CN104381343B - Water-soluble compound antibacterial agent and preparation method thereof - Google Patents
Water-soluble compound antibacterial agent and preparation method thereof Download PDFInfo
- Publication number
- CN104381343B CN104381343B CN201310426524.2A CN201310426524A CN104381343B CN 104381343 B CN104381343 B CN 104381343B CN 201310426524 A CN201310426524 A CN 201310426524A CN 104381343 B CN104381343 B CN 104381343B
- Authority
- CN
- China
- Prior art keywords
- extract
- water
- folium
- extracting solution
- artemisiae argyi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a water-soluble compound antibacterial agent and its preparation method. Antibacterial active ingredients of the water-soluble compound antibacterial agent are composed of a garlic extract product, a cordate houttuynia extract product, a tarragon extract product, a chrysanthemum extract product, a folium eriobotryae extract product, a lophatherum gracile extract product and a folium cortex eucommiae extract product. Garlic, cordate houttuynia, tarragon, chrysanthemum, folium eriobotryae, lophatherum gracile and folium cortex eucommiae which have different degrees of inhibiting effects on bacteria, mould and yeast are used as raw materials to be mixed so as to prepare the antibacterial agent. Thus, antimicrobial spectrum of the antibacterial agent is broadened effectively, and the defect that current plant antibacterial agents are prepared by using a single plant as a raw material and have poor broad-spectrum antibacterial effect is overcome. The antibacterial agent can inhibit a wide range of bacteria and has better effects. The invention also discloses a preparation method of the water-soluble compound antibacterial agent.
Description
Technical field
Technical field：The present invention relates to a kind of antibacterial, more particularly, to a kind of water-soluble compound type bacteriostat, the present invention
The preparation method of the water-soluble compound type bacteriostat is further related to.
Background technology
Extending article of everyday use storage life, it is ensured that during article of everyday use quality, article of everyday use antibacterial has very important
Effect.The main component of traditional article of everyday use antibacterial is synthetic, and this synthesis antibacterial has toxicity,
Many countries are gradually limited and are used, therefore article of everyday use additive has been formed toward nontoxic, safety, what natural direction was developed
International trend.
Garlicin contained by Bulbus Allii has stronger antibacterial and anti-inflammation functions, has to various coccuses, bacillus, funguses, virus etc.
Suppress or killing action.Garlicin is to various pathogens such as staphylococcuses, meningitiss, Diplococcus pneumoniae, streptococcus and diphtheria, dysentery
Disease, escherichia coli, typhoid fever, paratyphoid fever, pertussis, tubercule bacillus and vibrio cholera etc. to be had significantly suppress or killing action.
The principle of garlicin antibacterial is due to the oxygen atom in garlicin molecule and the necessary cysteine molecule of bacterial growth breeding
In sulfydryl combine and inhibit the growth and breeding of antibacterial.Saleen et al. nineteen eighty-twos are studied and show, Bulbus Allii extract pair
The inhibitory action of the mycetes such as the antibacterials such as staphylococcus aureuses Salmonella, bacillus subtilis, escherichia coli and aspergillus oryzae, aspergillus niger
It is most strong, also there is certain inhibitory action to yeast such as beer yeast, candida mycodermas.
The main antibiotic effective ingredient decanoylacetaldehyde of Herba Houttuyniae has significantly to various bacteria, acid-fast bacilli and funguses etc.
Antibacterial action, but this kind of compound property is unstable. its sodium sulfite addition product is then stable in properties and retains its antibacterial and lives
Property. houttuynine sodium bisulfite all has obvious bacteriostasis to various Grain-positives and negative bacteria in vitro, with Staphylococcus aureus
Bacterium and its penicillin resistant strain, Diplococcus pneumoniae, alpha streptococcuss, hemophilus influenza are more sensitive, micrococcus catarrhalises, Bacillus typhi time
It, and escherichia coli, bacillus pyocyaneus and dysentery bacterium be not very sensitive. for the tubercle bacillus affection of mice, houttuynine sodium bisulfite can be substantially
Extend its life span. in addition, to Candida albicans, Cryptococcus histolyticus, sporothrix, aspergillosiss, coloring mycete, red mentagrophytes,
Trichophyton concentricum, microsporon gypseum, Microsporum ferrugineum, shark tinea bacterium etc. also have obvious inhibiting effect.
Folium Artemisiae Argyi contains volatile oil, is multicomponent mixture, and separated identification has：Terpinenol -4（Terpinenol-4）、
β-caryophyllene（β-Caryophyllene）, artemisol（Artemisia alcohol）, linalool（Linalool）, Camphora
（Camphorae）, Borneolum Syntheticum（Borneolum Syntheticum Borneol）, cineole（Cineol,Eucalyptol）And phellandrene
（Phellandrene）, Cadinene（Cadinene）, thujanol（Thujyl alcohol）Deng.
Antibacterial action
Folium Artemisiae Argyi is in vitro to anthrax bacillus, Alpha-hemolytic streptococcus, B- Streptococcus hemolyticuss, diphtheria corynebacterium, bacillus pseudodiphthericus, lung
10 kinds of Gram-positives such as diplococcoid, staphylococcus aureuses, Staphylococcus citreus, staphylococcus aureus, bacillus subtilis
Aerophil all has antibacterial action.Oleum Folium Artemisiae Argyi to Diplococcus pneumoniae, staphylococcus aureuses, Staphylococcus albus, alpha streptococcuss,
Escherichia coli, Bacillus typhi, Salmonella paratyphi, shigella flexneri etc. have bacteriostasis.With Folium Artemisiae Argyi leaf, moxa roll or Folium Artemisiae Argyi cigarette
It is smoked, can be used for indoor sterilization, mix cigarette weight with Rhizoma Atractylodis or with Rhizoma Acori Graminei and Realgar or with Rhizoma Atractylodis, Realgar, the Radix Angelicae Dahuricae etc., to golden yellow Portugal
Grape coccus, beta hemolytic streptococcuss, escherichia coli, Bacillus proteuss, diphtheria corynebacterium, typhoid fever and Salmonella paratyphi, bacillus pyocyaneus,
Bacillus subtilis, Bacillus alcaligeness and tubercule bacillus have killing or inhibiting effect.
Antifungic action
Folium Artemisiae Argyi decocting liquid is the faintest to the bacteriostasis of tinea funguses（Compared with the decocting liquid such as Rhizoma Coptidis, Radix Scutellariae）, it is dense 15%
Trichophyton when spending, starts, in suppressing, acrothesium floccosum, sufficient plantar trichophyton and Candida albicans to be removed during 30% concentration still
Development is outer, and other are oidium schoenleinii, the mutation of oidium schoenleinii Mongolia, Canis familiaris L. mountain brood cell tinea bacterium, Trichophyton concentricunm, redness
The stasi such as trichophyton, rust trichophyton, trichophyton.Cao Honglie etc. also proves the flooding agent of Folium Artemisiae Argyi（1：4）,
To trichophyton, oidium schoenleinii, Microsporon audouini, ulotrichy microsporum, red skin in test tube
The dermatophytess such as tinea bacterium, star nocardia have different degrees of inhibitory action.Meanwhile, Sun Xun determines Folium Artemisiae Argyi fumigation pair again
Oidium schoenleinii, the mutation of oidium schoenleinii Mongolia, Trichophyton concentricunm, trichophyton, trichophyton, Epidermophvton
Tinea bacterium, microsporum ferrugineum, sufficient plantar trichophyton, trichophyton interdigitale, Canis familiaris L. microsporum, trichophyton gypseum, Shen kirschner
Born of the same parents have different degrees of obvious antibacterial action in pathogenic dermatophytess such as silk bacterium, Fei Shi yeasts.
Flos Chrysanthemi has obvious bacteria resistance function, Flos Chrysanthemi water decoction or flooding agent, and in vitro tests is to various pathogens, Yi Jiliu
Influenza Virus PR8 and leptospira have certain inhibitory action, and MIC is about 1：10-1：80.Bacteriostatic test shows in mice body,
Fresh herb（Aerial partss）The volatile oil added water obtained by distillation, to staphylococcus aureuses, escherichia coli, shigella flexneri
It is stronger etc. bacteriostasis, very weak are acted on to bacillus pyocyaneus, it is invalid to Diplococcus pneumoniae.
Folium Eriobotryae has obvious bacteria resistance function, 1. contained by amygdalin can isolate hydrocyanic acid, have cough-relieving, analgesia make
With.2. amygdalin hydrolysis produces benzaldehyde and plays the role of to suppress yeast in digestive tract.3. the oils and fatss matter of Folium Eriobotryae has slightly
Phlegm-dispelling functions.4. zoopery prove Folium Eriobotryae decocting liquid have it is antibacterial, relieving asthma, phlegm-dispelling functions.5. bacteriostatic test：Folium Eriobotryae is extracted
Thing has obvious inhibitory action to Staphylococcus albus, staphylococcus aureuses, Diplococcus pneumoniae, shigella flexneri.
Herba Lophatheri has bacteria resistance function, and Herba Lophatheri is still more containing terpenoid, taraxasterol, flavonoid, organic acid, saccharide etc.
Plant active component.The heating that Herba Lophatheri causes to a variety of causes has refrigeration function；There are stronger diuresis, while can increase
The excretion of urine chloride；There is inhibitory action to staphylococcus aureuses, Streptococcus hemolyticuss.
The Cortex Eucommiae has bacteria resistance function, and Cortex Eucommiae chlorogenic acid has stronger antibacterial and anti-inflammation functions, aucubin and its polymer
There are obvious bacteriostasis, peach jaurel aglycon has inhibitory action to Gram-negative, positive bacteria.Aucubin has suppression
Bacterium, diuresis, and wound healing can be promoted；Aucubin can also produce significantly anti-together with glucoside after preculture
Virus function.
In sum, Bulbus Allii, Herba Houttuyniae, Folium Artemisiae Argyi, Flos Chrysanthemi, Folium Eriobotryae, Herba Lophatheri and Folium Eucommiae are good natural suppressions
Microbial inoculum, but to antibacterial, mycete, the inhibitory action of yeast respectively has power, but existing plant bacteriostatic agent is with single plant more
Raw material, although to a few class article of everyday use harmful bacteria inhibitions preferably, but broad-spectrum antibacterial effect is weaker, in addition in extraction process side
Face, adopts ethanol extraction process existing plant bacteriostatic agent more, and dissolubility of the ethanol extraction in water is relatively low, therefore limits
Its range of application in article of everyday use industry.
The content of the invention
An object of the present invention is to provide a kind of water-soluble compound type bacteriostat with bacteriostatic activity.
The second object of the present invention is to provide the preparation method of above-mentioned aqueous solution compound type bacteriostat.
An object of the present invention is achieved through the following technical solutions：A kind of water-soluble compound type bacteriostat, its
It is characterized in that：Its bacteriostatic active ingredients is by Bulbus Allii extract, Herba Houttuyniae extract, Folium Artemisiae Argyi extract, Flos Chrysanthemi extract, Folium Eriobotryae
Extract, Herba Lophatheri extract and Folium Eucommiae extract are constituted.
Bulbus Allii extract in described water-soluble compound type bacteriostat, Herba Houttuyniae extract, Folium Artemisiae Argyi extract, Flos Chrysanthemi is carried
The mass percent for taking thing, Folium Eriobotryae fermented product, Herba Lophatheri extract and Folium Eucommiae extract is respectively：Bulbus Allii extract 30-
40%, Herba Houttuyniae extract 10-20%, Folium Artemisiae Argyi extract 5-10%, Flos Chrysanthemi extract 10-20%, Folium Eriobotryae fermented product 5-10% are light
Folium Bambosae extract 7-10% and Folium Eucommiae extract 5-10%.
Described Bulbus Allii extract, Herba Houttuyniae extract, Folium Artemisiae Argyi extract, Flos Chrysanthemi extract, Folium Eriobotryae fermented product, phyllostachys nigra (lodd.ex lindl.) munro var.henonis (miff.) spapf et rendle
Leaf extract and Folium Eucommiae extract are respectively Bulbus Allii water extract, Herba Houttuyniae water extract, Folium Artemisiae Argyi water extract, Flos Chrysanthemi water extract, Pi
Rake leaf water extract, Herba Lophatheri water extract and Folium Eucommiae water extract.
The two of the object of the invention are achieved through the following technical solutions, a kind of above-mentioned water-soluble compound type bacteriostat
Preparation method, is characterized in that：You can well imagine with moisture and take Bulbus Allii, Herba Houttuyniae, Folium Artemisiae Argyi, Flos Chrysanthemi, Folium Eriobotryae, Herba Lophatheri and Folium Eucommiae, obtain
To Bulbus Allii water extract, Herba Houttuyniae water extract, Folium Artemisiae Argyi water extract, Flos Chrysanthemi water extract, Folium Eriobotryae water extract, Herba Lophatheri water extract and Du
Zhong Ye water extracts, mix homogeneously obtains water-soluble compound type bacteriostat.
Described Bulbus Allii water extract, Herba Houttuyniae water extract, Folium Artemisiae Argyi water extract, Flos Chrysanthemi water extract, Folium Eriobotryae water extract, phyllostachys nigra (lodd.ex lindl.) munro var.henonis (miff.) spapf et rendle
The preparation process of leaf water extract and Folium Eucommiae water extract is：
（1）By Bulbus Allii, Herba Houttuyniae, Folium Artemisiae Argyi, Flos Chrysanthemi, Folium Eriobotryae, Herba Lophatheri and Folium Eucommiae are cut into uniform thin slice after cleaning,
In 40～50 DEG C of drying, Bulbus Allii dry tablet is obtained, Herba Houttuyniae dry tablet, Folium Artemisiae Argyi dry tablet, Flos Chrysanthemi dry tablet, Folium Eriobotryae dry tablet, Herba Lophatheri does
Piece and Folium Eucommiae dry tablet；
（2）By Bulbus Allii dry tablet, Herba Houttuyniae dry tablet, Folium Artemisiae Argyi dry tablet, Flos Chrysanthemi dry tablet, Folium Eriobotryae dry tablet, Herba Lophatheri dry tablet and Du
Zhong Ye dry tablets are crushed, and garlic dry powder, Herba Houttuyniae dry powder, Folium Artemisiae Argyi dry powder, Flos Chrysanthemi dry powder, Folium Eriobotryae are obtained after 40～60 mesh sieves
Dry powder, phyllostachys nigra (lodd.ex lindl.) munro var.henonis (miff.) spapf et rendle leaf dried powder and Cortex Eucommiae leaf dried powder；
（3）Weigh garlic dry powder, Herba Houttuyniae dry powder, Folium Artemisiae Argyi dry powder, Flos Chrysanthemi dry powder, Folium Eriobotryae leaf dried powder, phyllostachys nigra (lodd.ex lindl.) munro var.henonis (miff.) spapf et rendle leaf dried powder and
Add distilled water, wherein garlic dry powder after Cortex Eucommiae leaf dried powder, Herba Houttuyniae dry powder, Folium Artemisiae Argyi dry powder, Flos Chrysanthemi dry powder, Folium Eriobotryae leaf dried powder,
Phyllostachys nigra (lodd.ex lindl.) munro var.henonis (miff.) spapf et rendle leaf dried powder and Cortex Eucommiae leaf dried powder and the weight ratio of distilled water are 1：20～30, in 50～70 DEG C of water-bath reflux, extract, 1～3
It is secondary, extract 3～5 hours every time, extracting solution is merged；
（4）Extracting solution carries out vacuum filtration, and filtrate concentrates on Rotary Evaporators, and to filtrate volume original 1/ is narrowed down to
Stop concentration when 6～1/10, obtain concentrated extracting solution；
（5）Concentrated extracting solution is vacuum dried, Bulbus Allii water extract, Herba Houttuyniae water extract, Folium Artemisiae Argyi water extract, chrysanthemum is obtained
Flower water extract, Folium Eriobotryae water extract, Herba Lophatheri water extract and Folium Eucommiae water extract.
Compared with prior art, the present invention has advantages below：
1st, with Bulbus Allii, Herba Houttuyniae, Folium Artemisiae Argyi, Flos Chrysanthemi, Folium Eriobotryae, Herba Lophatheri and Folium Eucommiae etc. to antibacterial, mycete, yeast suppression
It is raw material to make of the plant for respectively having power, has effectively expanded the antimicrobial spectrum of antibacterial, overcomes existing plant bacteriostatic agent many
With single plant as raw material, the weaker defect of broad-spectrum antibacterial effect, scope of restraining fungi is wider, and effect is more preferable.
2nd, the present invention extracts Bulbus Allii, Herba Houttuyniae, Folium Artemisiae Argyi, Flos Chrysanthemi, Folium Eriobotryae, Herba Lophatheri and Folium Eucommiae, system using water extraction
Into antibacterial water solublity it is very good, overcome existing plant bacteriostatic agent to dissolve in water using the extract obtained by alcohol extraction process
The relatively low defect of degree, has expanded its range of application in article of everyday use industry, i.e., as a kind of safe and efficient natural anticorrosion
Agent, can be widely applied to all kinds of article of everyday use industries.
3rd, from Bulbus Allii dry tablet, Herba Houttuyniae dry tablet, Folium Artemisiae Argyi dry tablet, Flos Chrysanthemi dry tablet, Folium Eriobotryae dry tablet, Herba Lophatheri dry tablet and the Cortex Eucommiae
Water extract is extracted in leaf and prepares antibacterial, can be with the receipts of Bulbus Allii, Herba Houttuyniae, Folium Artemisiae Argyi, Flos Chrysanthemi, Folium Eriobotryae, Herba Lophatheri and Folium Eucommiae
Obtain season and purchased in a large number with relatively low expense, it is standby to make dry tablet, can not only produce throughout the year, can also reduce product cost.
Specific embodiment
With reference to embodiments the present invention is illustrated with comparative example, and protection scope of the present invention is not only limited to
In following examples.The those of ordinary skill of the technical field is capable of achieving the present invention's according to present disclosure
Purpose.
Embodiment one
The following steps are prepared into water-soluble compound type bacteriostat：
The preparation of 1 Bulbus Allii extract
1.1 using commercially available fresh garlic as raw material, to clean and be cut into uniform thin slice with microtome after peeling, in 45 DEG C
Drying, obtains Bulbus Allii dry tablet；
1.2 crush the Bulbus Allii dry tablet obtained in step 1.1, and after 60 mesh sieves garlic dry powder is obtained；
1.3 weigh the garlic dry powder obtained in a certain amount of step 1.2, with garlic dry powder：Water=1：25 weight is than adding
Distilled water, in 60 DEG C of water-bath reflux, extract, 3 times, 3 hours every time, the said extracted extracting solution of 3 times is merged；
The extracting solution obtained in step 1.3 is carried out vacuum filtration by 1.4, and filtrate concentrates on Rotary Evaporators, works as filtrate
Volume-diminished obtains concentrated extracting solution to concentration is stopped when original 1/8；
1.5 are spray-dried the concentrated extracting solution obtained in step 1.4, obtain Bulbus Allii water extract.
The preparation of 2 Herba Houttuyniae extracts
2.1 using commercially available fresh Herba Houttuyniae as raw material, to clean and be cut into uniform thin slice with microtome after peeling, in 45
DEG C drying, obtain Herba Houttuyniae dry tablet；
2.2 crush the Herba Houttuyniae dry tablet obtained in step 2.1, and Herba Houttuyniae dry powder is obtained after 60 mesh sieves；
2.3 weigh the Herba Houttuyniae dry powder obtained in a certain amount of step 2.2, with Herba Houttuyniae dry powder：Water=1：25 weight ratio
Distilled water is added, in 60 DEG C of water-bath reflux, extract, 3 times, 3 hours every time, the said extracted extracting solution of 3 times is merged；
The extracting solution obtained in step 2.3 is carried out vacuum filtration by 2.4, and filtrate concentrates on Rotary Evaporators, works as filtrate
Volume-diminished obtains concentrated extracting solution to concentration is stopped when original 1/8；
2.5 are spray-dried the concentrated extracting solution obtained in step 2.4, obtain Herba Houttuyniae water extract.
The preparation of 3 Folium Artemisiae Argyi extracts
3.1 using commercially available fresh Folium Artemisiae Argyi as raw material, to clean and be cut into uniform thin slice with microtome after peeling, in 45 DEG C
Drying, obtains Folium Artemisiae Argyi dry tablet；
3.2 crush the Folium Artemisiae Argyi dry tablet obtained in step 3.1, and Folium Artemisiae Argyi dry powder is obtained after 60 mesh sieves；
3.3 weigh the Folium Artemisiae Argyi dry powder obtained in a certain amount of step 3.2, with Folium Artemisiae Argyi dry powder：Water=1：25 weight is than adding
Distilled water, in 60 DEG C of water-bath reflux, extract, 3 times, 3 hours every time, the said extracted extracting solution of 3 times is merged；
The extracting solution obtained in step 3.3 is carried out vacuum filtration by 3.4, and filtrate concentrates on Rotary Evaporators, works as filtrate
Volume-diminished obtains concentrated extracting solution to concentration is stopped when original 1/8；
3.5 are spray-dried the concentrated extracting solution obtained in step 3.4, obtain Folium Artemisiae Argyi water extract.
The preparation of 4 Flos Chrysanthemi extracts
4.1 using commercially available fresh chrysanthemum as raw material, to clean and be cut into uniform thin slice with microtome after peeling, in 45 DEG C
Drying, obtains Flos Chrysanthemi dry tablet；
4.2 crush the Flos Chrysanthemi dry tablet obtained in step 4.1, and Flos Chrysanthemi dry powder is obtained after 60 mesh sieves；
4.3 weigh the Flos Chrysanthemi dry powder obtained in a certain amount of step 4.2, with Flos Chrysanthemi dry powder：Water=1：25 weight is than adding
Distilled water, in 60 DEG C of water-bath reflux, extract, 3 times, 3 hours every time, the said extracted extracting solution of 3 times is merged；
The extracting solution obtained in step 4.3 is carried out vacuum filtration by 4.4, and filtrate concentrates on Rotary Evaporators, works as filtrate
Volume-diminished obtains concentrated extracting solution to concentration is stopped when original 1/8；
4.5 are spray-dried the concentrated extracting solution obtained in step 4.4, obtain Flos Chrysanthemi water extract.
The preparation of 5 Folium Eriobotryae fermented product
5.1 using commercially available fresh Folium Eriobotryae as raw material, to clean and be cut into uniform thin slice with microtome after peeling, in 45
DEG C drying, obtain Folium Eriobotryae dry tablet；
5.2 crush the Folium Eriobotryae dry tablet obtained in step 5.1, and Folium Eriobotryae leaf dried powder is obtained after 60 mesh sieves；
5.3 weigh the Folium Eriobotryae leaf dried powder obtained in a certain amount of step 5.2, with Folium Eriobotryae leaf dried powder：Water=1：25 weight ratio
Distilled water is added, in 60 DEG C of water-bath reflux, extract, 3 times, 3 hours every time, the said extracted extracting solution of 3 times is merged；
The extracting solution obtained in step 5.3 is carried out vacuum filtration by 5.4, and filtrate concentrates on Rotary Evaporators, works as filtrate
Volume-diminished obtains concentrated extracting solution to concentration is stopped when original 1/8；
5.5 are spray-dried the concentrated extracting solution obtained in step 5.4, obtain Folium Eriobotryae water extract.
The preparation of 6 Herba Lophatheri extracts
6.1 using commercially available fresh Herba Lophatheri as raw material, to clean and be cut into uniform thin slice with microtome after peeling, in 45
DEG C drying, obtain Herba Lophatheri dry tablet；
6.2 crush the Herba Lophatheri dry tablet obtained in step 6.1, and phyllostachys nigra (lodd.ex lindl.) munro var.henonis (miff.) spapf et rendle leaf dried powder is obtained after 60 mesh sieves；
6.3 weigh the phyllostachys nigra (lodd.ex lindl.) munro var.henonis (miff.) spapf et rendle leaf dried powder obtained in a certain amount of step 6.2, with phyllostachys nigra (lodd.ex lindl.) munro var.henonis (miff.) spapf et rendle leaf dried powder：Water=1：25 weight ratio
Distilled water is added, in 60 DEG C of water-bath reflux, extract, 3 times, 3 hours every time, the said extracted extracting solution of 3 times is merged；
The extracting solution obtained in step 6.3 is carried out vacuum filtration by 6.4, and filtrate concentrates on Rotary Evaporators, works as filtrate
Volume-diminished obtains concentrated extracting solution to concentration is stopped when original 1/8；
6.5 are spray-dried the concentrated extracting solution obtained in step 6.4, obtain Herba Lophatheri water extract.
The preparation of 7 Folium Eucommiae extracts
7.1 using commercially available fresh Folium Eucommiae as raw material, to clean and be cut into uniform thin slice with microtome after peeling, in 45
DEG C drying, obtain Folium Eucommiae dry tablet；
7.2 crush the Folium Eucommiae obtained in step 7.1, and Cortex Eucommiae leaf dried powder is obtained after 60 mesh sieves；
7.3 weigh the Cortex Eucommiae leaf dried powder obtained in a certain amount of step 7.2, with Cortex Eucommiae leaf dried powder：Water=1：25 weight ratio
Distilled water is added, in 60 DEG C of water-bath reflux, extract, 3 times, 3 hours every time, the said extracted extracting solution of 3 times is merged；
The extracting solution obtained in step 7.3 is carried out vacuum filtration by 7.4, and filtrate concentrates on Rotary Evaporators, works as filtrate
Volume-diminished obtains concentrated extracting solution to concentration is stopped when original 1/8；
7.5 are spray-dried the concentrated extracting solution obtained in step 7.4, obtain Folium Eucommiae water extract.
4. the preparation of antibacterial
Above-mentioned Bulbus Allii extract 34%, Herba Houttuyniae extract 17%, Folium Artemisiae Argyi extract are weighed by the percentage by weight of each component
7%, Flos Chrysanthemi extract 17%, Folium Eriobotryae fermented product 8%, Herba Lophatheri extract 9% and Folium Eucommiae extract 8%.Compounded, mixed
Water-soluble compound type bacteriostat sample is obtained after uniform.
Embodiment two
The preparation of antibacterial
Above-mentioned Bulbus Allii extract 35%, Herba Houttuyniae extract 15%, Folium Artemisiae Argyi extract are weighed by the percentage by weight of each component
8%, Flos Chrysanthemi extract 16%, Folium Eriobotryae fermented product 8%, Herba Lophatheri extract 9% and Folium Eucommiae extract 9%.Compounded, mixed
Water-soluble compound type bacteriostat sample is obtained after uniform.
Embodiment three
The preparation of antibacterial
Above-mentioned Bulbus Allii extract 36%, Herba Houttuyniae extract 16%, Folium Artemisiae Argyi extract are weighed by the percentage by weight of each component
8%, Flos Chrysanthemi extract 16%, Folium Eriobotryae fermented product 8%, Herba Lophatheri extract 9% and Folium Eucommiae extract 7%.Compounded, mixed
Water-soluble compound type bacteriostat sample is obtained after uniform.
Experimental example：
Take the water-soluble compound type bacteriostat obtained by above-described embodiment one to three, Flos Chrysanthemi water extract, Herba Houttuyniae water extract,
The sample of Bulbus Allii water extract carries out antibacterial contrast experiment, and the bacteriostatic activity evaluation for drawing is shown in Table 1
Strain
(1), antibacterial：Staphylococcus aureuses, Salmonella, bacillus subtilis, escherichia coli
(2), funguses：Aspergillus niger, aspergillus oryzae, Aspergillus citrimum, beer yeast, rhodotorula glutinis, candida mycoderma
Culture medium
（One）Inoculum：LB
Such as to configure solid LB media, plus 15.0g agaroses/L
(2) fungus culture medium：Potato culture (PDA)
Equipment and galenical
Culture dish（90mm）, filter paper, triangle rake, inoculating loop, test tube
Galenical：Bulbus Allii extract, Herba Houttuyniae extract, Flos Chrysanthemi extract, Folium Artemisiae Argyi extract, Folium Eriobotryae fermented product,
Herba Lophatheri extract, Folium Eucommiae extract, compound botanical extract
Experimental procedure
Minimal inhibitory concentration determines (MIC)-test tube method
Test tube method test is done to each tested strain.
Culture medium is sub-packed in test tube (18mm × 180mm) with every pipe final volume 5m1, is gone out under the conditions of 120 DEG C, 0.1MPa
Bacterium 20min, when sterilising medium is cooled to 50 DEG C or so, sterile working adds sample fully to mix, and doubling dilution is into (volume
Than) series concentration, sterilized water compares, and is respectively prepared inclined-plane.Well-grown tested bacterium streak inoculation is taken on inclined-plane.Antibacterial
In being put in 30 DEG C of incubators, bacteria growing state is observed in 24h, funguses are put in 27 DEG C of incubators, result is observed after 72h, weight is set
Multiple group.Estimate and with following standard recording：Bacterium is entirely without growth " "；Bacteria growing is slow, the bacterium colony very little "+" of formation：Bacterium gives birth to
It is long more larger " ++ " than very fast formation bacterium colony；Bacteria growing is rapid but bacterium colony is than normal little " +++ "；Bacteria growing rapidly with it is normal it is identical "+
++ ten ".It is minimum inhibitory concentration (MIC) to take the complete not minimum sample concentration of growth tube.Table 1：Flos Chrysanthemi extract, Herba Houttuyniae is extracted
The minimum inhibitory concentration of thing, Bulbus Allii extract and water-soluble compound type bacteriostat
From table 1, water extract for examination bacterium to having different degrees of inhibitory action.Wherein, Flos Chrysanthemi water extract is to golden yellow
The inhibitory action of the antibacterials such as color staphylococcuses, Salmonella, bacillus subtilis, escherichia coli is most strong, is secondly Bulbus Allii water extract, then
Secondary Herba Houttuyniae water extract is most strong to the inhibitory action of the mycetes such as aspergillus oryzae, aspergillus niger and Aspergillus citrimum for Herba Houttuyniae water extract, secondly
It is Bulbus Allii extract, is again Flos Chrysanthemi water extract, Herba Houttuyniae water extract is to yeast such as beer yeast, rhodotorula glutinis and candida mycodermas
Inhibitory action it is most strong, next to that Flos Chrysanthemi water extract, is again Bulbus Allii water extract.
As can also be seen from Table 1, the antibacterial for preparing is compounded with above-mentioned water extract, to staphylococcus aureuses, aspergillus niger with
And the inhibitory action of aspergillus oryzae and the inhibitory action of other microorganisms are equal to or are better than the most strong single plant of inhibitory action
Thing extract, illustrates the fungistatic effect of plant bacteriostatic agent that Jing compoundings obtain more preferably, and antimicrobial spectrum is bigger, with wide spectrum, efficiently
Bacteriostasis.
Claims (8)
1. a kind of water-soluble compound type bacteriostat, is characterized in that：It is mainly by Bulbus Allii extract, Herba Houttuyniae extract, Folium Artemisiae Argyi
Extract, Flos Chrysanthemi extract, Folium Eriobotryae fermented product, Herba Lophatheri extract and Folium Eucommiae extract are constituted；Described Bulbus Allii is extracted
Thing, Herba Houttuyniae extract, Folium Artemisiae Argyi extract, Flos Chrysanthemi extract, Folium Eriobotryae fermented product, Herba Lophatheri extract and Folium Eucommiae extract
Mass percent be respectively：Bulbus Allii extract 30-40%, Herba Houttuyniae extract 10-20%, Folium Artemisiae Argyi extract 5-10%, chrysanthemum
Flower extract 10-20%, Folium Eriobotryae fermented product 5-10%, Herba Lophatheri extract 7-10% and Folium Eucommiae extract 5-10%；
Described Bulbus Allii extract, Herba Houttuyniae extract, Folium Artemisiae Argyi extract, Flos Chrysanthemi extract, Folium Eriobotryae fermented product, Herba Lophatheri carries
Take thing and Folium Eucommiae extract is respectively Bulbus Allii water extract, Herba Houttuyniae water extract, Folium Artemisiae Argyi water extract, Flos Chrysanthemi water extract, Folium Eriobotryae
Water extract, Herba Lophatheri water extract and Folium Eucommiae water extract.
2. a kind of method of the water-soluble compound type bacteriostat prepared described in claim 1, is characterized in that：You can well imagine with moisture and take greatly
Bulbus Allii, Herba Houttuyniae, Folium Artemisiae Argyi, Flos Chrysanthemi, Folium Eriobotryae, Herba Lophatheri and Folium Eucommiae, obtain Bulbus Allii water extract, Herba Houttuyniae water extract, Folium Artemisiae Argyi water
Extract, Flos Chrysanthemi water extract, Folium Eriobotryae water extract, Herba Lophatheri water extract and Folium Eucommiae water extract, mix homogeneously obtains water solublity
Compound type bacteriostat.
3. the preparation method of water-soluble compound type bacteriostat according to claim 2：It is characterized in that：Described Bulbus Allii is extracted
The preparation process of thing：
A, after Bulbus Allii is cleaned uniform thin slice is cut into, and in the drying of 40-50 degree, obtains Bulbus Allii dry tablet；
B crushes Bulbus Allii dry tablet, and after 40-60 mesh sieves garlic dry powder is obtained；
C is weighed and add after garlic dry powder distilled water, and wherein garlic dry powder and the weight ratio of distilled water are 1:20-1:30, in 50-70
Degree water-bath reflux, extract, 1-3 time, extracts every time 3-5 hours, and extracting solution is merged.
D extracting solution carries out vacuum filtration, and filtrate concentrates on Rotary Evaporators, and to filtrate volume original 1/6-1/10 is narrowed down to
When stop concentration, obtain concentrated solution extracting solution；
E is vacuum dried concentrated extracting solution, obtains Bulbus Allii water extract.
4. the preparation method of water-soluble compound type bacteriostat according to claim 2：It is characterized in that：Described Herba Houttuyniae is carried
Take the preparation process of thing：
A, after Herba Houttuyniae is cleaned uniform thin slice is cut into, and in the drying of 40-50 degree, obtains Herba Houttuyniae dry tablet；
B crushes Herba Houttuyniae dry tablet, and Herba Houttuyniae dry powder is obtained after 40-60 mesh sieves；
C is weighed and distilled water is added after Herba Houttuyniae dry powder, and wherein Herba Houttuyniae dry powder and the weight ratio of distilled water are 1:20-1:30, in
50-70 degree water-baths reflux, extract, 1-3 time, extracts every time 3-5 hours, and extracting solution is merged；
D extracting solution carries out vacuum filtration, and filtrate concentrates on Rotary Evaporators, and to filtrate volume original 1/6-1/10 is narrowed down to
When stop concentration, obtain concentrated solution extracting solution；
E is vacuum dried concentrated extracting solution, obtains Herba Houttuyniae water extract.
5. the preparation method of water-soluble compound type bacteriostat according to claim 2：It is characterized in that：Described Folium Artemisiae Argyi is extracted
The preparation process of thing：
A, after Folium Artemisiae Argyi is cleaned uniform thin slice is cut into, and in the drying of 40-50 degree, obtains Folium Artemisiae Argyi dry tablet；
B crushes Folium Artemisiae Argyi dry tablet, and Folium Artemisiae Argyi dry powder is obtained after 40-60 mesh sieves；
C is weighed and distilled water is added after Folium Artemisiae Argyi dry powder, and wherein Folium Artemisiae Argyi dry powder and the weight ratio of distilled water are 1:20-1:30, in 50-70
Degree water-bath reflux, extract, 1-3 time, extracts every time 3-5 hours, and extracting solution is merged；
D extracting solution carries out vacuum filtration, and filtrate concentrates on Rotary Evaporators, and to filtrate volume original 1/6-1/10 is narrowed down to
When stop concentration, obtain concentrated solution extracting solution；
E is vacuum dried concentrated extracting solution, obtains Folium Artemisiae Argyi water extract.
6. the preparation method of water-soluble compound type bacteriostat according to claim 2：It is characterized in that：Described Flos Chrysanthemi is extracted
The preparation process of thing：
A, after Flos Chrysanthemi is cleaned uniform thin slice is cut into, and in the drying of 40-50 degree, obtains Flos Chrysanthemi dry tablet；
B crushes Flos Chrysanthemi dry tablet, and Flos Chrysanthemi dry powder is obtained after 40-60 mesh sieves；
C is weighed and distilled water is added after Flos Chrysanthemi dry powder, and wherein Flos Chrysanthemi dry powder and the weight ratio of distilled water are 1:20-1:30, in 50-70
Degree water-bath reflux, extract, 1-3 time, extracts every time 3-5 hours, and extracting solution is merged；
D extracting solution carries out vacuum filtration, and filtrate concentrates on Rotary Evaporators, and to filtrate volume original 1/6-1/10 is narrowed down to
When stop concentration, obtain concentrated solution extracting solution；
E is vacuum dried concentrated extracting solution, obtains Flos Chrysanthemi water extract.
7. the preparation method of water-soluble compound type bacteriostat according to claim 2：It is characterized in that：Described Folium Eriobotryae is carried
Take the preparation process of thing：
A is cut into uniform thin slice after Folium Eriobotryae is cleaned, and in the drying of 40-50 degree, obtains Folium Eriobotryae dry tablet；
B crushes Folium Eriobotryae dry tablet, and Folium Eriobotryae leaf dried powder is obtained after 40-60 mesh sieves；
C is weighed and distilled water is added after Folium Eriobotryae leaf dried powder, and wherein Folium Eriobotryae leaf dried powder and the weight ratio of distilled water are 1:20-1:30, in
50-70 degree water-baths reflux, extract, 1-3 time, extracts every time 3-5 hours, and extracting solution is merged；
D extracting solution carries out vacuum filtration, and filtrate concentrates on Rotary Evaporators, and to filtrate volume original 1/6-1/10 is narrowed down to
When stop concentration, obtain concentrated solution extracting solution；
E is vacuum dried concentrated extracting solution, obtains Folium Eriobotryae water extract.
8. the preparation method of water-soluble compound type bacteriostat according to claim 2：It is characterized in that：Described Herba Lophatheri or
The preparation process of Folium Eucommiae leaf extract：
A is cut into uniform thin slice after cleaning Herba Lophatheri or Folium Eucommiae, in the drying of 40-50 degree, obtains Herba Lophatheri or Folium Eucommiae；
B crushes Herba Lophatheri or Folium Eucommiae, and Herba Lophatheri or Cortex Eucommiae leaf dried powder are obtained after 40-60 mesh sieves；
C is weighed and distilled water is added after Herba Lophatheri or Cortex Eucommiae leaf dried powder, wherein the weight of Herba Lophatheri or Cortex Eucommiae leaf dried powder and distilled water
Than for 1:20-1:30, in 50-70 degree water-baths reflux, extract, 1-3 time, 3-5 hours are extracted every time, extracting solution is merged；
D extracting solution carries out vacuum filtration, and filtrate concentrates on Rotary Evaporators, and to filtrate volume original 1/6-1/10 is narrowed down to
When stop concentration, obtain concentrated solution extracting solution；
E is vacuum dried concentrated extracting solution, obtains Herba Lophatheri or Folium Eucommiae water extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310426524.2A CN104381343B (en) | 2013-09-17 | 2013-09-17 | Water-soluble compound antibacterial agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310426524.2A CN104381343B (en) | 2013-09-17 | 2013-09-17 | Water-soluble compound antibacterial agent and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104381343A CN104381343A (en) | 2015-03-04 |
| CN104381343B true CN104381343B (en) | 2017-05-10 |
Family
ID=52599393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310426524.2A Expired - Fee Related CN104381343B (en) | 2013-09-17 | 2013-09-17 | Water-soluble compound antibacterial agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104381343B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104738110A (en) * | 2015-04-03 | 2015-07-01 | 民勤县腾盛种业有限公司 | Botanical insecticide for preventing and treating zucchini pythium rot |
| CN105104394B (en) * | 2015-09-24 | 2017-12-26 | 山东汤普乐作物科学有限公司 | The trans application to coumaric acyl tormentic acid of compound 23 |
| CN105557776A (en) * | 2015-12-04 | 2016-05-11 | 广德县先雨农业有限公司 | Adinandra milletii and illicium anisatum stem rot prevention and treatment agent and preparation method thereof |
| CN105558750A (en) * | 2015-12-22 | 2016-05-11 | 贵州大方阳光佳源生态食品有限公司 | Chinese herb preservative for salted products |
| CN106804634A (en) * | 2016-11-29 | 2017-06-09 | 朱朝阳 | A kind of biological agent and its preparation technology for preventing and treating early blight of tomato |
| CN107998048A (en) * | 2017-12-25 | 2018-05-08 | 芜湖皖江知识产权运营中心有限公司 | A kind of bacteriostatic agent and preparation method thereof |
| CN108419828A (en) * | 2018-03-21 | 2018-08-21 | 江西农业大学 | A kind of citrus comprising loquat-leaf extract adopts rear plant antistaling agent |
| CN109258788A (en) * | 2018-08-09 | 2019-01-25 | 兴义市天瑞核桃种植农民专业合作社 | A kind of preservation method of green rind walnut |
| CN110024812B (en) * | 2019-04-10 | 2021-03-16 | 湖北中医药大学 | Natural weed inhibitor taking moxa powder as raw material and preparation method thereof |
| CN110959773B (en) * | 2019-12-02 | 2023-03-24 | 广西壮族自治区水产科学研究院 | Feed additive and feed for preventing streptococcicosis of tilapia mossambica, and preparation method and application of feed additive and feed |
| CN113598202B (en) * | 2021-08-11 | 2022-06-24 | 广东开放大学（广东理工职业学院） | Antibacterial composition and application thereof in preparation of biodegradable plastic |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1108475A (en) * | 1995-01-14 | 1995-09-20 | 阎歧斌 | Sterilizing incense |
| CN1740120A (en) * | 2004-08-25 | 2006-03-01 | 杭州山缘投资有限公司 | Lead reducing pest resisting selenium-rich top dressing specially for organic tea tree |
| CN1799364A (en) * | 2004-12-31 | 2006-07-12 | 风间行男 | Preparation method of insects repellant and insects repelling method |
| CN101647474A (en) * | 2009-09-17 | 2010-02-17 | 中国计量学院 | Plant-derived bacteriostatic extractive and application thereof |
| CN102428852A (en) * | 2011-09-01 | 2012-05-02 | 中国农业科学院麻类研究所 | Degradable agricultural natural insect-repelling mulching film and preparation method thereof |
| CN102715197A (en) * | 2012-06-21 | 2012-10-10 | 袁静 | Aerosol disinfectant |
-
2013
- 2013-09-17 CN CN201310426524.2A patent/CN104381343B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1108475A (en) * | 1995-01-14 | 1995-09-20 | 阎歧斌 | Sterilizing incense |
| CN1740120A (en) * | 2004-08-25 | 2006-03-01 | 杭州山缘投资有限公司 | Lead reducing pest resisting selenium-rich top dressing specially for organic tea tree |
| CN1799364A (en) * | 2004-12-31 | 2006-07-12 | 风间行男 | Preparation method of insects repellant and insects repelling method |
| CN101647474A (en) * | 2009-09-17 | 2010-02-17 | 中国计量学院 | Plant-derived bacteriostatic extractive and application thereof |
| CN102428852A (en) * | 2011-09-01 | 2012-05-02 | 中国农业科学院麻类研究所 | Degradable agricultural natural insect-repelling mulching film and preparation method thereof |
| CN102715197A (en) * | 2012-06-21 | 2012-10-10 | 袁静 | Aerosol disinfectant |
Non-Patent Citations (3)
| Title |
|---|
| 《杜仲叶提取物的抑菌活性研究》;季志平，苏印泉;《林产化学与工业》;20080229;第28卷(第1期);第63-66页 * |
| 《枇杷叶提取物抑菌作用研究》;肖新生，林倩英;《现代食品科技》;20101231;第26卷(第1期);第59-62页 * |
| 《艾叶水溶性部位及其分离组分的抑菌活性研究》;尹美珍，张倩，王静晖，等;《黄石理工学院学报》;20111031;第27卷(第5期);第44-48页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104381343A (en) | 2015-03-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104381343B (en) | Water-soluble compound antibacterial agent and preparation method thereof | |
| CN104255823B (en) | A kind of plant bacteriostatic composition and its application in liquid detergent | |
| CN103451032B (en) | Safe and environment-friendly tea seed washing powder and preparation method thereof | |
| CN113244296B (en) | Medicinal volatile oil composition and preparation method and application thereof | |
| CN104094977A (en) | Composition with insect-expelling sterilization disinfection effects and application thereof | |
| CN104938544A (en) | Method for preparing natural preservative and application thereof | |
| CN111419947A (en) | Folium artemisiae argyi compound essential oil capable of relieving throat pain and preparation method thereof | |
| CN106109351B (en) | Sterilization repair cleaning agent, preparation method and application thereof | |
| CN103990165A (en) | Multifunctional anti-influenza liquid aromatizer and preparation method | |
| CN101422419B (en) | Toilet water and manufacture method thereof | |
| CN103720005A (en) | Preparation method of all-natural plant food preservative | |
| CN101181208A (en) | Gargle containing natural antibiotics and preparation method thereof | |
| KR101795917B1 (en) | Antimicrobial compositions for perfume containing natural material extract | |
| CN101449804A (en) | Extraction method of Mongolian snakegourd effective ingredient | |
| WO2018099493A1 (en) | Tropaeolum majus granules and preparation method therefor | |
| CN103263679A (en) | Drug-smelling plant air clean fluid for purifying environment and reinforcing respiratory system | |
| CN103815587B (en) | A kind ofly harmful fine particulates is effectively stoped in air to suck and mouth mask with bacteriostasis and preparation method thereof | |
| CN113633685B (en) | Traditional Chinese medicine composition with antibacterial activity and application | |
| CN104491121A (en) | Anti-bacterial essence solution for treating respiratory diseases and preparation method of anti-bacterial essence solution | |
| KR102252009B1 (en) | Antiviral or antibacterial composition containing extracts of Torreya nucifera leaf | |
| ONUEGBU et al. | Phytochemical and In Vitro Antimicrobial Properties of Premna hispida (Verbenaceae) Linn. | |
| CN107540532A (en) | A kind of diphenyl ether compound with antibacterial activity in honeysuckle and preparation method and application | |
| CN115104690A (en) | Natural food preservative and preparation method thereof | |
| CN113081917A (en) | Female care solution containing hemp leaf extract and preparation method thereof | |
| CN112006042A (en) | Traditional Chinese medicine composition for disinfection, traditional Chinese medicine preparation, preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170510 Termination date: 20200917 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |