provides the application of this biological feedstuff composition in preparation piglet compound premix special or piglet special feed; This biological feedstuff composition includes organic microelement and yeast metabolism product; Organic trace element comprises cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium and nicotinate chromium; The preparation method of yeast metabolism product is: get yeast culture and pulverize, take water as solvent extraction, obtain extract; Extract, through concentrated, centrifugal, is collected supernatant; Supernatant is purified, concentrated, dry, to obtain final product; As preferably, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is (5～12.5): (15～40): (22.5～55): (4～10): (0.075～0.13): (0.04～0.09): (40～60); In embodiment more provided by the invention, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is 7.5:30:40:7.5:0.075:0.05:40; In other embodiment provided by the invention, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is 12.5:40:37.5:10:0.125:0.09:60; In other embodiment provided by the invention, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is 10:30:32.5:9:0.1:0.06:50; In embodiment more provided by the invention, in the preparation method of yeast metabolism product, the fineness that yeast culture is pulverized is less than 10 μ m, after pulverizing, obtains Ultramicro-powder; In embodiment more provided by the invention, in L/kg, obtain extract and centrifugal between concentration step after ratio between the volume of concentrate and the quality of yeast culture that obtain be 5:1; In embodiment more provided by the invention, in the preparation method of yeast metabolism product, centrifugal rotating speed is 10000 revs/min; In embodiment more provided by the invention, purifying is specially: with ethanol elution, collect ethanol eluate; In embodiment more provided by the invention, in the preparation method of yeast metabolism product, wash-out pillar used is large pore resin absorption column.
The present invention also provides a kind of piglet compound premix special, comprises carrier, antioxidant and biological feedstuff composition provided by the invention; This biological feedstuff composition includes organic microelement and yeast metabolism product; Organic trace element comprises cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium and nicotinate chromium; The preparation method of yeast metabolism product is: get yeast culture and pulverize, take water as solvent extraction, obtain extract; Extract, through concentrated, centrifugal, is collected supernatant; Supernatant is purified, concentrated, dry, to obtain final product; As preferably, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is (5～12.5): (15～40): (22.5～55): (4～10): (0.075～0.13): (0.04～0.09): (40～60); In embodiment more provided by the invention, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is 7.5:30:40:7.5:0.075:0.05:40; In other embodiment provided by the invention, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is 12.5:40:37.5:10:0.125:0.09:60; In other embodiment provided by the invention, organic trace element is in trace element, and the mass ratio of cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium, nicotinate chromium and yeast metabolism product is 10:30:32.5:9:0.1:0.06:50; In embodiment more provided by the invention, in the preparation method of yeast metabolism product, the fineness that yeast culture is pulverized is less than 10 μ m, after pulverizing, obtains Ultramicro-powder; In embodiment more provided by the invention, in L/kg, obtain extract and centrifugal between concentration step after ratio between the volume of concentrate and the quality of yeast culture that obtain be 5:1; In embodiment more provided by the invention, in the preparation method of yeast metabolism product, centrifugal rotating speed is 10000 revs/min; In embodiment more provided by the invention, purifying is specially: with ethanol elution, collect ethanol eluate; In embodiment more provided by the invention, in the preparation method of yeast metabolism product, wash-out pillar used is large pore resin absorption column.
As preferably, the quality percentage composition that biological feedstuff composition accounts for piglet compound premix special is 28%～65%.
Preferably, to account for the quality percentage composition of piglet compound premix special be 40%～55% to biological feedstuff composition.
In order to protect organic trace element and yeast metabolism product in piglet compound premix special not oxidized, must add antioxidant.As preferably, the quality percentage composition that antioxidant accounts for piglet compound premix special is 0.02%～0.06%, and surplus is carrier.
Preferably, the quality percentage composition that antioxidant accounts for piglet compound premix special is 0.04%～0.06%, and surplus is carrier.
In embodiment more provided by the invention, antioxidant is a kind of in ethoxyquinoline or dibutyl hydroxy toluene or both mixtures.
Carrier is that one can be carried trace active composition, improves its dispersiveness, and has the raised material of good chemical stability and adsorptivity.After trace active composition is carried by carrier, the some physical characteristics of itself change or no longer show, and the physical property of gained mixture (as mobility, granularity etc.) depends on or shows as the characteristic of carrier substantially, requirement to carrier characteristics is: 1. carrier, originally as nonactive material, has good adsorption capacity and do not damage its activity carried micro constitutent; 2. coordinate the primary raw material of raising in powder to have good mixed characteristic to full price; 3. chemical stability is good, does not have pharmacologically active; 4. cheap.Conventional carrier has two classes, i.e. organic carrier and inorganic carrier.Inorganic carrier is calcium carbonate, calcium phosphate, silicate, silica, salt, potter's clay, talcum, vermiculite, zeolite powder, sepiolite powder etc., and this class carrier is used for the making of microelement pre-mix material.In embodiment more provided by the invention, carrier is a kind of of stone flour, bentonite or zeolite powder or the mixture more than both.
The present invention also provides a kind of piglet special feed, comprises basal diet and piglet compound premix special provided by the invention; This piglet compound premix special comprises carrier, antioxidant and biological feedstuff composition provided by the invention; As preferably, the quality percentage composition that biological feedstuff composition accounts for piglet compound premix special is 28%～65%; Preferably, to account for the quality percentage composition of piglet compound premix special be 40%～55% to biological feedstuff composition; As preferably, the quality percentage composition that antioxidant accounts for piglet compound premix special is 0.02%～0.06%, and surplus is carrier; Preferably, the quality percentage composition that antioxidant accounts for piglet compound premix special is 0.04%～0.06%, and surplus is carrier; In embodiment more provided by the invention, antioxidant is a kind of in ethoxyquinoline or dibutyl hydroxy toluene or both mixtures; In embodiment more provided by the invention, carrier is a kind of of stone flour, bentonite or zeolite powder or the mixture more than both.
As preferably, the quality percentage composition that piglet compound premix special accounts for piglet special feed is 0.2%～0.4%.
In embodiment more provided by the invention, take corn-soybean meal diet as basal diet.Corn-soybean meal diet comprises corn, dregs of beans, wheat bran, imported fish meal, calcium monohydrogen phosphate, stone flour, amino acid and vitamin premix.
The invention provides containing the biological feedstuff composition of organic trace element and application thereof, piglet compound premix special, piglet special feed.This biological feedstuff composition includes organic microelement and yeast metabolism product; Organic trace element comprises cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium and nicotinate chromium; The preparation method of yeast metabolism product is: get yeast culture and pulverize, take water as solvent extraction, obtain extract; Extract, through concentrated, centrifugal, is collected supernatant; Supernatant is purified, concentrated, dry, to obtain final product.Test by feeding effect, compare with inorganic microelement feed with the feed that does not add yeast metabolism product, the average daily gain of piglet special feed provided by the invention improve respectively 6.4%～6.5% and 7.2%～8.1%(p<0.05), material anharmonic ratio decline respectively 4.0%～4.9% and 5.5%～5.8%(p<0.05), diarrhea rate reduces respectively 14.3%～23.5% and 20%～27.8%, in pig manure, copper bar output reduces respectively 21.9%～26.3% and 25.3%～29.1%, in pig manure zinc discharge rate reduce respectively 27.4%～32.3% and 31%～34%(p<0.05), in pig manure iron discharge rate reduce respectively 15.7%～21.6% and 19%～23.1%(p<0.05), colour of skin scoring improve respectively 33.3%～34.3% and 37.5%～40%(p<0.05), hair condition scoring improve respectively 23.5%～28.6% and 26.5%～31.4%(p<0.05).As can be seen here, yeast metabolism product can promote the synergistic performance of each trace element, improves micro-utilization rate; The feed good palatability, the absorptivity that adopt biological feedstuff composition provided by the invention to make are high, without potential heavy metal risk, safety and environmental protection, can significantly improve average daily gain, the content that significantly reduces trace element in material anharmonic ratio, diarrhea rate and excrement, has improved the production performance of piglet, can improve intestinal health, improve food conversion ratio, can obviously improve the colour of skin and hair condition.
The specific embodiment
The invention discloses containing the biological feedstuff composition of organic trace element and application thereof, piglet compound premix special, piglet special feed, those skilled in the art can use for reference content herein, suitably improve technological parameter and realize.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the artly, they are all deemed to be included in the present invention.Method of the present invention and application are described by preferred embodiment, related personnel obviously can change methods and applications as herein described in content of the present invention, spirit and scope or suitably change and combination not departing from, and realizes and apply the technology of the present invention.
In biological feedstuff composition containing organic trace element provided by the invention and application thereof, piglet compound premix special, piglet special feed, raw materials used or auxiliary material all can be buied by market.Wherein, cupric glycinate, ferrous fumarate, zinc lactate, nicotinate chromium, the special amino acid of piglet and vitamin premix and inorganic microelement premix adopt Sichuan Animtech Feed Co., Ltd.'s product; Yeast selenium and yeast culture are purchased from Da Nongwei feed company of U.S. product; Ethoxyquinoline adopts Yixing Tainshi Feed Co., Ltd product; Dibutyl hydroxy toluene adopts Bayer A.G's product.
Below in conjunction with embodiment, further set forth the present invention:
The preparation of embodiment 1 biological feedstuff composition
The preparation method of yeast metabolism product is: get yeast culture and pulverize, take water as solvent extraction, obtain extract; Extract, through concentrated, centrifugal, is collected supernatant; Supernatant is purified, concentrated, dry, to obtain final product.
Organic trace element is in trace element, get cupric glycinate (in copper 7.5kg), ferrous fumarate (in iron 30kg), zinc lactate (in zinc 40kg), manganese methionine (in manganese 7.5kg), yeast selenium (in selenium 75g) and nicotinate chromium (in chromium 50g), the above-mentioned yeast metabolism product 40kg making, mix, to obtain final product.
The preparation of embodiment 2 biological feedstuff compositions
By yeast culture ultramicro grinding, (diameter is less than 10 μ m), obtains the Ultramicro-powder of yeast culture, extracts take water as solvent, obtains extract; Extract is concentrated, in L/kg, ratio between the volume of concentrate and the quality of yeast culture obtaining after concentrated is that 5:1(is equivalent to 1kg yeast culture and makes 5L concentrate), 10000 revs/min of high speed centrifugations, supernatant after centrifugal is adsorbed with large pore resin absorption column, collect ethanol elution part, obtain ethanol eluate, be dried through concentrated and spraying, obtain yeast metabolism product.
Organic trace element is in trace element, get cupric glycinate (in copper 12.5kg), ferrous fumarate (in iron 40kg), zinc lactate (in zinc 37.5kg), manganese methionine (in manganese 10kg), yeast selenium (in selenium 125g) and nicotinate chromium (in chromium 90g), the above-mentioned yeast metabolism product 60kg making, mix, to obtain final product.
The preparation of embodiment 3 biological feedstuff compositions
By yeast culture ultramicro grinding, (diameter is less than 10 μ m), obtains the Ultramicro-powder of yeast culture, extracts take water as solvent, obtains extract; Extract is concentrated, in L/kg, ratio between the volume of concentrate and the quality of yeast culture obtaining after concentrated is that 5:1(is equivalent to 1kg yeast culture and makes 5L concentrate), 10000 revs/min of high speed centrifugations, supernatant after centrifugal is adsorbed with large pore resin absorption column, collect ethanol elution part, obtain ethanol eluate, be dried through concentrated and spraying, obtain yeast metabolism product.
Organic trace element is in trace element, get cupric glycinate (in copper 10kg), ferrous fumarate (in iron 30kg), zinc lactate (in zinc 32.5kg), manganese methionine (in manganese 9kg), yeast selenium (in selenium 100g) and nicotinate chromium (in chromium 60g), the above-mentioned yeast metabolism product 50kg making, mix, to obtain final product.
The preparation of embodiment 4 biological feedstuff compositions
By yeast culture ultramicro grinding, (diameter is less than 10 μ m), obtains the Ultramicro-powder of yeast culture, extracts take water as solvent, obtains extract; Extract is concentrated, in L/kg, ratio between the volume of concentrate and the quality of yeast culture obtaining after concentrated is that 5:1(is equivalent to 1kg yeast culture and makes 5L concentrate), 10000 revs/min of high speed centrifugations, supernatant after centrifugal is adsorbed with large pore resin absorption column, collect ethanol elution part, obtain ethanol eluate, be dried through concentrated and spraying, obtain yeast metabolism product.
Organic trace element is in trace element, get cupric glycinate (in copper 5kg), ferrous fumarate (in iron 15kg), zinc lactate (in zinc 55kg), manganese methionine (in manganese 4kg), yeast selenium (in selenium 130g) and nicotinate chromium (in chromium 40g), the above-mentioned yeast metabolism product 55kg making, mix, to obtain final product.
The preparation of embodiment 5 biological feedstuff compositions
By yeast culture ultramicro grinding, (diameter is less than 10 μ m), obtains the Ultramicro-powder of yeast culture, extracts take water as solvent, obtains extract; Extract is concentrated, in L/kg, ratio between the volume of concentrate and the quality of yeast culture obtaining after concentrated is that 5:1(is equivalent to 1kg yeast culture and makes 5L concentrate), 10000 revs/min of high speed centrifugations, supernatant after centrifugal is adsorbed with large pore resin absorption column, collect ethanol elution part, obtain ethanol eluate, be dried through concentrated and spraying, obtain yeast metabolism product.
Organic trace element is in trace element, get cupric glycinate (in copper 12kg), ferrous fumarate (in iron 20kg), zinc lactate (in zinc 22.5kg), manganese methionine (in manganese 5kg), yeast selenium (in selenium 90g) and nicotinate chromium (in chromium 70g), the above-mentioned yeast metabolism product 60kg making, mix, to obtain final product.
The preparation of embodiment 6 piglet compound premix specials
Get the biological feedstuff composition 300kg that embodiment 1 makes, antioxidant ethoxyquinoline 500g, supplies 1000kg with zeolite powder, mixes, and to obtain final product.
The preparation of embodiment 7 piglet compound premix specials
Get the biological feedstuff composition 280kg that embodiment 2 makes, antioxidant ethoxyquinoline 600g, supplies 1000kg with stone flour, mixes, and to obtain final product.
The preparation of embodiment 8 piglet compound premix specials
Get the biological feedstuff composition 400kg that embodiment 3 makes, dibutyl hydroxy toluene 200g, ethoxyquinoline 200g, supplies 1000kg with zeolite powder, mixes, and to obtain final product.
The preparation of embodiment 9 piglet compound premix specials
Get the biological feedstuff composition 500kg that embodiment 4 makes, antioxidant ethoxyquinoline 300g, supplies 1000kg with stone flour, mixes, and to obtain final product.
The preparation of embodiment 10 piglet compound premix specials
Get the biological feedstuff composition 650kg that embodiment 5 makes, antioxidant dibutyl hydroxy toluene 200g, supplies 1000kg with bentonite, mixes, and to obtain final product.
The preparation of embodiment 11 piglet special feeds
The formula of piglet special feed is as shown in table 1.
The formula of table 1 piglet special feed
Get the piglet compound premix special 3kg that embodiment 6 makes and mix with basal diet 997kg, to obtain final product.
The preparation of embodiment 12 piglet special feeds
The formula of piglet special feed is as shown in table 2.
The formula of table 2 piglet special feed
Get the piglet compound premix special 4kg that embodiment 7 makes and mix with basal diet 996kg, to obtain final product.
The preparation of embodiment 13 piglet special feeds
The formula of piglet special feed is as shown in table 3.
The formula of table 3 piglet special feed
Get the piglet compound premix special 4kg that embodiment 8 makes and mix with basal diet 996kg, to obtain final product.
The preparation of embodiment 14 piglet special feeds
The basal diet 996.5kg that getting piglet compound premix special 3.5kg that embodiment 9 makes provides with embodiment 11 mixes, and to obtain final product.
The preparation of embodiment 15 piglet special feeds
The basal diet 998kg that getting piglet compound premix special 2kg that embodiment 10 makes provides with embodiment 12 mixes, and to obtain final product.
Embodiment 16 feeding effect tests
Test is divided into test group and control group, and control group comprises the first control group and the second control group.The piglet special feed that test group adopts embodiment 11 to provide, the first control group adopts the first control feed to feed, and the second control group adopts the second control feed to feed.
The preparation method of the first control feed is: organic trace element is in trace element, get cupric glycinate (in copper 7.5kg), ferrous fumarate (in iron 30kg), zinc lactate (in zinc 40kg), manganese methionine (in manganese 7.5kg), yeast selenium (in selenium 75g) and nicotinate chromium (in chromium 50g), mix, obtain premix semi-finished product.Get the premix semi-finished product 300kg making, antioxidant ethoxyquinoline 500g, supplies 1000kg with zeolite powder, mixes, and obtains premix.The basal diet 997kg that getting this premix 3kg and embodiment 11 provides mixes, and to obtain final product.Compared with the piglet special feed providing with embodiment 11, in the first control feed, do not add yeast metabolism product.
The second control feed is that inorganic microelement premix and basal diet mix, in the piglet special feed that its trace element, antioxidant, carrier, basal diet and content provide with embodiment 11, corresponding component and content are consistent, difference is that the trace element that the second control feed adopts is inorganic microelement, and does not add yeast metabolism product.
The three way cross piglet 360 (male and females half and half) of choosing average weight 15kg carries out, and according to body weight, principle that sex is consistent, is divided at random 3 groups, and every processing 6 repeats, 20 pigs of each repetition.Experimental group and control group are take NRC(2012) pig nutritional requirements is standard, take corn-soybean meal diet as basal diet.Test piglet body weight reaches 35kg and finishes to use.Before test, thoroughly clean column home, use 2%NaOH solution to rinse colony house, then the formalin of use and potassium permanganate are pressed stifling a few hours of 2:1, and ventilation cleaning, after one week, starts test.Before formal test starts, carry out the prerun of 7 days, between preliminary trial period, test pig is carried out to expelling parasite, epidemic prevention.Just trying every day phase 8:00 and 17:00 timing throw to powder-material, free choice feeding and drinking-water, observe, record pig health status, records full phase feed intake.Experimental period,, testing index comprised: copper, zinc and iron content in average daily gain, material anharmonic ratio, diarrhea rate, pig manure, colour of skin scoring, the scoring of hair condition.
Index calculating method:
Average daily gain=(off-test body weight-initial body weight)/test number of days
Material anharmonic ratio=average daily material consumption (kg/d/ head)/average daily gain (kg/d/ head)
Total number * 100% of diarrhea rate=diarrhea rate pig's head number/pig
Colour of skin methods of marking: general 1 point, more ruddy 2 points, ruddy 3 points, very ruddy 4 points, the more ruddy scoring of the colour of skin is higher.
Hair condition methods of marking: long and close 1 point, long, close 2 points, shorter, dredge 3 points, very short, dredge 4 points, shorter, the thinner scoring of hair is higher.
Result is as shown in table 4.
Table 4 weaned piglets and the comparison of colour of skin hair condition
Note: the different letter representation significant differences of same column shoulder motes (P<0.05), the shoulder motes identical expression there was no significant difference of letter (P>0.05).
Result of the test by table 4 can draw, compare with the second control group with the first control group, test group average daily gain improve respectively 6.4% and 7.2%(P<0.05), material anharmonic ratio decline respectively 4.0% and 5.5%(P<0.05), diarrhea rate reduces respectively 14.3% and 20%, in pig manure, copper bar output reduces respectively 22.1% and 25.3%, in pig manure zinc discharge rate reduce respectively 27.4% and 31%(P<0.05), in pig manure iron discharge rate reduce respectively 15.7% and 19%(P<0.05), colour of skin scoring improve respectively 33.3% and 37.5%(P<0.05), hair condition scoring improve respectively 25.9% and 26.5%(P<0.05).Visible, the daily gain of test group, material anharmonic ratio, excrement zinc, excrement iron, colour of skin scoring and the scoring of hair condition are significantly better than the first control group and the second control group, indices there are no significant difference between the first control group and the second control group.Result shows that yeast metabolism product can promote the synergistic performance of each trace element, improves micro-utilization rate; The feed (the piglet special feed that embodiment 11 provides) that the biological feedstuff composition that the embodiment of the present invention 1 provides makes can significantly improve average daily gain, significantly reduces the content of trace element in material anharmonic ratio, diarrhea rate and excrement, can obviously improve the colour of skin and hair condition.
Embodiment 17 feeding effect tests
Test is divided into test group and control group, and control group comprises the first control group and the second control group.The piglet special feed that test group adopts embodiment 12 to provide, the first control group adopts the first control feed to feed, and the second control group adopts the second control feed to feed.
The preparation method of the first control feed is: organic trace element is in trace element, get cupric glycinate (in copper 12.5kg), ferrous fumarate (in iron 40kg), zinc lactate (in zinc 37.5kg), manganese methionine (in manganese 10kg), yeast selenium (in selenium 125g) and nicotinate chromium (in chromium 90g), mix, obtain premix semi-finished product.Get the premix semi-finished product 280kg making, antioxidant ethoxyquinoline 600g, supplies 1000kg with stone flour, mixes, and obtains premix.The basal diet 996kg that getting this premix 4kg and embodiment 12 provides mixes, and to obtain final product.Compared with the piglet special feed providing with embodiment 12, in the first control feed, do not add yeast metabolism product.
The second control feed is that inorganic microelement premix and basal diet mix, in the piglet special feed that its trace element, antioxidant, carrier, basal diet and content provide with embodiment 12, corresponding component and content are consistent, difference is that the trace element that the second control feed adopts is inorganic microelement, and does not add yeast metabolism product.
The three way cross piglet 480 (male and females half and half) of choosing average weight 15kg carries out, and according to body weight, principle that sex is consistent, is divided at random 3 groups, and every processing 6 repeats, 30 pigs of each repetition.Experimental group and control group are take NRC(2012) pig nutritional requirements is standard, take corn-soybean meal diet as basal diet.Test piglet body weight reaches 35kg and finishes to use.Before test, thoroughly clean column home, use 2%NaOH solution to rinse colony house, then the formalin of use and potassium permanganate are pressed stifling a few hours of 2:1, and ventilation cleaning, after one week, starts test.Before formal test starts, carry out the prerun of 7 days, between preliminary trial period, test pig is carried out to expelling parasite, epidemic prevention.Just trying every day phase 8:00 and 17:00 timing throw to powder-material, free choice feeding and drinking-water, observe, record pig health status, records full phase feed intake.Experimental period,, testing index comprised: copper, zinc and iron content in average daily gain, material anharmonic ratio, diarrhea rate, pig manure, colour of skin scoring, the scoring of hair condition.
Index calculating method:
Average daily gain=(off-test body weight-initial body weight)/test number of days
Material anharmonic ratio=average daily material consumption (kg/d/ head)/average daily gain (kg/d/ head)
Total number * 100% of diarrhea rate=diarrhea rate pig's head number/pig
Colour of skin methods of marking: general 1 point, more ruddy 2 points, ruddy 3 points, very ruddy 4 points, the more ruddy scoring of the colour of skin is higher.
Hair condition methods of marking: long and close 1 point, long, close 2 points, shorter, dredge 3 points, very short, dredge 4 points, shorter, the thinner scoring of hair is higher.
Result is as shown in table 5.
Table 5 weaned piglets and the comparison of colour of skin hair condition
Note: the different letter representation significant differences of same column shoulder motes (P<0.05), the shoulder motes identical expression there was no significant difference of letter (P>0.05).
Result of the test by table 5 can draw, compare with the second control group with the first control group, test group average daily gain improve respectively 6.4% and 8.1%(P<0.05), material anharmonic ratio decline respectively 4.9% and 5.8%(P<0.05), diarrhea rate reduces respectively 23.5% and 27.8%, in pig manure, copper bar output reduces respectively 26.3% and 29.1%, in pig manure zinc discharge rate reduce respectively 32.3% and 34%(P<0.05), in pig manure iron discharge rate reduce respectively 21.6% and 23.1%(P<0.05), colour of skin scoring improve respectively 34.3% and 40%(P<0.05), hair condition scoring improve respectively 28.6% and 31.4%(P<0.05).Wherein the daily gain of test group, material anharmonic ratio, excrement zinc, excrement iron, colour of skin scoring and the scoring of hair condition are significantly better than the first control group and the second control group (P<0.05), indices there are no significant difference (P>0.05) between the first control group and the second control group.Visible, the daily gain of test group, material anharmonic ratio, excrement zinc, excrement iron, colour of skin scoring and the scoring of hair condition are significantly better than the first control group and the second control group, indices there are no significant difference between the first control group and the second control group.Result shows that yeast metabolism product can promote the synergistic performance of each trace element, improves micro-utilization rate; The feed (the piglet special feed that embodiment 12 provides) that the biological feedstuff composition that the embodiment of the present invention 2 provides makes can significantly improve average daily gain, significantly reduces the content of trace element in material anharmonic ratio, diarrhea rate and excrement, can obviously improve the colour of skin and hair condition.
Embodiment 18 feeding effect tests
Test is divided into test group and control group, and control group comprises the first control group and the second control group.The piglet special feed that test group adopts embodiment 13 to provide, the first control group adopts the first control feed to feed, and the second control group adopts the second control feed to feed.
The preparation method of the first control feed is: organic trace element is in trace element, get cupric glycinate (in copper 10kg), ferrous fumarate (in iron 30kg), zinc lactate (in zinc 32.5kg), manganese methionine (in manganese 9kg), yeast selenium (in selenium 100g) and nicotinate chromium (in chromium 60g), mix, obtain premix semi-finished product.Get the premix semi-finished product 400kg making, antioxidant dibutyl hydroxy toluene 200g, supplies 1000kg with zeolite powder, mixes, and obtains premix.The basal diet 996kg that getting this premix 4kg and embodiment 13 provides mixes, and to obtain final product.Compared with the piglet special feed providing with embodiment 13, in the first control feed, do not add yeast metabolism product.
The second control feed is that inorganic microelement premix and basal diet mix, in the piglet special feed that its trace element, antioxidant, carrier, basal diet and content provide with embodiment 13, corresponding component and content are consistent, difference is that the trace element that the second control feed adopts is inorganic microelement, and does not add yeast metabolism product.
The Rongchang Pig piglet 360 (male and females half and half) of choosing average weight 13kg carries out, and according to body weight, principle that sex is consistent, is divided at random 3 groups, and every processing 6 repeats, 20 pigs of each repetition.Experimental group and control group are take NRC(2012) pig nutritional requirements is standard, take corn-soybean meal diet as basal diet.Test piglet body weight reaches 30kg and finishes to use.Before test, thoroughly clean column home, use 2%NaOH solution to rinse colony house, then the formalin of use and potassium permanganate are pressed stifling a few hours of 2:1, and ventilation cleaning, after one week, starts test.Before formal test starts, carry out the prerun of 7 days, between preliminary trial period, test pig is carried out to expelling parasite, epidemic prevention.Just trying every day phase 8:00 and 17:00 timing throw to powder-material, free choice feeding and drinking-water, observe, record pig health status, records full phase feed intake.Experimental period,, testing index comprised: copper, zinc and iron content in average daily gain, material anharmonic ratio, diarrhea rate, pig manure, colour of skin scoring, the scoring of hair condition.
Index calculating method:
Average daily gain=(off-test body weight-initial body weight)/test number of days
Material anharmonic ratio=average daily material consumption (kg/d/ head)/average daily gain (kg/d/ head)
Total number * 100% of diarrhea rate=diarrhea rate pig's head number/pig
Colour of skin methods of marking: general 1 point, more ruddy 2 points, ruddy 3 points, very ruddy 4 points, the more ruddy scoring of the colour of skin is higher.
Hair condition methods of marking: long and close 1 point, long, close 2 points, shorter, dredge 3 points, very short, dredge 4 points, shorter, the thinner scoring of hair is higher.
Result is as shown in table 6.
Table 6 weaned piglets and the comparison of colour of skin hair condition
Note: the different letter representation significant differences of same column shoulder motes (P<0.05), the shoulder motes identical expression there was no significant difference of letter (P>0.05).
Result of the test by table 6 shows, compare with the second control group with the first control group, test group average daily gain improve respectively 6.5% and 7.2%(P<0.05), material anharmonic ratio decline respectively 4.7% and 5.6%(P<0.05), diarrhea rate reduces respectively 21.1% and 25%, in pig manure, copper bar output reduces respectively 21.9% and 27.1%, in pig manure zinc discharge rate reduce respectively 30.2% and 33%(P<0.05), in pig manure iron discharge rate reduce respectively 19.5% and 21%(P<0.05), colour of skin scoring improves respectively 33.3% and 39.4% (P<0.05), hair condition scoring improve respectively 23.5% and 29.4%(P<0.05).Wherein scoring is significantly better than comparing (P<0.05) with the first control group with the second control group with hair condition for the daily gain of test group, material anharmonic ratio, excrement zinc, excrement iron, colour of skin scoring, compare an indices there are no significant difference (P>0.05) with the second control group with the first control group.Visible, the daily gain of test group, material anharmonic ratio, excrement zinc, excrement iron, colour of skin scoring and the scoring of hair condition are significantly better than the first control group and the second control group, indices there are no significant difference between the first control group and the second control group.Result shows that yeast metabolism product can promote the synergistic performance of each trace element, improves micro-utilization rate; The feed (the piglet special feed that embodiment 13 provides) that the biological feedstuff composition that the embodiment of the present invention 3 provides makes can significantly improve average daily gain, significantly reduces the content of trace element in material anharmonic ratio, diarrhea rate and excrement, can obviously improve the colour of skin and hair condition.
Get the piglet special feed that embodiment 14,15 makes and carry out feeding effect test, the result of test effect and embodiment 11,12 or 13 is close.Result shows that yeast metabolism product can promote the synergistic performance of each trace element, improves micro-utilization rate; The feed (the piglet special feed that embodiment 14,15 provides) that the biological feedstuff composition that the embodiment of the present invention 4,5 provides makes can significantly improve average daily gain, significantly reduce the content of trace element in material anharmonic ratio, diarrhea rate and excrement, can obviously improve the colour of skin and hair condition.
As can be seen here, yeast metabolism product can promote the synergistic performance of each trace element, improves micro-utilization rate; The feed good palatability, the absorptivity that adopt biological feedstuff composition provided by the invention to make are high, without potential heavy metal risk, safety and environmental protection, can significantly improve average daily gain, the content that significantly reduces trace element in material anharmonic ratio, diarrhea rate and excrement, has improved the production performance of piglet, can improve intestinal health, improve food conversion ratio, can obviously improve the colour of skin and hair condition.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. a biological feedstuff composition, is characterized in that, includes organic microelement and yeast metabolism product;
Described organic trace element comprises cupric glycinate, ferrous fumarate, zinc lactate, manganese methionine, yeast selenium and nicotinate chromium;
The preparation method of described yeast metabolism product is: get yeast culture and pulverize, take water as solvent extraction, obtain extract; Described extract, through concentrated, centrifugal, is collected supernatant; Described supernatant is purified, concentrated, dry, to obtain final product.
2. biological feedstuff composition according to claim 1, it is characterized in that, described organic trace element is in trace element, and the mass ratio of described cupric glycinate, described ferrous fumarate, described zinc lactate, described manganese methionine, described yeast selenium, described nicotinate chromium and described yeast metabolism product is (5～12.5): (15～40): (22.5～55): (4～10): (0.075～0.13): (0.04～0.09): (40～60).
3. the application of biological feedstuff composition as claimed in claim 1 or 2 in preparation piglet compound premix special or piglet special feed.
4. a piglet compound premix special, is characterized in that, comprises carrier, antioxidant and biological feedstuff composition as claimed in claim 1 or 2.
5. piglet compound premix special according to claim 4, is characterized in that, the quality percentage composition that described biological feedstuff composition accounts for described piglet compound premix special is 28%～65%.
6. piglet compound premix special according to claim 5, is characterized in that, the quality percentage composition that described antioxidant accounts for described piglet compound premix special is 0.02%～0.06%, and surplus is carrier.
7. piglet compound premix special according to claim 4, is characterized in that, described antioxidant is a kind of in ethoxyquinoline or dibutyl hydroxy toluene or both mixtures.
8. piglet compound premix special according to claim 4, is characterized in that, described carrier is a kind of of stone flour, bentonite or zeolite powder or the mixture more than both.
9. a piglet special feed, is characterized in that, comprises basal diet and the piglet compound premix special as described in any one in claim 4 to 8.
10. piglet special feed according to claim 9, is characterized in that, the quality percentage composition that described piglet compound premix special accounts for described piglet special feed is 0.2%～0.4%.
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Denomination of invention: Organic microelement-containing biological feed composition, application thereof, and special compound premix and special feed of piglets Effective date of registration: 20160728 Granted publication date: 20160316 Pledgee: Agricultural Bank of China, Limited by Share Ltd, Chengdu, Jinjiang branch Pledgor: Sichuan Animtech Feed Co., Ltd. Registration number: 2016510000038 |
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