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CN107467670A - A kind of probiotics preparation and preparation method and application - Google Patents

A kind of probiotics preparation and preparation method and application Download PDF

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Publication number
CN107467670A
CN107467670A CN201710854857.3A CN201710854857A CN107467670A CN 107467670 A CN107467670 A CN 107467670A CN 201710854857 A CN201710854857 A CN 201710854857A CN 107467670 A CN107467670 A CN 107467670A
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probiotics preparation
probiotics
purslane
preparation
pepsin
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CN107467670B (en
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吴顺来
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    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
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    • A61K35/74—Bacteria
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
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    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/488—Aspartic endopeptidases (3.4.23), e.g. pepsin, chymosin, renin, cathepsin E
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Abstract

The present invention relates to a kind of probiotics preparation and preparation method and application, the probiotics preparation includes by mass percentage：30 45% bacillus subtilis, 5 20% bacillus licheniformis, 20 40% enterococcus faecalis, 20 35% pepsin, 0 5% purslane.The probiotics preparation can be applied in the health products, food, medicine or the feed that are used for nursing one's health enteric microorganism environment is prepared, it can effectively treat the constipation caused by intestinal flora is lacked of proper care, the probiotics preparation can also be applied in fermented feed is prepared simultaneously, and it can greatly shorten the fermentation time of fermented feed.

Description

A kind of probiotics preparation and preparation method and application
Technical field
The invention belongs to biological technical field, and in particular to a kind of probiotics preparation and preparation method and application.
Background technology
Live away from home miscellaneous microorganism in the enteron aisle of healthy humans and animals, and these microorganisms are referred to as gut flora.Intestines Road flora combines according to a certain percentage, conditions each other, depends on each other for existence between each bacterium, forms a kind of ecological balance on quality and quantity, and one Denier body internal and external environment changes, such as diet changes, using antibiotic medicine, intestinal immunity obstacle etc., leads Sensitization intestines bacterium is pressed, and not repressed bacterium seizes the opportunity breeding, so as to cause flora imbalance, human or animal is produced corresponding disease Disease.
Normal fecal stools contain 70-80% moisture content, and the holding of these moisture content just has benefited from the attachment and presence of intestinal flora. So maintaining the health of body, it is very necessary to keep enteral beneficial bacterium dominance.If there is no gut flora in enteron aisle, Such as eaten antibiotic and gut flora is largely all killed, also just without the perfect adaptation of flora and moisture content in excrement, Excrement is done not only firmly, cause constipation.
The medicine for the treatment constipation clinically commonly used at present has：Bulk cathartic, irritant laxative, emollient cathartic, ooze Permeability caccagogue, intestinal motive force medicine, probiotics etc., though effective, its side effect in treatment constipation of these medicines It is fairly obvious, there is injury to humans and animals.There are the probiotics preparation of a little treatment constipation, these probiotic products bacterium above market Number is high, but is protected due to lacking, and it is really seldom to successfully arrive at the number of viable of enteron aisle fixed point breeding, causes treatment cycle generally inclined It is long, it is impossible to the timely and effectively pain of relief of constipation patient.
The content of the invention
The present invention first purpose be to provide a kind of probiotics preparation, the probiotics preparation using probiotics suppress have Evil bacterium, can nurse one's health enteric microorganism environment, make beneficial bacterium dominance in enteron aisle, treat caused by intestinal flora is lacked of proper care just It is secret；Meanwhile make the excrement desaturation in enteron aisle by pepsin, excrement is smoothly discharged, and the excrement odorless discharged, the benefit Probiotics preparation is rapid-action, significant effect, moreover it is possible to improves the digestive function and immunologic function of human body and animal.
To achieve the above object, probiotics preparation provided by the invention, it includes by mass percentage：30-45%'s Bacillus subtilis, 5-20% bacillus licheniformis, 20-40% enterococcus faecalis, 20-35% pepsin, 0-5% Purslane.
As a preference of the present invention, probiotics preparation provided by the invention, it includes by mass percentage：30-40% Bacillus subtilis, 10-20% bacillus licheniformis, 25-35% enterococcus faecalis, 20-30% pepsin, 2- 5% purslane.
As present invention further optimization, probiotics preparation provided by the invention, it includes by mass percentage： 35% bacillus subtilis, 10% bacillus licheniformis, 30% enterococcus faecalis, 22% pepsin, 3% dent Amaranth.
As the present invention still more preferably, probiotics preparation provided by the invention when specifically used can by its with Glucide carries out compatible use, and the glucide addition is at least 3 times of probiotics preparation；The glucide is preferred The higher material of the sugar content such as brown sugar, white sugar or glucose or honey, it can also be other monose or oligosaccharides.Sugar Class material has proliferation function to bacillus subtilis, bacillus licheniformis, enterococcus faecalis, makes bacillus subtilis, lichens gemma Bacillus, enterococcus faecalis take advantage in enteron aisle, improve therapeutic effect.
Probiotics preparation provided by the invention, in every gram of microorganism group mixture, viable count is 10,000,000,000-110 hundred million, ensures benefit Probiotics preparation can produce effective adjustment effect to animal intestinal tract.
Second object of the present invention is to provide a kind of preparation method of probiotics preparation：Specifically include following steps：
(1) purslane after drying is weighed by above-mentioned mass percent, is crushed with pulverizer, sieved, obtain purslane Fine powder；
(2) purslane fine powder is mixed with bacillus subtilis, bacillus licheniformis, enterococcus faecalis, pepsin Uniformly.
Third object of the present invention is that providing a kind of probiotics preparation is preparing for nursing one's health enteric microorganism environment Health products, food, the application in medicine or feed, the probiotics preparation can improve the composition of flora in host intestine, in advance Anti- and improvement constipation, its is rapid-action, has no side effect, and the excrement odorless discharged.
Fourth object of the present invention is in providing a kind of application of probiotics preparation in fermented feed is prepared, by 3-5 The probiotics preparation of parts by weight, the water of 300-500 parts by weight treat that fermented feed is mixed with 1000-1200 parts by weight, under normal temperature Fermentation, the probiotics preparation can make feed be fermented in 16-32 hours completion, greatly shorten the feed fermentation time；Meanwhile After the forage feed animal after probiotics fermention, the nutritional ingredient in feed can all be absorbed, and animal defecation is normal, can Grow up healthy and sound, and immunity improves, and the illnesss such as aftosa will not occur.
Probiotics preparation provided by the invention in preparing fermented feed when applying, preferably by probiotics preparation and sugared compatibility Use, i.e., by the probiotics preparation of 3-5 parts by weight, the sugar of 50-100 parts by weight, 300-500 parts by weight water and 1000-1200 Parts by weight treat that fermented feed is mixed, and are fermented under normal temperature, and fermentation time can further be shortened by adding sugar, make fermentation time Completed in 12 hours, the fermentation time occupies first place in the world.Described sugar is brown sugar, white sugar, glucose eaten etc., can also It is other monose or oligosaccharides.
The function of each raw material is as follows in probiotics preparation provided by the invention：
Bacillus subtilis, special thalline is carried out promoting gemma and microcapsules coating is handled, in spore state stability inferior It is good, energy resistance to oxidation, anti-extrusion, high temperature resistant, acid and alkali-resistance, activity is kept in acid stomach, the attack of saliva and bile is resistant to, is micro- In biology can 100% through intestine and small intestine viable bacteria.Adding bacillus subtilis mainly has some following effect；One：Withered grass gemma Caused subtilin, polymyxins, nystatin, gramicidins isoreactivity material during bacillus thalli growth, these work Property material has obvious inhibitory action to the conditioned pathogen of pathogenic bacteria or autogenous infection；Meanwhile bacillus subtilis is rapid The free oxygen in environment is consumed, causes enteron aisle hypoxemia, is promoted beneficial to anaerobism bacteria growing, and produces the organic acids such as lactic acid, is reduced Enteron aisle pH value, suppress other pathogenic bacteria growings indirectly.Two：Bacillus subtilis can improve immunoglobulin and antibody level, Strengthen cellular immunity and humoral immune function, improve herd immunity；Meanwhile bacillus subtilis thalline itself synthesis alphalise starch The enzymes such as enzyme, protease, lipase, cellulase, together played with the digestive enzymes in animal body (human body) in alimentary canal Effect, improve the digestive function of body.Three：Bacillus subtilis by with competition of the pathogen to nutrient, suppress pathogen, Intestinal microecology balance is adjusted, forms biological containment.Meanwhile its metabolite lactic acid and bacteriocin are harmful with other are suppressed Bacterium maintains and ensured the stabilization of the combination of flora optimal vigor and this combination, prevent pathogenic in the effect of enteron aisle internal breeding Bacterium colonizing and invading, and suppresses pathogenic bacteria and harmful microbe growth and its toxin sticks；Four：Bacillus subtilis is to leather Blue positive and Grain-negative pathogenic bacteria have inhibitory action, and bacillus subtilis can suppress Escherichia coli and detection of Salmonella pair Caco-2 cells stick, so as to prevent its colonizing in enteron aisle；Five：There is bacillus subtilis good enteron aisle to stick spy Property.Bacillus subtilis and competition of the pathogenic bacteria to mannose specific receptor, reduce pathogenic bacteria and human body cell are sticked, So as to protect host from infection.
Bacillus licheniformis, cellular morphology and arrangement are in shaft-like, Dan Sheng, and adjustable flora imbalance reaches therapeutic purposes, can Body is promoted to produce antibacterial substance, kill pathogenic bacteria.Resistant activity material can be produced, and takes oxygen effect by force with unique biology Mechanism, the growth and breeding of pathogenic bacteria can be suppressed.In addition, bacillus licheniformis has stronger protease, lipase, amylase Activity, body can be promoted to absorb nutriment, strengthen immunity of organisms；Meanwhile promote normal physiological in enteron aisle Property anaerobic bacteria growth, adjust intestinal bacilli illness, recover function of intestinal canal.
Enterococcus faecalis, there is stronger tolerance and colonization ability in intestinal mucosa, natural antibiotics can be produced, be advantageous to body Health；The antibacterial substances such as bacteriocin can also be produced simultaneously, suppress the growth of the pathogen such as Escherichia coli and salmonella, improve intestines Road microenvironment, moreover it is possible to suppress the breeding of urease-producing bacterium and spoilage organisms in enteron aisle, reduce enteron aisle urease and endotoxic contain Amount, decline ammonia and endotoxic content in blood.Enterococcus faecalis is used cooperatively with bacillus subtilis and bacillus licheniformis, Bacillus subtilis and bacillus licheniformis have the function that promotion enterococcus faecalis propagation, further enhance therapeutic effect.
Pepsin, it is a kind of digestibility pepsin, by the gastric chief cells (gastric in stomach Chiefcell it is) secreted, can be small fragments of peptides by the breaks down proteins in food, pepsin is a kind of acid stomach cardia Enzyme, the peptide bond that can be formed by aromatic amino acid or acidic amino acid in decomposing protein, therefore casein, ball egg can be catalyzed In vain, histone, the keratin in horn, nail and feather, and the hydrolysis of catalysis vegetable protein etc..Added in the present invention Pepsin mainly has following two purposes；One：Nutrient is provided for bacillus subtilis, bacillus licheniformis, enterococcus faecalis, is made More through enteron aisles of viable bacterias, promote the growth of beneficial bacterium in enteron aisle.Two：Food is promoted to make in enteron aisle in stomach decomposition and inversion Excrement desaturation, aid in intestinal movement, reduce the chance that is contacted with noxious material of enteron aisle, suppress absorption and the promotion of harmful substance Excretion.
Purslane, it is cold in nature, enter the heart, liver, spleen, large intestine channel, there is clearing heat and detoxicating, sharp water clearing damp, blood-stanching and blood-cooling, anti-inflammatory analgetic And other effects, the immunity of body can also be strengthened, so as to improve the regulating power of body, Virus entry human body is avoided, in withered grass Purslane is added in bacillus, bacillus licheniformis, enterococcus faecalis, pepsin, because purslane can make enteron aisle more Lubrication, make excrement excretion more unobstructed, can greatly shorten the time for the treatment of constipation, the pain of timely relief of constipation person, its Make the excrement odorless of discharge with pepsin synergy.
The advantages of the present invention：
(1) probiotics preparation provided by the invention is by adding bacillus subtilis, bacillus licheniformis, enterococcus faecalis, And its ratio is adjusted, it can nurse one's health enteric microorganism environment, improve the composition of flora in host intestine, utilize benefit Raw bacterium suppresses harmful bacteria, can quickly adjust the constipation caused by intestinal flora is lacked of proper care.
(2) probiotics preparation provided by the invention is on the one hand bacillus subtilis, lichens bud by adding pepsin Spore bacillus, excrement intestines intestines bacterium provide nutrition, more viable bacterias go directly enteron aisle, on the other hand by pepsin acceleration food in stomach The decomposition and inversion in portion, the digestive function of body is improved, make the excrement desaturation in enteron aisle, aid in intestinal movement, further improved just It is secret.
(3) probiotics preparation provided by the invention is by adding purslane toxin expelling, fall fire, while increases the lubrication of enteron aisle Property, it is used cooperatively with bacillus licheniformis, enterococcus faecalis, pepsin, makes excrement excretion more unobstructed, shortens treatment constipation Time, synergy makes the excrement odorless of discharge with pepsin for it.
(4) probiotics provided by the invention passes through bacillus subtilis, bacillus licheniformis, enterococcus faecalis, pepsin With the effect of purslane, it adjusts the positive effect of intestinal flora imbalance, a variety of probiotics can be made to be colonized in enteron aisle, adjusts People balances with microbial population of animal intestinal tract, so as to improve the immunity of body and microbial environment degree, can effectively treat because of intestines Constipation caused by flora imbalance, rapid-action in road, and the cycle for treating constipation is shorter, moreover it is possible to the immunity of body itself is improved, from Fundamentally solves constipation.
(5) when probiotics provided by the invention is used cooperatively with sugar, it is possible to increase the activation efficiency of probiotics, be used in The fermentation time of feed can be greatly shortened in fermented feed, feed original fermentation time was shortened to 12-32 hours by 28 days；Together When, after using the forage feed animal of the probiotics fermention, the nutritional ingredient in feed can all be absorbed, the body of animal In order, the speed of growth is fast, and immunity improves, and does not have an injection and does not take medicine, must not aftosa.
Embodiment
To make those skilled in the art be better understood from the purpose, technical scheme and advantage that the present invention is implemented, with reference to Specific implementation case describes while clear, completion is carried out to technical scheme, but is not used to the scope of the present invention Restriction.
Embodiment 1
The probiotics preparation that the present embodiment provides, includes by mass percentage：Bacillus subtilis 35%, lichens gemma Bacillus 10%, enterococcus faecalis 35%, pepsin 20%, wherein, in every gram of probiotics preparation, viable count is 10,800,000,000.
Specific preparation method：The purslane after drying is weighed by above-mentioned mass percent, is crushed with pulverizer, mistake Sieve, obtains purslane fine powder；Purslane fine powder and bacillus subtilis, bacillus licheniformis, enterococcus faecalis, pepsin are entered again Row is well mixed.
Embodiment 2
The probiotics preparation that the present embodiment provides, includes by mass percentage：Bacillus subtilis 30%, lichens gemma Bacillus 20%, enterococcus faecalis 25%, pepsin 20%, purslane 5%, wherein, in every gram of probiotics preparation, viable count is 10500000000.
Specific preparation method：With embodiment 1.
Embodiment 3
The probiotics preparation that the present embodiment provides, includes by mass percentage：Bacillus subtilis 40%, lichens gemma Bacillus 5%, enterococcus faecalis 23%, pepsin 30%, purslane 2%, wherein, in every gram of probiotics preparation, viable count 101 Hundred million.
Specific preparation method：With embodiment 1.
Embodiment 4
The probiotics preparation that the present embodiment provides, includes by mass percentage：Bacillus subtilis 45%, lichens gemma Bacillus 10%, enterococcus faecalis 20%, pepsin 20%, purslane 5%, wherein, in every gram of probiotics preparation, viable count is 10500000000.
Specific preparation method：With embodiment 1.
Embodiment 5
The probiotics preparation that the present embodiment provides, includes by mass percentage：Bacillus subtilis 30%, lichens gemma Bacillus 10%, enterococcus faecalis 20%, pepsin 35%, purslane 5%, wherein, in every gram of probiotics preparation, viable count is 10000000000.
Specific preparation method：With embodiment 1.
Embodiment 6
The probiotics preparation that the present embodiment provides, includes by mass percentage：Bacillus subtilis 30%, lichens gemma Bacillus 5%, enterococcus faecalis 40%, pepsin 22%, purslane 3%, wherein, in every gram of probiotics preparation, viable count 107 Hundred million.
Specific preparation method：With embodiment 1.
Embodiment 7
The probiotics preparation that the present embodiment provides, includes by mass percentage：Bacillus subtilis 35%, lichens gemma Bacillus 10%, enterococcus faecalis 30%, pepsin 22%, purslane 3%, wherein, in every gram of probiotics preparation, viable count is 10700000000.
Specific preparation method：With embodiment 1.
Comparative example 1
A kind of probiotics preparation, includes by mass percentage：Bacillus subtilis 45%, bacillus licheniformis 20%, The pepsin of enterococcus faecalis 20%, 15%, wherein, in every gram of probiotics preparation, viable count is 11,000,000,000.
Comparative example 2
A kind of probiotics preparation, includes by mass percentage：Bacillus subtilis 45%, lichens gemma bar 10%, excrement Enterococcus 30%, pepsin 12%, purslane 3%, wherein, in every gram of probiotics preparation, viable count is 11,000,000,000.
The applicable cases of probiotics preparation of the present invention clinically are as follows：
1st, case selection：
This group of case be because intestinal flora lack of proper care caused by constipation patient, meet include, exclusion standard, the age Between 20-65 year, wherein, man accounts for 50%, Ms and accounts for 50%.It is randomly divided into 12 groups, every group each 20, wherein experimental group is the 1~9 group, control group is 10-12 groups.There was no significant difference for the data such as age of this 12 groups of patients, sex, the state of an illness, and having can Compare property.
2nd, therapeutic scheme：
1st~7 group of (experimental group) patient takes the preparation that embodiment 1~7 is provided respectively, once a day, each 1.5g； 8th group (experimental group), the preparation that the 9th group (experimental group) takes embodiment 6 respectively, embodiment 7 is provided, and after preparation is taken Take 5g white sugar in addition, the preparation that the 10th group of (control group) edible for patients comparative example 1 is provided, once a day, and each 1.5g, The preparation that 11 groups of (control group) edible for patients comparative examples 1 are provided, once a day, each 1.5g, and after preparation is taken in addition Take 5g white sugar；The preparation that 12nd group of (control group) edible for patients comparative example 2 is provided, once a day, each 1.5g.
Observe and record：After medication 7 days, record the state of an illness after 15 days, after 30 days respectively, and according to " significant effect ", " effective ", engineering noise these three grades are evaluated come the therapeutic action to the medicament.
3rd, clinical evaluation standard：
Significant effect：Patient defecates 1-2 times/day, and just matter is soft, odorless, does not glue urinal.
Effectively：Patient defecates 2 once, and just matter need to continue to take medicine close to normally.
It is invalid：Patient defecates situation without significant change.
4th, observed after taking 7 days, as a result as shown in table 1：
The clinical test results of table 1
Project Significant effect (example) Effectively (example) Invalid (example)
Experimental group 1 13 7 0
Experimental group 2 17 3 0
Experimental group 3 17 3 0
Experimental group 4 18 2 0
Experimental group 5 16 4 0
Experimental group 6 17 3 0
Experimental group 7 18 2 0
Experimental group 8 19 1 0
Experimental group 9 19 1 0
Control group 1 0 6 14
Control group 2 0 8 12
Control group 3 2 9 9
5th, observed after taking 15 days, as a result as shown in table 2：
The clinical test results of table 2
Project Significant effect (example) Effectively (example) Invalid (example)
Experimental group 1 16 4 0
Experimental group 2 19 1 0
Experimental group 3 18 2 0
Experimental group 4 19 1 0
Experimental group 5 18 2 0
Experimental group 6 19 1 0
Experimental group 7 20 0 0
Experimental group 8 20 0 0
Experimental group 9 20 0 0
Control group 1 1 9 10
Control group 2 1 11 8
Control group 3 5 10 5
6th, observed after taking 30 days, as a result as shown in table 3：
The clinical test results of table 3
It can be seen from the results above that the addition of pepsin is directly affected and controlled in probiotics preparation provided by the invention Therapeutic effect, when pepsin addition is too low, its therapeutic effect is poor, even if long-term use of, effect is still undesirable.Meanwhile Purslane is added in said preparation can increase therapeutic effect, hence it is evident that shorten treatment cycle；And the probiotics preparation and white sugar are same When taking, it can further strengthen therapeutic effect.
As can be seen here, probiotics preparation provided by the invention can prepare the health care for nursing one's health enteric microorganism environment Application in product, food, medicine or feed, improving the composition of flora in host intestine, prevention and improvement constipation, its is rapid-action, Have no side effect, and the excrement odorless discharged.
Embodiment 8
Application of the probiotics preparation produced by the present invention in fermented feed is prepared.
1000 parts by weight agriculture grain crop (potato 20-25%, corn 18-20%, dregs of beans 5-10%, bone meal 2-5%, Bean dregs 34-40%, vegetables 4-6%, melon seedling 7-10%) in add 3 parts by weight of example 7 made from probiotics preparation, 300 weights The water of part is measured, is fermented under normal temperature state, can be prepared by fermented feed within 20 hours.
Embodiment 9
1000 parts by weight agriculture grain crop (potato 20-25%, corn 18-20%, dregs of beans 5-10%, bone meal 2-5%, Bean dregs 34-40%, vegetables 4-6%, melon seedling 7-10%) in add 3 parts by weight embodiment 7 made from probiotics preparation, 300 The white sugar of the water of parts by weight, 50 parts by weight, ferment under normal temperature state, can be prepared by fermented feed within 12 hours.
By fermented feed feed for nursing pig made from embodiment 8, embodiment 9, feed pig does not have sick and raw in three months Long the nutritional ingredient utilization rate in feed is high in order, eats the fermented feed and is growed compared with the pig of common fermentation forage feed Faster, and the immunity of feed pig improves, and aftosa during which must not be crossed, also must cross other illnesss.

Claims (10)

  1. A kind of 1. probiotics preparation, it is characterised in that：Include by mass percentage：30-45% bacillus subtilis, 5-20% Bacillus licheniformis, 20-40% enterococcus faecalis, 20-35% pepsin, 0-5% purslane.
  2. A kind of 2. probiotics preparation according to claim 1, it is characterised in that：Include by mass percentage：30-40% Bacillus subtilis, 10-20% bacillus licheniformis, 25-35% enterococcus faecalis, 20-30% pepsin, 2-5% Purslane.
  3. A kind of 3. probiotics preparation according to claim 1, it is characterised in that：Include by mass percentage：35% it is withered Careless bacillus, 10% bacillus licheniformis, 30% enterococcus faecalis, 22% pepsin, 3% purslane.
  4. A kind of 4. probiotics preparation according to claim 1, it is characterised in that：Viable count is in every gram of probiotics preparation 10000000000-110 hundred million.
  5. 5. the preparation method of the probiotics preparation described in claim 1, it is characterised in that：Specifically include following steps：
    （1）The purslane after drying is weighed on request, is crushed with pulverizer, is sieved, is obtained purslane fine powder；
    （2）Purslane fine powder is well mixed with bacillus subtilis, bacillus licheniformis, enterococcus faecalis, pepsin .
  6. 6. the probiotics preparation described in claim 1 or 2 or 3 is preparing the health products for being used to nurse one's health enteric microorganism environment, eaten Application in product, medicine or feed.
  7. 7. a kind of probiotics preparation according to claim 6 prepare be used for nurse one's health enteric microorganism environment health products, Application in food, medicine or feed, it is characterised in that：It is described by probiotics preparation and glucide compatibility when specifically used Glucide addition is at least 3 times of probiotics preparation.
  8. 8. application of the probiotics preparation described in claim 1 or 2 or 3 in fermented feed is prepared.
  9. 9. application of the probiotics preparation according to claim 8 in fermented feed is prepared, it is characterised in that：It is specifically used When water and the 1000-1200 parts by weight of the probiotics preparation of 3-5 parts by weight, 300-500 parts by weight are treated that fermented feed mixes , fermented under normal temperature.
  10. 10. application of the probiotics preparation according to claim 9 in fermented feed is prepared, it is characterised in that：Specifically make Used time by probiotics preparation and glucide compatible use, by the probiotics preparation of 3-5 parts by weight, the sugar of 50-100 parts by weight Class material, water and the 1000-1200 parts by weight of 300-500 parts by weight treat that fermented feed is mixed, and ferment under normal temperature.
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