CN106171875A - A kind of cultivated strawberry substrate with Chinese medicine slag as primary raw material - Google Patents
A kind of cultivated strawberry substrate with Chinese medicine slag as primary raw material Download PDFInfo
- Publication number
- CN106171875A CN106171875A CN201610499527.2A CN201610499527A CN106171875A CN 106171875 A CN106171875 A CN 106171875A CN 201610499527 A CN201610499527 A CN 201610499527A CN 106171875 A CN106171875 A CN 106171875A
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- chinese medicine
- medicine slag
- slag
- appropriate
- substrate
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C1/00—Ammonium nitrate fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of cultivated strawberry substrate with Chinese medicine slag as primary raw material, be prepared by the raw materials in: Chinese medicine slag 45 48, Bombyx mori L. 13 14, durian shell 8 10, ammonium nitrate 58, potassium chlorate 34, alimentary acetic acid 23, charcoal powder 45, sunflower plate granulated slag 22 25, Periostracum Serpentis waste material 32 34, pepsin preparation 1.5 1.7,1 (3 dimethylamino-propyl) 3 ethyl-carbodiimide hydrochloride 1.2 1.5, N N-Hydroxysuccinimide 0.4 0.5,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate；The culture medium mass-energy of the present invention consumes production waste material Chinese medicine slag in a large number, improve the reuse ratio of waste resource, it is simultaneous for the concrete nutrient demand of Fructus Fragariae Ananssae, the content of organic matter of cultivation matrix is significantly supplemented by techniques such as Chinese medicine slag fermentations, to meet the growth needs in the Fructus Fragariae Ananssae time of infertility, save substantial amounts of fertilizers input, improve the productivity effect of strawberry cultivating.
Description
Technical field
The present invention relates to nutrient matrix technical field, particularly relate to a kind of cultivated strawberry base with Chinese medicine slag as primary raw material
Matter.
Background technology
Substrate culture is a kind of important cultural method in the current agriculture of China, woods, flower planting industry, is that one passes through base
Matter absorbs nutritional solution and a kind of Soilless Culture Methods of oxygen, owing to it is low to soil environment requirement, equipment simple, water-saving saves fertilizer etc.
Advantage, is adopted application by vast plant development person.And continuous along with the development of China's plant husbandry and cultivated area
Expanding, the usage amount of cultivation matrix the most constantly increases, and traditional uses the single raw material such as the peat composed of rotten mosses, Vermiculitum or be simply mixed preparation
Substrate can not meet the demand of plantation, and the universal nutrition of present stage substrate simultaneously is single, causes sending out of plant in cultivation
Bud rate is low, survival rate is low, the physical instability of substrate during simultaneously using, and water absorption is poor, easily subside, surcharge
Low, it is impossible to realize higher using value for specific environment, affect the growth of plantation economy benefit.
Collagen protein, as a kind of biomacromolecule, has good pore structure after being modified, the most raw
Thing degradability, mechanical strength and biocompatibility, have good cell growth interface, can become a kind of cultivation matrix and build
In quality raw materials.China Serpentis aboundresources simultaneously, owing to the decorative pattern of Periostracum Serpentis outward appearance is the most gorgeous, pattern is unique, is used for making
The artware of various finenesses, but due to the backwardness of Periostracum Serpentis Technology, the course of processing creates a large amount of waste material, account for complete
The 20-30% of Periostracum Serpentis, if not being effectively treated, not only polluting environment, also wasting substantial amounts of raw material, containing substantial amounts of in Periostracum Serpentis
Collagen protein, this also applies for it and provides cheap raw material basis in substrate preparation.
The application for a patent for invention of Publication No. CN1702061A discloses the full nutrient matrix of a kind of botanical seedling culturing and cultivation,
This nutrient matrix use weight ratio be the peat of 50-70%, the perlite of 20-25%, the Vermiculitum of 10-15 and active substance,
Nutrient substance mixes, and has more comprehensively nutrients structure, but the cultivation matrix of this disclosure of the invention is due to size not
One, easily cause sedimentation after watering, cause the blocking of air in substrate, the crude fiber content in substrate is low simultaneously, needs extra battalion
Support the interpolation of material, improve production cost, and not for the additional use value of the real needs raising substrate cultivated, limit
Make the scale application of product.
Summary of the invention
The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of Fructus Fragariae Ananssae with Chinese medicine slag as primary raw material
Cultivation matrix.
The present invention is achieved by the following technical solutions:
A kind of cultivated strawberry substrate with Chinese medicine slag as primary raw material, is prepared by the raw materials in: Chinese medicine slag 45-
48, Bombyx mori L. 13-14, durian shell 8-10, ammonium nitrate 5-8, potassium chlorate 3-4, alimentary acetic acid 2-3, charcoal powder 4-5, sunflower plate powder
Slag 22-25, Periostracum Serpentis waste material 32-34, pepsin preparation 1.5-1.7,1-(3-dimethylamino-propyl)-3-ethyl carbodiimide salt
Hydrochlorate 1.2-1.5, N-hydroxy-succinamide 0.4-0.5,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is fitted
Amount, water are appropriate.
Specifically comprising the following steps that of the described cultivated strawberry Matrix formulation procedure with Chinese medicine slag as primary raw material
(1) Chinese medicine slag, Bombyx mori L., durian shell mixing are placed in fermentation vat, add water to the 75-of ferment heap maximum water holding capacity
80%, ferment 23-25 days, by fermentation material squeeze and filter after having fermented, obtain fermentation liquid and ferment slag is standby, simultaneously by sunflower plate
Cut off, pulverize as smalls standby；
(2) 4% sodium chloride solution is added by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour
Centrifugal 12-15 minute with 5800-6200 rev/min, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with
0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C,
Insulation is extracted 18-20 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 10 minutes, then is centrifuged with 5000-5500 rev/min
10-12 minute, collect the supernatant, obtain collagen solution standby；
(3) in step 1 gained fermentation liquid, add ammonium nitrate, potassium chlorate, alimentary acetic acid, be heated to 55-60 DEG C, reaction
After 25-30 minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, charcoal powder mixing for standby use；
(4) step 2 gained collagen solution is joined in step 3 gained material, is placed under 4-5 DEG C of low temperature environment,
After stirring 5-10 minute add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide with
And other leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-35 DEG C of drying, obtains substrate of the present invention.
The invention have the advantage that
Substrate of the present invention is by the extraction to Periostracum Serpentis, and by having reduced immunogenicity, the collagen protein of biodegradability should
Use in the preparation of cultivation matrix, utilize the mechanical performance of cross-linking modified raising albumen, it is achieved its support in substrate builds
Effect, can not only provide certain support and growth interface, the collagenous fiber bundle phase within collagen protein simultaneously for seed seedling
Be staggered porous network structure mutually that formed, is also beneficial to beneficial microorganism and carries and the extension of root cell, and is
It provides sufficient oxygen and nutrient substance, promotes the growth of plant, and culture matrix plays fixation, convenient transport.
The culture medium mass-energy of the present invention consumes production waste material Chinese medicine slag in a large number, improves the reuse ratio of waste resource, simultaneously
For the concrete nutrient demand of Fructus Fragariae Ananssae, significantly supplemented the content of organic matter of cultivation matrix by techniques such as Chinese medicine slag fermentations, with full
The growth needs in foot Fructus Fragariae Ananssae time of infertility, saves substantial amounts of fertilizers input, improves the productivity effect of strawberry cultivating.
Detailed description of the invention
A kind of cultivated strawberry substrate with Chinese medicine slag as primary raw material, is made up of the raw material of following weight portion (kg): Chinese medicine
Slag 45, Bombyx mori L. 13, durian shell 8, ammonium nitrate 5, potassium chlorate 3, alimentary acetic acid 2, charcoal powder 4, sunflower plate granulated slag 22, Periostracum Serpentis waste material
32, pepsin preparation 1.5,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride 1.2, N N-Hydroxysuccinimide
0.4,4% sodium chloride solution is appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate.
Specifically comprising the following steps that of the described cultivated strawberry Matrix formulation procedure with Chinese medicine slag as primary raw material
(1) Chinese medicine slag, Bombyx mori L., durian shell mixing are placed in fermentation vat, add water to the 75% of ferment heap maximum water holding capacity, send out
Ferment 23 days, by fermentation material squeeze and filter after having fermented, obtains fermentation liquid and ferment slag is standby, cut off by sunflower plate simultaneously, pulverize
Standby for smalls；
(2) add 4% sodium chloride solution by 1:16g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6 hours with
5800 revs/min are centrifuged 12 minutes, take precipitation clear water and clean and dry at 30 DEG C, then will precipitation and 0.15mol/L citric acid
Solution presses 1:8g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30 DEG C, and insulation is extracted 18 hours, after completing
It is heated to 85 DEG C, is incubated 10 minutes, then be centrifuged 10 minutes with 5000 revs/min, collect the supernatant, obtain collagen solution
Standby；
(3) in step 1 gained fermentation liquid, add ammonium nitrate, potassium chlorate, alimentary acetic acid, be heated to 55 DEG C, react 25 points
After clock cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, charcoal powder mixing for standby use；
(4) step 2 gained collagen solution is joined in step 3 gained material, be placed under 4 DEG C of low temperature environments, stir
After mixing 5 minutes add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N N-Hydroxysuccinimide and other
Leftover materials, cross-linking reaction 8 hours, after completing, material is warming up to 30 DEG C of drying, obtains substrate of the present invention.
In order to further illustrate the using value of the present invention, inventor have chosen respectively substrate of the present invention and commercially available commonly
Culture matrix carries out the cultivating and growing of Fructus Fragariae Ananssae, and during plantation, management method is identical, and during plantation, the physical and chemical index to substrate is carried out
Detection, result is as follows:
| Full nitrogen (%) | Full phosphorus (%) | Full potassium (%) | Total porosity (%) | Saturation moisture content (%) | |
| Substrate of the present invention | 0.982 | 0.12 | 0.44 | 89.29 | 823 |
| Commercially available generic media | 0.747 | 0.08 | 0.40 | 85.64 | 463 |
Substrate the most of the present invention plantation gained Yield of Strawberry relatively generic media improves more than 10%, it can be seen that the present invention
Substrate relatively generic media is the most sufficient, simultaneously stable in physicochemical property, and structure is excellent, has preferable prospect of the application.
Claims (2)
1. the cultivated strawberry substrate with Chinese medicine slag as primary raw material, it is characterised in that be prepared by the raw materials in:
Chinese medicine slag 45-48, Bombyx mori L. 13-14, durian shell 8-10, ammonium nitrate 5-8, potassium chlorate 3-4, alimentary acetic acid 2-3, charcoal powder 4-5, to
Day certain herbaceous plants with big flowers dish granulated slag 22-25, Periostracum Serpentis waste material 32-34, pepsin preparation 1.5-1.7,1-(3-dimethylamino-propyl)-3-ethyl carbon
Diimmonium salt hydrochlorate 1.2-1.5, N-hydroxy-succinamide 0.4-0.5,4% sodium chloride solution are appropriate, 0.15mol/L citric acid
Solution is appropriate, water is appropriate.
2. according to the cultivated strawberry substrate with Chinese medicine slag as primary raw material described in claims 1, it is characterised in that preparation side
Specifically comprising the following steps that of method
(1) Chinese medicine slag, Bombyx mori L., durian shell mixing are placed in fermentation vat, add water to the 75-80% of ferment heap maximum water holding capacity, send out
Ferment 23-25 days, by fermentation material squeeze and filter after having fermented, obtains fermentation liquid and ferment slag is standby, is cut off by sunflower plate, powder simultaneously
Broken standby for smalls；
(2) add 4% sodium chloride solution by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour with
5800-6200 rev/min is centrifugal 12-15 minute, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with
0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C,
Insulation is extracted 18-20 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 10 minutes, then is centrifuged with 5000-5500 rev/min
10-12 minute, collect the supernatant, obtain collagen solution standby；
(3) in step 1 gained fermentation liquid, add ammonium nitrate, potassium chlorate, alimentary acetic acid, be heated to 55-60 DEG C, react 25-30
After minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, charcoal powder mixing for standby use；
(4) step 2 gained collagen solution is joined in step 3 gained material, be placed under 4-5 DEG C of low temperature environment, stirring
After 5-10 minute add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide and its
His leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-35 DEG C of drying, obtains substrate of the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610499527.2A CN106171875A (en) | 2016-06-28 | 2016-06-28 | A kind of cultivated strawberry substrate with Chinese medicine slag as primary raw material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610499527.2A CN106171875A (en) | 2016-06-28 | 2016-06-28 | A kind of cultivated strawberry substrate with Chinese medicine slag as primary raw material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106171875A true CN106171875A (en) | 2016-12-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610499527.2A Pending CN106171875A (en) | 2016-06-28 | 2016-06-28 | A kind of cultivated strawberry substrate with Chinese medicine slag as primary raw material |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111011192A (en) * | 2019-12-27 | 2020-04-17 | 贵州百灵企业集团制药股份有限公司 | Planting method for potted strawberries on elevated seedbed |
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| CN102716516A (en) * | 2012-05-11 | 2012-10-10 | 天津大学 | Polydatin modified collagen scaffold, and preparation method and application thereof |
| CN103159967A (en) * | 2013-04-11 | 2013-06-19 | 江南大学 | Preparation method of collagen-based sponge wound dressing with self-anti-inflammatory function |
| CN103627761A (en) * | 2013-10-22 | 2014-03-12 | 浙江省海洋开发研究院 | Method for preparing collagen peptide rich in hydroxyproline |
| CN103961749A (en) * | 2014-05-07 | 2014-08-06 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein/silica membrane double-layer stent |
| CN104447092A (en) * | 2014-12-03 | 2015-03-25 | 大新县生产力促进中心 | Special composite fertilizer for broadleaf holly leaves and production method of composite fertilizer |
| CN104825385A (en) * | 2015-04-22 | 2015-08-12 | 天津大学 | Collagen-based nanometer silver antibacterial support and its preparation method and use |
| CN105418187A (en) * | 2014-09-20 | 2016-03-23 | 南京新世纪园艺研究所 | Chinese herb residue strawberry cultivating organic fertilizer and preparation method thereof |
-
2016
- 2016-06-28 CN CN201610499527.2A patent/CN106171875A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102716516A (en) * | 2012-05-11 | 2012-10-10 | 天津大学 | Polydatin modified collagen scaffold, and preparation method and application thereof |
| CN103159967A (en) * | 2013-04-11 | 2013-06-19 | 江南大学 | Preparation method of collagen-based sponge wound dressing with self-anti-inflammatory function |
| CN103627761A (en) * | 2013-10-22 | 2014-03-12 | 浙江省海洋开发研究院 | Method for preparing collagen peptide rich in hydroxyproline |
| CN103961749A (en) * | 2014-05-07 | 2014-08-06 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein/silica membrane double-layer stent |
| CN105418187A (en) * | 2014-09-20 | 2016-03-23 | 南京新世纪园艺研究所 | Chinese herb residue strawberry cultivating organic fertilizer and preparation method thereof |
| CN104447092A (en) * | 2014-12-03 | 2015-03-25 | 大新县生产力促进中心 | Special composite fertilizer for broadleaf holly leaves and production method of composite fertilizer |
| CN104825385A (en) * | 2015-04-22 | 2015-08-12 | 天津大学 | Collagen-based nanometer silver antibacterial support and its preparation method and use |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111011192A (en) * | 2019-12-27 | 2020-04-17 | 贵州百灵企业集团制药股份有限公司 | Planting method for potted strawberries on elevated seedbed |
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| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhang Dianxing Inventor after: Huo Mingqin Inventor after: Qian Xiaoqiang Inventor after: Lan Wei Inventor after: Zhang Haitao Inventor before: Zhang Dianxing Inventor before: Huo Mingqin |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161207 |
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| RJ01 | Rejection of invention patent application after publication |