CN103709262A - Extraction method for high purity auricularia auricula polysaccharide - Google Patents
Extraction method for high purity auricularia auricula polysaccharide Download PDFInfo
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- CN103709262A CN103709262A CN201310630767.8A CN201310630767A CN103709262A CN 103709262 A CN103709262 A CN 103709262A CN 201310630767 A CN201310630767 A CN 201310630767A CN 103709262 A CN103709262 A CN 103709262A
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Abstract
Belonging to the technical field of natural product extraction, the invention relates to an extraction method for high purity auricularia auricula polysaccharide. The method includes the steps of: taking auricularia auricula and drying it in the sun, then grinding it into powder; adding an ethanol water solution into the powder, conducting extraction to remove solids to obtain a extracted solution, and then subjecting the extracted solution to pressure reduction recovery, thus obtain a thick paste; cooling the thick paste, then adding water into the thick paste to produce precipitates, and removing the supernatant, thus obtaining the precipitates; adding ethyl acetate and water into the precipitates, conducting extraction, abandoning the extract phase, collecting an aqueous phase, performing pressure reduced concentration to obtain a concentrated solution, and then carrying out spray drying, thus obtaining the high purity auricularia auricula polysaccharide. The extraction method for auricularia auricula polysaccharide provided by the invention has the advantages of mild operation conditions, easily available raw materials, simple process, high yield, and good purity.
Description
Technical field
The present invention relates to a kind of extracting method of Auricularia polysaccharide, particularly relate to a kind of extracting method of Auricularia polysaccharide of higher degree, belong to natural product extracting technique field.
Background technology
Generally refer to from the polysaccharide composition in large edible or medicinal fungi.The important physiological function of fungus polysaccharide includes: (1) produces by activated macrophage, stimulation antibody, improves human immunological competence; (2) there is strong anti-tumor activity, cancer cells is had to very strong restraint; (3) other effect: anti-ageing, hypoglycemic, reducing blood-fat, antithrombotic, protect the liver, promote protein and nucleic acid is synthetic, the hemopoietic function that increases white corpuscle-radioresistance, antiulcer agent, anti-inflammatory, anticoagulation, enhancing marrow.The food (medicine) of research report had four classes with granulose in recent years: mixed polysaccharide (Heteropolysaccharide), mannosans (Mannan), dextran (Glucan), glycoprotein (Glycoprotein) and polysaccharide peptide (Polysaccharide peptide).
Auricularia auriculajudae, another name black fungus, light auricularia auriculajudae, be Mycophyta Basidiomycetes Auriculariaceae Auricularia plant, is the Jelly fungi of the dietotherapeutic of China's preciousness, general single raw or all living creatures.At present cultivation take linden with Bag Material as main.Black fungus is traditional edible medicinal fungus that Chinese people like, not only meat is fine and smooth, crisp smooth mouthful, and nutritious, contain protein, fat, carbohydrate and multivitamin and inorganic salt, be indispensable black-food during people live, be called as " meat or fish in element ", have the title of " black national treasure ".
The extracting method of Auricularia polysaccharide mainly contains: hot water extraction, diluted alkaline extraction, zymohydrolysis extracting method etc.Although these methods are comparatively easy, the purity of the polysaccharide obtaining is not high generally in 10%～20% left and right.
Summary of the invention
The object of the invention is: a kind of extracting method of high purity Auricularia polysaccharide is provided, has adopted following technical scheme:
An extracting method for highly purified Auricularia polysaccharide, comprises the steps:
The 1st step, by weight, gets 50～70 parts, auricularia auriculajudae, after drying, and grind into powder;
The 2nd step, in powder, add the aqueous ethanolic solution of 5～8 times of weight, lixiviate, removes after solids, obtains vat liquor, then vat liquor reclaim under reduced pressure is become to thick paste;
The 3rd step, thick paste is cooled to 2～4 ℃, then in thick paste, adds the water of 200～300 parts, make to produce precipitation, supernatant liquid is removed, obtain precipitation;
The 4th step adds 40～60 parts, 30～50 parts of ethyl acetate and water in precipitation, and extraction, abandons extraction phase, and water is collected, and carries out concentrating under reduced pressure, and after obtaining concentrated solution, spraying is dry, obtains.
Preferably, the mass concentration of the aqueous ethanolic solution in the 2nd step is 50～70%.More excellent is 60%.
Preferably, the temperature of the lixiviate in the 2nd step is 65～75 ℃.
Preferably, the time of the lixiviate in the 2nd step is 2～4 hours.
Preferably, in the 4th step, spray-dired inlet temperature is 150～160 ℃, 70～80 ℃ of air outlet temperatures.
In method provided by the invention, the object of the 1st step is that auricularia auriculajudae is broken, can make active substance in fact be leached better, it is to promote separating out of active substance that the 2nd step adds the effect of ethanol, the effect of the 3rd step is the mode by depositing in water, and active substance is precipitated, and can be separated with other composition, the effect of the 4th step is that the mode by extraction makes some be dissolved in composition in organic phase to be extracted mutually and to take away, improve the purity of polysaccharide.
beneficial effect
The extracting method of Auricularia polysaccharide provided by the invention, operational condition is gentle, and raw material is easy to get, and technique is simple, and yield is high, and purity is good.
Embodiment
Embodiment 1
The 1st step, get auricularia auriculajudae 50Kg, after drying, grind into powder;
The 2nd step, in powder, add the aqueous ethanolic solution of 5 times of weight, lixiviate, removes after solids, obtains vat liquor, then vat liquor reclaim under reduced pressure is become to thick paste;
The 3rd step, thick paste is cooled to 2 ℃, then in thick paste, adds the water of 200Kg, make to produce precipitation, supernatant liquid is removed, obtain precipitation;
The 4th step adds ethyl acetate 30Kg and water 40Kg in precipitation, and extraction, abandons extraction phase, and water is collected, and carries out concentrating under reduced pressure, and after obtaining concentrated solution, spraying is dry, obtains.
The mass concentration of the aqueous ethanolic solution in the 2nd step is that the temperature of the lixiviate in 50%, the 2 step is 65 ℃, and the time of the lixiviate in the 2nd step is 2 hours, and in the 4th step, spray-dired inlet temperature is 150 ℃, 70 ℃ of air outlet temperatures.
With reference to polysaccharide detection method under the glossy ganoderma of recording in 2010 editions one of Chinese Pharmacopoeia or sealwort item, test, the content of the Auricularia polysaccharide obtaining is 32%.
Embodiment 2
The 1st step, get auricularia auriculajudae 70Kg, after drying, grind into powder;
The 2nd step, in powder, add the aqueous ethanolic solution of 8 times of weight, lixiviate, removes after solids, obtains vat liquor, then vat liquor reclaim under reduced pressure is become to thick paste;
The 3rd step, thick paste is cooled to 4 ℃, then in thick paste, adds the water of 300Kg, make to produce precipitation, supernatant liquid is removed, obtain precipitation;
The 4th step adds ethyl acetate 50Kg and water 60Kg in precipitation, and extraction, abandons extraction phase, and water is collected, and carries out concentrating under reduced pressure, and after obtaining concentrated solution, spraying is dry, obtains.
The mass concentration of the aqueous ethanolic solution in the 2nd step is that the temperature of the lixiviate in 70%, the 2 step is 75 ℃, and the time of the lixiviate in the 2nd step is 4 hours, and in the 4th step, spray-dired inlet temperature is 160 ℃, 80 ℃ of air outlet temperatures.
With reference to polysaccharide detection method under the glossy ganoderma of recording in 2010 editions one of Chinese Pharmacopoeia or sealwort item, test, the content of the Auricularia polysaccharide obtaining is 34%.
Embodiment 3
The 1st step, get auricularia auriculajudae 60Kg, after drying, grind into powder;
The 2nd step, in powder, add the aqueous ethanolic solution of 6 times of weight, lixiviate, removes after solids, obtains vat liquor, then vat liquor reclaim under reduced pressure is become to thick paste;
The 3rd step, thick paste is cooled to 3 ℃, then in thick paste, adds the water of 250Kg, make to produce precipitation, supernatant liquid is removed, obtain precipitation;
The 4th step adds ethyl acetate 40Kg and water 50Kg in precipitation, and extraction, abandons extraction phase, and water is collected, and carries out concentrating under reduced pressure, and after obtaining concentrated solution, spraying is dry, obtains.
The mass concentration of the aqueous ethanolic solution in the 2nd step is that the temperature of the lixiviate in 60%, the 2 step is 70 ℃, and the time of the lixiviate in the 2nd step is 3 hours, and in the 4th step, spray-dired inlet temperature is 155 ℃, 74 ℃ of air outlet temperatures.
With reference to polysaccharide detection method under the glossy ganoderma of recording in 2010 editions one of Chinese Pharmacopoeia or sealwort item, test, the content of the Auricularia polysaccharide obtaining is 36%.
Claims (7)
1. an extracting method for highly purified Auricularia polysaccharide, is characterized in that, comprises the steps:
The 1st step, by weight, gets 50～70 parts, auricularia auriculajudae, after drying, and grind into powder;
The 2nd step, in powder, add the aqueous ethanolic solution of 5～8 times of weight, lixiviate, removes after solids, obtains vat liquor, then vat liquor reclaim under reduced pressure is become to thick paste;
The 3rd step, thick paste is cooled to 2～4 ℃, then in thick paste, adds the water of 200～300 parts, make to produce precipitation, supernatant liquid is removed, obtain precipitation;
The 4th step adds 40～60 parts, 30～50 parts of ethyl acetate and water in precipitation, and extraction, abandons extraction phase, and water is collected, and carries out concentrating under reduced pressure, and after obtaining concentrated solution, spraying is dry, obtains.
2. the extracting method of highly purified Auricularia polysaccharide according to claim 1, is characterized in that: the mass concentration of the aqueous ethanolic solution in the 2nd step is 50～70%.
3. the extracting method of highly purified Auricularia polysaccharide according to claim 2, is characterized in that: the mass concentration of described aqueous ethanolic solution is 60%.
4. the extracting method of highly purified Auricularia polysaccharide according to claim 1, is characterized in that: the temperature of the lixiviate in the 2nd described step is 65～75 ℃.
5. the extracting method of highly purified Auricularia polysaccharide according to claim 1, is characterized in that: the time of the lixiviate in the 2nd described step is 2～4 hours.
6. the extracting method of highly purified Auricularia polysaccharide according to claim 1, is characterized in that: in the 4th described step, spray-dired inlet temperature is 150～160 ℃.
7. the extracting method of highly purified Auricularia polysaccharide according to claim 1, is characterized in that: in the 4th described step, spray-dired air outlet temperature is 70～80 ℃.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105273102A (en) * | 2015-11-03 | 2016-01-27 | 广西南宁胜祺安科技开发有限公司 | Method for extracting auricularia auricula polysaccharide from auricularia auricula |
| CN105728431A (en) * | 2016-02-24 | 2016-07-06 | 齐芳 | Cleaning technology for vinasse |
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| CN1580079A (en) * | 2004-05-21 | 2005-02-16 | 大连轻工业学院 | Method for extracting edible tree fungi polysaccharide |
| CN102060934A (en) * | 2010-12-11 | 2011-05-18 | 中国计量学院 | Enzymatic extraction method for auricularia auricula polysaccharides |
| CN102321189A (en) * | 2011-10-24 | 2012-01-18 | 周伯扬 | Comprehensive extraction process for auricularia auricula polysaccharide |
| CN102585032A (en) * | 2012-03-09 | 2012-07-18 | 武汉大学 | Water-soluble polysaccharide extracted from black fungus and preparation method thereof |
| CN102827302A (en) * | 2012-09-07 | 2012-12-19 | 龙海市颖欣农业科技有限公司 | Preparation method of auricularia auricular polysaccharides |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580079A (en) * | 2004-05-21 | 2005-02-16 | 大连轻工业学院 | Method for extracting edible tree fungi polysaccharide |
| CN102060934A (en) * | 2010-12-11 | 2011-05-18 | 中国计量学院 | Enzymatic extraction method for auricularia auricula polysaccharides |
| CN102321189A (en) * | 2011-10-24 | 2012-01-18 | 周伯扬 | Comprehensive extraction process for auricularia auricula polysaccharide |
| CN102585032A (en) * | 2012-03-09 | 2012-07-18 | 武汉大学 | Water-soluble polysaccharide extracted from black fungus and preparation method thereof |
| CN102827302A (en) * | 2012-09-07 | 2012-12-19 | 龙海市颖欣农业科技有限公司 | Preparation method of auricularia auricular polysaccharides |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105273102A (en) * | 2015-11-03 | 2016-01-27 | 广西南宁胜祺安科技开发有限公司 | Method for extracting auricularia auricula polysaccharide from auricularia auricula |
| CN105728431A (en) * | 2016-02-24 | 2016-07-06 | 齐芳 | Cleaning technology for vinasse |
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