CN106177920A - Adipose tissue preparation for promoting wound healing and preparation method thereof - Google Patents
Adipose tissue preparation for promoting wound healing and preparation method thereof Download PDFInfo
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- CN106177920A CN106177920A CN201610614779.5A CN201610614779A CN106177920A CN 106177920 A CN106177920 A CN 106177920A CN 201610614779 A CN201610614779 A CN 201610614779A CN 106177920 A CN106177920 A CN 106177920A
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- fatty tissue
- preparation
- wound healing
- breviscapine
- tissue preparation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
- A61K31/728—Hyaluronic acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/35—Fat tissue; Adipocytes; Stromal cells; Connective tissues
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Dermatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
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Abstract
The invention relates to the technical field of biomedicine, in particular to an adipose tissue preparation for promoting wound healing.
Description
Technical field
The present invention relates to fatty tissue preparation technique field, be specifically related to a kind of fatty tissue preparation promoting wound healing
And preparation method thereof.
Background technology
Due to reasons such as wound, acne, burns, making skin produce wound surface, skin wound healing is that skin histology is damaged
Repair process after wound, repair process includes that inflammatory reaction, hyperplasia and cambium are reinvented.Wound for larger area
Wound, traditional skin flap transplantation technology also exists and selects and the problem of damage for district, and the repairing effect that finally can reach is also
Unsatisfactory, the skin color of transplanting often differs relatively big with surrounding skin, the most postoperative contracture that is susceptible to, tractive week
Enclose histoorgan deformation or cause dysfunction.The birth of autologous fat transplantation technology largely solves above asking
Topic, and at a lot of aspects, there is many unrivaled advantages, as district is wide, easy and simple to handle, plasticity is effective；But,
Become technical barrier at the postoperative tissue survival of fat transplantation, have that survival rate is low, easily the shortcoming such as be absorbed by organisms, it is impossible to meet
It is actually needed.
Hyaluronic acid has another name called hyaluronic acid, is a kind of acidic mucopolysaccharide, can formed and has glutinous bullet after water molecules
The material of property.It is primarily present in human body in skin and connective tissue, can be as a kind of cell base providing cell to imbed
Metallic substance, in addition to providing the epimatrix of certain volume, it is also possible to affect the stability of tissue, adhesion and glue for cells in vivo
Elastic.The molecular structure of all Natural hyaluronic acid is just as, almost without between material and tissue-specific difference,
So pure hyaluronic acid does not has immunogenicity.
Fibronectin is also referred to as fibronectin (Fibronectin, FN), is a kind of high of foreign study discovery in 1974
Glycoproteins, has various biological function.FN is widely present in animal tissue and tissue fluid, has multiple biological alive
Property.A large amount of results of study both domestic and external prove, FN molecule conservative during evolution is very strong, the FN tool in various animal body fluids
There is the most close structure, character and biological function, thus the FN of separate sources can be substituted for each other use.FN major function
Being mediated cell adhesion, the fibronectin of purification can strengthen the adhesion of cell adhesion and cell and substrate.By adhesion, fibre is even
By the shape of cellular signal transduction pathways regulation cell and the tissue of cytoskeleton, albumen can promote that cell is sprawled.At embryo
During fetal hair (Crinis Carbonisatus) is raw, fibronectin is required for migration and the differentiation of many cell types.In repair in trauma, fine even egg
White is also important.In blood clot forming process, fibronectin promotes that platelet is attached to vascular injury position.
Summary of the invention
The present invention is directed to problems of the prior art, it is provided that a kind of fatty tissue preparation promoting wound healing, no
Only fatty tissue is had bigger protective effect, and the healing of wound surface can be had preferable facilitation, cicatrix is formed
Preferably control action.
The present invention also provides for the preparation method of a kind of fatty tissue preparation promoting wound healing.
The present invention is achieved through the following technical solutions this purpose:
A kind of fatty tissue preparation promoting wound healing, the component including including following concentration:
As preferably, the consumption of described fatty tissue is 1～5mL.
As preferably, the size of institute's fatty tissue granule is 0.2～0.3cm3。
The preparation method of a kind of fatty tissue preparation promoting wound healing, comprises the following steps:
1) fatty tissue extraction: a certain amount of fat of aseptic aspiration is placed in centrifuge tube under abdominal part healthy skin；
2) fatty tissue processes: under aseptic technique by D-Hank ' s liquid eccentric cleaning, then cut with eye scissors
Become 0.2～0.3cm3Fritter, then add D-Hank ' s liquid clean, stand-by；
3) breviscapine dilution: utilize physiological saline solution dilution breviscapine concentration to 25mg/mL, stand-by；
4) measure the hyaluronic acid of certain volume, breviscapine and fibronectin according to the concentration of each component and join system
In standby fatty tissue granulation tissue, mix homogeneously, prepare the fatty tissue preparation promoting wound healing.
Wherein, the fatty tissue preparation promoting wound healing prepared stores under conditions of 4 DEG C.
Relative to prior art, the invention have the benefit that the fatty tissue preparation promoting wound healing of the present invention,
Use composition based on fatty tissue, and be added to hyaluronic acid, breviscapine and fibronectin, not only to fat
Tissue has bigger protective effect, and can have preferable facilitation, accelerating wound healing, the shape to cicatrix to the healing of wound surface
Become to have preferable control action, advantageously reduce the formation of cicatrix incrustation, desalination cicatrix color, improve healing quality.
Detailed description of the invention
Describe the present invention below in conjunction with specific embodiment.
Embodiment 1.
The fatty tissue preparation promoting wound healing of the present embodiment, including the component of following concentration:
The preparation method of the fatty tissue preparation promoting wound healing of the present embodiment, comprises the following steps:
1, fatty tissue extraction: aseptic aspiration 5ml fat is as 15ml centrifuge tube under abdominal part healthy skin；
2, use D-Hank ' s liquid eccentric cleaning 3 times under aseptic technique, be cut into 0.2-0.3cm with eye scissors3's
Fritter, then add D-Hank ' s liquid cleaning 2 times, stand-by；
3, with 0.9% sodium chloride injection dilution breviscapine to 25.0mg/ml, stand-by；
4, with hyaluronic acid 2.0mg/ml, the concentration of fibronectin 0.5 μ g/ml, breviscapine 2.0mg/ml has added
In the lipochondrion tissue prepared, mixing, prepared by preparation, be transferred in the syringe of 3 2ml standby.
Embodiment 2.
The fatty tissue preparation promoting wound healing of the present embodiment, including the component of following concentration:
The preparation method reference example 1 of the present embodiment, no longer repeats at this.
Embodiment 3.
The fatty tissue preparation promoting wound healing of the present embodiment, including the component of following concentration:
The preparation method reference example 1 of the present embodiment, no longer repeats at this.
Embodiment 4.
The fatty tissue preparation promoting wound healing of the present embodiment, including the component of following concentration:
The preparation method reference example 1 of the present embodiment, no longer repeats at this.
Embodiment 5.
The fatty tissue preparation promoting wound healing of the present embodiment, including the component of following concentration:
The preparation method reference example 1 of the present embodiment, no longer repeats at this.
Embodiment 6.
The fatty tissue preparation promoting wound healing of the present embodiment, including the component of following concentration:
The preparation method reference example 1 of the present embodiment, no longer repeats at this.
Compliance test result is tested: with nude mice as subjects
Choosing the healthy male and female nude mice 9 that one full year of life identical weight is about 150g, be randomly divided into three groups, one group is experiment
Group, one group be matched group, one group be blank group, tweezers are heated by alcohol burner, each nude mice skin of back of burning respectively, and wound surface is big
The little square for the length of side about 4.0cm；Experimental group nude mice uses preparation prepared by above example, and matched group uses containing only fat
The preparation of granulation tissue, blank group uses 0.9% sodium chloride injection.Under aseptic condition, disinfect the skin around wound surface, enclose
The preparation 2ml prepared more than the subcutaneous uniform point-like intramuscular injection of wound surface, completes intramuscular injection in 1h；10 days wound healings are observed in timing continuously
Situation, carries out the record of wound surface, as shown in table 1.
Nude mice wound surface area respectively organized by table 1
From upper watch test result: experimental group nude mice wound surface had begun to reduce at the 1st day, to the most extensive when the 5th day
Complete again, and do not produce cicatrix；Nude mice of control group wound surface started gray scale at the 3rd day and has recovered to the 10th day, had certain
Cicatrization；Blank group nude mice wound surface started at the 5th day to recover, wound surface size about 4.00cm2 when the 10th day, and recovering wound surface has scar
Trace is formed.
Embodiment described above only have expressed the several embodiments of the present invention, and it describes more concrete and detailed, but also
Therefore the restriction to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that, for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement, these broadly fall into the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (5)
1. promote a fatty tissue preparation for wound healing, the component including including following concentration:
Hyaluronic acid 0.05～2mg/mL
Breviscapine 0.01～2mg/mL
Fibronectin 0.01～0.5ug/mL
Surplus is fatty tissue.
The fatty tissue preparation of promotion wound healing the most according to claim 1, the consumption of described fatty tissue be 1～
5mL。
The fatty tissue preparation of promotion wound healing the most according to claim 1 and 2, the size of institute's fatty tissue granule is
0.2～0.3cm3。
4. promote a preparation method for the fatty tissue preparation of wound healing, comprise the following steps:
1) fatty tissue extraction: a certain amount of fat of aseptic aspiration is placed in centrifuge tube under abdominal part healthy skin；
2) fatty tissue processes: under aseptic technique by D-Hank ' s liquid eccentric cleaning, be then cut into 0.2 with eye scissors
～0.3cm3Fritter, then add D-Hank ' s liquid clean, stand-by；
3) breviscapine dilution: utilize physiological saline solution dilution breviscapine concentration to 25mg/mL, stand-by；
4) measure the hyaluronic acid of certain volume, breviscapine and fibronectin according to the concentration of each component and join preparation
In fatty tissue granulation tissue, mix homogeneously, prepare the fatty tissue preparation promoting wound healing.
The preparation method of fibroblast cleanout fluid the most according to claim 4, it is characterised in that prepared promotion wound
The fatty tissue preparation of healing stores under conditions of 4 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610614779.5A CN106177920A (en) | 2016-07-28 | 2016-07-28 | Adipose tissue preparation for promoting wound healing and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610614779.5A CN106177920A (en) | 2016-07-28 | 2016-07-28 | Adipose tissue preparation for promoting wound healing and preparation method thereof |
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| CN106177920A true CN106177920A (en) | 2016-12-07 |
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| CN201610614779.5A Pending CN106177920A (en) | 2016-07-28 | 2016-07-28 | Adipose tissue preparation for promoting wound healing and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114886922A (en) * | 2022-05-25 | 2022-08-12 | 温州医科大学附属第一医院 | Medicine containing fat micro-segment and application thereof in treatment of chronic wound surface difficult to heal |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101031639A (en) * | 2004-07-01 | 2007-09-05 | 马克罗珀尔生物外科公司 | Methods of using regenerative cells to promote wound healing |
| CN105311057A (en) * | 2014-07-29 | 2016-02-10 | 黄玲惠 | Cell tissue gel containing collagen and hyaluronan |
-
2016
- 2016-07-28 CN CN201610614779.5A patent/CN106177920A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101031639A (en) * | 2004-07-01 | 2007-09-05 | 马克罗珀尔生物外科公司 | Methods of using regenerative cells to promote wound healing |
| CN105311057A (en) * | 2014-07-29 | 2016-02-10 | 黄玲惠 | Cell tissue gel containing collagen and hyaluronan |
Non-Patent Citations (1)
| Title |
|---|
| 杨廷芳等: "灯盏花素液促进大鼠深Ⅱ度烧伤愈合的实验研究", 《中国医师杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114886922A (en) * | 2022-05-25 | 2022-08-12 | 温州医科大学附属第一医院 | Medicine containing fat micro-segment and application thereof in treatment of chronic wound surface difficult to heal |
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Application publication date: 20161207 |