CN107629949B - Microorganism handheld inoculation device based on three-point method - Google Patents
Microorganism handheld inoculation device based on three-point method Download PDFInfo
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- CN107629949B CN107629949B CN201710989098.1A CN201710989098A CN107629949B CN 107629949 B CN107629949 B CN 107629949B CN 201710989098 A CN201710989098 A CN 201710989098A CN 107629949 B CN107629949 B CN 107629949B
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- 238000011081 inoculation Methods 0.000 title claims abstract description 28
- 244000005700 microbiome Species 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 5
- 230000001954 sterilising effect Effects 0.000 abstract description 4
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 229960005486 vaccine Drugs 0.000 description 12
- 238000010586 diagram Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 208000006400 Arbovirus Encephalitis Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010052369 Encephalitis lethargica Diseases 0.000 description 1
- 206010035148 Plague Diseases 0.000 description 1
- 241000589970 Spirochaetales Species 0.000 description 1
- 208000037386 Typhoid Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 229960000190 bacillus calmette–guérin vaccine Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960005004 cholera vaccine Drugs 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229960003239 encephalitis vaccine Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229940041323 measles vaccine Drugs 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 229940066827 pertussis vaccine Drugs 0.000 description 1
- 229960001539 poliomyelitis vaccine Drugs 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 201000008297 typhoid fever Diseases 0.000 description 1
- 201000002498 viral encephalitis Diseases 0.000 description 1
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Abstract
The invention provides a three-point method-based microorganism handheld inoculation device, belonging to the technical field of microorganism inoculation; the automatic needle cylinder device comprises a shell, wherein a screw is connected to the middle of the shell in a threaded manner, the upper end of the screw is connected with a motor through a coupler, a group of symmetrical handles is arranged on the motor, a motor control switch is arranged on each handle, a movable head body is arranged at the front end of the shell, three needle cylinder shells distributed at equal intervals are arranged at the lower end of the movable head body, a piston is arranged in each needle cylinder shell, one end of a first connecting rod is connected to the piston, the other end of the first connecting rod is hinged to one end of a second connecting rod, and the other end of the; the three-point microbial inoculation can be simultaneously completed by arranging the three inoculating needles, the screw rod, the piston, the second connecting rod, the first connecting rod and the like which are arranged in an equilateral triangle, the inoculation is completed without being sterilized for multiple times in batches, and the consistent time of the inoculation and the sterilization is ensured.
Description
Technical Field
The invention discloses a three-point method-based microorganism handheld inoculation device, and belongs to the technical field of microorganism inoculation.
Background
The vaccine is a biological product which is prepared from various pathogenic microorganisms and is used for vaccination. Wherein the vaccine made with the bacteria or spirochetes is also called bacterin. Vaccines are divided into live and killed vaccines. The commonly used live vaccines include BCG vaccine, polio vaccine, measles vaccine, plague vaccine, etc. The commonly used killed vaccines include pertussis vaccine, typhoid vaccine, epidemic encephalitis vaccine, cholera vaccine and the like.
The production time of different vaccines varies, and some vaccines may take 22 months to produce a batch. Vaccine development is a long and complex process and is costly. Vaccination is the most economical and effective public health intervention for preventing and controlling infectious diseases, and is also an effective means for reducing the occurrence of member diseases and medical expenses for families.
In the current state of the art, when we are conducting microbial vaccine experiments in the laboratory, we often use the "three-point method" (this method is to inoculate a small amount of microbes on the surface of a plate, form three points of an equilateral triangle, and form the microbes independently to observe and study their morphology), to culture the microbes, and to inoculate at three fixed locations on the culture medium. Normally, if the inoculation is needed several times, the inoculation needle needs to be used for sterilization and inoculation several times respectively to complete the inoculation on a culture medium, which brings great trouble to the experimental operation and often cannot ensure the time of sterilization and inoculation to be completely consistent.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a microorganism handheld inoculation device based on a three-point method, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention is realized by the following technical scheme: a microorganism handheld inoculation device based on a three-point method comprises a shell, wherein a lead screw is in threaded connection with the middle of the shell, the upper end of the lead screw is connected with a motor through a coupler, a set of symmetrical handles is arranged on the motor, a motor control switch is arranged on the handles, a movable head body is arranged at the front end of the shell, three cylinder shells distributed in equal distance are arranged at the lower end of the movable head body, a piston is arranged in each cylinder shell, one end of a first connecting rod is connected onto the piston, the other end of the first connecting rod is hinged to one end of a second connecting rod, the other end of the second connecting rod is hinged to a movable round platform, the movable round platform is positioned below the lead screw, a fixed boss is hinged to the middle of the second connecting rod and fixed in the movable head body, one end of a spring is arranged on the second connecting, the spring is arranged between the first connecting rod and the fixing boss.
Furthermore, the handle is divided into a left handle and a right handle, the left handle is provided with a motor forward rotation control switch, and the right handle is provided with a motor reverse rotation control switch.
Further, a handheld end is arranged on the shell.
Furthermore, scale marks are arranged on the syringe shell.
The invention has the beneficial effects that: the invention has simple structure and convenient operation, can simultaneously complete three-point microorganism inoculation by arranging the three inoculating needles, the screw rod, the piston, the second connecting rod, the first connecting rod and the like in an equilateral triangle, does not need to complete the inoculation in batches and by multiple times of disinfection, and ensures the consistent time of inoculation and sterilization; through the motor and the lead screw that set up, save a lot of troubles for the staff.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a schematic structural diagram of a microorganism handheld inoculation device based on a three-point method;
FIG. 2 is a diagram of the state of the microorganism hand-held inoculation device of the invention when inoculated in the fixed head body;
FIG. 3 is a state diagram of the microorganism in the fixed head body of the microorganism handheld inoculation device based on the three-point method for sucking microorganisms;
in the figure: 1-shell, 2-fixed head body, 3-lead screw, 4-handheld end, 5-motor, 51-handle, 52-motor control switch, 6-syringe shell, 7-piston, 8-first connecting rod, 9-spring, 10-second connecting rod, 11-fixed boss and 12-movable round table.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Referring to fig. 1-3, the present invention provides a technical solution: a microorganism handheld inoculation device based on a three-point method comprises a shell 1, wherein a screw 3 is in threaded connection with the middle of the shell 1, the upper end of the screw 3 is connected with a motor 5 through a coupler, a set of symmetrical handles 51 are arranged on the motor 5, a motor control switch 52 is arranged on each handle 51, a movable head body 2 is arranged at the front end of the shell 1, a needle cylinder shell 6 with three equal-distance distribution is arranged at the lower end of the movable head body 2, a piston 7 is arranged in the needle cylinder shell 6, one end of a first connecting rod 8 is connected onto the piston 7, one end of a second connecting rod 10 is hinged to the other end of the first connecting rod 8, the other end of the second connecting rod 10 is hinged to a movable circular table 12, the movable circular table 12 is positioned below the screw 3, a fixed boss 11 is hinged to the middle of the second connecting rod 10, the second connecting rod 10 is provided with one end of a spring 9, the other end of the spring 9 is arranged on the fixed head body 2, and the spring 9 is arranged between the first connecting rod 8 and the fixed boss 11.
The handle 51 is divided into a left handle and a right handle, the left handle is provided with a motor forward rotation control switch, and the right handle is provided with a motor reverse rotation control switch.
Be provided with handheld end 4 on casing 1, make things convenient for the staff to hand.
And scale marks are arranged on the needle cylinder shell 6.
The working process is as follows: after the three syringe shells 6 are disinfected, the syringe shells are placed in a microorganism storage place, a worker places the left hand on a left handle, presses a motor forward rotation control switch 52 arranged on the left handle to drive a lead screw 3 to move downwards, under the action of a fixed boss 11, a second connecting rod 10 drives a first connecting rod 8 to move upwards to suck the microorganisms into the syringe shells 6, observes scale marks and selects a proper amount of microorganisms, stops the motor to work, places the device right above an agar plate, places the worker places the right hand on the right handle, presses a motor reverse rotation control switch 52 arranged on the right handle to drive the lead screw 3 to move upwards, drives the first connecting rod 8 to move downwards under the action of a spring 9, and inoculates the microorganisms in the three syringe shells 6 onto the agar plate at the same time to complete inoculation.
While there have been shown and described what are at present considered the fundamental principles and essential features of the invention and its advantages, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (4)
1. A handheld inoculation device of microorganism based on three point method, includes the casing, its characterized in that: the middle part of the shell is connected with a screw rod in a threaded manner, the upper end of the screw rod is connected with a motor through a coupler, the motor is provided with a group of symmetrical handles, the handle is provided with a motor control switch, the front end of the shell is provided with a movable head body, the lower end of the movable head body is provided with three needle cylinder shells distributed at equal intervals, a piston is arranged in the needle cylinder shell, one end of a first connecting rod is connected to the piston, the other end of the first connecting rod is hinged with one end of a second connecting rod, the other end of the second connecting rod is hinged on a movable round table which is positioned below the screw rod, the middle part of the second connecting rod is hinged with a fixed boss which is fixed in the movable head body, the second connecting rod is provided with one end of a spring, the other end of the spring is arranged on the fixed head body, and the spring is arranged between the first connecting rod and the fixed boss.
2. The three-point method-based microorganism handheld inoculation device is characterized in that: the handle is divided into a left handle and a right handle, a motor forward rotation control switch is arranged on the left handle, and a motor reverse rotation control switch is arranged on the right handle.
3. The three-point method-based microorganism handheld inoculation device is characterized in that: the shell is provided with a handheld end.
4. The three-point method-based microorganism handheld inoculation device is characterized in that: and scale marks are arranged on the needle cylinder shell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710989098.1A CN107629949B (en) | 2017-10-22 | 2017-10-22 | Microorganism handheld inoculation device based on three-point method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710989098.1A CN107629949B (en) | 2017-10-22 | 2017-10-22 | Microorganism handheld inoculation device based on three-point method |
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| Publication Number | Publication Date |
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| CN107629949A CN107629949A (en) | 2018-01-26 |
| CN107629949B true CN107629949B (en) | 2020-11-03 |
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| CN201710989098.1A Expired - Fee Related CN107629949B (en) | 2017-10-22 | 2017-10-22 | Microorganism handheld inoculation device based on three-point method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6374683B1 (en) * | 1999-01-29 | 2002-04-23 | Genomic Instrumentation Services, Inc. | Pipetter |
| JP2010536395A (en) * | 2007-08-30 | 2010-12-02 | エッグ チック オートメイテッド テクノロジーズ | Method of injecting therapeutic substance into eggs and corresponding injection head |
| CN203853098U (en) * | 2014-05-27 | 2014-10-01 | 广州冠科生物科技有限公司 | Full-automatic liquid transfer device |
| CN206280584U (en) * | 2016-12-07 | 2017-06-27 | 北京神雾环境能源科技集团股份有限公司 | A kind of fully closed four-way reversing valve of single cylinder-actuated |
| CN206622126U (en) * | 2017-04-13 | 2017-11-10 | 重庆生科源生物技术有限公司 | A kind of adjustable pipette |
-
2017
- 2017-10-22 CN CN201710989098.1A patent/CN107629949B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6374683B1 (en) * | 1999-01-29 | 2002-04-23 | Genomic Instrumentation Services, Inc. | Pipetter |
| JP2010536395A (en) * | 2007-08-30 | 2010-12-02 | エッグ チック オートメイテッド テクノロジーズ | Method of injecting therapeutic substance into eggs and corresponding injection head |
| CN203853098U (en) * | 2014-05-27 | 2014-10-01 | 广州冠科生物科技有限公司 | Full-automatic liquid transfer device |
| CN206280584U (en) * | 2016-12-07 | 2017-06-27 | 北京神雾环境能源科技集团股份有限公司 | A kind of fully closed four-way reversing valve of single cylinder-actuated |
| CN206622126U (en) * | 2017-04-13 | 2017-11-10 | 重庆生科源生物技术有限公司 | A kind of adjustable pipette |
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| CN107629949A (en) | 2018-01-26 |
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| CB03 | Change of inventor or designer information |
Inventor after: Wang Wenqi Inventor after: Zhang Yuna Inventor after: Xu Shenbo Inventor after: Zhao Weiqing Inventor after: Wang Kang Inventor after: Wang Bingyu Inventor before: Zhao Weiqing Inventor before: Wang Kang Inventor before: Wang Bingyu |
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Effective date of registration: 20201009 Address after: 266109 Qingdao Road, Chengyang District, Shandong, No. 700 the Great Wall Applicant after: Qingdao Agricultural University Applicant after: Qingdao Hairuo wisdom Technology Co.,Ltd. Address before: 250000 Shandong Province, Lixia District of Ji'nan City East Mountain Division of Shandong University of Traditional Chinese Medicine College of traditional Chinese Medicine Applicant before: Zhao Weiqing |
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