CN102766588B - Kitchen waste destructive compound microbial bactericide, its preparation method and application thereof - Google Patents
Kitchen waste destructive compound microbial bactericide, its preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a kitchen waste destructive compound microbial bactericide, which comprises the following components of: by weight, 0.5-2% of lactics (Pediococcus acidilactici), 0.5-1.5% of yeast (Pichia pastoris), 40-50% of rice bran, 40-50% of wheat bran, 1-10% of soybean meal and 1-10% of protein powder, effective content of the lactics is 1.5*106-3.0*106cfu/g and effective content of the yeast is 1.0*106-3.5*106cfu/g. The compound bactericide provided by the invention is composed of two bacterial strains which are simple to ferment, require low cost, both belongs to nontoxic bacterial strains and have obvious aerobic processing effects for kitchen waste. Processing cost is reduced. Therefore, the compound microbial bactericide has great practical and popularization values.
Description
Technical field
The invention belongs to microorganism field, particularly a kind of complex microbial inoculum, also relate to the preparation method and application of this complex microbial inoculum.
Background technology
The changing food waste main component comprises rice and flours food residues, vegetables, vegetable and animals oils, meat bone etc., chemical constitution, starch, Mierocrystalline cellulose, protein, lipid and inorganic salt is arranged.Principal feature is that organic content is abundant, moisture content is high, perishable, and its proterties and smell all can make a very bad impression to environmental health, and easily grow pathogenic micro-organism, the objectionable impurities such as mycotoxin.
Due to cooking culture and the custom of having a dinner party, produce the changing food waste of flood tide every day.The annual changing food waste that produces of Chinese city is not less than 6,000 ten thousand tons.Nutritious changing food waste is valuable renewable resources.But, owing to not yet drawing attention, method of disposal is improper, it has become affects the potentially dangerous of food safety and ecological safety source.Changing food waste has the dual nature of refuse and resource, can be described as typical " having misplaced local resource ".And unprocessed directly raising livestock and poultry can bring harm to HUMAN HEALTH by the accumulation of livestock and poultry vivotoxin, objectionable impurities again, thereby cause the cross infection between people and animals.Contain the toxic substances such as flavacin, benzene in " sewer oil " that also has current illegal retailer to sell, through underground approach, get back to people's dining table, human consumption causes the generation of chronic disease even carcinogenic, and people healthy caused to great harm.
On September 22nd, 2011, Standing Committee of Beijing Municipal People's Congress's review " Domestic Waste In Beijing management rules (draft) ".Flow into the channel of dining table for cutting off sewer oil, draft requires that certain scale is qualified should build rubbish treatment facility on the spot with regard to meal.The water content of changing food waste is high, and easily corrupt, stink is large, easily goes into secondary pollution, the inconvenience of also bringing for its transportation.Therefore, how to carry out the original position harmless treatment of changing food waste to continuous effective fast, become the current social problem demanding prompt solution.In recent years, in the changing food waste Aerobic, because changing food waste has easy corruption, becomes sour, cause the environment for the treatment of media very easily to change, make the microbic activity capacity of decomposition descend, the smell and the treatment effect that produce stench undesirable thereupon.
Existing to changing food waste processing microbial inoculum, mostly adopt the conducts such as genus bacillus, pseudomonas to clear up bacterial strain, because these bacterial classification resistance to acids are not strong, the ability that suppresses the harmful microorganism growth, cause the treatment time short, process filler and add the easy step-down of pH in process at changing food waste, cause the microorganism growth environmental change, capacity of decomposition descends, and treatment effect is undesirable.China's patent application, application number is " 200810223029 ", name is called " a kind of for pretreated composite fungus agent of changing food waste and its preparation method and application ", a kind of composite fungus agent that fatty genus bacillus, Bacillus licheniformis, fermentation single cell bacterium, edaphic bacillus, yeast saccharomyces cerevisiae and candiyeast be bacterial classification of take is disclosed, although this application has certain active effect to the changing food waste aerobic treatment, but due to its fermentation and complicated process of preparation, production cost is higher, and the antagonistic action between every kind of bacterium is more intense, larger for the stability influence of product.This microbial inoculum does not have strong acid resistance in addition, the easy souring of changing food waste, and the treatment time is long, easily produces stink, so process and bring a lot of inconvenience to changing food waste.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide a kind of changing food waste to subdue the type complex microbial inoculum.
Another object of the present invention is to provide a kind of changing food waste to subdue the preparation method of type complex microbial inoculum.
An also purpose of the present invention is to provide a kind of changing food waste to subdue the application of type complex microbial inoculum in decomposing changing food waste.
In order to realize the object of the invention, a kind of changing food waste of the present invention is subdued the type complex microbial inoculum, it comprises the component of following weight percent: milk-acid bacteria (Pediococcus acidilactici) 0.5%-2%, yeast (Pichia pastoris) 0.5%-1.5%, rice bran 40%-50%, wheat bran 40-50%, beans cypress 1%-10%, protein powder 1%-10%; The effective content of described milk-acid bacteria is 1.5 * 10
6-3.0 * 10
6cfu/g, the effective content of described yeast is 1.0 * 10
6-3.5 * 10
6cfu/g.
Preferably, comprise the component of following weight percent: milk-acid bacteria (Pediococcus acidilactici) 1.5%, yeast (Pichia pastoris) 1%, rice bran 46%, wheat bran 45.5%, beans cypress 5%, protein powder 1%; The effective content of described milk-acid bacteria is 3 * 10
6cfu/g, the effective content of described yeast is 3.5 * 10
6cfu/g.Described milk-acid bacteria is preferably the pediococcus acidilactici that preserving number is CGMCC No.5959, and described yeast is preferably Pasteur that preserving number is CGMCC No.5960 than red yeast.
The milk-acid bacteria that described preserving number is CGMCC No.5959 can decomposition glucose and protein, it is transformed into to lactic acid more than 50%, therefore suitablely breeds under sour environment, and there is very strong capacity antacid, can in the environment of pH3, survive, compare other milk-acid bacterias and have more acid resistance.
Described bacterium is spherical shape, and on Liang Ge plane, right angle, alternately division forms the tetrad shape, and general cell is given birth in pairs, and single survivor is rare, does not become catenation.Gram-positive, do not move, and produces acid, amphimicrobian.On the MRS substratum, bacterium colony is little, is white in color.Grower along agar puncture line is thread.The catalase feminine gender, do not produce cytopigment.
The yeast that described preserving number is CGMCC No.5960 has the thermophilic fermentation ability, can be at the temperature of 60 ℃ the matrix such as fast decoupled lactic acid, amino acid, organism, protein, compare other yeast fecundities stronger, this bacterium can coexist with milk-acid bacteria, to promoting milk-acid bacteria increment tool to have very important significance, this bacterium mainly is responsible for the thermophilic fermentation of changing food waste, accelerates rate of decomposition, promotes the increment of effective microbe.Described bacterial classification is spherical bacterium, and on wort agar, bacterium colony is oyster white, tarnish, and there is thin breach at edge.In wort, cultivate, the nutrient solution surface have white and the wrinkle coarse bacterium uncut jade, bacterial sediment is arranged at the end.
On the other hand, the invention provides the preparation method that a kind of changing food waste is subdued the type complex microbial inoculum, it specifically comprises the following steps:
(1) bacterial strain expands numerous cultivation: cultivate respectively described milk-acid bacteria and yeast strain in substratum, obtain milk-acid bacteria and yeast and expand numerous cultivation bacterium liquid;
(2) solid-state bacteria fermentation: the numerous cultivation of above-mentioned expansion bacterium liquid evenly is sprayed on the solid fermentation substratum and carries out the solid fungicide fermentation, and described solid fermentation substratum comprises rice bran, wheat bran, beans cypress and protein powder, uses the film capping, is cultivated.
Substratum described in step (1) is mixed protein peptone in every 1 premium on currency (protease peptone) 1-1.5g, beef extract (beef extract) 0.5-1g, glucose (glucose) 1-2.0g, sodium acetate (sodium acetate) 3-4g, and Trisodium Citrate (ammoni acitrate) 2-5g; Culture condition is temperature 40-42 ℃, cultivates 24-48 hour.
Solid fermentation substratum described in step (2), and stirs to 35-45% with the water ratio of the warm water of 40-60 ℃ regulation and control solid fermentation substratum.
Incubation time is 48-72 hour in step (2), during every 24 hours, stir once.
The envelope-bulk to weight ratio that expands numerous cultivation bacterium liquid and solid fermentation substratum described in step (2) is 1L:15kg-30kg.
Beneficial effect of the present invention:
(1) yeast in composite fungus agent of the present invention utilizes self fermentation capacity, amino acid, and carbohydrate, organism etc. change into effective substance and help propagation and the activity of other effective microbes;
(2) changing food waste composite fungus agent of the present invention can keep active under the environment of pH5.0-6.5, keeps degradation capability more than 98%;
(3) composite fungus agent of the present invention can maintain filler and not change for a long time, and the part biological filler can only maintain the work-ing life of 2-4 month at present, and this microbial inoculum can maintain more than 1 year;
(4) composite fungus agent of the present invention can be under the hot environment of 60 ℃ the aerobic treatment changing food waste, there are resistant to elevated temperatures characteristics;
(5) composite fungus agent of the present invention can effectively suppress distributing of the stinks such as amine, hydrogen sulfide, relies on thalline to eliminate foul odour to the decomposition of corrupt rubbish;
In a word, composite fungus agent of the present invention, by two kinds of strain combinations, ferments easy, and cost is low, all belongs to non-toxic strain, to changing food waste aerobic treatment successful, reduces processing cost, so very large practical and popularizing value is arranged.
The accompanying drawing explanation
Fig. 1 processes 60 days garbage weight variation diagrams in application examples of the present invention.
Embodiment
Following examples are used for the present invention is described, but are not used for limiting the scope of the invention.Without departing from the spirit and substance of the case in the present invention, the modification that the inventive method, step or condition are done or replacement, all belong to protection scope of the present invention.
If do not specialize, the conventional means that in embodiment, technique means used is well known to those skilled in the art.
Embodiment 1
Described milk-acid bacteria and yeast all separate and obtain from swill, the invention discloses a kind of pediococcus acidilactici (Pediococcus acidilactici), and its preservation name is called HBS-RS, preserving number: CGMCC No.5959.The invention also discloses a kind of pichia pastoris (Pichia pastoris), its preservation name is called HBS-Hab, preserving number: CGMCC No.5960.Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica; Preservation date: on April 9th, 2012.
Embodiment 2 changing food wastes are subdued the type complex microbial inoculum
1) slant culture: will under the yeast in embodiment 1 and milk-acid bacteria original strain aseptic condition, be inoculated in respectively on solid medium, cultivate 2 days under 37 ℃ of conditions;
2) be inoculated in liquid nutrient medium under the bacterial classification aseptic condition of first order seed cultivation: by step 1) cultivating, under 42 ℃ of conditions, the 150r/min shaking table is cultivated 1 day, and while finishing to cultivate, yeast and milk-acid bacteria suspension liquid light density OD600 value all reach 4.0;
3) secondary seed is cultivated: the inoculum size that is 10% by the volume ratio of liquid nutrient medium, first order seed is inoculated into respectively in the fermentor tank of 1000L, in fermentor tank, the cumulative volume of nutrient solution is 500L, under 42 ℃ of conditions of yeast and milk-acid bacteria, stirring velocity is 150r/min, air flow is 1:1, cultivates 1 day, makes secondary seed;
Wherein, step 1), 2), yeast and milk-acid bacteria substratum used are proteolytic enzyme peptone (protease peptone) 1g, beef extract (beef extract) 1g, glucose (glucose) 2g, sodium acetate (sodium acetate) 3g, and Trisodium Citrate (ammoni acitrate) 2g.
The formula of substratum step 3) used is by mass percentage: Semen Maydis powder 2.5%, soyflour 1%, peptone 1%, yeast powder 2.5%, K
2hPO
40.5%, MgSO
40.005%, tween 80 1%, sodium-acetate 0.5%, surplus are water, pH6.4.
The fermentation culture process comprises: in initial 12 hours, the interval ventilation, remain on the aerobic conditions fermentation, air flow 1:1, and regulation and control fermentation dissolved oxygen 10%, mixing speed 180r/min, 2 hours mixing chamber intervals, stir 42 ℃ of temperature 2 minutes; Slightly soluble oxygen and anaerobism cultivation stage: after 12 hours, keep fermented liquid upper strata slightly soluble oxygen condition, static cultivation, stir at interval, 4 hours mixing chamber intervals, stirs 42 ℃ of temperature 4 minutes.
Add solid fermentation substratum 7500kg in 500 liters of numerous cultivation bacterium liquid of expansion, described solid fermentation substratum is milk-acid bacteria (Pediococcus acidilactici) 1.5%, yeast (Pichia pastoris) 1%, rice bran (46%), wheat bran (45.5%), beans cypress (5%), protein powder (1%) stir, use the film capping, every stirring in 24 hours, cultivate 72 hours.
Embodiment 3
Expand numerous bacterial classification according to liquid fermentation process in example 2.
Wherein, step 1), 2), yeast and milk-acid bacteria substratum used are proteolytic enzyme peptone (protease peptone) 1.5g, beef extract (beef extract) 0.5g, glucose (glucose) 1g, sodium acetate (sodium acetate) 3g, and Trisodium Citrate (ammoni acitrate) 2g.
The formula of substratum step 3) used is by mass percentage: Semen Maydis powder 1%, soyflour 1%, peptone 1%, yeast powder 1.5%, K
2hPO
40.5%, MgSO
40.005%, tween 80 1%, sodium-acetate 0.5%, surplus are water, pH6.4.
The fermentation culture process comprises: in initial 12 hours, the interval ventilation, remain on the aerobic conditions fermentation, air flow 1:1, and regulation and control fermentation dissolved oxygen 10%, mixing speed 180r/min, 2 hours mixing chamber intervals, stir 41 ℃ of temperature 2 minutes; Slightly soluble oxygen and anaerobism cultivation stage: after 12 hours, keep fermented liquid upper strata slightly soluble oxygen condition, static cultivation, stir at interval, 4 hours mixing chamber intervals, stirs 41 ℃ of temperature 4 minutes.
Expand numerous cultivation bacterium liquid by 500 liters and join (7500kg-8000kg) in the solid fermentation substratum.Described solid fermentation substratum is milk-acid bacteria (Pediococcus acidilactici) 1.5%, yeast (Pichia pastoris) 1%, rice bran (40%), wheat bran (40%), beans cypress (7.5%), protein powder (10%), stir, use the film capping, every stirring in 24 hours, cultivate 48 hours.
Embodiment 4
Expand numerous bacterial classification according to liquid fermentation process in example 2.
Wherein, step 1), 2), yeast and milk-acid bacteria substratum used are proteolytic enzyme peptone (protease peptone) 1g, beef extract (beef extract) 1g, glucose (glucose) 2g, sodium acetate (sodium acetate) 3g, and Trisodium Citrate (ammoni acitrate) 2g.
The formula of substratum step 3) used is by mass percentage: Semen Maydis powder 2.5%, soyflour 2.5%, peptone 2%, yeast powder 2.5%, K
2hPO
41.5%, MgSO
40.005%, tween 80 1%, sodium-acetate 0.5%, surplus are water, pH6.6.
The fermentation culture process comprises: in initial 12 hours, the interval ventilation, remain on the aerobic conditions fermentation, air flow 1:1, and regulation and control fermentation dissolved oxygen 10%, mixing speed 180r/min, 2 hours mixing chamber intervals, stir 40 ℃ of temperature 2 minutes; Slightly soluble oxygen and anaerobism cultivation stage: after 12 hours, keep fermented liquid upper strata slightly soluble oxygen condition, static cultivation, stir at interval, 4 hours mixing chamber intervals, stirs 40 ℃ of temperature 4 minutes.
Expand numerous cultivation bacterium liquid by 500 liters and join 7800kg in the solid fermentation substratum.Described solid fermentation substratum is milk-acid bacteria (Pediococcus acidilactici) 1.5%, yeast (Pichia pastoris) 1%, rice bran (48%), wheat bran (47.5%), beans cypress (1%), protein powder (1%) stir, use the film capping, every stirring in 24 hours, cultivate 66 hours.
Expand numerous bacterial classification according to liquid fermentation process in example 2.
Wherein, step 1), 2), yeast and milk-acid bacteria substratum used are proteolytic enzyme peptone (protease peptone) 1g, beef extract (beef extract) 1g, glucose (glucose) 2g, sodium acetate (sodium acetate) 3g, and Trisodium Citrate (ammoni acitrate) 2g.
The formula of substratum step 3) used is by mass percentage: Semen Maydis powder 1.0%, soyflour 2%, peptone 2.5%, yeast powder 2%, K
2hPO
41.5%, MgSO
40.1%, tween 80 1.5%, sodium-acetate 1.5%, surplus are water, pH6.8.
The fermentation culture process comprises: in initial 12 hours, the interval ventilation, remain on the aerobic conditions fermentation, air flow 1:1.2, and regulation and control fermentation dissolved oxygen 10%, mixing speed 180r/min, 2 hours mixing chamber intervals, stir 42 ℃ of temperature 2 minutes; Slightly soluble oxygen and anaerobism cultivation stage: after 12 hours, keep fermented liquid upper strata slightly soluble oxygen condition, static cultivation, stir at interval, 4 hours mixing chamber intervals, stirs 42 ℃ of temperature 4 minutes.
Expand numerous cultivation bacterium liquid by 500 liters and join 8000kg in the solid fermentation substratum.Described solid fermentation substratum is milk-acid bacteria (Pediococcus acidilactici) 1.5%, yeast (Pichia pastoris) 1%, rice bran (43%), wheat bran (43%), beans cypress (6%), protein powder (5.5%) stir, use the film capping, every stirring in 24 hours, cultivate 72 hours.
Application examples
(1) this time be 30 days experimental period; cultivate flow process according to the solid fungicide of example 2-5 and be made into product; to 4 kinds of product number consecutivelies, be JH1, JH2, JH3, JH4; added up the number of viable of each microbial inoculum every 5 days; following diagram data shows that the JH1 microbial inoculum of example 2 correspondences is best to the absorption protection effect of microorganism, and within 30 days, number of viable remains on 6.5 * 10
6cfu/g, survival rate reaches 85%, and the JH3 microbial inoculum effect of example 3 correspondences is the poorest, and within 30 days, number of viable drops to 4.2 * 10
5cfu/g, survival rate drops to 6.5%.The sequence that draws thus four kinds of cultural methods is JH1>JH2>JH4>JH3, and best training method is example 2.
4 kinds of composite fungus agents of table 1 are processed the number of viable of 30 days
(2) this time be 14 days experimental period, in the aerobic kitchen garbage treater of 1kg treatment capacity, adds continuously at least changing food waste of 540g, the highest 1746g that adds of Dan Tian every day.In the middle of added the rubbish such as chicken bone, Fishbone, dish class, rice, meat.Its method is: described bacterial classification is through liquid fermenting propagation, and field planting makes solid fungicide JH1 in solid filler, and this microbial inoculum is in the addition with 1% is added to kitchen garbage treater.From experiment effect, carrier microbial inoculum quality does not increase, and the non-stimulated smell of decomposition course produces, and it is more than 96% that whole changing food waste is cleared up rate.Contrast before and after adding from changing food waste, after 4 hours, the changing food waste base conditioning is complete, without obviously residual.The material decomposition such as bone are slower, and needs at least decomposed by the above time of two weeks.In this section treating processes, water ratio tends towards stability, and in the handler carrier, temperature is 50 degrees centigrade of left and right, and microorganism growth is good, without the anaerobically fermenting phenomenon.
Table 2 is processed the rubbish total Amount Monitoring result after 14 days
| Experimental period (my god) | Gross weight (g) before processing | Add rubbish total amount (g) | Gross weight (g) after processing |
| 14 | 7000 | 8572 | 7337 |
(3) this time be 28 days experimental period, the aerobic kitchen garbage treater that experimental installation is day output 50kg.Its method is: described bacterial classification is through liquid fermenting propagation, and field planting makes solid fungicide JH1 in solid filler, and this microbial inoculum is in the addition with 1% is added to kitchen garbage treater.It is 450kg that filler adds total amount, 28 days process the changing food waste total amount is 1390kg, last surplus total amount is 380kg, changing food waste is in complete treated state, because a part self filler is also utilized by microbial consumption, and discharge with the heat energy form, cause the surplus total amount lower than adding the filler total amount.
Table 3 is processed the rubbish total Amount Monitoring result after 28 days
| Time (my god) | Add quantity of refuse (kg) | Add filler (kg) | Unspent amount (kg) | Decomposition efficiency |
| 1 | 20 | 400 | ||
| 2 | 20 | |||
| 3 | 20 | |||
| 4 | 30 | 50 | ||
| 5～26 | 50 | |||
| 27 | 100 | |||
| 28 | 100 | |||
| Total amount | 1390 | 450 | 380 | 100％ |
(4) be 60 days this experimental period, the aerobic kitchen garbage treater that experimental installation is day output 1kg.Its method is: make microbial inoculum according to table 4 method, by microbial inoculum, in being added to kitchen garbage treater with 1% addition respectively, the filler gross weight is 7kg.Monitor its changes in weight situation every day.As seen from Figure 1, within 60 days, rubbish accumulative total is added 15kg, and the composite fungus agent processing power of HBS-1 is the strongest, and the rubbish semi-invariant is minimum, and after 60 days, weight is 6.2kg, compares changing food waste and adds total amount, and rubbish decomposes fully.Yet, only adding the microbial inoculum of milk-acid bacteria, treatment effect is slightly poor, and after 60 days, weight is 8.7kg, and processing rate is 89%.Only add saccharomycetic treatment group effect the poorest, after 60 days, weight is 10.5kg, and processing rate is 77%.Thus, can draw, add composite fungus agent and be better than the treatment effect that only adds single bacterium.
The matching method of table 4 different strain addition
The above % unit all is weight percentage.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (8)
1. a changing food waste is subdued the type complex microbial inoculum, and it comprises the component of following weight percent: pediococcus acidilactici (Pediococcus acidilactici) 1.5%, pichia pastoris (Pichia pastoris) 1%, rice bran 46%, wheat bran 45.5%, beans cypress 5%, protein powder 1%; The effective content of described pediococcus acidilactici is 3 * 10
6cfu/g, the effective content of described pichia pastoris is 3.5 * 10
6cfu/g; Described pediococcus acidilactici is the pediococcus acidilactici that preserving number is CGMCCNo.5959, and described pichia pastoris is the pichia pastoris that preserving number is CGMCCNo.5960.
2. prepare the method for composite fungus agent as claimed in claim 1, it specifically comprises the following steps:
(1) bacterial strain expands numerous cultivation: cultivate respectively described pediococcus acidilactici and pichia pastoris bacterial strain in substratum, obtain pediococcus acidilactici and pichia pastoris and expand numerous cultivation bacterium liquid;
(2) solid-state bacteria fermentation: the numerous cultivation of above-mentioned expansion bacterium liquid evenly is sprayed on the solid fermentation substratum and carries out the solid fungicide fermentation, and described solid fermentation substratum comprises rice bran, wheat bran, beans cypress and protein powder, uses the film capping, is cultivated.
3. method as claimed in claim 2, is characterized in that, substratum described in step (1) is mixed protein peptone 1-1.5g in every 1 premium on currency, beef extract 0.5-1g, glucose 1-2.0g, sodium acetate 3-4g, and Trisodium Citrate 2-5g.
4. method as claimed in claim 2, is characterized in that, described in step (1), culture condition is temperature 40-42 ℃, and incubation time is 24-48 hour.
5. method as claimed in claim 2, is characterized in that, solid fermentation substratum described in step (2), and stirs to 35%-45% with the water ratio of the warm water of 40 ℃-60 ℃ regulation and control solid fermentation substratum.
6. method as claimed in claim 2, is characterized in that, incubation time is 48-72 hour in step (2), during every 24 hours, stir once.
7. method as claimed in claim 2, is characterized in that, the envelope-bulk to weight ratio that expands numerous cultivation bacterium liquid and solid fermentation substratum described in step (2) is 1L:15kg-30kg.
8. the application of the described composite fungus agent of claim 1 in decomposing changing food waste.
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| CN103771912B (en) * | 2013-12-31 | 2016-02-17 | 成都恒润高新科技有限公司 | The working method of kitchen castoff and processed products thereof are used as the application of fertilizer |
| CN108277174B (en) * | 2018-01-18 | 2021-09-14 | 轻工业环境保护研究所 | Complex microbial inoculant for aquaculture and application thereof |
| CN109081709B (en) * | 2018-07-08 | 2021-05-14 | 张晓敏 | Urban domestic garbage treatment method |
| CN110813989A (en) * | 2019-11-09 | 2020-02-21 | 浙江浦江加百列生态科技有限公司 | Rapid directional humification fermentation method for household organic garbage |
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