CN104886038B - The method for making zoological specimens - Google Patents
The method for making zoological specimens Download PDFInfo
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- CN104886038B CN104886038B CN201510307690.XA CN201510307690A CN104886038B CN 104886038 B CN104886038 B CN 104886038B CN 201510307690 A CN201510307690 A CN 201510307690A CN 104886038 B CN104886038 B CN 104886038B
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Abstract
Present invention relates particularly to a kind of method for making zoological specimens.To reduce the cost of manufacture of zoological specimens, simplify making step, reduce manufacture difficulty, and reduce the injury of the material to preparation of specimen person and user such as formalin, arsenic that existing zoological specimens preparation method is used, rapid-curing cutback based on cyanoacrylate binding agent, the advantages of cohesive is strong, sealing making is carried out using cyanoacrylate binding agent, and uses it for repairing broken zoological specimens limbs, bonding animal skeleton, mimic biology circle biological phenomena.The present invention compares the making of traditional animal stripping sample, the making of animal immersed specimen with cost of manufacture, operating procedure, security and Saving specimen very big advantage, and, the field that Slide processing cannot be related to till now, i.e., the making of compound sample can be set foot in.
Description
Technical field
The present invention relates to a kind of method for making zoological specimens, and in particular to one kind cyanoacrylate binding agent system
The method for making zoological specimens.
Background technology
Sample is the firsthand information of bioscience research, is the optimal material for being engaged in practical teaching, for student,
Sample is the optimal instrument cultivated observation ability, ability of pinpointing the problems and obtain new knowledge.Existing Slide processing, especially
It is zoological specimens preparation method, anti-corrosion is carried out using noxious materials such as formalin, phenol, arsenic mostly, and these medicaments pair
Preparation of specimen person and sample user can produce a certain degree of injury, and preparation method is complicated, operating process technology will
Ask high, cost of manufacture high, this all limits the use scope and practical value of sample.
The content of the invention
Regarding to the issue above, the present invention provides a kind of method for making zoological specimens, and the method can greatly improve sample
Holding time and preservation effect, reduce preparation of specimen cost, simplify preparation of specimen the step of, eliminate preparation of specimen during
Injury using chemical reagent to preparation of specimen person and user, enables common zoological specimens fan oneself to produce animal
Sample, enriches the teaching material of teacher.
To solve the above problems, the present invention is achieved through the following technical solutions：
A kind of method for making zoological specimens of design, successively including immersion, dehydration, sealing and preservation step, using cyano group
Esters of acrylic acid binding agent is sealed.
The animal larger for cranial cavity, first with syringe to cranium intracavitary administration cyanoacrylate binding agent, consumption
About a quarter of cranial capacity, animal body surface is uniformly coated onto for the animal without fur by cyanoacrylate binding agent
Face, 2~3 millimeters of thickness can increase usage amount for the joint of insect, and by the limbs cyanoacrylate being damaged
Class binding agent is bonded.For the animal for having fur, first fur and body are peeled off, after being dehydrated completely, cyano group is used first
Esters of acrylic acid binding agent is uniformly applied to animal body surface and inner chamber, and inner cavity filled with cotton-wool, viscous with cyanoacrylate
Completely be impregnated with cotton-wool and at viscous reconciliation plane, then glued animal skin and animal body with cyanoacrylate binding agent by knot agent
With to together.
For the method for above-mentioned making zoological specimens, the soaking step is as follows：The soaking step is as follows：Take animal something lost
Body, retains its complete corpse trunk, and be pierced into a number of pin hole at its back（The effect of pin hole is：In alcohol-pickled mistake
It is more beneficial for alcohol in journey to enter in spoil, the drying of more favourable corpse when drying is using cyanoacrylate
It is more beneficial for during first binding agent seal inside binding agent immersion corpse, the quantity of pin hole is outer not influence according to animal body size
Sight is defined）, then it is immersed in 18~30 hours in alcohol, preferred volume concentration is 95% alcohol water blend；The dehydration is such as
Under：Spoil after immersion is taken out, corpse form is arranged, and fixes its form, placement dries in the sun；The preservation step
It is as follows：After spoil is dehydrated completely, it is sealed using cyanoacrylate binding agent；The preservation step is such as
Under：Spoil after sealing is dried or air-dried naturally, is preserved.
For the method for above-mentioned making zoological specimens, the soaking step is as follows：Animal skeleton is soaked in alcohol, 30
Taken out after~40 hours, change alcohol, soaked 42~50 hours again；The dehydration is as follows：Take out the animal after immersion
Bone is dried under being placed in sunlight；The sealing step is as follows：Animal skeleton arranges shape according to its physiological structure after being dehydrated
State, is sealed using cyanoacrylate binding agent animal skeleton；The preservation step is as follows：By the Animal Bone after sealing
Bone is placed under sunlight, solidifies binding agent, is preserved.
For the method for above-mentioned making zoological specimens, the cyanoacrylate binding agent is a-cyanoacrylate class
Binding agent.
For the method for above-mentioned making zoological specimens, the active ingredient of the cyanoacrylate binding agent is alpha-cyano
Ethyl acrylate.
For the method for above-mentioned making zoological specimens, the cyanoacrylate binding agent is 101 glue or 502 glue
Water, it is commercially available, also can voluntarily prepare.
The present invention has following positive beneficial effect：
Cyanoacrylate binding agent of the invention used can occur sudden reaction under the catalysis of minor amount of water in atmosphere, fast
Speed solidification and adherend is cemented, inventor by lot of experiments, using it is a kind of be totally different from conventional method by the way of make
Make zoological specimens, to make up the various defects existing for prior art.The method can greatly improve sample holding time and
The step of preservation effect, the cost of reduction preparation of specimen, simplified preparation of specimen, existing chemistry is used during elimination preparation of specimen
Injury of the reagent to preparation of specimen person and user, enables common zoological specimens fan oneself to produce zoological specimens, rich
The teaching material of Fu Liao teacher.
Specific embodiment
Specific embodiment of the invention is illustrated with reference to specific embodiment, but following examples are used only in detail
The present invention is illustrated, the scope of the present invention is limited never in any form.Involved raw material are as without especially in following embodiments
Illustrate that be commercially available, involved detection method is conventional method unless otherwise instructed.
Embodiment 1
A kind of method for making dinodon rufozonatum snake sample, comprises the following steps：
（1）Immersion：Dinodon rufozonatum snake remains are taken, retains the muscle of snake bone, skin and whole, pierced at its back using solution plane pin
Enter a number of pin hole, the size of pin hole is immersed in volumetric concentration not influence outward appearance to be preferred, then for 24 small in 95% alcohol
When；
（2）Dehydration：Dinodon rufozonatum snake corpse after immersion is taken out, corpse form is arranged, and is simultaneously dug pin with solution and fix its form, placed
Dry in the sun；
（3）Sealing：After dinodon rufozonatum snake corpse is dehydrated completely, sealed using 502 glue.
（4）Preserve：Snake after sealing is placed in into ventilation to dry, you can preserve.
The making of dinodon rufozonatum snake individuality sample saves the muscle of skin, bone and the whole of snake, while improve snake sample
Individual firmness degree, substantially increases the holding time of sample, reduces destruction of the chemical reagent to animal body.
By the comparing of different specimens preparation method it can be found that making animal mark using cyanoacrylate binding agent
No matter upper than traditional animal stripping sample, animal immersion in cost of manufacture, operating procedure, security, preservation and use sheet is
Sample etc. makes has very big advantage, is shown in Table 1.
The technology contrast table of table 1（By taking 1.5 meters of poisonless snake as an example）
101st, 502 glue belong to the glue of cyanoacrylate esters, addition polymerization can occur under the catalysis of minor amount of water in atmosphere
Reaction, solidifies rapidly and cements adherend.Because being colourless transparent liquid before its reaction, with stronger permeability, reaction
It is stable in properties after solidification, it is nontoxic, so becoming the sealing preferable reagent of zoological specimens.
Embodiment 2
A kind of compound sample（Imitate masked civet putrefaction process）Preparation method, comprise the following steps：
（1）Immersion：Masked civet bone is soaked in alcohol, is taken out after 36 hours, change alcohol, brewed 48 is small again
When；Yellow meal worm after complete death, click beetle, earwig are placed in 12 hours in alcohol；
（2）Dehydration：Masked civet bone after immersion and yellow meal worm, click beetle, earwig etc. are placed in dehydration is dried under sunlight；
Dry ground is dehydrated completely in the sun, be crushed into powder shape；
（3）Sealing：By the bone of masked civet according to its physiological structure, form is arranged in cartridge bottom, and according to sample
The form of placement arranges the thickness of dry ground；The masked civet bone for arranging and the insect for participating in rotting are entered respectively with 101 glue
Row sealing, and inlayed with insects such as yellow meal worm, click beetle, the earwigs for participating in corpse putrefaction process；
（4）Preserve：Make glue curing in the sun, and inspected periodically in following following period of time, see whether soil
Seepage, is sealed if any native seepage with 101, such as without native seepage, that is, is completed.
The making of masked civet putrefaction process sample is imitated, it is intended to certain biological phenomenon in natural imitation circle, after being made
Can be used in the middle of practical teaching process, because convenient material drawing, make simple, with very strong practicality.Illustrate to utilize cyano group
Esters of acrylic acid binding agent can not only make single individual sample, and can with natural imitation circle certain is relevant with biology
Phenomenon, set foot in the field that Slide processing cannot be related to till now, i.e., the making of compound sample.
By taking dinodon rufozonatum snake as an example, the preparation method now with skinned-specimen is as follows：
（1）Material process
Size to each position of snake body measures record, plots profile sketch, indicates body colour and common shape during life
State, the foundation as measurement data and when inserting prosthese；
（2）Peeling fleshing
Snakehead base portion is tied up into a soft rope, is hung on his thing, snakeskin is being cut about away from head veutro 3cm with scalpel
5~10cm（Otch length, depending on the size of snake body）, then, with skin and flesh at knife separating cut, and with bone shears from upper cut
Backbone is cut, a body is separated.Carefully skin is turned up again, it is drop-down on one side, while separating skin and flesh with knife.Now when carpel quilt
Tear and scale come off, and cause sample to lose shape.The intestines that be should be noted that during to anus hole and retain certain length are shelled, in case skin is torn, one
It is straight to shell to tail end.Skin is taken off from rope again, and opens the rope and short rod of binding head, then peel head skin to rhynchodaenm
Edge, rejects the muscle in remaining backbone and head, digs up eyeball and brain.If poisonous snake, cut anti-poison fang endogenous toxic material liquid and hurt sb.'s feelings, apply
Gauze encases head, slowly peels off head skin.In addition for the female snake for having ovum in abdomen, during peeling to belly, should be small by ovum hand
Heart is extruded one by one from bottom to top, and otherwise subcutaneous stripping is difficult, and causes skin to tear, and scale comes off；
（3）The making of prosthese and filling
Size according to snake body selects the iron wire of appropriate thickness, takes the iron wire for being longer than snake body 10cm length, and bamboo is wrapped with above
Silk or cotton, and fixation is bundled with fine rule, thickness length is equal with measured snake body, thin-to-thick, and change is uniform.Then fill
Enter intracutaneous.Before filling out prosthese, preservative treatment first, to smearing preservative in snakeskin, skull and spinal bone（Arsenic cream or other anti-corrosions
Medicine）, the cotton for scribbling preservative is loaded in cranial cavity, head is all to reject at muscle, loads a certain amount of cotton, and by head
Skin cylinder is turned back.
By ready-made prosthese since tail point end, the careful varus snakeskin cylinder in one side, while load prosthese up to incision,
The prosthese other end is longer than the iron wire of snake body, is fixed on vertebrae and skull, and carefully with tweezers, dissecting needle to vertebra four
Insert appropriate cotton week, make a body thickness appropriate and coincide with prosthese.Then wax wire is used（Color is consistent with snake abdomen fish）Carefully
Sew up the incision, elastic moderate, interior thing does not leak.Then the size according to eye loads onto ocular prosthesis, and according to the exact shape of snake（Body is slightly in three
It is angular）Shaping, changes its thickness uniform.
Above example is described in detail to the present invention, but, person of ordinary skill in the field it is understood that
On the premise of present inventive concept is not departed from, the design parameter of each in above-described embodiment can also be changed, be formed many
Individual specific embodiment, is common excursion of the invention, no longer describes in detail one by one herein.
Claims (5)
1. a kind of method for making zoological specimens, including immersion, dehydration, sealing and preserves step successively, it is characterised in that：Using
Cyanoacrylate binding agent is sealed；
The soaking step is as follows：Animal remains is taken, retains its complete corpse trunk, and a number of pin is pierced at its back
Hole, is then immersed in 18~30 hours in alcohol；The dehydration is as follows：Spoil after immersion is taken out, savasana is arranged
State, and its form is fixed, placement dries in the sun；The sealing step is as follows：After spoil is dehydrated completely, using cyanogen
Base esters of acrylic acid binding agent is sealed to it；The preservation step is as follows：Spoil after sealing is dried naturally or
Air-dry, preserve.
2. a kind of method for making zoological specimens, including immersion, dehydration, sealing and preserves step successively, it is characterised in that：Using
Cyanoacrylate binding agent is sealed；
The soaking step is as follows：Animal skeleton is soaked in alcohol, is taken out after 30~40 hours, change alcohol, soaked again
Bubble 42~50 hours；The dehydration is as follows：Take out under the animal skeleton after immersion is placed in sunlight and dry；The sealing step
It is as follows：Animal skeleton arranges form, using cyanoacrylate binding agent Animal Bone according to its physiological structure after being dehydrated
Bone is sealed；The preservation step is as follows：Animal skeleton after sealing is placed under sunlight, solidifies binding agent, preserved.
3. it is according to claim 1 or claim 2 make zoological specimens method, it is characterised in that the cyanoacrylate glue
Knot agent is a-cyanoacrylate class binding agent.
4. the method for making zoological specimens according to claim 3, it is characterised in that the cyanoacrylate binding agent
Active ingredient be ECA.
5. the method for making zoological specimens according to claim 3, it is characterised in that the cyanoacrylate binding agent
It is 101 glue or 502 glue.
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|---|---|---|---|
| CN201510307690.XA CN104886038B (en) | 2015-06-08 | 2015-06-08 | The method for making zoological specimens |
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| CN201510307690.XA CN104886038B (en) | 2015-06-08 | 2015-06-08 | The method for making zoological specimens |
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| CN104886038B true CN104886038B (en) | 2017-06-16 |
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| CN106257567B (en) * | 2016-03-11 | 2020-04-10 | 宁夏大学 | Earthworm section for microsurgery training, and manufacturing method and application thereof |
| CN105632313A (en) * | 2016-04-01 | 2016-06-01 | 昆山周庄鸿峰生命奥秘博物馆有限公司 | Preparation method of lizard specimen |
| CN106070183A (en) * | 2016-08-25 | 2016-11-09 | 卞陆杰 | The manufacture method of zoological specimens |
| CN106689114B (en) * | 2017-01-05 | 2021-12-07 | 卞陆杰 | Method for making unhaired vertebrate animal specimen and used injector |
| CN107183002A (en) * | 2017-05-10 | 2017-09-22 | 广西柳州中嘉知识产权服务有限公司 | Butterfly specimen preparation method |
| CN109094980B (en) * | 2018-10-09 | 2024-10-01 | 青岛农业大学 | Teflon porous rubber bottle and use method thereof |
| CN110476949B (en) * | 2019-08-22 | 2021-09-14 | 云南省热带作物科学研究所 | Preparation method of specimen of African big snail |
| JP2022124450A (en) * | 2021-10-08 | 2022-08-25 | 健次朗 酒井 | Postmortem procedure implement |
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| JP2005060361A (en) * | 2003-07-31 | 2005-03-10 | Naotada Shoji | Processing method for preserving animal and plant tissue |
| CN103430938A (en) * | 2013-08-30 | 2013-12-11 | 王荣林 | Preparation method of frog cardiovascular and saccus lymphaticus lumen colored specimen |
| CN103525119B (en) * | 2013-10-24 | 2016-01-20 | 江苏省家禽科学研究所 | A kind of cartilage staining fluid, bone stain method and utilize the method to prepare the method for embryo's skeleton specimen |
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