CN107981065A - Composite bacteria liquid, the fermented feed using it and its application in preparation is used to improve the feed of weanling pig intestinal health - Google Patents
Composite bacteria liquid, the fermented feed using it and its application in preparation is used to improve the feed of weanling pig intestinal health Download PDFInfo
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Abstract
A kind of fermented feed the present invention provides composite bacteria liquid, using it and its application in preparation is used to improve the feed of weanling pig intestinal health, are related to the technical field of feed processing.The composite bacteria liquid, which has, be carbon source using a variety of glycogens such as carbohydrate such as glucose, xylose, sucrose, molasses, using urea and nitric acid as nitrogen source, and need not growth factor the characteristics of can growing, protein yield height.The fermented feed, containing multiple beneficial microorganism and organic acid, can improve livestock intestinal environment.The preparation method of the fermented feed, technique is simple, simple and convenient, is easy to all kinds of raisers, production Feed Enterprise operation.The complete feed, can maintain intestinal health while without antibiotic.The composite bacteria liquid is preparing the application in improving weanling pig intestinal health feed.Pigling immunity can be strengthened, reduce diarrhea of weaned piglets rate, efficiency of feed utilization is improved, reduce aquaculture cost.
Description
Technical field
The present invention relates to field of feed processing, more particularly, to a kind of composite bacteria liquid, using its fermented feed and its
Prepare the application for being used to improve in the feed of weanling pig intestinal health.
Background technology
At present in pig industry, this mode of early weaning piglet can significantly improve sow production performance, reduce and raise
Form this.However, since the digestive function development of piglet is not perfect enough, enzyme system is simultaneously unsound, autoimmunity lowly etc. because
Element, easily induces early weaning piglet syndrome, causes piglet functional disturbances of gastrointestinal tract, and growth slows down or stagnate even death,
Great economic loss is caused to pig production.The quality of grice diarrhoea control, which is increasingly becoming, weighs piglet culture success or not
One of important indicator, also becomes the problem of feedstuff industry is in the urgent need to address.
Therefore, antibiotic is added in feed becomes the main means for solving this phenomenon.It is well known that feeding antibiotic
Harmful microbe growth and breeding in piglet digestive are can inhibit, the consumption of endotrophic material is reduced, suppresses or kill certain
A little pathogenic microorganisms, strengthen the disease resistance of piglet, prevent disease.However, for serious intestines problem, antibiotic is being used
While treatment is killed or suppresses pernicious bacteria, also profitable strain in enteron aisle is also eliminated or inhibited.Some researches show that many
The main reason for vaccine and medicine do not have the death rate for reducing piglet, cause diarrhea of weaned piglets is flora imbalance and digestive ferment
Deficiency.
Enteron aisle is the organ that nutrient digestion absorption is mainly responsible in livestock and poultry body, and the development of enteron aisle and health status are to poultry
The growth of fowl and health are most important.Young livestock, such as newborn piglet, enteron aisle are among Rapid development, and structure and function are still
Imperfection, digesting and assimilating for nutrient have significant limitation with immunization barrier function.In addition, weaning stress causes intestinal mucosa to wither
Contracting, gut epithelium integrality and gut barrier are destroyed and function of intestinal canal is disorderly.Intestinal tissue reaches maturity, damages and repair
The multiple, improvement of structure and the maintenance of function need special nutrient and the substantial amounts of nutrient of consumption, therefore nutrition regulation is to ensure
Intestine of young pigs healthy development and the most effective means for exercising normal function.Some researches show that, more than 85% grice diarrhoea be by
Caused by enteric microorganism is unbalance, it is not necessary to antibiosis extract for treating, need to only correct microecological balance can rehabilitation, and how to correct micro-
The ecological balance one of is a problem to be solved.
Fermented feed has improvement feed palatability, raising feed nutritive value, production cost is relatively low and makes full use of more
The features such as kind feedstuff, research and development potentiality are very big, are the important directions of nonreactive safe feed development now.Fermented feed pH
Generally below 4.5, containing the organic acids such as substantial amounts of lactic acid, lactic acid bacteria and other beneficial microbes, and in fermented feed
Harmful microorganism can be suppressed by sour environment and other antibacterial substances.
Therefore, found in terms of fermented feed is developed improve intestine of young pigs flora ecological balance relation method, with up to
To its efficiency of feed utilization is improved, enhancing pigling immunity, prevention diarrhea, are to have the problem of to be solved.
In view of this, it is special to propose the present invention.
The content of the invention
The first object of the present invention is the provision of a kind of composite bacteria liquid, and alleviating shortage one kind in the prior art can have
Imitate the technical problem of the composite bacteria agent of albumen and macromolecular carbohydrate in fermentation substrate.
The second object of the present invention is to provide a kind of fermented feed, alleviates a kind of fermentation of shortage in the prior art and fill
Point, the technical problem rich in beneficial bacterium and the easy fermented feed for absorbing nutriment.
The third object of the present invention is to provide a kind of preparation method of fermented feed, alleviates and lack one in the prior art
Kind it is capable of the technical problem of the abundant fermented feed of fermentation substrate.
The fourth object of the present invention is the provision of a kind of complete feed for containing above-mentioned fermented feed, alleviates existing
Technology lacks a kind of feed that intestinal health is able to maintain that while be free of antibiotic.
The fifth object of the present invention is the provision of a kind of above-mentioned composite bacteria liquid and is preparing improvement weanling pig intestinal health
Application in feed, alleviates in the prior art that Intestinal Microflora of Weanling Piglets is unbalance and diarrhea lures by causing containing antibiotic feed
The technical problem of the early weaning piglet syndrome of hair.
The present invention provides a kind of composite bacteria liquid, the composite bacteria liquid mainly includes following component：Aspergillus oryzae, bifid bar
Bacterium, clostridium butyricum, Lactobacillus delbrueckii and candida utili.
Further, the composite bacteria liquid is counted in parts by weight mainly includes following component：1~5 part of aspergillus oryzae, bifid
5~15 parts of 1~5 part of bacillus, clostridium butyricum 1~5,1~5 part of Lactobacillus delbrueckii and candida utili.
Further, the composite bacteria liquid is counted in parts by weight mainly includes following component：1~3 part of aspergillus oryzae, bifid
8~12 parts of 1~3.5 part of bacillus, clostridium butyricum 1~3,1~2.5 part of Lactobacillus delbrueckii and candida utili.
Further, the composite bacteria liquid is counted in parts by weight mainly includes following component：1 part of aspergillus oryzae, Bifidobacterium
10 parts of 2 parts, 1 part of clostridium butyricum, 2 parts of Lactobacillus delbrueckii and candida utili.
The present invention provides a kind of fermented feed, the raw material of the fermented feed includes above-mentioned composite bacteria liquid.
The present invention provides a kind of preparation method of fermented feed, the preparation method mainly includes the following steps：Respectively
Matrix and the composite bacteria liquid are prepared, the composite bacteria liquid being prepared is inoculated in matrix, is mixed, fermentation, obtains the hair
Ferment feed.
Further, the matrix is counted including following component in parts by weight：50~62 parts of dregs of beans, 8~15 parts of wheat bran, sugar
2~8 parts of honey, 5~13 parts of corn flour, 5~12 parts of peanut meal, 2~10 parts of rapeseed dregs, 4~11 parts of corn protein powder, soybean point
1~8 part from albumen and 0.5~1.5 part of salt；
Preferably, 52 parts of dregs of beans, 8 parts of wheat bran, 6 parts of molasses, 8 parts of corn flour, 8 parts of peanut meal, 5 parts of rapeseed dregs, corn egg
1 part of 6 parts of white powder, 6 parts of soybean protein isolate and salt.
Further, the addition of composite bacteria liquid described in the matrix is 5~15% (v/w).
The present invention provides a kind of complete feed, includes above-mentioned fermented feed.
The present invention provides a kind of above-mentioned composite bacteria liquid to prepare the application in improving weanling pig intestinal health feed.
Composite bacteria liquid provided by the invention have can utilize a variety of glycogens such as carbohydrate such as glucose, xylose, sucrose, molasses
For carbon source, using urea and nitric acid as nitrogen source, and the characteristics of be not required growth factor to grow, protein yield is high.This hair
The fermented feed of bright offer, containing multiple beneficial microorganism and organic acid, can improve livestock intestinal environment.It is provided by the invention
The preparation method of fermented feed, technique is simple, simple and convenient, is easy to all kinds of raisers, production Feed Enterprise operation.The present invention carries
The complete feed of confession, can maintain intestinal health while without antibiotic.The composite bacteria liquid provided by the invention is being made
The standby application improved in weanling pig intestinal health feed.Pigling immunity can be strengthened, reduce diarrhea of weaned piglets rate, improved
Efficiency of feed utilization, reduces aquaculture cost.
Embodiment
Technical scheme is clearly and completely described below in conjunction with embodiment, it is clear that described reality
It is part of the embodiment of the present invention to apply example, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this area
Art personnel all other embodiments obtained without making creative work, belong to the model that the present invention protects
Enclose.
The present invention provides a kind of composite bacteria liquid, above-mentioned composite bacteria liquid mainly includes following component：Aspergillus oryzae, globefish bifid bar
Bacterium, clostridium butyricum, Lactobacillus delbrueckii and candida utili.
Composite bacteria liquid provided by the invention includes aspergillus oryzae Aspergillus oryzae, Bifidobacterium
Bifidobacterium, clostridium butyricum Clostridium butyricum and German-style lactobacillus Lactobacillus
Delbrueckii, the composite bacteria liquid can produce a variety of enzymes, such as amylase, pepsin etc. in fermentation, can be by feed
Starch, the larger molecular organics matter such as protein is degraded to monose, Shuan Tang, various amino acid and other nutrients of small molecule
Matter, makes livestock be easier to absorb.Aspergillus oryzae has abundant albumen enzyme system, can produce acid, neutral and basic protein, stability
Height, can eliminate anti-nutritional factors and antigen protein in fermentation substrate；Bifidobacterium is considered as that the mankind and other homoiothermies move
Key microorganisms in thing enteron aisle, and a kind of well-known beneficial bacteria of intestinal tract, can safeguard the balance of normal intestinal flora,
Suppress the growth of pathogenic microorganism；Clostridium butyricum is also known as clostridium butyricum, and having improves ecological environment in enteron aisle, reduces enteron aisle disease
Disease, improves the function of immunity, there is stronger antagonism to pathogenic bacteria, can suppress escherichia coli, typhoid bacillus, dysentery
The growth and breeding of bacillus.Lactobacillus delbrueckii category homofermentative lactic bacteria, its own contains and is metabolized a variety of nutriments of production, disappears
Change enzyme and antibacterial substance, play an important role to promoting animal body Nutrients Digestion to absorb and adjusting animal physiological function, in addition, moral
Family name's lactobacillus has good intestinal colonisation ability, adjusts intestinal flora balance, safeguards intestinal health.
In an optional embodiment, above-mentioned composite bacteria liquid is counted in parts by weight mainly includes following component：Meter Qu
5~15 parts of mould 1~5 part, 1~5 part of Bifidobacterium, clostridium butyricum 1~5,1~5 part of Lactobacillus delbrueckii and candida utili.
Wherein, aspergillus oryzae for example can be but be not limited to 1 part, 2 parts, 3 parts, 4 parts or 5 parts；1~5 part of Bifidobacterium, example
It such as can be but be not limited to 1 part, 2 parts, 3 parts, 4 parts or 5 parts；1~5 part of clostridium butyricum, for example, can be but be not limited to 1 part, 2
Part, 3 parts, 4 parts or 5 parts；1~5 part of Lactobacillus delbrueckii, such as can be but be not limited to 1 part, 2 parts, 3 parts, 4 parts or 5 parts；
5~15 parts of candida utili bacterium, for example, can be but be not limited to 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts,
13 parts, 14 or 15 parts.
In one preferred embodiment, above-mentioned composite bacteria liquid is counted in parts by weight mainly includes following component：Meter Qu
10 parts of mould 1 part, 2 parts of Bifidobacterium, 1 part of clostridium butyricum, 2 parts of Lactobacillus delbrueckii and candida utili.
In an optional embodiment, above-mentioned composite bacteria liquid is by aspergillus oryzae seed liquor, globefish Bifidobacterium seed liquid, fourth
Sour clostridium seed liquor, Lactobacillus delbrueckii seed liquor and candida utili bacterium seed liquor are mixed.
In one preferred embodiment, the preparation method of above-mentioned aspergillus oryzae seed liquor is as follows：
The aspergillus oryzae strain activated is taken, the culture medium equipped with SP is seeded to, 20h, shaking table is cultivated in 37 DEG C of constant-temperature tables
Rotating speed is 200rpm, is spare seed liquor.
SP culture medium prescriptions are as follows：Soluble starch 20g/L, potassium dihydrogen phosphate 1g/L, magnesium sulfate 0.5g/L, ammonium sulfate
0.5g/L, agar 20g/L, is dissolved in soya-bean cake diffusion juice.
Soya-bean cake diffusion juice preparation method is as follows：100 grams of soya-bean cakes, add water 500ml, when immersion 4 is small, boil 3-4 it is small when, gauze
Natural filtration, takes liquid, adjusts to 5 Baume degrees.
In one preferred embodiment, the preparation method of above-mentioned Bifidobacterium seed liquid is as follows：
It is to be trained in 7.0,30 DEG C of constant-temperature tables to take the Bifidobacterium activated to be seeded to the culture medium equipped with TPY to adjust pH value
24h, shaking speed 180rpm are supported, is spare seed liquor.
TPY culture medium prescriptions are as follows：Peptone 15g/L, dusty yeast 2g/L, glucose 20g/L, soluble starch 0.5g/
L, sodium chloride 5g/L, 5% cysteine 10ml, tomato leachate 400Ml, Tween 80 1ml, liver extracting solution 80Ml, agar
20g/L, be dissolved in distilled water.
In one preferred embodiment, above-mentioned Bifidobacterium is globefish Bifidobacterium.Globefish Bifidobacterium is a kind of separation
Probiotics in chitling road, it is played a crucial role for adjusting intestinal flora balance, and can effectively alleviate intestines
Road inflammation and symptom of irritable bowel syndrome, prevent antibiotic-associated diarrhea.
In one preferred embodiment, the preparation method of above-mentioned butyrate spindle bacillus seed liquid is as follows：
The clostridium butyricum activated is taken to be seeded in the RCM culture mediums that 200mL is preset, it is 7.3,37 DEG C to adjust pH values
18h, shaking speed 220rpm are cultivated in constant-temperature table, is spare seed liquor.
RCM culture medium prescriptions are as follows：Yeast extract 3g/L, beef extract 10g/L, trypsase peptone 10g/L, glucose
5g/L, soluble starch 1g/L, sodium chloride 5g/L, sodium acetate trihydrate 3g/L, cysteine hydrochloride 0.15g/L, be dissolved in steaming
Distilled water.
In one preferred embodiment, the preparation method of above-mentioned Lactobacillus delbrueckii seed liquor is as follows：
The Lactobacillus delbrueckii activated is taken to be seeded to the culture medium equipped with MSR, adjusting pH value is 6.2,37 DEG C of constant-temperature tables
Middle culture 24h, shaking speed 220rpm, are spare seed liquor.
The culture medium prescription of MSR is as follows：Glucose 20g/L, peptone 10g/L, 10 g/L of beef extract, yeast extract
5g/L, dipotassium hydrogen phosphate 2g/L, anhydrous sodium acetate 5g/L, Triammonium citrate 2g/L, bitter salt 0.58g/L, four hydrations
Manganese sulfate 0.25g/L；Tween 80 1mL, for distilled water.
In one preferred embodiment, the preparation method of above-mentioned candida utili bacterium seed liquor is as follows：
The candida utili bacterium activated is taken to be seeded in the culture medium equipped with pre-optimized mistake, it is 5.5 to adjust pH value,
24h, shaking speed 122rpm are cultivated in 30 DEG C of constant-temperature tables, is spare seed liquor.
Culture medium prescription is：Peptone 30g/L, sucrose 60g/L, potassium dihydrogen phosphate 2g/L, magnesium sulfate 1.5g/L, ammonium sulfate
1g/L, is dissolved in distilled water.
The present invention provides a kind of fermented feed, the raw material of the fermented feed includes above-mentioned composite bacteria liquid.
Clostridium butyricum, Lactobacillus delbrueckii in composite bacteria liquid of the present invention are the excellent species of suitable solid state fermentation feed, energy
Enough a large amount of miscellaneous bacterias, growth and breedings of harmful bacteria for effectively suppressing to contain in feeding system, feedstuff, ensure fermentation solid-state feed
Good fermentation.Aspergillus oryzae can utilize raw material to produce substantial amounts of protease, amylase, carbohydrase, cellulase, phytase
Deng the straight chain in raw material, amylopectin can be degraded to dextrin and various low molecule carbohydrates, such as maltose, glucose；Will not
Digestible macro-molecular protein is degraded to peptone, polypeptide and various amino acid, and can make crude fibre in raw material, phytic acid
The mass degradation absorbed Deng hardly possible, improves nutritive value.Globefish Bifidobacterium, is a kind of probiotics for being located away from chitling road, its for
Adjust intestinal flora balance to play a crucial role, while can effectively alleviate intestinal inflammatory and irritable bowel syndrome disease
Shape, prevents antibiotic-associated diarrhea.The growth of candida utili, which can decompose, utilizes carbohydrate synthesized micro-organism egg
In vain, substantially increase fermented feed protein level, and ferment produce a large amount of yeast dextrans have enhancing individual immunity activity,
Repair cell, the effect for improving function of intestinal canal.The harmful microorganism overwhelming majority is aerobe in enteron aisle, and institute in the present invention
Globefish Bifidobacterium, clostridium butyricum, the De Shi lactic acid bacterias used are anaerobes, and aspergillus oryzae and candida utili are
Oxygen consumption microorganism, can make oxygen depletion in enteron aisle rapidly, create favorable conditions for beneficial microbe growth.
The present invention provides a kind of preparation method of fermented feed, which mainly includes the following steps：Match somebody with somebody respectively
The matrix and composite bacteria liquid are made, the composite bacteria liquid being prepared is inoculated in matrix, is mixed, fermentation, obtains above-mentioned fermentation
Feed.
In an optional embodiment, above-mentioned matrix is counted including following component in parts by weight：50~62 parts of dregs of beans,
Such as can be but be not limited to 50 parts, 51 parts, 52 parts, 53 parts, 54 parts, 55 parts, 56 parts, 57 parts, 58 parts, 59 parts, 60 parts, 61
Part or 62 parts；8~15 parts of wheat bran, for example, can be but be not limited to 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts or
15 parts；2~8 parts of molasses, such as can be but be not limited to 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts or 8 parts；Corn flour 5~13
Part, such as can be but be not limited to 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts or 13 parts；Peanut meal 5~12
Part, such as can be but be not limited to 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts or 12 parts；2~10 parts of rapeseed dregs, example
It such as can be but be not limited to 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts；4~11 parts of corn protein powder, example
It such as can be but be not limited to 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts or 11 parts；1~8 part of soybean protein isolate, such as
It can be but be not limited to 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts or 8 parts；0.5~1.5 part of salt, such as can be but not
It is limited to 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts or 1.5 parts.
In one preferred embodiment, matrix is counted including following component in parts by weight：52 parts of dregs of beans, wheat bran 8
Part, 6 parts of molasses, 8 parts of corn flour, 8 parts of peanut meal, 5 parts of rapeseed dregs, 6 parts of corn protein powder, 6 parts of soybean protein isolate and salt 1
Part.
In an optional embodiment, the addition of composite bacteria liquid is 5~15% (v/w) in above-mentioned matrix.Such as
It can be but be not limited to 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14% or 15%.
In one preferred embodiment, the addition of composite bacteria liquid is 8% in above-mentioned matrix.
The present invention provides a kind of complete feed for including above-mentioned fermented feed.The complete feed is free of antibiotic, energy
It is enough to maintain intestinal health while without antibiotic.
The present invention provides a kind of above-mentioned composite bacteria liquid to prepare the application in improving weanling pig intestinal health feed.
The present invention provides a kind of feed for being used to improve weanling pig intestinal health, which includes above-mentioned fermentation and raises
Material.The feed can promote intestine of young pigs profitable strain to breed, and control the growth of pernicious bacteria, keep intestinal health；Piglet sends out
During raw catarrhal diarrhea, it can correct intestinal microecology balance, suppress harmful microorganism growth, protect intestinal wall chorion, and
When antidiarrheal, promote diarrhea after enteric recovery.And the solid state fermentation feed efficiency prepared is notable, feed profit can not only be improved
With rate, promote piglet growth, and can also improve and maintain its intestinal flora to balance, improve pigling immunity and premunition, and
The diarrhea of piglet is effectively improved, increases its survival rate.The fermented feed preparation process and final products antibiotic-frees of the present invention
Addition, it is green safe, it is environmental-friendly.
For the clearer explanation present invention, with reference to preferred embodiment, the present invention is described further.
Embodiment 1
A kind of composite bacteria liquid is present embodiments provided, meter includes 1 part of aspergillus oryzae, 5 parts of globefish Bifidobacterium, fourth in parts by weight
5 parts of sour 1 part of clostridium, 5 parts of Lactobacillus delbrueckii and candida utili.
Embodiment 2
A kind of composite bacteria liquid is present embodiments provided, meter includes 5 parts of aspergillus oryzae, 1 part of globefish Bifidobacterium, fourth in parts by weight
15 parts of sour 5 parts of clostridium, 1 part of Lactobacillus delbrueckii and candida utili.
Embodiment 3
Present embodiments provide a kind of composite bacteria liquid, in parts by weight meter include 1 part of aspergillus oryzae, 3.5 parts of globefish Bifidobacterium,
8 parts of 1 part of clostridium butyricum, 2.5 parts of Lactobacillus delbrueckii and candida utili.
Embodiment 4
A kind of composite bacteria liquid is present embodiments provided, meter includes 3 parts of aspergillus oryzae, 1 part of globefish Bifidobacterium, fourth in parts by weight
12 parts of sour 3 parts of clostridium, 1 part of Lactobacillus delbrueckii and candida utili.
Embodiment 5
A kind of composite bacteria liquid is present embodiments provided, meter includes 1 part of aspergillus oryzae, 2 parts of globefish Bifidobacterium, fourth in parts by weight
10 parts of sour 1 part of clostridium, 2 parts of Lactobacillus delbrueckii and candida utili.
Comparative example 1
This comparative example provides a kind of composite bacteria liquid, and meter includes 0.5 part of aspergillus oryzae, globefish Bifidobacterium 10 in parts by weight
Part, 0.5 part of clostridium butyricum, 10 parts of Lactobacillus delbrueckii and 1 part of candida utili.
Comparative example 2
This comparative example provides a kind of composite bacteria liquid, in parts by weight meter include 8 parts of aspergillus oryzae, 0.5 part of globefish Bifidobacterium,
20 parts of 7 parts of clostridium butyricum, 0.5 part of Lactobacillus delbrueckii and candida utili.
Comparative example 3
This comparative example provides a kind of composite bacteria liquid, and difference lies in composite bacteria liquid to be free of aspergillus oryzae with embodiment 1, by weight
Amount number meter includes, 2 parts of globefish Bifidobacterium, 1 part of clostridium butyricum, 10 parts of 2 parts of Lactobacillus delbrueckii and candida utili.
Comparative example 4
This comparative example provides a kind of composite bacteria liquid, and difference lies in composite bacteria liquid to be free of globefish Bifidobacterium with embodiment 1,
Meter includes 10 parts of 1 part of aspergillus oryzae, 1 part of clostridium butyricum, 2 parts of Lactobacillus delbrueckii and candida utili in parts by weight.
Comparative example 5
This comparative example provides a kind of composite bacteria liquid, and difference lies in composite bacteria liquid to be free of clostridium butyricum with embodiment 1, presses
Parts by weight meter includes 10 parts of 1 part of aspergillus oryzae, 2 parts of globefish Bifidobacterium, 2 parts of Lactobacillus delbrueckii and candida utili.
Comparative example 6
This comparative example provides a kind of composite bacteria liquid, and difference lies in composite bacteria liquid to be free of Lactobacillus delbrueckii with embodiment 1,
Meter includes 10 parts of 1 part of aspergillus oryzae, 2 parts of globefish Bifidobacterium, 1 part of clostridium butyricum and candida utili in parts by weight.
Comparative example 7
This comparative example provides a kind of composite bacteria liquid, and difference lies in composite bacteria liquid to be free of Candida utilis ferment with embodiment 1
Female bacterium, in parts by weight meter include 2 parts of 1 part of aspergillus oryzae, 2 parts of globefish Bifidobacterium, 1 part of clostridium butyricum and Lactobacillus delbrueckii.
Embodiment 6：
Present embodiments provide a kind of preparation method of fermented feed：
(1) composite bacteria liquid is prepared：
Seed liquor is made in aspergillus oryzae, globefish Bifidobacterium, clostridium butyricum, Lactobacillus delbrueckii and candida utili respectively,
Composite bacteria liquid is obtained after mixing.Seed liquid and preparation method thereof is as follows：
(a) preparation method of aspergillus oryzae seed liquor
The aspergillus oryzae strain activated is taken, the culture medium equipped with SP is seeded to, 20h, shaking table is cultivated in 37 DEG C of constant-temperature tables
Rotating speed is 200rpm, is spare seed liquor.
SP culture medium prescriptions are as follows：Soluble starch 20g/L, potassium dihydrogen phosphate 1g/L, magnesium sulfate 0.5g/L, ammonium sulfate
0.5g/L, agar 20g/L, is dissolved in soya-bean cake diffusion juice.
Soya-bean cake diffusion juice preparation method is as follows：100 grams of soya-bean cakes, add water 500ml, when immersion 4 is small, boil 3-4 it is small when, gauze
Natural filtration, takes liquid, adjusts to 5 Baume degrees.
(b) preparation method of globefish Bifidobacterium seed liquid
It is 7.0,30 DEG C of constant-temperature tables to take the globefish Bifidobacterium activated to be seeded to the culture medium equipped with TPY to adjust pH value
Middle culture 24h, shaking speed 180rpm, are spare seed liquor.
TPY culture medium prescriptions are as follows：Peptone 15g/L, dusty yeast 2g/L, glucose 20g/L, soluble starch 0.5g/
L, sodium chloride 5g/L, 5% cysteine 10ml, tomato leachate 400Ml, Tween 80 1ml, liver extracting solution 80Ml, agar
20g/L, be dissolved in distilled water.
(c) preparation method of butyrate spindle bacillus seed liquid
The clostridium butyricum activated is taken to be seeded in the RCM culture mediums that 200mL is preset, it is 7.3,37 DEG C to adjust pH values
18h, shaking speed 220rpm are cultivated in constant-temperature table, is spare seed liquor.
RCM culture medium prescriptions are as follows：Yeast extract 3g/L, beef extract 10g/L, trypsase peptone 10g/L, glucose
5g/L, soluble starch 1g/L, sodium chloride 5g/L, sodium acetate trihydrate 3g/L, cysteine hydrochloride 0.15g/L, be dissolved in steaming
Distilled water.
(d) preparation method of Lactobacillus delbrueckii seed liquor
The Lactobacillus delbrueckii activated is taken to be seeded to the culture medium equipped with MSR, adjusting pH value is 6.2,37 DEG C of constant-temperature tables
Middle culture 24h, shaking speed 220rpm, are spare seed liquor.
The culture medium prescription of MSR is as follows：Glucose 20g/L, peptone 10g/L, 10 g/L of beef extract, yeast extract
5g/L, dipotassium hydrogen phosphate 2g/L, anhydrous sodium acetate 5g/L, Triammonium citrate 2g/L, bitter salt 0.58g/L, four hydrations
Manganese sulfate 0.25g/L；Tween 80 1mL, for distilled water.
(e) preparation method of candida utili bacterium seed liquor
The candida utili bacterium activated is taken to be seeded in the culture medium equipped with pre-optimized mistake, adjusting pH value is
24h, shaking speed 122rpm are cultivated in 5.5,30 DEG C of constant-temperature tables, is spare seed liquor.
Culture medium prescription is：Peptone 30g/L, sucrose 60g/L, potassium dihydrogen phosphate 2g/L, magnesium sulfate 1.5g/L, ammonium sulfate
1g/L, is dissolved in distilled water.
(2) fermented feed is prepared：
(a) matrix is counted including following component in parts by weight：52 parts of dregs of beans, 8 parts of wheat bran, 6 parts of molasses, 8 parts of corn flour,
1 part of 8 parts of peanut meal, 5 parts of rapeseed dregs, 6 parts of corn protein powder, 6 parts of soybean protein isolate and salt.
(b) sterile sealed polyethylene plastic bag is loaded after feedstuff is mixed, the addition of composite bacteria liquid in matrix
For 8% (v/w), protease additive amount is 110U/g, and for material water volume weight ratio by 1: 0.6 proportioning, sealing fermentation, keeps temperature
37 DEG C, fermentation time 160h, after the completion of fermentation, air-dry crush under natural conditions, obtain fermented feed finished product.
Embodiment 7
Present embodiments provide a kind of complete feed：
Formula is as follows：The decortication of Xinjiang corn 48.25%, flour 13%, wheat-middlings 14%, imported fish meal 2.5%, 46%
Dregs of beans 13%, fermented feed 3%, cheese powder 1%, calcium monohydrogen phosphate 1.5%, soya-bean oil 0.8%, edible salt 0.4%, zinc oxide
0.3%th, 98% lysine 0.65%, threonine 0.1%, the tryptophan 0.5% of methionine 0.1%, 10% and premix
0.9%.
Wherein, the composition and ratio of the premix are as follows, and every kilogram contains：Copper 30000mg；Iron, 19000mg；Zinc,
18000mg；Manganese, 9600mg；Iodine, 80mg；Selenium, 70mg；Vitamin A, 800000IU；Vitamine D3,240000IU；Vitamin
E, 2400IU；Vitamin K3,480mg；Vitamin B1,150mg；Vitamin B2,480mg；Vitamin B6,240mg；Vitamin
B12,1.6mg；Nicotinic acid, 3100mg；Pantothenic acid, 1900mg；Folic acid, 100mg；Biotin, 6mg and choline chloride, 40000mg.
Comparative example 8
This comparative example provides a kind of complete feed, and difference lies in not comprising fermented feed with embodiment 7.
Comparative example 9
This comparative example provides a kind of complete feed, with embodiment 7 difference lies in not comprising fermented feed, and with the addition of
Oxytetracycline calcium (20%) 0.1% and aureomycin (15%) 0.05%.
Effect example 1:
The preparation method that embodiment 6 is respectively adopted prepares the fermentation feeding for including embodiment 1~5 and comparative example 1~7 as raw material
Material, detects soluble protein content in 1~7 fermented feed of embodiment 1~5 and comparative example, the results are shown in Table 1：
1 fermented feed content of material of table
From upper table data, fermented feed provided by the invention has the soluble protein and small molecular sugar of higher amount
Class, it is easier to absorb.Meanwhile as used in the contrast of the fermented feed of embodiment and comparative example understands fermented feed of the embodiment of the present invention
Composite bacteria liquid can by feed mesostroma fermentative degradation more fully, protein, starch and fiber is sufficiently degraded into
Soluble protein and small molecule carbohydrate.It can be seen from the above that by the cooperation between each component and complex role, can effectively improve compound
The ferment effect of bacterium solution.
Meanwhile also had for the ferment effect of composite bacteria liquid from each embodiment Contrast on effect, different raw material proportionings
Certain influence, and it is optimal according to the effect that the proportioning of embodiment 5 can reach,
Embodiment 1, embodiment 2, the proportioning of embodiment 3 and embodiment 4 can also reach good ferment effect.
Effect example 2
The growth effect of comparative example 7, comparative example 8 and comparative example 9 to weanling pig.
(1) piglet in the wean of 21 ages in days is fed, every group 16, observes the influence result such as institute of table 3 to grice diarrhoea rate
Show：
Influence of 2 fermented feed of table to diarrhea of weaned piglets rate
Note：Colleague's lowercase difference represents significant difference (P ＜ 0.05).
By Germicidal efficacy grice diarrhoea rate, fermented feed of the present invention significantly reduces 21-28,2-35,36-42
The diarrhea rate of three phases piglet, and it is known as same effect with antibiosis.
(2) embodiment 7, comparative example 8 and comparative example 9 are used respectively, are fed the piglet in the wean of 21 ages in days, every group 16, are seen
The influence to growth index is examined, as a result as shown in Table 3 and Table 4.
The influence of 3 weanling pig growth index of table
Note：Colleague's lowercase difference represents significant difference (P ＜ 0.05).
By experimental studies have found that, significantly raised 21-42 age in days stage piglet daily gains of embodiment 7, and feed efficiency
It is significantly raised.
Table 4 feeds the influence that weanling pig divides its colon interior microorganism species system
Note：Colleague's lowercase difference represents significant difference (P ＜ 0.05).
By experimental result, fermented feed can substitute antibiotics class medicine completely use, solving weanling pig should
The problem of swashing, and can effectively improve piglet immunological function, reduce morbidity and mortality, and suppress the numerous of pathogen in enteron aisle
Grow and grow, improve Bacterial community, ensure the gastrointestinal health of weanling pig, so as to improve the production performance of weanling pig and support
Grow benefit.
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe is described in detail the present invention with reference to foregoing embodiments, it will be understood by those of ordinary skill in the art that：Its according to
Can so modify to the technical solution described in foregoing embodiments, either to which part or all technical characteristic into
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
Claims (10)
1. a kind of composite bacteria liquid, it is characterised in that the composite bacteria liquid mainly includes following component：Aspergillus oryzae, Bifidobacterium, fourth
Sour clostridium, Lactobacillus delbrueckii and candida utili.
2. composite bacteria liquid according to claim 1, it is characterised in that the composite bacteria liquid is counted in parts by weight mainly to be included
Following component：1~5 part of aspergillus oryzae, 1~5 part of Bifidobacterium, clostridium butyricum 1~5,1~5 part of Lactobacillus delbrueckii and Candida utilis
5~15 parts of yeast.
3. composite bacteria liquid according to claim 2, it is characterised in that the composite bacteria liquid is counted in parts by weight mainly to be included
Following component：1~3 part of aspergillus oryzae, 1~3.5 part of Bifidobacterium, clostridium butyricum 1~3,1~2.5 part of Lactobacillus delbrueckii and production protein
8~12 parts of Candida.
4. composite bacteria liquid according to claim 3, it is characterised in that the composite bacteria liquid is counted in parts by weight mainly to be included
Following component：10 parts of 1 part of aspergillus oryzae, 2 parts of Bifidobacterium, 1 part of clostridium butyricum, 2 parts of Lactobacillus delbrueckii and candida utili.
5. a kind of fermented feed, it is characterised in that the raw material of the fermented feed is answered comprising claim 1-4 any one of them
Close bacterium solution.
6. a kind of preparation method of fermented feed, it is characterised in that the preparation method mainly includes the following steps：Prepare respectively
Matrix and the composite bacteria liquid, the composite bacteria liquid being prepared is inoculated in matrix, is mixed, fermentation, is obtained the fermentation and is raised
Material.
7. preparation method according to claim 6, it is characterised in that the matrix is counted including such as the following group in parts by weight
Point：50~62 parts of dregs of beans, 8~15 parts of wheat bran, 2~8 parts of molasses, 5~13 parts of corn flour, 5~12 parts of peanut meal, rapeseed dregs 2~
0.5~1.5 part of 10 parts, 4~11 parts of corn protein powder, 1~8 part of soybean protein isolate and salt；
Preferably, 52 parts of dregs of beans, 8 parts of wheat bran, 6 parts of molasses, 8 parts of corn flour, 8 parts of peanut meal, 5 parts of rapeseed dregs, corn protein powder 6
1 part of part, 6 parts of soybean protein isolate and salt.
8. preparation method according to claim 6, it is characterised in that the addition of composite bacteria liquid is described in the matrix
5~15% (v/w).
9. a kind of complete feed, it is characterised in that include the fermented feed described in claim 5.
10. as claim 1-4 any one of them composite bacteria liquid is preparing the feed for being used for improving weanling pig intestinal health
In application.
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| CN112352883A (en) * | 2020-11-12 | 2021-02-12 | 河南智盛优品生物科技有限公司 | Feed containing cellulase and preparation process thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392946A (en) * | 2013-08-22 | 2013-11-20 | 广南(湛江)家丰饲料有限公司 | Pig feed additive capable of effectively improving immunity and intestinal health |
| CN106819481A (en) * | 2017-02-15 | 2017-06-13 | 山东碧蓝生物科技有限公司 | A kind of full price fermented feed for piglet religion groove and preparation method thereof |
| CN107279468A (en) * | 2017-07-01 | 2017-10-24 | 曲靖双胞胎饲料有限公司 | A kind of probiotics for preventing and treating grice diarrhoea |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392946A (en) * | 2013-08-22 | 2013-11-20 | 广南(湛江)家丰饲料有限公司 | Pig feed additive capable of effectively improving immunity and intestinal health |
| CN106819481A (en) * | 2017-02-15 | 2017-06-13 | 山东碧蓝生物科技有限公司 | A kind of full price fermented feed for piglet religion groove and preparation method thereof |
| CN107279468A (en) * | 2017-07-01 | 2017-10-24 | 曲靖双胞胎饲料有限公司 | A kind of probiotics for preventing and treating grice diarrhoea |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112352883A (en) * | 2020-11-12 | 2021-02-12 | 河南智盛优品生物科技有限公司 | Feed containing cellulase and preparation process thereof |
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