CA2206848A1 - Production de peptides au moyen de proteines de fusion de recombinaison - Google Patents
Production de peptides au moyen de proteines de fusion de recombinaisonInfo
- Publication number
- CA2206848A1 CA2206848A1 CA 2206848 CA2206848A CA2206848A1 CA 2206848 A1 CA2206848 A1 CA 2206848A1 CA 2206848 CA2206848 CA 2206848 CA 2206848 A CA2206848 A CA 2206848A CA 2206848 A1 CA2206848 A1 CA 2206848A1
- Authority
- CA
- Canada
- Prior art keywords
- fusion protein
- protein construct
- solution
- fragment
- carbonic anhydrase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 238000013519 translation Methods 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
- 108010080629 tryptophan-leucine Proteins 0.000 description 1
- 229960004441 tyrosine Drugs 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 229960003726 vasopressin Drugs 0.000 description 1
- 229940006486 zinc cation Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention décrit un procédé pour isoler et/ou purifier un peptide de recombinaison à l'aide d'une protéine de fusion de synthèse incluant une anhydrase carbonique et un polypeptide fusionné variable. Ce procédé comporte la précipitation, soit de la protéine de fusion de synthèse, soit d'un fragment de celle-ci incluant l'anhydrase carbonique. L'invention décrit aussi des corps d'inclusion qui incluent la protéine de fision de synthèse et un procédé de production d'un peptide incluant l'expression de la protéine de fusion de synthèse comme faisant partie d'un corps d'inclusion. Les protéines de fusion de synthèse qui incluent une anhydrase carbonique et certains peptides cibles sont également décrits.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35053094A | 1994-12-07 | 1994-12-07 | |
| US08/350,530 | 1994-12-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2206848A1 true CA2206848A1 (fr) | 1996-06-13 |
Family
ID=23377117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2206848 Abandoned CA2206848A1 (fr) | 1994-12-07 | 1995-12-07 | Production de peptides au moyen de proteines de fusion de recombinaison |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0796335A1 (fr) |
| JP (1) | JPH10510155A (fr) |
| AU (1) | AU700605B2 (fr) |
| CA (1) | CA2206848A1 (fr) |
| WO (1) | WO1996017942A1 (fr) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2196536A1 (fr) | 1997-01-08 | 2010-06-16 | Life Technologies Corporation | Méthodes pour la production des protéines |
| US7488590B2 (en) | 1998-10-23 | 2009-02-10 | Amgen Inc. | Modified peptides as therapeutic agents |
| TWI257394B (en) | 1998-10-23 | 2006-07-01 | Kirin Amgen Inc | Thrombopoietic compounds |
| US6660843B1 (en) | 1998-10-23 | 2003-12-09 | Amgen Inc. | Modified peptides as therapeutic agents |
| WO2000037615A1 (fr) * | 1998-12-21 | 2000-06-29 | Eli Lilly And Company | Synthese par recombinaison de beta-lipotropine et d'autres peptides |
| DK1463751T3 (da) | 2001-12-21 | 2013-08-26 | Human Genome Sciences Inc | Albuminfusionsproteiner. |
| AU2003212348A1 (en) * | 2002-03-12 | 2003-09-22 | Dsm Ip Assets B.V. | Hepatitis c virus replicon containing a reporter gene and a selectable marker gene |
| WO2004022004A2 (fr) * | 2002-09-06 | 2004-03-18 | Bayer Pharmaceuticals Corporation | Agonistes modifies du recepteur glp-1 et leurs procedes pharmacologiques d'utilisation |
| TW200643033A (en) * | 2005-03-08 | 2006-12-16 | Chugai Pharmaceutical Co Ltd | Conjugate of water-soluble modified hyaluronic acid and glp-1 analogue |
| US8008453B2 (en) | 2005-08-12 | 2011-08-30 | Amgen Inc. | Modified Fc molecules |
| US20090087873A1 (en) | 2006-07-21 | 2009-04-02 | Invitrogen Corporation | Sharply resolving labeled protein molecular weight standards |
| US20100273983A1 (en) * | 2007-04-05 | 2010-10-28 | The University Of Queensland | Method of purifying peptides by selective precipitation |
| CL2016003427A1 (es) | 2016-12-30 | 2017-06-23 | Univ Chile | Una secuencia peptídica de una proteína guía para la producción de un péptido de interés; un vector de expresión; una célula huésped; un proceso para para la producción de un péptido de interés. |
| CN113340691B (zh) * | 2021-06-05 | 2023-12-12 | 清华大学 | 一种提取海洋沉积物中不同赋存形态磷的方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2535066B2 (ja) * | 1987-03-20 | 1996-09-18 | クリエイティブ・バイオマリキュールズ・インコーポレーテッド | 組換え体タンパク質製造のためのリ―ダ―配列 |
| ATE113985T1 (de) * | 1987-03-20 | 1994-11-15 | Creative Biomolecules Inc | Verfahren zur reinigung von rekombinanten polypeptiden. |
| US5258496A (en) * | 1990-07-10 | 1993-11-02 | Scios Nova Inc. | Isolation and purification of lung surfactant protein |
| US5595887A (en) * | 1990-07-16 | 1997-01-21 | Bionebraska, Inc. | Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment |
| ATE253639T1 (de) * | 1991-08-19 | 2003-11-15 | Daiichi Suntory Pharma Co Ltd | Verfahren zur herstellung von peptiden |
-
1995
- 1995-12-07 CA CA 2206848 patent/CA2206848A1/fr not_active Abandoned
- 1995-12-07 AU AU44158/96A patent/AU700605B2/en not_active Ceased
- 1995-12-07 WO PCT/US1995/015800 patent/WO1996017942A1/fr not_active Application Discontinuation
- 1995-12-07 EP EP95942995A patent/EP0796335A1/fr not_active Withdrawn
- 1995-12-07 JP JP8517724A patent/JPH10510155A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JPH10510155A (ja) | 1998-10-06 |
| AU700605B2 (en) | 1999-01-07 |
| AU4415896A (en) | 1996-06-26 |
| EP0796335A1 (fr) | 1997-09-24 |
| WO1996017942A1 (fr) | 1996-06-13 |
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| Date | Code | Title | Description |
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| FZDE | Dead |