CN108575521A

CN108575521A - A kind of method of Ling Yaoxing and red bayberry interplanting

Info

Publication number: CN108575521A
Application number: CN201810543341.1A
Authority: CN
Inventors: 覃丽青; 黄妙姑; 黄谋勇; 其他发明人请求不公开姓名
Original assignee: Guangxi Wave Tea Ltd By Share Ltd
Current assignee: Guangxi Wave Tea Ltd By Share Ltd
Priority date: 2018-05-31
Filing date: 2018-05-31
Publication date: 2018-09-28

Abstract

The present invention provides a kind of method of Ling Yaoxing and red bayberry interplanting, is related to planting technology field, the method that Ling Yaoxing of the invention is interplanted with red bayberry includes the following steps：(1) readjustment of field plot, the cultivation of (2) pekoe tea seedling, (3) plantation pekoe tea, the pretreatment of (4) red bayberry seedling, (5) interplanting red bayberry seedling, (6) water and fertilizer management and (7) pekoe tea harvest pre-treatment；Red bayberry meets with pekoe tea phasic property, the pest and disease damage not infected mutually, but the growth of pekoe tea can be that red bayberry drives away certain mosquito；The leaf and the ripe Waxberry fruit fallen naturally that red bayberry falls will not have an adverse effect to the growth of tea plant, nutrition can be provided instead, the Ridged plant of pekoe tea, the draining for being conducive to red bayberry is ventilative, to red bayberry bloom and result plays beneficial effect, therefore, red bayberry is mutually promoted with pekoe tea, not only improves the utilization rate in soil, but also can also form symbiosis, respective yield and quality is improved, forest interplanting is suitble to.

Description

A kind of method of Ling Yaoxing and red bayberry interplanting

【Technical field】

The present invention relates to planting technology fields, and in particular to a kind of method of Ling Yaoxing and red bayberry interplanting.

【Background technology】

Ling Yaoxing, original name raw white tea are gained the name due to its blade back covers with pekoe, and main product is curled up in Lingyun County four seasons cloud and mist Cenwanglaoshan, one band of Qinglongshan.Advantageous natural environment, makes that Ling Yaoxing is emerald green with color, milli is more, aromatic, taste is dense, resistance to Five big characteristics are steeped as the rising star in Chinese famous tea.It is high-quality in Ling Yaoxing tree kind system Guangxi District, fit that property processed is wide, Wei Yiguo Family's grade place breeding.The sturdy more millis of the kind bud-leaf, currently, in Guangxi cultivated area more than 300,000 mu.

Mei Yang is semi-tropical fruit tree originating in China, and Economical cultivation is concentrated mainly on the Zhejiang, Jiangsu, good fortune of southeastern coast Build, Guangdong, Jiangxi, Anhui, Hunan and Guizhou etc., Waxberry fruit unique flavor, fruit colour is bright-coloured, and juice is more, sweet and sour palatability, Nutrition and economic value are higher.

It is general in existing Tea planting and red bayberry planting process to be all not carried out normative management, such as a large amount of applications Other artificial measures such as pesticide, chemical fertilizer, herbicide, cause the flavor of product to decline, quality reduction, and the health of consumer is endangered The various problems such as evil.

In addition, there is problems in Tea planting：1) single plantation tealeaves make tea tree for a long time without shaping and Protection, illumination is too strong to cause tea tree metabolism technical ability to slow down, and reduce photosynthetic efficiency, while also reducing the resistance of tea tree, The normal growth for influencing tea tree is deteriorated so as to cause the low output and tea leaf quality in tea place；2) tea place of single cropping tea tree holds Easily there is freeze injury and pest and disease damage, adaptability and fast-growing character are poor, and forest land utilization rate is not high, and the unit area output value is relatively low；3) tea place In the ranks vegetation coverage is insufficient, be easy to cause rain drop erosion, reduces the organic matter of soil, causes the soil erosion in tea place；4) tea The Se content of leaf is insufficient, and with the improvement of living standards, the demand of people's selenium is increasing；Therefore, how tealeaves is improved Yield and kind planting pollution-free and the high tealeaves of selenium content is the current technical issues that need to address.

【Invention content】

In view of this, the purpose of the present invention is to provide a kind of method of Ling Yaoxing and red bayberry interplanting, the present invention is logical It crosses and uses three-dimensional planting mode using red bayberry and difference in height, the required intensity of illumination difference of Ling Yaoxing, will reach the clouds Pekoe tea carries out set plantation with red bayberry and plants, and plants red bayberry in pekoe tea place, can improve tea place ecological environment, promotes tea place ecology The benign cycle of system, tea tree also have facilitation to the growth of red bayberry, realize win-win.

In order to solve the above-mentioned technical problem, present invention employs the following technical solutions：

A kind of method of Ling Yaoxing and red bayberry interplanting, includes the following steps：

(1) readjustment of field plot：It is the soil of 18-30% as pre- kind of ground to select the content of organic matter, is dug to pre- kind of ground, Applied amount according to 95-135kg/ mu after soil deep tillage 55-65cm is applied into disinfectant powder to pre- kind of ground bottom, irrigation is to turning over Bottom is seeped water, and is applied biogas residue after being exposed to the sun 2-3 days, is covered using soil is ploughed deeply, and is applied farm manure, is carefully harrowed, by 15-20 days Curing, then through plough deeply plough after addition soil conditioner soil pH value is adjusted to 4.8-6.0, obtain plantation plot；

(2) cultivation of pekoe tea seedling：1. explant pre-processes：Clip passes through girdling root newly long pekoe tea tender shoots branch For explant, explant is cut into the branch of 5-7cm, 12-16s is impregnated with 75% alcohol, then impregnates 30- with garlic juice 50min is constantly stirred with glass bar during immersion, is rinsed explant 5-7 times with sterile water after the completion of immersion, then will Explant, which is placed on superclean bench, waits for its surface moisture natural air drying, cuts off the blade of explant and old notch, then cuts 8-10min is handled at 2 axillary bud stem sections of 1.5-2cm bands, then with 2% sodium hypochlorite, aseptic water washing 3-5 times is blotted with filter paper Stem segment with axillary bud, the explant for obtaining tissue cultures are spare；2. the cultivation of culture medium：A. Fiber differentiation：By the explant of acquisition It is first inoculated on inducing culture, cultivates 35-40 days, be 1 by dark and light application time ratio:8 carry out Fiber differentiation, callus induction Tissue differentiation forms adventitious bud；The inducing culture contains MS culture medium+1.5mg/L KT+0.5mg/L 6-BA+0.1mg/L IBA+200mg/L Ca(No₃)；B. shoot proliferation culture：Adventitious bud is transferred on proliferated culture medium and is cultivated 35-40 days, is obtained Regenerated plant；The ingredient of the proliferated culture medium is：MS+1.0mg/L KT+0.2mg/L 6-BA+0.1mg/L IBA+200mg/L Ca(No₃)；C. culture of rootage：The Regenerated plant that Multiplying culture obtains is inoculated into root media and carries out culture of rootage, cultivates 15- Rooted seedling is obtained after 20 days, rooted seedling, which is then gone to seeding room, carries out hardening；The ingredient of the root media is：MS+ 1.0mg/L ABT+0.2mg/L IBA；3. hardening：By the pekoe tea shoot of process tissue cultures by being taken out in culture medium, with originally Water cleans the culture medium of seedling root, is impregnated 10-20 minutes with the carbendazim or Bravo solution that dilute 1000 times, is then planted It plants and is mixed in the compost seed plate of peat soil, garden mould and perlite and sprays root water, cover upper plastic film on plant upper layer Water conservation, holding humidity are 75-90%, and 23-26 DEG C of temperature can be obtained pekoe tea seedling after cultivating 20-30 days；

(3) pekoe tea is planted：The organic fertilizer for applying 1200-1800kg per acre, in plantation field according to 10-15cm × 150- The seeding row spacing of 180cm opens up kind of a planting ditch, and the depth × width for planting planting ditch is 40-45cm × 60-65cm, and pekoe tea seedling is put into plantation It is colonized in ditch, Nutrition Soil is used in combination to fill, filled to the thickness of Nutrition Soil to carry out carrying Miao Shugen when 20-30cm, then proceed to use Nutrition Soil is filled, until filling up kind of a plant hole, root water is irrigated after the completion of plantation；The Nutrition Soil is by rape cake, decomposed people Animal manure and rice bran are 3-5 according to mass ratio:1-2:After 1-3 mixing, it is put into frying 5-10 minutes in marmite and is made；

(4) red bayberry seedling pre-processes：Height is selected in 65-90cm, growth period and the pekoe tea same period or is longer than the excellent of pekoe tea Matter red bayberry seedling carries out red bayberry seedling to be stained with root processing, specially：Red bayberry seedling root is soaked in the brine of 13-18%, 5- is impregnated It is taken out after 9 minutes, is impregnated 20-35 minutes in Fungicidal nutritive liquid, yellow mud is then stained in its root, you can；

(5) red bayberry seedling is interplanted：25-30 plants of red bayberry seedling is interplanted in tea place per acre, is interplanted in pekoe tea row, apart from pekoe tea tree 60cm or more, 10-12h first carries out watering wetting to planting pit before planting, and root water is irrigated after the completion of plantation；

25-30 plants or so of red bayberry is interplanted in tea place per acre, interplants in tea row, is due to red bayberry from tea tree 60cm or more Tree crown is closeer, larger, should not plant too close, and interplanting red bayberry influences tea growth too much, and above-mentioned parameter is that applicant constantly tests The Optimum Planting Density gone out.

(6) water and fertilizer management：Pouring fertilising irregularly is carried out to pekoe tea and red bayberry after interplanting, using with sterilizing function Liquid Fertilizer pour applying, to keep the relative humidity in plot for 25-30%；

(7) pekoe tea harvests pre-treatment：It is harvested in the previous moon in pekoe tea, the girdling on each major branch of pekoe tea tree Go out width to be 0.5-2.5cm and cut off the girdling mouth of major branch bast, selection is without the rainy day gas Selenium-rich nutrient solution smearing ring Scarfing smeared primary, smearing 3-5 times altogether every 3-5 days.

In the present invention, further, the disinfectant powder in the step (1) is made of the raw material of following parts by weight：Calcium oxide 10-20 parts, 5-10 parts of bur beggar-ticks extract, 5-12 parts of shrubby sophora extract, 3-8 parts of licorice, 4-9 parts of ginger peel extract, 4-8 parts of 3-8 parts of aloe extract and pearl lotus extract.

In the present invention, further, the Fungicidal nutritive liquid in the step (4) is made of the raw material of following parts by weight：It is secondary 1-3 parts of sodium chlorate, 2-4 parts of garlic oil, 2-4 parts of sodium chloride, 2-4 parts of forchlorfenuron, 2-4 parts of methyl α-naphthyl acetate, witchweed lactone 1-3 Part, 1-2 parts of compound sodium nitrophenolate, 4-6 parts of brown sugar and appropriate amount of water.

In the present invention, further, the organic fertilizer in the step (3) is made of the raw material of following parts by weight：Straw powder 20-30 parts, 18-22 parts of vinasse, 15-20 parts of mushroom dreg, 3-8 parts of pork liver powder, 5-10 parts of malt, 6-12 parts of coconut shell flour, weathering 1-5 parts of 5-10 parts of coal and EM bacterium.

In the present invention, further, the Liquid Fertilizer in the step (6) is made of the raw material of following parts by weight：Biogas slurry 10- 20 parts, 3-8 parts of chitosan, 4-9 parts of garlic oil, 4-9 parts of Folium Isatidis extract, 3-8 parts of fulvic acid, 3-8 parts of heteroauxin potassium, urine 320-450 parts of 8-16 parts plain, 5-10 parts of mulberry leaf ferment, 2-4 parts of fermenting agent and water.

In the present invention, further, it is 3-5 that the fermenting agent, which is by mass ratio,:2-3:1:3 saccharomycete, xanthoma Abrasive net-balloon for stomach bacterium, aspergillus niger, filamentous fungus T. reesei mixing composition.

In the present invention, further, the Selenium-rich nutrient solution in the step (7) is made of the raw material of following parts by weight：Have 1-2 parts of machine selenium, 10-15 parts of sodium carboxymethylcellulose, 2-4 parts of humic acid, 0.5-0.8 parts of Tea Saponin, 1-2 parts of potassium nitrate, chlorination 1000-1200 parts of 1-3 parts of calcium and distilled water；The applying amount of Selenium-rich nutrient solution is 80-120g/ plants.

In the present invention, further, the disinfectant powder is made of the raw material of following parts by weight：15 parts of calcium oxide, bur beggar-ticks 7 parts of extract, 9 parts of shrubby sophora extract, 6 parts of licorice, 7 parts of ginger peel extract, 6 parts of aloe extract and the extraction of pearl lotus 7 parts of object.

In the present invention, further, what the Organic Selenium obtained by the following method：By weight by 12-22 parts of sub- selenium The speed that the ascorbic acid solution low whipping speed that sour sodium is 10-15% with 200-250 parts of mass concentrations is 100-200r/min Under be stirred, then be added 15-30 parts of propolis, continue to be stirred to react 20-30min to get the Organic Selenium.

In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：

1. the present invention uses vertical by using difference in height, the required intensity of illumination difference of red bayberry and Ling Yaoxing Ling Yaoxing and red bayberry are carried out set plantation and planted, planted red bayberry in pekoe tea place, can improve tea place ecology by body cultivation mode Environment promotes the benign cycle of Tea Garden Ecosystem, shades by using strawberry tree, to tea tree play high temperature-proof arid, Prevent that the effect of strong sunshine, red bayberry meet with pekoe tea phasic property, the pest and disease damage not infected mutually, but the growth of pekoe tea can be poplar Plum drives away certain mosquito；Red bayberry is deciduous fruit tree, and the leaf and the ripe Waxberry fruit fallen naturally fallen will not be to windproof The growth of plant has an adverse effect, and can provide nutrition instead, and the Ridged plant of pekoe tea, the draining for being conducive to red bayberry is ventilative, To red bayberry bloom and result plays beneficial effect, therefore, red bayberry is mutually promoted with pekoe tea, not only increases the profit in soil With rate, and symbiosis can also be formed, improve respective yield and quality, be suitble to forest interplanting.

2. the present invention is different from general planting, site preparation is carried out first before planting, by being spilled into applicant on plot certainly The disinfectant powder of system, can kill be unfavorable in soil red bayberry and pekoe tea growth harmful pathogenic bacteria, the disinfectant powder by calcium oxide, Bur beggar-ticks extract, shrubby sophora extract, licorice, ginger peel extract, aloe extract and pearl lotus extract composition, match In addition to calcium oxide is inorganic matter in side, other is all organic matter extract, wherein pearl lotus extract contains eriodictyol, dihydro The careless element of Quercetin, Kaempferol, Dihydrokaempferol, naringenin, Quercetin, art rhinoceros, eriodictyonone, apiolin, chrysoeriol Isoreactivity ingredient has stronger inhibitory activity to all kinds of germs, and bur beggar-ticks, kuh-seng, ginger peel and aloe are in broad-spectrum sterilization Medicine ingredient, extract can safely and effectively kill fungi, and above-mentioned organic matter extract is used in combination, can kill in soil Most of germ passes through other than active ingredient itself can kill pathogenic microorganisms and play the role of antivirus in the later stage Soil turn over bury, stack retting the effects that can also be decomposed into organic matter, not can cause environmental pollution.

3. the present invention carries out it before red bayberry planting seedlings to be stained with root processing, can be killed by the immersion of light salt brine first Most of bacterium on seedling root softens cell wall so that follow-up Fungicidal nutritive liquid can more preferably penetrate into wherein, light salt brine Concentration control between 13-18%, excessive concentration will not be caused and generate root dehydration, burn seedlings phenomenon, sterilization of the invention Nutrient solution is to use sodium hypochlorite, garlic oil, sodium chloride, forchlorfenuron, methyl α-naphthyl acetate, witchweed lactone, compound sodium nitrophenolate and red sand Sugar composition, wherein it is pest-resistant that sodium hypochlorite, garlic oil and sodium chloride play the role of sterilization, prevents field residual germ and external worm Erosion of the evil for sapling also supplements the supply of nutrient in transplanting soil, and forchlorfenuron is mitogen, is assisted with auxin methyl α-naphthyl acetate Same-action ensures that forchlorfenuron gives full play to its effect in the case where there is auxin, and methyl α-naphthyl acetate can regulate and control plant vasular group The differentiation knitted, fertility, seed are sprouted, and witchweed lactone can reduce the translocation of auxin to reduce the generation of branch, The utilization rate of auxin is increased, humidification effect, in conjunction with growth regulator compound sodium nitrophenolate, compound sodium nitrophenolate can connect with plant It can penetrate into plant rapidly after touch, the plasm of cell is promoted to flow, improve cell viability, drive the factor of permeable pressure head It penetrates into plant rapidly；It both can be while red as the nutritional agents during red bayberry seedling early growth in addition, brown sugar is added Granulated sugar also acts as the effect of bonding, so that nutrient solution is adhered on the root system of seedling new life, nutrient solution is avoided to be lost in, and enhances effect of taking root Fruit, the present invention can form one layer of nutrient film after being impregnated to root, prevent the nutriment for being adsorbed on root surface to be lost in, while can To be effectively promoted root growth, promote the division and differentiation of plant cell, increases the resistance of seedling, and red bayberry can be accelerated The growth of seedling.

4. the pekoe tea shoot of the present invention is independently cultivated by applicant, pekoe tea is quickly bred by tissue cultures Seedling carries out sterilization, then in the MS culture mediums matched containing hormon to explant first before carrying out tissue cultures Upper evoked callus, by the culture of inducing culture, proliferated culture medium and root media, each culture medium provides explant The body factor required during strong plantlets and rootage, is capable of providing the good condition taken root, and promotes seedling health healthy and strong, obtains China fir again Raw seedling, cultural method convenient material drawing, at low cost, easy to operate, reproductive speed is fast, and breeding coefficient is high, has good economy Benefit and ecological benefits, are worthy to be popularized.

5. the present invention is harvested in pekoe tea and handled pekoe tea using Selenium-rich nutrient solution the previous moon, selenium-rich can be obtained Pekoe tea, used Selenium-rich nutrient solution are by Organic Selenium, sodium carboxymethylcellulose, humic acid, Tea Saponin, potassium nitrate, chlorination Calcium and distilled water configure, and wherein Organic Selenium is formulated by sodium selenite, ascorbic acid and xylitol, compared to common Sodium selenite, more easily transport and absorbed within plant tissue；Sodium carboxymethylcellulose is excipient and film forming agent, can be with Make nutrient solution that there is certain viscosity, reduce mobility, convenient for smearing, moreover it is possible to form layer protecting film after smearing, prevent outer Infringement of the boundary germ to girdling mouth；Tea Saponin can make the system of Selenium-rich nutrient solution more stable, after smearing, can improve this selenium-rich nutritive Liquid plays the synergistic effect to Selenium-rich nutrient solution in the adhesive force and penetration of pekoe tea girdling discharge surface, and Tea Saponin also has Certain insecticidal activity can prevent the invasion of disease；Potassium nitrate, humic acid and calcium chloride are thus needed for growth phase pekoe tea Macronutrient, science addition, can supplement a great number of elements needed for pekoe tea, enhance the energy of tree vigo(u)r and the conveying nutrition of each position Power, so as to accelerate the absorption of Organic Selenium.Therefore, formula is scientific and reasonable for this Selenium-rich nutrient solution, the work(of each raw material in formula Can effect be combined with each other, play the role of synergy, can greatly improve pekoe tea in this stage to the absorption of selenium element level, To play better selenium-rich effect.

【Specific implementation mode】

The following examples can help those skilled in the art that the present invention is more fully understood, but cannot be with any Mode limits the present invention.

Embodiment 1

The present embodiment provides a kind of methods of Ling Yaoxing and red bayberry interplanting, include the following steps：

(1) readjustment of field plot：Select the content of organic matter for 18% soil as pre- kind of ground, pre- kind of ground is dug, will Disinfectant powder is applied to pre- kind of ground bottom according to 95kg/ mus of applied amount after soil deep tillage 55cm, disinfectant powder is by following parts by weight Raw material composition：10 parts of calcium oxide, 5 parts of bur beggar-ticks extract, 5 parts of shrubby sophora extract, 3 parts of licorice, ginger peel extract 4 4 parts of part, 3 parts of aloe extract and pearl lotus extract；Then it irrigates to bottom infiltration is turned over, applies biogas residue after being exposed to the sun 2 days, make It is covered with soil is ploughed deeply, applies farm manure, carefully harrowed, by curing in 15 days, then addition soil was adjusted after ploughing deeply and ploughing Soil pH value is adjusted to 4.8 by agent, obtains plantation plot；

(2) cultivation of pekoe tea seedling：1. explant pre-processes：Clip passes through girdling root newly long pekoe tea tender shoots branch For explant, explant is cut into the branch of 5cm, 12s is impregnated with 75% alcohol, then impregnates 30min, soak period with garlic juice Between be constantly stirred with glass bar, after the completion of immersion with sterile water by explant rinse 5 times, then explant is placed on ultra-clean Its surface moisture natural air drying is waited on workbench, cuts off the blade of explant and old notch, is then cut into 2 armpits of 1.5cm bands Leaf stem section, then 8min is handled with 2% sodium hypochlorite, aseptic water washing 3 times blots stem segment with axillary bud with filter paper, obtains tissue training Foster explant is spare；2. the cultivation of culture medium：A. Fiber differentiation：The explant of acquisition is first inoculated on inducing culture, Culture 35 days is 1 by dark and light application time ratio:8 carry out Fiber differentiation, and evoked callus is differentiated to form adventitious bud；It is described Inducing culture contains MS culture medium+1.5mg/L KT+0.5mg/L 6-BA+0.1mg/L IBA+200mg/L Ca (No₃)；b. Shoot proliferation culture：Adventitious bud is transferred on proliferated culture medium and is cultivated 35-40 days, Regenerated plant is obtained；The proliferated culture medium Ingredient be：MS+1.0mg/L KT+0.2mg/L 6-BA+0.1mg/L IBA+200mg/L Ca(No₃)；C. culture of rootage：It will The Regenerated plant that Multiplying culture obtains is inoculated into root media and carries out culture of rootage, and culture obtains rooted seedling after 15 days, then will Rooted seedling goes to seeding room and carries out hardening；The ingredient of the root media is：MS+1.0mg/L ABT+0.2mg/L IBA；③ Hardening：, by being taken out in culture medium, the culture medium of seedling root will be cleaned with tap water, is used by the pekoe tea shoot of tissue cultures The carbendazim or Bravo solution of 1000 times of dilution impregnate 10 minutes, and then plantation is to being mixed with peat soil, garden mould and perlite In compost seed plate and root water is sprayed, covers upper plastic film water conservation on plant upper layer, it is 75% to keep humidity, 23 DEG C of temperature, Culture can be obtained pekoe tea seedling after 20 days；

(3) pekoe tea is planted：The organic fertilizer of 1200kg is applied per acre, and organic fertilizer is made of the raw material of following parts by weight：Straw 20 parts of stalk powder, 18 parts of vinasse, 15 parts of mushroom dreg, 3 parts of pork liver powder, 5 parts of malt, 6 parts of coconut shell flour, 5 parts of weathered coal and EM bacterium 1 Part；Kind of a planting ditch is opened up according to the seeding row spacing of 10cm × 150cm in plantation field, the depth × width for planting planting ditch is 40cm × 60cm, will be white Milli tea seedling, which is put into kind of planting ditch, to be colonized, and Nutrition Soil is used in combination to fill, and is filled to the thickness of Nutrition Soil and is relaxed to carry out carrying seedling when 20cm Root then proceedes to be filled with Nutrition Soil, until filling up kind of a plant hole, root water is irrigated after the completion of plantation；The Nutrition Soil by Rape cake, decomposed dung polluted water and rice bran are 3 according to mass ratio:1:After 1 mixing, it is put into frying in marmite and is made for 5 minutes；

(4) red bayberry seedling pre-processes：It selects height in 65cm, growth period and the pekoe tea same period or is longer than the high-quality poplar of pekoe tea Mei Miao carries out red bayberry seedling to be stained with root processing, specially：Red bayberry seedling root is soaked in 13% brine, immersion takes after five minutes Go out, impregnated 20 minutes in Fungicidal nutritive liquid, yellow mud is then stained in its root, you can；Wherein, Fungicidal nutritive liquid by with The raw material of lower parts by weight forms：1 part of sodium hypochlorite, 2 parts of garlic oil, 2 parts of sodium chloride, 2 parts of forchlorfenuron, 2 parts of methyl α-naphthyl acetate, unipods 1 part of golden lactone, 1 part of compound sodium nitrophenolate, 4 parts of brown sugar and appropriate amount of water；

(5) red bayberry seedling is interplanted：25 plants of tea place interplanting red bayberry seedling per acre, is interplanted in pekoe tea row, apart from pekoe tea tree 60cm or more, 10h first carries out watering wetting to planting pit before planting, and root water is irrigated after the completion of plantation；

(6) water and fertilizer management：Pouring fertilising irregularly is carried out to pekoe tea and red bayberry after interplanting, using with sterilizing function Liquid Fertilizer pour applying, to keep the relative humidity in plot for 25%；Wherein, Liquid Fertilizer is made of the raw material of following parts by weight： 10 parts of biogas slurry, 3 parts of chitosan, 4 parts of garlic oil, 4 parts of Folium Isatidis extract, 3 parts of fulvic acid, 3 parts of heteroauxin potassium, 8 parts of urea, 320 parts of 5 parts of mulberry leaf ferment, 2 parts of fermenting agent and water；It is 3 that fermenting agent, which is by mass ratio,:2:1:3 saccharomycete, yellow cud Coccus, aspergillus niger, filamentous fungus T. reesei mixing composition；

(7) pekoe tea harvests pre-treatment：It is harvested in the previous moon in pekoe tea, the girdling on each major branch of pekoe tea tree Go out width to be 0.5cm and cut off the girdling mouth of major branch bast, select to smear the girdling mouth without rainy day gas Selenium-rich nutrient solution, It was smeared every 3 days once, smears 3 times altogether wherein, Selenium-rich nutrient solution is made of the raw material of following parts by weight：1 part of Organic Selenium, carboxylic first 1000 parts of 10 parts of base sodium cellulosate, 2 parts of humic acid, 0.5 part of Tea Saponin, 1 part of potassium nitrate, 1 part of calcium chloride and distilled water；Selenium-rich is sought The applying amount of nutrient solution is 80g/ plants；What Organic Selenium obtained by the following method：By weight by 12 parts of sodium selenites and 200 parts of matter It is stirred under the speed that the ascorbic acid solution low whipping speed for measuring a concentration of 10% is 100r/min, 15 parts of bees is then added Glue continues to be stirred to react 20min to get the Organic Selenium.

Embodiment 2

(1) readjustment of field plot：Select the content of organic matter for 26% soil as pre- kind of ground, pre- kind of ground is dug, will Disinfectant powder is applied to pre- kind of ground bottom according to 115kg/ mus of applied amount after soil deep tillage 60cm, disinfectant powder is by following parts by weight Raw material composition：15 parts of calcium oxide, 7 parts of bur beggar-ticks extract, 9 parts of shrubby sophora extract, 6 parts of licorice, ginger peel extract 7 7 parts of part, 6 parts of aloe extract and pearl lotus extract；Then it irrigates to bottom infiltration is turned over, applies biogas residue after being exposed to the sun 2 days, make It is covered with soil is ploughed deeply, applies farm manure, carefully harrowed, by curing in 17 days, then addition soil was adjusted after ploughing deeply and ploughing Soil pH value is adjusted to 5.4 by agent, obtains plantation plot；

(2) cultivation of pekoe tea seedling：1. explant pre-processes：Clip passes through girdling root newly long pekoe tea tender shoots branch For explant, explant is cut into the branch of 6cm, 14s is impregnated with 75% alcohol, then impregnates 40min, soak period with garlic juice Between be constantly stirred with glass bar, after the completion of immersion with sterile water by explant rinse 6 times, then explant is placed on ultra-clean Its surface moisture natural air drying is waited on workbench, cuts off the blade of explant and old notch, is then cut into 2 armpits of 1.7cm bands Leaf stem section, then 9min is handled with 2% sodium hypochlorite, aseptic water washing 4 times blots stem segment with axillary bud with filter paper, obtains tissue training Foster explant is spare；2. the cultivation of culture medium：A. Fiber differentiation：The explant of acquisition is first inoculated on inducing culture, Culture 37 days is 1 by dark and light application time ratio:8 carry out Fiber differentiation, and evoked callus is differentiated to form adventitious bud；It is described Inducing culture contains MS culture medium+1.5mg/L KT+0.5mg/L 6-BA+0.1mg/L IBA+200mg/L Ca (No₃)；b. Shoot proliferation culture：Adventitious bud is transferred on proliferated culture medium and is cultivated 37 days, Regenerated plant is obtained；The proliferated culture medium at It is divided into：MS+1.0mg/L KT+0.2mg/L 6-BA+0.1mg/L IBA+200mg/L Ca(No₃)；C. culture of rootage：It will proliferation It cultivates obtained Regenerated plant and is inoculated into root media progress culture of rootage, culture obtains rooted seedling after 17 days, then will take root Seedling goes to seeding room and carries out hardening；The ingredient of the root media is：MS+1.0mg/L ABT+0.2mg/L IBA；3. refining Seedling：, by being taken out in culture medium, the culture medium of seedling root will be cleaned with tap water, use is dilute by the pekoe tea shoot of tissue cultures It releases 1000 times of carbendazim or Bravo solution impregnates 15 minutes, then plantation to the training for being mixed with peat soil, garden mould and perlite Root water is supported in soil seed plate and sprayed, covers upper plastic film water conservation on plant upper layer, it is 82% to keep humidity, 25 DEG C of temperature, training Pekoe tea seedling is can be obtained after supporting 25 days；

(3) pekoe tea is planted：The organic fertilizer of 1600kg is applied per acre, and organic fertilizer is made of the raw material of following parts by weight：Straw 25 parts of stalk powder, 20 parts of vinasse, 17 parts of mushroom dreg, 6 parts of pork liver powder, 7 parts of malt, 9 parts of coconut shell flour, 7 parts of weathered coal and EM bacterium 3 Part；Kind of a planting ditch is opened up according to the seeding row spacing of 12cm × 165cm in plantation field, the depth × width for planting planting ditch is 42cm × 62cm, will be white Milli tea seedling, which is put into kind of planting ditch, to be colonized, and Nutrition Soil is used in combination to fill, and is filled to the thickness of Nutrition Soil and is relaxed to carry out carrying seedling when 25cm Root then proceedes to be filled with Nutrition Soil, until filling up kind of a plant hole, root water is irrigated after the completion of plantation；The Nutrition Soil by Rape cake, decomposed dung polluted water and rice bran are 4 according to mass ratio:1:After 2 mixing, it is put into frying in marmite and is made for 7 minutes；

(4) red bayberry seedling pre-processes：It selects height in 72cm, growth period and the pekoe tea same period or is longer than the high-quality poplar of pekoe tea Mei Miao carries out red bayberry seedling to be stained with root processing, specially：Red bayberry seedling root is soaked in 15% brine, is taken after impregnating 7 minutes Go out, impregnated 28 minutes in Fungicidal nutritive liquid, yellow mud is then stained in its root, you can；Wherein, Fungicidal nutritive liquid by with The raw material of lower parts by weight forms：2 parts of sodium hypochlorite, 3 parts of garlic oil, 3 parts of sodium chloride, 3 parts of forchlorfenuron, 3 parts of methyl α-naphthyl acetate, unipods 2 parts of golden lactone, 1.5 parts of compound sodium nitrophenolate, 5 parts of brown sugar and appropriate amount of water；

(5) red bayberry seedling is interplanted：27 plants of tea place interplanting red bayberry seedling per acre, is interplanted in pekoe tea row, apart from pekoe tea tree 60cm or more, 11h first carries out watering wetting to planting pit before planting, and root water is irrigated after the completion of plantation；

(6) water and fertilizer management：Pouring fertilising irregularly is carried out to pekoe tea and red bayberry after interplanting, using with sterilizing function Liquid Fertilizer pour applying, to keep the relative humidity in plot for 27%；Wherein, Liquid Fertilizer is made of the raw material of following parts by weight： 15 parts of biogas slurry, 6 parts of chitosan, 7 parts of garlic oil, 7 parts of Folium Isatidis extract, 6 parts of fulvic acid, 6 parts of heteroauxin potassium, urea 12 Part, 7 parts of mulberry leaf ferment, 3 parts of fermenting agent and 380 parts of water；It is 4 that fermenting agent, which is by mass ratio,:2:1:3 saccharomycete, yellow Ruminococcus, aspergillus niger, filamentous fungus T. reesei mixing composition；

(7) pekoe tea harvests pre-treatment：It is harvested in the previous moon in pekoe tea, the girdling on each major branch of pekoe tea tree Go out width to be 1.5cm and cut off the girdling mouth of major branch bast, select to smear the girdling mouth without rainy day gas Selenium-rich nutrient solution, It was smeared every 4 days once, smears 4 times altogether wherein, Selenium-rich nutrient solution is made of the raw material of following parts by weight：1.5 parts of Organic Selenium, carboxylic 1100 parts of 12 parts of sodium carboxymethylcellulose pyce, 3 parts of humic acid, 0.6 part of Tea Saponin, 1.5 parts of potassium nitrate, 2 parts of calcium chloride and distilled water；It is rich The applying amount of selenium nutrient solution is 100g/ plants；What Organic Selenium obtained by the following method：By weight by 17 parts of sodium selenites with It is stirred under the speed that the ascorbic acid solution low whipping speed that 220 parts of mass concentrations are 12% is 150r/min, then adds Enter 22 parts of propolis, continues to be stirred to react 25min to get the Organic Selenium.

Embodiment 3

(1) readjustment of field plot：Select the content of organic matter for 30% soil as pre- kind of ground, pre- kind of ground is dug, will Disinfectant powder is applied to pre- kind of ground bottom according to 135kg/ mus of applied amount after soil deep tillage 65cm, disinfectant powder is by following parts by weight Raw material composition：20 parts of calcium oxide, 10 parts of bur beggar-ticks extract, 12 parts of shrubby sophora extract, 8 parts of licorice, ginger peel extraction 8 parts of 9 parts of object, 8 parts of aloe extract and pearl lotus extract；Then it irrigates to bottom infiltration is turned over, natural pond is applied after being exposed to the sun 3 days Slag is covered using soil is ploughed deeply, and is applied farm manure, is carefully harrowed, and by curing in 20 days, then adds soil after ploughing deeply and ploughing Soil pH value is adjusted to 6.0 by conditioning agent, obtains plantation plot；

(2) cultivation of pekoe tea seedling：1. explant pre-processes：Clip passes through girdling root newly long pekoe tea tender shoots branch For explant, explant is cut into the branch of 7cm, 16s is impregnated with 75% alcohol, then impregnates 50min, soak period with garlic juice Between be constantly stirred with glass bar, after the completion of immersion with sterile water by explant rinse 7 times, then explant is placed on ultra-clean Its surface moisture natural air drying is waited on workbench, cuts off the blade of explant and old notch, is then cut into 2 axillary buds of 2cm bands Stem section, then 10min is handled with 2% sodium hypochlorite, aseptic water washing 5 times blots stem segment with axillary bud with filter paper, obtains tissue cultures Explant it is spare；2. the cultivation of culture medium：A. Fiber differentiation：The explant of acquisition is first inoculated on inducing culture, is trained It supports 40 days, is 1 by dark and light application time ratio:8 carry out Fiber differentiation, and evoked callus is differentiated to form adventitious bud；It is described to lure It leads culture medium and contains MS culture medium+1.5mg/L KT+0.5mg/L 6-BA+0.1mg/L IBA+200mg/L Ca (No₃)；B. after For Multiplying culture：Adventitious bud is transferred on proliferated culture medium and is cultivated 40 days, Regenerated plant is obtained；The ingredient of the proliferated culture medium For：MS+1.0mg/L KT+0.2mg/L 6-BA+0.1mg/L IBA+200mg/L Ca(No₃)；C. culture of rootage：Proliferation is trained It supports obtained Regenerated plant and is inoculated into root media progress culture of rootage, culture obtains rooted seedling after 20 days, then by rooted seedling It goes to seeding room and carries out hardening；The ingredient of the root media is：MS+1.0mg/L ABT+0.2mg/L IBA；3. hardening： , by being taken out in culture medium, the culture medium of seedling root will be cleaned with tap water, with dilution by the pekoe tea shoot of tissue cultures 1000 times of carbendazim or Bravo solution impregnates 20 minutes, then plantation to the culture for being mixed with peat soil, garden mould and perlite In native seed plate and root water is sprayed, covers upper plastic film water conservation on plant upper layer, it is 90% to keep humidity, 26 DEG C of temperature, culture It can be obtained pekoe tea seedling after 30 days；

(3) pekoe tea is planted：The organic fertilizer of 1800kg is applied per acre, and organic fertilizer is made of the raw material of following parts by weight：Straw 30 parts of stalk powder, 22 parts of vinasse, 20 parts of mushroom dreg, 8 parts of pork liver powder, 10 parts of malt, 12 parts of coconut shell flour, 10 parts of weathered coal and EM bacterium 5 parts；Kind of a planting ditch is opened up according to the seeding row spacing of 15cm × 180cm in plantation field, the depth × width for planting planting ditch is 45cm × 65cm, will be white Milli tea seedling, which is put into kind of planting ditch, to be colonized, and Nutrition Soil is used in combination to fill, and is filled to the thickness of Nutrition Soil and is relaxed to carry out carrying seedling when 30cm Root then proceedes to be filled with Nutrition Soil, until filling up kind of a plant hole, root water is irrigated after the completion of plantation；The Nutrition Soil by Rape cake, decomposed dung polluted water and rice bran are 5 according to mass ratio:2:After 3 mixing, it is put into frying in marmite and is made for 10 minutes；

(4) red bayberry seedling pre-processes：It selects height in 90cm, growth period and the pekoe tea same period or is longer than the high-quality poplar of pekoe tea Mei Miao carries out red bayberry seedling to be stained with root processing, specially：Red bayberry seedling root is soaked in 18% brine, is taken after impregnating 9 minutes Go out, impregnated 35 minutes in Fungicidal nutritive liquid, yellow mud is then stained in its root, you can；Wherein, Fungicidal nutritive liquid by with The raw material of lower parts by weight forms：3 parts of sodium hypochlorite, 4 parts of garlic oil, 4 parts of sodium chloride, 4 parts of forchlorfenuron, 4 parts of methyl α-naphthyl acetate, unipods 3 parts of golden lactone, 2 parts of compound sodium nitrophenolate, 6 parts of brown sugar and appropriate amount of water；

(5) red bayberry seedling is interplanted：25-30 plants of red bayberry seedling is interplanted in tea place per acre, is interplanted in pekoe tea row, apart from pekoe tea tree 60cm or more, 12h first carries out watering wetting to planting pit before planting, and root water is irrigated after the completion of plantation；

(6) water and fertilizer management：Pouring fertilising irregularly is carried out to pekoe tea and red bayberry after interplanting, using with sterilizing function Liquid Fertilizer pour applying, to keep the relative humidity in plot for 30%；Wherein, Liquid Fertilizer is made of the raw material of following parts by weight： 20 parts of biogas slurry, 8 parts of chitosan, 9 parts of garlic oil, 9 parts of Folium Isatidis extract, 8 parts of fulvic acid, 8 parts of heteroauxin potassium, urea 16 Part, 10 parts of mulberry leaf ferment, 4 parts of fermenting agent and 450 parts of water；It is 5 that fermenting agent, which is by mass ratio,:3:1:3 saccharomycete, yellow Ruminococcus, aspergillus niger, filamentous fungus T. reesei mixing composition；

(7) pekoe tea harvests pre-treatment：It is harvested in the previous moon in pekoe tea, the girdling on each major branch of pekoe tea tree Go out width to be 2.5cm and cut off the girdling mouth of major branch bast, select to smear the girdling mouth without rainy day gas Selenium-rich nutrient solution, It was smeared every 5 days once, smears 5 times altogether wherein, Selenium-rich nutrient solution is made of the raw material of following parts by weight：2 parts of Organic Selenium, carboxylic first 1200 parts of 15 parts of base sodium cellulosate, 4 parts of humic acid, 0.8 part of Tea Saponin, 2 parts of potassium nitrate, 3 parts of calcium chloride and distilled water；Selenium-rich is sought The applying amount of nutrient solution is 120g/ plants；What Organic Selenium obtained by the following method：By weight by 22 parts of sodium selenites and 250 parts It is stirred under the speed that the ascorbic acid solution low whipping speed that mass concentration is 15% is 200r/min, is then added 30 parts Propolis continues to be stirred to react 30min to get the Organic Selenium.

Comparative example 1

This comparative example is identical with the implantation methods of embodiment 2, but is pre-processed to plot without using disinfectant powder, Other methods are identical with embodiment 2.

Comparative example 2

This comparative example is identical with the implantation methods of embodiment 2, but in carrying out preprocessing process to red bayberry seedling, The immersion treatment of Fungicidal nutritive liquid is not carried out to seedling, other methods are identical with embodiment 2.

Comparative example 3

This comparative example is identical with the implantation methods of embodiment 2, but before planting pekoe tea, does not apply organic Fertilizer, other methods are identical with embodiment 2.

Comparative example 4

This comparative example is identical with the implantation methods of embodiment 2, but the Liquid Fertilizer with sterilizing function applied changes For commercially available common fertilizer, other methods are identical with embodiment 2.

Comparative example 5

This comparative example is identical with the implantation methods of embodiment 2, but only plants Ling Yaoxing, other methods and reality It is identical to apply example 2.

Comparative example 6

This comparative example is identical with the implantation methods of embodiment 2, but only plants red bayberry, other methods and embodiment 2 It is identical.

Comparative example 7

This comparative example is identical with the implantation methods of embodiment 2, but removes step (2), directly chooses finished product pekoe Tea shoot is planted, and other methods are identical with embodiment 2.

Comparative example 8

This comparative example is identical with the implantation methods of embodiment 2, but removes step (7) and pick pre-treatment to pekoe tea The step of, other methods are identical with embodiment 2.

Compliance test result

The practical value of implantation methods in order to further illustrate the present invention does following testing experiment several times：

Testing experiment 1

Experiment is completed in the Experimental Base of this research institute, is planted according to the method for embodiment 1-3 and comparative example 1-8, After red bayberry seedling plants 30d, each processing randomly selects 3 plants of red bayberry seedling and 3 plants of pekoe tea seedling, is surveyed respectively with ruler The seedling height of seedling of fixed 3 plants of red bayberry seedlings and 3 plants of pekoe tea shoots, then initial height of seedling is subtracted, growing height is obtained, and be averaged.Tool Body situation is shown in Table 1：

Table 1

As seen from the above table：The seedling of embodiment 1-3 is apparently higher than comparative example 1-8, illustrates the disinfectant powder, organic of the application Place before fertilizer, nutrient solution, Liquid Fertilizer, Ling Yaoxing and the method for red bayberry interplanting, pekoe tea method for cultivating seeds, pekoe tea picking The cultivation etc. of reason, pekoe tea seedling can effectively improve Ling Yaoxing and the growth ability of red bayberry.

Testing experiment 2

Single factor experiment is carried out to disinfectant powder, plantation plot is equally divided into following groups, in the way of table 2 Reason：

Table 2

Group	Experiment content
		Test group 1	Plot is handled without using disinfectant powder, other processing modes are same as Example 1
Test group 2	Calcium oxide is not contained in the disinfectant powder used, other processing modes are same as Example 1
		Test group 3	Bur beggar-ticks extract is not contained in the disinfectant powder used, other processing modes are same as Example 1
Test group 4	Shrubby sophora extract is not contained in the disinfectant powder used, other processing modes are same as Example 1
		Test group 5	Licorice is not contained in the disinfectant powder used, other processing modes are same as Example 1
Test group 6	Ginger peel extract is not contained in the disinfectant powder used, other processing modes are same as Example 1
		Test group 7	Aloe extract is not contained in the disinfectant powder used, other processing modes are same as Example 1
Test group 8	Pearl lotus extract is not contained in the disinfectant powder used, other processing modes are same as Example 1
		Control group	Plot is handled according to the method for embodiment 1

After plot is carried out above-mentioned processing, from 30d after respective sowing day, surviving for red bayberry seedling and pekoe tea shoot is counted Whether rate, germination percentage, blade integrity degree and measurement soil are containing the harmful components for being unfavorable for crop growth.Concrete condition is shown in Table 3

Table 3

Group	Survival rate (%)	Germination percentage (%)	Blade integrity degree (%)	Harmful components in soil
					Test group 1	69.92	57.58	81.24	Largely
Test group 2	83.17	73.12	90.89	On a small quantity
					Test group 3	79.63	70.93	91.02	On a small quantity
Test group 4	81.62	68.92	90.32	On a small quantity
					Test group 5	82.23	72.26	92.66	On a small quantity
Test group 6	81.98	71.62	90.91	On a small quantity
					Test group 7	84.42	73.12	92.88	On a small quantity
Test group 8	74.13	62.35	87.25	On a small quantity
					Control group	99.25	83.21	99.61	Nothing

As seen from the above table, the survival rate of test group 1-8, germination percentage, blade integrity degree are all significantly lower than control group, and test Group 1-8 has found harmful components in the soil, illustrates that disinfectant powder used in this application can carry out soil adequately disinfected sterilizing, It still plays a role after planting a period of time, hence it is evident that improve survival rate, the germination percentage of seedling, and ensured the blade of seedling Integrity degree；Test group 3, test group 7 blade integrity degree be significantly lower than control group and other groups, illustrate the disinfection of the application Powder can play the role of sterilizing, antivirus and expelling parasite, and calcium oxide, orange peel extract, forsythia suspense extraction, ginger peel carry in disinfectant powder It takes object, eucalyptus extracts, sodium chloride and pearl lotus extract indispensable, the blade integrity degree of seedling can be significantly improved.

Testing experiment 3

Step (4) does following to having a competition the practical value of the cultivation of pekoe tea seedling in order to further illustrate the present invention It tests：Implementation people is divided into following four groups and does contrast test：

First group：Method for tissue culture described in embodiment 1；

Second group：Method for tissue culture described in embodiment 2；

Third group：Method for tissue culture described in embodiment 3；

4th group：Culture medium prescription is changed to MS culture medium+2.5mg/L 6-BA+0.5mg/L IBA-D+30g/L sucrose, Other modes are carried out in strict accordance with embodiment 2.

Above-mentioned four groups are observed in tissue-cultured seedling situation, the rooted seedling number, rooting rate and Duo Gen rates of each group is recorded, the results are shown in Table 4；

4 four groups of pekoe tea tissue-cultured seedling situation tables of table

Group	Explant number	Rooted seedling number (item)	Rooting rate (%)	More rates (%)
					First group	23	12	92	34
Second group	24	14	95	36
					Third group	22	13	93	35
4th group	23	5	76	12

According to upper table：The pekoe tea seedling that method for tissue culture using the present invention is cultivated, rooted seedling number is more, Rooting rate and more rate height are suitable for large-scale plantation, are worthy to be popularized.

Testing experiment 4

Step (7) harvests pekoe tea the practical value of pre-treatment in order to further illustrate the present invention, does following to having a competition It tests：It is divided into following three groups, every group ten plants：First group：Using the identical sodium selenite solution of selenium content replace Selenium-rich nutrient solution into Row girdling mouth is smeared；Second group：Processing method in step (7) described in embodiment 2；Third group：Blank control group, before harvesting Pekoe tea is not handled, the total Se content of every group of random sampling detection pekoe tea cured leaf and organic selenium content the results are shown in Table 5.

Total Se content, the organic selenium content of pekoe tea cured leaf in 5 each experimental group of table

	Total Se content (μ g/kg)	Organic Selenium accounting (%)
			First group	814	87.5
Second group	892	89.4
			Third group	735	65.4

From the results shown in Table 5, the total Se content of the solution of the present invention and organic selenium content are preferable.

In conclusion during the interplanting of the present invention, a series of used processing methods：The cultivation of pekoe tea seedling, Disinfectant powder, organic fertilizer, nutrient solution and Liquid Fertilizer etc. can be effectively improved the growing way of seedling, and the solution of the present invention is an entirety, It is indispensable.

Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.

Claims

1. a kind of method of Ling Yaoxing and red bayberry interplanting, which is characterized in that include the following steps：

(1) readjustment of field plot：It is the soil of 18-30% as pre- kind of ground to select the content of organic matter, is dug to pre- kind of ground, will be native Earth is ploughed deeply after 55-65cm and applies disinfectant powder to pre- kind of ground bottom according to 95-135kg/ mu of applied amount, and irrigation is to turning over bottom Infiltration, applies biogas residue after being exposed to the sun 2-3 days, is covered using soil is ploughed deeply, and application farm manure is carefully harrowed, ripe by 15-20 days Change, then soil pH value is adjusted to 4.8-6.0 by addition soil conditioner after ploughing deeply and ploughing, and obtains plantation plot；

(2) cultivation of pekoe tea seedling：1. explant pre-processes：By girdling root, newly long pekoe tea tender shoots branch is outer to clip Explant is cut into the branch of 5-7cm by implant, impregnates 12-16s with 75% alcohol, then impregnates 30-50min, leaching with garlic juice It is constantly stirred with glass bar during bubble, explant is rinsed 5-7 times with sterile water after the completion of immersion, then puts explant Its surface moisture natural air drying is waited on superclean bench, is cut off the blade of explant and old notch, is then cut into 1.5-2cm 2 axillary bud stem sections of band, then 8-10min is handled with 2% sodium hypochlorite, aseptic water washing 3-5 times blots band axillary bud stem with filter paper Section, the explant for obtaining tissue cultures are spare；2. the cultivation of culture medium：A. Fiber differentiation：The explant of acquisition is first inoculated into It on inducing culture, cultivates 35-40 days, is 1 by dark and light application time ratio:8 carry out Fiber differentiation, evoked callus differentiation Form adventitious bud；The inducing culture contains MS culture medium+1.5mg/L KT+0.5mg/L 6-BA+0.1mg/L IBA+ 200mg/L Ca(No₃)；B. shoot proliferation culture：Adventitious bud is transferred on proliferated culture medium and is cultivated 35-40 days, subculture is obtained Seedling；The ingredient of the proliferated culture medium is：MS+1.0mg/L KT+0.2mg/L 6-BA+0.1mg/L IBA+200mg/L Ca (No₃)；C. culture of rootage：The Regenerated plant that Multiplying culture obtains is inoculated into root media and carries out culture of rootage, cultivates 15-20 Rooted seedling is obtained after it, rooted seedling, which is then gone to seeding room, carries out hardening；The ingredient of the root media is：MS+ 1.0mg/L ABT+0.2mg/L IBA；3. hardening：By the pekoe tea shoot of process tissue cultures by being taken out in culture medium, with originally Water cleans the culture medium of seedling root, is impregnated 10-20 minutes with the carbendazim or Bravo solution that dilute 1000 times, is then planted It plants and is mixed in the compost seed plate of peat soil, garden mould and perlite and sprays root water, cover upper plastic film on plant upper layer Water conservation, holding humidity are 75-90%, and 23-26 DEG C of temperature can be obtained pekoe tea seedling after cultivating 20-30 days；

(3) pekoe tea is planted：The organic fertilizer for applying 1200-1800kg per acre, in plantation field according to 10-15cm × 150-180cm Seeding row spacing open up kind of a planting ditch, plant planting ditch depth × width be 40-45cm × 60-65cm, pekoe tea seedling is put into kind of planting ditch Field planting, is used in combination Nutrition Soil to fill, and fills to the thickness of Nutrition Soil to carry out carrying Miao Shugen when 20-30cm, then proceedes to use nutrition Soil is filled, until filling up kind of a plant hole, root water is irrigated after the completion of plantation；The Nutrition Soil is by rape cake, decomposed dung polluted water With rice bran according to mass ratio be 3-5:1-2:After 1-3 mixing, it is put into frying 5-10 minutes in marmite and is made；

(4) red bayberry seedling pre-processes：It selects height in 65-90cm, growth period and the pekoe tea same period or is longer than the high-quality poplar of pekoe tea Mei Miao carries out red bayberry seedling to be stained with root processing, specially：Red bayberry seedling root is soaked in the brine of 13-18%, impregnates 5-9 points It is taken out after clock, is impregnated 20-35 minutes in Fungicidal nutritive liquid, yellow mud is then stained in its root, you can；

(5) red bayberry seedling is interplanted：25-30 plants of red bayberry seedling is interplanted in tea place per acre, is interplanted in pekoe tea row, apart from pekoe tea tree 60cm More than, 10-12h first carries out watering wetting to planting pit before planting, and root water is irrigated after the completion of plantation；

(6) water and fertilizer management：Pouring fertilising irregularly is carried out to pekoe tea and red bayberry after interplanting, using the liquid with sterilizing function Fertilizer pour applying, to keep the relative humidity in plot for 25-30%；

(7) pekoe tea harvests pre-treatment：Harvested in the previous moon in pekoe tea, on each major branch of pekoe tea tree girdling go out width Degree is 0.5-2.5cm and cuts off the girdling mouth of major branch bast, and selection smears the girdling mouth without rainy day gas Selenium-rich nutrient solution, Primary, smearing 3-5 times altogether was smeared every 3-5 days.

2. the method for a kind of Ling Yaoxing according to claim 1 and red bayberry interplanting, which is characterized in that the step (1) disinfectant powder in is made of the raw material of following parts by weight：10-20 parts of calcium oxide, 5-10 parts of bur beggar-ticks extract, kuh-seng extraction 4-8 parts of 5-12 parts of object, 3-8 parts of licorice, 4-9 parts of ginger peel extract, 3-8 parts of aloe extract and pearl lotus extract.

3. the method for a kind of Ling Yaoxing according to claim 1 and red bayberry interplanting, which is characterized in that the step (4) the Fungicidal nutritive liquid in is made of the raw material of following parts by weight：1-3 parts of sodium hypochlorite, 2-4 parts of garlic oil, sodium chloride 2-4 Part, 2-4 parts of forchlorfenuron, 2-4 parts of methyl α-naphthyl acetate, 1-3 parts of witchweed lactone, 1-2 parts of compound sodium nitrophenolate, 4-6 parts of brown sugar and water are suitable Amount.

4. the method for a kind of Ling Yaoxing according to claim 1 and red bayberry interplanting, which is characterized in that the step (3) organic fertilizer in is made of the raw material of following parts by weight：20-30 parts of straw powder, 18-22 parts of vinasse, mushroom dreg 15-20 Part, 3-8 parts of pork liver powder, 5-10 parts of malt, 6-12 parts of coconut shell flour, 5-10 parts of weathered coal and 1-5 parts of EM bacterium.

5. the method for a kind of Ling Yaoxing according to claim 1 and red bayberry interplanting, which is characterized in that the step (6) Liquid Fertilizer in is made of the raw material of following parts by weight：10-20 parts of biogas slurry, 3-8 parts of chitosan, 4-9 parts of garlic oil, smalt 4-9 parts of leaf extract, 3-8 parts of fulvic acid, 3-8 parts of heteroauxin potassium, 8-16 parts of urea, 5-10 parts of mulberry leaf ferment, fermenting agent 2-4 parts and 320-450 parts of water.

6. the method for a kind of Ling Yaoxing according to claim 5 and red bayberry interplanting, which is characterized in that the zymophyte It is 3-5 that agent, which is by mass ratio,:2-3:1:3 saccharomycete, yellow Ruminococcus, aspergillus niger, filamentous fungus T. reesei mixing group At.

7. the method for a kind of Ling Yaoxing according to claim 1 and red bayberry interplanting, which is characterized in that the step (7) Selenium-rich nutrient solution in is made of the raw material of following parts by weight：1-2 parts of Organic Selenium, 10-15 parts of sodium carboxymethylcellulose, corruption Grow 1000-1200 parts of 2-4 parts sour, 0.5-0.8 parts of Tea Saponin, 1-2 parts of potassium nitrate, 1-3 parts of calcium chloride and distilled water；Selenium-rich nutritive The applying amount of liquid is 80-120g/ plants.

8. the method for a kind of Ling Yaoxing according to claim 2 and red bayberry interplanting, which is characterized in that the disinfectant powder It is made of the raw material of following parts by weight：15 parts of calcium oxide, 7 parts of bur beggar-ticks extract, 9 parts of shrubby sophora extract, licorice 6 Part, 7 parts of ginger peel extract, 6 parts of aloe extract and 7 parts of pearl lotus extract.

9. the method for a kind of Ling Yaoxing according to claim 7 and red bayberry interplanting, which is characterized in that the Organic Selenium It obtains by the following method：It is by weight the anti-of 10-15% by 12-22 parts of sodium selenites and 200-250 parts of mass concentrations It is stirred under the speed that bad hematic acid solution low whipping speed is 100-200r/min, 15-30 parts of propolis is then added, continue to stir Reaction 20-30min is mixed to get the Organic Selenium.